Effect of auxins on mechanical injury induced oxidative stress in Prunus sp. Rootstocks

Abstract

The effect of exogenously applied auxins on oxidative stress was studied in six rootstock selections of Prunus cerasus, P. mahaleb and P. fruticosa aiming at the effect of phytohormones on the level of the lipid peroxidation (LP). Standard rootstock PHL-A was used as a control. Leaves were collected on 0, 1st, 3rd and 7th day of inserting cuttings into the rooting substrate. Auxin mixture consisted of 0.8% α-naphthylacetic acid and 0.5% indolebutyric acid. Differences in intensity of the LP between auxin-treated and -untreated cuttings were examined. Almost all of investigated selections had lower LP intensity after auxin application (11.4-47.2%). The most prominent change was in leaves of PHL-A (49.2%). LP-lowering effect were recorded in leaves of P. fruticosa (56.9%) on 7th day and in P. mahaleb on 1st and 3rd day (5.9, 5.8%). Intensity of LP could be used as one of the biochemical parameters in further rootstock selection and production.