TY  - CONF
AU  - Dorić, Dušica
AU  - Malenčić, Djordje
AU  - Kiprovski, Biljana
AU  - Barać, Goran
AU  - Ljubojević, Mirjana
AU  - Ognjanov, Vladislav
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/2326
AB  - Oxidative metabolism of normal cells and different stress situations generate highly reactive oxygen
species (ROS). The ROS have been implicated in a number of physiological disorders in plants. Such factors as certain mechanical injuries are known to induce ROS formation in most aerobic organisms. Antioxidant defence systems have co-evolved with aerobic metabolism to counteract oxidative damage from ROS. This includes antioxidant enzymes such as superoxide-dismutase (SOD; EC 1.15.1.1). SOD is of the greatest importance since it catalyzes the dismutation of the superoxide radical (O2.-) to O2 and H2O. Thus, the aim of this study was to measure the intensity of SOD in the leaves of six cherry rootstock selections in order to investigate the effect of exogenously applied auxins on the mechanical injury induced oxidative stress during the process of rooting of softwood cuttings.
The experiment was carried out in the plastic house under a fogging system with 95-99% average relative
humidity. The 15-20 cm long terminal cuttings were treated with the mix of auxins (IBA, 0.5% and α-NAA, 0.8%)
powder prior to insertion into the white sphagnum and perlite substrate mixture. Leaves of six promising rootstock selections of Prunus cerasus L., P. mahaleb L. and P. fruticosa Pall., and one standard rootstock PHL-A were sampled on the 1st, 3rd and 7th day after inserting softwood cuttings into the rooting substrate. The SOD activity was measured by monitoring the inhibition of nitroblue-tetrazolium (NBT) reduction at 560 nm.
Results showed that generation of ROS occurred in all investigated genotypes, but they greatly differed in
susceptibility towards oxidative stress induces by mechanical injury. Application of auxins on standard vegetative rootstock PHL-A, as well as P. mahaleb selection M6 showed positive effect on lowering SOD activity after the 3rd and 7th collecting day compared to control. On the other hand, selections of P. cerasus OV22 and P. fruticosa SV2 expressed high SOD activity during all collecting days which was from 3.6-54.9% higher compared to control.
Enhanced SOD activity in leaves of softwood cuttings indicates induction of the enzymatic antioxidant system in
both auxin treated and untreated cutting. According to these results SOD activity could be used as one of the
biochemical parameters in further rootstock selection for sweet and sour cherries.
PB  - International Organization for Biological and Integrated Control (IOBC)
C3  - Book of Abstracts, 9th International Conference on Integrated Fruit Production, 04-08.09.2016, Thessaloniki, Greece
T1  - Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress
EP  - 167
SP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2326
ER  - 

@conference{
author = "Dorić, Dušica and Malenčić, Djordje and Kiprovski, Biljana and Barać, Goran and Ljubojević, Mirjana and Ognjanov, Vladislav",
year = "2016",
abstract = "Oxidative metabolism of normal cells and different stress situations generate highly reactive oxygen
species (ROS). The ROS have been implicated in a number of physiological disorders in plants. Such factors as certain mechanical injuries are known to induce ROS formation in most aerobic organisms. Antioxidant defence systems have co-evolved with aerobic metabolism to counteract oxidative damage from ROS. This includes antioxidant enzymes such as superoxide-dismutase (SOD; EC 1.15.1.1). SOD is of the greatest importance since it catalyzes the dismutation of the superoxide radical (O2.-) to O2 and H2O. Thus, the aim of this study was to measure the intensity of SOD in the leaves of six cherry rootstock selections in order to investigate the effect of exogenously applied auxins on the mechanical injury induced oxidative stress during the process of rooting of softwood cuttings.
The experiment was carried out in the plastic house under a fogging system with 95-99% average relative
humidity. The 15-20 cm long terminal cuttings were treated with the mix of auxins (IBA, 0.5% and α-NAA, 0.8%)
powder prior to insertion into the white sphagnum and perlite substrate mixture. Leaves of six promising rootstock selections of Prunus cerasus L., P. mahaleb L. and P. fruticosa Pall., and one standard rootstock PHL-A were sampled on the 1st, 3rd and 7th day after inserting softwood cuttings into the rooting substrate. The SOD activity was measured by monitoring the inhibition of nitroblue-tetrazolium (NBT) reduction at 560 nm.
Results showed that generation of ROS occurred in all investigated genotypes, but they greatly differed in
susceptibility towards oxidative stress induces by mechanical injury. Application of auxins on standard vegetative rootstock PHL-A, as well as P. mahaleb selection M6 showed positive effect on lowering SOD activity after the 3rd and 7th collecting day compared to control. On the other hand, selections of P. cerasus OV22 and P. fruticosa SV2 expressed high SOD activity during all collecting days which was from 3.6-54.9% higher compared to control.
Enhanced SOD activity in leaves of softwood cuttings indicates induction of the enzymatic antioxidant system in
both auxin treated and untreated cutting. According to these results SOD activity could be used as one of the
biochemical parameters in further rootstock selection for sweet and sour cherries.",
publisher = "International Organization for Biological and Integrated Control (IOBC)",
journal = "Book of Abstracts, 9th International Conference on Integrated Fruit Production, 04-08.09.2016, Thessaloniki, Greece",
title = "Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress",
pages = "167-167",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2326"
}

Dorić, D., Malenčić, D., Kiprovski, B., Barać, G., Ljubojević, M.,& Ognjanov, V.. (2016). Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress. in Book of Abstracts, 9th International Conference on Integrated Fruit Production, 04-08.09.2016, Thessaloniki, Greece
International Organization for Biological and Integrated Control (IOBC)., 167-167.
https://hdl.handle.net/21.15107/rcub_fiver_2326

Dorić D, Malenčić D, Kiprovski B, Barać G, Ljubojević M, Ognjanov V. Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress. in Book of Abstracts, 9th International Conference on Integrated Fruit Production, 04-08.09.2016, Thessaloniki, Greece. 2016;:167-167.
https://hdl.handle.net/21.15107/rcub_fiver_2326 .

Dorić, Dušica, Malenčić, Djordje, Kiprovski, Biljana, Barać, Goran, Ljubojević, Mirjana, Ognjanov, Vladislav, "Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress" in Book of Abstracts, 9th International Conference on Integrated Fruit Production, 04-08.09.2016, Thessaloniki, Greece (2016):167-167,
https://hdl.handle.net/21.15107/rcub_fiver_2326 .

TY  - JOUR
AU  - Dorić, Dušica
AU  - Kiprovski, Biljana
AU  - Malenčić, Đorđe
AU  - Ognjanov, Vladislav
AU  - Ljubojević, Mirjana
AU  - Barać, Goran
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/2210
AB  - The effect of exogenously applied auxins on mechanical injury induced oxidative stress was studied in 6 promising rootstock selections of Prunus cerasus L., P. mahaleb L., and P. fruticosa Pall. Investigated selections were included in low vigorous rootstock breeding programme for sweet and sour cherries. The standard rootstock PHL-A was used as a control, due to successful rooting. Leaves of investigated rootstocks were collected on: 0, 1st, 3rd and 7th day of inserting softwood cuttings into the rooting substrate under a fogging system with 95-99% average relative humidity. Exogenously applied auxin mixture consisted of 0.8% α-naphthylacetic acid (NAA) and 0.5% indolebutyric acid (IBA). Mechanical injuries during rooting period represent one of the factors that induce stress in softwood cuttings. Intensity of LP is used as a valuable biomarker of plant response to various abiotic factors. Differences in intensity of LP between auxin-treated and -untreated cuttings were examined. Almost all of investigated selections had lower LP intensity after auxin application (11.4-47.2%) between 1st and 3rd day. However, the most prominent change was in leaves of PHL-A (49.2%). The best LP-lowering effect were recorded in leaves of P. fruticosa, SV4 selection (56.9%) on 7th day and in P. mahaleb, M4 selection, on 1st and 3rd day (5.9, 5.8%), in comparison to untreated softwood cuttings. As for OV21 selection, LP intensity significantly increased in both treated and untreated cuttings on the 1st, 3rd and 7th day, but auxin-treated cuttings showed lower LP values, except on 7th day, reaching 221.3 nmol MDA equivalents g-1 fresh weight. Intensity of LP during vegetative propagation of selected genotypes could be used as one of the biochemical parameters in further rootstock selection for sweet and sour cherries.
PB  - Novi Sad : University of Novi Sad, Faculty of Agriculture
T2  - Contemporary Agriculture
T1  - Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins
T1  - Smanjenje intenziteta lipidne peroksidacije u listovima selekcija vegetativnih podloga za višnju i trešnju upotrebom egzogeno primenjenih auksina
EP  - 85
IS  - 1-2
SP  - 78
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2210
ER  - 

@article{
author = "Dorić, Dušica and Kiprovski, Biljana and Malenčić, Đorđe and Ognjanov, Vladislav and Ljubojević, Mirjana and Barać, Goran",
year = "2015",
abstract = "The effect of exogenously applied auxins on mechanical injury induced oxidative stress was studied in 6 promising rootstock selections of Prunus cerasus L., P. mahaleb L., and P. fruticosa Pall. Investigated selections were included in low vigorous rootstock breeding programme for sweet and sour cherries. The standard rootstock PHL-A was used as a control, due to successful rooting. Leaves of investigated rootstocks were collected on: 0, 1st, 3rd and 7th day of inserting softwood cuttings into the rooting substrate under a fogging system with 95-99% average relative humidity. Exogenously applied auxin mixture consisted of 0.8% α-naphthylacetic acid (NAA) and 0.5% indolebutyric acid (IBA). Mechanical injuries during rooting period represent one of the factors that induce stress in softwood cuttings. Intensity of LP is used as a valuable biomarker of plant response to various abiotic factors. Differences in intensity of LP between auxin-treated and -untreated cuttings were examined. Almost all of investigated selections had lower LP intensity after auxin application (11.4-47.2%) between 1st and 3rd day. However, the most prominent change was in leaves of PHL-A (49.2%). The best LP-lowering effect were recorded in leaves of P. fruticosa, SV4 selection (56.9%) on 7th day and in P. mahaleb, M4 selection, on 1st and 3rd day (5.9, 5.8%), in comparison to untreated softwood cuttings. As for OV21 selection, LP intensity significantly increased in both treated and untreated cuttings on the 1st, 3rd and 7th day, but auxin-treated cuttings showed lower LP values, except on 7th day, reaching 221.3 nmol MDA equivalents g-1 fresh weight. Intensity of LP during vegetative propagation of selected genotypes could be used as one of the biochemical parameters in further rootstock selection for sweet and sour cherries.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Agriculture",
journal = "Contemporary Agriculture",
title = "Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins, Smanjenje intenziteta lipidne peroksidacije u listovima selekcija vegetativnih podloga za višnju i trešnju upotrebom egzogeno primenjenih auksina",
pages = "85-78",
number = "1-2",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2210"
}

Dorić, D., Kiprovski, B., Malenčić, Đ., Ognjanov, V., Ljubojević, M.,& Barać, G.. (2015). Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins. in Contemporary Agriculture
Novi Sad : University of Novi Sad, Faculty of Agriculture., 64(1-2), 78-85.
https://hdl.handle.net/21.15107/rcub_fiver_2210

Dorić D, Kiprovski B, Malenčić Đ, Ognjanov V, Ljubojević M, Barać G. Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins. in Contemporary Agriculture. 2015;64(1-2):78-85.
https://hdl.handle.net/21.15107/rcub_fiver_2210 .

Dorić, Dušica, Kiprovski, Biljana, Malenčić, Đorđe, Ognjanov, Vladislav, Ljubojević, Mirjana, Barać, Goran, "Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins" in Contemporary Agriculture, 64, no. 1-2 (2015):78-85,
https://hdl.handle.net/21.15107/rcub_fiver_2210 .