TY  - JOUR
AU  - Petrović, Gordana
AU  - Nikolić, Zorica
AU  - Živanović, Tomislav
AU  - Vasiljević, Sanja
AU  - Milošević, Dragana
AU  - Stanisavljević, Nemanja
AU  - Samardžić, Jelena
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4106
AB  - Background: This study was carried out for an understanding of the antioxidant mechanisms of field pea varieties under osmotic stress conditions caused by a lack of water. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APx) and glutathione reductase (GR) were examined and analyzed. The gene expression levels of Cu/Zn SOD, cAPx and GR genes were also examined.
Methods: Osmotic stress was stimulated using PEG 6000 with the osmotic potential of -0.1 MPa in 10 days old plant seedlings. The activity of the antioxidant enzymes was measured in the shoots and roots of pea seedlings. The gene expression levels of genes coding antioxidative enzymes were examined by the reverse transcription polymerase chain reaction (RT-PCR) technique. Arabidopsis 18S rRNA was used as endogenous control.
Result: Osmotic stress changed the activities of antioxidant enzymes in the shoots and roots of pea seedlings. Varieties more tolerant to osmotic stress showed a significant increase in antioxidant activities in shoots and roots, while sensitive varieties showed a significant decrease. The results of the analysis of the expression of genes, coding for antioxidant enzymes, showed that the reaction of the tested cultivars to ROS was the result of increased expression of the tested genes in tolerant cultivars, i.e. decreased expression in sensitive cultivars.
PB  - Agricultural Research Communication Centre
T2  - Legume Research
T1  - Drought-induced Changes in the Antioxidant System in Pisum sativum L.
EP  - 1452
IS  - 11
SP  - 1445
VL  - 46
DO  - 10.18805/LRF-755
ER  - 

@article{
author = "Petrović, Gordana and Nikolić, Zorica and Živanović, Tomislav and Vasiljević, Sanja and Milošević, Dragana and Stanisavljević, Nemanja and Samardžić, Jelena",
year = "2023",
abstract = "Background: This study was carried out for an understanding of the antioxidant mechanisms of field pea varieties under osmotic stress conditions caused by a lack of water. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APx) and glutathione reductase (GR) were examined and analyzed. The gene expression levels of Cu/Zn SOD, cAPx and GR genes were also examined.
Methods: Osmotic stress was stimulated using PEG 6000 with the osmotic potential of -0.1 MPa in 10 days old plant seedlings. The activity of the antioxidant enzymes was measured in the shoots and roots of pea seedlings. The gene expression levels of genes coding antioxidative enzymes were examined by the reverse transcription polymerase chain reaction (RT-PCR) technique. Arabidopsis 18S rRNA was used as endogenous control.
Result: Osmotic stress changed the activities of antioxidant enzymes in the shoots and roots of pea seedlings. Varieties more tolerant to osmotic stress showed a significant increase in antioxidant activities in shoots and roots, while sensitive varieties showed a significant decrease. The results of the analysis of the expression of genes, coding for antioxidant enzymes, showed that the reaction of the tested cultivars to ROS was the result of increased expression of the tested genes in tolerant cultivars, i.e. decreased expression in sensitive cultivars.",
publisher = "Agricultural Research Communication Centre",
journal = "Legume Research",
title = "Drought-induced Changes in the Antioxidant System in Pisum sativum L.",
pages = "1452-1445",
number = "11",
volume = "46",
doi = "10.18805/LRF-755"
}

Petrović, G., Nikolić, Z., Živanović, T., Vasiljević, S., Milošević, D., Stanisavljević, N.,& Samardžić, J.. (2023). Drought-induced Changes in the Antioxidant System in Pisum sativum L.. in Legume Research
Agricultural Research Communication Centre., 46(11), 1445-1452.
https://doi.org/10.18805/LRF-755

Petrović G, Nikolić Z, Živanović T, Vasiljević S, Milošević D, Stanisavljević N, Samardžić J. Drought-induced Changes in the Antioxidant System in Pisum sativum L.. in Legume Research. 2023;46(11):1445-1452.
doi:10.18805/LRF-755 .

Petrović, Gordana, Nikolić, Zorica, Živanović, Tomislav, Vasiljević, Sanja, Milošević, Dragana, Stanisavljević, Nemanja, Samardžić, Jelena, "Drought-induced Changes in the Antioxidant System in Pisum sativum L." in Legume Research, 46, no. 11 (2023):1445-1452,
https://doi.org/10.18805/LRF-755 . .

TY  - CONF
AU  - Miladinović, Dragana
AU  - Čitaković, Ivan
AU  - Dedić, Boško
AU  - Cvejić, Sandra
AU  - Radanović, Aleksandra
AU  - Jocić, Siniša
AU  - Jocković, Milan
AU  - Marjanović-Jeromela, Ana
AU  - Samardžić, Jelena
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/2303
AB  - During the evolution plants have developed a large set af defense mechanisms aerinetpashogens, Defense responses have been sudlein dealin model lant speces such as Arabidopsis. However, to complete our itis i to study defense in del plants, Suhoe In our experiment we aimed to study expression of defense-related genes in the sunflower host plant during combined Infection with downy mildew and broomrape and elucidate thelr role in sunflower reaction to attack of different pathogens.  Downey-mildew-resistant genotype was inoculated with the pathogen and planted Into the broomrape inoculated soll. Far gene expression analysis, leaf samples were taken at different stages from planis with combined broomrape-downy mildew infection, plants only infected with broomrape, and control plants, Expression of defense related genes is analyzed using RT-PCR, with special focus on defensin.
PB  - Brussels : COST Association
C3  - Book of Abstracts, Cost INDEPTH meeting, 24-27 February 2019, Prague, Czech Republic
T1  - Sunflower reaction to combined broomrape – downy mildew attack, Preliminary study
EP  - 92
SP  - 92
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2303
ER  - 

@conference{
author = "Miladinović, Dragana and Čitaković, Ivan and Dedić, Boško and Cvejić, Sandra and Radanović, Aleksandra and Jocić, Siniša and Jocković, Milan and Marjanović-Jeromela, Ana and Samardžić, Jelena",
year = "2019",
abstract = "During the evolution plants have developed a large set af defense mechanisms aerinetpashogens, Defense responses have been sudlein dealin model lant speces such as Arabidopsis. However, to complete our itis i to study defense in del plants, Suhoe In our experiment we aimed to study expression of defense-related genes in the sunflower host plant during combined Infection with downy mildew and broomrape and elucidate thelr role in sunflower reaction to attack of different pathogens.  Downey-mildew-resistant genotype was inoculated with the pathogen and planted Into the broomrape inoculated soll. Far gene expression analysis, leaf samples were taken at different stages from planis with combined broomrape-downy mildew infection, plants only infected with broomrape, and control plants, Expression of defense related genes is analyzed using RT-PCR, with special focus on defensin.",
publisher = "Brussels : COST Association",
journal = "Book of Abstracts, Cost INDEPTH meeting, 24-27 February 2019, Prague, Czech Republic",
title = "Sunflower reaction to combined broomrape – downy mildew attack, Preliminary study",
pages = "92-92",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2303"
}

Miladinović, D., Čitaković, I., Dedić, B., Cvejić, S., Radanović, A., Jocić, S., Jocković, M., Marjanović-Jeromela, A.,& Samardžić, J.. (2019). Sunflower reaction to combined broomrape – downy mildew attack, Preliminary study. in Book of Abstracts, Cost INDEPTH meeting, 24-27 February 2019, Prague, Czech Republic
Brussels : COST Association., 92-92.
https://hdl.handle.net/21.15107/rcub_fiver_2303

Miladinović D, Čitaković I, Dedić B, Cvejić S, Radanović A, Jocić S, Jocković M, Marjanović-Jeromela A, Samardžić J. Sunflower reaction to combined broomrape – downy mildew attack, Preliminary study. in Book of Abstracts, Cost INDEPTH meeting, 24-27 February 2019, Prague, Czech Republic. 2019;:92-92.
https://hdl.handle.net/21.15107/rcub_fiver_2303 .

Miladinović, Dragana, Čitaković, Ivan, Dedić, Boško, Cvejić, Sandra, Radanović, Aleksandra, Jocić, Siniša, Jocković, Milan, Marjanović-Jeromela, Ana, Samardžić, Jelena, "Sunflower reaction to combined broomrape – downy mildew attack, Preliminary study" in Book of Abstracts, Cost INDEPTH meeting, 24-27 February 2019, Prague, Czech Republic (2019):92-92,
https://hdl.handle.net/21.15107/rcub_fiver_2303 .

TY  - CONF
AU  - Petrović, Gordana
AU  - Stikić, Radmila
AU  - Živanović, Tomislav
AU  - Nikolić, Zorica
AU  - Marjanović, Milena
AU  - Petrović, Ivana
AU  - Samardžić, Jelena
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/3089
AB  - Izlaganje stresnim uslovima životne sredine, kao što je suša, ima negativan efekat na rast biljaka. Abcisinska kiselina (ABA) se akumulira u uslovima osmotskog stresa i igra važnu ulogu u odgovoru na stres i u toleranciji biljaka. Cilj ovog istraživanja je bio da se odredi koncentracija ABA i ekspresija aldehid oksidaznog enzima (AO) kod 7 sorti stočnog graška u početnim fazama klijanja semena i u stadijumu trećeg para listova. Osmotski stres je stimulisan primenom PEG 6000. Stres suše je izazvan prekidanjem zalivanja. Koncentracija ABA je merena Elisa metodom. U nadzemnom delu ponika utvrđeno je značajno povećanje sadržaja ABA kod jedne grupe sorti (Javor, Partner, Junior) i smanjenje koncentracije ABA kod drugih sorti (Mraz, Dukat, Pionir, Trezor).
AB  - Exposure to to environmental stresses, such as drought, causes adverse effects on the growth of plants. Abscisic acid (ABA) accumulates under osmotic stress conditions and plays an important role in the stress response and tolerance of plants. The aim of this research was to determine the rates of ABA and the expression of Aldehyde oxidase enzyme (AO) among 7 cultivars of field pea at early seedling stage and at stage of third pair of leaves. Osmotic stress was stimulated using PEG 6000. The drought stress was induced by a termination of irrigation. ABA concentration was measured by the Elisa method. In the shoot of seedlings a significant increase in ABA content in one group of cultivars (Javor, Partner, Junior), and a decrease in ABA concentration in other cultivars (Mraz, Dukat, Pionir, Trezor) was confirmed.
PB  - Beograd : Društvo genetičara Srbije
PB  - Beograd : Društvo selekcionera i semenara Republike Srbije
C3  - Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11.05.2018.
T1  - Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode
T1  - Accumulation rates of abscisic acid and aldehyde oxidase activity in Pisum sativum L. in response to water deficit
EP  - 38
SP  - 37
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3089
ER  - 

@conference{
author = "Petrović, Gordana and Stikić, Radmila and Živanović, Tomislav and Nikolić, Zorica and Marjanović, Milena and Petrović, Ivana and Samardžić, Jelena",
year = "2018",
abstract = "Izlaganje stresnim uslovima životne sredine, kao što je suša, ima negativan efekat na rast biljaka. Abcisinska kiselina (ABA) se akumulira u uslovima osmotskog stresa i igra važnu ulogu u odgovoru na stres i u toleranciji biljaka. Cilj ovog istraživanja je bio da se odredi koncentracija ABA i ekspresija aldehid oksidaznog enzima (AO) kod 7 sorti stočnog graška u početnim fazama klijanja semena i u stadijumu trećeg para listova. Osmotski stres je stimulisan primenom PEG 6000. Stres suše je izazvan prekidanjem zalivanja. Koncentracija ABA je merena Elisa metodom. U nadzemnom delu ponika utvrđeno je značajno povećanje sadržaja ABA kod jedne grupe sorti (Javor, Partner, Junior) i smanjenje koncentracije ABA kod drugih sorti (Mraz, Dukat, Pionir, Trezor)., Exposure to to environmental stresses, such as drought, causes adverse effects on the growth of plants. Abscisic acid (ABA) accumulates under osmotic stress conditions and plays an important role in the stress response and tolerance of plants. The aim of this research was to determine the rates of ABA and the expression of Aldehyde oxidase enzyme (AO) among 7 cultivars of field pea at early seedling stage and at stage of third pair of leaves. Osmotic stress was stimulated using PEG 6000. The drought stress was induced by a termination of irrigation. ABA concentration was measured by the Elisa method. In the shoot of seedlings a significant increase in ABA content in one group of cultivars (Javor, Partner, Junior), and a decrease in ABA concentration in other cultivars (Mraz, Dukat, Pionir, Trezor) was confirmed.",
publisher = "Beograd : Društvo genetičara Srbije, Beograd : Društvo selekcionera i semenara Republike Srbije",
journal = "Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11.05.2018.",
title = "Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode, Accumulation rates of abscisic acid and aldehyde oxidase activity in Pisum sativum L. in response to water deficit",
pages = "38-37",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3089"
}

Petrović, G., Stikić, R., Živanović, T., Nikolić, Z., Marjanović, M., Petrović, I.,& Samardžić, J.. (2018). Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode. in Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11.05.2018.
Beograd : Društvo genetičara Srbije., 37-38.
https://hdl.handle.net/21.15107/rcub_fiver_3089

Petrović G, Stikić R, Živanović T, Nikolić Z, Marjanović M, Petrović I, Samardžić J. Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode. in Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11.05.2018.. 2018;:37-38.
https://hdl.handle.net/21.15107/rcub_fiver_3089 .

Petrović, Gordana, Stikić, Radmila, Živanović, Tomislav, Nikolić, Zorica, Marjanović, Milena, Petrović, Ivana, Samardžić, Jelena, "Akumulacija abscisinske kiseline i aldehid oksidazna aktivnost kod Pisum sativum L. u uslovima nedostatka vode" in Zbornik apstrakata, 6. Simpozijum Sekcije za oplemenjivanje organizama Društva genetičara Srbije i 9. Simpozijum Društva selekcionera i semenara Republike Srbije, Vrnjačka Banja, 7-11.05.2018. (2018):37-38,
https://hdl.handle.net/21.15107/rcub_fiver_3089 .