TY  - CONF
AU  - Kiprovski, Biljana
AU  - Malenčić, Djordje
AU  - Ljubojević, Mirjana
AU  - Ognjanov, Vladislav
AU  - Veberič, Robert
AU  - Mikulič-Petkovšek, Maja
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2868
AB  - Compounds which add to bioactivity of elderberry (Sambucus nigra L.) are mostly phenolic compounds. One wild edible and two cultivated genotypes (’Haschberg’ and ’Ljubostinja’ selections) have been chosen. Leaves and fruits were collected in four stages (April-September). Total phenolics (TP) content was determined from MeOH extracts by Folin-Ciocalteau method while determination of individual compounds was performed using HPLC-DAD-ESI-MSn analysis with a DAD at 280, 350 and 530 nm. TP content in leaves varied through developmental stages, being higher in II and III stage. Leaves and fruits (both unripe and ripe) of ’Ljubostinja’ plants had higher content of TP compared to wild ripe fruits (1.5-fold). Leaves had caffeic acid derivatives (0.3-0.7% in wild, 0.2-0.3% in ’Haschberg’ and 0.4-0.6% in ’Ljubostinja’ in dry weigh) and caffeoylquinic acid derivatives, while fruits had more caffeoylquinic acid derivatives, particularly unripe fruits. The major phenolic acid present in leaves, especially during the II stage of development, was chlorogenic acid (approx. 4% dw) and the same was recorded in fruits (0.03% dw in unripe fruits of wild plants). Content of phenolic acids decreased during ripening of elderberry fruits and the similar was detected in the leaves.
PB  - Niš : University of Niš, Faculty of Sciences and Mathematics
PB  - Belgrade : Institute for Nature Conservation of Serbia
C3  - Abstracts, 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, 26-29 June 2022
T1  - Total and individual phenolics in wild and cultivated elderberry during development
EP  - 127
SP  - 127
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2868
ER  - 

@conference{
author = "Kiprovski, Biljana and Malenčić, Djordje and Ljubojević, Mirjana and Ognjanov, Vladislav and Veberič, Robert and Mikulič-Petkovšek, Maja",
year = "2022",
abstract = "Compounds which add to bioactivity of elderberry (Sambucus nigra L.) are mostly phenolic compounds. One wild edible and two cultivated genotypes (’Haschberg’ and ’Ljubostinja’ selections) have been chosen. Leaves and fruits were collected in four stages (April-September). Total phenolics (TP) content was determined from MeOH extracts by Folin-Ciocalteau method while determination of individual compounds was performed using HPLC-DAD-ESI-MSn analysis with a DAD at 280, 350 and 530 nm. TP content in leaves varied through developmental stages, being higher in II and III stage. Leaves and fruits (both unripe and ripe) of ’Ljubostinja’ plants had higher content of TP compared to wild ripe fruits (1.5-fold). Leaves had caffeic acid derivatives (0.3-0.7% in wild, 0.2-0.3% in ’Haschberg’ and 0.4-0.6% in ’Ljubostinja’ in dry weigh) and caffeoylquinic acid derivatives, while fruits had more caffeoylquinic acid derivatives, particularly unripe fruits. The major phenolic acid present in leaves, especially during the II stage of development, was chlorogenic acid (approx. 4% dw) and the same was recorded in fruits (0.03% dw in unripe fruits of wild plants). Content of phenolic acids decreased during ripening of elderberry fruits and the similar was detected in the leaves.",
publisher = "Niš : University of Niš, Faculty of Sciences and Mathematics, Belgrade : Institute for Nature Conservation of Serbia",
journal = "Abstracts, 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, 26-29 June 2022",
title = "Total and individual phenolics in wild and cultivated elderberry during development",
pages = "127-127",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2868"
}

Kiprovski, B., Malenčić, D., Ljubojević, M., Ognjanov, V., Veberič, R.,& Mikulič-Petkovšek, M.. (2022). Total and individual phenolics in wild and cultivated elderberry during development. in Abstracts, 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, 26-29 June 2022
Niš : University of Niš, Faculty of Sciences and Mathematics., 127-127.
https://hdl.handle.net/21.15107/rcub_fiver_2868

Kiprovski B, Malenčić D, Ljubojević M, Ognjanov V, Veberič R, Mikulič-Petkovšek M. Total and individual phenolics in wild and cultivated elderberry during development. in Abstracts, 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, 26-29 June 2022. 2022;:127-127.
https://hdl.handle.net/21.15107/rcub_fiver_2868 .

Kiprovski, Biljana, Malenčić, Djordje, Ljubojević, Mirjana, Ognjanov, Vladislav, Veberič, Robert, Mikulič-Petkovšek, Maja, "Total and individual phenolics in wild and cultivated elderberry during development" in Abstracts, 14th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, Kladovo, 26-29 June 2022 (2022):127-127,
https://hdl.handle.net/21.15107/rcub_fiver_2868 .

TY  - JOUR
AU  - Kiprovski, Biljana
AU  - Malenčić, Ðorđe
AU  - Ljubojević, Mirjana
AU  - Ognjanov, Vladislav
AU  - Veberić, Robert
AU  - Hudina, Metka
AU  - Mikulić-Petkovšek, Maja
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2096
AB  - This is the first report on the difference in the contents of the major quality compounds during development among cultivated ('Haschberg' cultivar and 'Ljubostinja' selection) and wild edible elderberry plants. Phenolics content in leaves (four stages) and fruits (unripe and ripe), as well as organic acids and sugars content in unripe and ripe fruits were determined. Quinic acid was the major organic acid in unripe fruits (especially in 'Wild' plants 16.7 g 100 g(-1)), while ripe fruits accumulated citric acid in the highest amounts (the highest was in' Ljubostinja' 10.2 g 100 g(-1)). 'Wild' elderberry plants had significantly higher sugar content in fruits, in general (2.2 unripe and in ripe 8.4 g 100 g(-1)), except for fructose content which was the highest in' Ljubostinja' ripe fruits (up to 6-fold higher). Tested elderberry plants had four major groups of phenolics in their leaves and fruits during development: phenolic acids (caffeic and caffeoylquinic acid derivatives), flavanone, flavonols (quercetin, kaempferol and isorhamnetin derivatives) and anthocyanins (mostly cyanidin derivatives).' Ljubostinja' had higher content of cyanidin derivatives in leaves in flowering stage and in all tested fruits (0.01% DW in unripe and 0.7% DW in ripe). Bearing in mind all tested quality parameters, 'Ljubostinja' selection had better quality of fruits, which position this selection as valuable resource for further cultivation and breeding of elderberry.
PB  - Elsevier, Amsterdam
T2  - Scientia Horticulturae
T1  - Quality parameters change during ripening in leaves and fruits of wild growing and cultivated elderberry (Sambucus nigra) genotypes
VL  - 277
DO  - 10.1016/j.scienta.2020.109792
ER  - 

@article{
author = "Kiprovski, Biljana and Malenčić, Ðorđe and Ljubojević, Mirjana and Ognjanov, Vladislav and Veberić, Robert and Hudina, Metka and Mikulić-Petkovšek, Maja",
year = "2021",
abstract = "This is the first report on the difference in the contents of the major quality compounds during development among cultivated ('Haschberg' cultivar and 'Ljubostinja' selection) and wild edible elderberry plants. Phenolics content in leaves (four stages) and fruits (unripe and ripe), as well as organic acids and sugars content in unripe and ripe fruits were determined. Quinic acid was the major organic acid in unripe fruits (especially in 'Wild' plants 16.7 g 100 g(-1)), while ripe fruits accumulated citric acid in the highest amounts (the highest was in' Ljubostinja' 10.2 g 100 g(-1)). 'Wild' elderberry plants had significantly higher sugar content in fruits, in general (2.2 unripe and in ripe 8.4 g 100 g(-1)), except for fructose content which was the highest in' Ljubostinja' ripe fruits (up to 6-fold higher). Tested elderberry plants had four major groups of phenolics in their leaves and fruits during development: phenolic acids (caffeic and caffeoylquinic acid derivatives), flavanone, flavonols (quercetin, kaempferol and isorhamnetin derivatives) and anthocyanins (mostly cyanidin derivatives).' Ljubostinja' had higher content of cyanidin derivatives in leaves in flowering stage and in all tested fruits (0.01% DW in unripe and 0.7% DW in ripe). Bearing in mind all tested quality parameters, 'Ljubostinja' selection had better quality of fruits, which position this selection as valuable resource for further cultivation and breeding of elderberry.",
publisher = "Elsevier, Amsterdam",
journal = "Scientia Horticulturae",
title = "Quality parameters change during ripening in leaves and fruits of wild growing and cultivated elderberry (Sambucus nigra) genotypes",
volume = "277",
doi = "10.1016/j.scienta.2020.109792"
}

Kiprovski, B., Malenčić, Ð., Ljubojević, M., Ognjanov, V., Veberić, R., Hudina, M.,& Mikulić-Petkovšek, M.. (2021). Quality parameters change during ripening in leaves and fruits of wild growing and cultivated elderberry (Sambucus nigra) genotypes. in Scientia Horticulturae
Elsevier, Amsterdam., 277.
https://doi.org/10.1016/j.scienta.2020.109792

Kiprovski B, Malenčić Ð, Ljubojević M, Ognjanov V, Veberić R, Hudina M, Mikulić-Petkovšek M. Quality parameters change during ripening in leaves and fruits of wild growing and cultivated elderberry (Sambucus nigra) genotypes. in Scientia Horticulturae. 2021;277.
doi:10.1016/j.scienta.2020.109792 .

Kiprovski, Biljana, Malenčić, Ðorđe, Ljubojević, Mirjana, Ognjanov, Vladislav, Veberić, Robert, Hudina, Metka, Mikulić-Petkovšek, Maja, "Quality parameters change during ripening in leaves and fruits of wild growing and cultivated elderberry (Sambucus nigra) genotypes" in Scientia Horticulturae, 277 (2021),
https://doi.org/10.1016/j.scienta.2020.109792 . .

TY  - CONF
AU  - Dorić, Dušica
AU  - Malenčić, Djordje
AU  - Kiprovski, Biljana
AU  - Barać, Goran
AU  - Ljubojević, Mirjana
AU  - Ognjanov, Vladislav
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/2326
AB  - Oxidative metabolism of normal cells and different stress situations generate highly reactive oxygen
species (ROS). The ROS have been implicated in a number of physiological disorders in plants. Such factors as certain mechanical injuries are known to induce ROS formation in most aerobic organisms. Antioxidant defence systems have co-evolved with aerobic metabolism to counteract oxidative damage from ROS. This includes antioxidant enzymes such as superoxide-dismutase (SOD; EC 1.15.1.1). SOD is of the greatest importance since it catalyzes the dismutation of the superoxide radical (O2.-) to O2 and H2O. Thus, the aim of this study was to measure the intensity of SOD in the leaves of six cherry rootstock selections in order to investigate the effect of exogenously applied auxins on the mechanical injury induced oxidative stress during the process of rooting of softwood cuttings.
The experiment was carried out in the plastic house under a fogging system with 95-99% average relative
humidity. The 15-20 cm long terminal cuttings were treated with the mix of auxins (IBA, 0.5% and α-NAA, 0.8%)
powder prior to insertion into the white sphagnum and perlite substrate mixture. Leaves of six promising rootstock selections of Prunus cerasus L., P. mahaleb L. and P. fruticosa Pall., and one standard rootstock PHL-A were sampled on the 1st, 3rd and 7th day after inserting softwood cuttings into the rooting substrate. The SOD activity was measured by monitoring the inhibition of nitroblue-tetrazolium (NBT) reduction at 560 nm.
Results showed that generation of ROS occurred in all investigated genotypes, but they greatly differed in
susceptibility towards oxidative stress induces by mechanical injury. Application of auxins on standard vegetative rootstock PHL-A, as well as P. mahaleb selection M6 showed positive effect on lowering SOD activity after the 3rd and 7th collecting day compared to control. On the other hand, selections of P. cerasus OV22 and P. fruticosa SV2 expressed high SOD activity during all collecting days which was from 3.6-54.9% higher compared to control.
Enhanced SOD activity in leaves of softwood cuttings indicates induction of the enzymatic antioxidant system in
both auxin treated and untreated cutting. According to these results SOD activity could be used as one of the
biochemical parameters in further rootstock selection for sweet and sour cherries.
PB  - International Organization for Biological and Integrated Control (IOBC)
C3  - Book of Abstracts, 9th International Conference on Integrated Fruit Production, 04-08.09.2016, Thessaloniki, Greece
T1  - Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress
EP  - 167
SP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2326
ER  - 

@conference{
author = "Dorić, Dušica and Malenčić, Djordje and Kiprovski, Biljana and Barać, Goran and Ljubojević, Mirjana and Ognjanov, Vladislav",
year = "2016",
abstract = "Oxidative metabolism of normal cells and different stress situations generate highly reactive oxygen
species (ROS). The ROS have been implicated in a number of physiological disorders in plants. Such factors as certain mechanical injuries are known to induce ROS formation in most aerobic organisms. Antioxidant defence systems have co-evolved with aerobic metabolism to counteract oxidative damage from ROS. This includes antioxidant enzymes such as superoxide-dismutase (SOD; EC 1.15.1.1). SOD is of the greatest importance since it catalyzes the dismutation of the superoxide radical (O2.-) to O2 and H2O. Thus, the aim of this study was to measure the intensity of SOD in the leaves of six cherry rootstock selections in order to investigate the effect of exogenously applied auxins on the mechanical injury induced oxidative stress during the process of rooting of softwood cuttings.
The experiment was carried out in the plastic house under a fogging system with 95-99% average relative
humidity. The 15-20 cm long terminal cuttings were treated with the mix of auxins (IBA, 0.5% and α-NAA, 0.8%)
powder prior to insertion into the white sphagnum and perlite substrate mixture. Leaves of six promising rootstock selections of Prunus cerasus L., P. mahaleb L. and P. fruticosa Pall., and one standard rootstock PHL-A were sampled on the 1st, 3rd and 7th day after inserting softwood cuttings into the rooting substrate. The SOD activity was measured by monitoring the inhibition of nitroblue-tetrazolium (NBT) reduction at 560 nm.
Results showed that generation of ROS occurred in all investigated genotypes, but they greatly differed in
susceptibility towards oxidative stress induces by mechanical injury. Application of auxins on standard vegetative rootstock PHL-A, as well as P. mahaleb selection M6 showed positive effect on lowering SOD activity after the 3rd and 7th collecting day compared to control. On the other hand, selections of P. cerasus OV22 and P. fruticosa SV2 expressed high SOD activity during all collecting days which was from 3.6-54.9% higher compared to control.
Enhanced SOD activity in leaves of softwood cuttings indicates induction of the enzymatic antioxidant system in
both auxin treated and untreated cutting. According to these results SOD activity could be used as one of the
biochemical parameters in further rootstock selection for sweet and sour cherries.",
publisher = "International Organization for Biological and Integrated Control (IOBC)",
journal = "Book of Abstracts, 9th International Conference on Integrated Fruit Production, 04-08.09.2016, Thessaloniki, Greece",
title = "Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress",
pages = "167-167",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2326"
}

Dorić, D., Malenčić, D., Kiprovski, B., Barać, G., Ljubojević, M.,& Ognjanov, V.. (2016). Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress. in Book of Abstracts, 9th International Conference on Integrated Fruit Production, 04-08.09.2016, Thessaloniki, Greece
International Organization for Biological and Integrated Control (IOBC)., 167-167.
https://hdl.handle.net/21.15107/rcub_fiver_2326

Dorić D, Malenčić D, Kiprovski B, Barać G, Ljubojević M, Ognjanov V. Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress. in Book of Abstracts, 9th International Conference on Integrated Fruit Production, 04-08.09.2016, Thessaloniki, Greece. 2016;:167-167.
https://hdl.handle.net/21.15107/rcub_fiver_2326 .

Dorić, Dušica, Malenčić, Djordje, Kiprovski, Biljana, Barać, Goran, Ljubojević, Mirjana, Ognjanov, Vladislav, "Changes in superoxide-dismutase (SOD) activity in auxin treated Prunus sp. softwood cuttings under oxidative stress" in Book of Abstracts, 9th International Conference on Integrated Fruit Production, 04-08.09.2016, Thessaloniki, Greece (2016):167-167,
https://hdl.handle.net/21.15107/rcub_fiver_2326 .

TY  - JOUR
AU  - Dorić, Dušica
AU  - Kiprovski, Biljana
AU  - Malenčić, Đorđe
AU  - Ognjanov, Vladislav
AU  - Ljubojević, Mirjana
AU  - Barać, Goran
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/2210
AB  - The effect of exogenously applied auxins on mechanical injury induced oxidative stress was studied in 6 promising rootstock selections of Prunus cerasus L., P. mahaleb L., and P. fruticosa Pall. Investigated selections were included in low vigorous rootstock breeding programme for sweet and sour cherries. The standard rootstock PHL-A was used as a control, due to successful rooting. Leaves of investigated rootstocks were collected on: 0, 1st, 3rd and 7th day of inserting softwood cuttings into the rooting substrate under a fogging system with 95-99% average relative humidity. Exogenously applied auxin mixture consisted of 0.8% α-naphthylacetic acid (NAA) and 0.5% indolebutyric acid (IBA). Mechanical injuries during rooting period represent one of the factors that induce stress in softwood cuttings. Intensity of LP is used as a valuable biomarker of plant response to various abiotic factors. Differences in intensity of LP between auxin-treated and -untreated cuttings were examined. Almost all of investigated selections had lower LP intensity after auxin application (11.4-47.2%) between 1st and 3rd day. However, the most prominent change was in leaves of PHL-A (49.2%). The best LP-lowering effect were recorded in leaves of P. fruticosa, SV4 selection (56.9%) on 7th day and in P. mahaleb, M4 selection, on 1st and 3rd day (5.9, 5.8%), in comparison to untreated softwood cuttings. As for OV21 selection, LP intensity significantly increased in both treated and untreated cuttings on the 1st, 3rd and 7th day, but auxin-treated cuttings showed lower LP values, except on 7th day, reaching 221.3 nmol MDA equivalents g-1 fresh weight. Intensity of LP during vegetative propagation of selected genotypes could be used as one of the biochemical parameters in further rootstock selection for sweet and sour cherries.
PB  - Novi Sad : University of Novi Sad, Faculty of Agriculture
T2  - Contemporary Agriculture
T1  - Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins
T1  - Smanjenje intenziteta lipidne peroksidacije u listovima selekcija vegetativnih podloga za višnju i trešnju upotrebom egzogeno primenjenih auksina
EP  - 85
IS  - 1-2
SP  - 78
VL  - 64
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2210
ER  - 

@article{
author = "Dorić, Dušica and Kiprovski, Biljana and Malenčić, Đorđe and Ognjanov, Vladislav and Ljubojević, Mirjana and Barać, Goran",
year = "2015",
abstract = "The effect of exogenously applied auxins on mechanical injury induced oxidative stress was studied in 6 promising rootstock selections of Prunus cerasus L., P. mahaleb L., and P. fruticosa Pall. Investigated selections were included in low vigorous rootstock breeding programme for sweet and sour cherries. The standard rootstock PHL-A was used as a control, due to successful rooting. Leaves of investigated rootstocks were collected on: 0, 1st, 3rd and 7th day of inserting softwood cuttings into the rooting substrate under a fogging system with 95-99% average relative humidity. Exogenously applied auxin mixture consisted of 0.8% α-naphthylacetic acid (NAA) and 0.5% indolebutyric acid (IBA). Mechanical injuries during rooting period represent one of the factors that induce stress in softwood cuttings. Intensity of LP is used as a valuable biomarker of plant response to various abiotic factors. Differences in intensity of LP between auxin-treated and -untreated cuttings were examined. Almost all of investigated selections had lower LP intensity after auxin application (11.4-47.2%) between 1st and 3rd day. However, the most prominent change was in leaves of PHL-A (49.2%). The best LP-lowering effect were recorded in leaves of P. fruticosa, SV4 selection (56.9%) on 7th day and in P. mahaleb, M4 selection, on 1st and 3rd day (5.9, 5.8%), in comparison to untreated softwood cuttings. As for OV21 selection, LP intensity significantly increased in both treated and untreated cuttings on the 1st, 3rd and 7th day, but auxin-treated cuttings showed lower LP values, except on 7th day, reaching 221.3 nmol MDA equivalents g-1 fresh weight. Intensity of LP during vegetative propagation of selected genotypes could be used as one of the biochemical parameters in further rootstock selection for sweet and sour cherries.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Agriculture",
journal = "Contemporary Agriculture",
title = "Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins, Smanjenje intenziteta lipidne peroksidacije u listovima selekcija vegetativnih podloga za višnju i trešnju upotrebom egzogeno primenjenih auksina",
pages = "85-78",
number = "1-2",
volume = "64",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2210"
}

Dorić, D., Kiprovski, B., Malenčić, Đ., Ognjanov, V., Ljubojević, M.,& Barać, G.. (2015). Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins. in Contemporary Agriculture
Novi Sad : University of Novi Sad, Faculty of Agriculture., 64(1-2), 78-85.
https://hdl.handle.net/21.15107/rcub_fiver_2210

Dorić D, Kiprovski B, Malenčić Đ, Ognjanov V, Ljubojević M, Barać G. Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins. in Contemporary Agriculture. 2015;64(1-2):78-85.
https://hdl.handle.net/21.15107/rcub_fiver_2210 .

Dorić, Dušica, Kiprovski, Biljana, Malenčić, Đorđe, Ognjanov, Vladislav, Ljubojević, Mirjana, Barać, Goran, "Mitigating lipid peroxidation in leaves of Prunus sp. rootstocks using exogenously applied auxins" in Contemporary Agriculture, 64, no. 1-2 (2015):78-85,
https://hdl.handle.net/21.15107/rcub_fiver_2210 .

TY  - JOUR
AU  - Malenčić, Đorđe
AU  - Prvulović, Dejan
AU  - Popović, Milan
AU  - Ognjanov, Vladislav
AU  - Ljubojević, Mirjana
AU  - Barać, Goran
AU  - Borković, Boško
AU  - Kevrešan, Slavko
AU  - Kiprovski, Biljana
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/2213
AB  - In order to investigate the effect of phytohormones exogenous auxins on the level of the mechanical injury induced oxidative stress, intensity of lipid peroxidation (LP) was measured in rootstocks of cherry softwood cuttings. Basal part of the cuttings and leaves of five rootstocks (Mahaleb 1 and 2, Gisela 5, European ground cherry (EGC) and “Oblačinska” sour cherry) were sampled 0, 2, 4 and 6 days after cutting. Cuttings were treated with 0.5% solutions of three auxins: α-naphthylacetic acid (NAA), indolebutyric acid (IBA) and combination of these two (INCIT K). Results obtained for the LP intensity varied depending on the rootstock, plant organ and auxin applied. The best LP-lowering effect of auxins occurred in all genotypes when treated with NAA. In the leaves, the LP peaked on the 2nd day, while the best LP-lowering effect was recorded in European ground cherry rootstocks on the 4th day, independently of auxin applied. The highest MDA production in leaves was recorded in Mahaleb 2 and European ground cherry rootstocks treated with IBA (60-90% higher than control) two days after the cuttings were made. It has been established that in the most of the rootstocks examined auxins showed lowering effect on LP which points to their positive effect, not only on rooting of softwood cuttings, but on their antioxidant protection system, as well.
AB  - U radu je ispitan nivo oksidativnog stresa izazvanog mehaničkom povredom biljke prilikom proizvodnje reznica, u bazalnom delu i listovima pet podloga za višnju i trešnju (Magriva 1 i 2, Gizela 5, Stepska (EGC) i “Oblačinska” višnja). Istovremeno, reznice su tretirane 0.5% rastvorima tri auksina (α-naftilsirćetna kiselina, NAA, indol- buterna kiselina, IBA i kombinacija ovih hormona, INCIT K), u periodu od 60 min, kako bi se utvrdio efekat auksina na antioksidantni status reznica višnje. Uzorci su uzimani nakon 0, 2, 4 i 6 dana. Kontrolu su činile reznice držane u vodi bez hormona. Intenzitet lipidne peroksidacije (LP) je meren kao produkcija malondialdehida (MDA), spektrofotometrijski na 532 nm, i izražen je u nmol MDA g-1 sveže mase.
Dobijeni rezultati su varirali u zavisnosti od podloge, organa biljke i primenjenih auksina. Utvrđeno je da je intenzitet LP u bazalnom delu bio najveći prvog dana, na- kon ozleđivanja, a da su svi ispitivani auksini snižavali produkciju MDA u narednim danima. Najbolji efekat pokazala je Magriva 1 tretirana sa NAA. U listovima, LP je bila najveća 2. dana, a najbolji efekat na smanjenje pokazali su primenjeni auksini u Stepskoj višnji (4. dana). Najveća produkcija MDA u listovima zabeležena je u Magrivoj 2 i Stepskoj višnji tretiranih sa IBA, 2. dana nakon odsecanja reznica (60-90% više od kontrole). Utvrđeno je da u većini podloga pod dejstvom ispitivanih hormona dolazi do smanjenja intenziteta LP što ukazuje na pozitivan efekat primenjenih auksina, ne samo na ožiljavanje reznica nego i na njihov antioksidantni sistem.
PB  - Novi Sad : University of Novi Sad, Faculty of Agriculture
T2  - Contemporary Agriculture
T1  - Changes in the lipid peroxidation intensity in auxin treated cherry rootstocks softwood cuttings
T1  - Promene intenziteta lipidne peroksidacije u reznicama podloga za višnju i trešnju tretiranim auksinima
EP  - 229
IS  - 3-4
SP  - 221
VL  - 61
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2213
ER  - 

@article{
author = "Malenčić, Đorđe and Prvulović, Dejan and Popović, Milan and Ognjanov, Vladislav and Ljubojević, Mirjana and Barać, Goran and Borković, Boško and Kevrešan, Slavko and Kiprovski, Biljana",
year = "2012",
abstract = "In order to investigate the effect of phytohormones exogenous auxins on the level of the mechanical injury induced oxidative stress, intensity of lipid peroxidation (LP) was measured in rootstocks of cherry softwood cuttings. Basal part of the cuttings and leaves of five rootstocks (Mahaleb 1 and 2, Gisela 5, European ground cherry (EGC) and “Oblačinska” sour cherry) were sampled 0, 2, 4 and 6 days after cutting. Cuttings were treated with 0.5% solutions of three auxins: α-naphthylacetic acid (NAA), indolebutyric acid (IBA) and combination of these two (INCIT K). Results obtained for the LP intensity varied depending on the rootstock, plant organ and auxin applied. The best LP-lowering effect of auxins occurred in all genotypes when treated with NAA. In the leaves, the LP peaked on the 2nd day, while the best LP-lowering effect was recorded in European ground cherry rootstocks on the 4th day, independently of auxin applied. The highest MDA production in leaves was recorded in Mahaleb 2 and European ground cherry rootstocks treated with IBA (60-90% higher than control) two days after the cuttings were made. It has been established that in the most of the rootstocks examined auxins showed lowering effect on LP which points to their positive effect, not only on rooting of softwood cuttings, but on their antioxidant protection system, as well., U radu je ispitan nivo oksidativnog stresa izazvanog mehaničkom povredom biljke prilikom proizvodnje reznica, u bazalnom delu i listovima pet podloga za višnju i trešnju (Magriva 1 i 2, Gizela 5, Stepska (EGC) i “Oblačinska” višnja). Istovremeno, reznice su tretirane 0.5% rastvorima tri auksina (α-naftilsirćetna kiselina, NAA, indol- buterna kiselina, IBA i kombinacija ovih hormona, INCIT K), u periodu od 60 min, kako bi se utvrdio efekat auksina na antioksidantni status reznica višnje. Uzorci su uzimani nakon 0, 2, 4 i 6 dana. Kontrolu su činile reznice držane u vodi bez hormona. Intenzitet lipidne peroksidacije (LP) je meren kao produkcija malondialdehida (MDA), spektrofotometrijski na 532 nm, i izražen je u nmol MDA g-1 sveže mase.
Dobijeni rezultati su varirali u zavisnosti od podloge, organa biljke i primenjenih auksina. Utvrđeno je da je intenzitet LP u bazalnom delu bio najveći prvog dana, na- kon ozleđivanja, a da su svi ispitivani auksini snižavali produkciju MDA u narednim danima. Najbolji efekat pokazala je Magriva 1 tretirana sa NAA. U listovima, LP je bila najveća 2. dana, a najbolji efekat na smanjenje pokazali su primenjeni auksini u Stepskoj višnji (4. dana). Najveća produkcija MDA u listovima zabeležena je u Magrivoj 2 i Stepskoj višnji tretiranih sa IBA, 2. dana nakon odsecanja reznica (60-90% više od kontrole). Utvrđeno je da u većini podloga pod dejstvom ispitivanih hormona dolazi do smanjenja intenziteta LP što ukazuje na pozitivan efekat primenjenih auksina, ne samo na ožiljavanje reznica nego i na njihov antioksidantni sistem.",
publisher = "Novi Sad : University of Novi Sad, Faculty of Agriculture",
journal = "Contemporary Agriculture",
title = "Changes in the lipid peroxidation intensity in auxin treated cherry rootstocks softwood cuttings, Promene intenziteta lipidne peroksidacije u reznicama podloga za višnju i trešnju tretiranim auksinima",
pages = "229-221",
number = "3-4",
volume = "61",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2213"
}

Malenčić, Đ., Prvulović, D., Popović, M., Ognjanov, V., Ljubojević, M., Barać, G., Borković, B., Kevrešan, S.,& Kiprovski, B.. (2012). Changes in the lipid peroxidation intensity in auxin treated cherry rootstocks softwood cuttings. in Contemporary Agriculture
Novi Sad : University of Novi Sad, Faculty of Agriculture., 61(3-4), 221-229.
https://hdl.handle.net/21.15107/rcub_fiver_2213

Malenčić Đ, Prvulović D, Popović M, Ognjanov V, Ljubojević M, Barać G, Borković B, Kevrešan S, Kiprovski B. Changes in the lipid peroxidation intensity in auxin treated cherry rootstocks softwood cuttings. in Contemporary Agriculture. 2012;61(3-4):221-229.
https://hdl.handle.net/21.15107/rcub_fiver_2213 .

Malenčić, Đorđe, Prvulović, Dejan, Popović, Milan, Ognjanov, Vladislav, Ljubojević, Mirjana, Barać, Goran, Borković, Boško, Kevrešan, Slavko, Kiprovski, Biljana, "Changes in the lipid peroxidation intensity in auxin treated cherry rootstocks softwood cuttings" in Contemporary Agriculture, 61, no. 3-4 (2012):221-229,
https://hdl.handle.net/21.15107/rcub_fiver_2213 .