TY  - CONF
AU  - Stanković, Ivana
AU  - Zečević, Katarina
AU  - Ćurčić, Živko
AU  - Krstić, Branka
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4173
AB  - Šećerna repa (Beta vulgaris var. saccharifera L.) je gajena biljka iz porodice pepeljuga sa visokom koncentracijom saharoze u korenu i predstavlja najvažniju industrijsku biljku za dobijanje šećera u umerenom klimatu. U Srbiji, šećerna repa takođe predstavlja vrlo profitabilnu kulturu i prema podacima iz 2022. godine gaji se na 34728 ha. Jedno od najznačajnijih oboljenja šećerne repe u Evropi je virusno žutilo koje prouzrokuju u pojedinačnoj ili mešanoj zarazi četiri virusa: virus zapadne žutice šećerne repe (beet western yellows virus, BWYV), virus blagog žutila šećerne repe (beet mild yellowing virus, BMYV), virus hloroze šećerne repe (beet chlorosis virus, BChV) i virus žutice šećerne repe (beet yellows virus, BYV).
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.
T1  - Molekularna detekcija i identifikacija virusa žutice šećerne repe prouzrokovača virusnog žutila šećerne repe u Srbiji
EP  - 35
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4173
ER  - 

@conference{
author = "Stanković, Ivana and Zečević, Katarina and Ćurčić, Živko and Krstić, Branka",
year = "2023",
abstract = "Šećerna repa (Beta vulgaris var. saccharifera L.) je gajena biljka iz porodice pepeljuga sa visokom koncentracijom saharoze u korenu i predstavlja najvažniju industrijsku biljku za dobijanje šećera u umerenom klimatu. U Srbiji, šećerna repa takođe predstavlja vrlo profitabilnu kulturu i prema podacima iz 2022. godine gaji se na 34728 ha. Jedno od najznačajnijih oboljenja šećerne repe u Evropi je virusno žutilo koje prouzrokuju u pojedinačnoj ili mešanoj zarazi četiri virusa: virus zapadne žutice šećerne repe (beet western yellows virus, BWYV), virus blagog žutila šećerne repe (beet mild yellowing virus, BMYV), virus hloroze šećerne repe (beet chlorosis virus, BChV) i virus žutice šećerne repe (beet yellows virus, BYV).",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.",
title = "Molekularna detekcija i identifikacija virusa žutice šećerne repe prouzrokovača virusnog žutila šećerne repe u Srbiji",
pages = "35-35",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4173"
}

Stanković, I., Zečević, K., Ćurčić, Ž.,& Krstić, B.. (2023). Molekularna detekcija i identifikacija virusa žutice šećerne repe prouzrokovača virusnog žutila šećerne repe u Srbiji. in Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.
Beograd : Društvo za zaštitu bilja Srbije., 35-35.
https://hdl.handle.net/21.15107/rcub_fiver_4173

Stanković I, Zečević K, Ćurčić Ž, Krstić B. Molekularna detekcija i identifikacija virusa žutice šećerne repe prouzrokovača virusnog žutila šećerne repe u Srbiji. in Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023.. 2023;:35-35.
https://hdl.handle.net/21.15107/rcub_fiver_4173 .

Stanković, Ivana, Zečević, Katarina, Ćurčić, Živko, Krstić, Branka, "Molekularna detekcija i identifikacija virusa žutice šećerne repe prouzrokovača virusnog žutila šećerne repe u Srbiji" in Zbornik rezimea radova, 17. Simpozijum o zaštiti bilja, Zlatibor, 27-30. novembar 2023. (2023):35-35,
https://hdl.handle.net/21.15107/rcub_fiver_4173 .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Stanković, Ivana
AU  - Nikolić, Zorica
AU  - Vasiljević, Sanja
AU  - Tamindžić, Gordana
AU  - Krstić, Branka
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2511
AB  - U usevu pasulja i boranije širom sveta zabeleženo je preko 200 oboljenja različite
etiologije, međutim u mnogim delovima sveta najznačajnije štete u proizvodnji
mahunarki izazivaju upravo biljni virusi. Pasulj je domaćin 44 različita virusa
među kojima su najvažniji virus običnog mozika pasulja (Bean common mosaic
virus, BCMV), virus običnog nekrotičnog mozaika pasulja (Bean common mosaic
necrosis virus, BCMNV), virus žutog mozaika pasulja (Bean yellow mosaic virus,
BYMV) i virus mozaika krastavca (Cucumber mosaic virus, CMV). Virusi pasulja
se prenose semenom i/ili vašima na neperzistentan način, što je jedan od glavnih
uzroka široke rasprostranjenosti ovih virusa u svetu. Virusna oboljenja na pasulju i
boraniji se ispoljavaju u vidu različitih tipova simptoma, a najčešći su mozaik, šarenilo,
deformacija lišća i promene na mahunama, koji imaju za posledicu smanjenje
porasta biljaka i prinosa.
AB  - Over 200 diseases with different etiologies have been determined in dry beans
and green beans. However, viral diseases seem to cause the greatest economic damage
to legume production in most parts of the world. Dry beans can be infected by
44 different viruses including Bean common mosaic virus (BCMV), Bean common
mosaic necrosis virus (BCMNV), Bean yellow mosaic virus (BYMV) and Cucumber
mosaic virus (CMV). Transmission through seeds and/or spread by aphids in
non-persistent manner play important role in worldwide distribution of bean viruses.
Viral diseases on dry and green beans can have an array of symptoms. The most
common symptoms are the mosaic pattern, variegation or deformation of leaves
and pods, resulting in reduced plant growth and yield.
PB  - Novi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultet
T2  - Biljni lekar
T1  - Najznačajnije viroze pasulja i boranije
T1  - The most important viruses of beans
EP  - 786
IS  - 6
SP  - 773
VL  - 49
DO  - 10.5937/BiljLek2106773M
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Stanković, Ivana and Nikolić, Zorica and Vasiljević, Sanja and Tamindžić, Gordana and Krstić, Branka",
year = "2021",
abstract = "U usevu pasulja i boranije širom sveta zabeleženo je preko 200 oboljenja različite
etiologije, međutim u mnogim delovima sveta najznačajnije štete u proizvodnji
mahunarki izazivaju upravo biljni virusi. Pasulj je domaćin 44 različita virusa
među kojima su najvažniji virus običnog mozika pasulja (Bean common mosaic
virus, BCMV), virus običnog nekrotičnog mozaika pasulja (Bean common mosaic
necrosis virus, BCMNV), virus žutog mozaika pasulja (Bean yellow mosaic virus,
BYMV) i virus mozaika krastavca (Cucumber mosaic virus, CMV). Virusi pasulja
se prenose semenom i/ili vašima na neperzistentan način, što je jedan od glavnih
uzroka široke rasprostranjenosti ovih virusa u svetu. Virusna oboljenja na pasulju i
boraniji se ispoljavaju u vidu različitih tipova simptoma, a najčešći su mozaik, šarenilo,
deformacija lišća i promene na mahunama, koji imaju za posledicu smanjenje
porasta biljaka i prinosa., Over 200 diseases with different etiologies have been determined in dry beans
and green beans. However, viral diseases seem to cause the greatest economic damage
to legume production in most parts of the world. Dry beans can be infected by
44 different viruses including Bean common mosaic virus (BCMV), Bean common
mosaic necrosis virus (BCMNV), Bean yellow mosaic virus (BYMV) and Cucumber
mosaic virus (CMV). Transmission through seeds and/or spread by aphids in
non-persistent manner play important role in worldwide distribution of bean viruses.
Viral diseases on dry and green beans can have an array of symptoms. The most
common symptoms are the mosaic pattern, variegation or deformation of leaves
and pods, resulting in reduced plant growth and yield.",
publisher = "Novi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultet",
journal = "Biljni lekar",
title = "Najznačajnije viroze pasulja i boranije, The most important viruses of beans",
pages = "786-773",
number = "6",
volume = "49",
doi = "10.5937/BiljLek2106773M"
}

Milošević, D., Ignjatov, M., Stanković, I., Nikolić, Z., Vasiljević, S., Tamindžić, G.,& Krstić, B.. (2021). Najznačajnije viroze pasulja i boranije. in Biljni lekar
Novi Sad : Univerzitet u Novom Sadu, Poljoprivredni fakultet., 49(6), 773-786.
https://doi.org/10.5937/BiljLek2106773M

Milošević D, Ignjatov M, Stanković I, Nikolić Z, Vasiljević S, Tamindžić G, Krstić B. Najznačajnije viroze pasulja i boranije. in Biljni lekar. 2021;49(6):773-786.
doi:10.5937/BiljLek2106773M .

Milošević, Dragana, Ignjatov, Maja, Stanković, Ivana, Nikolić, Zorica, Vasiljević, Sanja, Tamindžić, Gordana, Krstić, Branka, "Najznačajnije viroze pasulja i boranije" in Biljni lekar, 49, no. 6 (2021):773-786,
https://doi.org/10.5937/BiljLek2106773M . .

TY  - CONF
AU  - Krstić, Branka
AU  - Paunović, Svetlana
AU  - Jasnić, Stevan
AU  - Bagi, Ferenc
AU  - Mijatović, Mirjana
AU  - Starović, Mira
AU  - Kuzmanović, Slobodan
AU  - Delibašić, Goran
AU  - Milošević, Drago
AU  - Jevremović, Darko
AU  - Milošević, Dragana
AU  - Stanković, Ivana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2429
AB  - Otkriće biljnih virusa vezuje se za 1898. godinu kada je Martinus Willem Beijerinck proučavajući mozak duvana ukazao na novi svet infektivnih agenasa („contagium vivum fluidum“). U Srbiji tridesetih godina prošlog veka, proučavanja biljnih virusa započela su naučnim radom Mladena Josifovića na šarki šljive. Posle Drugog svetskog rata, Dragoljub Šutić, Veljko Nikolić i Mitar Jordović uvode naučne eksperimentalne metode u istraživanja viroza, i time postavljaju temelje razvoja proučavanja biljnih virusa kod nas. Razvoj virusologije u Srbiji, kao i u većini zemalja, obuhvatao je nekoliko faza: početnu deskriptivnu (otkrivanje i opisivanje brojnih viroza), potom epidemiološku (proučavanje načina održavanja i širenja virusa), fizičko-hemijsku (proučavanje morfoloških osobina i hemijskog sastava virusa), molekularno-biološku fazu (razumevanje strukture i funkcije genoma virusa, mehanizama replikacije, unapređenje starih i uvođenje novih metoda detekcije i identifikacije virusa i njihovih sojeva, pravilna taksonomija virusa u skladu sa poreklom i evolutivnim međuodnosima).
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.
T1  - Istorijski razvoj biljne virusologije u Srbiji
EP  - 20
SP  - 19
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2429
ER  - 

@conference{
author = "Krstić, Branka and Paunović, Svetlana and Jasnić, Stevan and Bagi, Ferenc and Mijatović, Mirjana and Starović, Mira and Kuzmanović, Slobodan and Delibašić, Goran and Milošević, Drago and Jevremović, Darko and Milošević, Dragana and Stanković, Ivana",
year = "2021",
abstract = "Otkriće biljnih virusa vezuje se za 1898. godinu kada je Martinus Willem Beijerinck proučavajući mozak duvana ukazao na novi svet infektivnih agenasa („contagium vivum fluidum“). U Srbiji tridesetih godina prošlog veka, proučavanja biljnih virusa započela su naučnim radom Mladena Josifovića na šarki šljive. Posle Drugog svetskog rata, Dragoljub Šutić, Veljko Nikolić i Mitar Jordović uvode naučne eksperimentalne metode u istraživanja viroza, i time postavljaju temelje razvoja proučavanja biljnih virusa kod nas. Razvoj virusologije u Srbiji, kao i u većini zemalja, obuhvatao je nekoliko faza: početnu deskriptivnu (otkrivanje i opisivanje brojnih viroza), potom epidemiološku (proučavanje načina održavanja i širenja virusa), fizičko-hemijsku (proučavanje morfoloških osobina i hemijskog sastava virusa), molekularno-biološku fazu (razumevanje strukture i funkcije genoma virusa, mehanizama replikacije, unapređenje starih i uvođenje novih metoda detekcije i identifikacije virusa i njihovih sojeva, pravilna taksonomija virusa u skladu sa poreklom i evolutivnim međuodnosima).",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.",
title = "Istorijski razvoj biljne virusologije u Srbiji",
pages = "20-19",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2429"
}

Krstić, B., Paunović, S., Jasnić, S., Bagi, F., Mijatović, M., Starović, M., Kuzmanović, S., Delibašić, G., Milošević, D., Jevremović, D., Milošević, D.,& Stanković, I.. (2021). Istorijski razvoj biljne virusologije u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.
Beograd : Društvo za zaštitu bilja Srbije., 19-20.
https://hdl.handle.net/21.15107/rcub_fiver_2429

Krstić B, Paunović S, Jasnić S, Bagi F, Mijatović M, Starović M, Kuzmanović S, Delibašić G, Milošević D, Jevremović D, Milošević D, Stanković I. Istorijski razvoj biljne virusologije u Srbiji. in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021.. 2021;:19-20.
https://hdl.handle.net/21.15107/rcub_fiver_2429 .

Krstić, Branka, Paunović, Svetlana, Jasnić, Stevan, Bagi, Ferenc, Mijatović, Mirjana, Starović, Mira, Kuzmanović, Slobodan, Delibašić, Goran, Milošević, Drago, Jevremović, Darko, Milošević, Dragana, Stanković, Ivana, "Istorijski razvoj biljne virusologije u Srbiji" in Zbornik rezimea radova, 16. Savetovanje o zaštiti bilja, Zlatibor, 22-25. februar 2021. (2021):19-20,
https://hdl.handle.net/21.15107/rcub_fiver_2429 .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Vlajić, Slobodan
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2044
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Turnip Yellows Virus on Cabbage in Serbia
EP  - 998
IS  - 3
SP  - 997
VL  - 104
DO  - 10.1094/PDIS-08-19-1682-PDN
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Vlajić, Slobodan and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Stanković, Ivana and Krstić, Branka",
year = "2020",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Turnip Yellows Virus on Cabbage in Serbia",
pages = "998-997",
number = "3",
volume = "104",
doi = "10.1094/PDIS-08-19-1682-PDN"
}

Milošević, D., Ignjatov, M., Vlajić, S., Nikolić, Z., Gvozdanović-Varga, J., Stanković, I.,& Krstić, B.. (2020). First Report of Turnip Yellows Virus on Cabbage in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 104(3), 997-998.
https://doi.org/10.1094/PDIS-08-19-1682-PDN

Milošević D, Ignjatov M, Vlajić S, Nikolić Z, Gvozdanović-Varga J, Stanković I, Krstić B. First Report of Turnip Yellows Virus on Cabbage in Serbia. in Plant Disease. 2020;104(3):997-998.
doi:10.1094/PDIS-08-19-1682-PDN .

Milošević, Dragana, Ignjatov, Maja, Vlajić, Slobodan, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Stanković, Ivana, Krstić, Branka, "First Report of Turnip Yellows Virus on Cabbage in Serbia" in Plant Disease, 104, no. 3 (2020):997-998,
https://doi.org/10.1094/PDIS-08-19-1682-PDN . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Marjanović-Jeromela, Ana
AU  - Stanković, Ivana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Krstić, Branka
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1896
AB  - In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank.
AB  - Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia
T1  - Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji
EP  - 87
IS  - 3
SP  - 82
VL  - 56
DO  - 10.5937/ratpov56-23514
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Marjanović-Jeromela, Ana and Stanković, Ivana and Nikolić, Zorica and Tamindžić, Gordana and Krstić, Branka",
year = "2019",
abstract = "In 2018, a total of 58 mustard samples from two different localities in Serbia (Rimski Šančevi and Senta) were collected and tested for the presence of Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) by DAS-ELTSA. TuYV was serologically detected in 42 tested samples while all collected samples were negative for CaMV and TuMV. By using aphid transmission tests, five test plants were inoculated with two ELTSApositive TuYV samples of naturally infected mustard plants. Virus species identification was performed by amplification of a 780 bp fragment in all tested samples using the specific primers TuYVorf0F/TuYVorf0R. The RTPCR products from two isolates 88Sal (MK144816) and 98Bni (MK144817) were sequenced and compared with the GenBank sequences of TuYV. Serbian isolates showed the highest identity with Poland TuYV isolate (EU022489). Phylogenetic analysis showed that TuYV isolates from Serbia were clustered with other TuYV sequences retrieved from the GenBank., Tokom 2018. godine, sa dva lokaliteta gajenja slačice, ukupno je sakupljeno 58 uzoraka koji su serološki testirani na prisustvo Turnip yellow virus (TuYV), Cauliflower mosaic virus (CaMV) i Turnip mosaic virus (TuMV) korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV serološki je dokazano u 42 sakupljena uzorka slačice, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrana su dva izolata TuYV prirodno zaraženih biljaka slačice, koji su uspešno preneti vašima na pet različitih test biljaka, čime je potvrđena infektivna priroda oboljenja. Molekularna detekcija obavljena je amplifikacijom fragmenta dužine 780 bp kod četiri ispitivana izolata korišćenjem specifičnih prajmera TuYVorf0F i TuYVorfOR. RT-PCR produkti izolata 88Sal i 98Bni su sekvencirani (MK144816 i MK144817) i upoređeni sa TuYV sekvencama dostupnim u GenBank bazi podataka. Izolati iz Srbije su pokazali najviši stepen nukleotidne sličnosti od 98,9% i 99% (100% aminokiselinska sličnost) sa izolatom TuYV Br iz Poljske (EU022489). Filogenetska analiza pokazala je grupisanje TuYV izolata iz Srbije zajedno sa ostalim TuYV izolatima iz GenBank baze podataka.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia, Molekularna karakterizacija Turnip yellows virus - novog patogena slačice u Srbiji",
pages = "87-82",
number = "3",
volume = "56",
doi = "10.5937/ratpov56-23514"
}

Milošević, D., Ignjatov, M., Marjanović-Jeromela, A., Stanković, I., Nikolić, Z., Tamindžić, G.,& Krstić, B.. (2019). Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 56(3), 82-87.
https://doi.org/10.5937/ratpov56-23514

Milošević D, Ignjatov M, Marjanović-Jeromela A, Stanković I, Nikolić Z, Tamindžić G, Krstić B. Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2019;56(3):82-87.
doi:10.5937/ratpov56-23514 .

Milošević, Dragana, Ignjatov, Maja, Marjanović-Jeromela, Ana, Stanković, Ivana, Nikolić, Zorica, Tamindžić, Gordana, Krstić, Branka, "Molecular characterization of Turnip yellows virus: A new pathogen of mustards in Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 56, no. 3 (2019):82-87,
https://doi.org/10.5937/ratpov56-23514 . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Stanković, Ivana
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Krstić, Branka
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1765
AB  - A two-year investigation (2009-2010) of the presence and distribution of pepper (Capsicum annuum L.) viruses in Serbia revealed that viruses occur each year in open-field production. Disease incidence, as estimated by the number of symptomatic plants in the field, highly varied depending on the year and sampling locality. Disease incidence ranged from 20% to 60%. Four viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), of which PVY was predominant, were detected by serological testing of pepper samples collected from many localities in Serbia. Molecular detection of PVY was performed based on amplification of a 975 bp fragment in all tested samples, using the specific primers PVYc/PVYd that amplify the gene for P1 protein. The RT-PCR products derived from the four isolates (PL-28-09, PL-15-09, PL-3-10, PL-108-10) of PVY were sequenced (KC288142, KC288143, KC288144, and KC288144, respectively) and compared with the PVY sequences available in GenBank. Sequence analysis, conducted with MEGA5 software, revealed 99.8-100% nt identity among the four Serbian PVY isolates from pepper. The sequences of PVY isolates from Serbia share the highest nucleotide and amino acid identity with isolates from Slovenia, Croatia, Germany, and tobbaco isolate from Serbia. All of the four Serbian isolates were clustered in sub-group N-1 with other European isolates of necrotic strains.
AB  - Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Intenzitet zaraze bio je različit i kretao se od 20% do 60%, u zavisnosti od ispitivane godine i lokaliteta gajenja paprike. Serološkim testiranjem uzoraka paprike prikupljenih u više lokaliteta u Srbiji, tokom 2009 i 2010. godine detektovani su Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), pri čemu je PVY bio dominantan. Specifičnim prajmerima PVYc/PVYd uz upotrebu RT-PCR metode umnožen je deo genoma od oko 975 bp koji kodira P1 protein. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank bazu podataka, gde su im dodeljeni pristupni brojevi PL-28-09 (KC288142), PL-15-09 (KC288143), PL-3-10 (KC288144), PL-108-10 (KC288144). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,8-100%. Ispitivane sekvence PVY izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Slovenije, Hrvatske, Nemačke i izolatom duvana iz Srbije. Svi ispitivani izolati sa paprike poreklom iz Srbije grupisani su u subklaster nekrotičnih izolata poreklom iz Evrope.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - Occurrence and diversity of viruses infecting pepper in Serbia
T1  - Prisustvo i rasprostranjenost virusa paprike u Srbiji
EP  - 155
IS  - 46
SP  - 141
VL  - 23
DO  - 10.5937/AASer1846141M
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Stanković, Ivana and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Krstić, Branka",
year = "2018",
abstract = "A two-year investigation (2009-2010) of the presence and distribution of pepper (Capsicum annuum L.) viruses in Serbia revealed that viruses occur each year in open-field production. Disease incidence, as estimated by the number of symptomatic plants in the field, highly varied depending on the year and sampling locality. Disease incidence ranged from 20% to 60%. Four viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), of which PVY was predominant, were detected by serological testing of pepper samples collected from many localities in Serbia. Molecular detection of PVY was performed based on amplification of a 975 bp fragment in all tested samples, using the specific primers PVYc/PVYd that amplify the gene for P1 protein. The RT-PCR products derived from the four isolates (PL-28-09, PL-15-09, PL-3-10, PL-108-10) of PVY were sequenced (KC288142, KC288143, KC288144, and KC288144, respectively) and compared with the PVY sequences available in GenBank. Sequence analysis, conducted with MEGA5 software, revealed 99.8-100% nt identity among the four Serbian PVY isolates from pepper. The sequences of PVY isolates from Serbia share the highest nucleotide and amino acid identity with isolates from Slovenia, Croatia, Germany, and tobbaco isolate from Serbia. All of the four Serbian isolates were clustered in sub-group N-1 with other European isolates of necrotic strains., Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Intenzitet zaraze bio je različit i kretao se od 20% do 60%, u zavisnosti od ispitivane godine i lokaliteta gajenja paprike. Serološkim testiranjem uzoraka paprike prikupljenih u više lokaliteta u Srbiji, tokom 2009 i 2010. godine detektovani su Cucumber mosaic virus (CMV), Potato virus Y (PVY), Alfalfa mosaic virus (AMV) and Pepper mild mottle virus (PMMoV), pri čemu je PVY bio dominantan. Specifičnim prajmerima PVYc/PVYd uz upotrebu RT-PCR metode umnožen je deo genoma od oko 975 bp koji kodira P1 protein. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank bazu podataka, gde su im dodeljeni pristupni brojevi PL-28-09 (KC288142), PL-15-09 (KC288143), PL-3-10 (KC288144), PL-108-10 (KC288144). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,8-100%. Ispitivane sekvence PVY izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Slovenije, Hrvatske, Nemačke i izolatom duvana iz Srbije. Svi ispitivani izolati sa paprike poreklom iz Srbije grupisani su u subklaster nekrotičnih izolata poreklom iz Evrope.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "Occurrence and diversity of viruses infecting pepper in Serbia, Prisustvo i rasprostranjenost virusa paprike u Srbiji",
pages = "155-141",
number = "46",
volume = "23",
doi = "10.5937/AASer1846141M"
}

Milošević, D., Ignjatov, M., Stanković, I., Nikolić, Z., Gvozdanović-Varga, J.,& Krstić, B.. (2018). Occurrence and diversity of viruses infecting pepper in Serbia. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 23(46), 141-155.
https://doi.org/10.5937/AASer1846141M

Milošević D, Ignjatov M, Stanković I, Nikolić Z, Gvozdanović-Varga J, Krstić B. Occurrence and diversity of viruses infecting pepper in Serbia. in Acta agriculturae Serbica. 2018;23(46):141-155.
doi:10.5937/AASer1846141M .

Milošević, Dragana, Ignjatov, Maja, Stanković, Ivana, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Krstić, Branka, "Occurrence and diversity of viruses infecting pepper in Serbia" in Acta agriculturae Serbica, 23, no. 46 (2018):141-155,
https://doi.org/10.5937/AASer1846141M . .

TY  - JOUR
AU  - Krstić, Branka
AU  - Stanković, Ivana
AU  - Milošević, Dragana
AU  - Vučurović, Ana
AU  - Zečević, Katarina
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1626
AB  - The number of virus species infecting pepper crops and their incidences has increased considerably over the past 30 years. This is probably due to a combination of factors, including the expansion and intensification of pepper cultivation, the increased global trade of plant material carrying viruses and vectors to new locations, and climate change expanding the geographic range suitable for the viruses and vectors. About 68 viruses are known to infect peppers worldwide. Most pepper viruses are distributed worldwide with the exception of viruses that have been reported only in certain geographic areas. Virus-infected peppers generally cxhibit a variety of symptoms, the most common of which are mosaic, mottle, necrosis and leaf distortion with consequent reductions in crop growth and yield. More than half of known pepper viruses are transmitted by aphids, and the other common pepper viruses are transmitted by whiteflies, thrips, or by contact and/or through the soil. In Serbia, the presence of five viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Tomato spotted wilt orthotospovirus (TSWV), Alfalfa mosaic virus (AMV) and Tobacco mosaic virus (TMV), with the prevalence of CMV and PVY. Some of these viruses cause considerable yield losses. For each economically important virus in Serbia, control measures are reported here.
AB  - Brojnost virusa koji zaražavaju papriku, kao i učestalost njihove pojave, značajno se povećala poslednjih 30 godina. Uzrok tome je, verovatno, kombinacija različitih faktora, kao što su ekspanzija i intenzivnije gajenje paprike, povećan obim međunarodne trgovine biljnog materijala čime je povećan rizik unošenja virusa i njihovih vektora u nove regione sveta i lokalitete, kao i klimatske promene koje odgovaraju širenju virusa i njihovih vektora. Do sada je utvrđeno da 68 virusa može da zarazi papriku. Većina virusa paprike je raširena celim svetom, ali ima i izuzetaka, koji su prisutni samo u određenim delovima sveta. Virusi paprike izazivaju ispoljavanje različitih tipova simptoma, a najčešći su mozaik, šarenilo i deformacija lišća koji imaju za posledicu smanjenje porasta biljaka i prinosa. Više od polovine virusa paprike se prenosi biljnim vašima, a drugi česti virusi se prenose leptirastim vašima, tripsima ili mehanički. U Srbiji je utvrđeno prisustvo pet virusa: virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus bronzavosti paradajza (Tomato spotted wilt orthotospovirus, TSWV), virus mozaika lucerke (Alfalfa mosaic virus, AMV) i virus mozaika duvana (Tobacco mosaic virus, TMV), s tim što su prevalentni CMV i PVY. Neki od tih virusa prouzrokuju značajne gubitke u prinosu. Za svaki ekonomski važan virus u Srbiji navedene su mere kontrole.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Economically important virus disease of pepper in Serbia
T1  - Ekonomski značajne viroze paprike u Srbiji
EP  - 627
IS  - 6
SP  - 610
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1626
ER  - 

@article{
author = "Krstić, Branka and Stanković, Ivana and Milošević, Dragana and Vučurović, Ana and Zečević, Katarina",
year = "2017",
abstract = "The number of virus species infecting pepper crops and their incidences has increased considerably over the past 30 years. This is probably due to a combination of factors, including the expansion and intensification of pepper cultivation, the increased global trade of plant material carrying viruses and vectors to new locations, and climate change expanding the geographic range suitable for the viruses and vectors. About 68 viruses are known to infect peppers worldwide. Most pepper viruses are distributed worldwide with the exception of viruses that have been reported only in certain geographic areas. Virus-infected peppers generally cxhibit a variety of symptoms, the most common of which are mosaic, mottle, necrosis and leaf distortion with consequent reductions in crop growth and yield. More than half of known pepper viruses are transmitted by aphids, and the other common pepper viruses are transmitted by whiteflies, thrips, or by contact and/or through the soil. In Serbia, the presence of five viruses: Cucumber mosaic virus (CMV), Potato virus Y (PVY), Tomato spotted wilt orthotospovirus (TSWV), Alfalfa mosaic virus (AMV) and Tobacco mosaic virus (TMV), with the prevalence of CMV and PVY. Some of these viruses cause considerable yield losses. For each economically important virus in Serbia, control measures are reported here., Brojnost virusa koji zaražavaju papriku, kao i učestalost njihove pojave, značajno se povećala poslednjih 30 godina. Uzrok tome je, verovatno, kombinacija različitih faktora, kao što su ekspanzija i intenzivnije gajenje paprike, povećan obim međunarodne trgovine biljnog materijala čime je povećan rizik unošenja virusa i njihovih vektora u nove regione sveta i lokalitete, kao i klimatske promene koje odgovaraju širenju virusa i njihovih vektora. Do sada je utvrđeno da 68 virusa može da zarazi papriku. Većina virusa paprike je raširena celim svetom, ali ima i izuzetaka, koji su prisutni samo u određenim delovima sveta. Virusi paprike izazivaju ispoljavanje različitih tipova simptoma, a najčešći su mozaik, šarenilo i deformacija lišća koji imaju za posledicu smanjenje porasta biljaka i prinosa. Više od polovine virusa paprike se prenosi biljnim vašima, a drugi česti virusi se prenose leptirastim vašima, tripsima ili mehanički. U Srbiji je utvrđeno prisustvo pet virusa: virus mozaika krastavca (Cucumber mosaic virus, CMV), virus crtičastog mozaika krompira (Potato virus Y, PVY), virus bronzavosti paradajza (Tomato spotted wilt orthotospovirus, TSWV), virus mozaika lucerke (Alfalfa mosaic virus, AMV) i virus mozaika duvana (Tobacco mosaic virus, TMV), s tim što su prevalentni CMV i PVY. Neki od tih virusa prouzrokuju značajne gubitke u prinosu. Za svaki ekonomski važan virus u Srbiji navedene su mere kontrole.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Economically important virus disease of pepper in Serbia, Ekonomski značajne viroze paprike u Srbiji",
pages = "627-610",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1626"
}

Krstić, B., Stanković, I., Milošević, D., Vučurović, A.,& Zečević, K.. (2017). Economically important virus disease of pepper in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 610-627.
https://hdl.handle.net/21.15107/rcub_fiver_1626

Krstić B, Stanković I, Milošević D, Vučurović A, Zečević K. Economically important virus disease of pepper in Serbia. in Biljni lekar. 2017;45(6):610-627.
https://hdl.handle.net/21.15107/rcub_fiver_1626 .

Krstić, Branka, Stanković, Ivana, Milošević, Dragana, Vučurović, Ana, Zečević, Katarina, "Economically important virus disease of pepper in Serbia" in Biljni lekar, 45, no. 6 (2017):610-627,
https://hdl.handle.net/21.15107/rcub_fiver_1626 .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Stanković, Ivana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Krstić, Branka
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1621
AB  - A two-year investigation (2009-2010) of the presence and distribution of pepper viruses in Serbia revealed that viruses occur each year in open-field pepper production. Serological analyses of collected pepper samples using DAS-ELISA test detected the presence of four viruses: Potato virus Y(PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), and Pepper mild mottle virus (PMMoV), which occurred in single or mixed infections. In 2009 the most frequent was PVY (51.21%), while in 2010 CMV was prevalent (50%). Survey of pepper crops in different growing regions indicated the occurrence of a number of symptoms on leaves, stem, flowers and fruit, which resembled those of virus infection, but it was not possible to determine their association with detected virus. Molecular detection of CMV was performed by RT-PCR using specific primers CMV Au1u/Au2d that flank the AMV coat protein gene, as well as part of 5' and 3' non-coding region of subgenome RNA4. Amplified fragments were sequenced, deposited in the GenBank, and assigned by accession numbers, KC288146 (PL-25-09), KC288147 (PL-43-09) and KC288148 (PL-52-09). Sequence analysis, conducted with MEGA5 software, revealed 99,2-99,5% nt identity between the three Serbian CMV isolates from pepper. The sequences of CMV isolates from Serbia share the highest nucleotide and amino acid identity with isolates from America, Australia, Spain and Serbia.
AB  - Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Serološkim analizama sakupljenih uzoraka paprike primenom DAS-ELISA testa dokazano je prisustvo četiri virusa: Potato virus Y (PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) i Pepper mild mottle virus (PMMoV), i to kako u pojedinačnim tako i u mešanim infekcijama. Tokom 2009. godine najčešće detektovan virus bio je PVY (51,21%), dok je 2010. godine prevalentan virus bio CMV (50%). Pregledom useva paprike u različitim lokalitetima gajenja zabeležena je pojava niza simptoma na lišću, stablu, cvetovima i plodovima, koji su upućivali na virusnu zarazu, ali nije bilo moguće utvrditi njihovu povezanost sa detektovanim virusom. Molekularna detekcija CMV kod tri odabrana izolata obavljena je RT-PCR metodom korišćenjem specifičnih prajmera CMV Au1u/Au2d, koji omogućavaju umnožavanje gena za protein omotača i dela 5' i 3' neprepisujućeg regiona subgenomne RNA 4. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank, gde su im dodeljeni pristupni brojevi, KC288146 (PL-25-09), KC288147 (PL-43-09) i KC288148 (PL-52-09). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu, utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,2-99,5%. Ispitivane sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Amerike, Australije, Španije i Srbije.
PB  - Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad
T2  - Biljni lekar
T1  - Presence and distribution of pepper viruses in Serbia
T1  - Prisustvo i rasprostranjenost virusa paprike u Srbiji
EP  - 656
IS  - 6
SP  - 647
VL  - 45
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1621
ER  - 

@article{
author = "Milošević, Dragana and Stanković, Ivana and Ignjatov, Maja and Nikolić, Zorica and Krstić, Branka",
year = "2017",
abstract = "A two-year investigation (2009-2010) of the presence and distribution of pepper viruses in Serbia revealed that viruses occur each year in open-field pepper production. Serological analyses of collected pepper samples using DAS-ELISA test detected the presence of four viruses: Potato virus Y(PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), and Pepper mild mottle virus (PMMoV), which occurred in single or mixed infections. In 2009 the most frequent was PVY (51.21%), while in 2010 CMV was prevalent (50%). Survey of pepper crops in different growing regions indicated the occurrence of a number of symptoms on leaves, stem, flowers and fruit, which resembled those of virus infection, but it was not possible to determine their association with detected virus. Molecular detection of CMV was performed by RT-PCR using specific primers CMV Au1u/Au2d that flank the AMV coat protein gene, as well as part of 5' and 3' non-coding region of subgenome RNA4. Amplified fragments were sequenced, deposited in the GenBank, and assigned by accession numbers, KC288146 (PL-25-09), KC288147 (PL-43-09) and KC288148 (PL-52-09). Sequence analysis, conducted with MEGA5 software, revealed 99,2-99,5% nt identity between the three Serbian CMV isolates from pepper. The sequences of CMV isolates from Serbia share the highest nucleotide and amino acid identity with isolates from America, Australia, Spain and Serbia., Dvogodišnjim poučavanjima (2009-2010) prisustva i rasprostranjenosti virusa u usevu paprike u Srbiji utvrđeno je da se virusi javljaju svake godine u proizvodnji paprike na otvorenom polju. Serološkim analizama sakupljenih uzoraka paprike primenom DAS-ELISA testa dokazano je prisustvo četiri virusa: Potato virus Y (PVY), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV) i Pepper mild mottle virus (PMMoV), i to kako u pojedinačnim tako i u mešanim infekcijama. Tokom 2009. godine najčešće detektovan virus bio je PVY (51,21%), dok je 2010. godine prevalentan virus bio CMV (50%). Pregledom useva paprike u različitim lokalitetima gajenja zabeležena je pojava niza simptoma na lišću, stablu, cvetovima i plodovima, koji su upućivali na virusnu zarazu, ali nije bilo moguće utvrditi njihovu povezanost sa detektovanim virusom. Molekularna detekcija CMV kod tri odabrana izolata obavljena je RT-PCR metodom korišćenjem specifičnih prajmera CMV Au1u/Au2d, koji omogućavaju umnožavanje gena za protein omotača i dela 5' i 3' neprepisujućeg regiona subgenomne RNA 4. Amplifikovani fragmenti su sekvencirani i prijavljeni u GenBank, gde su im dodeljeni pristupni brojevi, KC288146 (PL-25-09), KC288147 (PL-43-09) i KC288148 (PL-52-09). Proračunom genetičke sličnosti sekvenci izolata dobijenih u ovom radu, utvrđen je visok stepen nukleotidne sličnosti, koji se kretao od 99,2-99,5%. Ispitivane sekvence CMV izolata iz Srbije dele najveću nukleotidnu i aminokiselinsku sličnost sa izolatima iz Amerike, Australije, Španije i Srbije.",
publisher = "Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad",
journal = "Biljni lekar",
title = "Presence and distribution of pepper viruses in Serbia, Prisustvo i rasprostranjenost virusa paprike u Srbiji",
pages = "656-647",
number = "6",
volume = "45",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1621"
}

Milošević, D., Stanković, I., Ignjatov, M., Nikolić, Z.,& Krstić, B.. (2017). Presence and distribution of pepper viruses in Serbia. in Biljni lekar
Univerzitet u Novom Sadu - Poljoprivredni fakultet - Departman za zaštitu bilja i životne sredine "dr Pavla Vukasovića", Novi Sad., 45(6), 647-656.
https://hdl.handle.net/21.15107/rcub_fiver_1621

Milošević D, Stanković I, Ignjatov M, Nikolić Z, Krstić B. Presence and distribution of pepper viruses in Serbia. in Biljni lekar. 2017;45(6):647-656.
https://hdl.handle.net/21.15107/rcub_fiver_1621 .

Milošević, Dragana, Stanković, Ivana, Ignjatov, Maja, Nikolić, Zorica, Krstić, Branka, "Presence and distribution of pepper viruses in Serbia" in Biljni lekar, 45, no. 6 (2017):647-656,
https://hdl.handle.net/21.15107/rcub_fiver_1621 .

TY  - JOUR
AU  - Vučurović, I.
AU  - Vučurović, Ana
AU  - Nikolić, D.
AU  - Bulajić, Aleksandra
AU  - Milošević, Dragana
AU  - Krstić, Branka
AU  - Stanković, Ivana
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1505
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Leek yellow stripe virus in Leek in Serbia
EP  - 231
IS  - 1
SP  - 230
VL  - 100
DO  - 10.1094/PDIS-04-15-0432-PDN
ER  - 

@article{
author = "Vučurović, I. and Vučurović, Ana and Nikolić, D. and Bulajić, Aleksandra and Milošević, Dragana and Krstić, Branka and Stanković, Ivana",
year = "2016",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Leek yellow stripe virus in Leek in Serbia",
pages = "231-230",
number = "1",
volume = "100",
doi = "10.1094/PDIS-04-15-0432-PDN"
}

Vučurović, I., Vučurović, A., Nikolić, D., Bulajić, A., Milošević, D., Krstić, B.,& Stanković, I.. (2016). First Report of Leek yellow stripe virus in Leek in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 100(1), 230-231.
https://doi.org/10.1094/PDIS-04-15-0432-PDN

Vučurović I, Vučurović A, Nikolić D, Bulajić A, Milošević D, Krstić B, Stanković I. First Report of Leek yellow stripe virus in Leek in Serbia. in Plant Disease. 2016;100(1):230-231.
doi:10.1094/PDIS-04-15-0432-PDN .

Vučurović, I., Vučurović, Ana, Nikolić, D., Bulajić, Aleksandra, Milošević, Dragana, Krstić, Branka, Stanković, Ivana, "First Report of Leek yellow stripe virus in Leek in Serbia" in Plant Disease, 100, no. 1 (2016):230-231,
https://doi.org/10.1094/PDIS-04-15-0432-PDN . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Marjanović-Jeromela, Ana
AU  - Krstić, Branka
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1516
AB  - A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates.
AB  - Tokom 2014. godine, sa šest lokaliteta gajenja uljane repice, ukupno je sakupljeno 86 uzorka koji su serološki testirani na prisustvo virusa žutice postrne repe [Turnip yellow virus (TuYV)], virusa mozaika karfiola [Cauliflower mosaic virus (CaMV)] i virusa mozaika postrne repe [Turnip mosaic virus (TuMV)], korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV dokazano je u 60 (69.77%) prikupljenih uzoraka, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka uljane repice poreklom iz različitih lokaliteta koji su uspešno preneti vašima na tri različite test biljke, čime je potvrđena infektivna priroda oboljenja. Prisustvo TuYV u ELISA pozitivním uzorcima je potvrđeno korišćenjem RT-PCR i sekvencioniranjem. Poređenjem dobijenih sekvenci sa sekvencama dostupnih u GenBank bazi podataka, potvrđena je autentičnost serološki detektovanih virusa. Na osnovu sekvenci P0 gena izolata TuYV, utvrđena je pripadnost ispitivanih izolata iz uljane repice grupi sa ostalim TuYV izolatima, koji su jasno razdvojeni od Beet western yellows virus (BWYV) izolata.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia
T1  - Prisustvo virusa žutice postrne repe na uljanoj repici (Brassica napus L.) u Srbiji
EP  - 44
IS  - 1-2
SP  - 37
VL  - 31
DO  - 10.2298/PIF1602037M
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Stanković, Ivana and Bulajić, Aleksandra and Marjanović-Jeromela, Ana and Krstić, Branka",
year = "2016",
abstract = "A total of 86 oilseed rape samples from six crops in different localities were collected during 2014 and analyzed for the presence of Turnip yellows virus (TuYV), Cauliflower mosaic virus (CaMV) and Turnip mosaic virus (TuMV) using commercial double-antibody sandwich (DAS)-ELISA kits. TuYV was serologically detected in 60 collected samples (69.77%), and none of the samples tested were positive for CaMV and TuMV. Six selected TuYV isolates were successfully transmitted by Myzus persicae to three test plants, confirming the infectious nature of the disease. In the selected ELISA-positive samples, the presence of TuYV was further confirmed by RT-PCR and sequencing. A comparison of the obtained sequence with those available in GenBank confirmed the presence of TuYV in oilseed rape samples. An analysis of P0 gene sequence data for a subset of these isolates showed they clustered with the known TuYV and were distinct from Beet western yellows virus (BWYV) isolates., Tokom 2014. godine, sa šest lokaliteta gajenja uljane repice, ukupno je sakupljeno 86 uzorka koji su serološki testirani na prisustvo virusa žutice postrne repe [Turnip yellow virus (TuYV)], virusa mozaika karfiola [Cauliflower mosaic virus (CaMV)] i virusa mozaika postrne repe [Turnip mosaic virus (TuMV)], korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo TuYV dokazano je u 60 (69.77%) prikupljenih uzoraka, dok prisustvo CaMV i TuMV nije dokazano ni u jednom od testiranih uzoraka. Za dalja istraživanja odabrano je šest uzoraka prirodno zaraženih biljaka uljane repice poreklom iz različitih lokaliteta koji su uspešno preneti vašima na tri različite test biljke, čime je potvrđena infektivna priroda oboljenja. Prisustvo TuYV u ELISA pozitivním uzorcima je potvrđeno korišćenjem RT-PCR i sekvencioniranjem. Poređenjem dobijenih sekvenci sa sekvencama dostupnih u GenBank bazi podataka, potvrđena je autentičnost serološki detektovanih virusa. Na osnovu sekvenci P0 gena izolata TuYV, utvrđena je pripadnost ispitivanih izolata iz uljane repice grupi sa ostalim TuYV izolatima, koji su jasno razdvojeni od Beet western yellows virus (BWYV) izolata.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia, Prisustvo virusa žutice postrne repe na uljanoj repici (Brassica napus L.) u Srbiji",
pages = "44-37",
number = "1-2",
volume = "31",
doi = "10.2298/PIF1602037M"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Stanković, I., Bulajić, A., Marjanović-Jeromela, A.,& Krstić, B.. (2016). The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 31(1-2), 37-44.
https://doi.org/10.2298/PIF1602037M

Milošević D, Ignjatov M, Nikolić Z, Stanković I, Bulajić A, Marjanović-Jeromela A, Krstić B. The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia. in Pesticidi i fitomedicina. 2016;31(1-2):37-44.
doi:10.2298/PIF1602037M .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Stanković, Ivana, Bulajić, Aleksandra, Marjanović-Jeromela, Ana, Krstić, Branka, "The presence of turnip yellows virus in oilseed rape (Brassica napus L.) in Serbia" in Pesticidi i fitomedicina, 31, no. 1-2 (2016):37-44,
https://doi.org/10.2298/PIF1602037M . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Petrović, Gordana
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1464
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cucumber mosaic virus Causing Chlorotic Mottle on Pot Marigold (Calendula officinalis) in Serbia
EP  - 736
IS  - 5
SP  - 736
VL  - 99
DO  - 10.1094/PDIS-11-14-1208-PDN
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Petrović, Gordana and Stanković, Ivana and Krstić, Branka",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cucumber mosaic virus Causing Chlorotic Mottle on Pot Marigold (Calendula officinalis) in Serbia",
pages = "736-736",
number = "5",
volume = "99",
doi = "10.1094/PDIS-11-14-1208-PDN"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Gvozdanović-Varga, J., Petrović, G., Stanković, I.,& Krstić, B.. (2015). First Report of Cucumber mosaic virus Causing Chlorotic Mottle on Pot Marigold (Calendula officinalis) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(5), 736-736.
https://doi.org/10.1094/PDIS-11-14-1208-PDN

Milošević D, Ignjatov M, Nikolić Z, Gvozdanović-Varga J, Petrović G, Stanković I, Krstić B. First Report of Cucumber mosaic virus Causing Chlorotic Mottle on Pot Marigold (Calendula officinalis) in Serbia. in Plant Disease. 2015;99(5):736-736.
doi:10.1094/PDIS-11-14-1208-PDN .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Petrović, Gordana, Stanković, Ivana, Krstić, Branka, "First Report of Cucumber mosaic virus Causing Chlorotic Mottle on Pot Marigold (Calendula officinalis) in Serbia" in Plant Disease, 99, no. 5 (2015):736-736,
https://doi.org/10.1094/PDIS-11-14-1208-PDN . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Krstić, Branka
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1407
AB  - During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Detection and molecular characterization of pepper mild mottle virus in Serbia
EP  - 663
IS  - 2
SP  - 651
VL  - 47
DO  - 10.2298/GENSR1502651M
ER  - 

@article{
author = "Milošević, Dragana and Stanković, Ivana and Bulajić, Aleksandra and Ignjatov, Maja and Nikolić, Zorica and Petrović, Gordana and Krstić, Branka",
year = "2015",
abstract = "During 2009 and 2010, a survey was conducted in pepper crops to detect the possible presence of Pepper mild mottle virus (PMMoV) in Serbia. A total of 239 pepper samples from 39 crops at 26 localities were collected and analyzed for the presence of PMMoV, Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Alfalfa mosaic virus (AMV), using DAS-ELISA test. Although it was detected in a small percentage, PMMoV could pose a threat to pepper production in Serbia due to its rapid seed-borne spread. Presence of PMMoV was confirmed by serological and biological detection, followed by conventional reverse transcription RT-PCR, using primers specific for the RNA-dependent RNA polymerase (RdRp) and the coat protein (CP) genes. Molecular identification confirmed that the Serbian isolates belong to PMMoV pathotypes P1,2 which do not break the resistance gene L-3. Reconstructed phylogenetic tree confirmed the allocation of the Serbian isolates together with the majority of PMMoV isolates which belong to pathotypes P-1,P-2. This study represents the first serological and molecular characterization of PMMoV infection of pepper in Serbia, and provides important data on the population structure. The obtained data could have great influence on pepper production in Serbia as well as future pepper resistance breeding in the country.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Detection and molecular characterization of pepper mild mottle virus in Serbia",
pages = "663-651",
number = "2",
volume = "47",
doi = "10.2298/GENSR1502651M"
}

Milošević, D., Stanković, I., Bulajić, A., Ignjatov, M., Nikolić, Z., Petrović, G.,& Krstić, B.. (2015). Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 47(2), 651-663.
https://doi.org/10.2298/GENSR1502651M

Milošević D, Stanković I, Bulajić A, Ignjatov M, Nikolić Z, Petrović G, Krstić B. Detection and molecular characterization of pepper mild mottle virus in Serbia. in Genetika-Belgrade. 2015;47(2):651-663.
doi:10.2298/GENSR1502651M .

Milošević, Dragana, Stanković, Ivana, Bulajić, Aleksandra, Ignjatov, Maja, Nikolić, Zorica, Petrović, Gordana, Krstić, Branka, "Detection and molecular characterization of pepper mild mottle virus in Serbia" in Genetika-Belgrade, 47, no. 2 (2015):651-663,
https://doi.org/10.2298/GENSR1502651M . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Marjanović-Jeromela, Ana
AU  - Ignjatov, Maja
AU  - Jovičić, Dušica
AU  - Stanković, Ivana
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1438
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Turnip yellows virus on Oilseed Rape in Serbia
EP  - 1869
IS  - 12
SP  - 1869
VL  - 99
DO  - 10.1094/PDIS-05-15-0503-PDN
ER  - 

@article{
author = "Milošević, Dragana and Marjanović-Jeromela, Ana and Ignjatov, Maja and Jovičić, Dušica and Stanković, Ivana and Bulajić, Aleksandra and Krstić, Branka",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Turnip yellows virus on Oilseed Rape in Serbia",
pages = "1869-1869",
number = "12",
volume = "99",
doi = "10.1094/PDIS-05-15-0503-PDN"
}

Milošević, D., Marjanović-Jeromela, A., Ignjatov, M., Jovičić, D., Stanković, I., Bulajić, A.,& Krstić, B.. (2015). First Report of Turnip yellows virus on Oilseed Rape in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(12), 1869-1869.
https://doi.org/10.1094/PDIS-05-15-0503-PDN

Milošević D, Marjanović-Jeromela A, Ignjatov M, Jovičić D, Stanković I, Bulajić A, Krstić B. First Report of Turnip yellows virus on Oilseed Rape in Serbia. in Plant Disease. 2015;99(12):1869-1869.
doi:10.1094/PDIS-05-15-0503-PDN .

Milošević, Dragana, Marjanović-Jeromela, Ana, Ignjatov, Maja, Jovičić, Dušica, Stanković, Ivana, Bulajić, Aleksandra, Krstić, Branka, "First Report of Turnip yellows virus on Oilseed Rape in Serbia" in Plant Disease, 99, no. 12 (2015):1869-1869,
https://doi.org/10.1094/PDIS-05-15-0503-PDN . .

TY  - JOUR
AU  - Milošević, Dragana
AU  - Ignjatov, Maja
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Tamindžić, Gordana
AU  - Stanković, Ivana
AU  - Krstić, Branka
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1477
AB  - During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II.
AB  - Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia
T1  - Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji
EP  - 73
IS  - 2
SP  - 67
VL  - 52
DO  - 10.5937/ratpov52-7814
ER  - 

@article{
author = "Milošević, Dragana and Ignjatov, Maja and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Tamindžić, Gordana and Stanković, Ivana and Krstić, Branka",
year = "2015",
abstract = "During 2014 a total of 67 pot marigold samples from five different localities in the Province in Vojvodina were collected and analysed for the presence of Cucumber mosaic virus (CMV) and Impatiens necrotic spot virus (INSV) using commercial double-antibody sandwich (DAS)-ELISA kits. CMV was detected serologically in all inspected localities in 67.16% collected samples. None of the analysed samples was positive for INSV. The virus was successfully mechanically transmitted to test plants including Chenopodium amaranticolor, C. quinoa, Datura stramonium, Nicotiana tabacum 'Samsun' and N. glutinosa, as well as pot marigold seedlings, confirming the infectious nature of the disease. The presence of CMV in pot marigold plants was further verified by RT-PCR and sequencing, using the specific primers CMV CPfwd/CMVCPrev that amplify coat protein (CP) gene. Phylogenetic analysis based on the CP gene sequences showed clustering of the selected isolates into three subgroups, IA, IB and II, and Serbian CMV isolates from pot marigold belong to subgroup II., Tokom 2014. godine, sa pet različitih lokaliteta gajenja nevena u Vojvodini sakupljeno je ukupno 67 uzorka koji su serološki testirani na prisustvo virusa mozaika krastavca (Cucumber mosaic virus, CMV) i virusa nekrotične pegavosti impatiensa (Impatiens necrotic spot virus, INSV), korišćenjem komercijalno dostupnih kitova za DAS-ELISA test. Prisustvo CMV dokazano je na svih pet pregledanih lokaliteta i to u 67,16% prikupljenih uzoraka, dok prisustvo INSV nije dokazano ni u jednom od testiranih uzoraka. Virus je uspešno prenet mehaničkim inokulacijama test biljaka Chenopodium amaranticolor, C. quinoa, Nicotiana tabacum 'Samsun' i N. glutinosa, kao i na sejance nevena, čime je potvrđena infektivna priroda oboljenja. Prisustvo CMV u biljkama nevena potvrđeno je primenom RT-PCR metode i sekvencioniranjem uz korišćenje specifičnih prajmera CMVCPfwd/CMVCPrev koji omogućavaju umnožavanje gena za protein omotača (CP gena). Filogenetska analiza na osnovu sekvence CP gena pokazala je grupisanje izolata u tri podgrupe, IA, IB i II, a izolati CMV iz nevena iz Srbije grupišu se u podgrupu II.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia, Prisustvo virusa mozaika krastavca na nevenu (Calendula officinalis L.) u Srbiji",
pages = "73-67",
number = "2",
volume = "52",
doi = "10.5937/ratpov52-7814"
}

Milošević, D., Ignjatov, M., Nikolić, Z., Gvozdanović-Varga, J., Tamindžić, G., Stanković, I.,& Krstić, B.. (2015). The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 52(2), 67-73.
https://doi.org/10.5937/ratpov52-7814

Milošević D, Ignjatov M, Nikolić Z, Gvozdanović-Varga J, Tamindžić G, Stanković I, Krstić B. The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2015;52(2):67-73.
doi:10.5937/ratpov52-7814 .

Milošević, Dragana, Ignjatov, Maja, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Tamindžić, Gordana, Stanković, Ivana, Krstić, Branka, "The presence of cucumber mosaic virus in pot marigold (Calendula officinalis L.) in Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 52, no. 2 (2015):67-73,
https://doi.org/10.5937/ratpov52-7814 . .

TY  - JOUR
AU  - Milojević, K.
AU  - Stanković, Ivana
AU  - Vučurović, Ana
AU  - Ristić, D.
AU  - Milošević, Dragana
AU  - Bulajić, Aleksandra
AU  - Krstić, Branka
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1256
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia
EP  - 1004
IS  - 7
SP  - 1004
VL  - 97
DO  - 10.1094/PDIS-01-13-0089-PDN
ER  - 

@article{
author = "Milojević, K. and Stanković, Ivana and Vučurović, Ana and Ristić, D. and Milošević, Dragana and Bulajić, Aleksandra and Krstić, Branka",
year = "2013",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia",
pages = "1004-1004",
number = "7",
volume = "97",
doi = "10.1094/PDIS-01-13-0089-PDN"
}

Milojević, K., Stanković, I., Vučurović, A., Ristić, D., Milošević, D., Bulajić, A.,& Krstić, B.. (2013). First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 97(7), 1004-1004.
https://doi.org/10.1094/PDIS-01-13-0089-PDN

Milojević K, Stanković I, Vučurović A, Ristić D, Milošević D, Bulajić A, Krstić B. First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia. in Plant Disease. 2013;97(7):1004-1004.
doi:10.1094/PDIS-01-13-0089-PDN .

Milojević, K., Stanković, Ivana, Vučurović, Ana, Ristić, D., Milošević, Dragana, Bulajić, Aleksandra, Krstić, Branka, "First Report of Cucumber mosaic virus Infecting Peperomia tuisana in Serbia" in Plant Disease, 97, no. 7 (2013):1004-1004,
https://doi.org/10.1094/PDIS-01-13-0089-PDN . .

TY  - JOUR
AU  - Petrović, Dragana
AU  - Bulajić, Aleksandra
AU  - Stanković, Ivana
AU  - Ignjatov, Maja
AU  - Vujaković, Milka
AU  - Krstić, Branka
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/868
AB  - Pepper is a very popular and profitable crop in Serbia and its production is growing rapidly as well as the importance of diseases caused by viruses. Virus infections interfere with development of pepper plants, reducing yield and fruit quality. More than 45 viruses have been isolated from pepper so far, and in our country the following are considered economically very important: Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), Tomato spotted wilt virus (TSWV) and Potato virus Y (PVY). During 2009, an investigation was conducted regarding the presence and distribution of pepper viruses in Serbia, which included a survey of different localities of transplant production, and both greenhouse and open field pepper crops. Collected samples were tested utilizing a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with polyclonal antisera specific for the detection of economically important pepper viruses: PVY, AMV, CMV, TSWV, TMV and Potato virus X (PVX). In the collected samples, presence of the four viruses (PVY, CMV, TSWV and AMV) was confirmed in single and mixed infections. In tested transplant samples, the most frequently detected virus was CMV (18.18%), while PVY was the most frequent in samples collected in greenhouse and open field pepper crops (32.56% and 51.21% respectively). The presence of TSWV was detected only in greenhouse-grown pepper crops, while the presence of PVX and TMV was not detected during this investigation. The obtained results indicated that PVY and CMV were widely distributed and the most frequent viruses in pepper crops in Serbia. Future investigation should include their detailed biological and molecular characterization, as well as the implementation of appropriate control measures in pepper productions.
AB  - Virusne bolesti paprike mogu ugroziti normalan razvoj biljaka i time značajno umanjiti prinos i kvalitet plodova. U svetu je opisano više od 45 virusa infektivnih za papriku, među kojima su ekonomski najznačajniji virus mozaika duvana (Tobacco mosaic virus, TMV), virus mozaika krastavca (Cucumber mosaic virus, CMV), virus mozaika lucerke (Alfalfa mosaic virus, AMV), virus bronzavosti paradajza (Tomato spotted wilt virus, TSWV) i virus crtičastog mozaika krompira (Potato virus Y, PVY). Prisustvo i rasprostranjenost virusnih zaraza paprike u Srbiji ispitivani su tokom 2009. u različitim lokalitetima proizvodnje rasada, kao i useva paprike gajene u zaštićenom prostoru i na otvorenom polju. Sakupljeni uzorci lišća sa simptomima testirani su DAS-ELISA metodom na prisustvo: PVY, AMV, CMV, TSWV, TMV i virusa mozaika krompira (Potato virus X, PVX). Dobijeni rezultati ispitivanja su ukazali na prisustvo četiri virusa: PVY, CMV, TSWV i AMV, koji su identifikovani u pojedinačnim ili mešanim infekcijama. U testiranim uzorcima paprike iz rasada najzastupljeniji je bio CMV (18,18%), dok je u uzorcima prikupljenim iz plastenika i polja najčešći bio PVY (32,56% i 51,21%). Prisustvo TSWV dokazano je samo na biljkama paprike gajenim u zaštićenom prostoru. Prisustvo PVX i TMV nije ustanovljeno ni u jednom ispitivanom uzorku paprike. Utvrđena značajna rasprostranjenost i zastupljenost PVY i CMV u proizvodnji paprike u Srbiji ukazuje na potrebu detaljne karakterizacije ova dva virusa na biološkom i molekularnom nivou, kao i sprovođenje odgovarajućih mera kontrole.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Presence and distribution of pepper viruses in Serbia
T1  - Prisustvo i rasprostranjenost virusa paprike u Srbiji
EP  - 576
IS  - 2
SP  - 567
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_agrospace_2285
ER  - 

@article{
author = "Petrović, Dragana and Bulajić, Aleksandra and Stanković, Ivana and Ignjatov, Maja and Vujaković, Milka and Krstić, Branka",
year = "2010",
abstract = "Pepper is a very popular and profitable crop in Serbia and its production is growing rapidly as well as the importance of diseases caused by viruses. Virus infections interfere with development of pepper plants, reducing yield and fruit quality. More than 45 viruses have been isolated from pepper so far, and in our country the following are considered economically very important: Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV), Alfalfa mosaic virus (AMV), Tomato spotted wilt virus (TSWV) and Potato virus Y (PVY). During 2009, an investigation was conducted regarding the presence and distribution of pepper viruses in Serbia, which included a survey of different localities of transplant production, and both greenhouse and open field pepper crops. Collected samples were tested utilizing a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) with polyclonal antisera specific for the detection of economically important pepper viruses: PVY, AMV, CMV, TSWV, TMV and Potato virus X (PVX). In the collected samples, presence of the four viruses (PVY, CMV, TSWV and AMV) was confirmed in single and mixed infections. In tested transplant samples, the most frequently detected virus was CMV (18.18%), while PVY was the most frequent in samples collected in greenhouse and open field pepper crops (32.56% and 51.21% respectively). The presence of TSWV was detected only in greenhouse-grown pepper crops, while the presence of PVX and TMV was not detected during this investigation. The obtained results indicated that PVY and CMV were widely distributed and the most frequent viruses in pepper crops in Serbia. Future investigation should include their detailed biological and molecular characterization, as well as the implementation of appropriate control measures in pepper productions., Virusne bolesti paprike mogu ugroziti normalan razvoj biljaka i time značajno umanjiti prinos i kvalitet plodova. U svetu je opisano više od 45 virusa infektivnih za papriku, među kojima su ekonomski najznačajniji virus mozaika duvana (Tobacco mosaic virus, TMV), virus mozaika krastavca (Cucumber mosaic virus, CMV), virus mozaika lucerke (Alfalfa mosaic virus, AMV), virus bronzavosti paradajza (Tomato spotted wilt virus, TSWV) i virus crtičastog mozaika krompira (Potato virus Y, PVY). Prisustvo i rasprostranjenost virusnih zaraza paprike u Srbiji ispitivani su tokom 2009. u različitim lokalitetima proizvodnje rasada, kao i useva paprike gajene u zaštićenom prostoru i na otvorenom polju. Sakupljeni uzorci lišća sa simptomima testirani su DAS-ELISA metodom na prisustvo: PVY, AMV, CMV, TSWV, TMV i virusa mozaika krompira (Potato virus X, PVX). Dobijeni rezultati ispitivanja su ukazali na prisustvo četiri virusa: PVY, CMV, TSWV i AMV, koji su identifikovani u pojedinačnim ili mešanim infekcijama. U testiranim uzorcima paprike iz rasada najzastupljeniji je bio CMV (18,18%), dok je u uzorcima prikupljenim iz plastenika i polja najčešći bio PVY (32,56% i 51,21%). Prisustvo TSWV dokazano je samo na biljkama paprike gajenim u zaštićenom prostoru. Prisustvo PVX i TMV nije ustanovljeno ni u jednom ispitivanom uzorku paprike. Utvrđena značajna rasprostranjenost i zastupljenost PVY i CMV u proizvodnji paprike u Srbiji ukazuje na potrebu detaljne karakterizacije ova dva virusa na biološkom i molekularnom nivou, kao i sprovođenje odgovarajućih mera kontrole.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Presence and distribution of pepper viruses in Serbia, Prisustvo i rasprostranjenost virusa paprike u Srbiji",
pages = "576-567",
number = "2",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_agrospace_2285"
}

Petrović, D., Bulajić, A., Stanković, I., Ignjatov, M., Vujaković, M.,& Krstić, B.. (2010). Presence and distribution of pepper viruses in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 47(2), 567-576.
https://hdl.handle.net/21.15107/rcub_agrospace_2285

Petrović D, Bulajić A, Stanković I, Ignjatov M, Vujaković M, Krstić B. Presence and distribution of pepper viruses in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2010;47(2):567-576.
https://hdl.handle.net/21.15107/rcub_agrospace_2285 .

Petrović, Dragana, Bulajić, Aleksandra, Stanković, Ivana, Ignjatov, Maja, Vujaković, Milka, Krstić, Branka, "Presence and distribution of pepper viruses in Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 47, no. 2 (2010):567-576,
https://hdl.handle.net/21.15107/rcub_agrospace_2285 .