APV 142-451-3213/2020-03 financed by the Provincial Secretariat for Higher Education and Scientific Research, AP Vojvodina

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APV 142-451-3213/2020-03 financed by the Provincial Secretariat for Higher Education and Scientific Research, AP Vojvodina

Authors

Publications

Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development

Mitrović, Ivana; Vučurović, Damjan; Al-Ani, Laith Khalil Tawfeeq; Mitrović, Bojan; Bajić, Bojana; Dodić, Siniša; Tančić-Živanov, Sonja

(Oxford : Oxford University Press, 2023)

TY  - JOUR
AU  - Mitrović, Ivana
AU  - Vučurović, Damjan
AU  - Al-Ani, Laith Khalil Tawfeeq
AU  - Mitrović, Bojan
AU  - Bajić, Bojana
AU  - Dodić, Siniša
AU  - Tančić-Živanov, Sonja
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4532
AB  - In order to be competitive on the market, the production of biopreparations needs to be optimized, modelled, and assessed in the early stages of its development. The aim of this paper was to optimize medium for the production of Trichoderma harzianum K179 biocontrol agent, to analyze its kinetics at enlarged laboratory scale and finally economic analysis of the production of this high-value product through simulation modelling. The results showed that the bioprocess of T. harzianum K179 bioagent production in a laboratory bioreactor on the medium with optimal composition (dextrose 10 g l−1, soy flour 6.87 g l−1, K2HPO4 1.51 g l−1, KCl 0.5 g l−1, and MgSO4 × 7H2O 0.5 g l−1), at stirring speed of 1.75 × g and aeration intensity of 1.5 vvm, can be shortened from 96 to 36 h. The results of bioprocess economic analysis showed that with a 25-year project lifetime and an investment payback time of 7.58 years, this project represents an economically viable system.
PB  - Oxford : Oxford University Press
T2  - Journal of Applied Microbiology
T1  - Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development
IS  - 6
SP  - lxad115
VL  - 134
DO  - 10.1093/jambio/lxad115
ER  - 
@article{
author = "Mitrović, Ivana and Vučurović, Damjan and Al-Ani, Laith Khalil Tawfeeq and Mitrović, Bojan and Bajić, Bojana and Dodić, Siniša and Tančić-Živanov, Sonja",
year = "2023",
abstract = "In order to be competitive on the market, the production of biopreparations needs to be optimized, modelled, and assessed in the early stages of its development. The aim of this paper was to optimize medium for the production of Trichoderma harzianum K179 biocontrol agent, to analyze its kinetics at enlarged laboratory scale and finally economic analysis of the production of this high-value product through simulation modelling. The results showed that the bioprocess of T. harzianum K179 bioagent production in a laboratory bioreactor on the medium with optimal composition (dextrose 10 g l−1, soy flour 6.87 g l−1, K2HPO4 1.51 g l−1, KCl 0.5 g l−1, and MgSO4 × 7H2O 0.5 g l−1), at stirring speed of 1.75 × g and aeration intensity of 1.5 vvm, can be shortened from 96 to 36 h. The results of bioprocess economic analysis showed that with a 25-year project lifetime and an investment payback time of 7.58 years, this project represents an economically viable system.",
publisher = "Oxford : Oxford University Press",
journal = "Journal of Applied Microbiology",
title = "Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development",
number = "6",
pages = "lxad115",
volume = "134",
doi = "10.1093/jambio/lxad115"
}
Mitrović, I., Vučurović, D., Al-Ani, L. K. T., Mitrović, B., Bajić, B., Dodić, S.,& Tančić-Živanov, S.. (2023). Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development. in Journal of Applied Microbiology
Oxford : Oxford University Press., 134(6), lxad115.
https://doi.org/10.1093/jambio/lxad115
Mitrović I, Vučurović D, Al-Ani LKT, Mitrović B, Bajić B, Dodić S, Tančić-Živanov S. Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development. in Journal of Applied Microbiology. 2023;134(6):lxad115.
doi:10.1093/jambio/lxad115 .
Mitrović, Ivana, Vučurović, Damjan, Al-Ani, Laith Khalil Tawfeeq, Mitrović, Bojan, Bajić, Bojana, Dodić, Siniša, Tančić-Živanov, Sonja, "Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development" in Journal of Applied Microbiology, 134, no. 6 (2023):lxad115,
https://doi.org/10.1093/jambio/lxad115 . .

Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development

Mitrović, Ivana; Vučurović, Damjan; Al-Ani, Laith Khalil Tawfeeq; Mitrović, Bojan; Bajić, Bojana; Dodić, Siniša; Tančić-Živanov, Sonja

(Oxford : Oxford University Press, 2023)

TY  - JOUR
AU  - Mitrović, Ivana
AU  - Vučurović, Damjan
AU  - Al-Ani, Laith Khalil Tawfeeq
AU  - Mitrović, Bojan
AU  - Bajić, Bojana
AU  - Dodić, Siniša
AU  - Tančić-Živanov, Sonja
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3979
AB  - In order to be competitive on the market, the production of biopreparations needs to be optimized, modelled, and assessed in the early stages of its development. The aim of this paper was to optimize medium for the production of Trichoderma harzianum K179 biocontrol agent, to analyze its kinetics at enlarged laboratory scale and finally economic analysis of the production of this high-value product through simulation modelling. The results showed that the bioprocess of T. harzianum K179 bioagent production in a laboratory bioreactor on the medium with optimal composition (dextrose 10 g l−1, soy flour 6.87 g l−1, K2HPO4 1.51 g l−1, KCl 0.5 g l−1, and MgSO4 × 7H2O 0.5 g l−1), at stirring speed of 1.75 × g and aeration intensity of 1.5 vvm, can be shortened from 96 to 36 h. The results of bioprocess economic analysis showed that with a 25-year project lifetime and an investment payback time of 7.58 years, this project represents an economically viable system.
PB  - Oxford : Oxford University Press
T2  - Journal of Applied Microbiology
T1  - Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development
IS  - 6
SP  - lxad115
VL  - 134
DO  - 10.1093/jambio/lxad115
ER  - 
@article{
author = "Mitrović, Ivana and Vučurović, Damjan and Al-Ani, Laith Khalil Tawfeeq and Mitrović, Bojan and Bajić, Bojana and Dodić, Siniša and Tančić-Živanov, Sonja",
year = "2023",
abstract = "In order to be competitive on the market, the production of biopreparations needs to be optimized, modelled, and assessed in the early stages of its development. The aim of this paper was to optimize medium for the production of Trichoderma harzianum K179 biocontrol agent, to analyze its kinetics at enlarged laboratory scale and finally economic analysis of the production of this high-value product through simulation modelling. The results showed that the bioprocess of T. harzianum K179 bioagent production in a laboratory bioreactor on the medium with optimal composition (dextrose 10 g l−1, soy flour 6.87 g l−1, K2HPO4 1.51 g l−1, KCl 0.5 g l−1, and MgSO4 × 7H2O 0.5 g l−1), at stirring speed of 1.75 × g and aeration intensity of 1.5 vvm, can be shortened from 96 to 36 h. The results of bioprocess economic analysis showed that with a 25-year project lifetime and an investment payback time of 7.58 years, this project represents an economically viable system.",
publisher = "Oxford : Oxford University Press",
journal = "Journal of Applied Microbiology",
title = "Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development",
number = "6",
pages = "lxad115",
volume = "134",
doi = "10.1093/jambio/lxad115"
}
Mitrović, I., Vučurović, D., Al-Ani, L. K. T., Mitrović, B., Bajić, B., Dodić, S.,& Tančić-Živanov, S.. (2023). Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development. in Journal of Applied Microbiology
Oxford : Oxford University Press., 134(6), lxad115.
https://doi.org/10.1093/jambio/lxad115
Mitrović I, Vučurović D, Al-Ani LKT, Mitrović B, Bajić B, Dodić S, Tančić-Živanov S. Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development. in Journal of Applied Microbiology. 2023;134(6):lxad115.
doi:10.1093/jambio/lxad115 .
Mitrović, Ivana, Vučurović, Damjan, Al-Ani, Laith Khalil Tawfeeq, Mitrović, Bojan, Bajić, Bojana, Dodić, Siniša, Tančić-Živanov, Sonja, "Production of Trichoderma harzianum K179 bioagent for maize diseases control: complete laboratory stage bioprocess development" in Journal of Applied Microbiology, 134, no. 6 (2023):lxad115,
https://doi.org/10.1093/jambio/lxad115 . .

Influence of process parameters on the production of Trichoderma biocontrol agent

Mitrović, Ivana; Tančić-Živanov, Sonja; Purar, Božana; Trivunović, Zorana; Mitrović, Bojan

(Tuzla: Univerzitet u Tuzli, Tehnološki fakultet, 2021)

TY  - CONF
AU  - Mitrović, Ivana
AU  - Tančić-Živanov, Sonja
AU  - Purar, Božana
AU  - Trivunović, Zorana
AU  - Mitrović, Bojan
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2530
AB  - Maize is an agricultural crop that is susceptible to infections by various phytopathogenic fungi, producers of mycotoxins harmful to humans and animals. Since this agricultural crop has an important place in the human diet, its health safety is very important. Trichoderma genus has great potential in the biocontrol of various phytopathogens however, the medium composition as well as the cultivation conditions, have a significant impact on the efficiency of the produced Trichoderma bioagents. In this work, influence of medium pH, temperature and mixing speed on the productivity of Trichoderma bioagent effective against two maize pathogens, Fusarium graminearum and Aspergillus flavus, was investigated. The results obtained by statistical processing show that the best productivity of Trichoderma harzianum bioagent was achieved when the pH of the medium was 6, the temperature was 28°C and the mixing speed of the rotary shaker was 180 rpm. By applying these cultivation conditions, the largest inhibition zone diameters of F. graminearum and A. flavus mycelial growth were formed. Also, the results show that the maize pathogen, F. graminearum, was more sensitive to the produced Trichoderma biocontrol agent compared to the other maize pathogen, A. flavus.
PB  - Tuzla: Univerzitet u Tuzli, Tehnološki fakultet
C3  - Book of Abstracts, OPORPH 2021, 7th International scientific-professional symposium “Environmental resources, sustainable development and food production”,12 November 2021, Tuzla, Bosnia and Herzegovina
T1  - Influence of process parameters on the production of Trichoderma biocontrol agent
EP  - 8
VL  - 8
DO  - 10.5281/zenodo.5656338
ER  - 
@conference{
author = "Mitrović, Ivana and Tančić-Živanov, Sonja and Purar, Božana and Trivunović, Zorana and Mitrović, Bojan",
year = "2021",
abstract = "Maize is an agricultural crop that is susceptible to infections by various phytopathogenic fungi, producers of mycotoxins harmful to humans and animals. Since this agricultural crop has an important place in the human diet, its health safety is very important. Trichoderma genus has great potential in the biocontrol of various phytopathogens however, the medium composition as well as the cultivation conditions, have a significant impact on the efficiency of the produced Trichoderma bioagents. In this work, influence of medium pH, temperature and mixing speed on the productivity of Trichoderma bioagent effective against two maize pathogens, Fusarium graminearum and Aspergillus flavus, was investigated. The results obtained by statistical processing show that the best productivity of Trichoderma harzianum bioagent was achieved when the pH of the medium was 6, the temperature was 28°C and the mixing speed of the rotary shaker was 180 rpm. By applying these cultivation conditions, the largest inhibition zone diameters of F. graminearum and A. flavus mycelial growth were formed. Also, the results show that the maize pathogen, F. graminearum, was more sensitive to the produced Trichoderma biocontrol agent compared to the other maize pathogen, A. flavus.",
publisher = "Tuzla: Univerzitet u Tuzli, Tehnološki fakultet",
journal = "Book of Abstracts, OPORPH 2021, 7th International scientific-professional symposium “Environmental resources, sustainable development and food production”,12 November 2021, Tuzla, Bosnia and Herzegovina",
title = "Influence of process parameters on the production of Trichoderma biocontrol agent",
pages = "8",
volume = "8",
doi = "10.5281/zenodo.5656338"
}
Mitrović, I., Tančić-Živanov, S., Purar, B., Trivunović, Z.,& Mitrović, B.. (2021). Influence of process parameters on the production of Trichoderma biocontrol agent. in Book of Abstracts, OPORPH 2021, 7th International scientific-professional symposium “Environmental resources, sustainable development and food production”,12 November 2021, Tuzla, Bosnia and Herzegovina
Tuzla: Univerzitet u Tuzli, Tehnološki fakultet., 8.
https://doi.org/10.5281/zenodo.5656338
Mitrović I, Tančić-Živanov S, Purar B, Trivunović Z, Mitrović B. Influence of process parameters on the production of Trichoderma biocontrol agent. in Book of Abstracts, OPORPH 2021, 7th International scientific-professional symposium “Environmental resources, sustainable development and food production”,12 November 2021, Tuzla, Bosnia and Herzegovina. 2021;8:null-8.
doi:10.5281/zenodo.5656338 .
Mitrović, Ivana, Tančić-Živanov, Sonja, Purar, Božana, Trivunović, Zorana, Mitrović, Bojan, "Influence of process parameters on the production of Trichoderma biocontrol agent" in Book of Abstracts, OPORPH 2021, 7th International scientific-professional symposium “Environmental resources, sustainable development and food production”,12 November 2021, Tuzla, Bosnia and Herzegovina, 8 (2021),
https://doi.org/10.5281/zenodo.5656338 . .

Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum

Mitrović, Ivana; Tančić-Živanov, Sonja; Purar, Božana; Trivunović, Zorana; Mitrović, Bojan

(Novi Sad: Institut za ratarstvo i povrtarstvo, 2021)

TY  - JOUR
AU  - Mitrović, Ivana
AU  - Tančić-Živanov, Sonja
AU  - Purar, Božana
AU  - Trivunović, Zorana
AU  - Mitrović, Bojan
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2527
AB  - The increasing usage of chemicals for plant protection in recent years has become a serious problem. One of the possible solutions is use of beneficial microorganisms instead of synthetic fungicides, which will contribute to the protection of the environment and human health. Since the fungi Aspergillus flavus and Fusarium graminearum are the most important pathogens that cause maize diseases and produce mycotoxins, the potential of Trichoderma harzianum for biocontrol of both phytopathogens was examined in this paper. The aim of this paper was to study the influence of different carbon and nitrogen combinations in the medium for T. harzianum production. T. harzianum was cultivated in Erlenmeyer flasks and the effect of cultivation broth against selected maize pathogens was tested using well diffusion method. The results of this study showed that the combination of different carbon and nitrogen sources in the T. harzianum cultivation medium statistically significantly affects the production of Trichoderma cultivation broth effective on two tested phytopathogens. Dextrose as a carbon source and soybean flour as a nitrogen source proved to be the best combination in the medium for production of T. harzianum cultivation broth effective on A. flavus and F. graminearum. Maximal inhibition zone diameters of 31 mm and 56.33 mm were registered in those medium formulations for A. flavus and F. graminearum, respectively. These researches represent an important step for further research in which a medium of low market value would be selected. This would reduce the price of the production process but also the final product.
AB  - Sve veća upotreba hemijskih sredstava za zaštitu bilja poslednjih godina postala je ozbiljan problem. Upotreba korisnih mikroorganizama umesto sintetičkih fungicida u biološkoj zaštiti sigurno je jedno od rešenja koje doprinose zaštiti životne sredine i ljudskog zdravlja. S obzirom na to da se gljive Aspergillus flavus i Fusarium graminearum pominju kao najvažniji prouzrokovači bolesti kukuruza, proizvođači mikotoksina, u ovom radu ispitan je antifungalni efekat Trichoderma harzianum na obe fitopatogene gljive. Cilj ovog rada je ispitati uticaj različitih kombinacija ugljenika i azota u podlozi za proizvodnju T. harzianum. Kultivacija T. harzianum izvedena je u erlenmajerima na rotacionoj tresilici, a aktivnost dobijene kultivacione tečnosti na odabrane fitopatogene kukuruza ispitana je difuzionom metodom sa bunarima. Rezultati ovog rada pokazali su da kombinacija različitih izvora ugljenika i azota u podlozi statistički značajno utiče na proizvodnju Trichoderma kultivacione tečnosti efikasne na dve testirane fitopatogene gljive. Kombinacija koja sadrži dekstrozu kao izvor ugljenika i sojino brašno kao izvor azota pokazala se najboljom za formulaciju podloge za proizvodnju T. harzianum kultivacione tečnosti efikasne na patogene kukuruza, A. flavus i F. graminearum, formirajući maksimalne prečnike zona inhibicije od 31 mm, odnosno 56,33 mm.
PB  - Novi Sad: Institut za ratarstvo i povrtarstvo
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum
T1  - Uticaj različitih kombinacija izvora ugljenika i azota u podlozi za proizvodnju biokontrolnog agensa Trichoderma harzianum
EP  - 6
IS  - 1
SP  - 1
VL  - 58
DO  - 10.5937/ratpov58-29961
ER  - 
@article{
author = "Mitrović, Ivana and Tančić-Živanov, Sonja and Purar, Božana and Trivunović, Zorana and Mitrović, Bojan",
year = "2021",
abstract = "The increasing usage of chemicals for plant protection in recent years has become a serious problem. One of the possible solutions is use of beneficial microorganisms instead of synthetic fungicides, which will contribute to the protection of the environment and human health. Since the fungi Aspergillus flavus and Fusarium graminearum are the most important pathogens that cause maize diseases and produce mycotoxins, the potential of Trichoderma harzianum for biocontrol of both phytopathogens was examined in this paper. The aim of this paper was to study the influence of different carbon and nitrogen combinations in the medium for T. harzianum production. T. harzianum was cultivated in Erlenmeyer flasks and the effect of cultivation broth against selected maize pathogens was tested using well diffusion method. The results of this study showed that the combination of different carbon and nitrogen sources in the T. harzianum cultivation medium statistically significantly affects the production of Trichoderma cultivation broth effective on two tested phytopathogens. Dextrose as a carbon source and soybean flour as a nitrogen source proved to be the best combination in the medium for production of T. harzianum cultivation broth effective on A. flavus and F. graminearum. Maximal inhibition zone diameters of 31 mm and 56.33 mm were registered in those medium formulations for A. flavus and F. graminearum, respectively. These researches represent an important step for further research in which a medium of low market value would be selected. This would reduce the price of the production process but also the final product., Sve veća upotreba hemijskih sredstava za zaštitu bilja poslednjih godina postala je ozbiljan problem. Upotreba korisnih mikroorganizama umesto sintetičkih fungicida u biološkoj zaštiti sigurno je jedno od rešenja koje doprinose zaštiti životne sredine i ljudskog zdravlja. S obzirom na to da se gljive Aspergillus flavus i Fusarium graminearum pominju kao najvažniji prouzrokovači bolesti kukuruza, proizvođači mikotoksina, u ovom radu ispitan je antifungalni efekat Trichoderma harzianum na obe fitopatogene gljive. Cilj ovog rada je ispitati uticaj različitih kombinacija ugljenika i azota u podlozi za proizvodnju T. harzianum. Kultivacija T. harzianum izvedena je u erlenmajerima na rotacionoj tresilici, a aktivnost dobijene kultivacione tečnosti na odabrane fitopatogene kukuruza ispitana je difuzionom metodom sa bunarima. Rezultati ovog rada pokazali su da kombinacija različitih izvora ugljenika i azota u podlozi statistički značajno utiče na proizvodnju Trichoderma kultivacione tečnosti efikasne na dve testirane fitopatogene gljive. Kombinacija koja sadrži dekstrozu kao izvor ugljenika i sojino brašno kao izvor azota pokazala se najboljom za formulaciju podloge za proizvodnju T. harzianum kultivacione tečnosti efikasne na patogene kukuruza, A. flavus i F. graminearum, formirajući maksimalne prečnike zona inhibicije od 31 mm, odnosno 56,33 mm.",
publisher = "Novi Sad: Institut za ratarstvo i povrtarstvo",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum, Uticaj različitih kombinacija izvora ugljenika i azota u podlozi za proizvodnju biokontrolnog agensa Trichoderma harzianum",
pages = "6-1",
number = "1",
volume = "58",
doi = "10.5937/ratpov58-29961"
}
Mitrović, I., Tančić-Živanov, S., Purar, B., Trivunović, Z.,& Mitrović, B.. (2021). Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Novi Sad: Institut za ratarstvo i povrtarstvo., 58(1), 1-6.
https://doi.org/10.5937/ratpov58-29961
Mitrović I, Tančić-Živanov S, Purar B, Trivunović Z, Mitrović B. Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2021;58(1):1-6.
doi:10.5937/ratpov58-29961 .
Mitrović, Ivana, Tančić-Živanov, Sonja, Purar, Božana, Trivunović, Zorana, Mitrović, Bojan, "Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 58, no. 1 (2021):1-6,
https://doi.org/10.5937/ratpov58-29961 . .
3
3

Biocontrol potential of different trichoderma isolates against maize phytopathogens

Mitrović, Ivana; Tančić-Živanov, Sonja; Purar, Božana; Mitrović, Bojan

(Zvornik: Fakultet za tehnologiju, 2021)

TY  - CONF
AU  - Mitrović, Ivana
AU  - Tančić-Živanov, Sonja
AU  - Purar, Božana
AU  - Mitrović, Bojan
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2539
AB  - Usage of chemical plant protection agents in agricultural production contributes to environmental pollution and emissions of factors harmful for human health. It has been noted that their overexploitation has resulted in the emergence of resistant species and chemical compounds in the food chain, which indicates the need to find new agents with improved characteristics. One of the solutions is certainly the use of beneficial microorganisms. Considering that maize is a significant agricultural crop very present in the human diet, its health safety is of great importance. In this paper, the ability of three Trichoderma isolates, isolated from the environment, in the biological control of the most common maize pathogens, was examined. Cultivation of Tricoderma isolates was performed on PDB (Potato Dextrose Broth) medium by shaking at 150 rpm for 7 days at 25°C. After 7 days of cultivation, the effect of both, cultivation broth and supernatant, against selected maize phytopathogens was examined using wells diffusion technique. The results showed that the cultivation broth showed a statistically significant efficacy on selected maize phytopathogens compared to the supernatant, using all Trichoderma isolates. By applying the Scheffe test, it was determined that the best effect on the test maize phytopathogens show cultivation broth of T. harzianum, forming maximum mean inhibition zone diameters of 50.33 mm for Fusarium graminearum, 40.67 mm for Helminthosporium carbonum, 25.67 mm for Aspergillus flavus and 25.33 mm for Penicillium sp. Since each inhibition zone larger than 22 mm shows that the produced agent is highly efficient, based on the obtained inhibition zone diameters of all tested isolates, it can be concluded that the produced T. harzianum cultivation broth shows high efficacy against four maize phytopathogens in vitro. This results once again confirms the great potential of Trichoderma isolates in biological control, which certainly contributes to the development of sustainable agricultural production and consequently has a positive impact on human health.
PB  - Zvornik: Fakultet za tehnologiju
C3  - Proceedings, 7th International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, BiH, 17-19 March 2021
T1  - Biocontrol potential of different trichoderma isolates against maize phytopathogens
EP  - 69
SP  - 62
DO  - 10.7251/EEMEN2101062M
ER  - 
@conference{
author = "Mitrović, Ivana and Tančić-Živanov, Sonja and Purar, Božana and Mitrović, Bojan",
year = "2021",
abstract = "Usage of chemical plant protection agents in agricultural production contributes to environmental pollution and emissions of factors harmful for human health. It has been noted that their overexploitation has resulted in the emergence of resistant species and chemical compounds in the food chain, which indicates the need to find new agents with improved characteristics. One of the solutions is certainly the use of beneficial microorganisms. Considering that maize is a significant agricultural crop very present in the human diet, its health safety is of great importance. In this paper, the ability of three Trichoderma isolates, isolated from the environment, in the biological control of the most common maize pathogens, was examined. Cultivation of Tricoderma isolates was performed on PDB (Potato Dextrose Broth) medium by shaking at 150 rpm for 7 days at 25°C. After 7 days of cultivation, the effect of both, cultivation broth and supernatant, against selected maize phytopathogens was examined using wells diffusion technique. The results showed that the cultivation broth showed a statistically significant efficacy on selected maize phytopathogens compared to the supernatant, using all Trichoderma isolates. By applying the Scheffe test, it was determined that the best effect on the test maize phytopathogens show cultivation broth of T. harzianum, forming maximum mean inhibition zone diameters of 50.33 mm for Fusarium graminearum, 40.67 mm for Helminthosporium carbonum, 25.67 mm for Aspergillus flavus and 25.33 mm for Penicillium sp. Since each inhibition zone larger than 22 mm shows that the produced agent is highly efficient, based on the obtained inhibition zone diameters of all tested isolates, it can be concluded that the produced T. harzianum cultivation broth shows high efficacy against four maize phytopathogens in vitro. This results once again confirms the great potential of Trichoderma isolates in biological control, which certainly contributes to the development of sustainable agricultural production and consequently has a positive impact on human health.",
publisher = "Zvornik: Fakultet za tehnologiju",
journal = "Proceedings, 7th International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, BiH, 17-19 March 2021",
title = "Biocontrol potential of different trichoderma isolates against maize phytopathogens",
pages = "69-62",
doi = "10.7251/EEMEN2101062M"
}
Mitrović, I., Tančić-Živanov, S., Purar, B.,& Mitrović, B.. (2021). Biocontrol potential of different trichoderma isolates against maize phytopathogens. in Proceedings, 7th International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, BiH, 17-19 March 2021
Zvornik: Fakultet za tehnologiju., 62-69.
https://doi.org/10.7251/EEMEN2101062M
Mitrović I, Tančić-Živanov S, Purar B, Mitrović B. Biocontrol potential of different trichoderma isolates against maize phytopathogens. in Proceedings, 7th International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, BiH, 17-19 March 2021. 2021;:62-69.
doi:10.7251/EEMEN2101062M .
Mitrović, Ivana, Tančić-Živanov, Sonja, Purar, Božana, Mitrović, Bojan, "Biocontrol potential of different trichoderma isolates against maize phytopathogens" in Proceedings, 7th International Congress “Engineering, Environment and Materials in Process Industry“, Jahorina, BiH, 17-19 March 2021 (2021):62-69,
https://doi.org/10.7251/EEMEN2101062M . .