Tolerance of spring and winter wheats to herbicide in in vitro culture

Abstract

Spring and winter wheat varieties have been compared for tolerance to tribenuron in the zygous embryo culture. Four randomply selected varieties of each spring and winter wheat were used for embryo isolation. The embryos were grown for 30 days in a modified MS (Murashige and Skoog, 1962) medium to which tribenuron was added in three different concentrations (18.75mgl-1, 37.50mgl-1, 56.25mgl-1). The percentage of surviving calli and the percentage reduction of callus fresh mass in relation to the control variant served as parameters of tolerance. All of the tested parameters indicated that the winter wheats had a higher level of tolerance to the herbicide than the spring wheats.

Ispitivane su razlike između jarih i ozimih sorti pšenice, u pogledu njihove tolerantnosti prema herbicidu tribenuron, u kulturi zigotnog embriona. Za izolaciju embriona korišćene su po četiri slučajno odabrane sorte jare i ozime pšenice. Embrioni su gajeni 30 dana na modifikovanoj MS hranljivoj podlozi (Murashige i Skoog, 1962), kojoj je tribenuron dodat u tri različite koncentracije (18.75,37,50 i 56.25 mg/L). Kao parametri tolerantnosti korišćeni su: procenat preživelih kalusa i biljaka, kao i procenat umanjenja sveže mase kalusa, u odnosu na kontrolu. Svi ispitivani parametri su pokazali viši nivo tolerantnosti ozimih sorti pšenice, prema ispitivanom herbicidu, u poređenju sa jarim sortama.