Relationships among androgenetic components in wheat and their responses to the environment

Abstract

The effect of two nutrient media, Potato-2 and N6, on androgenetic component traits and their relationships were studied in wheat anther culture. Six wheat (Triticum aestivum L.) F-1 combinations (NS 20 x Driada, MV 05-2001 x Lana, MV 05-2001 x Zitnica, Driada x NS 403/00, Driada x Dacia and Odeska 162 x NS KV 124/01A) were used as material for anther isolation. Variations, interrelationships and path analysis for five androgenetic component traits (androgenetic capacity: AC, embryoid induction: El, regeneration ability: RA, frequency of green plants: FOP and frequency of albino plants: FAP) were established. The average values for AC and El were significantly lower on N6 medium, while for RA, FOP and FAP there were no significant differences between the Potato-2 and N6 media. Correlation coefficient analyses showed positive correlation between AC and El (r = 0.99* on Potato-2 and r = 0.86* on N6), and between RA and FOP (r = 0.95* on Potato-2, r = 0.93* on N6), on both media. Additionally, the direct effects of RA and FAP on FOP were higly significant on both media, as determined by path coefficient, analysis. A trait-association using environment biplot was applied to understand the environmental effects on these relationships. The biplot showed that anther culture trait associations were differently responsive to the environment.