TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3286
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - Basel : MDPI
T2  - Microorganisms - Basel
T1  - Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "Basel : MDPI",
journal = "Microorganisms - Basel",
title = "Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia. in Microorganisms - Basel
Basel : MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Marković S, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia. in Microorganisms - Basel. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia" in Microorganisms - Basel, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3737
AB  - The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - Basel : MDPI
T2  - Microorganisms - Basel
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
SP  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "Basel : MDPI",
journal = "Microorganisms - Basel",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8",
pages = "2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms - Basel
Basel : MDPI., 11(8), 2122.
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms - Basel. 2023;11(8):2122.
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms - Basel, 11, no. 8 (2023):2122,
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Lozo, Jelena
AU  - Danojević, Dario
AU  - Jovanović, Živko
AU  - Nenadović, Željka
AU  - Fira, Đorđe
AU  - Stanković, Slaviša
AU  - Radović, Svetlana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2653
AB  - We examined the effect of drought-tolerant Bacillus safensis SS-2.7 and B. thuringiensis SS-29.2 strains on the response of four (133, 274, California Wonder—CalW, and Matica) sweet pepper genotypes to water deficiency conditions. Pepper seeds were sown in pots with (treated) and without (control) bacterial strain inoculation. After four weeks of growth under controlled conditions and regular watering, drought was imposed by completely withholding watering for seven days. Under conditions of normal watering, genotype 274 showed better seedling establishment than genotype 133 and CalW, while the slowest was genotype Matica. Antioxidant enzyme activity under drought conditions was genotype and bacterial treatment-dependent. The best response to bacterial treatment in order to cope with severe drought was found in the CalW genotype, while in genotype 133, we determined even faster plant decay during water deficiency in treated seeds. Inoculated seeds of the Matica genotype did not show different antioxidant enzyme activity under normal and drought conditions. According to the obtained results, we concluded that under drought conditions, the most susceptible was genotype 274, moderate susceptibility was detected in genotype 133, and CalW and Matica were the most tolerant genotypes. Our study demonstrates (1) that drought-tolerant Bacillus strains showed a plant growth-promoting effect on some selected pepper genotypes; (2) that there were genotype-dependent antioxidant enzyme activities under drought conditions in response to treatment with a particular bacterial strain; and (3) that we could expect a genotype-dependent response during biostimulant application, especially under stress conditions.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains
SP  - 236
VL  - 8
DO  - 10.3390/horticulturae8030236
ER  - 

@article{
author = "Lozo, Jelena and Danojević, Dario and Jovanović, Živko and Nenadović, Željka and Fira, Đorđe and Stanković, Slaviša and Radović, Svetlana",
year = "2022",
abstract = "We examined the effect of drought-tolerant Bacillus safensis SS-2.7 and B. thuringiensis SS-29.2 strains on the response of four (133, 274, California Wonder—CalW, and Matica) sweet pepper genotypes to water deficiency conditions. Pepper seeds were sown in pots with (treated) and without (control) bacterial strain inoculation. After four weeks of growth under controlled conditions and regular watering, drought was imposed by completely withholding watering for seven days. Under conditions of normal watering, genotype 274 showed better seedling establishment than genotype 133 and CalW, while the slowest was genotype Matica. Antioxidant enzyme activity under drought conditions was genotype and bacterial treatment-dependent. The best response to bacterial treatment in order to cope with severe drought was found in the CalW genotype, while in genotype 133, we determined even faster plant decay during water deficiency in treated seeds. Inoculated seeds of the Matica genotype did not show different antioxidant enzyme activity under normal and drought conditions. According to the obtained results, we concluded that under drought conditions, the most susceptible was genotype 274, moderate susceptibility was detected in genotype 133, and CalW and Matica were the most tolerant genotypes. Our study demonstrates (1) that drought-tolerant Bacillus strains showed a plant growth-promoting effect on some selected pepper genotypes; (2) that there were genotype-dependent antioxidant enzyme activities under drought conditions in response to treatment with a particular bacterial strain; and (3) that we could expect a genotype-dependent response during biostimulant application, especially under stress conditions.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains",
pages = "236",
volume = "8",
doi = "10.3390/horticulturae8030236"
}

Lozo, J., Danojević, D., Jovanović, Ž., Nenadović, Ž., Fira, Đ., Stanković, S.,& Radović, S.. (2022). Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains. in Horticulturae
Basel : MDPI., 8, 236.
https://doi.org/10.3390/horticulturae8030236

Lozo J, Danojević D, Jovanović Ž, Nenadović Ž, Fira Đ, Stanković S, Radović S. Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains. in Horticulturae. 2022;8:236.
doi:10.3390/horticulturae8030236 .

Lozo, Jelena, Danojević, Dario, Jovanović, Živko, Nenadović, Željka, Fira, Đorđe, Stanković, Slaviša, Radović, Svetlana, "Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains" in Horticulturae, 8 (2022):236,
https://doi.org/10.3390/horticulturae8030236 . .

TY  - JOUR
AU  - Zeremski, Tijana
AU  - Ranđelović, Dragana
AU  - Jakovljević, Ksenija
AU  - Marjanović-Jeromela, Ana
AU  - Milić, Stanko
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2380
AB  - The genus Brassica is recognized for including species with phytoaccumulation potential and a large amount of research has been carried out in this area under a variety of conditions, from laboratory experiments to field trials, with spiked or naturally contaminated soils, using one or multi-element contaminated soil, generating various and sometimes contradictory results with limited practical applications. To date, the actual field potential of Brassica species and the feasibility of a complete phytoextraction process have not been fully evaluated. Therefore, the aim of this study was to summarize the results of the experiments that have been performed with a view to analyzing real potentials and limitations. The reduced biomass and low metal mobility in the soil have been addressed by the development of chemically or biologically assisted phytoremediation technologies, the use of soil amendments, and the application of crop management strategies. Certain issues, such as the fate of harvested biomass or the performance of species in multi-metal-contaminated soils, remain to be solved by future research. Potential improvements to current experimental settings include testing species grown to full maturity, using a greater amount of soil in experiments, conducting more trials under real field conditions, developing improved crop management systems, and optimizing solutions for harvested biomass disposal.
PB  - Basel : MDPI
T2  - Plants
T1  - Brassica species in phytoextractions: real potentials and challenges
SP  - 2340
VL  - 10
DO  - 10.3390/plants10112340
ER  - 

@article{
author = "Zeremski, Tijana and Ranđelović, Dragana and Jakovljević, Ksenija and Marjanović-Jeromela, Ana and Milić, Stanko",
year = "2021",
abstract = "The genus Brassica is recognized for including species with phytoaccumulation potential and a large amount of research has been carried out in this area under a variety of conditions, from laboratory experiments to field trials, with spiked or naturally contaminated soils, using one or multi-element contaminated soil, generating various and sometimes contradictory results with limited practical applications. To date, the actual field potential of Brassica species and the feasibility of a complete phytoextraction process have not been fully evaluated. Therefore, the aim of this study was to summarize the results of the experiments that have been performed with a view to analyzing real potentials and limitations. The reduced biomass and low metal mobility in the soil have been addressed by the development of chemically or biologically assisted phytoremediation technologies, the use of soil amendments, and the application of crop management strategies. Certain issues, such as the fate of harvested biomass or the performance of species in multi-metal-contaminated soils, remain to be solved by future research. Potential improvements to current experimental settings include testing species grown to full maturity, using a greater amount of soil in experiments, conducting more trials under real field conditions, developing improved crop management systems, and optimizing solutions for harvested biomass disposal.",
publisher = "Basel : MDPI",
journal = "Plants",
title = "Brassica species in phytoextractions: real potentials and challenges",
pages = "2340",
volume = "10",
doi = "10.3390/plants10112340"
}

Zeremski, T., Ranđelović, D., Jakovljević, K., Marjanović-Jeromela, A.,& Milić, S.. (2021). Brassica species in phytoextractions: real potentials and challenges. in Plants
Basel : MDPI., 10, 2340.
https://doi.org/10.3390/plants10112340

Zeremski T, Ranđelović D, Jakovljević K, Marjanović-Jeromela A, Milić S. Brassica species in phytoextractions: real potentials and challenges. in Plants. 2021;10:2340.
doi:10.3390/plants10112340 .

Zeremski, Tijana, Ranđelović, Dragana, Jakovljević, Ksenija, Marjanović-Jeromela, Ana, Milić, Stanko, "Brassica species in phytoextractions: real potentials and challenges" in Plants, 10 (2021):2340,
https://doi.org/10.3390/plants10112340 . .