Legumes seed storage proteins characterization by SDS-PAGE and Lab-on-a-Chip electrophoresis
Abstract
This study was conducted to analyze the seed storage proteins of grain legumes cultivars using Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Lab-on-a-Chip (LoaC) electrophoresis. Using reference proteins, the relative accuracy and precision in estimating molecular weight was similar when these two methods were compared. However, the LoaC method had a shorter analysis time and higher resolution, demonstrating a significant advantage. The molecular weight estimation of grain legume storage proteins gave very similar results (R-2 = 0.99). Relative quantification of the separated proteins also indicated good agreement of the two techniques (R-2 = 0.81). Both methods can be used to analyze the composition of the legume seed proteins, but the LoaC method is more suited to the separation and detection of proteins, enabling low reagent consumption, easy operation and faster turnaround. The LoaC method seems suitable for legume quality screening and cultivar developme...nt where large numbers of samples need to be analyzed.
Keywords:
Grain legumes / Seed storage proteins / Lab-on-the-Chip / SDS-PAGE / Analytical method comparison / Food analysis / Food compositionSource:
Journal of Food Composition & Analysis, 2012, 28, 2, 75-80Publisher:
- Academic Press Inc Elsevier Science, San Diego
Funding / projects:
DOI: 10.1016/j.jfca.2012.08.005
ISSN: 0889-1575
WoS: 000313772300001
Scopus: 2-s2.0-84869047364
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Institution/Community
FiVeRTY - JOUR AU - Nikolić, Zorica AU - Đorđević, Vuk AU - Torbica, Aleksandra AU - Mikić, Aleksandar PY - 2012 UR - http://fiver.ifvcns.rs/handle/123456789/1084 AB - This study was conducted to analyze the seed storage proteins of grain legumes cultivars using Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Lab-on-a-Chip (LoaC) electrophoresis. Using reference proteins, the relative accuracy and precision in estimating molecular weight was similar when these two methods were compared. However, the LoaC method had a shorter analysis time and higher resolution, demonstrating a significant advantage. The molecular weight estimation of grain legume storage proteins gave very similar results (R-2 = 0.99). Relative quantification of the separated proteins also indicated good agreement of the two techniques (R-2 = 0.81). Both methods can be used to analyze the composition of the legume seed proteins, but the LoaC method is more suited to the separation and detection of proteins, enabling low reagent consumption, easy operation and faster turnaround. The LoaC method seems suitable for legume quality screening and cultivar development where large numbers of samples need to be analyzed. PB - Academic Press Inc Elsevier Science, San Diego T2 - Journal of Food Composition & Analysis T1 - Legumes seed storage proteins characterization by SDS-PAGE and Lab-on-a-Chip electrophoresis EP - 80 IS - 2 SP - 75 VL - 28 DO - 10.1016/j.jfca.2012.08.005 ER -
@article{ author = "Nikolić, Zorica and Đorđević, Vuk and Torbica, Aleksandra and Mikić, Aleksandar", year = "2012", abstract = "This study was conducted to analyze the seed storage proteins of grain legumes cultivars using Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Lab-on-a-Chip (LoaC) electrophoresis. Using reference proteins, the relative accuracy and precision in estimating molecular weight was similar when these two methods were compared. However, the LoaC method had a shorter analysis time and higher resolution, demonstrating a significant advantage. The molecular weight estimation of grain legume storage proteins gave very similar results (R-2 = 0.99). Relative quantification of the separated proteins also indicated good agreement of the two techniques (R-2 = 0.81). Both methods can be used to analyze the composition of the legume seed proteins, but the LoaC method is more suited to the separation and detection of proteins, enabling low reagent consumption, easy operation and faster turnaround. The LoaC method seems suitable for legume quality screening and cultivar development where large numbers of samples need to be analyzed.", publisher = "Academic Press Inc Elsevier Science, San Diego", journal = "Journal of Food Composition & Analysis", title = "Legumes seed storage proteins characterization by SDS-PAGE and Lab-on-a-Chip electrophoresis", pages = "80-75", number = "2", volume = "28", doi = "10.1016/j.jfca.2012.08.005" }
Nikolić, Z., Đorđević, V., Torbica, A.,& Mikić, A.. (2012). Legumes seed storage proteins characterization by SDS-PAGE and Lab-on-a-Chip electrophoresis. in Journal of Food Composition & Analysis Academic Press Inc Elsevier Science, San Diego., 28(2), 75-80. https://doi.org/10.1016/j.jfca.2012.08.005
Nikolić Z, Đorđević V, Torbica A, Mikić A. Legumes seed storage proteins characterization by SDS-PAGE and Lab-on-a-Chip electrophoresis. in Journal of Food Composition & Analysis. 2012;28(2):75-80. doi:10.1016/j.jfca.2012.08.005 .
Nikolić, Zorica, Đorđević, Vuk, Torbica, Aleksandra, Mikić, Aleksandar, "Legumes seed storage proteins characterization by SDS-PAGE and Lab-on-a-Chip electrophoresis" in Journal of Food Composition & Analysis, 28, no. 2 (2012):75-80, https://doi.org/10.1016/j.jfca.2012.08.005 . .