TY  - JOUR
AU  - Okello, Paul N.
AU  - Petrović, Kristina
AU  - Singh, Asheesh K.
AU  - Kontz, Brian
AU  - Mathew, Febina M.
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1972
AB  - In 2014, 2000 plants showing root rot symptoms were collected from 200 commercial soybean (Glycine max L.) fields in South Dakota, USA. One thousand one hundred thirty fungal isolates obtained from the samples were identified as Fusarium species by morphology and sequencing of the translation elongation factor 1-alpha gene. Fifty-one percent were F. graminearum, 30.0% were F. acuminatum, 8.0% belonged to the F. oxysporum species complex, 3.4% were F. armeniacum, 2.0% were F. proliferatum, 1.6% were F. virguliforme, 1.2% belonged to the F. solani species complex, 0.4% were F. nanum (syn. F. incarnatum-equiseti species complex), 0.1% were F. bulbicola (syn. F. fujikuroi species complex) and 0.1% were F. commune. The pathogenicity of 57 isolates was evaluated on the soybean 'Asgrow 1835' under greenhouse conditions. Root rot severity was rated 14 days after inoculation on a 1-to-5 scale and expressed as relative treatment effects (RTE). Nine isolates of F. oxysporum, and one each of F. armeniacum and F. commune, caused significantly greater RTE than in the non-inoculated control. Twenty-one accessions were screened for resistance in the greenhouse using one isolate each of F. graminearum, F. proliferatum and F. sporotrichioides. Accession PI361090 was less susceptible to F. graminearum and F. proliferatum than the susceptible controls, 'Williams 82' and 'Asgrow 1835'. All accessions were more susceptible to F. sporotrichioides. These findings suggest that 11 Fusarium species or species complexes are pathogenic to soybean in South Dakota, and PI361090 may be a useful source of resistance to F. graminearum and F. proliferatum.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Canadian Journal of Plant Pathology
T1  - Characterization of species of Fusarium causing root rot of Soybean (Glycine max L.) in South Dakota, USA
EP  - 571
IS  - 4
SP  - 560
VL  - 42
DO  - 10.1080/07060661.2020.1746695
ER  - 

@article{
author = "Okello, Paul N. and Petrović, Kristina and Singh, Asheesh K. and Kontz, Brian and Mathew, Febina M.",
year = "2020",
abstract = "In 2014, 2000 plants showing root rot symptoms were collected from 200 commercial soybean (Glycine max L.) fields in South Dakota, USA. One thousand one hundred thirty fungal isolates obtained from the samples were identified as Fusarium species by morphology and sequencing of the translation elongation factor 1-alpha gene. Fifty-one percent were F. graminearum, 30.0% were F. acuminatum, 8.0% belonged to the F. oxysporum species complex, 3.4% were F. armeniacum, 2.0% were F. proliferatum, 1.6% were F. virguliforme, 1.2% belonged to the F. solani species complex, 0.4% were F. nanum (syn. F. incarnatum-equiseti species complex), 0.1% were F. bulbicola (syn. F. fujikuroi species complex) and 0.1% were F. commune. The pathogenicity of 57 isolates was evaluated on the soybean 'Asgrow 1835' under greenhouse conditions. Root rot severity was rated 14 days after inoculation on a 1-to-5 scale and expressed as relative treatment effects (RTE). Nine isolates of F. oxysporum, and one each of F. armeniacum and F. commune, caused significantly greater RTE than in the non-inoculated control. Twenty-one accessions were screened for resistance in the greenhouse using one isolate each of F. graminearum, F. proliferatum and F. sporotrichioides. Accession PI361090 was less susceptible to F. graminearum and F. proliferatum than the susceptible controls, 'Williams 82' and 'Asgrow 1835'. All accessions were more susceptible to F. sporotrichioides. These findings suggest that 11 Fusarium species or species complexes are pathogenic to soybean in South Dakota, and PI361090 may be a useful source of resistance to F. graminearum and F. proliferatum.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Canadian Journal of Plant Pathology",
title = "Characterization of species of Fusarium causing root rot of Soybean (Glycine max L.) in South Dakota, USA",
pages = "571-560",
number = "4",
volume = "42",
doi = "10.1080/07060661.2020.1746695"
}

Okello, P. N., Petrović, K., Singh, A. K., Kontz, B.,& Mathew, F. M.. (2020). Characterization of species of Fusarium causing root rot of Soybean (Glycine max L.) in South Dakota, USA. in Canadian Journal of Plant Pathology
Taylor & Francis Inc, Philadelphia., 42(4), 560-571.
https://doi.org/10.1080/07060661.2020.1746695

Okello PN, Petrović K, Singh AK, Kontz B, Mathew FM. Characterization of species of Fusarium causing root rot of Soybean (Glycine max L.) in South Dakota, USA. in Canadian Journal of Plant Pathology. 2020;42(4):560-571.
doi:10.1080/07060661.2020.1746695 .

Okello, Paul N., Petrović, Kristina, Singh, Asheesh K., Kontz, Brian, Mathew, Febina M., "Characterization of species of Fusarium causing root rot of Soybean (Glycine max L.) in South Dakota, USA" in Canadian Journal of Plant Pathology, 42, no. 4 (2020):560-571,
https://doi.org/10.1080/07060661.2020.1746695 . .

TY  - JOUR
AU  - Ghimire, Krishna
AU  - Petrović, Kristina
AU  - Kontz, Brian
AU  - Bradley, Carl A.
AU  - Chilvers, Martin I.
AU  - Mueller, Daren S.
AU  - Smith, Damon L.
AU  - Wise, Kiersten A.
AU  - Mathew, Febina M.
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1915
AB  - One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P  lt  0.001) and pathogen recovery (P  lt  0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate x method interaction affected disease severity (P  lt  0.05) and pathogen recovery (P  lt  0.001). Significant differences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate x method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)
EP  - 684
IS  - 4
SP  - 677
VL  - 103
DO  - 10.1094/PDIS-06-18-1078-RE
ER  - 

@article{
author = "Ghimire, Krishna and Petrović, Kristina and Kontz, Brian and Bradley, Carl A. and Chilvers, Martin I. and Mueller, Daren S. and Smith, Damon L. and Wise, Kiersten A. and Mathew, Febina M.",
year = "2019",
abstract = "One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P  lt  0.001) and pathogen recovery (P  lt  0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate x method interaction affected disease severity (P  lt  0.05) and pathogen recovery (P  lt  0.001). Significant differences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate x method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)",
pages = "684-677",
number = "4",
volume = "103",
doi = "10.1094/PDIS-06-18-1078-RE"
}

Ghimire, K., Petrović, K., Kontz, B., Bradley, C. A., Chilvers, M. I., Mueller, D. S., Smith, D. L., Wise, K. A.,& Mathew, F. M.. (2019). Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max). in Plant Disease
Amer Phytopathological Soc, St Paul., 103(4), 677-684.
https://doi.org/10.1094/PDIS-06-18-1078-RE

Ghimire K, Petrović K, Kontz B, Bradley CA, Chilvers MI, Mueller DS, Smith DL, Wise KA, Mathew FM. Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max). in Plant Disease. 2019;103(4):677-684.
doi:10.1094/PDIS-06-18-1078-RE .

Ghimire, Krishna, Petrović, Kristina, Kontz, Brian, Bradley, Carl A., Chilvers, Martin I., Mueller, Daren S., Smith, Damon L., Wise, Kiersten A., Mathew, Febina M., "Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)" in Plant Disease, 103, no. 4 (2019):677-684,
https://doi.org/10.1094/PDIS-06-18-1078-RE . .