Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)
2019
Аутори
Ghimire, KrishnaPetrović, Kristina
Kontz, Brian
Bradley, Carl A.
Chilvers, Martin I.
Mueller, Daren S.
Smith, Damon L.
Wise, Kiersten A.
Mathew, Febina M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P lt 0.001) and pathogen recovery (P lt 0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate x method interaction affected disease severity (P lt 0.05) and pathogen recovery (P lt 0.001). Significant differ...ences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate x method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies.
Кључне речи:
Diaporthe / isolates / soybeanИзвор:
Plant Disease, 2019, 103, 4, 677-684Издавач:
- Amer Phytopathological Soc, St Paul
Финансирање / пројекти:
- North Central Soybean Research Program
- South Dakota Soybean Research and Promotion Council
- South Dakota Agricultural Experimental Station [H527-14]
DOI: 10.1094/PDIS-06-18-1078-RE
ISSN: 0191-2917
PubMed: 30742552
WoS: 000462807700074
Scopus: 2-s2.0-85064722090
Колекције
Институција/група
FiVeRTY - JOUR AU - Ghimire, Krishna AU - Petrović, Kristina AU - Kontz, Brian AU - Bradley, Carl A. AU - Chilvers, Martin I. AU - Mueller, Daren S. AU - Smith, Damon L. AU - Wise, Kiersten A. AU - Mathew, Febina M. PY - 2019 UR - http://fiver.ifvcns.rs/handle/123456789/1915 AB - One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P lt 0.001) and pathogen recovery (P lt 0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate x method interaction affected disease severity (P lt 0.05) and pathogen recovery (P lt 0.001). Significant differences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate x method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies. PB - Amer Phytopathological Soc, St Paul T2 - Plant Disease T1 - Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max) EP - 684 IS - 4 SP - 677 VL - 103 DO - 10.1094/PDIS-06-18-1078-RE ER -
@article{ author = "Ghimire, Krishna and Petrović, Kristina and Kontz, Brian and Bradley, Carl A. and Chilvers, Martin I. and Mueller, Daren S. and Smith, Damon L. and Wise, Kiersten A. and Mathew, Febina M.", year = "2019", abstract = "One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P lt 0.001) and pathogen recovery (P lt 0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate x method interaction affected disease severity (P lt 0.05) and pathogen recovery (P lt 0.001). Significant differences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate x method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies.", publisher = "Amer Phytopathological Soc, St Paul", journal = "Plant Disease", title = "Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)", pages = "684-677", number = "4", volume = "103", doi = "10.1094/PDIS-06-18-1078-RE" }
Ghimire, K., Petrović, K., Kontz, B., Bradley, C. A., Chilvers, M. I., Mueller, D. S., Smith, D. L., Wise, K. A.,& Mathew, F. M.. (2019). Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max). in Plant Disease Amer Phytopathological Soc, St Paul., 103(4), 677-684. https://doi.org/10.1094/PDIS-06-18-1078-RE
Ghimire K, Petrović K, Kontz B, Bradley CA, Chilvers MI, Mueller DS, Smith DL, Wise KA, Mathew FM. Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max). in Plant Disease. 2019;103(4):677-684. doi:10.1094/PDIS-06-18-1078-RE .
Ghimire, Krishna, Petrović, Kristina, Kontz, Brian, Bradley, Carl A., Chilvers, Martin I., Mueller, Daren S., Smith, Damon L., Wise, Kiersten A., Mathew, Febina M., "Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)" in Plant Disease, 103, no. 4 (2019):677-684, https://doi.org/10.1094/PDIS-06-18-1078-RE . .