Microsatellites in the analysis of wheat genetic diversity
2014
Аутори
Kondić-Špika, AnkicaNicić, Milica
Brbaklić, Ljiljana
Trkulja, Dragana
Miladinović, Dragana
Jocić, Siniša
Brdar, Milka
Hristov, Nikola
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Microsatellite markers (SSR) were used to study wheat genetic diversity. A set of 87 wheat genotypes was analysed with four SSR markers. Primers used for the amplification of adequate microsatellite loci (Xgwm) are according to RoDER et al. (2002). Results were obtained using Applied Biosystems 3130 genetic analyser. Total of 28 alleles were determined, i.e. average of 7 alleles per marker. Number of alleles for individual markers ranged from six (Xgwm3) to eight (Xgwm18). The presence of two null alleles for Xgwm18 and Xgwm155 was found. There were five rare alleles (frequency lt 2%). Polymorphism information content (PIC) values ranged from 0.52 for Xgwm408 to 0.80 for Xgwm18. Mean PIC value was 0.69 for all markers, which signifies a high level of the detected polymorphism. According to the data collected through the analysis of four markers, most genotypes can be grouped in clusters. The results show usefulness of microsatellite markers in detecting polymorphism, identifying genot...ypes and assessing genetic diversity.
Кључне речи:
genetic variability / SSR markers / Triticum aestivum L.Извор:
Genetika-Belgrade, 2014, 46, 3, 1047-1063Издавач:
- Društvo genetičara Srbije, Beograd
Финансирање / пројекти:
- Савремено оплемењивање стрних жита за садашње и будуће потребе (RS-MESTD-Technological Development (TD or TR)-31066)
DOI: 10.2298/GENSR1403047K
ISSN: 0534-0012
WoS: 000348608200035
Scopus: 2-s2.0-84922168525
Колекције
Институција/група
FiVeRTY - JOUR AU - Kondić-Špika, Ankica AU - Nicić, Milica AU - Brbaklić, Ljiljana AU - Trkulja, Dragana AU - Miladinović, Dragana AU - Jocić, Siniša AU - Brdar, Milka AU - Hristov, Nikola PY - 2014 UR - http://fiver.ifvcns.rs/handle/123456789/1381 AB - Microsatellite markers (SSR) were used to study wheat genetic diversity. A set of 87 wheat genotypes was analysed with four SSR markers. Primers used for the amplification of adequate microsatellite loci (Xgwm) are according to RoDER et al. (2002). Results were obtained using Applied Biosystems 3130 genetic analyser. Total of 28 alleles were determined, i.e. average of 7 alleles per marker. Number of alleles for individual markers ranged from six (Xgwm3) to eight (Xgwm18). The presence of two null alleles for Xgwm18 and Xgwm155 was found. There were five rare alleles (frequency lt 2%). Polymorphism information content (PIC) values ranged from 0.52 for Xgwm408 to 0.80 for Xgwm18. Mean PIC value was 0.69 for all markers, which signifies a high level of the detected polymorphism. According to the data collected through the analysis of four markers, most genotypes can be grouped in clusters. The results show usefulness of microsatellite markers in detecting polymorphism, identifying genotypes and assessing genetic diversity. PB - Društvo genetičara Srbije, Beograd T2 - Genetika-Belgrade T1 - Microsatellites in the analysis of wheat genetic diversity EP - 1063 IS - 3 SP - 1047 VL - 46 DO - 10.2298/GENSR1403047K ER -
@article{ author = "Kondić-Špika, Ankica and Nicić, Milica and Brbaklić, Ljiljana and Trkulja, Dragana and Miladinović, Dragana and Jocić, Siniša and Brdar, Milka and Hristov, Nikola", year = "2014", abstract = "Microsatellite markers (SSR) were used to study wheat genetic diversity. A set of 87 wheat genotypes was analysed with four SSR markers. Primers used for the amplification of adequate microsatellite loci (Xgwm) are according to RoDER et al. (2002). Results were obtained using Applied Biosystems 3130 genetic analyser. Total of 28 alleles were determined, i.e. average of 7 alleles per marker. Number of alleles for individual markers ranged from six (Xgwm3) to eight (Xgwm18). The presence of two null alleles for Xgwm18 and Xgwm155 was found. There were five rare alleles (frequency lt 2%). Polymorphism information content (PIC) values ranged from 0.52 for Xgwm408 to 0.80 for Xgwm18. Mean PIC value was 0.69 for all markers, which signifies a high level of the detected polymorphism. According to the data collected through the analysis of four markers, most genotypes can be grouped in clusters. The results show usefulness of microsatellite markers in detecting polymorphism, identifying genotypes and assessing genetic diversity.", publisher = "Društvo genetičara Srbije, Beograd", journal = "Genetika-Belgrade", title = "Microsatellites in the analysis of wheat genetic diversity", pages = "1063-1047", number = "3", volume = "46", doi = "10.2298/GENSR1403047K" }
Kondić-Špika, A., Nicić, M., Brbaklić, L., Trkulja, D., Miladinović, D., Jocić, S., Brdar, M.,& Hristov, N.. (2014). Microsatellites in the analysis of wheat genetic diversity. in Genetika-Belgrade Društvo genetičara Srbije, Beograd., 46(3), 1047-1063. https://doi.org/10.2298/GENSR1403047K
Kondić-Špika A, Nicić M, Brbaklić L, Trkulja D, Miladinović D, Jocić S, Brdar M, Hristov N. Microsatellites in the analysis of wheat genetic diversity. in Genetika-Belgrade. 2014;46(3):1047-1063. doi:10.2298/GENSR1403047K .
Kondić-Špika, Ankica, Nicić, Milica, Brbaklić, Ljiljana, Trkulja, Dragana, Miladinović, Dragana, Jocić, Siniša, Brdar, Milka, Hristov, Nikola, "Microsatellites in the analysis of wheat genetic diversity" in Genetika-Belgrade, 46, no. 3 (2014):1047-1063, https://doi.org/10.2298/GENSR1403047K . .