Ispitivanje efekta osmotskog stresa kod karfiola in vitro

Abstract

Cilj istraživanja je bio ispitivanje efekta osmotskog stresa izazvanog stresom soli i suše na rastenje i sadržaj suve materije kod linija karfiola gajenih in vitro. Dve odabrane linije karfiola su izložene in vitro stresu soli i suše gajenjem na MS podlozi sa 0,100, 200, 300 i 400 mM NaCl odnosno gajenjem na MS podlozi sa 0, 5,10, 15 i 20% PEG. Sveža masa biljke i sadržaj suve materije su mereni na početku i nakon 8 dana tretmana. Oba stresa su dovela do redukcije rastenja i povećanja sadržaja suve materije, ali su linije reagovale različito na pojedinačne stresore i njihove koncentracije. Linija Kf-L1 je bolje reagovala na niže koncentracije NaCl, na podlozi sa 300 mM NaCl obe linije su pokazivale sličan porast, ali na višoj koncentraciji od 400 mM linija Kf-L1 je pokazala značajno manju toleranciju na povišeni salinitet u odnosu na liniju Kf-L2. Dobijeni rezultati ukazuju na razlike među testiranim linijama u toleranciji prema stresu soli i suše u in vitro uslovima, što ovu metodu čini efikasnom u testiranju i selekciji tolerantnijih linija karfiola i drugih kupusnjača na osmotski stres.

The aim of the study was to examine the effect of osmotic stress (saltand drought stress) on growth and dry matter content in cauliflower linesgrown in vitro. Two selected cauliflower lines were exposed in vitro tosalt stress and drought by culturing on MS medium with 0, 100, 200, 300and 400 mM NaCl and resp. by culturing on MS medium with 0, 5, 10,15 and 20% PEG. The fresh weight and the dry matter content weremeasured at the beginning and after 8 days of treatment. Both stresses ledto a reduction in the growth and increase in dry matter content, but thelines reacted differently to individual stressors and their concentrations.The Kf-L1 line responded better to lower NaCl concentrations, on amedium with 300 mM NaCl both lines showed a similar increase, but at ahigher concentration of 400 mM the Kf-L1 line showed significantlylower tolerance to increased salinity compared to the Kf-L2line. Obtainedresultsindicate differences between the tested lines in tolerance to salt anddrought stress, which makes this method effective in testing and selectingmore tolerant lines of cauliflower and Brassica vegetables.