TY  - CONF
AU  - Pockovska, Marija
AU  - Glogovac, Svetlana
AU  - Kondić-Špika, Ankica
AU  - Trajkova, Fidanka
AU  - Koleva Gudeva, Ljiljana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3782
AB  - The anthers from six pepper genotypes, two hybrids Edita F1, Homera F1, and 4 cultivars: Duga
bela, Una, Amfora and Kurtovska kapija were used in two-year androgenesis experiment. The
anthers were isolated and cultivated on suitable nutrient media, following appropriate protocols.
All tested pepper genotypes responded differently in terms of callus and androgenic embryos
formation. Four androgenic regenerants (Edita_R1, Edita_R2, Edita_R3 and Edita_R4) from the
genotype Edita F1 were used for molecular evaluation and compared with the donor genotype.
The DNA was extracted according to the CTAB protocol, modified by Somma (2004). SSR markers
Hpms1-117, Hpms 1-168, Hpms 1-274, EPMS 650 and CAMS 117 were used for molecular
analysis. The molecular evaluation of the regenerants showed that all androgenic regenerants
had the same allele for all SSR loci as donor genotype Edita F1. Additionally, all androgenic
regenerants were homozygotes for the five tested loci.
PB  - Stip: Faculty of Agriculture, Goce Delcev University
C3  - Book of abstracts, 3rd International meeting agriscience & practice "ASP 2023", 19 - 20 April 2023, Stip, Republic of North Macedonia, 16
T1  - Molecular evaluation of four pepper androgenic regenerants
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3782
ER  - 

@conference{
author = "Pockovska, Marija and Glogovac, Svetlana and Kondić-Špika, Ankica and Trajkova, Fidanka and Koleva Gudeva, Ljiljana",
year = "2023",
abstract = "The anthers from six pepper genotypes, two hybrids Edita F1, Homera F1, and 4 cultivars: Duga
bela, Una, Amfora and Kurtovska kapija were used in two-year androgenesis experiment. The
anthers were isolated and cultivated on suitable nutrient media, following appropriate protocols.
All tested pepper genotypes responded differently in terms of callus and androgenic embryos
formation. Four androgenic regenerants (Edita_R1, Edita_R2, Edita_R3 and Edita_R4) from the
genotype Edita F1 were used for molecular evaluation and compared with the donor genotype.
The DNA was extracted according to the CTAB protocol, modified by Somma (2004). SSR markers
Hpms1-117, Hpms 1-168, Hpms 1-274, EPMS 650 and CAMS 117 were used for molecular
analysis. The molecular evaluation of the regenerants showed that all androgenic regenerants
had the same allele for all SSR loci as donor genotype Edita F1. Additionally, all androgenic
regenerants were homozygotes for the five tested loci.",
publisher = "Stip: Faculty of Agriculture, Goce Delcev University",
journal = "Book of abstracts, 3rd International meeting agriscience & practice "ASP 2023", 19 - 20 April 2023, Stip, Republic of North Macedonia, 16",
title = "Molecular evaluation of four pepper androgenic regenerants",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3782"
}

Pockovska, M., Glogovac, S., Kondić-Špika, A., Trajkova, F.,& Koleva Gudeva, L.. (2023). Molecular evaluation of four pepper androgenic regenerants. in Book of abstracts, 3rd International meeting agriscience & practice "ASP 2023", 19 - 20 April 2023, Stip, Republic of North Macedonia, 16
Stip: Faculty of Agriculture, Goce Delcev University..
https://hdl.handle.net/21.15107/rcub_fiver_3782

Pockovska M, Glogovac S, Kondić-Špika A, Trajkova F, Koleva Gudeva L. Molecular evaluation of four pepper androgenic regenerants. in Book of abstracts, 3rd International meeting agriscience & practice "ASP 2023", 19 - 20 April 2023, Stip, Republic of North Macedonia, 16. 2023;.
https://hdl.handle.net/21.15107/rcub_fiver_3782 .

Pockovska, Marija, Glogovac, Svetlana, Kondić-Špika, Ankica, Trajkova, Fidanka, Koleva Gudeva, Ljiljana, "Molecular evaluation of four pepper androgenic regenerants" in Book of abstracts, 3rd International meeting agriscience & practice "ASP 2023", 19 - 20 April 2023, Stip, Republic of North Macedonia, 16 (2023),
https://hdl.handle.net/21.15107/rcub_fiver_3782 .