TY  - JOUR
AU  - Ghimire, Krishna
AU  - Petrović, Kristina
AU  - Kontz, Brian
AU  - Bradley, Carl A.
AU  - Chilvers, Martin I.
AU  - Mueller, Daren S.
AU  - Smith, Damon L.
AU  - Wise, Kiersten A.
AU  - Mathew, Febina M.
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1915
AB  - One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P  lt  0.001) and pathogen recovery (P  lt  0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate x method interaction affected disease severity (P  lt  0.05) and pathogen recovery (P  lt  0.001). Significant differences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate x method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)
EP  - 684
IS  - 4
SP  - 677
VL  - 103
DO  - 10.1094/PDIS-06-18-1078-RE
ER  - 

@article{
author = "Ghimire, Krishna and Petrović, Kristina and Kontz, Brian and Bradley, Carl A. and Chilvers, Martin I. and Mueller, Daren S. and Smith, Damon L. and Wise, Kiersten A. and Mathew, Febina M.",
year = "2019",
abstract = "One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P  lt  0.001) and pathogen recovery (P  lt  0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate x method interaction affected disease severity (P  lt  0.05) and pathogen recovery (P  lt  0.001). Significant differences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate x method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)",
pages = "684-677",
number = "4",
volume = "103",
doi = "10.1094/PDIS-06-18-1078-RE"
}

Ghimire, K., Petrović, K., Kontz, B., Bradley, C. A., Chilvers, M. I., Mueller, D. S., Smith, D. L., Wise, K. A.,& Mathew, F. M.. (2019). Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max). in Plant Disease
Amer Phytopathological Soc, St Paul., 103(4), 677-684.
https://doi.org/10.1094/PDIS-06-18-1078-RE

Ghimire K, Petrović K, Kontz B, Bradley CA, Chilvers MI, Mueller DS, Smith DL, Wise KA, Mathew FM. Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max). in Plant Disease. 2019;103(4):677-684.
doi:10.1094/PDIS-06-18-1078-RE .

Ghimire, Krishna, Petrović, Kristina, Kontz, Brian, Bradley, Carl A., Chilvers, Martin I., Mueller, Daren S., Smith, Damon L., Wise, Kiersten A., Mathew, Febina M., "Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)" in Plant Disease, 103, no. 4 (2019):677-684,
https://doi.org/10.1094/PDIS-06-18-1078-RE . .

TY  - JOUR
AU  - Okello, Paul N.
AU  - Petrović, Kristina
AU  - Kontz, Brian
AU  - Mathew, Febina M.
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1882
AB  - Fusarium root rot of corn (Zea mays L.) is yield-limiting in the United States, but there is no information available on the disease in South Dakota. In 2015, corn seedlings with discolored roots were arbitrarily sampled from 50 South Dakota fields, and 198 isolates were recovered. Eight species (F. acuminatum, F. boothii, F. equiseti-incarnatum complex, F. graminearum, F. oxysporum, F. proliferatum, F. solani, and F. subglutinans) were identified by morphology and translation elongation factor 1-alpha gene sequencing. F. graminearum (26.8%) was the most common fungus, and F. boothii (0.5%) was the least recovered. Fourteen isolates, representing the eight species, were evaluated for their pathogenicity on 2-week-old seedlings of inbred 'B73' using the inoculum layer method in the greenhouse. Fourteen days post-inoculation, root rot severity was evaluated on a 1-to-5 rating scale and expressed as relative treatment effects (RTES). F. proliferatum isolate P2 caused significantly greater RTE (based on 95% confidence intervals) on seedlings than the other isolates and the noninoculated control, except F. graminearum isolate FG23. This study indicates that the eight species of Fusarium are aggressive root rot pathogens of corn in South Dakota, and this information will help evaluate strategies for producers to manage these pathogens in their fields.
PB  - Plant Management Network, St Paul
T2  - Plant Health Progress
T1  - Eight Species of Fusarium Cause Root Rot of Corn (Zea mays) in South Dakota
EP  - 43
IS  - 1
SP  - 38
VL  - 20
DO  - 10.1094/PHP-11-18-0075-RS
ER  - 

@article{
author = "Okello, Paul N. and Petrović, Kristina and Kontz, Brian and Mathew, Febina M.",
year = "2019",
abstract = "Fusarium root rot of corn (Zea mays L.) is yield-limiting in the United States, but there is no information available on the disease in South Dakota. In 2015, corn seedlings with discolored roots were arbitrarily sampled from 50 South Dakota fields, and 198 isolates were recovered. Eight species (F. acuminatum, F. boothii, F. equiseti-incarnatum complex, F. graminearum, F. oxysporum, F. proliferatum, F. solani, and F. subglutinans) were identified by morphology and translation elongation factor 1-alpha gene sequencing. F. graminearum (26.8%) was the most common fungus, and F. boothii (0.5%) was the least recovered. Fourteen isolates, representing the eight species, were evaluated for their pathogenicity on 2-week-old seedlings of inbred 'B73' using the inoculum layer method in the greenhouse. Fourteen days post-inoculation, root rot severity was evaluated on a 1-to-5 rating scale and expressed as relative treatment effects (RTES). F. proliferatum isolate P2 caused significantly greater RTE (based on 95% confidence intervals) on seedlings than the other isolates and the noninoculated control, except F. graminearum isolate FG23. This study indicates that the eight species of Fusarium are aggressive root rot pathogens of corn in South Dakota, and this information will help evaluate strategies for producers to manage these pathogens in their fields.",
publisher = "Plant Management Network, St Paul",
journal = "Plant Health Progress",
title = "Eight Species of Fusarium Cause Root Rot of Corn (Zea mays) in South Dakota",
pages = "43-38",
number = "1",
volume = "20",
doi = "10.1094/PHP-11-18-0075-RS"
}

Okello, P. N., Petrović, K., Kontz, B.,& Mathew, F. M.. (2019). Eight Species of Fusarium Cause Root Rot of Corn (Zea mays) in South Dakota. in Plant Health Progress
Plant Management Network, St Paul., 20(1), 38-43.
https://doi.org/10.1094/PHP-11-18-0075-RS

Okello PN, Petrović K, Kontz B, Mathew FM. Eight Species of Fusarium Cause Root Rot of Corn (Zea mays) in South Dakota. in Plant Health Progress. 2019;20(1):38-43.
doi:10.1094/PHP-11-18-0075-RS .

Okello, Paul N., Petrović, Kristina, Kontz, Brian, Mathew, Febina M., "Eight Species of Fusarium Cause Root Rot of Corn (Zea mays) in South Dakota" in Plant Health Progress, 20, no. 1 (2019):38-43,
https://doi.org/10.1094/PHP-11-18-0075-RS . .