Micropropagation of Helianthus maximiliani (Schrader) by shoot apex culture
Abstract
H. maximiliani was micropropagated using culture of shoot apices on modified Murashige and Skoog medium (DV). Further propagation of in vitro grown plants was done by culture of their nodal segments and shoot tips on the same medium supplemented with phloridzin, silver nitrate and casein hydrolysate (DV'). Rooting was induced by dipping the explants into IBA solution prior culture. Viable protoplasts (90%) were isolated from leaf mesophyll. These protoplasts divided (18%) in culture in agarose droplets.
Keywords:
Helianthus maximiliani / micropropagation / shoot apex culture / protoplastsSource:
Helia, 2001, 24, 34, 63-68Publisher:
- Institut za ratarstvo i povrtarstvo, Novi Sad
Collections
Institution/Community
FiVeRTY - JOUR AU - Vasić, Dragana AU - Škorić, Dragan AU - Gilbert, Alibert AU - Miklič, Vladimir PY - 2001 UR - http://fiver.ifvcns.rs/handle/123456789/101 AB - H. maximiliani was micropropagated using culture of shoot apices on modified Murashige and Skoog medium (DV). Further propagation of in vitro grown plants was done by culture of their nodal segments and shoot tips on the same medium supplemented with phloridzin, silver nitrate and casein hydrolysate (DV'). Rooting was induced by dipping the explants into IBA solution prior culture. Viable protoplasts (90%) were isolated from leaf mesophyll. These protoplasts divided (18%) in culture in agarose droplets. PB - Institut za ratarstvo i povrtarstvo, Novi Sad T2 - Helia T1 - Micropropagation of Helianthus maximiliani (Schrader) by shoot apex culture EP - 68 IS - 34 SP - 63 VL - 24 UR - https://hdl.handle.net/21.15107/rcub_fiver_101 ER -
@article{ author = "Vasić, Dragana and Škorić, Dragan and Gilbert, Alibert and Miklič, Vladimir", year = "2001", abstract = "H. maximiliani was micropropagated using culture of shoot apices on modified Murashige and Skoog medium (DV). Further propagation of in vitro grown plants was done by culture of their nodal segments and shoot tips on the same medium supplemented with phloridzin, silver nitrate and casein hydrolysate (DV'). Rooting was induced by dipping the explants into IBA solution prior culture. Viable protoplasts (90%) were isolated from leaf mesophyll. These protoplasts divided (18%) in culture in agarose droplets.", publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad", journal = "Helia", title = "Micropropagation of Helianthus maximiliani (Schrader) by shoot apex culture", pages = "68-63", number = "34", volume = "24", url = "https://hdl.handle.net/21.15107/rcub_fiver_101" }
Vasić, D., Škorić, D., Gilbert, A.,& Miklič, V.. (2001). Micropropagation of Helianthus maximiliani (Schrader) by shoot apex culture. in Helia Institut za ratarstvo i povrtarstvo, Novi Sad., 24(34), 63-68. https://hdl.handle.net/21.15107/rcub_fiver_101
Vasić D, Škorić D, Gilbert A, Miklič V. Micropropagation of Helianthus maximiliani (Schrader) by shoot apex culture. in Helia. 2001;24(34):63-68. https://hdl.handle.net/21.15107/rcub_fiver_101 .
Vasić, Dragana, Škorić, Dragan, Gilbert, Alibert, Miklič, Vladimir, "Micropropagation of Helianthus maximiliani (Schrader) by shoot apex culture" in Helia, 24, no. 34 (2001):63-68, https://hdl.handle.net/21.15107/rcub_fiver_101 .