FiVeR - Repository of the Institute of Field and Vegetable Crops
Institute of Field and Vegetable Crops
    • English
    • Српски
    • Српски (Serbia)
  • English 
    • English
    • Serbian (Cyrillic)
    • Serbian (Latin)
  • Login
View Item 
  •   FiVeR
  • FiVeR
  • Radovi istraživača / Researchers' papers
  • View Item
  •   FiVeR
  • FiVeR
  • Radovi istraživača / Researchers' papers
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean

Thumbnail
2014
1299.pdf (717.0Kb)
Authors
Petrović, Gordana
Nikolić, Zorica
Đorđević, Vuk
Župunski, Vesna
Jovičić, Dušica
Ignjatov, Maja
Milošević, Dragana
Article (Published version)
Metadata
Show full item record
Abstract
The objective of this work was to evaluate the genetic variation of trypsin inhibitor in cultivated (Glycine max L.) and wild (Glycine sofa Siebold & Zucc.) soybean varieties. Genetic variations of the Kunitz trypsin inhibitor, represented by a 21-kD protein (KTI), and of the Bowman-Birk trypsin chymotrypsin inhibitor (BBI) were evaluated in cultivated (G. max) and wild (G. sofa) soybean varieties. Endonuclease heteroduplex mismatch cleavage assays were performed to detect mutations in the KTI gene, with a single-stranded specific nuclease obtained from celery extracts (CEL I). The investigated soybean varieties showed low level of genetic variation in KTI and BBI. PCR-RFLP analysis divided the BBI-A type into subtypes A1 and A2, and showed that Tib type of KTI is the dominant type. Digestion with restriction enzymes was not able to detect differences between ti-null and other types of Ti alleles, while the endonuclease heteroduplex mismatch cleavage assay with CEL I could detect ti-nu...ll type. The digestion method with CEL I provides a simple and useful genetic tool for SNP analysis. The presented method can be used as a tool for fast and useful screening of desired genotypes in future breeding programs of soybean.

Keywords:
Glycine max / Glycine soja / antinutritional factors / protease inhibitors / SNP
Source:
Pesquisa Agropecuaria Brasileira, 2014, 49, 2, 102-108
Publisher:
  • Empresa Brasil Pesq Agropec, Brasilia Df
Funding / projects:
  • Increasing the market significance of forage crops by breeding and optimizing seed production technology (RS-31024)
  • Interdisciplinary Approach to Development of New Soybean Varieties and Improvement of the Cultivation Practices and Seed Production (RS-31022)

DOI: 10.1590/S0100-204X2014000200004

ISSN: 0100-204X

WoS: 000337718300004

Scopus: 2-s2.0-84903450881
[ Google Scholar ]
1
URI
http://fiver.ifvcns.rs/handle/123456789/1302
Collections
  • Radovi istraživača / Researchers' papers
Institution/Community
FiVeR
TY  - JOUR
AU  - Petrović, Gordana
AU  - Nikolić, Zorica
AU  - Đorđević, Vuk
AU  - Župunski, Vesna
AU  - Jovičić, Dušica
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1302
AB  - The objective of this work was to evaluate the genetic variation of trypsin inhibitor in cultivated (Glycine max L.) and wild (Glycine sofa Siebold & Zucc.) soybean varieties. Genetic variations of the Kunitz trypsin inhibitor, represented by a 21-kD protein (KTI), and of the Bowman-Birk trypsin chymotrypsin inhibitor (BBI) were evaluated in cultivated (G. max) and wild (G. sofa) soybean varieties. Endonuclease heteroduplex mismatch cleavage assays were performed to detect mutations in the KTI gene, with a single-stranded specific nuclease obtained from celery extracts (CEL I). The investigated soybean varieties showed low level of genetic variation in KTI and BBI. PCR-RFLP analysis divided the BBI-A type into subtypes A1 and A2, and showed that Tib type of KTI is the dominant type. Digestion with restriction enzymes was not able to detect differences between ti-null and other types of Ti alleles, while the endonuclease heteroduplex mismatch cleavage assay with CEL I could detect ti-null type. The digestion method with CEL I provides a simple and useful genetic tool for SNP analysis. The presented method can be used as a tool for fast and useful screening of desired genotypes in future breeding programs of soybean.
PB  - Empresa Brasil Pesq Agropec, Brasilia Df
T2  - Pesquisa Agropecuaria Brasileira
T1  - Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean
EP  - 108
IS  - 2
SP  - 102
VL  - 49
DO  - 10.1590/S0100-204X2014000200004
UR  - conv_1991
ER  - 
@article{
author = "Petrović, Gordana and Nikolić, Zorica and Đorđević, Vuk and Župunski, Vesna and Jovičić, Dušica and Ignjatov, Maja and Milošević, Dragana",
year = "2014",
abstract = "The objective of this work was to evaluate the genetic variation of trypsin inhibitor in cultivated (Glycine max L.) and wild (Glycine sofa Siebold & Zucc.) soybean varieties. Genetic variations of the Kunitz trypsin inhibitor, represented by a 21-kD protein (KTI), and of the Bowman-Birk trypsin chymotrypsin inhibitor (BBI) were evaluated in cultivated (G. max) and wild (G. sofa) soybean varieties. Endonuclease heteroduplex mismatch cleavage assays were performed to detect mutations in the KTI gene, with a single-stranded specific nuclease obtained from celery extracts (CEL I). The investigated soybean varieties showed low level of genetic variation in KTI and BBI. PCR-RFLP analysis divided the BBI-A type into subtypes A1 and A2, and showed that Tib type of KTI is the dominant type. Digestion with restriction enzymes was not able to detect differences between ti-null and other types of Ti alleles, while the endonuclease heteroduplex mismatch cleavage assay with CEL I could detect ti-null type. The digestion method with CEL I provides a simple and useful genetic tool for SNP analysis. The presented method can be used as a tool for fast and useful screening of desired genotypes in future breeding programs of soybean.",
publisher = "Empresa Brasil Pesq Agropec, Brasilia Df",
journal = "Pesquisa Agropecuaria Brasileira",
title = "Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean",
pages = "108-102",
number = "2",
volume = "49",
doi = "10.1590/S0100-204X2014000200004",
url = "conv_1991"
}
Petrović, G., Nikolić, Z., Đorđević, V., Župunski, V., Jovičić, D., Ignjatov, M.,& Milošević, D.. (2014). Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean. in Pesquisa Agropecuaria Brasileira
Empresa Brasil Pesq Agropec, Brasilia Df., 49(2), 102-108.
https://doi.org/10.1590/S0100-204X2014000200004
conv_1991
Petrović G, Nikolić Z, Đorđević V, Župunski V, Jovičić D, Ignjatov M, Milošević D. Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean. in Pesquisa Agropecuaria Brasileira. 2014;49(2):102-108.
doi:10.1590/S0100-204X2014000200004
conv_1991 .
Petrović, Gordana, Nikolić, Zorica, Đorđević, Vuk, Župunski, Vesna, Jovičić, Dušica, Ignjatov, Maja, Milošević, Dragana, "Endonuclease heteroduplex mismatch cleavage for detecting mutation genetic variation of trypsin inhibitors in soybean" in Pesquisa Agropecuaria Brasileira, 49, no. 2 (2014):102-108,
https://doi.org/10.1590/S0100-204X2014000200004 .,
conv_1991 .

DSpace software copyright © 2002-2015  DuraSpace
About FiVeR | Send Feedback

OpenAIRERCUB
 

 

All of DSpaceInstitutions/communitiesAuthorsTitlesSubjectsThis institutionAuthorsTitlesSubjects

Statistics

View Usage Statistics

DSpace software copyright © 2002-2015  DuraSpace
About FiVeR | Send Feedback

OpenAIRERCUB