TY  - JOUR
AU  - Kovačević, Branislav
AU  - Miladinović, Dragana
AU  - Orlović, Saša
AU  - Katanić, Marina
AU  - Kebert, Marko
AU  - Kovinčić, Jovana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1294
AB  - Background and Purpose: This paper analyses the lead tolerance and accumulation in white poplar genotypes in vitro, in order to optimize genotype evaluation and other procedures in their implementation in phytoremediation projects and landscaping in areas endangered by lead accumulation.
Material and Methods: The lead tolerance and accumulation of five white poplar genotypes after 35 days in vitro cultivation on media supplemented with lead was examined. The following Pb(NO3)2 concentrations were used: 0, 10-6, 10-5, 10-4 and 10-3 M. Tolerance analysis (described by tolerance indices) was based on morphological parameters, biomass accumulation and the content of photosynthetic pigments, while lead accumulation was described by shoot lead accumulation and shoot lead content.
Results and Conclusions: The chosen lead concentrations appeared not to be lethal. Moreover, the obtained results showed that the tested lead concentrations had a positive effect on: number of formed roots, shoot moisture content and shoot height. The best differentiation among the examined genotypes was gained by the tolerance index based on the shoot height on 10-4 M Pb(NO3)2. The shoot lead accumulation and shoot lead content significantly increased on 10-4 and 10-3 M Pb(NO3)2 media. Thus, the concentration of 10-4 M Pb(NO3)2 is recommended for further research. Two examined genotypes of horticultural value (LCM and LBM) achieved a significantly higher lead shoot content compared to the wide spread genotype “Villafranca” (almost 200% and 125% higher, respectively).
PB  - Croatian Forest Research Institute
T2  - South-East European Forestry
T1  - Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro
EP  - 12
IS  - 1
SP  - 3
VL  - 4
DO  - 10.15177/seefor.13-01
ER  - 

@article{
author = "Kovačević, Branislav and Miladinović, Dragana and Orlović, Saša and Katanić, Marina and Kebert, Marko and Kovinčić, Jovana",
year = "2013",
abstract = "Background and Purpose: This paper analyses the lead tolerance and accumulation in white poplar genotypes in vitro, in order to optimize genotype evaluation and other procedures in their implementation in phytoremediation projects and landscaping in areas endangered by lead accumulation.
Material and Methods: The lead tolerance and accumulation of five white poplar genotypes after 35 days in vitro cultivation on media supplemented with lead was examined. The following Pb(NO3)2 concentrations were used: 0, 10-6, 10-5, 10-4 and 10-3 M. Tolerance analysis (described by tolerance indices) was based on morphological parameters, biomass accumulation and the content of photosynthetic pigments, while lead accumulation was described by shoot lead accumulation and shoot lead content.
Results and Conclusions: The chosen lead concentrations appeared not to be lethal. Moreover, the obtained results showed that the tested lead concentrations had a positive effect on: number of formed roots, shoot moisture content and shoot height. The best differentiation among the examined genotypes was gained by the tolerance index based on the shoot height on 10-4 M Pb(NO3)2. The shoot lead accumulation and shoot lead content significantly increased on 10-4 and 10-3 M Pb(NO3)2 media. Thus, the concentration of 10-4 M Pb(NO3)2 is recommended for further research. Two examined genotypes of horticultural value (LCM and LBM) achieved a significantly higher lead shoot content compared to the wide spread genotype “Villafranca” (almost 200% and 125% higher, respectively).",
publisher = "Croatian Forest Research Institute",
journal = "South-East European Forestry",
title = "Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro",
pages = "12-3",
number = "1",
volume = "4",
doi = "10.15177/seefor.13-01"
}

Kovačević, B., Miladinović, D., Orlović, S., Katanić, M., Kebert, M.,& Kovinčić, J.. (2013). Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro. in South-East European Forestry
Croatian Forest Research Institute., 4(1), 3-12.
https://doi.org/10.15177/seefor.13-01

Kovačević B, Miladinović D, Orlović S, Katanić M, Kebert M, Kovinčić J. Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro. in South-East European Forestry. 2013;4(1):3-12.
doi:10.15177/seefor.13-01 .

Kovačević, Branislav, Miladinović, Dragana, Orlović, Saša, Katanić, Marina, Kebert, Marko, Kovinčić, Jovana, "Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro" in South-East European Forestry, 4, no. 1 (2013):3-12,
https://doi.org/10.15177/seefor.13-01 . .