TY  - CONF
AU  - Radanović, Aleksandra
AU  - Horn, Renate
AU  - Sprycha, Yves
AU  - Hamrit, Sonia
AU  - Jocković, Milan
AU  - Cvejić, Sandra
AU  - Jocić, Siniša
AU  - Miklič, Vladimir
AU  - Miladinović, Dragana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3063
AB  - Sunflower is the second most common crop among hybrids, worldwide. Development of cytoplasmic male sterile and fertility restoration lines enables creation of hybrids. Sunflower hybrid breeding most frequently relies on the combination of the CMS PET1 cytoplasm and the fertility restoration gene Rf1. Use of molecular markers can accelerate creation of restorer lines. Previous work on this subject included mapping of the Rf1 gene on chromosome 13 and development of bacterial artificial chromosome (BAC) libraries for the restorer line RHA325 and the maintainer line HA383, which enabled positioning of BAC clones surrounding the Rf1 gene in the cross RHA325 x HA342. In this study, BAC-end sequences were used to derive primers in order to amplify selected regions from RHA325 and the maintainer line, HA342. While the majority of primer combinations were monomorphic, some were polymorphic between RHA325 and HA342. Previously reported markers for detection of Rf1 gene and newly developed ones based on BAC-end sequences were further tested on hybrids and its components (A, B and R lines) created at the Institute of Field and Vegetable Crops, Novi Sad.
PB  - Belgrade : Serbian Genetic Society
C3  - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
T1  - Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower
EP  - 186
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3063
ER  - 

@conference{
author = "Radanović, Aleksandra and Horn, Renate and Sprycha, Yves and Hamrit, Sonia and Jocković, Milan and Cvejić, Sandra and Jocić, Siniša and Miklič, Vladimir and Miladinović, Dragana",
year = "2019",
abstract = "Sunflower is the second most common crop among hybrids, worldwide. Development of cytoplasmic male sterile and fertility restoration lines enables creation of hybrids. Sunflower hybrid breeding most frequently relies on the combination of the CMS PET1 cytoplasm and the fertility restoration gene Rf1. Use of molecular markers can accelerate creation of restorer lines. Previous work on this subject included mapping of the Rf1 gene on chromosome 13 and development of bacterial artificial chromosome (BAC) libraries for the restorer line RHA325 and the maintainer line HA383, which enabled positioning of BAC clones surrounding the Rf1 gene in the cross RHA325 x HA342. In this study, BAC-end sequences were used to derive primers in order to amplify selected regions from RHA325 and the maintainer line, HA342. While the majority of primer combinations were monomorphic, some were polymorphic between RHA325 and HA342. Previously reported markers for detection of Rf1 gene and newly developed ones based on BAC-end sequences were further tested on hybrids and its components (A, B and R lines) created at the Institute of Field and Vegetable Crops, Novi Sad.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja",
title = "Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower",
pages = "186-186",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3063"
}

Radanović, A., Horn, R., Sprycha, Y., Hamrit, S., Jocković, M., Cvejić, S., Jocić, S., Miklič, V.,& Miladinović, D.. (2019). Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
Belgrade : Serbian Genetic Society., 186-186.
https://hdl.handle.net/21.15107/rcub_fiver_3063

Radanović A, Horn R, Sprycha Y, Hamrit S, Jocković M, Cvejić S, Jocić S, Miklič V, Miladinović D. Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:186-186.
https://hdl.handle.net/21.15107/rcub_fiver_3063 .

Radanović, Aleksandra, Horn, Renate, Sprycha, Yves, Hamrit, Sonia, Jocković, Milan, Cvejić, Sandra, Jocić, Siniša, Miklič, Vladimir, Miladinović, Dragana, "Molecular markers for detection of Rf1 gene developed from BAC-end sequences in sunflower" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):186-186,
https://hdl.handle.net/21.15107/rcub_fiver_3063 .