Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade

Link to this page

info:eu-repo/grantAgreement/EC/FP7/316004/EU//

Advancing research in agricultural and food sciences at Faculty of Agriculture, University of Belgrade (en)
Authors

Publications

Velvetleaf (Abutilon theophrasti Medik.) productivity in competitive conditions

Vrbničanin, Sava; Onc-Jovanović, Eleonora; Božić, Dragana; Sarić-Krsmanović, Marija; Pavlović, Danijela; Malidža, Goran; Jarić, Snežana

(Srpsko biološko društvo, Beograd, i dr., 2017)

TY  - JOUR
AU  - Vrbničanin, Sava
AU  - Onc-Jovanović, Eleonora
AU  - Božić, Dragana
AU  - Sarić-Krsmanović, Marija
AU  - Pavlović, Danijela
AU  - Malidža, Goran
AU  - Jarić, Snežana
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1665
AB  - Velvetleaf (Abutilon theophrasti Medik.) is an invasive alien species in many countries and one of the major weeds in summer row crops worldwide. Weed-management techniques that reduce weed production need to be investigated to provide new approaches. The first step in this process is the determination of weed productivity in different competitive conditions. Field experiments were conducted in 2006 and 2008 in an experimental field in Padinska Skela to quantify growth and seed production of velvetleaf in maize, as well as in a velvetleaf monoculture. A density of velvetleaf ranging from 1 to 8 plants m-1 was artificially created. In a mixture with maize, velvetleaf was sown in crop rows. The growth of velvetleaf was estimated based on plant height, fresh aboveground biomass and leaf area index (LAI). Velvetleaf fecundity was determined as seed mass plant(-1) and seed mass m(-2). Differences between years in plant production were very prominent. In general, velvetleaf productivity in maize depended on its density. Intraspecific competition had a major influence on growth and seed production when velvetleaf density was from 4 to 8 plants m(-1) in maize rows. This information indicates that environmental conditions and weed density can promote/reduce inter-and intraspecific competition and help in the construction of population dynamics models to predict population density, seed bank and competitiveness of weeds and reduce inputs for weed management.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Velvetleaf (Abutilon theophrasti Medik.) productivity in competitive conditions
EP  - 166
IS  - 1
SP  - 157
VL  - 69
DO  - 10.2298/ABS160212092V
ER  - 
@article{
author = "Vrbničanin, Sava and Onc-Jovanović, Eleonora and Božić, Dragana and Sarić-Krsmanović, Marija and Pavlović, Danijela and Malidža, Goran and Jarić, Snežana",
year = "2017",
abstract = "Velvetleaf (Abutilon theophrasti Medik.) is an invasive alien species in many countries and one of the major weeds in summer row crops worldwide. Weed-management techniques that reduce weed production need to be investigated to provide new approaches. The first step in this process is the determination of weed productivity in different competitive conditions. Field experiments were conducted in 2006 and 2008 in an experimental field in Padinska Skela to quantify growth and seed production of velvetleaf in maize, as well as in a velvetleaf monoculture. A density of velvetleaf ranging from 1 to 8 plants m-1 was artificially created. In a mixture with maize, velvetleaf was sown in crop rows. The growth of velvetleaf was estimated based on plant height, fresh aboveground biomass and leaf area index (LAI). Velvetleaf fecundity was determined as seed mass plant(-1) and seed mass m(-2). Differences between years in plant production were very prominent. In general, velvetleaf productivity in maize depended on its density. Intraspecific competition had a major influence on growth and seed production when velvetleaf density was from 4 to 8 plants m(-1) in maize rows. This information indicates that environmental conditions and weed density can promote/reduce inter-and intraspecific competition and help in the construction of population dynamics models to predict population density, seed bank and competitiveness of weeds and reduce inputs for weed management.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Velvetleaf (Abutilon theophrasti Medik.) productivity in competitive conditions",
pages = "166-157",
number = "1",
volume = "69",
doi = "10.2298/ABS160212092V"
}
Vrbničanin, S., Onc-Jovanović, E., Božić, D., Sarić-Krsmanović, M., Pavlović, D., Malidža, G.,& Jarić, S.. (2017). Velvetleaf (Abutilon theophrasti Medik.) productivity in competitive conditions. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 69(1), 157-166.
https://doi.org/10.2298/ABS160212092V
Vrbničanin S, Onc-Jovanović E, Božić D, Sarić-Krsmanović M, Pavlović D, Malidža G, Jarić S. Velvetleaf (Abutilon theophrasti Medik.) productivity in competitive conditions. in Archives of Biological Sciences. 2017;69(1):157-166.
doi:10.2298/ABS160212092V .
Vrbničanin, Sava, Onc-Jovanović, Eleonora, Božić, Dragana, Sarić-Krsmanović, Marija, Pavlović, Danijela, Malidža, Goran, Jarić, Snežana, "Velvetleaf (Abutilon theophrasti Medik.) productivity in competitive conditions" in Archives of Biological Sciences, 69, no. 1 (2017):157-166,
https://doi.org/10.2298/ABS160212092V . .
10
2
2

Response of wheat plants under post-anthesis stress induced by defoliation: II. Contribution of peduncle morpho-anatomical traits and carbon reserves to grain yield

Dodig, Dejan; Rančić, Dragana; Vučelić-Radović, Biljana; Zorić, Miroslav; Savić, Jasna; Kandić, Vesna; Pecinar, I.; Stanojević, S.; Šešlija, A.; Vassilev, Dimitar; Pekic-Quarrie, S.

(Cambridge Univ Press, New York, 2017)

TY  - JOUR
AU  - Dodig, Dejan
AU  - Rančić, Dragana
AU  - Vučelić-Radović, Biljana
AU  - Zorić, Miroslav
AU  - Savić, Jasna
AU  - Kandić, Vesna
AU  - Pecinar, I.
AU  - Stanojević, S.
AU  - Šešlija, A.
AU  - Vassilev, Dimitar
AU  - Pekic-Quarrie, S.
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1674
AB  - Assimilated carbon during vegetative and early reproductive growth in wheat is temporarily stored in stem internodes and leaf sheaths (LSs), and can later be remobilized and transported to developing grain. The present study was undertaken to determine the effect of several peduncle (the uppermost internode) morpho-anatomical and biochemical traits on grain weight, and to assess the contribution of the peduncle water-soluble carbohydrate (WSC) reserves shortly after anthesis to its variation. In 2-year field trials, 61 wheat genotypes were used (27 F-4:5 families, 17 parents used for the crosses and the 17 current best standards), comparing intact control plants (CP) with plants that were defoliated (DP) by cutting off all leaf blades 10 days after anthesis. Estimated contributions of peduncle (culm (C) and flag LS) assimilate reserves to grain weight/spike were from 006 to 031 and from 011 to 045 in CP and DP plants, respectively. In both CP and DP plants, a higher contribution was from the LS than from the C. High peduncle reserve mobilization efficiency, a longer exposed part of the peduncle and larger C storage capacity (through higher parenchyma and/or lower lignified area) were of specific benefit for maintaining grain weight in defoliated plants. F-4:5 families had higher transport capacity in the peduncle, but without any improvement in WSC-related traits compared with the best standards.
PB  - Cambridge Univ Press, New York
T2  - Journal of Agricultural Science
T1  - Response of wheat plants under post-anthesis stress induced by defoliation: II. Contribution of peduncle morpho-anatomical traits and carbon reserves to grain yield
EP  - 493
IS  - 3
SP  - 475
VL  - 155
DO  - 10.1017/S0021859616000551
ER  - 
@article{
author = "Dodig, Dejan and Rančić, Dragana and Vučelić-Radović, Biljana and Zorić, Miroslav and Savić, Jasna and Kandić, Vesna and Pecinar, I. and Stanojević, S. and Šešlija, A. and Vassilev, Dimitar and Pekic-Quarrie, S.",
year = "2017",
abstract = "Assimilated carbon during vegetative and early reproductive growth in wheat is temporarily stored in stem internodes and leaf sheaths (LSs), and can later be remobilized and transported to developing grain. The present study was undertaken to determine the effect of several peduncle (the uppermost internode) morpho-anatomical and biochemical traits on grain weight, and to assess the contribution of the peduncle water-soluble carbohydrate (WSC) reserves shortly after anthesis to its variation. In 2-year field trials, 61 wheat genotypes were used (27 F-4:5 families, 17 parents used for the crosses and the 17 current best standards), comparing intact control plants (CP) with plants that were defoliated (DP) by cutting off all leaf blades 10 days after anthesis. Estimated contributions of peduncle (culm (C) and flag LS) assimilate reserves to grain weight/spike were from 006 to 031 and from 011 to 045 in CP and DP plants, respectively. In both CP and DP plants, a higher contribution was from the LS than from the C. High peduncle reserve mobilization efficiency, a longer exposed part of the peduncle and larger C storage capacity (through higher parenchyma and/or lower lignified area) were of specific benefit for maintaining grain weight in defoliated plants. F-4:5 families had higher transport capacity in the peduncle, but without any improvement in WSC-related traits compared with the best standards.",
publisher = "Cambridge Univ Press, New York",
journal = "Journal of Agricultural Science",
title = "Response of wheat plants under post-anthesis stress induced by defoliation: II. Contribution of peduncle morpho-anatomical traits and carbon reserves to grain yield",
pages = "493-475",
number = "3",
volume = "155",
doi = "10.1017/S0021859616000551"
}
Dodig, D., Rančić, D., Vučelić-Radović, B., Zorić, M., Savić, J., Kandić, V., Pecinar, I., Stanojević, S., Šešlija, A., Vassilev, D.,& Pekic-Quarrie, S.. (2017). Response of wheat plants under post-anthesis stress induced by defoliation: II. Contribution of peduncle morpho-anatomical traits and carbon reserves to grain yield. in Journal of Agricultural Science
Cambridge Univ Press, New York., 155(3), 475-493.
https://doi.org/10.1017/S0021859616000551
Dodig D, Rančić D, Vučelić-Radović B, Zorić M, Savić J, Kandić V, Pecinar I, Stanojević S, Šešlija A, Vassilev D, Pekic-Quarrie S. Response of wheat plants under post-anthesis stress induced by defoliation: II. Contribution of peduncle morpho-anatomical traits and carbon reserves to grain yield. in Journal of Agricultural Science. 2017;155(3):475-493.
doi:10.1017/S0021859616000551 .
Dodig, Dejan, Rančić, Dragana, Vučelić-Radović, Biljana, Zorić, Miroslav, Savić, Jasna, Kandić, Vesna, Pecinar, I., Stanojević, S., Šešlija, A., Vassilev, Dimitar, Pekic-Quarrie, S., "Response of wheat plants under post-anthesis stress induced by defoliation: II. Contribution of peduncle morpho-anatomical traits and carbon reserves to grain yield" in Journal of Agricultural Science, 155, no. 3 (2017):475-493,
https://doi.org/10.1017/S0021859616000551 . .
10
3
9

Classification of continental halophytic grassland vegetation of Southeastern Europe

Dajić-Stevanović, Zora; Acić, Svetlana; Luković, Milica; Zlatković, Ivana; Vasin, Jovica; Topisirović, Goran; Silc, Urban

(Gebruder Borntraeger, Stuttgart, 2016)

TY  - JOUR
AU  - Dajić-Stevanović, Zora
AU  - Acić, Svetlana
AU  - Luković, Milica
AU  - Zlatković, Ivana
AU  - Vasin, Jovica
AU  - Topisirović, Goran
AU  - Silc, Urban
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1577
AB  - Aims: A syntaxonomic revision of saline grassland vegetation according to floristic, ecological and biogeographical characteristics. Study area: Southeastern Europe with special emphasis to the Southeastern Balkan. Methods: The set of 2,362 releves from published and unpublished sources originally classified into the classes Thero-Salicornietea, Festuco-Puccinellietea and Molinio-Arrhenatheretea was analysed. Cluster analysis and ordination of the dataset were performed to assess particular vegetation patterns. Diagnostic species of each cluster were determined using the phi-coefficient as a fidelity measure. Results: The analysed dataset could be arranged into four main groups reflecting soil salinity as a key factor for the differentiation of halophytic vegetation. The first group comprised communities of extremely saline and wet soils (Thero-Salicornietea), the second group included highly saline Pannonian grasslands (Puccinellietalia), whereas the third group was rather heterogeneous, grouping different types of saline and alkaline vegetation, mostly on solonetz soil, including vegetation developed on wet saline places, steppe grasslands (Artemisio-Festucetalia) and halophytic vegetation from southern Serbia and R. Macedonia (Puccinellion convolutae). The fourth group comprised sub-Mediterranean grasslands from Molinio-Arrhenatheretea class occurring in habitats of a low salinity level. Conclusions: Variation in different types of halophytic vegetation corresponds to the salinity and moisture gradients. This is an additional attempt of large-scale analysis of the halophytic vegetation focusing on the Southeastern Europe and Balkan in particular. The occurrence of the alliance Puccinelion convolutae was confirmed within saline habitats in southern Serbia. There is an urgent need for conservation of the studied halophytic flora and vegetation.
PB  - Gebruder Borntraeger, Stuttgart
T2  - Phytocoenologia
T1  - Classification of continental halophytic grassland vegetation of Southeastern Europe
EP  - 331
IS  - 3
SP  - 317
VL  - 46
DO  - 10.1127/phyto/2016/0076
ER  - 
@article{
author = "Dajić-Stevanović, Zora and Acić, Svetlana and Luković, Milica and Zlatković, Ivana and Vasin, Jovica and Topisirović, Goran and Silc, Urban",
year = "2016",
abstract = "Aims: A syntaxonomic revision of saline grassland vegetation according to floristic, ecological and biogeographical characteristics. Study area: Southeastern Europe with special emphasis to the Southeastern Balkan. Methods: The set of 2,362 releves from published and unpublished sources originally classified into the classes Thero-Salicornietea, Festuco-Puccinellietea and Molinio-Arrhenatheretea was analysed. Cluster analysis and ordination of the dataset were performed to assess particular vegetation patterns. Diagnostic species of each cluster were determined using the phi-coefficient as a fidelity measure. Results: The analysed dataset could be arranged into four main groups reflecting soil salinity as a key factor for the differentiation of halophytic vegetation. The first group comprised communities of extremely saline and wet soils (Thero-Salicornietea), the second group included highly saline Pannonian grasslands (Puccinellietalia), whereas the third group was rather heterogeneous, grouping different types of saline and alkaline vegetation, mostly on solonetz soil, including vegetation developed on wet saline places, steppe grasslands (Artemisio-Festucetalia) and halophytic vegetation from southern Serbia and R. Macedonia (Puccinellion convolutae). The fourth group comprised sub-Mediterranean grasslands from Molinio-Arrhenatheretea class occurring in habitats of a low salinity level. Conclusions: Variation in different types of halophytic vegetation corresponds to the salinity and moisture gradients. This is an additional attempt of large-scale analysis of the halophytic vegetation focusing on the Southeastern Europe and Balkan in particular. The occurrence of the alliance Puccinelion convolutae was confirmed within saline habitats in southern Serbia. There is an urgent need for conservation of the studied halophytic flora and vegetation.",
publisher = "Gebruder Borntraeger, Stuttgart",
journal = "Phytocoenologia",
title = "Classification of continental halophytic grassland vegetation of Southeastern Europe",
pages = "331-317",
number = "3",
volume = "46",
doi = "10.1127/phyto/2016/0076"
}
Dajić-Stevanović, Z., Acić, S., Luković, M., Zlatković, I., Vasin, J., Topisirović, G.,& Silc, U.. (2016). Classification of continental halophytic grassland vegetation of Southeastern Europe. in Phytocoenologia
Gebruder Borntraeger, Stuttgart., 46(3), 317-331.
https://doi.org/10.1127/phyto/2016/0076
Dajić-Stevanović Z, Acić S, Luković M, Zlatković I, Vasin J, Topisirović G, Silc U. Classification of continental halophytic grassland vegetation of Southeastern Europe. in Phytocoenologia. 2016;46(3):317-331.
doi:10.1127/phyto/2016/0076 .
Dajić-Stevanović, Zora, Acić, Svetlana, Luković, Milica, Zlatković, Ivana, Vasin, Jovica, Topisirović, Goran, Silc, Urban, "Classification of continental halophytic grassland vegetation of Southeastern Europe" in Phytocoenologia, 46, no. 3 (2016):317-331,
https://doi.org/10.1127/phyto/2016/0076 . .
16
9
14

Detection and molecular characterization of pepper mild mottle virus in Serbia

Milošević, Dragana; Stanković, Ivana; Bulajić, Aleksandra; Ignjatov, Maja; Nikolić, Zorica; Petrović, Gordana; Krstić, Branka

(Društvo genetičara Srbije, Beograd, 2015)

TY  - JOUR
AU  - Milošević, Dragana
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Krstić, Branka
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1407
AB  - During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Detection and molecular characterization of pepper mild mottle virus in Serbia
EP  - 663
IS  - 2
SP  - 651
VL  - 47
DO  - 10.2298/GENSR1502651M
ER  - 
@article{
author = "Milošević, Dragana and Stanković, Ivana and Bulajić, Aleksandra and Ignjatov, Maja and Nikolić, Zorica and Petrović, Gordana and Krstić, Branka",
year = "2015",
abstract = "During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Detection and molecular characterization of pepper mild mottle virus in Serbia",
pages = "663-651",
number = "2",
volume = "47",
doi = "10.2298/GENSR1502651M"
}
Milošević, D., Stanković, I., Bulajić, A., Ignjatov, M., Nikolić, Z., Petrović, G.,& Krstić, B.. (2015). Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(2), 651-663.
https://doi.org/10.2298/GENSR1502651M
Milošević D, Stanković I, Bulajić A, Ignjatov M, Nikolić Z, Petrović G, Krstić B. Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika-Belgrade. 2015;47(2):651-663.
doi:10.2298/GENSR1502651M .
Milošević, Dragana, Stanković, Ivana, Bulajić, Aleksandra, Ignjatov, Maja, Nikolić, Zorica, Petrović, Gordana, Krstić, Branka, "Detection and molecular characterization of pepper mild mottle virus in Serbia" in Genetika-Belgrade, 47, no. 2 (2015):651-663,
https://doi.org/10.2298/GENSR1502651M . .
8
2
6

The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia

Milošević, Dragana; Ignjatov, Maja; Nikolić, Zorica; Gvozdanović-Varga, Jelica; Tamindžić, Gordana; Stanković, Ivana; Krstić, Branka

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2015)

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Tamindžić, Gordana
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1477
AB  - During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II.
AB  - Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia
T1  - Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji
EP  - 73
IS  - 2
SP  - 67
VL  - 52
DO  - 10.5937/ratpov52-7814
ER  - 
@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Tamindžić, Gordana and Stanković, Ivana and Krstić, Branka",
year = "2015",
abstract = "During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II., Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia, Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji",
pages = "73-67",
number = "2",
volume = "52",
doi = "10.5937/ratpov52-7814"
}
Milošević, D., Ignjatov, M., Nikolić, Z., Gvozdanović-Varga, J., Tamindžić, G., Stanković, I.,& Krstić, B.. (2015). The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 52(2), 67-73.
https://doi.org/10.5937/ratpov52-7814
Milošević D, Ignjatov M, Nikolić Z, Gvozdanović-Varga J, Tamindžić G, Stanković I, Krstić B. The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2015;52(2):67-73.
doi:10.5937/ratpov52-7814 .
Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Tamindžić, Gordana, Stanković, Ivana, Krstić, Branka, "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 52, no. 2 (2015):67-73,
https://doi.org/10.5937/ratpov52-7814 . .
5