Influence of the Macrophomina phaseolina (Tassi) Goid. on SOD activity in four different genotypes of soybean seedlings

Abstract

Macrophomina phaseolina (Tassi) Goid. is a very common pest in Serbia, which affects root and lower stem in many plants, causing charcoal rot. Among other plants, soybean is also often affected by this pathogen and, like others, on the attack responds with oxidative burst. The aim of this work was to compare the sensitivity of four different genotypes of soybean (Glycine max (L.) Merr.): FAVORIT, ATLAS, SAVA and RUBIN on this pathogen. The activity of antioxidant enzyme superoxide-dismutase (SOD; EC 1.15.1.1.) was compared between four soybean genotypes, after the attack of the fungus M. phaseolina. Soybean seeds were inoculated using the method of artificial inoculation on filter paper. After seven days, seedlings were collected for biochemical assays. It was measured 1 g of plant material fresh weight and homogenized with 10 ml 0.1 M K2HPO4, pH 7.0. After centrifugation, the supernatant was used for enzyme activity measurements. The SOD activity was measured spectrophotometrically, by monitoring the inhibition of nitroblue tetrazolium (NBT) reduction at 560 nm, according to method by Mandal et al (2008).