Activity of nitrogen assimilation enzymes in soybean seedlings infected with hemibiotrophic fungi
Conference object (Published version)
Metadata
Show full item recordAbstract
The purpose of this research was to compare how soybean seedlings (Glycine max L., cultivar Bečejka) cope
with different nutrition acquisition strategies of hemibiotrophic fungi: Rhizoctonia solani Kühn and Sclerotinia
sclerotiorum (Lib.) de Bary. Severe changes at morphological and histological level after inoculation with both
fungi were accompanied by significant changes in nitrogen assimilation enzymes activities in leaves and roots
of 21-day-old soybean plants. Infected seedlings had decreased nitrate reductase (NR) (2-fold the amount of
control, on average) and glutamate synthase (GS) activity (40-60%), except in leaves infected with S. sclerotiorum.
Glutamate dehydrogenase (GDH) activity increased 46-75% after the pathogen infection, being highest
during R. solani infection. High GDH values in infected organs (0.26-0.47 μmol NADH mg-1 protein) point
to enhanced nitrogen remobilization process from infected tissue, possibly to restrict available nutrients to
pathogens, a...mong other things beneficial to plant. Differences in GS and GDH activities in the same organs
depending on pathogen infection showed that plants cope differently with these fungi at this stage of development,
or that time of switching from bio- to necrotrophic lifestyle differs between investigated pathogens.
Due to adaptable lifestyle of hemibiotrophic fungi, mechanistic details that allow pathogen to control
host metabolic pathways are unknown, and for this reason the understanding of plant nutrient acquisition
could be of great importance in the development of novel disease control strategies.
Keywords:
biotic stress / hemibiotrophic fungi / nitrogen metabolism / soybean / seedlingsSource:
Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia, 2015, 167-167Publisher:
- Belgrade: Serbian Plant Physiology Society
- Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“
Funding / projects:
Collections
Institution/Community
FiVeRTY - CONF AU - Kiprovski, Biljana AU - Malenčić, Djordje PY - 2015 UR - http://fiver.ifvcns.rs/handle/123456789/2323 AB - The purpose of this research was to compare how soybean seedlings (Glycine max L., cultivar Bečejka) cope with different nutrition acquisition strategies of hemibiotrophic fungi: Rhizoctonia solani Kühn and Sclerotinia sclerotiorum (Lib.) de Bary. Severe changes at morphological and histological level after inoculation with both fungi were accompanied by significant changes in nitrogen assimilation enzymes activities in leaves and roots of 21-day-old soybean plants. Infected seedlings had decreased nitrate reductase (NR) (2-fold the amount of control, on average) and glutamate synthase (GS) activity (40-60%), except in leaves infected with S. sclerotiorum. Glutamate dehydrogenase (GDH) activity increased 46-75% after the pathogen infection, being highest during R. solani infection. High GDH values in infected organs (0.26-0.47 μmol NADH mg-1 protein) point to enhanced nitrogen remobilization process from infected tissue, possibly to restrict available nutrients to pathogens, among other things beneficial to plant. Differences in GS and GDH activities in the same organs depending on pathogen infection showed that plants cope differently with these fungi at this stage of development, or that time of switching from bio- to necrotrophic lifestyle differs between investigated pathogens. Due to adaptable lifestyle of hemibiotrophic fungi, mechanistic details that allow pathogen to control host metabolic pathways are unknown, and for this reason the understanding of plant nutrient acquisition could be of great importance in the development of novel disease control strategies. PB - Belgrade: Serbian Plant Physiology Society PB - Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“ C3 - Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia T1 - Activity of nitrogen assimilation enzymes in soybean seedlings infected with hemibiotrophic fungi EP - 167 SP - 167 UR - https://hdl.handle.net/21.15107/rcub_fiver_2323 ER -
@conference{ author = "Kiprovski, Biljana and Malenčić, Djordje", year = "2015", abstract = "The purpose of this research was to compare how soybean seedlings (Glycine max L., cultivar Bečejka) cope with different nutrition acquisition strategies of hemibiotrophic fungi: Rhizoctonia solani Kühn and Sclerotinia sclerotiorum (Lib.) de Bary. Severe changes at morphological and histological level after inoculation with both fungi were accompanied by significant changes in nitrogen assimilation enzymes activities in leaves and roots of 21-day-old soybean plants. Infected seedlings had decreased nitrate reductase (NR) (2-fold the amount of control, on average) and glutamate synthase (GS) activity (40-60%), except in leaves infected with S. sclerotiorum. Glutamate dehydrogenase (GDH) activity increased 46-75% after the pathogen infection, being highest during R. solani infection. High GDH values in infected organs (0.26-0.47 μmol NADH mg-1 protein) point to enhanced nitrogen remobilization process from infected tissue, possibly to restrict available nutrients to pathogens, among other things beneficial to plant. Differences in GS and GDH activities in the same organs depending on pathogen infection showed that plants cope differently with these fungi at this stage of development, or that time of switching from bio- to necrotrophic lifestyle differs between investigated pathogens. Due to adaptable lifestyle of hemibiotrophic fungi, mechanistic details that allow pathogen to control host metabolic pathways are unknown, and for this reason the understanding of plant nutrient acquisition could be of great importance in the development of novel disease control strategies.", publisher = "Belgrade: Serbian Plant Physiology Society, Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“", journal = "Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia", title = "Activity of nitrogen assimilation enzymes in soybean seedlings infected with hemibiotrophic fungi", pages = "167-167", url = "https://hdl.handle.net/21.15107/rcub_fiver_2323" }
Kiprovski, B.,& Malenčić, D.. (2015). Activity of nitrogen assimilation enzymes in soybean seedlings infected with hemibiotrophic fungi. in Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia Belgrade: Serbian Plant Physiology Society., 167-167. https://hdl.handle.net/21.15107/rcub_fiver_2323
Kiprovski B, Malenčić D. Activity of nitrogen assimilation enzymes in soybean seedlings infected with hemibiotrophic fungi. in Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia. 2015;:167-167. https://hdl.handle.net/21.15107/rcub_fiver_2323 .
Kiprovski, Biljana, Malenčić, Djordje, "Activity of nitrogen assimilation enzymes in soybean seedlings infected with hemibiotrophic fungi" in Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia (2015):167-167, https://hdl.handle.net/21.15107/rcub_fiver_2323 .