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Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil

dc.creatorDimitrijević, Aleksandra
dc.creatorImerovski, Ivana
dc.creatorMiladinović, Dragana
dc.creatorJocić, Siniša
dc.creatorCvejić, Sandra
dc.creatorMalidža, Goran
dc.creatorKovačević, Branislav
dc.creatorŠurlan-Momirović, Gordana
dc.date.accessioned2021-04-26T19:23:39Z
dc.date.available2021-04-26T19:23:39Z
dc.date.issued2016
dc.identifier.issn1821-3944
dc.identifier.urihttp://fiver.ifvcns.rs/handle/123456789/1526
dc.description.abstractA rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively.en
dc.description.abstractRazvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a morfološki parametar masa korena.sr
dc.publisherInstitut za ratarstvo i povrtarstvo, Novi Sad
dc.relationinfo:eu-repo/grantAgreement/MESTD/Technological Development (TD or TR)/31025/RS//
dc.rightsopenAccess
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceRatarstvo i povrtarstvo / Field and Vegetable Crops Research
dc.subjectbioassayen
dc.subjectHelianthus annuus L.en
dc.subjectherbicidesen
dc.subjectselectionen
dc.subjectsulfonylureasen
dc.subjecttoleranceen
dc.subjectbiološka probasr
dc.subjectHelianthus annuus L.sr
dc.subjectherbicidisr
dc.subjectselekcijasr
dc.subjectsulfonilureesr
dc.subjecttolerantnostsr
dc.titleA rapid test for detection of tribenuron-methyl resistance in sunfloweren
dc.titleBrzi test za ispitivanje otpornosti suncokreta na tribenuron metilsr
dc.typearticle
dc.rights.licenseBY
dc.citation.epage8
dc.citation.issue1
dc.citation.other53(1): 1-8
dc.citation.rankM51
dc.citation.spage1
dc.citation.volume53
dc.identifier.doi10.5937/ratpov53-8412
dc.identifier.fulltexthttp://fiver.ifvcns.rs/bitstream/id/455/1523.pdf
dc.type.versionpublishedVersion


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