TY  - JOUR
AU  - Radovanović, Katarina
AU  - Gavarić, Neda
AU  - Švarc-Gajić, Jaroslava
AU  - Brezo-Borjan, Tanja
AU  - Zlatković, Bojan
AU  - Lončar, Biljana
AU  - Aćimović, Milica
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3342
AB  - The genus Achillea has significant medical potential due to the presence of highly bioactive compounds in its chemical composition. To take advantage of plants’ biomedical potential, it is of great importance to use a proper extraction process. This study aimed to determine and compare the preliminary chemical composition of five different Achillea species extracted with two conventional (infusion and maceration) and two non-conventional (ultrasound-assisted extraction (UAE) and Subcritical water extraction (SWE)) techniques. The extracts were prepared using the previously described procedures for infusion, maceration, UAE and SWE extraction. For all extracts, the extraction yield (dry extract (DE)) was determined. The analyzed extracts were preliminarily chemically characterized spectrophotometrically in terms of total phenolic content (TPC) and total flavonoid content (TFC). The obtained results showed that non-conventional techniques delivered higher values of TPC and TFC than conventional. There is a statistically significant increase in DE and TPC content when applying SWE for all observed Achillea species. The highest DE value, 48.80 ± 1.76%, was observed for A. asplenifolia. The highest TPC values were observed after applying SWE: 93.63 ± 1.01 mg GAE/g DE for A. millefolium, and 90.12 ± 0.87 mg GAE/g DE for A. crithmifolia. The results for TFC revealed a statistically significant difference in values, with A. nobilis subsp. nelreichii as the sample with the highest content of TFC (11.11 ± 0.22 mg QE/g DE) when using UAE. Consequently, it could be concluded that SWE is a superior non-conventional extraction technique, and A. nobilis subsp. nerleichii presents as the most promising plant.
PB  - Basel : MDPI
T2  - Processes - Basel
T1  - Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species
IS  - 1
SP  - 86
VL  - 11
DO  - 10.3390/pr11010086
ER  - 

@article{
author = "Radovanović, Katarina and Gavarić, Neda and Švarc-Gajić, Jaroslava and Brezo-Borjan, Tanja and Zlatković, Bojan and Lončar, Biljana and Aćimović, Milica",
year = "2023",
abstract = "The genus Achillea has significant medical potential due to the presence of highly bioactive compounds in its chemical composition. To take advantage of plants’ biomedical potential, it is of great importance to use a proper extraction process. This study aimed to determine and compare the preliminary chemical composition of five different Achillea species extracted with two conventional (infusion and maceration) and two non-conventional (ultrasound-assisted extraction (UAE) and Subcritical water extraction (SWE)) techniques. The extracts were prepared using the previously described procedures for infusion, maceration, UAE and SWE extraction. For all extracts, the extraction yield (dry extract (DE)) was determined. The analyzed extracts were preliminarily chemically characterized spectrophotometrically in terms of total phenolic content (TPC) and total flavonoid content (TFC). The obtained results showed that non-conventional techniques delivered higher values of TPC and TFC than conventional. There is a statistically significant increase in DE and TPC content when applying SWE for all observed Achillea species. The highest DE value, 48.80 ± 1.76%, was observed for A. asplenifolia. The highest TPC values were observed after applying SWE: 93.63 ± 1.01 mg GAE/g DE for A. millefolium, and 90.12 ± 0.87 mg GAE/g DE for A. crithmifolia. The results for TFC revealed a statistically significant difference in values, with A. nobilis subsp. nelreichii as the sample with the highest content of TFC (11.11 ± 0.22 mg QE/g DE) when using UAE. Consequently, it could be concluded that SWE is a superior non-conventional extraction technique, and A. nobilis subsp. nerleichii presents as the most promising plant.",
publisher = "Basel : MDPI",
journal = "Processes - Basel",
title = "Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species",
number = "1",
pages = "86",
volume = "11",
doi = "10.3390/pr11010086"
}

Radovanović, K., Gavarić, N., Švarc-Gajić, J., Brezo-Borjan, T., Zlatković, B., Lončar, B.,& Aćimović, M.. (2023). Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species. in Processes - Basel
Basel : MDPI., 11(1), 86.
https://doi.org/10.3390/pr11010086

Radovanović K, Gavarić N, Švarc-Gajić J, Brezo-Borjan T, Zlatković B, Lončar B, Aćimović M. Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species. in Processes - Basel. 2023;11(1):86.
doi:10.3390/pr11010086 .

Radovanović, Katarina, Gavarić, Neda, Švarc-Gajić, Jaroslava, Brezo-Borjan, Tanja, Zlatković, Bojan, Lončar, Biljana, Aćimović, Milica, "Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species" in Processes - Basel, 11, no. 1 (2023):86,
https://doi.org/10.3390/pr11010086 . .

TY  - JOUR
AU  - Mikić, Aleksandar
AU  - Smykal, Petr
AU  - Kenicer, Gregory
AU  - Vishnyakova, Margarita
AU  - Sarukhanyan, Nune
AU  - Akopian, Janna
AU  - Vanyan, Armen
AU  - Gabrielyan, Ivan
AU  - Smykalova, Iva
AU  - Sherbakova, Ekaterina
AU  - Zorić, Lana
AU  - Atlagić, Jovanka
AU  - Zeremski-Škorić, Tijana
AU  - Ćupina, Branko
AU  - Krstić, Đorđe
AU  - Jajić, Igor
AU  - Antanasović, Svetlana
AU  - Đorđević, Vuk
AU  - Mihailović, Vojislav
AU  - Ivanov, Alexandr
AU  - Ochatt, Sergio
AU  - Toker, Cengiz
AU  - Zlatković, Bojan
AU  - Ambrose, Mike
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1329
AB  - Main conclusion Vavilovia formosa (Stev.) Fed. is a scientifically valuable common ancestor of the plant tribe Fabeae and also important in breeding and agronomy studies of the cultivated Fabeae, but it is close to extinction. A concerted academic and geovernmental effort is needed to save it. Since 2007, an informal international group of researchers on legumes has been working to increase awareness of Vavilovia formosa (Stev.) Fed., a relict and endangered wild-land relative to crop plant species. A majority of the modern botanical classifications place it within the tribe Fabeae, together with the genera vetchling (Lathyrus L.), lentil (Lens Mill.), pea (Pisum L.) and vetch (Vicia L.). V. formosa is encountered at altitudes from 1,500 m up to 3,500 m in Armenia, Azerbaijan, Georgia, Iran, Iraq, Lebanon, Russia, Syria and Turkey. This species may be of extraordinary importance for broadening current scientific knowledge on legume evolution and taxonomy because of its proximity to the hypothetical common ancestor of the tribe Fabeae, as well as for breeding and agronomy of the cultivated Fabeae species due to its perenniality and stress resistance. All this may be feasible only if a concerted and long-term conservation strategy is established and carried out by both academic and geovernmental authorities. The existing populations of V. formosa are in serious danger of extinction. The main threats are domestic and wild animal grazing, foraging, and early frosts in late summer. A long-term strategy to save V. formosa from extinction and to sustain its use in both basic and applied research comprises much improved in situ preservation, greater efforts for an ex situ conservation, and novel approaches of in vitro propagation.
PB  - Springer, New York
T2  - Planta
T1  - Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed.
EP  - 1146
IS  - 5
SP  - 1139
VL  - 240
DO  - 10.1007/s00425-014-2136-9
ER  - 

@article{
author = "Mikić, Aleksandar and Smykal, Petr and Kenicer, Gregory and Vishnyakova, Margarita and Sarukhanyan, Nune and Akopian, Janna and Vanyan, Armen and Gabrielyan, Ivan and Smykalova, Iva and Sherbakova, Ekaterina and Zorić, Lana and Atlagić, Jovanka and Zeremski-Škorić, Tijana and Ćupina, Branko and Krstić, Đorđe and Jajić, Igor and Antanasović, Svetlana and Đorđević, Vuk and Mihailović, Vojislav and Ivanov, Alexandr and Ochatt, Sergio and Toker, Cengiz and Zlatković, Bojan and Ambrose, Mike",
year = "2014",
abstract = "Main conclusion Vavilovia formosa (Stev.) Fed. is a scientifically valuable common ancestor of the plant tribe Fabeae and also important in breeding and agronomy studies of the cultivated Fabeae, but it is close to extinction. A concerted academic and geovernmental effort is needed to save it. Since 2007, an informal international group of researchers on legumes has been working to increase awareness of Vavilovia formosa (Stev.) Fed., a relict and endangered wild-land relative to crop plant species. A majority of the modern botanical classifications place it within the tribe Fabeae, together with the genera vetchling (Lathyrus L.), lentil (Lens Mill.), pea (Pisum L.) and vetch (Vicia L.). V. formosa is encountered at altitudes from 1,500 m up to 3,500 m in Armenia, Azerbaijan, Georgia, Iran, Iraq, Lebanon, Russia, Syria and Turkey. This species may be of extraordinary importance for broadening current scientific knowledge on legume evolution and taxonomy because of its proximity to the hypothetical common ancestor of the tribe Fabeae, as well as for breeding and agronomy of the cultivated Fabeae species due to its perenniality and stress resistance. All this may be feasible only if a concerted and long-term conservation strategy is established and carried out by both academic and geovernmental authorities. The existing populations of V. formosa are in serious danger of extinction. The main threats are domestic and wild animal grazing, foraging, and early frosts in late summer. A long-term strategy to save V. formosa from extinction and to sustain its use in both basic and applied research comprises much improved in situ preservation, greater efforts for an ex situ conservation, and novel approaches of in vitro propagation.",
publisher = "Springer, New York",
journal = "Planta",
title = "Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed.",
pages = "1146-1139",
number = "5",
volume = "240",
doi = "10.1007/s00425-014-2136-9"
}

Mikić, A., Smykal, P., Kenicer, G., Vishnyakova, M., Sarukhanyan, N., Akopian, J., Vanyan, A., Gabrielyan, I., Smykalova, I., Sherbakova, E., Zorić, L., Atlagić, J., Zeremski-Škorić, T., Ćupina, B., Krstić, Đ., Jajić, I., Antanasović, S., Đorđević, V., Mihailović, V., Ivanov, A., Ochatt, S., Toker, C., Zlatković, B.,& Ambrose, M.. (2014). Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed.. in Planta
Springer, New York., 240(5), 1139-1146.
https://doi.org/10.1007/s00425-014-2136-9

Mikić A, Smykal P, Kenicer G, Vishnyakova M, Sarukhanyan N, Akopian J, Vanyan A, Gabrielyan I, Smykalova I, Sherbakova E, Zorić L, Atlagić J, Zeremski-Škorić T, Ćupina B, Krstić Đ, Jajić I, Antanasović S, Đorđević V, Mihailović V, Ivanov A, Ochatt S, Toker C, Zlatković B, Ambrose M. Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed.. in Planta. 2014;240(5):1139-1146.
doi:10.1007/s00425-014-2136-9 .

Mikić, Aleksandar, Smykal, Petr, Kenicer, Gregory, Vishnyakova, Margarita, Sarukhanyan, Nune, Akopian, Janna, Vanyan, Armen, Gabrielyan, Ivan, Smykalova, Iva, Sherbakova, Ekaterina, Zorić, Lana, Atlagić, Jovanka, Zeremski-Škorić, Tijana, Ćupina, Branko, Krstić, Đorđe, Jajić, Igor, Antanasović, Svetlana, Đorđević, Vuk, Mihailović, Vojislav, Ivanov, Alexandr, Ochatt, Sergio, Toker, Cengiz, Zlatković, Bojan, Ambrose, Mike, "Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed." in Planta, 240, no. 5 (2014):1139-1146,
https://doi.org/10.1007/s00425-014-2136-9 . .

TY  - JOUR
AU  - Mikić, Aleksandar
AU  - Smykal, Petr
AU  - Kenicer, Gregory
AU  - Vishnyakova, Margarita
AU  - Sarukhanyan, Nune
AU  - Akopian, Janna
AU  - Vanyan, Armen
AU  - Gabrielyan, Ivan
AU  - Smykalova, Iva
AU  - Sherbakova, Ekaterina
AU  - Zorić, Lana
AU  - Atlagić, Jovanka
AU  - Zeremski-Škorić, Tijana
AU  - Ćupina, Branko
AU  - Krstić, Đorđe
AU  - Jajić, Igor
AU  - Antanasović, Svetlana
AU  - Đorđević, Vuk
AU  - Mihailović, Vojislav
AU  - Ivanov, Alexandr
AU  - Ochatt, Sergio
AU  - Toker, Cengiz
AU  - Zlatković, Bojan
AU  - Ambrose, Mike
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1317
PB  - Springer, New York
T2  - Planta
T1  - Erratum to: Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed. (vol 240, pg 1139, 2014)
EP  - 1147
IS  - 5
SP  - 1147
VL  - 240
DO  - 10.1007/s00425-014-2173-4
ER  - 

@article{
author = "Mikić, Aleksandar and Smykal, Petr and Kenicer, Gregory and Vishnyakova, Margarita and Sarukhanyan, Nune and Akopian, Janna and Vanyan, Armen and Gabrielyan, Ivan and Smykalova, Iva and Sherbakova, Ekaterina and Zorić, Lana and Atlagić, Jovanka and Zeremski-Škorić, Tijana and Ćupina, Branko and Krstić, Đorđe and Jajić, Igor and Antanasović, Svetlana and Đorđević, Vuk and Mihailović, Vojislav and Ivanov, Alexandr and Ochatt, Sergio and Toker, Cengiz and Zlatković, Bojan and Ambrose, Mike",
year = "2014",
publisher = "Springer, New York",
journal = "Planta",
title = "Erratum to: Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed. (vol 240, pg 1139, 2014)",
pages = "1147-1147",
number = "5",
volume = "240",
doi = "10.1007/s00425-014-2173-4"
}

Mikić, A., Smykal, P., Kenicer, G., Vishnyakova, M., Sarukhanyan, N., Akopian, J., Vanyan, A., Gabrielyan, I., Smykalova, I., Sherbakova, E., Zorić, L., Atlagić, J., Zeremski-Škorić, T., Ćupina, B., Krstić, Đ., Jajić, I., Antanasović, S., Đorđević, V., Mihailović, V., Ivanov, A., Ochatt, S., Toker, C., Zlatković, B.,& Ambrose, M.. (2014). Erratum to: Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed. (vol 240, pg 1139, 2014). in Planta
Springer, New York., 240(5), 1147-1147.
https://doi.org/10.1007/s00425-014-2173-4

Mikić A, Smykal P, Kenicer G, Vishnyakova M, Sarukhanyan N, Akopian J, Vanyan A, Gabrielyan I, Smykalova I, Sherbakova E, Zorić L, Atlagić J, Zeremski-Škorić T, Ćupina B, Krstić Đ, Jajić I, Antanasović S, Đorđević V, Mihailović V, Ivanov A, Ochatt S, Toker C, Zlatković B, Ambrose M. Erratum to: Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed. (vol 240, pg 1139, 2014). in Planta. 2014;240(5):1147-1147.
doi:10.1007/s00425-014-2173-4 .

Mikić, Aleksandar, Smykal, Petr, Kenicer, Gregory, Vishnyakova, Margarita, Sarukhanyan, Nune, Akopian, Janna, Vanyan, Armen, Gabrielyan, Ivan, Smykalova, Iva, Sherbakova, Ekaterina, Zorić, Lana, Atlagić, Jovanka, Zeremski-Škorić, Tijana, Ćupina, Branko, Krstić, Đorđe, Jajić, Igor, Antanasović, Svetlana, Đorđević, Vuk, Mihailović, Vojislav, Ivanov, Alexandr, Ochatt, Sergio, Toker, Cengiz, Zlatković, Bojan, Ambrose, Mike, "Erratum to: Beauty will save the world, but will the world save beauty? The case of the highly endangered Vavilovia formosa (Stev.) Fed. (vol 240, pg 1139, 2014)" in Planta, 240, no. 5 (2014):1147-1147,
https://doi.org/10.1007/s00425-014-2173-4 . .

TY  - JOUR
AU  - Smykal, Petr
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Zlatković, Bojan
AU  - Ćupina, Branko
AU  - Đorđević, Vuk
AU  - Mikić, Aleksandar
AU  - Medović, Aleksandar
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1380
AB  - The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.
PB  - Springer, Dordrecht
T2  - Genetic Resources & Crop Evolution
T1  - A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication
EP  - 1544
IS  - 8
SP  - 1533
VL  - 61
DO  - 10.1007/s10722-014-0128-z
ER  - 

@article{
author = "Smykal, Petr and Jovanović, Živko and Stanisavljević, Nemanja and Zlatković, Bojan and Ćupina, Branko and Đorđević, Vuk and Mikić, Aleksandar and Medović, Aleksandar",
year = "2014",
abstract = "The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources & Crop Evolution",
title = "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication",
pages = "1544-1533",
number = "8",
volume = "61",
doi = "10.1007/s10722-014-0128-z"
}

Smykal, P., Jovanović, Ž., Stanisavljević, N., Zlatković, B., Ćupina, B., Đorđević, V., Mikić, A.,& Medović, A.. (2014). A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources & Crop Evolution
Springer, Dordrecht., 61(8), 1533-1544.
https://doi.org/10.1007/s10722-014-0128-z

Smykal P, Jovanović Ž, Stanisavljević N, Zlatković B, Ćupina B, Đorđević V, Mikić A, Medović A. A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources & Crop Evolution. 2014;61(8):1533-1544.
doi:10.1007/s10722-014-0128-z .

Smykal, Petr, Jovanović, Živko, Stanisavljević, Nemanja, Zlatković, Bojan, Ćupina, Branko, Đorđević, Vuk, Mikić, Aleksandar, Medović, Aleksandar, "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication" in Genetic Resources & Crop Evolution, 61, no. 8 (2014):1533-1544,
https://doi.org/10.1007/s10722-014-0128-z . .

TY  - CONF
AU  - Aleksić, Jelena
AU  - Banović, Bojana
AU  - Mikić, Aleksandar
AU  - Ćupina, Branko
AU  - Zlatković, Bojan
AU  - Anđelković, Snežana
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2862
AB  - An increasing interest in cultivation and improvement of faba bean (Vicia faba L.), an important food and feed grain legume whose ex siz genetic resources comprise cultivated forms only, triggers both search for novel genotypes and their molecular characterization. Due to the poor representation of faba bean genotypes from the central Balkans in worldwide ex situ collections, we collected seeds of 13 faba bean landraces from this region, preserved them at the French National Institute for Agricultural Research (INRA) ex situ collection and used them also for amplification of ten nuclear microsatellites (simple sequence repeats — SSRs). Given the self-pollinating nature of faba bean and low heterozygosity levels in this crop, we assumed that relatively low number of alleles may be expected in each landrace. Therefore, we first demonstrated that all alleles obtained upon amplification of ten SSRs in 11 individuals from one landrace could be detected when DNAs from these individuals were pooled and such a bulk DNA was used as a template for PCR reactions, and then tested all SSRs in bulk DNA templates from each out of ten remaining landraces comprising six to 15 genotypes.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.)
EP  - 19
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2862
ER  - 

@conference{
author = "Aleksić, Jelena and Banović, Bojana and Mikić, Aleksandar and Ćupina, Branko and Zlatković, Bojan and Anđelković, Snežana and Maksimović, Vesna",
year = "2013",
abstract = "An increasing interest in cultivation and improvement of faba bean (Vicia faba L.), an important food and feed grain legume whose ex siz genetic resources comprise cultivated forms only, triggers both search for novel genotypes and their molecular characterization. Due to the poor representation of faba bean genotypes from the central Balkans in worldwide ex situ collections, we collected seeds of 13 faba bean landraces from this region, preserved them at the French National Institute for Agricultural Research (INRA) ex situ collection and used them also for amplification of ten nuclear microsatellites (simple sequence repeats — SSRs). Given the self-pollinating nature of faba bean and low heterozygosity levels in this crop, we assumed that relatively low number of alleles may be expected in each landrace. Therefore, we first demonstrated that all alleles obtained upon amplification of ten SSRs in 11 individuals from one landrace could be detected when DNAs from these individuals were pooled and such a bulk DNA was used as a template for PCR reactions, and then tested all SSRs in bulk DNA templates from each out of ten remaining landraces comprising six to 15 genotypes.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.)",
pages = "19-19",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2862"
}

Aleksić, J., Banović, B., Mikić, A., Ćupina, B., Zlatković, B., Anđelković, S.,& Maksimović, V.. (2013). Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 19-19.
https://hdl.handle.net/21.15107/rcub_fiver_2862

Aleksić J, Banović B, Mikić A, Ćupina B, Zlatković B, Anđelković S, Maksimović V. Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:19-19.
https://hdl.handle.net/21.15107/rcub_fiver_2862 .

Aleksić, Jelena, Banović, Bojana, Mikić, Aleksandar, Ćupina, Branko, Zlatković, Bojan, Anđelković, Snežana, Maksimović, Vesna, "Preservation and molecular characterization of 13 central Balkan landraces of faba bean (Vicia faba L.)" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):19-19,
https://hdl.handle.net/21.15107/rcub_fiver_2862 .

TY  - CONF
AU  - Zlatković, Bojan
AU  - Mikić, Aleksandar
AU  - Đokić, Mrđan
AU  - Đorđević, Vuk
AU  - Ćupina, Branko
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2992
AB  - The flora of Pčinja valley, as situated in southern Balkan Peninsula, is comparatively rich in native legume species, representing important genomic and breeding resources for legume crops and forages. According to their taxonomical diversity, legume family belongs to the group of most important plant families in the flora of Serbia, and at the same time the second best represented in the flora of Pčinja valley. Considering elements different in origin and ecological requirements this family comprises impressive list of 100 species and subspecies that represents more than 40% of their total number in Serbia. At the same time such a large number of representatives are distributed across the area of hardly 0.17% of whole country area. The genus Trifolium, referring 32 taxa, is the most abundant among all other genera in the flora of the valley. The following genera: Vicia (15), Lathyrus (11), Medicago (7), Chamacytisus (5) and Astragalus (4) are also considerably rich at the family level. The life forms spectrum points at ecological group of annual representatives taking the largest portion of the all legume flora of valley.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Native legumes in the flora of Pčinja valley (South Serbia) — species diversity and distribution pattern
EP  - 45
SP  - 45
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2992
ER  - 

@conference{
author = "Zlatković, Bojan and Mikić, Aleksandar and Đokić, Mrđan and Đorđević, Vuk and Ćupina, Branko",
year = "2013",
abstract = "The flora of Pčinja valley, as situated in southern Balkan Peninsula, is comparatively rich in native legume species, representing important genomic and breeding resources for legume crops and forages. According to their taxonomical diversity, legume family belongs to the group of most important plant families in the flora of Serbia, and at the same time the second best represented in the flora of Pčinja valley. Considering elements different in origin and ecological requirements this family comprises impressive list of 100 species and subspecies that represents more than 40% of their total number in Serbia. At the same time such a large number of representatives are distributed across the area of hardly 0.17% of whole country area. The genus Trifolium, referring 32 taxa, is the most abundant among all other genera in the flora of the valley. The following genera: Vicia (15), Lathyrus (11), Medicago (7), Chamacytisus (5) and Astragalus (4) are also considerably rich at the family level. The life forms spectrum points at ecological group of annual representatives taking the largest portion of the all legume flora of valley.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Native legumes in the flora of Pčinja valley (South Serbia) — species diversity and distribution pattern",
pages = "45-45",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2992"
}

Zlatković, B., Mikić, A., Đokić, M., Đorđević, V.,& Ćupina, B.. (2013). Native legumes in the flora of Pčinja valley (South Serbia) — species diversity and distribution pattern. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 45-45.
https://hdl.handle.net/21.15107/rcub_fiver_2992

Zlatković B, Mikić A, Đokić M, Đorđević V, Ćupina B. Native legumes in the flora of Pčinja valley (South Serbia) — species diversity and distribution pattern. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:45-45.
https://hdl.handle.net/21.15107/rcub_fiver_2992 .

Zlatković, Bojan, Mikić, Aleksandar, Đokić, Mrđan, Đorđević, Vuk, Ćupina, Branko, "Native legumes in the flora of Pčinja valley (South Serbia) — species diversity and distribution pattern" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):45-45,
https://hdl.handle.net/21.15107/rcub_fiver_2992 .

TY  - CONF
AU  - Medović, Aleksandar
AU  - Smykal, Petr
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Zlatković, Bojan
AU  - Ćupina, Branko
AU  - Đorđević, Vuk
AU  - Mikić, Aleksandar
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2983
AB  - A unique example of 2,572 carbonized pea seeds was recovered from the 11th century B.C. deposits at Hissar, a multilevel settlement of the Brnjica cultural group (1,350-1,000 B.C.) in southeast Serbia. Five hundred seeds maintained pea-like hilum, only few had preserved intact smooth-surfaced testa and the majority of seeds had no seed coat. Fortunately, only few seeds of other pulses were observed in the sample and their identity was clearly determined. Applying the exclusion principle, all the “naked”, 3-4 mm large seeds, mainly broad ellipsoid and less often globose, with flattenings or concavities, were determined to correspond to cultivated pea (Pisum sativum L.). Additionally, a high thousand seed weight of charred seeds (24.4 g) suggested cultivated status. To confirm our finding, we processed two samples with molecular tools. A sequence analysis of four chloroplast DNA loci (trnSG, trnK, matK and rbcL) in total length of 1329bp, showed intermediate position to cultivated P. sativum and wild P. sativum subsp. elatius.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia
EP  - 28
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2983
ER  - 

@conference{
author = "Medović, Aleksandar and Smykal, Petr and Jovanović, Živko and Stanisavljević, Nemanja and Zlatković, Bojan and Ćupina, Branko and Đorđević, Vuk and Mikić, Aleksandar",
year = "2013",
abstract = "A unique example of 2,572 carbonized pea seeds was recovered from the 11th century B.C. deposits at Hissar, a multilevel settlement of the Brnjica cultural group (1,350-1,000 B.C.) in southeast Serbia. Five hundred seeds maintained pea-like hilum, only few had preserved intact smooth-surfaced testa and the majority of seeds had no seed coat. Fortunately, only few seeds of other pulses were observed in the sample and their identity was clearly determined. Applying the exclusion principle, all the “naked”, 3-4 mm large seeds, mainly broad ellipsoid and less often globose, with flattenings or concavities, were determined to correspond to cultivated pea (Pisum sativum L.). Additionally, a high thousand seed weight of charred seeds (24.4 g) suggested cultivated status. To confirm our finding, we processed two samples with molecular tools. A sequence analysis of four chloroplast DNA loci (trnSG, trnK, matK and rbcL) in total length of 1329bp, showed intermediate position to cultivated P. sativum and wild P. sativum subsp. elatius.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia",
pages = "28-28",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2983"
}

Medović, A., Smykal, P., Jovanović, Ž., Stanisavljević, N., Zlatković, B., Ćupina, B., Đorđević, V.,& Mikić, A.. (2013). A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 28-28.
https://hdl.handle.net/21.15107/rcub_fiver_2983

Medović A, Smykal P, Jovanović Ž, Stanisavljević N, Zlatković B, Ćupina B, Đorđević V, Mikić A. A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:28-28.
https://hdl.handle.net/21.15107/rcub_fiver_2983 .

Medović, Aleksandar, Smykal, Petr, Jovanović, Živko, Stanisavljević, Nemanja, Zlatković, Bojan, Ćupina, Branko, Đorđević, Vuk, Mikić, Aleksandar, "A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):28-28,
https://hdl.handle.net/21.15107/rcub_fiver_2983 .

TY  - CONF
AU  - Ćupina, Branko
AU  - Zlatković, Bojan
AU  - Antanasović, Svetlana
AU  - Đorđević, Vuk
AU  - Mikić, Aleksandar
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2982
AB  - European Legumes and Their Traditional Food and Feed Products SEELEGUMES) has been carried out within the EU SEE-ERA.NET Plus programme, gathering together 19 partnering organisations from 14 European countries. One of the greatest achievements of the project was a re-discovery of numerous crop wild relatives of cultivated annual legumes in local floras, with a specific emphasis on "tall" pea (Pisum sativum L. subsp. elatius Steven ex M. Bieb.) Asch. & Graebn.). During 2011 and 2012, five expeditions have been made to the upper flow of the river Pčinja and on the slopes of the mountain Kozjak, where a population of Pisum sativum L. subsp. elatius was monitored in situ, from the stages of full bloom in late May 2011, full seed maturity in late June 
2011, autumn emergence in October 2011, spring revival in March 2012 and the next reproductive stage in early June 2012. A morphological and chemical analysis of the forage and grain dry matter confirmed a considerable potential of Pisum sativum L. subsp. elatius for cultivation, while a prominent resistance to low temperatures has also been assessed.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - A contribution of the project SEELEGUMES to the re-discovery of Pisum elatius subsp. elatius in Serbia and FYR of Macedonia
EP  - 23
SP  - 23
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2982
ER  - 

@conference{
author = "Ćupina, Branko and Zlatković, Bojan and Antanasović, Svetlana and Đorđević, Vuk and Mikić, Aleksandar",
year = "2013",
abstract = "European Legumes and Their Traditional Food and Feed Products SEELEGUMES) has been carried out within the EU SEE-ERA.NET Plus programme, gathering together 19 partnering organisations from 14 European countries. One of the greatest achievements of the project was a re-discovery of numerous crop wild relatives of cultivated annual legumes in local floras, with a specific emphasis on "tall" pea (Pisum sativum L. subsp. elatius Steven ex M. Bieb.) Asch. & Graebn.). During 2011 and 2012, five expeditions have been made to the upper flow of the river Pčinja and on the slopes of the mountain Kozjak, where a population of Pisum sativum L. subsp. elatius was monitored in situ, from the stages of full bloom in late May 2011, full seed maturity in late June 
2011, autumn emergence in October 2011, spring revival in March 2012 and the next reproductive stage in early June 2012. A morphological and chemical analysis of the forage and grain dry matter confirmed a considerable potential of Pisum sativum L. subsp. elatius for cultivation, while a prominent resistance to low temperatures has also been assessed.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "A contribution of the project SEELEGUMES to the re-discovery of Pisum elatius subsp. elatius in Serbia and FYR of Macedonia",
pages = "23-23",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2982"
}

Ćupina, B., Zlatković, B., Antanasović, S., Đorđević, V.,& Mikić, A.. (2013). A contribution of the project SEELEGUMES to the re-discovery of Pisum elatius subsp. elatius in Serbia and FYR of Macedonia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 23-23.
https://hdl.handle.net/21.15107/rcub_fiver_2982

Ćupina B, Zlatković B, Antanasović S, Đorđević V, Mikić A. A contribution of the project SEELEGUMES to the re-discovery of Pisum elatius subsp. elatius in Serbia and FYR of Macedonia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:23-23.
https://hdl.handle.net/21.15107/rcub_fiver_2982 .

Ćupina, Branko, Zlatković, Bojan, Antanasović, Svetlana, Đorđević, Vuk, Mikić, Aleksandar, "A contribution of the project SEELEGUMES to the re-discovery of Pisum elatius subsp. elatius in Serbia and FYR of Macedonia" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):23-23,
https://hdl.handle.net/21.15107/rcub_fiver_2982 .

TY  - JOUR
AU  - Mikić, Aleksandar
AU  - Mihailović, Vojislav
AU  - Ćupina, Branko
AU  - Antanasović, Svetlana
AU  - Krstić, Đorđe
AU  - Zlatković, Bojan
AU  - Đorđević, Vuk
AU  - Zorić, Lana
AU  - Taški-Ajduković, Ksenija
AU  - Nagl, Nevena
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1269
AB  - Large-flowered vetch (Vicia grandiflora Scop.) is widely present in many Eurasian wild floras, where it has significance in improving grassland communities and environment-friendly uses as a cover crop and green manure. The main goal of this study was to assess the possibility of transforming large-flowered vetch into a field crop and assess the basics for its breeding. A small-plot trial was carried out in three consecutive growing seasons 2005-2008 in Novi Sad, including twelve large-flowered wild populations collected at various sites in Serbia and France from 2002 to 2004 and multiplied ex situ during subsequent years. The population MM 02/01 of Serbian origin had the highest three-year average proportion of plants surviving winter (0.96). The smallest average number of days from sowing to first flower was in the population MM 03/10 (199 days). The population MM 03/08 produced the highest forage dry matter (8.0 Mg ha(-1)) yield stage between full flowering and forming first pods. The highest seed yield was in the population MM 03/10 (2,084 kg ha(-1)). Forage dry matter yield was significantly correlated to main stem length (r = 0.932), number of fertile nodes (r = 0.710), number of internodes (r = 0.640) and number of leaves (r = 0.604). Seed yield was significantly correlated to number of pods (r = 0.930), number of seeds (r = 0.883), number of stems (r = 0.882) and number of fertile nodes (r = 0.768). Our study confirmed that large-flowered vetch may represent a stable and quality source of forage in temperate regions such as the Balkans, as well as that it may have its place in various crop rotations and farming systems. At the same time, the existing phenotypic variability offers a solid basis for further improvement of this species.
PB  - Springer, Dordrecht
T2  - Euphytica
T1  - Ex situ evaluation of cultivation potential in wild populations of large-flowered vetch (Vicia grandiflora)
EP  - 12
IS  - 1
SP  - 1
VL  - 193
DO  - 10.1007/s10681-013-0872-8
ER  - 

@article{
author = "Mikić, Aleksandar and Mihailović, Vojislav and Ćupina, Branko and Antanasović, Svetlana and Krstić, Đorđe and Zlatković, Bojan and Đorđević, Vuk and Zorić, Lana and Taški-Ajduković, Ksenija and Nagl, Nevena",
year = "2013",
abstract = "Large-flowered vetch (Vicia grandiflora Scop.) is widely present in many Eurasian wild floras, where it has significance in improving grassland communities and environment-friendly uses as a cover crop and green manure. The main goal of this study was to assess the possibility of transforming large-flowered vetch into a field crop and assess the basics for its breeding. A small-plot trial was carried out in three consecutive growing seasons 2005-2008 in Novi Sad, including twelve large-flowered wild populations collected at various sites in Serbia and France from 2002 to 2004 and multiplied ex situ during subsequent years. The population MM 02/01 of Serbian origin had the highest three-year average proportion of plants surviving winter (0.96). The smallest average number of days from sowing to first flower was in the population MM 03/10 (199 days). The population MM 03/08 produced the highest forage dry matter (8.0 Mg ha(-1)) yield stage between full flowering and forming first pods. The highest seed yield was in the population MM 03/10 (2,084 kg ha(-1)). Forage dry matter yield was significantly correlated to main stem length (r = 0.932), number of fertile nodes (r = 0.710), number of internodes (r = 0.640) and number of leaves (r = 0.604). Seed yield was significantly correlated to number of pods (r = 0.930), number of seeds (r = 0.883), number of stems (r = 0.882) and number of fertile nodes (r = 0.768). Our study confirmed that large-flowered vetch may represent a stable and quality source of forage in temperate regions such as the Balkans, as well as that it may have its place in various crop rotations and farming systems. At the same time, the existing phenotypic variability offers a solid basis for further improvement of this species.",
publisher = "Springer, Dordrecht",
journal = "Euphytica",
title = "Ex situ evaluation of cultivation potential in wild populations of large-flowered vetch (Vicia grandiflora)",
pages = "12-1",
number = "1",
volume = "193",
doi = "10.1007/s10681-013-0872-8"
}

Mikić, A., Mihailović, V., Ćupina, B., Antanasović, S., Krstić, Đ., Zlatković, B., Đorđević, V., Zorić, L., Taški-Ajduković, K.,& Nagl, N.. (2013). Ex situ evaluation of cultivation potential in wild populations of large-flowered vetch (Vicia grandiflora). in Euphytica
Springer, Dordrecht., 193(1), 1-12.
https://doi.org/10.1007/s10681-013-0872-8

Mikić A, Mihailović V, Ćupina B, Antanasović S, Krstić Đ, Zlatković B, Đorđević V, Zorić L, Taški-Ajduković K, Nagl N. Ex situ evaluation of cultivation potential in wild populations of large-flowered vetch (Vicia grandiflora). in Euphytica. 2013;193(1):1-12.
doi:10.1007/s10681-013-0872-8 .

Mikić, Aleksandar, Mihailović, Vojislav, Ćupina, Branko, Antanasović, Svetlana, Krstić, Đorđe, Zlatković, Bojan, Đorđević, Vuk, Zorić, Lana, Taški-Ajduković, Ksenija, Nagl, Nevena, "Ex situ evaluation of cultivation potential in wild populations of large-flowered vetch (Vicia grandiflora)" in Euphytica, 193, no. 1 (2013):1-12,
https://doi.org/10.1007/s10681-013-0872-8 . .