Effect of Azotobacter strains on sugar beet callus proliferation and nitrogen metabolism enzymes
Апстракт
The effect of five Azotobacter chroococcum strains and nitrogen content in nutrient media on callus growth of two Beta vulgaris L. cultivars were investigated, as well as the activity of nitrate reductase (NR), glutamine synthetase (GS) and glutamate dehydrogenase (GDH) in inoculated callus tissue. On medium with full nitrogen content (1 N) the inoculation with A. chroococcum strain A(2) resulted in the highest calli mass, while strains A(8) and A(14) maximally increased NR activity. On media with 1/8 N the highest effect on calli growth, GS and GDH activity had the strain A(8). The strain A(2/1) significantly increased callus proliferation on medium without N. Asymbiotic association between sugar beet calli and Azotobacter depended on genotype/strain interaction and was realised in presence of different nitrogen levels.
Кључне речи:
Beta vulgaris L. / glutamate dehydrogenase / glutamine synthetase / nitrate reductaseИзвор:
Biologia Plantarum, 1997, 40, 2, 277-283Издавач:
- Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6
DOI: 10.1023/A:1001028922433
ISSN: 0006-3134
WoS: 000072467100015
Scopus: 2-s2.0-0031545587
Колекције
Институција/група
FiVeRTY - JOUR AU - Mezei, Snežana AU - Popović, M. AU - Kovačev, Lazar AU - Mrkovački, Nastasija AU - Nagl, Nevena AU - Malenčić, Ðorđe PY - 1997 UR - http://fiver.ifvcns.rs/handle/123456789/21 AB - The effect of five Azotobacter chroococcum strains and nitrogen content in nutrient media on callus growth of two Beta vulgaris L. cultivars were investigated, as well as the activity of nitrate reductase (NR), glutamine synthetase (GS) and glutamate dehydrogenase (GDH) in inoculated callus tissue. On medium with full nitrogen content (1 N) the inoculation with A. chroococcum strain A(2) resulted in the highest calli mass, while strains A(8) and A(14) maximally increased NR activity. On media with 1/8 N the highest effect on calli growth, GS and GDH activity had the strain A(8). The strain A(2/1) significantly increased callus proliferation on medium without N. Asymbiotic association between sugar beet calli and Azotobacter depended on genotype/strain interaction and was realised in presence of different nitrogen levels. PB - Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6 T2 - Biologia Plantarum T1 - Effect of Azotobacter strains on sugar beet callus proliferation and nitrogen metabolism enzymes EP - 283 IS - 2 SP - 277 VL - 40 DO - 10.1023/A:1001028922433 ER -
@article{ author = "Mezei, Snežana and Popović, M. and Kovačev, Lazar and Mrkovački, Nastasija and Nagl, Nevena and Malenčić, Ðorđe", year = "1997", abstract = "The effect of five Azotobacter chroococcum strains and nitrogen content in nutrient media on callus growth of two Beta vulgaris L. cultivars were investigated, as well as the activity of nitrate reductase (NR), glutamine synthetase (GS) and glutamate dehydrogenase (GDH) in inoculated callus tissue. On medium with full nitrogen content (1 N) the inoculation with A. chroococcum strain A(2) resulted in the highest calli mass, while strains A(8) and A(14) maximally increased NR activity. On media with 1/8 N the highest effect on calli growth, GS and GDH activity had the strain A(8). The strain A(2/1) significantly increased callus proliferation on medium without N. Asymbiotic association between sugar beet calli and Azotobacter depended on genotype/strain interaction and was realised in presence of different nitrogen levels.", publisher = "Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6", journal = "Biologia Plantarum", title = "Effect of Azotobacter strains on sugar beet callus proliferation and nitrogen metabolism enzymes", pages = "283-277", number = "2", volume = "40", doi = "10.1023/A:1001028922433" }
Mezei, S., Popović, M., Kovačev, L., Mrkovački, N., Nagl, N.,& Malenčić, Ð.. (1997). Effect of Azotobacter strains on sugar beet callus proliferation and nitrogen metabolism enzymes. in Biologia Plantarum Acad Sciences Czech Republic, Inst Experimental Botany, Praha 6., 40(2), 277-283. https://doi.org/10.1023/A:1001028922433
Mezei S, Popović M, Kovačev L, Mrkovački N, Nagl N, Malenčić Ð. Effect of Azotobacter strains on sugar beet callus proliferation and nitrogen metabolism enzymes. in Biologia Plantarum. 1997;40(2):277-283. doi:10.1023/A:1001028922433 .
Mezei, Snežana, Popović, M., Kovačev, Lazar, Mrkovački, Nastasija, Nagl, Nevena, Malenčić, Ðorđe, "Effect of Azotobacter strains on sugar beet callus proliferation and nitrogen metabolism enzymes" in Biologia Plantarum, 40, no. 2 (1997):277-283, https://doi.org/10.1023/A:1001028922433 . .