TY  - JOUR
AU  - Umićević, Sonja
AU  - Antić, Marina
AU  - Rajković, Dragana
AU  - Sinkovič, Lovro
AU  - Nagl, Nevena
AU  - Goreta Ban, Smiljana
AU  - Meglič, Vladimir
AU  - Todorović, Vida
AU  - Marjanović-Jeromela, Ana
AU  - Pipan, Barbara
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3784
AB  - Leafy kale (Brassica oleracea var. acephala L.) is one of the most
important Brassicaceae species with health-promoting properties and great
diversity. To investigate variability within and among accessions, 12 accessions
(each accession comprised four individuals/genotypes) from the Balkan region
were genotyped at 12 simple sequence repeats (SSRs) loci. The selected SSR
markers originated from the genomes of B. oleracea (eight), B. rapa (two) and
B. napus (two). DNA was extracted from seeds, cotyledons, shoots, and young
leaves, depending on the germination energy of each seed, using magnetic
extraction method. Genetic analysis included eight accessions from Serbia (24
genotypes), two accessions from Montenegro (eight genotypes), and one
accession from Bosnia and Herzegovina and Croatia (with four genotypes each).
Accession-specific polymorphism was found at the Ol12-FO2 and Ol10-F11a
loci, where genotypes were qualitatively distinguished by horizontal agarose gel
electrophoresis (binary detection). Among 12 loci, the average polymorphic
information content (PIC) was 0.71, with the marker Ol13-C12 proving to be the
78 Umićević et al.
most informative (PIC = 0.89). However, the analysis of molecular variability
(AMOVA) showed 11% diversity between accessions, 19% diversity between
genotypes, and 70% diversity within genotypes, explaining the molecular
diversity of native Balkan leafy kale germplasm. According to the genetic
structure, four genetic groups were formed with an average expected
heterozygosity of 0.70 between clusters. In order to exploit the genetic diversity,
it would be advisable to evaluate these accessions at phenotypic level and use
their potential in breeding programmes.
AB  - Raštan (Brassica oleracea var. acephala L.) je jedna od najvažnijih vrsta iz porodice kupusnjača koja povoljno utiče na zdravlje ljudi i odlikuje se velikom raznovrsnošću. Da bi se istražila varijabilnost unutar i između prinova, 12 prinova (svaka prinova se sastojala od četiri individue/genotipa) sa teritorije Balkanskog poluostrva je genotipizovano koristeći 12 SSR markera. Odabrani SSR markeri potiču iz genoma B. oleracea (osam), B. rapa (dva) i B. napus (dva). DNK je izolovana iz sjemena, kotiledona, izdanaka i mladih listova, u zavisnosti od energije klijanja svakog sjemena, metodom magnetne ekstrakcije. Genetičkom analizom obuhvaćeno je osam prinova iz Srbije (24 genotipa), dvije prinove iz Crne Gore (osam genotipova) i jedna prinova iz Bosne i Hercegovine i Hrvatske (sa po četiri genotipa). Polimorfizam specifičan za prinove pronađen je na lokusima Ol12-FO2 i Ol10-F11a, gdje su genotipovi kvalitativno razlikovani horizontalnom elektroforezom u agaroznom gelu (binarna detekcija). Kod ukupno 12 lokusa, prosječan sadržaj polimorfne informacije (PIC) bio je 0,71, pri čemu se kao najinformativniji pokazao marker Ol13-C12 (PIC = 0,89). Međutim, analiza molekularne varijabilnosti (AMOVA) pokazala je 11% raznovrsnosti između prinova, 19% raznovrsnosti između genotipova i 70% raznovrsnosti unutar genotipova, objašnjavajući molekularnu raznovrsnost izvorne germplazme raštana sa teritorije Balkanskog poluostrva. Prema genetičkoj strukturi formirane su četiri genetičke grupe sa prosječnom očekivanom heterozigotnošću od 0,70 između klastera. Da bi se iskoristio genetički diverzitet, bilo bi preporučljivo da se ove prinove karakterišu 90 Umićević et al. na fenotipskom nivou i da se njihov potencijal iskoristi u programima oplemenjivanja.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
T2  - Agroznanje / Agro-knowledge Journal
T1  - Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.)
T1  - Genetički diverzitet genetičkih resursa raštana (Brassica oleracea var. acephala L.)
EP  - 90
IS  - 3
SP  - 77
VL  - 24
DO  - 10.7251/AGREN2303077U
ER  - 

@article{
author = "Umićević, Sonja and Antić, Marina and Rajković, Dragana and Sinkovič, Lovro and Nagl, Nevena and Goreta Ban, Smiljana and Meglič, Vladimir and Todorović, Vida and Marjanović-Jeromela, Ana and Pipan, Barbara",
year = "2023",
abstract = "Leafy kale (Brassica oleracea var. acephala L.) is one of the most
important Brassicaceae species with health-promoting properties and great
diversity. To investigate variability within and among accessions, 12 accessions
(each accession comprised four individuals/genotypes) from the Balkan region
were genotyped at 12 simple sequence repeats (SSRs) loci. The selected SSR
markers originated from the genomes of B. oleracea (eight), B. rapa (two) and
B. napus (two). DNA was extracted from seeds, cotyledons, shoots, and young
leaves, depending on the germination energy of each seed, using magnetic
extraction method. Genetic analysis included eight accessions from Serbia (24
genotypes), two accessions from Montenegro (eight genotypes), and one
accession from Bosnia and Herzegovina and Croatia (with four genotypes each).
Accession-specific polymorphism was found at the Ol12-FO2 and Ol10-F11a
loci, where genotypes were qualitatively distinguished by horizontal agarose gel
electrophoresis (binary detection). Among 12 loci, the average polymorphic
information content (PIC) was 0.71, with the marker Ol13-C12 proving to be the
78 Umićević et al.
most informative (PIC = 0.89). However, the analysis of molecular variability
(AMOVA) showed 11% diversity between accessions, 19% diversity between
genotypes, and 70% diversity within genotypes, explaining the molecular
diversity of native Balkan leafy kale germplasm. According to the genetic
structure, four genetic groups were formed with an average expected
heterozygosity of 0.70 between clusters. In order to exploit the genetic diversity,
it would be advisable to evaluate these accessions at phenotypic level and use
their potential in breeding programmes., Raštan (Brassica oleracea var. acephala L.) je jedna od najvažnijih vrsta iz porodice kupusnjača koja povoljno utiče na zdravlje ljudi i odlikuje se velikom raznovrsnošću. Da bi se istražila varijabilnost unutar i između prinova, 12 prinova (svaka prinova se sastojala od četiri individue/genotipa) sa teritorije Balkanskog poluostrva je genotipizovano koristeći 12 SSR markera. Odabrani SSR markeri potiču iz genoma B. oleracea (osam), B. rapa (dva) i B. napus (dva). DNK je izolovana iz sjemena, kotiledona, izdanaka i mladih listova, u zavisnosti od energije klijanja svakog sjemena, metodom magnetne ekstrakcije. Genetičkom analizom obuhvaćeno je osam prinova iz Srbije (24 genotipa), dvije prinove iz Crne Gore (osam genotipova) i jedna prinova iz Bosne i Hercegovine i Hrvatske (sa po četiri genotipa). Polimorfizam specifičan za prinove pronađen je na lokusima Ol12-FO2 i Ol10-F11a, gdje su genotipovi kvalitativno razlikovani horizontalnom elektroforezom u agaroznom gelu (binarna detekcija). Kod ukupno 12 lokusa, prosječan sadržaj polimorfne informacije (PIC) bio je 0,71, pri čemu se kao najinformativniji pokazao marker Ol13-C12 (PIC = 0,89). Međutim, analiza molekularne varijabilnosti (AMOVA) pokazala je 11% raznovrsnosti između prinova, 19% raznovrsnosti između genotipova i 70% raznovrsnosti unutar genotipova, objašnjavajući molekularnu raznovrsnost izvorne germplazme raštana sa teritorije Balkanskog poluostrva. Prema genetičkoj strukturi formirane su četiri genetičke grupe sa prosječnom očekivanom heterozigotnošću od 0,70 između klastera. Da bi se iskoristio genetički diverzitet, bilo bi preporučljivo da se ove prinove karakterišu 90 Umićević et al. na fenotipskom nivou i da se njihov potencijal iskoristi u programima oplemenjivanja.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Agroznanje / Agro-knowledge Journal",
title = "Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.), Genetički diverzitet genetičkih resursa raštana (Brassica oleracea var. acephala L.)",
pages = "90-77",
number = "3",
volume = "24",
doi = "10.7251/AGREN2303077U"
}

Umićević, S., Antić, M., Rajković, D., Sinkovič, L., Nagl, N., Goreta Ban, S., Meglič, V., Todorović, V., Marjanović-Jeromela, A.,& Pipan, B.. (2023). Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.). in Agroznanje / Agro-knowledge Journal
Banja Luka : University of Banja Luka, Faculty of Agriculture., 24(3), 77-90.
https://doi.org/10.7251/AGREN2303077U

Umićević S, Antić M, Rajković D, Sinkovič L, Nagl N, Goreta Ban S, Meglič V, Todorović V, Marjanović-Jeromela A, Pipan B. Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.). in Agroznanje / Agro-knowledge Journal. 2023;24(3):77-90.
doi:10.7251/AGREN2303077U .

Umićević, Sonja, Antić, Marina, Rajković, Dragana, Sinkovič, Lovro, Nagl, Nevena, Goreta Ban, Smiljana, Meglič, Vladimir, Todorović, Vida, Marjanović-Jeromela, Ana, Pipan, Barbara, "Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.)" in Agroznanje / Agro-knowledge Journal, 24, no. 3 (2023):77-90,
https://doi.org/10.7251/AGREN2303077U . .

TY  - JOUR
AU  - Nagl, Nevena
AU  - Sinkovič, Lovro
AU  - Savić, Aleksandra
AU  - Isakov, Milada
AU  - Hasanaklou, Hourieh Tavakoli
AU  - Pipan, Barbara
AU  - Marjanović-Jeromela, Ana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3788
AB  - Grass pea (Lathyrus sativus) is a valuable grain legume known for its high protein content and rich essential amino acid profile. Its exceptional characteristics such as drought tolerance, high adaptability to extreme conditions, disease resistance, and low cultivation inputs, make it particularly suitable for cultivation by resource-poor farmers. However, the potential use of grass pea is limited due to the presence of anti-nutritional factors, including protease inhibitors, especially trypsin inhibitor. This study aimed to develop a rapid and reliable method for measuring TI activity in seeds of grass pea and to investigate the influence of genotype and environment on trypsin inhibitor (TI) activity in seeds of grass pea. A set of 25 grass pea accessions from seven European countries was cultivated in Slovenia at the Agricultural Institute of Slovenia, and a set of 12 grass pea accessions from the Serbian gene bank was grown in Serbia at the Institute of Field and Vegetable Crops. The TI activity varied considerably among the grass pea accessions studied, with values ranging from 26.7 to 90.3 TUI/mg. To further evaluate the effects of environmental conditions on TI activity, eight grass pea accessions originating from Serbia were grown in both Slovenia and Serbia. The TI activity of the accessions grown in Slovenia ranged from 26.7 to 81.0 TUI/mg, while the activity of the accessions grown in Serbia ranged from 40.3 to 57.0 TUI/mg. The correlation of TI activity between grass pea accessions grown in Slovenia and those grown in Serbia was 0.39, with genotype diversity being the largest contributor (55.9%). This study provides a valuable insight into the variability of TI activity in grass pea and shows the possible influence of environmental conditions on this trait. However, since the data are only from a one-year field trial, further studies are needed to fully evaluate the influence of different environmental factors on TI activity.
PB  - Novi Sad : Institute of Field and Vegetable Crops
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.)
EP  - 39
IS  - 2
SP  - 32
VL  - 60
DO  - 10.5937/ratpov60-45934
ER  - 

@article{
author = "Nagl, Nevena and Sinkovič, Lovro and Savić, Aleksandra and Isakov, Milada and Hasanaklou, Hourieh Tavakoli and Pipan, Barbara and Marjanović-Jeromela, Ana",
year = "2023",
abstract = "Grass pea (Lathyrus sativus) is a valuable grain legume known for its high protein content and rich essential amino acid profile. Its exceptional characteristics such as drought tolerance, high adaptability to extreme conditions, disease resistance, and low cultivation inputs, make it particularly suitable for cultivation by resource-poor farmers. However, the potential use of grass pea is limited due to the presence of anti-nutritional factors, including protease inhibitors, especially trypsin inhibitor. This study aimed to develop a rapid and reliable method for measuring TI activity in seeds of grass pea and to investigate the influence of genotype and environment on trypsin inhibitor (TI) activity in seeds of grass pea. A set of 25 grass pea accessions from seven European countries was cultivated in Slovenia at the Agricultural Institute of Slovenia, and a set of 12 grass pea accessions from the Serbian gene bank was grown in Serbia at the Institute of Field and Vegetable Crops. The TI activity varied considerably among the grass pea accessions studied, with values ranging from 26.7 to 90.3 TUI/mg. To further evaluate the effects of environmental conditions on TI activity, eight grass pea accessions originating from Serbia were grown in both Slovenia and Serbia. The TI activity of the accessions grown in Slovenia ranged from 26.7 to 81.0 TUI/mg, while the activity of the accessions grown in Serbia ranged from 40.3 to 57.0 TUI/mg. The correlation of TI activity between grass pea accessions grown in Slovenia and those grown in Serbia was 0.39, with genotype diversity being the largest contributor (55.9%). This study provides a valuable insight into the variability of TI activity in grass pea and shows the possible influence of environmental conditions on this trait. However, since the data are only from a one-year field trial, further studies are needed to fully evaluate the influence of different environmental factors on TI activity.",
publisher = "Novi Sad : Institute of Field and Vegetable Crops",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.)",
pages = "39-32",
number = "2",
volume = "60",
doi = "10.5937/ratpov60-45934"
}

Nagl, N., Sinkovič, L., Savić, A., Isakov, M., Hasanaklou, H. T., Pipan, B.,& Marjanović-Jeromela, A.. (2023). Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.). in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Novi Sad : Institute of Field and Vegetable Crops., 60(2), 32-39.
https://doi.org/10.5937/ratpov60-45934

Nagl N, Sinkovič L, Savić A, Isakov M, Hasanaklou HT, Pipan B, Marjanović-Jeromela A. Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.). in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2023;60(2):32-39.
doi:10.5937/ratpov60-45934 .

Nagl, Nevena, Sinkovič, Lovro, Savić, Aleksandra, Isakov, Milada, Hasanaklou, Hourieh Tavakoli, Pipan, Barbara, Marjanović-Jeromela, Ana, "Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.)" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 60, no. 2 (2023):32-39,
https://doi.org/10.5937/ratpov60-45934 . .

TY  - CONF
AU  - Nagl, Nevena
AU  - Sinkovič, Lovro
AU  - Vasić, Mirjana
AU  - Meglič, Vladimir
AU  - Savić, Aleksandra
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Pipan, Barbara
AU  - Marjanović-Jeromela, Ana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2804
AB  - Grass pea (Lathyrus sativus L.) is a legume with high protein content and good quantities of essential amino acids. It has high tolerance to drought, high adaptability to extreme conditions, disease resistance and low input requirement for cultivation, which makes it very useful for cultivation by resource-poor farmers. Potential usage of grass pea is limited due to the presence of anti-nutritional factors including protease inhibitors, especially trypsin inhibitors. They have a major impact on nutritional value, by inhibiting pancreatic serine proteases (thus impairing protein digestion), but are also likely to protect legume seed against attack by predators. Although once extensively grown throughout Balkan Peninsula, grass pea was nearly forgotten. In recent years there was an effort to reintroduce and increase the interest of researchers and farmers in this crop. The aim of this study was to establish whether genotype and environment have significant influences on trypsin inhibitor activity (TIA) in grass pea and to determine whether TIA is correlated with seed protein content.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
T1  - Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity
EP  - 82
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2804
ER  - 

@conference{
author = "Nagl, Nevena and Sinkovič, Lovro and Vasić, Mirjana and Meglič, Vladimir and Savić, Aleksandra and Nikolić, Zorica and Petrović, Gordana and Pipan, Barbara and Marjanović-Jeromela, Ana",
year = "2022",
abstract = "Grass pea (Lathyrus sativus L.) is a legume with high protein content and good quantities of essential amino acids. It has high tolerance to drought, high adaptability to extreme conditions, disease resistance and low input requirement for cultivation, which makes it very useful for cultivation by resource-poor farmers. Potential usage of grass pea is limited due to the presence of anti-nutritional factors including protease inhibitors, especially trypsin inhibitors. They have a major impact on nutritional value, by inhibiting pancreatic serine proteases (thus impairing protein digestion), but are also likely to protect legume seed against attack by predators. Although once extensively grown throughout Balkan Peninsula, grass pea was nearly forgotten. In recent years there was an effort to reintroduce and increase the interest of researchers and farmers in this crop. The aim of this study was to establish whether genotype and environment have significant influences on trypsin inhibitor activity (TIA) in grass pea and to determine whether TIA is correlated with seed protein content.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina",
title = "Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2804"
}

Nagl, N., Sinkovič, L., Vasić, M., Meglič, V., Savić, A., Nikolić, Z., Petrović, G., Pipan, B.,& Marjanović-Jeromela, A.. (2022). Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 82-82.
https://hdl.handle.net/21.15107/rcub_fiver_2804

Nagl N, Sinkovič L, Vasić M, Meglič V, Savić A, Nikolić Z, Petrović G, Pipan B, Marjanović-Jeromela A. Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina. 2022;:82-82.
https://hdl.handle.net/21.15107/rcub_fiver_2804 .

Nagl, Nevena, Sinkovič, Lovro, Vasić, Mirjana, Meglič, Vladimir, Savić, Aleksandra, Nikolić, Zorica, Petrović, Gordana, Pipan, Barbara, Marjanović-Jeromela, Ana, "Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity" in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina (2022):82-82,
https://hdl.handle.net/21.15107/rcub_fiver_2804 .

TY  - CONF
AU  - Pipan, Barbara
AU  - Antić, Marina
AU  - Rajković, Dragana
AU  - Sinkovič, Lovro
AU  - Rašeta, Sonja
AU  - Nagl, Nevena
AU  - Goreta Ban, Smiljana
AU  - Meglič, Vladimir
AU  - Todorović, Vida
AU  - Marjanović-Jeromela, Ana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2805
AB  - Family Brassicaceae is abundant in species used in food production, but also contains many industrial species, vegetables and ornamentals. Green collard (Brassica oleracea var. acephala L.) was traditionally grown in Balkan Peninsula and its wide green leaves, rich in vitamins A and C, are used for cooking. There is great diversity in B. oleracea acephala germplasm that reflects on agronomic, morphological and molecular characteristics. In this study, was assessed genetic variation of 38 accessions of green collard out of which 3 accessions were standard/commercial varieties and 35 were locally grown populations, collected in Western Balkan: 18 from Bosnia and Herzegovina, 8 from Serbia (Vojvodina), 7 from Croatia and two from Montenegro. Genetic diversity was assessed using twelve species-specific SSR markers (Simple Sequence Repeats) originating from B. oleracea (8), B. rapa (2) and B. napus (2). Each accession was represented with four individual samples.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
T1  - Assessment of molecular diversity of Brassica oleracea var. acephala
EP  - 138
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2805
ER  - 

@conference{
author = "Pipan, Barbara and Antić, Marina and Rajković, Dragana and Sinkovič, Lovro and Rašeta, Sonja and Nagl, Nevena and Goreta Ban, Smiljana and Meglič, Vladimir and Todorović, Vida and Marjanović-Jeromela, Ana",
year = "2022",
abstract = "Family Brassicaceae is abundant in species used in food production, but also contains many industrial species, vegetables and ornamentals. Green collard (Brassica oleracea var. acephala L.) was traditionally grown in Balkan Peninsula and its wide green leaves, rich in vitamins A and C, are used for cooking. There is great diversity in B. oleracea acephala germplasm that reflects on agronomic, morphological and molecular characteristics. In this study, was assessed genetic variation of 38 accessions of green collard out of which 3 accessions were standard/commercial varieties and 35 were locally grown populations, collected in Western Balkan: 18 from Bosnia and Herzegovina, 8 from Serbia (Vojvodina), 7 from Croatia and two from Montenegro. Genetic diversity was assessed using twelve species-specific SSR markers (Simple Sequence Repeats) originating from B. oleracea (8), B. rapa (2) and B. napus (2). Each accession was represented with four individual samples.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina",
title = "Assessment of molecular diversity of Brassica oleracea var. acephala",
pages = "138-137",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2805"
}

Pipan, B., Antić, M., Rajković, D., Sinkovič, L., Rašeta, S., Nagl, N., Goreta Ban, S., Meglič, V., Todorović, V.,& Marjanović-Jeromela, A.. (2022). Assessment of molecular diversity of Brassica oleracea var. acephala. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 137-138.
https://hdl.handle.net/21.15107/rcub_fiver_2805

Pipan B, Antić M, Rajković D, Sinkovič L, Rašeta S, Nagl N, Goreta Ban S, Meglič V, Todorović V, Marjanović-Jeromela A. Assessment of molecular diversity of Brassica oleracea var. acephala. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina. 2022;:137-138.
https://hdl.handle.net/21.15107/rcub_fiver_2805 .

Pipan, Barbara, Antić, Marina, Rajković, Dragana, Sinkovič, Lovro, Rašeta, Sonja, Nagl, Nevena, Goreta Ban, Smiljana, Meglič, Vladimir, Todorović, Vida, Marjanović-Jeromela, Ana, "Assessment of molecular diversity of Brassica oleracea var. acephala" in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina (2022):137-138,
https://hdl.handle.net/21.15107/rcub_fiver_2805 .

TY  - JOUR
AU  - Savić, Aleksandra
AU  - Pipan, Barbara
AU  - Vasić, Mirjana
AU  - Meglič, Vladimir
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2558
AB  - Genetic diversity and structure of common bean (Phaseolus vulgaris L.) germplasm from Serbia, comprising 118 landraces and 18 cultivars, was assessed with the application of 27 Single Sequence Repeats (SSR) markers. Thirteen accessions from Agricultural Institute of Slovenia were used as references for gene pool determination. Main parameters of genetic diversity were calculated for each SSR loci, i.e. number of different and rare alleles, number of effective alleles, Shannon’s information index, observed and expected heterozygosity and polymorphic information content. A total of 445 allelic variants, with 16.5 alleles per locus on average, were detected. Mean gene diversity (He = 0.79) indicated sufficient reservoir of genetic variation preserved in studied bean germplasm. Landraces displayed higher variability compared to cultivars (405 in relation to 233 allelic variants). Genetic structure and relatedness of accessions was assessed by model-based method and hierarchical clustering method in combination with genetic distance calculation. The Bayesian clustering model implemented in STRUCTURE software, on the primary level (K = 2), revealed clear separation of accessions into two groups, corresponding to gene pool affiliation. Mesoamerican gene pool (M) was represented with 23.5% of accessions, while Andean (A) was larger, composed of 68.4% of studied germplasm. Small group (8.1%) showed admixed genetic structure between two gene pools. Additional variation in respect to two recognized gene pools was revealed (K = 3), whose basis was acknowledged to be within Andean gene pool. Further subdivision of accessions (K = 8), mainly according to the seed forms, was observed. Genetic distance analysis associated with Neighbour-joining clustering method revealed grouping pattern of landraces and cultivars corresponding to the gene pool and their seed phenotypes. Classification and structuring of the bean accessions according to and beyond the gene pool of origin should facilitate conservation strategies and breeding of this material. Combining the information of phenotypic variation obtained in previous research and molecular data reveled in this study will assist in selection of parental components for breeding, or in the choice of smaller sample in order to further acknowledge their breeding value. In addition, obtained results of this work should serve as an additional information on common bean germplasm variation in Western Balkans and beyond, in Europe.
PB  - Elsevier
T2  - Scientia Horticulturae
T1  - Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR)
SP  - 110405
VL  - 288
DO  - 10.1016/j.scienta.2021.110405
ER  - 

@article{
author = "Savić, Aleksandra and Pipan, Barbara and Vasić, Mirjana and Meglič, Vladimir",
year = "2021",
abstract = "Genetic diversity and structure of common bean (Phaseolus vulgaris L.) germplasm from Serbia, comprising 118 landraces and 18 cultivars, was assessed with the application of 27 Single Sequence Repeats (SSR) markers. Thirteen accessions from Agricultural Institute of Slovenia were used as references for gene pool determination. Main parameters of genetic diversity were calculated for each SSR loci, i.e. number of different and rare alleles, number of effective alleles, Shannon’s information index, observed and expected heterozygosity and polymorphic information content. A total of 445 allelic variants, with 16.5 alleles per locus on average, were detected. Mean gene diversity (He = 0.79) indicated sufficient reservoir of genetic variation preserved in studied bean germplasm. Landraces displayed higher variability compared to cultivars (405 in relation to 233 allelic variants). Genetic structure and relatedness of accessions was assessed by model-based method and hierarchical clustering method in combination with genetic distance calculation. The Bayesian clustering model implemented in STRUCTURE software, on the primary level (K = 2), revealed clear separation of accessions into two groups, corresponding to gene pool affiliation. Mesoamerican gene pool (M) was represented with 23.5% of accessions, while Andean (A) was larger, composed of 68.4% of studied germplasm. Small group (8.1%) showed admixed genetic structure between two gene pools. Additional variation in respect to two recognized gene pools was revealed (K = 3), whose basis was acknowledged to be within Andean gene pool. Further subdivision of accessions (K = 8), mainly according to the seed forms, was observed. Genetic distance analysis associated with Neighbour-joining clustering method revealed grouping pattern of landraces and cultivars corresponding to the gene pool and their seed phenotypes. Classification and structuring of the bean accessions according to and beyond the gene pool of origin should facilitate conservation strategies and breeding of this material. Combining the information of phenotypic variation obtained in previous research and molecular data reveled in this study will assist in selection of parental components for breeding, or in the choice of smaller sample in order to further acknowledge their breeding value. In addition, obtained results of this work should serve as an additional information on common bean germplasm variation in Western Balkans and beyond, in Europe.",
publisher = "Elsevier",
journal = "Scientia Horticulturae",
title = "Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR)",
pages = "110405",
volume = "288",
doi = "10.1016/j.scienta.2021.110405"
}

Savić, A., Pipan, B., Vasić, M.,& Meglič, V.. (2021). Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR). in Scientia Horticulturae
Elsevier., 288, 110405.
https://doi.org/10.1016/j.scienta.2021.110405

Savić A, Pipan B, Vasić M, Meglič V. Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR). in Scientia Horticulturae. 2021;288:110405.
doi:10.1016/j.scienta.2021.110405 .

Savić, Aleksandra, Pipan, Barbara, Vasić, Mirjana, Meglič, Vladimir, "Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR)" in Scientia Horticulturae, 288 (2021):110405,
https://doi.org/10.1016/j.scienta.2021.110405 . .

TY  - JOUR
AU  - Savić, Aleksandra
AU  - Pipan, Barbara
AU  - Vasić, Mirjana
AU  - Meglič, Vladimir
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2559
AB  - Genetic diversity and structure of common bean (Phaseolus vulgaris L.) germplasm from Serbia, comprising 118 landraces and 18 cultivars, was assessed with the application of 27 Single Sequence Repeats (SSR) markers. Thirteen accessions from Agricultural Institute of Slovenia were used as references for gene pool determination. Main parameters of genetic diversity were calculated for each SSR loci, i.e. number of different and rare alleles, number of effective alleles, Shannon’s information index, observed and expected heterozygosity and polymorphic information content. A total of 445 allelic variants, with 16.5 alleles per locus on average, were detected. Mean gene diversity (He = 0.79) indicated sufficient reservoir of genetic variation preserved in studied bean germplasm. Landraces displayed higher variability compared to cultivars (405 in relation to 233 allelic variants). Genetic structure and relatedness of accessions was assessed by model-based method and hierarchical clustering method in combination with genetic distance calculation. The Bayesian clustering model implemented in STRUCTURE software, on the primary level (K = 2), revealed clear separation of accessions into two groups, corresponding to gene pool affiliation. Mesoamerican gene pool (M) was represented with 23.5% of accessions, while Andean (A) was larger, composed of 68.4% of studied germplasm. Small group (8.1%) showed admixed genetic structure between two gene pools. Additional variation in respect to two recognized gene pools was revealed (K = 3), whose basis was acknowledged to be within Andean gene pool. Further subdivision of accessions (K = 8), mainly according to the seed forms, was observed. Genetic distance analysis associated with Neighbour-joining clustering method revealed grouping pattern of landraces and cultivars corresponding to the gene pool and their seed phenotypes. Classification and structuring of the bean accessions according to and beyond the gene pool of origin should facilitate conservation strategies and breeding of this material. Combining the information of phenotypic variation obtained in previous research and molecular data reveled in this study will assist in selection of parental components for breeding, or in the choice of smaller sample in order to further acknowledge their breeding value. In addition, obtained results of this work should serve as an additional information on common bean germplasm variation in Western Balkans and beyond, in Europe.
PB  - Elsevier
T2  - Scientia Horticulturae
T1  - Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR)
SP  - 110405
VL  - 288
DO  - 10.1016/j.scienta.2021.110405
ER  - 

@article{
author = "Savić, Aleksandra and Pipan, Barbara and Vasić, Mirjana and Meglič, Vladimir",
year = "2021",
abstract = "Genetic diversity and structure of common bean (Phaseolus vulgaris L.) germplasm from Serbia, comprising 118 landraces and 18 cultivars, was assessed with the application of 27 Single Sequence Repeats (SSR) markers. Thirteen accessions from Agricultural Institute of Slovenia were used as references for gene pool determination. Main parameters of genetic diversity were calculated for each SSR loci, i.e. number of different and rare alleles, number of effective alleles, Shannon’s information index, observed and expected heterozygosity and polymorphic information content. A total of 445 allelic variants, with 16.5 alleles per locus on average, were detected. Mean gene diversity (He = 0.79) indicated sufficient reservoir of genetic variation preserved in studied bean germplasm. Landraces displayed higher variability compared to cultivars (405 in relation to 233 allelic variants). Genetic structure and relatedness of accessions was assessed by model-based method and hierarchical clustering method in combination with genetic distance calculation. The Bayesian clustering model implemented in STRUCTURE software, on the primary level (K = 2), revealed clear separation of accessions into two groups, corresponding to gene pool affiliation. Mesoamerican gene pool (M) was represented with 23.5% of accessions, while Andean (A) was larger, composed of 68.4% of studied germplasm. Small group (8.1%) showed admixed genetic structure between two gene pools. Additional variation in respect to two recognized gene pools was revealed (K = 3), whose basis was acknowledged to be within Andean gene pool. Further subdivision of accessions (K = 8), mainly according to the seed forms, was observed. Genetic distance analysis associated with Neighbour-joining clustering method revealed grouping pattern of landraces and cultivars corresponding to the gene pool and their seed phenotypes. Classification and structuring of the bean accessions according to and beyond the gene pool of origin should facilitate conservation strategies and breeding of this material. Combining the information of phenotypic variation obtained in previous research and molecular data reveled in this study will assist in selection of parental components for breeding, or in the choice of smaller sample in order to further acknowledge their breeding value. In addition, obtained results of this work should serve as an additional information on common bean germplasm variation in Western Balkans and beyond, in Europe.",
publisher = "Elsevier",
journal = "Scientia Horticulturae",
title = "Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR)",
pages = "110405",
volume = "288",
doi = "10.1016/j.scienta.2021.110405"
}

Savić, A., Pipan, B., Vasić, M.,& Meglič, V.. (2021). Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR). in Scientia Horticulturae
Elsevier., 288, 110405.
https://doi.org/10.1016/j.scienta.2021.110405

Savić A, Pipan B, Vasić M, Meglič V. Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR). in Scientia Horticulturae. 2021;288:110405.
doi:10.1016/j.scienta.2021.110405 .

Savić, Aleksandra, Pipan, Barbara, Vasić, Mirjana, Meglič, Vladimir, "Genetic diversity of common bean (Phaseolus vulgaris L.) germplasm from Serbia, as revealed by single sequence repeats (SSR)" in Scientia Horticulturae, 288 (2021):110405,
https://doi.org/10.1016/j.scienta.2021.110405 . .

TY  - CONF
AU  - Sinković, Lovro
AU  - Pipan, Barbara
AU  - Savić, Aleksandra
AU  - Vasić, Mirjana
AU  - Meglič, Vladimir
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2365
AB  - Grass pea (Lathyrus sativus L.) is a less common and nearly forgotten crop in many
countries, including Slovenia and Serbia. In the present study, genetic analysis and
seed characteristics of the collection of 22 grass pea accessions from several
Southeast European plant gene banks were examined. The collection was produced
in an open-field experiment at two countries/locations, i.e. Slovenia/Ljubljana –
Jablje and Serbia/Novi Sad – Rimski Šančevi, during the 2019 growing season. For
analysis of genetic diversity, specific high polymorphic SSR markers were applied.
Initial screening at 12 species-specific SSR loci using binary data revealed a great
genetic diversity among the grass pea accessions within a collection and three genetic
groups were formed. Differences in seed size were measured using descriptors for
seed length, width and thickness, and 100-seed weight. Furthermore, several seed
colour characters using IBPGR descriptors were visually assessed. The average seed
length, width and thickness of the seeds in the collection were 9.0 mm, 8.3 mm and
5.4 mm, respectively, while 100-seed weight ranged considerably from 11.7 g to
38.9 g. The highest differences between growing location were seen for 100-seed
weight where the coefficient of variability reached 23.5%. The examined grass pea
accessions had less monochromatic (one colour) and more colourful seeds, with two
or three colours.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina
T1  - Genetic diversity of Lathyrus sativus L. collection and characteristics of seeds grown in Slovenia and Serbia
EP  - 39
SP  - 39
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2365
ER  - 

@conference{
author = "Sinković, Lovro and Pipan, Barbara and Savić, Aleksandra and Vasić, Mirjana and Meglič, Vladimir",
year = "2021",
abstract = "Grass pea (Lathyrus sativus L.) is a less common and nearly forgotten crop in many
countries, including Slovenia and Serbia. In the present study, genetic analysis and
seed characteristics of the collection of 22 grass pea accessions from several
Southeast European plant gene banks were examined. The collection was produced
in an open-field experiment at two countries/locations, i.e. Slovenia/Ljubljana –
Jablje and Serbia/Novi Sad – Rimski Šančevi, during the 2019 growing season. For
analysis of genetic diversity, specific high polymorphic SSR markers were applied.
Initial screening at 12 species-specific SSR loci using binary data revealed a great
genetic diversity among the grass pea accessions within a collection and three genetic
groups were formed. Differences in seed size were measured using descriptors for
seed length, width and thickness, and 100-seed weight. Furthermore, several seed
colour characters using IBPGR descriptors were visually assessed. The average seed
length, width and thickness of the seeds in the collection were 9.0 mm, 8.3 mm and
5.4 mm, respectively, while 100-seed weight ranged considerably from 11.7 g to
38.9 g. The highest differences between growing location were seen for 100-seed
weight where the coefficient of variability reached 23.5%. The examined grass pea
accessions had less monochromatic (one colour) and more colourful seeds, with two
or three colours.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina",
title = "Genetic diversity of Lathyrus sativus L. collection and characteristics of seeds grown in Slovenia and Serbia",
pages = "39-39",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2365"
}

Sinković, L., Pipan, B., Savić, A., Vasić, M.,& Meglič, V.. (2021). Genetic diversity of Lathyrus sativus L. collection and characteristics of seeds grown in Slovenia and Serbia. in Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 39-39.
https://hdl.handle.net/21.15107/rcub_fiver_2365

Sinković L, Pipan B, Savić A, Vasić M, Meglič V. Genetic diversity of Lathyrus sativus L. collection and characteristics of seeds grown in Slovenia and Serbia. in Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina. 2021;:39-39.
https://hdl.handle.net/21.15107/rcub_fiver_2365 .

Sinković, Lovro, Pipan, Barbara, Savić, Aleksandra, Vasić, Mirjana, Meglič, Vladimir, "Genetic diversity of Lathyrus sativus L. collection and characteristics of seeds grown in Slovenia and Serbia" in Book of Abstracts, 10th International Symposium on Agricultural Sciences “AgroReS 2021", 27-29 May 2021, Trebinje, Bosnia and Herzegovina (2021):39-39,
https://hdl.handle.net/21.15107/rcub_fiver_2365 .

TY  - CONF
AU  - Sinkovič, Lovro
AU  - Pipan, Barbara
AU  - Meglič, Vladimir
AU  - Petrova, Sofija
AU  - Antić, Marina
AU  - Bebeli, Penelope
AU  - Pinheiro de Carvalho, Miguel Ângelo
AU  - Savić, Aleksandra
AU  - Vasić, Mirjana
AU  - Brezeanu, Creola
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2376
AB  - The ECPGR (European Cooperative Programme for Plant Genetic Resources) Grain legumes working group cover a range of species, which have enormous benefits for environment, food security and as well as the breeding material. However, despite its huge potential lots of species, including Lathyrus sp., are not well represented in European collection and enough exploited for different purposes. As starting point of EUGrainLeg project the activities act in two directions: i) AEGIS progress and improved data on European grain legumes plant genetic resources, and ii) improvement of group strategy work – in view of subgroup establishment based on species interest, in order to facilitate experience and knowledge exchange. The task of establishing the European grain legume collection needs updates and improvements, taking in account the large number of species managed inside the group. The significant diversity of Lathyrus sp. exist, but there are difficulties/gaps in order to identify accessions as AEGIS accessions, recording to registered data.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina
T1  - Lathyrus diversity: available resources with relevance to crop improvement – progress reviews and relationship with AEGIS (EUGrainLeg)
EP  - 62
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2376
ER  - 

@conference{
author = "Sinkovič, Lovro and Pipan, Barbara and Meglič, Vladimir and Petrova, Sofija and Antić, Marina and Bebeli, Penelope and Pinheiro de Carvalho, Miguel Ângelo and Savić, Aleksandra and Vasić, Mirjana and Brezeanu, Creola",
year = "2020",
abstract = "The ECPGR (European Cooperative Programme for Plant Genetic Resources) Grain legumes working group cover a range of species, which have enormous benefits for environment, food security and as well as the breeding material. However, despite its huge potential lots of species, including Lathyrus sp., are not well represented in European collection and enough exploited for different purposes. As starting point of EUGrainLeg project the activities act in two directions: i) AEGIS progress and improved data on European grain legumes plant genetic resources, and ii) improvement of group strategy work – in view of subgroup establishment based on species interest, in order to facilitate experience and knowledge exchange. The task of establishing the European grain legume collection needs updates and improvements, taking in account the large number of species managed inside the group. The significant diversity of Lathyrus sp. exist, but there are difficulties/gaps in order to identify accessions as AEGIS accessions, recording to registered data.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina",
title = "Lathyrus diversity: available resources with relevance to crop improvement – progress reviews and relationship with AEGIS (EUGrainLeg)",
pages = "62-61",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2376"
}

Sinkovič, L., Pipan, B., Meglič, V., Petrova, S., Antić, M., Bebeli, P., Pinheiro de Carvalho, M. Â., Savić, A., Vasić, M.,& Brezeanu, C.. (2020). Lathyrus diversity: available resources with relevance to crop improvement – progress reviews and relationship with AEGIS (EUGrainLeg). in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 61-62.
https://hdl.handle.net/21.15107/rcub_fiver_2376

Sinkovič L, Pipan B, Meglič V, Petrova S, Antić M, Bebeli P, Pinheiro de Carvalho MÂ, Savić A, Vasić M, Brezeanu C. Lathyrus diversity: available resources with relevance to crop improvement – progress reviews and relationship with AEGIS (EUGrainLeg). in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina. 2020;:61-62.
https://hdl.handle.net/21.15107/rcub_fiver_2376 .

Sinkovič, Lovro, Pipan, Barbara, Meglič, Vladimir, Petrova, Sofija, Antić, Marina, Bebeli, Penelope, Pinheiro de Carvalho, Miguel Ângelo, Savić, Aleksandra, Vasić, Mirjana, Brezeanu, Creola, "Lathyrus diversity: available resources with relevance to crop improvement – progress reviews and relationship with AEGIS (EUGrainLeg)" in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina (2020):61-62,
https://hdl.handle.net/21.15107/rcub_fiver_2376 .

TY  - JOUR
AU  - Sinković, Lovro
AU  - Pipan, Barbara
AU  - Vasić, Mirjana
AU  - Antić, Marina
AU  - Todorović, Vida
AU  - Ivanovska, Sonja
AU  - Brezeanu, Creola
AU  - Sustar-Vozlić, Jelka
AU  - Meglić, Vladimir
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1883
AB  - In South-Eastern Europe, the majority of runner-bean (Phaseolus coccineus L.) production is based on local populations grown mainly in home gardens. The local runner-bean plants are well adapted to their specific growing conditions and microclimate agro-environments, and show great morpho-agronomic diversity. Here, 142 runner-bean accessions from the five South-Eastern European countries of Slovenia, Bosnia and Herzegovina, Serbia, North Macedonia and Romania were sown and cultivated in their respective countries and characterised using 28 quantitative and qualitative morpho-agronomic descriptors for Phaseolus spp. based on inflorescences, leaves, plants, pods and seeds. For each of these morpho-agronomic descriptors, the accessions can be classified into two or three specific groups. The highest correlations were observed within the fluorescence, seed and pod traits. The highest variability, at 76.39%, was between the different countries, representing different geographic origins, while the variability within the countries was 23.61%. Cluster analysis based on these collected morpho-agronomic data also classified the accessions into three groups according to genetic origins. The data obtained serve as useful genetic information for plant breeders for the breeding of new bean varieties for further studies of the morpho-agronomic traits of the runner bean.
PB  - Basel : MDPI
T2  - Sustainability
T1  - Morpho-Agronomic Characterisation of Runner Bean (Phaseolus coccineus L.) from South-Eastern Europe
IS  - 21
VL  - 11
DO  - 10.3390/su11216165
ER  - 

@article{
author = "Sinković, Lovro and Pipan, Barbara and Vasić, Mirjana and Antić, Marina and Todorović, Vida and Ivanovska, Sonja and Brezeanu, Creola and Sustar-Vozlić, Jelka and Meglić, Vladimir",
year = "2019",
abstract = "In South-Eastern Europe, the majority of runner-bean (Phaseolus coccineus L.) production is based on local populations grown mainly in home gardens. The local runner-bean plants are well adapted to their specific growing conditions and microclimate agro-environments, and show great morpho-agronomic diversity. Here, 142 runner-bean accessions from the five South-Eastern European countries of Slovenia, Bosnia and Herzegovina, Serbia, North Macedonia and Romania were sown and cultivated in their respective countries and characterised using 28 quantitative and qualitative morpho-agronomic descriptors for Phaseolus spp. based on inflorescences, leaves, plants, pods and seeds. For each of these morpho-agronomic descriptors, the accessions can be classified into two or three specific groups. The highest correlations were observed within the fluorescence, seed and pod traits. The highest variability, at 76.39%, was between the different countries, representing different geographic origins, while the variability within the countries was 23.61%. Cluster analysis based on these collected morpho-agronomic data also classified the accessions into three groups according to genetic origins. The data obtained serve as useful genetic information for plant breeders for the breeding of new bean varieties for further studies of the morpho-agronomic traits of the runner bean.",
publisher = "Basel : MDPI",
journal = "Sustainability",
title = "Morpho-Agronomic Characterisation of Runner Bean (Phaseolus coccineus L.) from South-Eastern Europe",
number = "21",
volume = "11",
doi = "10.3390/su11216165"
}

Sinković, L., Pipan, B., Vasić, M., Antić, M., Todorović, V., Ivanovska, S., Brezeanu, C., Sustar-Vozlić, J.,& Meglić, V.. (2019). Morpho-Agronomic Characterisation of Runner Bean (Phaseolus coccineus L.) from South-Eastern Europe. in Sustainability
Basel : MDPI., 11(21).
https://doi.org/10.3390/su11216165

Sinković L, Pipan B, Vasić M, Antić M, Todorović V, Ivanovska S, Brezeanu C, Sustar-Vozlić J, Meglić V. Morpho-Agronomic Characterisation of Runner Bean (Phaseolus coccineus L.) from South-Eastern Europe. in Sustainability. 2019;11(21).
doi:10.3390/su11216165 .

Sinković, Lovro, Pipan, Barbara, Vasić, Mirjana, Antić, Marina, Todorović, Vida, Ivanovska, Sonja, Brezeanu, Creola, Sustar-Vozlić, Jelka, Meglić, Vladimir, "Morpho-Agronomic Characterisation of Runner Bean (Phaseolus coccineus L.) from South-Eastern Europe" in Sustainability, 11, no. 21 (2019),
https://doi.org/10.3390/su11216165 . .

TY  - CONF
AU  - Pipan, Barbara
AU  - Šuštar-Vozlič, Jelka
AU  - Todorović, Vida
AU  - Antić, Marina
AU  - Creola, Brezeanu
AU  - Ivanovska, Sonja
AU  - Jankulovska, Mirjana
AU  - Savić, Aleksandra
AU  - Vasić, Mirjana
AU  - Meglič, Vladimir
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/3957
AB  - In the last decade several thousand accessions were collected in different parts of Europe and are stored in national gene banks; at present the Phaseolus Database as part of the EURISCO Web catalogue contains over 46000 records. Main objective of the study (ECPGR_SMARTLEG project) was proper phenotypic and genetic evaluation of European Phaseolus accessions with emphasis to Phaseolus coccineus germplasm providing new data to EURISCO and AEGIS. During the project, we provided three different types of datasets including morpho-agronomic traits of plants, morphometric seed characteristics and genetic profiles of P. coccineus accessions from eight European geographic origins; Slovenia, Romania, Bosnia and Herzegovina, Serbia, Italy, Slovakia, Macedonia and Austria. Before sowing, the morphometric characterization of P. coccineus seeds was performed according to adopted Community Plant Variety Office-Technical Protocol and Phaselieu/AIS descriptors observing 14 different seed characteristics.
PB  - Belgrade : University of Belgrade, Institute of Botany and 'Jevremovac' Botanical Garden
C3  - Book of Abstracts, 7th Balkan Botanical Congress (7BBC 2018), 10-14 September 2018, Novi Sad
T1  - Evaluation of the Balkan Phaseolus coccineus L. genetic resources
EP  - 167
SP  - 166
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3957
ER  - 

@conference{
author = "Pipan, Barbara and Šuštar-Vozlič, Jelka and Todorović, Vida and Antić, Marina and Creola, Brezeanu and Ivanovska, Sonja and Jankulovska, Mirjana and Savić, Aleksandra and Vasić, Mirjana and Meglič, Vladimir",
year = "2018",
abstract = "In the last decade several thousand accessions were collected in different parts of Europe and are stored in national gene banks; at present the Phaseolus Database as part of the EURISCO Web catalogue contains over 46000 records. Main objective of the study (ECPGR_SMARTLEG project) was proper phenotypic and genetic evaluation of European Phaseolus accessions with emphasis to Phaseolus coccineus germplasm providing new data to EURISCO and AEGIS. During the project, we provided three different types of datasets including morpho-agronomic traits of plants, morphometric seed characteristics and genetic profiles of P. coccineus accessions from eight European geographic origins; Slovenia, Romania, Bosnia and Herzegovina, Serbia, Italy, Slovakia, Macedonia and Austria. Before sowing, the morphometric characterization of P. coccineus seeds was performed according to adopted Community Plant Variety Office-Technical Protocol and Phaselieu/AIS descriptors observing 14 different seed characteristics.",
publisher = "Belgrade : University of Belgrade, Institute of Botany and 'Jevremovac' Botanical Garden",
journal = "Book of Abstracts, 7th Balkan Botanical Congress (7BBC 2018), 10-14 September 2018, Novi Sad",
title = "Evaluation of the Balkan Phaseolus coccineus L. genetic resources",
pages = "167-166",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3957"
}

Pipan, B., Šuštar-Vozlič, J., Todorović, V., Antić, M., Creola, B., Ivanovska, S., Jankulovska, M., Savić, A., Vasić, M.,& Meglič, V.. (2018). Evaluation of the Balkan Phaseolus coccineus L. genetic resources. in Book of Abstracts, 7th Balkan Botanical Congress (7BBC 2018), 10-14 September 2018, Novi Sad
Belgrade : University of Belgrade, Institute of Botany and 'Jevremovac' Botanical Garden., 42, 166-167.
https://hdl.handle.net/21.15107/rcub_fiver_3957

Pipan B, Šuštar-Vozlič J, Todorović V, Antić M, Creola B, Ivanovska S, Jankulovska M, Savić A, Vasić M, Meglič V. Evaluation of the Balkan Phaseolus coccineus L. genetic resources. in Book of Abstracts, 7th Balkan Botanical Congress (7BBC 2018), 10-14 September 2018, Novi Sad. 2018;42:166-167.
https://hdl.handle.net/21.15107/rcub_fiver_3957 .

Pipan, Barbara, Šuštar-Vozlič, Jelka, Todorović, Vida, Antić, Marina, Creola, Brezeanu, Ivanovska, Sonja, Jankulovska, Mirjana, Savić, Aleksandra, Vasić, Mirjana, Meglič, Vladimir, "Evaluation of the Balkan Phaseolus coccineus L. genetic resources" in Book of Abstracts, 7th Balkan Botanical Congress (7BBC 2018), 10-14 September 2018, Novi Sad, 42 (2018):166-167,
https://hdl.handle.net/21.15107/rcub_fiver_3957 .

TY  - CONF
AU  - Pipan, Barbara
AU  - Šuštar-Vozlič, Jelka
AU  - Todorović, Vida
AU  - Antić, Marina
AU  - Creola, Brezeanu
AU  - Ivanovska, Sonja
AU  - Jankulovska, Mirjana
AU  - Savić, Aleksandra
AU  - Vasić, Mirjana
AU  - Meglič, Vladimir
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2251
AB  - In the last decade several thousand accessions were collected in different parts of Europe and are stored in national gene banks; at present the Phaseolus Database as part of the EURISCO Web catalogue contains aver 46000 records. Main objective of the study (ECPGR_SMARTLEG project) was proper phenotypic and genetic evaluation of European Phaseolus accessions with emphasis to Phaseolus coccineus germplasm providing new data to EURISCO and AEGIS. During the project, we provided three different types of datasets including morpho-agronomic traits of plants, morphometric seed characteristics and genetic profiles of P. coccineus accessions from eight European geographic origins; Slovenia, Romania, Bosnia and Herzegovina, Serbia, Italy, Slovakia, Macedonia and Austria.
PB  - Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac
C3  - Book of Abstracts, 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia, Botanica Serbica 42 (supplement 1)
T1  - Evaluation of the Balkan Phaseolus coccineus L. genetic resources
EP  - 166
SP  - 166
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2251
ER  - 

@conference{
author = "Pipan, Barbara and Šuštar-Vozlič, Jelka and Todorović, Vida and Antić, Marina and Creola, Brezeanu and Ivanovska, Sonja and Jankulovska, Mirjana and Savić, Aleksandra and Vasić, Mirjana and Meglič, Vladimir",
year = "2018",
abstract = "In the last decade several thousand accessions were collected in different parts of Europe and are stored in national gene banks; at present the Phaseolus Database as part of the EURISCO Web catalogue contains aver 46000 records. Main objective of the study (ECPGR_SMARTLEG project) was proper phenotypic and genetic evaluation of European Phaseolus accessions with emphasis to Phaseolus coccineus germplasm providing new data to EURISCO and AEGIS. During the project, we provided three different types of datasets including morpho-agronomic traits of plants, morphometric seed characteristics and genetic profiles of P. coccineus accessions from eight European geographic origins; Slovenia, Romania, Bosnia and Herzegovina, Serbia, Italy, Slovakia, Macedonia and Austria.",
publisher = "Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac",
journal = "Book of Abstracts, 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia, Botanica Serbica 42 (supplement 1)",
title = "Evaluation of the Balkan Phaseolus coccineus L. genetic resources",
pages = "166-166",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2251"
}

Pipan, B., Šuštar-Vozlič, J., Todorović, V., Antić, M., Creola, B., Ivanovska, S., Jankulovska, M., Savić, A., Vasić, M.,& Meglič, V.. (2018). Evaluation of the Balkan Phaseolus coccineus L. genetic resources. in Book of Abstracts, 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia, Botanica Serbica 42 (supplement 1)
Belgrade : University of Belgrade, Institute of Botany and Botanical Garden Jevremovac., 166-166.
https://hdl.handle.net/21.15107/rcub_fiver_2251

Pipan B, Šuštar-Vozlič J, Todorović V, Antić M, Creola B, Ivanovska S, Jankulovska M, Savić A, Vasić M, Meglič V. Evaluation of the Balkan Phaseolus coccineus L. genetic resources. in Book of Abstracts, 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia, Botanica Serbica 42 (supplement 1). 2018;:166-166.
https://hdl.handle.net/21.15107/rcub_fiver_2251 .

Pipan, Barbara, Šuštar-Vozlič, Jelka, Todorović, Vida, Antić, Marina, Creola, Brezeanu, Ivanovska, Sonja, Jankulovska, Mirjana, Savić, Aleksandra, Vasić, Mirjana, Meglič, Vladimir, "Evaluation of the Balkan Phaseolus coccineus L. genetic resources" in Book of Abstracts, 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia, Botanica Serbica 42 (supplement 1) (2018):166-166,
https://hdl.handle.net/21.15107/rcub_fiver_2251 .

TY  - JOUR
AU  - Maras, Marko
AU  - Pipan, Barbara
AU  - Sustar-Vozlić, Jelka
AU  - Todorović, Vida
AU  - Durić, Gordana
AU  - Vasić, Mirjana
AU  - Kratovalieva, Suzana
AU  - Ibusoska, Afrodita
AU  - Agić, Rukie
AU  - Matotan, Zdravko
AU  - Cupić, Tihomir
AU  - Meglić, Vladimir
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1421
AB  - In this study, genetic diversity of 119 accessions of common bean (Phaseolus vulgaris) from five former Yugoslav republics constituting the western Balkans was assessed by 13 microsatellite markers. This set of markers has proven before to efficiently distinguish between bean genotypes and assign them to either the Andean or the Mesoamerican gene pool of origin. In this study, 118 alleles were detected or 9.1 per locus on average. Four groups (i.e., Slovene, Croatian, Bosnian, and Serbian) showed similarly high levels of genetic diversity as estimated by the number of different alleles, number of effective alleles, Shannon's information index, and expected heterozygosity. Mildly narrower genetic diversity was identified within a group of Macedonian accessions; however, this germplasm yielded the highest number of private alleles. All five germplasms share a great portion of genetic diversity as indicated by the analysis of molecular variance (AMOVA). On the basis of the scored number of migrants, we concluded that the most intensive gene flow in the region exists in Bosnia and Herzegovina. Cluster analysis based on collected molecular data classified the accessions into two large clusters that corresponded to two gene pools of origin (i.e., Andean and Mesoamerican). We found that Andean genotypes are more prevalent than Mesoamerican in all studied countries, except Macedonia, where the two gene pools are represented evenly. This could indicate that common bean was introduced into the western Balkans mainly from the Mediterranean Basin. Bayesian cluster analysis revealed that in the area studied an additional variation exists which is related to the Andean gene pool. Different scenarios of the origin of this variation are discussed in the article.
PB  - Amer Soc Horticultural Science, Alexandria
T2  - Journal of The American Society for Horticultural Science
T1  - Examination of Genetic Diversity of Common Bean from the Western Balkans
EP  - 316
IS  - 4
SP  - 308
VL  - 140
DO  - 10.21273/JASHS.140.4.308
ER  - 

@article{
author = "Maras, Marko and Pipan, Barbara and Sustar-Vozlić, Jelka and Todorović, Vida and Durić, Gordana and Vasić, Mirjana and Kratovalieva, Suzana and Ibusoska, Afrodita and Agić, Rukie and Matotan, Zdravko and Cupić, Tihomir and Meglić, Vladimir",
year = "2015",
abstract = "In this study, genetic diversity of 119 accessions of common bean (Phaseolus vulgaris) from five former Yugoslav republics constituting the western Balkans was assessed by 13 microsatellite markers. This set of markers has proven before to efficiently distinguish between bean genotypes and assign them to either the Andean or the Mesoamerican gene pool of origin. In this study, 118 alleles were detected or 9.1 per locus on average. Four groups (i.e., Slovene, Croatian, Bosnian, and Serbian) showed similarly high levels of genetic diversity as estimated by the number of different alleles, number of effective alleles, Shannon's information index, and expected heterozygosity. Mildly narrower genetic diversity was identified within a group of Macedonian accessions; however, this germplasm yielded the highest number of private alleles. All five germplasms share a great portion of genetic diversity as indicated by the analysis of molecular variance (AMOVA). On the basis of the scored number of migrants, we concluded that the most intensive gene flow in the region exists in Bosnia and Herzegovina. Cluster analysis based on collected molecular data classified the accessions into two large clusters that corresponded to two gene pools of origin (i.e., Andean and Mesoamerican). We found that Andean genotypes are more prevalent than Mesoamerican in all studied countries, except Macedonia, where the two gene pools are represented evenly. This could indicate that common bean was introduced into the western Balkans mainly from the Mediterranean Basin. Bayesian cluster analysis revealed that in the area studied an additional variation exists which is related to the Andean gene pool. Different scenarios of the origin of this variation are discussed in the article.",
publisher = "Amer Soc Horticultural Science, Alexandria",
journal = "Journal of The American Society for Horticultural Science",
title = "Examination of Genetic Diversity of Common Bean from the Western Balkans",
pages = "316-308",
number = "4",
volume = "140",
doi = "10.21273/JASHS.140.4.308"
}

Maras, M., Pipan, B., Sustar-Vozlić, J., Todorović, V., Durić, G., Vasić, M., Kratovalieva, S., Ibusoska, A., Agić, R., Matotan, Z., Cupić, T.,& Meglić, V.. (2015). Examination of Genetic Diversity of Common Bean from the Western Balkans. in Journal of The American Society for Horticultural Science
Amer Soc Horticultural Science, Alexandria., 140(4), 308-316.
https://doi.org/10.21273/JASHS.140.4.308

Maras M, Pipan B, Sustar-Vozlić J, Todorović V, Durić G, Vasić M, Kratovalieva S, Ibusoska A, Agić R, Matotan Z, Cupić T, Meglić V. Examination of Genetic Diversity of Common Bean from the Western Balkans. in Journal of The American Society for Horticultural Science. 2015;140(4):308-316.
doi:10.21273/JASHS.140.4.308 .

Maras, Marko, Pipan, Barbara, Sustar-Vozlić, Jelka, Todorović, Vida, Durić, Gordana, Vasić, Mirjana, Kratovalieva, Suzana, Ibusoska, Afrodita, Agić, Rukie, Matotan, Zdravko, Cupić, Tihomir, Meglić, Vladimir, "Examination of Genetic Diversity of Common Bean from the Western Balkans" in Journal of The American Society for Horticultural Science, 140, no. 4 (2015):308-316,
https://doi.org/10.21273/JASHS.140.4.308 . .

TY  - CONF
AU  - Meglič, Vladimir
AU  - Pipan, Barbara
AU  - Šuštar-Vozlič, Jelka
AU  - Maras, Marko
AU  - Todorović, Vida
AU  - Vasić, Mirjana
AU  - Kratovalieva, Suzana
AU  - Ibusoska, Afrodita
AU  - Matotan, Zdravko
AU  - Čupić, Tihomir
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2984
AB  - Genetic differentiation of 167 Phaseolus vulgaris L. accessions originating from five South east European countries was performed using 13 SSR markers. Analysis included 21 accessions from Bosnia and Herzegovina, 15 accessions from Croatia, 73 accessions from Macedonia, 38 accessions from Serbia and 20 accessions from Slovenia. Calculated mean number of alleles per locus was 9.8. The average polymorphic information content over all loci reached value of 0.710 where the most informative locus was GATS91 (0.926). These results showed that selected set of SSR markers are highly informative and applicable for studies of genetic diversity within germplasm collected in the five South east European countries, which was also confirmed with the calculated value of probability of identity (1.831x10). High mean value of expected heterozygosity (0.731) and Shannon’s information index (1.486) reflects high genetic diversity within accessions from five countries, where accessions from Macedonia are genetically the most uniform, due to 17completely identical accessions included.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Genetic diversity of the autochtonous Phaseolus bean germplasm originating from five South east European countries
EP  - 29
SP  - 29
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2984
ER  - 

@conference{
author = "Meglič, Vladimir and Pipan, Barbara and Šuštar-Vozlič, Jelka and Maras, Marko and Todorović, Vida and Vasić, Mirjana and Kratovalieva, Suzana and Ibusoska, Afrodita and Matotan, Zdravko and Čupić, Tihomir",
year = "2013",
abstract = "Genetic differentiation of 167 Phaseolus vulgaris L. accessions originating from five South east European countries was performed using 13 SSR markers. Analysis included 21 accessions from Bosnia and Herzegovina, 15 accessions from Croatia, 73 accessions from Macedonia, 38 accessions from Serbia and 20 accessions from Slovenia. Calculated mean number of alleles per locus was 9.8. The average polymorphic information content over all loci reached value of 0.710 where the most informative locus was GATS91 (0.926). These results showed that selected set of SSR markers are highly informative and applicable for studies of genetic diversity within germplasm collected in the five South east European countries, which was also confirmed with the calculated value of probability of identity (1.831x10). High mean value of expected heterozygosity (0.731) and Shannon’s information index (1.486) reflects high genetic diversity within accessions from five countries, where accessions from Macedonia are genetically the most uniform, due to 17completely identical accessions included.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Genetic diversity of the autochtonous Phaseolus bean germplasm originating from five South east European countries",
pages = "29-29",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2984"
}

Meglič, V., Pipan, B., Šuštar-Vozlič, J., Maras, M., Todorović, V., Vasić, M., Kratovalieva, S., Ibusoska, A., Matotan, Z.,& Čupić, T.. (2013). Genetic diversity of the autochtonous Phaseolus bean germplasm originating from five South east European countries. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 29-29.
https://hdl.handle.net/21.15107/rcub_fiver_2984

Meglič V, Pipan B, Šuštar-Vozlič J, Maras M, Todorović V, Vasić M, Kratovalieva S, Ibusoska A, Matotan Z, Čupić T. Genetic diversity of the autochtonous Phaseolus bean germplasm originating from five South east European countries. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:29-29.
https://hdl.handle.net/21.15107/rcub_fiver_2984 .

Meglič, Vladimir, Pipan, Barbara, Šuštar-Vozlič, Jelka, Maras, Marko, Todorović, Vida, Vasić, Mirjana, Kratovalieva, Suzana, Ibusoska, Afrodita, Matotan, Zdravko, Čupić, Tihomir, "Genetic diversity of the autochtonous Phaseolus bean germplasm originating from five South east European countries" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):29-29,
https://hdl.handle.net/21.15107/rcub_fiver_2984 .