Utilization of M. sativa ssp caerulea x M. sativa ssp sativa hybridization in improvement of alfalfa aluminium tolerance
Само за регистроване кориснике
2018
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Sensitivity of alfalfa to acidity and aluminium (Al) toxicity in soil is the major limiting factor in broadening of its growing area. Due to lack of Al tolerance in primary alfalfa germplasm, there is a need for transfer of genes for Al tolerance from other Medicago germplasm. One of the identified sources of Al tolerance is M. sativa ssp. caerulea accession PI 464724, which was used as a female parent in our study. The objectives of this study were: (i) obtaining the tetraploid offspring from 2x-4x M. sativa ssp. caerulea - M. sativa ssp. sativa spontaneous crosses, and (ii) development of a breeding strategy for Al/acid tolerance in alfalfa, using M. sativa ssp. caerulea as a source of Al tolerance. Out of eleven fully developed plants, five were morphologically similar to M. sativa ssp. caerulae, while six plants were similar to M. sativa ssp. sativa. All tested plants were fertile, with the pollen viability ranging from 21.45 to 97.09% and the average number of ovules per plant fro...m 8.80 to 12.29. Eleven SSR primer pairs confirmed the hybrid nature of M. sativa ssp. caerulae x M. sativa ssp. sativa offspring. Both the Cluster Analysis and the Principal Coordinates Analysis separated plants in the caerulae type from plants in the sativa type, with one exception. Strategies based on conventional and molecular marker breeding efforts could lead towards development of tolerant alfalfa cultivars and successful crop production on acidic, Al-contaminated soils.
Кључне речи:
acid tolerance / alfalfa / crop relatives / genetic resources / SSR markersИзвор:
Plant Genetic Resources-Characterization & Utilization, 2018, 16, 1, 68-73Издавач:
- Cambridge Univ Press, Cambridge
Финансирање / пројекти:
- Повећање тржишног значаја крмних биљака оплемењивањем и оптимизацијом технологије производње семена (RS-MESTD-Technological Development (TD or TR)-31024)
DOI: 10.1017/S1479262116000447
ISSN: 1479-2621
WoS: 000429270500008
Scopus: 2-s2.0-84997724694
Колекције
Институција/група
FiVeRTY - JOUR AU - Milić, Dragan AU - Taški-Ajduković, Ksenija AU - Nagl, Nevena AU - Atlagić, Jovanka AU - Karagić, Đura PY - 2018 UR - http://fiver.ifvcns.rs/handle/123456789/1779 AB - Sensitivity of alfalfa to acidity and aluminium (Al) toxicity in soil is the major limiting factor in broadening of its growing area. Due to lack of Al tolerance in primary alfalfa germplasm, there is a need for transfer of genes for Al tolerance from other Medicago germplasm. One of the identified sources of Al tolerance is M. sativa ssp. caerulea accession PI 464724, which was used as a female parent in our study. The objectives of this study were: (i) obtaining the tetraploid offspring from 2x-4x M. sativa ssp. caerulea - M. sativa ssp. sativa spontaneous crosses, and (ii) development of a breeding strategy for Al/acid tolerance in alfalfa, using M. sativa ssp. caerulea as a source of Al tolerance. Out of eleven fully developed plants, five were morphologically similar to M. sativa ssp. caerulae, while six plants were similar to M. sativa ssp. sativa. All tested plants were fertile, with the pollen viability ranging from 21.45 to 97.09% and the average number of ovules per plant from 8.80 to 12.29. Eleven SSR primer pairs confirmed the hybrid nature of M. sativa ssp. caerulae x M. sativa ssp. sativa offspring. Both the Cluster Analysis and the Principal Coordinates Analysis separated plants in the caerulae type from plants in the sativa type, with one exception. Strategies based on conventional and molecular marker breeding efforts could lead towards development of tolerant alfalfa cultivars and successful crop production on acidic, Al-contaminated soils. PB - Cambridge Univ Press, Cambridge T2 - Plant Genetic Resources-Characterization & Utilization T1 - Utilization of M. sativa ssp caerulea x M. sativa ssp sativa hybridization in improvement of alfalfa aluminium tolerance EP - 73 IS - 1 SP - 68 VL - 16 DO - 10.1017/S1479262116000447 ER -
@article{ author = "Milić, Dragan and Taški-Ajduković, Ksenija and Nagl, Nevena and Atlagić, Jovanka and Karagić, Đura", year = "2018", abstract = "Sensitivity of alfalfa to acidity and aluminium (Al) toxicity in soil is the major limiting factor in broadening of its growing area. Due to lack of Al tolerance in primary alfalfa germplasm, there is a need for transfer of genes for Al tolerance from other Medicago germplasm. One of the identified sources of Al tolerance is M. sativa ssp. caerulea accession PI 464724, which was used as a female parent in our study. The objectives of this study were: (i) obtaining the tetraploid offspring from 2x-4x M. sativa ssp. caerulea - M. sativa ssp. sativa spontaneous crosses, and (ii) development of a breeding strategy for Al/acid tolerance in alfalfa, using M. sativa ssp. caerulea as a source of Al tolerance. Out of eleven fully developed plants, five were morphologically similar to M. sativa ssp. caerulae, while six plants were similar to M. sativa ssp. sativa. All tested plants were fertile, with the pollen viability ranging from 21.45 to 97.09% and the average number of ovules per plant from 8.80 to 12.29. Eleven SSR primer pairs confirmed the hybrid nature of M. sativa ssp. caerulae x M. sativa ssp. sativa offspring. Both the Cluster Analysis and the Principal Coordinates Analysis separated plants in the caerulae type from plants in the sativa type, with one exception. Strategies based on conventional and molecular marker breeding efforts could lead towards development of tolerant alfalfa cultivars and successful crop production on acidic, Al-contaminated soils.", publisher = "Cambridge Univ Press, Cambridge", journal = "Plant Genetic Resources-Characterization & Utilization", title = "Utilization of M. sativa ssp caerulea x M. sativa ssp sativa hybridization in improvement of alfalfa aluminium tolerance", pages = "73-68", number = "1", volume = "16", doi = "10.1017/S1479262116000447" }
Milić, D., Taški-Ajduković, K., Nagl, N., Atlagić, J.,& Karagić, Đ.. (2018). Utilization of M. sativa ssp caerulea x M. sativa ssp sativa hybridization in improvement of alfalfa aluminium tolerance. in Plant Genetic Resources-Characterization & Utilization Cambridge Univ Press, Cambridge., 16(1), 68-73. https://doi.org/10.1017/S1479262116000447
Milić D, Taški-Ajduković K, Nagl N, Atlagić J, Karagić Đ. Utilization of M. sativa ssp caerulea x M. sativa ssp sativa hybridization in improvement of alfalfa aluminium tolerance. in Plant Genetic Resources-Characterization & Utilization. 2018;16(1):68-73. doi:10.1017/S1479262116000447 .
Milić, Dragan, Taški-Ajduković, Ksenija, Nagl, Nevena, Atlagić, Jovanka, Karagić, Đura, "Utilization of M. sativa ssp caerulea x M. sativa ssp sativa hybridization in improvement of alfalfa aluminium tolerance" in Plant Genetic Resources-Characterization & Utilization, 16, no. 1 (2018):68-73, https://doi.org/10.1017/S1479262116000447 . .