TY  - JOUR
AU  - Umićević, Sonja
AU  - Antić, Marina
AU  - Rajković, Dragana
AU  - Sinkovič, Lovro
AU  - Nagl, Nevena
AU  - Goreta Ban, Smiljana
AU  - Meglič, Vladimir
AU  - Todorović, Vida
AU  - Marjanović-Jeromela, Ana
AU  - Pipan, Barbara
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3784
AB  - Leafy kale (Brassica oleracea var. acephala L.) is one of the most
important Brassicaceae species with health-promoting properties and great
diversity. To investigate variability within and among accessions, 12 accessions
(each accession comprised four individuals/genotypes) from the Balkan region
were genotyped at 12 simple sequence repeats (SSRs) loci. The selected SSR
markers originated from the genomes of B. oleracea (eight), B. rapa (two) and
B. napus (two). DNA was extracted from seeds, cotyledons, shoots, and young
leaves, depending on the germination energy of each seed, using magnetic
extraction method. Genetic analysis included eight accessions from Serbia (24
genotypes), two accessions from Montenegro (eight genotypes), and one
accession from Bosnia and Herzegovina and Croatia (with four genotypes each).
Accession-specific polymorphism was found at the Ol12-FO2 and Ol10-F11a
loci, where genotypes were qualitatively distinguished by horizontal agarose gel
electrophoresis (binary detection). Among 12 loci, the average polymorphic
information content (PIC) was 0.71, with the marker Ol13-C12 proving to be the
78 Umićević et al.
most informative (PIC = 0.89). However, the analysis of molecular variability
(AMOVA) showed 11% diversity between accessions, 19% diversity between
genotypes, and 70% diversity within genotypes, explaining the molecular
diversity of native Balkan leafy kale germplasm. According to the genetic
structure, four genetic groups were formed with an average expected
heterozygosity of 0.70 between clusters. In order to exploit the genetic diversity,
it would be advisable to evaluate these accessions at phenotypic level and use
their potential in breeding programmes.
AB  - Raštan (Brassica oleracea var. acephala L.) je jedna od najvažnijih vrsta iz porodice kupusnjača koja povoljno utiče na zdravlje ljudi i odlikuje se velikom raznovrsnošću. Da bi se istražila varijabilnost unutar i između prinova, 12 prinova (svaka prinova se sastojala od četiri individue/genotipa) sa teritorije Balkanskog poluostrva je genotipizovano koristeći 12 SSR markera. Odabrani SSR markeri potiču iz genoma B. oleracea (osam), B. rapa (dva) i B. napus (dva). DNK je izolovana iz sjemena, kotiledona, izdanaka i mladih listova, u zavisnosti od energije klijanja svakog sjemena, metodom magnetne ekstrakcije. Genetičkom analizom obuhvaćeno je osam prinova iz Srbije (24 genotipa), dvije prinove iz Crne Gore (osam genotipova) i jedna prinova iz Bosne i Hercegovine i Hrvatske (sa po četiri genotipa). Polimorfizam specifičan za prinove pronađen je na lokusima Ol12-FO2 i Ol10-F11a, gdje su genotipovi kvalitativno razlikovani horizontalnom elektroforezom u agaroznom gelu (binarna detekcija). Kod ukupno 12 lokusa, prosječan sadržaj polimorfne informacije (PIC) bio je 0,71, pri čemu se kao najinformativniji pokazao marker Ol13-C12 (PIC = 0,89). Međutim, analiza molekularne varijabilnosti (AMOVA) pokazala je 11% raznovrsnosti između prinova, 19% raznovrsnosti između genotipova i 70% raznovrsnosti unutar genotipova, objašnjavajući molekularnu raznovrsnost izvorne germplazme raštana sa teritorije Balkanskog poluostrva. Prema genetičkoj strukturi formirane su četiri genetičke grupe sa prosječnom očekivanom heterozigotnošću od 0,70 između klastera. Da bi se iskoristio genetički diverzitet, bilo bi preporučljivo da se ove prinove karakterišu 90 Umićević et al. na fenotipskom nivou i da se njihov potencijal iskoristi u programima oplemenjivanja.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
T2  - Agroznanje / Agro-knowledge Journal
T1  - Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.)
T1  - Genetički diverzitet genetičkih resursa raštana (Brassica oleracea var. acephala L.)
EP  - 90
IS  - 3
SP  - 77
VL  - 24
DO  - 10.7251/AGREN2303077U
ER  - 

@article{
author = "Umićević, Sonja and Antić, Marina and Rajković, Dragana and Sinkovič, Lovro and Nagl, Nevena and Goreta Ban, Smiljana and Meglič, Vladimir and Todorović, Vida and Marjanović-Jeromela, Ana and Pipan, Barbara",
year = "2023",
abstract = "Leafy kale (Brassica oleracea var. acephala L.) is one of the most
important Brassicaceae species with health-promoting properties and great
diversity. To investigate variability within and among accessions, 12 accessions
(each accession comprised four individuals/genotypes) from the Balkan region
were genotyped at 12 simple sequence repeats (SSRs) loci. The selected SSR
markers originated from the genomes of B. oleracea (eight), B. rapa (two) and
B. napus (two). DNA was extracted from seeds, cotyledons, shoots, and young
leaves, depending on the germination energy of each seed, using magnetic
extraction method. Genetic analysis included eight accessions from Serbia (24
genotypes), two accessions from Montenegro (eight genotypes), and one
accession from Bosnia and Herzegovina and Croatia (with four genotypes each).
Accession-specific polymorphism was found at the Ol12-FO2 and Ol10-F11a
loci, where genotypes were qualitatively distinguished by horizontal agarose gel
electrophoresis (binary detection). Among 12 loci, the average polymorphic
information content (PIC) was 0.71, with the marker Ol13-C12 proving to be the
78 Umićević et al.
most informative (PIC = 0.89). However, the analysis of molecular variability
(AMOVA) showed 11% diversity between accessions, 19% diversity between
genotypes, and 70% diversity within genotypes, explaining the molecular
diversity of native Balkan leafy kale germplasm. According to the genetic
structure, four genetic groups were formed with an average expected
heterozygosity of 0.70 between clusters. In order to exploit the genetic diversity,
it would be advisable to evaluate these accessions at phenotypic level and use
their potential in breeding programmes., Raštan (Brassica oleracea var. acephala L.) je jedna od najvažnijih vrsta iz porodice kupusnjača koja povoljno utiče na zdravlje ljudi i odlikuje se velikom raznovrsnošću. Da bi se istražila varijabilnost unutar i između prinova, 12 prinova (svaka prinova se sastojala od četiri individue/genotipa) sa teritorije Balkanskog poluostrva je genotipizovano koristeći 12 SSR markera. Odabrani SSR markeri potiču iz genoma B. oleracea (osam), B. rapa (dva) i B. napus (dva). DNK je izolovana iz sjemena, kotiledona, izdanaka i mladih listova, u zavisnosti od energije klijanja svakog sjemena, metodom magnetne ekstrakcije. Genetičkom analizom obuhvaćeno je osam prinova iz Srbije (24 genotipa), dvije prinove iz Crne Gore (osam genotipova) i jedna prinova iz Bosne i Hercegovine i Hrvatske (sa po četiri genotipa). Polimorfizam specifičan za prinove pronađen je na lokusima Ol12-FO2 i Ol10-F11a, gdje su genotipovi kvalitativno razlikovani horizontalnom elektroforezom u agaroznom gelu (binarna detekcija). Kod ukupno 12 lokusa, prosječan sadržaj polimorfne informacije (PIC) bio je 0,71, pri čemu se kao najinformativniji pokazao marker Ol13-C12 (PIC = 0,89). Međutim, analiza molekularne varijabilnosti (AMOVA) pokazala je 11% raznovrsnosti između prinova, 19% raznovrsnosti između genotipova i 70% raznovrsnosti unutar genotipova, objašnjavajući molekularnu raznovrsnost izvorne germplazme raštana sa teritorije Balkanskog poluostrva. Prema genetičkoj strukturi formirane su četiri genetičke grupe sa prosječnom očekivanom heterozigotnošću od 0,70 između klastera. Da bi se iskoristio genetički diverzitet, bilo bi preporučljivo da se ove prinove karakterišu 90 Umićević et al. na fenotipskom nivou i da se njihov potencijal iskoristi u programima oplemenjivanja.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Agroznanje / Agro-knowledge Journal",
title = "Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.), Genetički diverzitet genetičkih resursa raštana (Brassica oleracea var. acephala L.)",
pages = "90-77",
number = "3",
volume = "24",
doi = "10.7251/AGREN2303077U"
}

Umićević, S., Antić, M., Rajković, D., Sinkovič, L., Nagl, N., Goreta Ban, S., Meglič, V., Todorović, V., Marjanović-Jeromela, A.,& Pipan, B.. (2023). Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.). in Agroznanje / Agro-knowledge Journal
Banja Luka : University of Banja Luka, Faculty of Agriculture., 24(3), 77-90.
https://doi.org/10.7251/AGREN2303077U

Umićević S, Antić M, Rajković D, Sinkovič L, Nagl N, Goreta Ban S, Meglič V, Todorović V, Marjanović-Jeromela A, Pipan B. Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.). in Agroznanje / Agro-knowledge Journal. 2023;24(3):77-90.
doi:10.7251/AGREN2303077U .

Umićević, Sonja, Antić, Marina, Rajković, Dragana, Sinkovič, Lovro, Nagl, Nevena, Goreta Ban, Smiljana, Meglič, Vladimir, Todorović, Vida, Marjanović-Jeromela, Ana, Pipan, Barbara, "Genetic diversity of leafy kale genetic resources (Brassica oleracea var. acephala L.)" in Agroznanje / Agro-knowledge Journal, 24, no. 3 (2023):77-90,
https://doi.org/10.7251/AGREN2303077U . .

TY  - JOUR
AU  - Nagl, Nevena
AU  - Sinkovič, Lovro
AU  - Savić, Aleksandra
AU  - Isakov, Milada
AU  - Hasanaklou, Hourieh Tavakoli
AU  - Pipan, Barbara
AU  - Marjanović-Jeromela, Ana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3788
AB  - Grass pea (Lathyrus sativus) is a valuable grain legume known for its high protein content and rich essential amino acid profile. Its exceptional characteristics such as drought tolerance, high adaptability to extreme conditions, disease resistance, and low cultivation inputs, make it particularly suitable for cultivation by resource-poor farmers. However, the potential use of grass pea is limited due to the presence of anti-nutritional factors, including protease inhibitors, especially trypsin inhibitor. This study aimed to develop a rapid and reliable method for measuring TI activity in seeds of grass pea and to investigate the influence of genotype and environment on trypsin inhibitor (TI) activity in seeds of grass pea. A set of 25 grass pea accessions from seven European countries was cultivated in Slovenia at the Agricultural Institute of Slovenia, and a set of 12 grass pea accessions from the Serbian gene bank was grown in Serbia at the Institute of Field and Vegetable Crops. The TI activity varied considerably among the grass pea accessions studied, with values ranging from 26.7 to 90.3 TUI/mg. To further evaluate the effects of environmental conditions on TI activity, eight grass pea accessions originating from Serbia were grown in both Slovenia and Serbia. The TI activity of the accessions grown in Slovenia ranged from 26.7 to 81.0 TUI/mg, while the activity of the accessions grown in Serbia ranged from 40.3 to 57.0 TUI/mg. The correlation of TI activity between grass pea accessions grown in Slovenia and those grown in Serbia was 0.39, with genotype diversity being the largest contributor (55.9%). This study provides a valuable insight into the variability of TI activity in grass pea and shows the possible influence of environmental conditions on this trait. However, since the data are only from a one-year field trial, further studies are needed to fully evaluate the influence of different environmental factors on TI activity.
PB  - Novi Sad : Institute of Field and Vegetable Crops
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.)
EP  - 39
IS  - 2
SP  - 32
VL  - 60
DO  - 10.5937/ratpov60-45934
ER  - 

@article{
author = "Nagl, Nevena and Sinkovič, Lovro and Savić, Aleksandra and Isakov, Milada and Hasanaklou, Hourieh Tavakoli and Pipan, Barbara and Marjanović-Jeromela, Ana",
year = "2023",
abstract = "Grass pea (Lathyrus sativus) is a valuable grain legume known for its high protein content and rich essential amino acid profile. Its exceptional characteristics such as drought tolerance, high adaptability to extreme conditions, disease resistance, and low cultivation inputs, make it particularly suitable for cultivation by resource-poor farmers. However, the potential use of grass pea is limited due to the presence of anti-nutritional factors, including protease inhibitors, especially trypsin inhibitor. This study aimed to develop a rapid and reliable method for measuring TI activity in seeds of grass pea and to investigate the influence of genotype and environment on trypsin inhibitor (TI) activity in seeds of grass pea. A set of 25 grass pea accessions from seven European countries was cultivated in Slovenia at the Agricultural Institute of Slovenia, and a set of 12 grass pea accessions from the Serbian gene bank was grown in Serbia at the Institute of Field and Vegetable Crops. The TI activity varied considerably among the grass pea accessions studied, with values ranging from 26.7 to 90.3 TUI/mg. To further evaluate the effects of environmental conditions on TI activity, eight grass pea accessions originating from Serbia were grown in both Slovenia and Serbia. The TI activity of the accessions grown in Slovenia ranged from 26.7 to 81.0 TUI/mg, while the activity of the accessions grown in Serbia ranged from 40.3 to 57.0 TUI/mg. The correlation of TI activity between grass pea accessions grown in Slovenia and those grown in Serbia was 0.39, with genotype diversity being the largest contributor (55.9%). This study provides a valuable insight into the variability of TI activity in grass pea and shows the possible influence of environmental conditions on this trait. However, since the data are only from a one-year field trial, further studies are needed to fully evaluate the influence of different environmental factors on TI activity.",
publisher = "Novi Sad : Institute of Field and Vegetable Crops",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.)",
pages = "39-32",
number = "2",
volume = "60",
doi = "10.5937/ratpov60-45934"
}

Nagl, N., Sinkovič, L., Savić, A., Isakov, M., Hasanaklou, H. T., Pipan, B.,& Marjanović-Jeromela, A.. (2023). Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.). in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Novi Sad : Institute of Field and Vegetable Crops., 60(2), 32-39.
https://doi.org/10.5937/ratpov60-45934

Nagl N, Sinkovič L, Savić A, Isakov M, Hasanaklou HT, Pipan B, Marjanović-Jeromela A. Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.). in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2023;60(2):32-39.
doi:10.5937/ratpov60-45934 .

Nagl, Nevena, Sinkovič, Lovro, Savić, Aleksandra, Isakov, Milada, Hasanaklou, Hourieh Tavakoli, Pipan, Barbara, Marjanović-Jeromela, Ana, "Trypsin inhibitor activity in grass pea seeds (Lathyrus sativus L.)" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 60, no. 2 (2023):32-39,
https://doi.org/10.5937/ratpov60-45934 . .

TY  - CONF
AU  - Umićević, Sonja
AU  - Antić, Marina
AU  - Rajković, Dragana
AU  - Sinkovič, Lovro
AU  - Nagl, Nevena
AU  - Goreta Ban, Smiljana
AU  - Meglič, Vladimir
AU  - Todorović, Vida
AU  - Marjanović-Jeromela, Ana
AU  - Pipan, Barbara
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4260
AB  - Leafy kale (Brassica oleracea var. acephala L.) is one of the most important Brassicaceae species with health-promoting properties and high diversity. To investigate variability within and between accessions, 12 accessions (each accession comprised four individuals/genotypes) from the Balkan area was genotyped at 12 SSR (Simple Sequence Repeats) loci. DNA was extracted from seeds, cotyledons, shoots, or young leaves, depending on the germination energy of each seed, using the MagMax (Applied Biosystems) magnetic extraction method with the BioSprint Plant Kit (Qiagen).
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 12th International Symposium on Agricultural Sciences "AgroReS 2023", 24-26 May 2023, Trebinje, Bosnia and Herzegovina
T1  - Molecular characterization of leafy kale genetic resources (Brassica oleracea var. acephala L.)
EP  - 189
SP  - 188
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4260
ER  - 

@conference{
author = "Umićević, Sonja and Antić, Marina and Rajković, Dragana and Sinkovič, Lovro and Nagl, Nevena and Goreta Ban, Smiljana and Meglič, Vladimir and Todorović, Vida and Marjanović-Jeromela, Ana and Pipan, Barbara",
year = "2023",
abstract = "Leafy kale (Brassica oleracea var. acephala L.) is one of the most important Brassicaceae species with health-promoting properties and high diversity. To investigate variability within and between accessions, 12 accessions (each accession comprised four individuals/genotypes) from the Balkan area was genotyped at 12 SSR (Simple Sequence Repeats) loci. DNA was extracted from seeds, cotyledons, shoots, or young leaves, depending on the germination energy of each seed, using the MagMax (Applied Biosystems) magnetic extraction method with the BioSprint Plant Kit (Qiagen).",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 12th International Symposium on Agricultural Sciences "AgroReS 2023", 24-26 May 2023, Trebinje, Bosnia and Herzegovina",
title = "Molecular characterization of leafy kale genetic resources (Brassica oleracea var. acephala L.)",
pages = "189-188",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4260"
}

Umićević, S., Antić, M., Rajković, D., Sinkovič, L., Nagl, N., Goreta Ban, S., Meglič, V., Todorović, V., Marjanović-Jeromela, A.,& Pipan, B.. (2023). Molecular characterization of leafy kale genetic resources (Brassica oleracea var. acephala L.). in Book of Abstracts, 12th International Symposium on Agricultural Sciences "AgroReS 2023", 24-26 May 2023, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 188-189.
https://hdl.handle.net/21.15107/rcub_fiver_4260

Umićević S, Antić M, Rajković D, Sinkovič L, Nagl N, Goreta Ban S, Meglič V, Todorović V, Marjanović-Jeromela A, Pipan B. Molecular characterization of leafy kale genetic resources (Brassica oleracea var. acephala L.). in Book of Abstracts, 12th International Symposium on Agricultural Sciences "AgroReS 2023", 24-26 May 2023, Trebinje, Bosnia and Herzegovina. 2023;:188-189.
https://hdl.handle.net/21.15107/rcub_fiver_4260 .

Umićević, Sonja, Antić, Marina, Rajković, Dragana, Sinkovič, Lovro, Nagl, Nevena, Goreta Ban, Smiljana, Meglič, Vladimir, Todorović, Vida, Marjanović-Jeromela, Ana, Pipan, Barbara, "Molecular characterization of leafy kale genetic resources (Brassica oleracea var. acephala L.)" in Book of Abstracts, 12th International Symposium on Agricultural Sciences "AgroReS 2023", 24-26 May 2023, Trebinje, Bosnia and Herzegovina (2023):188-189,
https://hdl.handle.net/21.15107/rcub_fiver_4260 .

TY  - CONF
AU  - Pipan, Barbara
AU  - Brezeanu, Creola
AU  - Petrova, Sofia
AU  - Sinkovič, Lovro
AU  - Antić, Marina
AU  - Bebeli, Penelope
AU  - De Carvalho, Miguel Angelo Pinheiro
AU  - Savić, Aleksandra
AU  - Marjanović-Jeromela, Ana
AU  - Strajeru, Silvia
AU  - Burlyaeva, Marina
AU  - Meglič, Vladimir
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4030
AB  - Grass pea (Lathyrus sativus L.) is a self-pollinating legume that is well adapted to changing climatic conditions. In our study, the collection of 25 accessions from different parts of Europe (7 from BiH, 2 from Romania, 4 from Portugal, 8 from Serbia, 2 from Bulgaria, 1 from Greece and 1 from Slovenia) was genetically assessed by 15 species-specific nSSR markers (Simple Sequence Repeat). Extraction of gDNA was performed from 3 to 10 individual young plants/genotypes of each accession using an automated magnetic procedure. PCR reaction mixtures and amplification were optimized for each primer pair under two-step touch-down PCR conditions. Fragment analysis was performed on a genetic analyzer (ABI 3130XL) together with the internal standard ROX500. Accurate allele lengths were recorded from the electropherograms in GeneMapper6.0 software. The diversity parameters and genetic structure of the Lathyrus collection was observed using various population genetic programmes such as Arleqin, MSToolkit, GenAlEx, Populations, TreeView, and Structure.
PB  - Edirne : Trakya University
C3  - Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya
T1  - Genetic structure and variability parameters of Lathyrus sativus L. European collection
EP  - 23
SP  - 23
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4030
ER  - 

@conference{
author = "Pipan, Barbara and Brezeanu, Creola and Petrova, Sofia and Sinkovič, Lovro and Antić, Marina and Bebeli, Penelope and De Carvalho, Miguel Angelo Pinheiro and Savić, Aleksandra and Marjanović-Jeromela, Ana and Strajeru, Silvia and Burlyaeva, Marina and Meglič, Vladimir",
year = "2023",
abstract = "Grass pea (Lathyrus sativus L.) is a self-pollinating legume that is well adapted to changing climatic conditions. In our study, the collection of 25 accessions from different parts of Europe (7 from BiH, 2 from Romania, 4 from Portugal, 8 from Serbia, 2 from Bulgaria, 1 from Greece and 1 from Slovenia) was genetically assessed by 15 species-specific nSSR markers (Simple Sequence Repeat). Extraction of gDNA was performed from 3 to 10 individual young plants/genotypes of each accession using an automated magnetic procedure. PCR reaction mixtures and amplification were optimized for each primer pair under two-step touch-down PCR conditions. Fragment analysis was performed on a genetic analyzer (ABI 3130XL) together with the internal standard ROX500. Accurate allele lengths were recorded from the electropherograms in GeneMapper6.0 software. The diversity parameters and genetic structure of the Lathyrus collection was observed using various population genetic programmes such as Arleqin, MSToolkit, GenAlEx, Populations, TreeView, and Structure.",
publisher = "Edirne : Trakya University",
journal = "Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya",
title = "Genetic structure and variability parameters of Lathyrus sativus L. European collection",
pages = "23-23",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4030"
}

Pipan, B., Brezeanu, C., Petrova, S., Sinkovič, L., Antić, M., Bebeli, P., De Carvalho, M. A. P., Savić, A., Marjanović-Jeromela, A., Strajeru, S., Burlyaeva, M.,& Meglič, V.. (2023). Genetic structure and variability parameters of Lathyrus sativus L. European collection. in Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya
Edirne : Trakya University., 23-23.
https://hdl.handle.net/21.15107/rcub_fiver_4030

Pipan B, Brezeanu C, Petrova S, Sinkovič L, Antić M, Bebeli P, De Carvalho MAP, Savić A, Marjanović-Jeromela A, Strajeru S, Burlyaeva M, Meglič V. Genetic structure and variability parameters of Lathyrus sativus L. European collection. in Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya. 2023;:23-23.
https://hdl.handle.net/21.15107/rcub_fiver_4030 .

Pipan, Barbara, Brezeanu, Creola, Petrova, Sofia, Sinkovič, Lovro, Antić, Marina, Bebeli, Penelope, De Carvalho, Miguel Angelo Pinheiro, Savić, Aleksandra, Marjanović-Jeromela, Ana, Strajeru, Silvia, Burlyaeva, Marina, Meglič, Vladimir, "Genetic structure and variability parameters of Lathyrus sativus L. European collection" in Proceedings, International Congress on Oil and Protein Crops, EUCARPIA Oil and Protein Crops Section, 2-4 November 2023, Antalya (2023):23-23,
https://hdl.handle.net/21.15107/rcub_fiver_4030 .

TY  - CONF
AU  - Toth, Viola
AU  - Takač, Verica
AU  - Sinkovič, Lovro
AU  - Meglič, Vladimir
AU  - Vida, Gyula
AU  - Rakszegi, Marianna
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2659
AB  - Wheat and other cereals are important sources of dietary fibre and antioxidants. Most of the bioactive components are found in the outer layers and aleurone layers of the seed, but also the flour contains some. The major dietary fibre components in the wheat grain are cell wall polysaccharides such as arabinoxylan and β-glucan, which account for about 70% and 20%, respectively, of the total cell wall polysaccharides in the starchy endosperm. Barley and oats are rich in β-glucan, while wheat and rye are richer in arabinoxylan. Arabinoxylan has two forms, i.e. water-soluble (WE) and insoluble (WU) fractions, which differ in their health benefits. Antioxidants (e.g., alkylresorcinol) delay or inhibit oxidation processes. Alkylresorcinol is formed in the organism and acts against free radicals, which are responsible for the cell degradation.
PB  - Tulln an der Donau : Universität für Bodenkultur Wien, Department für Nutzpflanzenwissenschaften, Institut für Pflanzenzüchtung
C3  - Proceedings, 72nd Conference "Plant Breeding for the Green Deal" / Tagungsband der 72. Jahrestagung der Vereinigung  der Pflanzenzüchter und Saatgutkaufleute Österreichs, 22-24 November 2021, Online Conference, Raumberg-Gumpenstein
T1  - Diversity of spelt and common wheat grown under different management based on their bioactive component composition
EP  - 80
SP  - 79
DO  - 10.5281/zenodo.5667799
ER  - 

@conference{
author = "Toth, Viola and Takač, Verica and Sinkovič, Lovro and Meglič, Vladimir and Vida, Gyula and Rakszegi, Marianna",
year = "2022",
abstract = "Wheat and other cereals are important sources of dietary fibre and antioxidants. Most of the bioactive components are found in the outer layers and aleurone layers of the seed, but also the flour contains some. The major dietary fibre components in the wheat grain are cell wall polysaccharides such as arabinoxylan and β-glucan, which account for about 70% and 20%, respectively, of the total cell wall polysaccharides in the starchy endosperm. Barley and oats are rich in β-glucan, while wheat and rye are richer in arabinoxylan. Arabinoxylan has two forms, i.e. water-soluble (WE) and insoluble (WU) fractions, which differ in their health benefits. Antioxidants (e.g., alkylresorcinol) delay or inhibit oxidation processes. Alkylresorcinol is formed in the organism and acts against free radicals, which are responsible for the cell degradation.",
publisher = "Tulln an der Donau : Universität für Bodenkultur Wien, Department für Nutzpflanzenwissenschaften, Institut für Pflanzenzüchtung",
journal = "Proceedings, 72nd Conference "Plant Breeding for the Green Deal" / Tagungsband der 72. Jahrestagung der Vereinigung  der Pflanzenzüchter und Saatgutkaufleute Österreichs, 22-24 November 2021, Online Conference, Raumberg-Gumpenstein",
title = "Diversity of spelt and common wheat grown under different management based on their bioactive component composition",
pages = "80-79",
doi = "10.5281/zenodo.5667799"
}

Toth, V., Takač, V., Sinkovič, L., Meglič, V., Vida, G.,& Rakszegi, M.. (2022). Diversity of spelt and common wheat grown under different management based on their bioactive component composition. in Proceedings, 72nd Conference "Plant Breeding for the Green Deal" / Tagungsband der 72. Jahrestagung der Vereinigung  der Pflanzenzüchter und Saatgutkaufleute Österreichs, 22-24 November 2021, Online Conference, Raumberg-Gumpenstein
Tulln an der Donau : Universität für Bodenkultur Wien, Department für Nutzpflanzenwissenschaften, Institut für Pflanzenzüchtung., 79-80.
https://doi.org/10.5281/zenodo.5667799

Toth V, Takač V, Sinkovič L, Meglič V, Vida G, Rakszegi M. Diversity of spelt and common wheat grown under different management based on their bioactive component composition. in Proceedings, 72nd Conference "Plant Breeding for the Green Deal" / Tagungsband der 72. Jahrestagung der Vereinigung  der Pflanzenzüchter und Saatgutkaufleute Österreichs, 22-24 November 2021, Online Conference, Raumberg-Gumpenstein. 2022;:79-80.
doi:10.5281/zenodo.5667799 .

Toth, Viola, Takač, Verica, Sinkovič, Lovro, Meglič, Vladimir, Vida, Gyula, Rakszegi, Marianna, "Diversity of spelt and common wheat grown under different management based on their bioactive component composition" in Proceedings, 72nd Conference "Plant Breeding for the Green Deal" / Tagungsband der 72. Jahrestagung der Vereinigung  der Pflanzenzüchter und Saatgutkaufleute Österreichs, 22-24 November 2021, Online Conference, Raumberg-Gumpenstein (2022):79-80,
https://doi.org/10.5281/zenodo.5667799 . .

TY  - CONF
AU  - Nagl, Nevena
AU  - Sinkovič, Lovro
AU  - Vasić, Mirjana
AU  - Meglič, Vladimir
AU  - Savić, Aleksandra
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Pipan, Barbara
AU  - Marjanović-Jeromela, Ana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2804
AB  - Grass pea (Lathyrus sativus L.) is a legume with high protein content and good quantities of essential amino acids. It has high tolerance to drought, high adaptability to extreme conditions, disease resistance and low input requirement for cultivation, which makes it very useful for cultivation by resource-poor farmers. Potential usage of grass pea is limited due to the presence of anti-nutritional factors including protease inhibitors, especially trypsin inhibitors. They have a major impact on nutritional value, by inhibiting pancreatic serine proteases (thus impairing protein digestion), but are also likely to protect legume seed against attack by predators. Although once extensively grown throughout Balkan Peninsula, grass pea was nearly forgotten. In recent years there was an effort to reintroduce and increase the interest of researchers and farmers in this crop. The aim of this study was to establish whether genotype and environment have significant influences on trypsin inhibitor activity (TIA) in grass pea and to determine whether TIA is correlated with seed protein content.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
T1  - Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity
EP  - 82
SP  - 82
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2804
ER  - 

@conference{
author = "Nagl, Nevena and Sinkovič, Lovro and Vasić, Mirjana and Meglič, Vladimir and Savić, Aleksandra and Nikolić, Zorica and Petrović, Gordana and Pipan, Barbara and Marjanović-Jeromela, Ana",
year = "2022",
abstract = "Grass pea (Lathyrus sativus L.) is a legume with high protein content and good quantities of essential amino acids. It has high tolerance to drought, high adaptability to extreme conditions, disease resistance and low input requirement for cultivation, which makes it very useful for cultivation by resource-poor farmers. Potential usage of grass pea is limited due to the presence of anti-nutritional factors including protease inhibitors, especially trypsin inhibitors. They have a major impact on nutritional value, by inhibiting pancreatic serine proteases (thus impairing protein digestion), but are also likely to protect legume seed against attack by predators. Although once extensively grown throughout Balkan Peninsula, grass pea was nearly forgotten. In recent years there was an effort to reintroduce and increase the interest of researchers and farmers in this crop. The aim of this study was to establish whether genotype and environment have significant influences on trypsin inhibitor activity (TIA) in grass pea and to determine whether TIA is correlated with seed protein content.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina",
title = "Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity",
pages = "82-82",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2804"
}

Nagl, N., Sinkovič, L., Vasić, M., Meglič, V., Savić, A., Nikolić, Z., Petrović, G., Pipan, B.,& Marjanović-Jeromela, A.. (2022). Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 82-82.
https://hdl.handle.net/21.15107/rcub_fiver_2804

Nagl N, Sinkovič L, Vasić M, Meglič V, Savić A, Nikolić Z, Petrović G, Pipan B, Marjanović-Jeromela A. Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina. 2022;:82-82.
https://hdl.handle.net/21.15107/rcub_fiver_2804 .

Nagl, Nevena, Sinkovič, Lovro, Vasić, Mirjana, Meglič, Vladimir, Savić, Aleksandra, Nikolić, Zorica, Petrović, Gordana, Pipan, Barbara, Marjanović-Jeromela, Ana, "Evaluation of Lathyrus sativus L. seed for protein content and trypsin inhibitor activity" in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina (2022):82-82,
https://hdl.handle.net/21.15107/rcub_fiver_2804 .

TY  - CONF
AU  - Pipan, Barbara
AU  - Antić, Marina
AU  - Rajković, Dragana
AU  - Sinkovič, Lovro
AU  - Rašeta, Sonja
AU  - Nagl, Nevena
AU  - Goreta Ban, Smiljana
AU  - Meglič, Vladimir
AU  - Todorović, Vida
AU  - Marjanović-Jeromela, Ana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2805
AB  - Family Brassicaceae is abundant in species used in food production, but also contains many industrial species, vegetables and ornamentals. Green collard (Brassica oleracea var. acephala L.) was traditionally grown in Balkan Peninsula and its wide green leaves, rich in vitamins A and C, are used for cooking. There is great diversity in B. oleracea acephala germplasm that reflects on agronomic, morphological and molecular characteristics. In this study, was assessed genetic variation of 38 accessions of green collard out of which 3 accessions were standard/commercial varieties and 35 were locally grown populations, collected in Western Balkan: 18 from Bosnia and Herzegovina, 8 from Serbia (Vojvodina), 7 from Croatia and two from Montenegro. Genetic diversity was assessed using twelve species-specific SSR markers (Simple Sequence Repeats) originating from B. oleracea (8), B. rapa (2) and B. napus (2). Each accession was represented with four individual samples.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
T1  - Assessment of molecular diversity of Brassica oleracea var. acephala
EP  - 138
SP  - 137
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2805
ER  - 

@conference{
author = "Pipan, Barbara and Antić, Marina and Rajković, Dragana and Sinkovič, Lovro and Rašeta, Sonja and Nagl, Nevena and Goreta Ban, Smiljana and Meglič, Vladimir and Todorović, Vida and Marjanović-Jeromela, Ana",
year = "2022",
abstract = "Family Brassicaceae is abundant in species used in food production, but also contains many industrial species, vegetables and ornamentals. Green collard (Brassica oleracea var. acephala L.) was traditionally grown in Balkan Peninsula and its wide green leaves, rich in vitamins A and C, are used for cooking. There is great diversity in B. oleracea acephala germplasm that reflects on agronomic, morphological and molecular characteristics. In this study, was assessed genetic variation of 38 accessions of green collard out of which 3 accessions were standard/commercial varieties and 35 were locally grown populations, collected in Western Balkan: 18 from Bosnia and Herzegovina, 8 from Serbia (Vojvodina), 7 from Croatia and two from Montenegro. Genetic diversity was assessed using twelve species-specific SSR markers (Simple Sequence Repeats) originating from B. oleracea (8), B. rapa (2) and B. napus (2). Each accession was represented with four individual samples.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina",
title = "Assessment of molecular diversity of Brassica oleracea var. acephala",
pages = "138-137",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2805"
}

Pipan, B., Antić, M., Rajković, D., Sinkovič, L., Rašeta, S., Nagl, N., Goreta Ban, S., Meglič, V., Todorović, V.,& Marjanović-Jeromela, A.. (2022). Assessment of molecular diversity of Brassica oleracea var. acephala. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 137-138.
https://hdl.handle.net/21.15107/rcub_fiver_2805

Pipan B, Antić M, Rajković D, Sinkovič L, Rašeta S, Nagl N, Goreta Ban S, Meglič V, Todorović V, Marjanović-Jeromela A. Assessment of molecular diversity of Brassica oleracea var. acephala. in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina. 2022;:137-138.
https://hdl.handle.net/21.15107/rcub_fiver_2805 .

Pipan, Barbara, Antić, Marina, Rajković, Dragana, Sinkovič, Lovro, Rašeta, Sonja, Nagl, Nevena, Goreta Ban, Smiljana, Meglič, Vladimir, Todorović, Vida, Marjanović-Jeromela, Ana, "Assessment of molecular diversity of Brassica oleracea var. acephala" in Book of Abstracts, 11th International Symposium of Agricultural Sciences “AgroReS 2022", 26-28 May 2022, Trebinje, Bosnia and Herzegovina (2022):137-138,
https://hdl.handle.net/21.15107/rcub_fiver_2805 .

TY  - CONF
AU  - Sinkovič, Lovro
AU  - Pipan, Barbara
AU  - Meglič, Vladimir
AU  - Petrova, Sofija
AU  - Antić, Marina
AU  - Bebeli, Penelope
AU  - Pinheiro de Carvalho, Miguel Ângelo
AU  - Savić, Aleksandra
AU  - Vasić, Mirjana
AU  - Brezeanu, Creola
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2376
AB  - The ECPGR (European Cooperative Programme for Plant Genetic Resources) Grain legumes working group cover a range of species, which have enormous benefits for environment, food security and as well as the breeding material. However, despite its huge potential lots of species, including Lathyrus sp., are not well represented in European collection and enough exploited for different purposes. As starting point of EUGrainLeg project the activities act in two directions: i) AEGIS progress and improved data on European grain legumes plant genetic resources, and ii) improvement of group strategy work – in view of subgroup establishment based on species interest, in order to facilitate experience and knowledge exchange. The task of establishing the European grain legume collection needs updates and improvements, taking in account the large number of species managed inside the group. The significant diversity of Lathyrus sp. exist, but there are difficulties/gaps in order to identify accessions as AEGIS accessions, recording to registered data.
PB  - Banja Luka : University of Banja Luka, Faculty of Agriculture
C3  - Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina
T1  - Lathyrus diversity: available resources with relevance to crop improvement – progress reviews and relationship with AEGIS (EUGrainLeg)
EP  - 62
SP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2376
ER  - 

@conference{
author = "Sinkovič, Lovro and Pipan, Barbara and Meglič, Vladimir and Petrova, Sofija and Antić, Marina and Bebeli, Penelope and Pinheiro de Carvalho, Miguel Ângelo and Savić, Aleksandra and Vasić, Mirjana and Brezeanu, Creola",
year = "2020",
abstract = "The ECPGR (European Cooperative Programme for Plant Genetic Resources) Grain legumes working group cover a range of species, which have enormous benefits for environment, food security and as well as the breeding material. However, despite its huge potential lots of species, including Lathyrus sp., are not well represented in European collection and enough exploited for different purposes. As starting point of EUGrainLeg project the activities act in two directions: i) AEGIS progress and improved data on European grain legumes plant genetic resources, and ii) improvement of group strategy work – in view of subgroup establishment based on species interest, in order to facilitate experience and knowledge exchange. The task of establishing the European grain legume collection needs updates and improvements, taking in account the large number of species managed inside the group. The significant diversity of Lathyrus sp. exist, but there are difficulties/gaps in order to identify accessions as AEGIS accessions, recording to registered data.",
publisher = "Banja Luka : University of Banja Luka, Faculty of Agriculture",
journal = "Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina",
title = "Lathyrus diversity: available resources with relevance to crop improvement – progress reviews and relationship with AEGIS (EUGrainLeg)",
pages = "62-61",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2376"
}

Sinkovič, L., Pipan, B., Meglič, V., Petrova, S., Antić, M., Bebeli, P., Pinheiro de Carvalho, M. Â., Savić, A., Vasić, M.,& Brezeanu, C.. (2020). Lathyrus diversity: available resources with relevance to crop improvement – progress reviews and relationship with AEGIS (EUGrainLeg). in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina
Banja Luka : University of Banja Luka, Faculty of Agriculture., 61-62.
https://hdl.handle.net/21.15107/rcub_fiver_2376

Sinkovič L, Pipan B, Meglič V, Petrova S, Antić M, Bebeli P, Pinheiro de Carvalho MÂ, Savić A, Vasić M, Brezeanu C. Lathyrus diversity: available resources with relevance to crop improvement – progress reviews and relationship with AEGIS (EUGrainLeg). in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina. 2020;:61-62.
https://hdl.handle.net/21.15107/rcub_fiver_2376 .

Sinkovič, Lovro, Pipan, Barbara, Meglič, Vladimir, Petrova, Sofija, Antić, Marina, Bebeli, Penelope, Pinheiro de Carvalho, Miguel Ângelo, Savić, Aleksandra, Vasić, Mirjana, Brezeanu, Creola, "Lathyrus diversity: available resources with relevance to crop improvement – progress reviews and relationship with AEGIS (EUGrainLeg)" in Book of Abstracts, 9th International Symposium on Agricultural Sciences "AgroReS 2020", 24 September 2020, Banja Luka, Bosnia and Herzegovina (2020):61-62,
https://hdl.handle.net/21.15107/rcub_fiver_2376 .