Perović, Dragan

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orcid::0000-0002-0292-1693
  • Perović, Dragan (10)
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Author's Bibliography

SNP genotyping to explore genetic diversity: the case of Montenegrin durum wheat landraces

Velimirović, Ana; Jovović, Zoran; Perović, Dragan; Lehnert, Heinke; Mikić, Sanja; Pržulj, Novo; Mangini, Giacomo; Mandić, Dragan; Finetti-Sialer, Mariella Matilde

(The Italian Society of Agricultural Genetics, 2023)

TY  - CONF
AU  - Velimirović, Ana
AU  - Jovović, Zoran
AU  - Perović, Dragan
AU  - Lehnert, Heinke
AU  - Mikić, Sanja
AU  - Pržulj, Novo
AU  - Mangini, Giacomo
AU  - Mandić, Dragan
AU  - Finetti-Sialer, Mariella Matilde
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3866
AB  - Landraces of durum wheat (Triticum turgidum subsp. durum), were the main
cereals of Montenegro until the mid-20th century, where they were
collectively recognized as Rogosija. After the Second World War, the
introduction of high-yielding common wheat cultivars threatened the
survival of Rogosija landraces, that were almost extinct in farmers’
fields. Starting from 1955, sampling of durum wheat accessions in
Montenegrin regions allowed the conservation of a Rogosija durum collection
in the Montenegro Plant Gene Bank. This assortment represents an unexplored
durum wheat germplasm that can be analyzed for the identification of
valuable alleles, useful to increase the wheat crop adaptability to climate
change. Aims of this study were: i) to estimate the genetic diversity andpopulation structure of the Rogosija collection using SNP markers; ii) to
investigate correlation between genetic clusters and the Montenegro ecogeographic conditions. The collection was analyzed with a high-throughput
genotyping system based on the 25K Illumina SNP wheat array. A total of
6,915 high-quality SNPs were retained and mapped on the durum genome.
Principal components and phylogenetic analyses discriminated two different
genetic durum clusters. Analysis of molecular variance revealed that 16% of
the total variation was due to differences among the genetic clusters,
whereas the remaining variance occurred within clusters. To estimate
whether the genetic clusters detected are related to Montenegrin ecogeographic regions, the durum accessions were geo-referenced and evaluated
according to ecological data of the collecting sites. Interestingly, one
genetic cluster included samples located around Lake Skadar, while the
second genetic cluster comprised accession sampled in the Montenegrin
littoral coast. This result suggests that the Rogosija durum collection
stored in the Montenegro Plant Gene Bank enclosed two Rogosija durum
populations, evolved in two different eco-geographic micro-areas.
PB  - The Italian Society of Agricultural Genetics
C3  - Proceedings, 66th SIGA Annual Congress of The Italian Society of Agricultural Genetics, Bari, 5-8 September 2023
T1  - SNP genotyping to explore genetic diversity: the case of Montenegrin durum wheat landraces
SP  - 1.18
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3866
ER  - 
@conference{
author = "Velimirović, Ana and Jovović, Zoran and Perović, Dragan and Lehnert, Heinke and Mikić, Sanja and Pržulj, Novo and Mangini, Giacomo and Mandić, Dragan and Finetti-Sialer, Mariella Matilde",
year = "2023",
abstract = "Landraces of durum wheat (Triticum turgidum subsp. durum), were the main
cereals of Montenegro until the mid-20th century, where they were
collectively recognized as Rogosija. After the Second World War, the
introduction of high-yielding common wheat cultivars threatened the
survival of Rogosija landraces, that were almost extinct in farmers’
fields. Starting from 1955, sampling of durum wheat accessions in
Montenegrin regions allowed the conservation of a Rogosija durum collection
in the Montenegro Plant Gene Bank. This assortment represents an unexplored
durum wheat germplasm that can be analyzed for the identification of
valuable alleles, useful to increase the wheat crop adaptability to climate
change. Aims of this study were: i) to estimate the genetic diversity andpopulation structure of the Rogosija collection using SNP markers; ii) to
investigate correlation between genetic clusters and the Montenegro ecogeographic conditions. The collection was analyzed with a high-throughput
genotyping system based on the 25K Illumina SNP wheat array. A total of
6,915 high-quality SNPs were retained and mapped on the durum genome.
Principal components and phylogenetic analyses discriminated two different
genetic durum clusters. Analysis of molecular variance revealed that 16% of
the total variation was due to differences among the genetic clusters,
whereas the remaining variance occurred within clusters. To estimate
whether the genetic clusters detected are related to Montenegrin ecogeographic regions, the durum accessions were geo-referenced and evaluated
according to ecological data of the collecting sites. Interestingly, one
genetic cluster included samples located around Lake Skadar, while the
second genetic cluster comprised accession sampled in the Montenegrin
littoral coast. This result suggests that the Rogosija durum collection
stored in the Montenegro Plant Gene Bank enclosed two Rogosija durum
populations, evolved in two different eco-geographic micro-areas.",
publisher = "The Italian Society of Agricultural Genetics",
journal = "Proceedings, 66th SIGA Annual Congress of The Italian Society of Agricultural Genetics, Bari, 5-8 September 2023",
title = "SNP genotyping to explore genetic diversity: the case of Montenegrin durum wheat landraces",
pages = "1.18",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3866"
}
Velimirović, A., Jovović, Z., Perović, D., Lehnert, H., Mikić, S., Pržulj, N., Mangini, G., Mandić, D.,& Finetti-Sialer, M. M.. (2023). SNP genotyping to explore genetic diversity: the case of Montenegrin durum wheat landraces. in Proceedings, 66th SIGA Annual Congress of The Italian Society of Agricultural Genetics, Bari, 5-8 September 2023
The Italian Society of Agricultural Genetics., 1.18.
https://hdl.handle.net/21.15107/rcub_fiver_3866
Velimirović A, Jovović Z, Perović D, Lehnert H, Mikić S, Pržulj N, Mangini G, Mandić D, Finetti-Sialer MM. SNP genotyping to explore genetic diversity: the case of Montenegrin durum wheat landraces. in Proceedings, 66th SIGA Annual Congress of The Italian Society of Agricultural Genetics, Bari, 5-8 September 2023. 2023;:1.18.
https://hdl.handle.net/21.15107/rcub_fiver_3866 .
Velimirović, Ana, Jovović, Zoran, Perović, Dragan, Lehnert, Heinke, Mikić, Sanja, Pržulj, Novo, Mangini, Giacomo, Mandić, Dragan, Finetti-Sialer, Mariella Matilde, "SNP genotyping to explore genetic diversity: the case of Montenegrin durum wheat landraces" in Proceedings, 66th SIGA Annual Congress of The Italian Society of Agricultural Genetics, Bari, 5-8 September 2023 (2023):1.18,
https://hdl.handle.net/21.15107/rcub_fiver_3866 .

SNP Diversity and Genetic Structure of “Rogosija”, an Old Western Balkan Durum Wheat Collection

Velimirović, Ana; Jovović, Zoran; Perović, Dragan; Lehnert, Heike; Mikić, Sanja; Mandić, Dragan; Pržulj, Novo; Mangini, Giacomo; Finetti-Sialer, Mariella Matilde

(Basel : MDPI, 2023)

TY  - JOUR
AU  - Velimirović, Ana
AU  - Jovović, Zoran
AU  - Perović, Dragan
AU  - Lehnert, Heike
AU  - Mikić, Sanja
AU  - Mandić, Dragan
AU  - Pržulj, Novo
AU  - Mangini, Giacomo
AU  - Finetti-Sialer, Mariella Matilde
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4283
AB  - Durum wheat landraces represent a genetic resource for the identification and isolation of new valuable genes and alleles, useful to increase the crop adaptability to climate change. Several durum wheat landraces, all denominated “Rogosija”, were extensively cultivated in the Western Balkan Peninsula until the first half of the 20th century. Within the conservation program of the Montenegro Plant Gene Bank, these landraces were collected, but without being characterized. The main goal of this study was to estimate the genetic diversity of the “Rogosija collection” consisting of 89 durum accessions, using 17 morphological descriptors and the 25K Illumina single nucleotide polymorphism (SNP) array. The genetic structure analysis of the Rogosija collection showed two distinguished clusters localized in two different Montenegro eco-geographic micro-areas, characterized by continental Mediterranean climate and maritime Mediterranean climate. Data suggest that these clusters could be composed of two different Balkan durum landrace collections evolved in two different eco-geographic micro-areas. Moreover, the origin of Balkan durum landraces is discussed.
PB  - Basel : MDPI
T2  - Plants - Basel
T1  - SNP Diversity and Genetic Structure of “Rogosija”, an Old Western Balkan Durum Wheat Collection
IS  - 5
SP  - 1157
VL  - 12
DO  - 10.3390/plants12051157
ER  - 
@article{
author = "Velimirović, Ana and Jovović, Zoran and Perović, Dragan and Lehnert, Heike and Mikić, Sanja and Mandić, Dragan and Pržulj, Novo and Mangini, Giacomo and Finetti-Sialer, Mariella Matilde",
year = "2023",
abstract = "Durum wheat landraces represent a genetic resource for the identification and isolation of new valuable genes and alleles, useful to increase the crop adaptability to climate change. Several durum wheat landraces, all denominated “Rogosija”, were extensively cultivated in the Western Balkan Peninsula until the first half of the 20th century. Within the conservation program of the Montenegro Plant Gene Bank, these landraces were collected, but without being characterized. The main goal of this study was to estimate the genetic diversity of the “Rogosija collection” consisting of 89 durum accessions, using 17 morphological descriptors and the 25K Illumina single nucleotide polymorphism (SNP) array. The genetic structure analysis of the Rogosija collection showed two distinguished clusters localized in two different Montenegro eco-geographic micro-areas, characterized by continental Mediterranean climate and maritime Mediterranean climate. Data suggest that these clusters could be composed of two different Balkan durum landrace collections evolved in two different eco-geographic micro-areas. Moreover, the origin of Balkan durum landraces is discussed.",
publisher = "Basel : MDPI",
journal = "Plants - Basel",
title = "SNP Diversity and Genetic Structure of “Rogosija”, an Old Western Balkan Durum Wheat Collection",
number = "5",
pages = "1157",
volume = "12",
doi = "10.3390/plants12051157"
}
Velimirović, A., Jovović, Z., Perović, D., Lehnert, H., Mikić, S., Mandić, D., Pržulj, N., Mangini, G.,& Finetti-Sialer, M. M.. (2023). SNP Diversity and Genetic Structure of “Rogosija”, an Old Western Balkan Durum Wheat Collection. in Plants - Basel
Basel : MDPI., 12(5), 1157.
https://doi.org/10.3390/plants12051157
Velimirović A, Jovović Z, Perović D, Lehnert H, Mikić S, Mandić D, Pržulj N, Mangini G, Finetti-Sialer MM. SNP Diversity and Genetic Structure of “Rogosija”, an Old Western Balkan Durum Wheat Collection. in Plants - Basel. 2023;12(5):1157.
doi:10.3390/plants12051157 .
Velimirović, Ana, Jovović, Zoran, Perović, Dragan, Lehnert, Heike, Mikić, Sanja, Mandić, Dragan, Pržulj, Novo, Mangini, Giacomo, Finetti-Sialer, Mariella Matilde, "SNP Diversity and Genetic Structure of “Rogosija”, an Old Western Balkan Durum Wheat Collection" in Plants - Basel, 12, no. 5 (2023):1157,
https://doi.org/10.3390/plants12051157 . .
10

Morphological Characterisation of Monogenetic Traits of Tetraploid Wheat from Montenegro

Velimirović, Ana; Jovović, Zoran; Mikić, Sanja; Perović, Dragan; Pržulj, Novo

(Skopje : Ss Cyril and Methodius University in Skopje, Faculty of Agricultural Sciences and Food, 2022)

TY  - CONF
AU  - Velimirović, Ana
AU  - Jovović, Zoran
AU  - Mikić, Sanja
AU  - Perović, Dragan
AU  - Pržulj, Novo
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3762
AB  - Tetraploid wheat has been the most important cereal in Montenegro until the green revolution and
the introduction of elite common wheat cultivars. Realizing the risk of losing these populations,
at the Agricultural institute in Podgorica activities on the collection and conservation of these
populations were initiated. The entire population of tetraploid wheat of 89 samples is today
stored in the plant gene gene bank of Montenegro. In order to assess the diversity of these
populations, an assessment using UPOV morphological markers was performed in 2020. Five
monogenetic traits were included in the analysis: type of plant growth, time of ear emergence
(major genes controlling this trait are photoperiod sensitivity - ppd genes), ear glaucosity, plant
height (major genes controlling plant height are reduced height - rht genes), the length of
the ears without awns, the presence of awns and the seasonal type of plants (major genes
vernalization - vrn). Multiple correspondence analyses showed that there were no signicant 
dierences between these populations. Moreover, dimensions one and two explained only
9.6 and 7.8%, of the total variation, respectively, which indicates the common origin of the
population.
PB  - Skopje : Ss Cyril and Methodius University in Skopje, Faculty of Agricultural Sciences and Food
C3  - Book of Abstracts, 5th International Symposium for Agriculture and Food - ISAF 2022, 12-14 October 2022, Ohrid, North Macedonia. pp. 293.
T1  - Morphological Characterisation of Monogenetic Traits of Tetraploid Wheat from Montenegro
EP  - 293
SP  - 293
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3762
ER  - 
@conference{
author = "Velimirović, Ana and Jovović, Zoran and Mikić, Sanja and Perović, Dragan and Pržulj, Novo",
year = "2022",
abstract = "Tetraploid wheat has been the most important cereal in Montenegro until the green revolution and
the introduction of elite common wheat cultivars. Realizing the risk of losing these populations,
at the Agricultural institute in Podgorica activities on the collection and conservation of these
populations were initiated. The entire population of tetraploid wheat of 89 samples is today
stored in the plant gene gene bank of Montenegro. In order to assess the diversity of these
populations, an assessment using UPOV morphological markers was performed in 2020. Five
monogenetic traits were included in the analysis: type of plant growth, time of ear emergence
(major genes controlling this trait are photoperiod sensitivity - ppd genes), ear glaucosity, plant
height (major genes controlling plant height are reduced height - rht genes), the length of
the ears without awns, the presence of awns and the seasonal type of plants (major genes
vernalization - vrn). Multiple correspondence analyses showed that there were no signicant 
dierences between these populations. Moreover, dimensions one and two explained only
9.6 and 7.8%, of the total variation, respectively, which indicates the common origin of the
population.",
publisher = "Skopje : Ss Cyril and Methodius University in Skopje, Faculty of Agricultural Sciences and Food",
journal = "Book of Abstracts, 5th International Symposium for Agriculture and Food - ISAF 2022, 12-14 October 2022, Ohrid, North Macedonia. pp. 293.",
title = "Morphological Characterisation of Monogenetic Traits of Tetraploid Wheat from Montenegro",
pages = "293-293",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3762"
}
Velimirović, A., Jovović, Z., Mikić, S., Perović, D.,& Pržulj, N.. (2022). Morphological Characterisation of Monogenetic Traits of Tetraploid Wheat from Montenegro. in Book of Abstracts, 5th International Symposium for Agriculture and Food - ISAF 2022, 12-14 October 2022, Ohrid, North Macedonia. pp. 293.
Skopje : Ss Cyril and Methodius University in Skopje, Faculty of Agricultural Sciences and Food., 293-293.
https://hdl.handle.net/21.15107/rcub_fiver_3762
Velimirović A, Jovović Z, Mikić S, Perović D, Pržulj N. Morphological Characterisation of Monogenetic Traits of Tetraploid Wheat from Montenegro. in Book of Abstracts, 5th International Symposium for Agriculture and Food - ISAF 2022, 12-14 October 2022, Ohrid, North Macedonia. pp. 293.. 2022;:293-293.
https://hdl.handle.net/21.15107/rcub_fiver_3762 .
Velimirović, Ana, Jovović, Zoran, Mikić, Sanja, Perović, Dragan, Pržulj, Novo, "Morphological Characterisation of Monogenetic Traits of Tetraploid Wheat from Montenegro" in Book of Abstracts, 5th International Symposium for Agriculture and Food - ISAF 2022, 12-14 October 2022, Ohrid, North Macedonia. pp. 293. (2022):293-293,
https://hdl.handle.net/21.15107/rcub_fiver_3762 .

Response of kernel growth of barley genotypes with different row type to climatic factors before and after inflection point of grain filling

Dodig, Dejan; Kandić, Vesna; Zorić, Miroslav; Nikolić-Đorić, Emilija; Tančić-Živanov, Sonja; Perović, Dragan

(Elsevier, Amsterdam, 2020)

TY  - JOUR
AU  - Dodig, Dejan
AU  - Kandić, Vesna
AU  - Zorić, Miroslav
AU  - Nikolić-Đorić, Emilija
AU  - Tančić-Živanov, Sonja
AU  - Perović, Dragan
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/2048
AB  - Changes in patterns of rainfall and rising temperatures during grain development have already negatively affected yield gains of temperate cereals. This article reports on barley field trials with 15 two- and 10 six-row barley genotypes evaluated in eight environments where terminal drought was simulated by leaf defoliation applied seven days after heading. The experimental years were contrasting in terms of temperatures during grain filling. The focus of the study was to determine which barley type was more sensitive to terminal drought and high temperatures. The grain filling period was divided in two sub-periods: before (P1) and after (P2) inflection point (IP) of the growth curve, which occurs at the instant when the rate of accumulation ceases to accelerate and begins to slow down. For each period, climatic factors were calculated and their effects on the mean kernel growth rate (RG) were analyzed. To explore genotype x environment interactions for production per spike (PPS), the regression approach was adopted using climatic data in P1 and P2 as explanatory variables. Two-row barley had a significantly longer IP than six-row barley. IP was in a negative relationship with RG and PPS in both barley types. Six-row barley showed higher RG sensibility than two-row barley to drought stress during the period of the extensive kernel growth (P1). The number of days with moderately high (between 25 and 30 degrees C) and high (over 30 degrees C) temperatures had a higher negative effect on RG of two-row barley than six-row barley, particularly in P2. On the other hand, minimum temperatures were more negative for RG of the six-row barley than two-row barley, particularly under control conditions. In general, two-row barley showed better adaptation to low yielding environments, while six-row barley was more responsive to high yielding environments.
PB  - Elsevier, Amsterdam
T2  - Field Crops Research
T1  - Response of kernel growth of barley genotypes with different row type to climatic factors before and after inflection point of grain filling
VL  - 255
DO  - 10.1016/j.fcr.2020.107864
ER  - 
@article{
author = "Dodig, Dejan and Kandić, Vesna and Zorić, Miroslav and Nikolić-Đorić, Emilija and Tančić-Živanov, Sonja and Perović, Dragan",
year = "2020",
abstract = "Changes in patterns of rainfall and rising temperatures during grain development have already negatively affected yield gains of temperate cereals. This article reports on barley field trials with 15 two- and 10 six-row barley genotypes evaluated in eight environments where terminal drought was simulated by leaf defoliation applied seven days after heading. The experimental years were contrasting in terms of temperatures during grain filling. The focus of the study was to determine which barley type was more sensitive to terminal drought and high temperatures. The grain filling period was divided in two sub-periods: before (P1) and after (P2) inflection point (IP) of the growth curve, which occurs at the instant when the rate of accumulation ceases to accelerate and begins to slow down. For each period, climatic factors were calculated and their effects on the mean kernel growth rate (RG) were analyzed. To explore genotype x environment interactions for production per spike (PPS), the regression approach was adopted using climatic data in P1 and P2 as explanatory variables. Two-row barley had a significantly longer IP than six-row barley. IP was in a negative relationship with RG and PPS in both barley types. Six-row barley showed higher RG sensibility than two-row barley to drought stress during the period of the extensive kernel growth (P1). The number of days with moderately high (between 25 and 30 degrees C) and high (over 30 degrees C) temperatures had a higher negative effect on RG of two-row barley than six-row barley, particularly in P2. On the other hand, minimum temperatures were more negative for RG of the six-row barley than two-row barley, particularly under control conditions. In general, two-row barley showed better adaptation to low yielding environments, while six-row barley was more responsive to high yielding environments.",
publisher = "Elsevier, Amsterdam",
journal = "Field Crops Research",
title = "Response of kernel growth of barley genotypes with different row type to climatic factors before and after inflection point of grain filling",
volume = "255",
doi = "10.1016/j.fcr.2020.107864"
}
Dodig, D., Kandić, V., Zorić, M., Nikolić-Đorić, E., Tančić-Živanov, S.,& Perović, D.. (2020). Response of kernel growth of barley genotypes with different row type to climatic factors before and after inflection point of grain filling. in Field Crops Research
Elsevier, Amsterdam., 255.
https://doi.org/10.1016/j.fcr.2020.107864
Dodig D, Kandić V, Zorić M, Nikolić-Đorić E, Tančić-Živanov S, Perović D. Response of kernel growth of barley genotypes with different row type to climatic factors before and after inflection point of grain filling. in Field Crops Research. 2020;255.
doi:10.1016/j.fcr.2020.107864 .
Dodig, Dejan, Kandić, Vesna, Zorić, Miroslav, Nikolić-Đorić, Emilija, Tančić-Živanov, Sonja, Perović, Dragan, "Response of kernel growth of barley genotypes with different row type to climatic factors before and after inflection point of grain filling" in Field Crops Research, 255 (2020),
https://doi.org/10.1016/j.fcr.2020.107864 . .
4
2

Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm

Hladni, Nada; Zorić, Miroslav; Terzić, Sreten; Ćurčić, Nataša; Satović, Zlatko; Perović, Dragan; Panković, Dejana

(Springer, Dordrecht, 2018)

TY  - JOUR
AU  - Hladni, Nada
AU  - Zorić, Miroslav
AU  - Terzić, Sreten
AU  - Ćurčić, Nataša
AU  - Satović, Zlatko
AU  - Perović, Dragan
AU  - Panković, Dejana
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1830
AB  - Pre-breeding and elite breeding are two steps in creating high yielding sunflower hybrids that differ in well established procedures and selection methods. However, a methodology that bridge efficient use of introgression lines as product of pre-breeding procedures and their crossing to elite inbreed lines, is not yet very well established. Therefore, the development of cost- and time-efficient methods for the determination of best parent heterosis and the use of best inbred lines in crosses with introgression lines for obtaining high-yielding and stable hybrids is highly desirable. In this regard, sixteen Cytoplasmic Male Sterile (CMS) inbred lines (A) derived from four heterogeneous interspecific lines originating from three annual: H. debilis silvestris (DEB-SIL), H. praecox runyoni (PRA-RUN), H. deserticola (DES) and one perennial H. resinosus (RES) wild species were evaluated. Seven agronomic traits were measured over a period of 2 years and 38 DNA loci were analysed, in order to compare four different methods for the estimation of best parent heterosis (BPH). New inbred lines were characterized by Principal Component Analysis (PCA) of morphological traits and Principal Coordinate Analysis (PCoA) of molecular marker data. Line x tester mating design was used to evaluate General Combining Ability (GCA), while Genetic Distance (GC) estimated by markers was evaluated as a predictor of BPH by Locally Weighted Sequential Smoothing (LOESS). Analysis of combining ability is one of the most important tools breeders use to identify superior inbred lines on the basis of their performance in hybrid combinations. Results obtained in this research show that PCA of morphological and PCoA of molecular marker data on parental lines are generally in agreement with GCA effects for examined traits. GD versus BPH relationships indicate that intermediate to high GD between parental lines was optimal for best heterotic effects of most traits. In this study, we show that the combination of the PCA of morphological data, PCoA of molecular marker data and GD between parental lines is fast and affordable, giving the most important information for parental choice of introgression and elite lines in sunflower breeding programs.
PB  - Springer, Dordrecht
T2  - Euphytica
T1  - Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm
IS  - 7
VL  - 214
DO  - 10.1007/s10681-018-2197-0
ER  - 
@article{
author = "Hladni, Nada and Zorić, Miroslav and Terzić, Sreten and Ćurčić, Nataša and Satović, Zlatko and Perović, Dragan and Panković, Dejana",
year = "2018",
abstract = "Pre-breeding and elite breeding are two steps in creating high yielding sunflower hybrids that differ in well established procedures and selection methods. However, a methodology that bridge efficient use of introgression lines as product of pre-breeding procedures and their crossing to elite inbreed lines, is not yet very well established. Therefore, the development of cost- and time-efficient methods for the determination of best parent heterosis and the use of best inbred lines in crosses with introgression lines for obtaining high-yielding and stable hybrids is highly desirable. In this regard, sixteen Cytoplasmic Male Sterile (CMS) inbred lines (A) derived from four heterogeneous interspecific lines originating from three annual: H. debilis silvestris (DEB-SIL), H. praecox runyoni (PRA-RUN), H. deserticola (DES) and one perennial H. resinosus (RES) wild species were evaluated. Seven agronomic traits were measured over a period of 2 years and 38 DNA loci were analysed, in order to compare four different methods for the estimation of best parent heterosis (BPH). New inbred lines were characterized by Principal Component Analysis (PCA) of morphological traits and Principal Coordinate Analysis (PCoA) of molecular marker data. Line x tester mating design was used to evaluate General Combining Ability (GCA), while Genetic Distance (GC) estimated by markers was evaluated as a predictor of BPH by Locally Weighted Sequential Smoothing (LOESS). Analysis of combining ability is one of the most important tools breeders use to identify superior inbred lines on the basis of their performance in hybrid combinations. Results obtained in this research show that PCA of morphological and PCoA of molecular marker data on parental lines are generally in agreement with GCA effects for examined traits. GD versus BPH relationships indicate that intermediate to high GD between parental lines was optimal for best heterotic effects of most traits. In this study, we show that the combination of the PCA of morphological data, PCoA of molecular marker data and GD between parental lines is fast and affordable, giving the most important information for parental choice of introgression and elite lines in sunflower breeding programs.",
publisher = "Springer, Dordrecht",
journal = "Euphytica",
title = "Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm",
number = "7",
volume = "214",
doi = "10.1007/s10681-018-2197-0"
}
Hladni, N., Zorić, M., Terzić, S., Ćurčić, N., Satović, Z., Perović, D.,& Panković, D.. (2018). Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm. in Euphytica
Springer, Dordrecht., 214(7).
https://doi.org/10.1007/s10681-018-2197-0
Hladni N, Zorić M, Terzić S, Ćurčić N, Satović Z, Perović D, Panković D. Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm. in Euphytica. 2018;214(7).
doi:10.1007/s10681-018-2197-0 .
Hladni, Nada, Zorić, Miroslav, Terzić, Sreten, Ćurčić, Nataša, Satović, Zlatko, Perović, Dragan, Panković, Dejana, "Comparison of methods for the estimation of best parent heterosis among lines developed from interspecific sunflower germplasm" in Euphytica, 214, no. 7 (2018),
https://doi.org/10.1007/s10681-018-2197-0 . .
9
5
8

Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation

Dodig, Dejan; Kandić, Vesna; Zorić, Miroslav; Nikolić-Đorić, Emilija; Nikolić, Ana; Mutavdžić, Beba; Perović, Dragan; Šurlan-Momirović, Gordana

(CSIRO Publishing, Clayton, 2018)

TY  - JOUR
AU  - Dodig, Dejan
AU  - Kandić, Vesna
AU  - Zorić, Miroslav
AU  - Nikolić-Đorić, Emilija
AU  - Nikolić, Ana
AU  - Mutavdžić, Beba
AU  - Perović, Dragan
AU  - Šurlan-Momirović, Gordana
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1749
AB  - Barley (Hordeum vulgare L.) is often grown in sites with low rainfall and high temperature during grain filling. Because spike architecture is one of basic footprints of barley domestication, the importance of spikes in adaptation to different environments or abiotic stresses can be hypothesised. In order to compare different barley spike types in terms of kernel growth and yield components, we tested 15 two-row and 10 six-row winter genotypes in eight environments where terminal drought was simulated by defoliation at 7 days after heading (7 DAH). Control plants were grown intact. On average, two-row genotypes outyielded six-row genotypes by 17% under control conditions and 33% under simulated late drought. Observations of kernel dry weights from 7 DAH through to harvest maturity at 5-day intervals were regressed onto a measure of thermal time. After preliminary evaluation of four nonlinear (S-shaped) models for kernel dry-weight accumulation, the ordinary logistic model was deemed the most appropriate in most cases and was finally applied to all plant-growth curves. Four parameters were estimated from the logistic model. Whereas two earliness estimators (inflection point and thermal time needed to reach maximum kernel weight) were similar for the two barley types, maximum kernel weight (Y-max) and mean rate of kernel growth (RG) were higher (P lt 0.05) in two-row than in six-row barleys. Differences in Y-max and RG among six-row barley genotypes were greater between control and defoliation treatments than between years, whereas among two-row barley genotypes, differences between years were greater, suggesting better stability of six-row types and better drought tolerance of two-row types in the tested barley set.
PB  - CSIRO Publishing, Clayton
T2  - Crop & Pasture Science
T1  - Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation
EP  - 1224
IS  - 12
SP  - 1215
VL  - 69
DO  - 10.1071/CP18336
ER  - 
@article{
author = "Dodig, Dejan and Kandić, Vesna and Zorić, Miroslav and Nikolić-Đorić, Emilija and Nikolić, Ana and Mutavdžić, Beba and Perović, Dragan and Šurlan-Momirović, Gordana",
year = "2018",
abstract = "Barley (Hordeum vulgare L.) is often grown in sites with low rainfall and high temperature during grain filling. Because spike architecture is one of basic footprints of barley domestication, the importance of spikes in adaptation to different environments or abiotic stresses can be hypothesised. In order to compare different barley spike types in terms of kernel growth and yield components, we tested 15 two-row and 10 six-row winter genotypes in eight environments where terminal drought was simulated by defoliation at 7 days after heading (7 DAH). Control plants were grown intact. On average, two-row genotypes outyielded six-row genotypes by 17% under control conditions and 33% under simulated late drought. Observations of kernel dry weights from 7 DAH through to harvest maturity at 5-day intervals were regressed onto a measure of thermal time. After preliminary evaluation of four nonlinear (S-shaped) models for kernel dry-weight accumulation, the ordinary logistic model was deemed the most appropriate in most cases and was finally applied to all plant-growth curves. Four parameters were estimated from the logistic model. Whereas two earliness estimators (inflection point and thermal time needed to reach maximum kernel weight) were similar for the two barley types, maximum kernel weight (Y-max) and mean rate of kernel growth (RG) were higher (P lt 0.05) in two-row than in six-row barleys. Differences in Y-max and RG among six-row barley genotypes were greater between control and defoliation treatments than between years, whereas among two-row barley genotypes, differences between years were greater, suggesting better stability of six-row types and better drought tolerance of two-row types in the tested barley set.",
publisher = "CSIRO Publishing, Clayton",
journal = "Crop & Pasture Science",
title = "Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation",
pages = "1224-1215",
number = "12",
volume = "69",
doi = "10.1071/CP18336"
}
Dodig, D., Kandić, V., Zorić, M., Nikolić-Đorić, E., Nikolić, A., Mutavdžić, B., Perović, D.,& Šurlan-Momirović, G.. (2018). Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation. in Crop & Pasture Science
CSIRO Publishing, Clayton., 69(12), 1215-1224.
https://doi.org/10.1071/CP18336
Dodig D, Kandić V, Zorić M, Nikolić-Đorić E, Nikolić A, Mutavdžić B, Perović D, Šurlan-Momirović G. Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation. in Crop & Pasture Science. 2018;69(12):1215-1224.
doi:10.1071/CP18336 .
Dodig, Dejan, Kandić, Vesna, Zorić, Miroslav, Nikolić-Đorić, Emilija, Nikolić, Ana, Mutavdžić, Beba, Perović, Dragan, Šurlan-Momirović, Gordana, "Comparative kernel growth and yield components of two- and six-row barley (Hordeum vulgare) under terminal drought simulated by defoliation" in Crop & Pasture Science, 69, no. 12 (2018):1215-1224,
https://doi.org/10.1071/CP18336 . .
12
6
10

Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley

Miletić, Aleksandra; Panković, Dejana; Zorić, Miroslav; Pržulj, Novo; Šurlan-Momirović, Gordana; Jovanović, Marija; Radisavljević, Bojan; Perović, Dragan

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2018)

TY  - JOUR
AU  - Miletić, Aleksandra
AU  - Panković, Dejana
AU  - Zorić, Miroslav
AU  - Pržulj, Novo
AU  - Šurlan-Momirović, Gordana
AU  - Jovanović, Marija
AU  - Radisavljević, Bojan
AU  - Perović, Dragan
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1811
AB  - Domestication, selection and modern breeding narrow the genetic variability of barley, and thus create the need for re-integration of variability as a basis for barley breeding. In this study, 4 barley cultivars and 48 landraces from Serbian gene bank were analysed during two years. Phenotypic variability and genetic structure of the two quantitative spike traits: length and width, and one trait of grain: 1000 grain weight, were analyzed in a population with genotypes of known type of vegetation and spike form. Average spike length ranged from 4,2 to 10,2 cm, spike width 0,65 to 1,27 cm and 1000 grain weight from 27,58 to 51,35 g. Analysis of variance has a significant F value for all sources of variation in all traits. Analysis with 16 SSR markers detected a total of 105 allelic forms. Observing the groups of vegetation type and spike form, diversity of genes was the highest in the spring barley (two-row and six-row forms) and six-row barley (spring and winter types). The results of AMOVA test for spike form showed highly significant values for both sources of variation, while the intergroup component was dominant both for the type of vegetation (91,26 %) and form of the spike (90,83 %). Based on the cluster dendrogram 52 genotypes are classified into three main groups and 11 sub-groups. The results showed there is considerable variability of examined traits in the collection, so it can be used in barley breeding programs.
AB  - Domestikacija, selekcija i moderno oplemenjivanje suzili su genetičku varijabilnost ječma, a time stvorili potrebu za ponovnim stvaranjem varijabilnosti kao baze za oplemenjivanje ove biljne vrste. U radu je tokom dve godine ispitivano 4 sorte ječma i 48 lokalnih populacija iz Banke biljnih gena Srbije. Analizirana je fenotipska varijabilnost i genetička struktura dve kvantitativne osobine klasa: dužina i širina klasa, i jedna osobina zrna: masa 1000 zrna, u populaciji sa genotipovima poznatog tipa vegetacije i oblika klasa. Prosečna dužina klasa iznosila je 4,2-10,2 cm, širina 0,65-1,27 cm, a masa 1000 zrna 27,58-51,35 g. Analiza varijanse imala je značajnu F vrednost za sve izvore variranja kod svih osobina. Analizom šesnaest SSR markera detektovano je ukupno 105 alelnih formi. Posmatrajući grupe, tip vegetacije i oblik klasa, diverzitet gena je bio najveći kod jarog (dvoredog i šestoredog) i šestoredog (jarog i ozimog) ječma. Rezultati AMOVA testa za oblik klasa su pokazali visoko značajne vrednosti za oba izvora varijacije, dok je međugrupna komponenta bila dominantna i za tip vegetacije (91,26%) i za oblik klasa (90,83%). UPGMA klaster analizom konstruisan je dendogram kojim su 52 genotipa svrstana u tri glavne grupe i 11 podgrupa. Dobijeni rezultati su pokazali da u proučavanoj kolekciji postoji značajna varijabilnost ispitivanih osobina, koja se može koristiti u oplemenjivačkim programima ječma.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley
T1  - Fenotipska varijabilnost osobina klasa i genetička struktura populacije u kolekciji jarih ječmova
EP  - 58
IS  - 2
SP  - 49
VL  - 24
DO  - 10.5937/SelSem1802049M
ER  - 
@article{
author = "Miletić, Aleksandra and Panković, Dejana and Zorić, Miroslav and Pržulj, Novo and Šurlan-Momirović, Gordana and Jovanović, Marija and Radisavljević, Bojan and Perović, Dragan",
year = "2018",
abstract = "Domestication, selection and modern breeding narrow the genetic variability of barley, and thus create the need for re-integration of variability as a basis for barley breeding. In this study, 4 barley cultivars and 48 landraces from Serbian gene bank were analysed during two years. Phenotypic variability and genetic structure of the two quantitative spike traits: length and width, and one trait of grain: 1000 grain weight, were analyzed in a population with genotypes of known type of vegetation and spike form. Average spike length ranged from 4,2 to 10,2 cm, spike width 0,65 to 1,27 cm and 1000 grain weight from 27,58 to 51,35 g. Analysis of variance has a significant F value for all sources of variation in all traits. Analysis with 16 SSR markers detected a total of 105 allelic forms. Observing the groups of vegetation type and spike form, diversity of genes was the highest in the spring barley (two-row and six-row forms) and six-row barley (spring and winter types). The results of AMOVA test for spike form showed highly significant values for both sources of variation, while the intergroup component was dominant both for the type of vegetation (91,26 %) and form of the spike (90,83 %). Based on the cluster dendrogram 52 genotypes are classified into three main groups and 11 sub-groups. The results showed there is considerable variability of examined traits in the collection, so it can be used in barley breeding programs., Domestikacija, selekcija i moderno oplemenjivanje suzili su genetičku varijabilnost ječma, a time stvorili potrebu za ponovnim stvaranjem varijabilnosti kao baze za oplemenjivanje ove biljne vrste. U radu je tokom dve godine ispitivano 4 sorte ječma i 48 lokalnih populacija iz Banke biljnih gena Srbije. Analizirana je fenotipska varijabilnost i genetička struktura dve kvantitativne osobine klasa: dužina i širina klasa, i jedna osobina zrna: masa 1000 zrna, u populaciji sa genotipovima poznatog tipa vegetacije i oblika klasa. Prosečna dužina klasa iznosila je 4,2-10,2 cm, širina 0,65-1,27 cm, a masa 1000 zrna 27,58-51,35 g. Analiza varijanse imala je značajnu F vrednost za sve izvore variranja kod svih osobina. Analizom šesnaest SSR markera detektovano je ukupno 105 alelnih formi. Posmatrajući grupe, tip vegetacije i oblik klasa, diverzitet gena je bio najveći kod jarog (dvoredog i šestoredog) i šestoredog (jarog i ozimog) ječma. Rezultati AMOVA testa za oblik klasa su pokazali visoko značajne vrednosti za oba izvora varijacije, dok je međugrupna komponenta bila dominantna i za tip vegetacije (91,26%) i za oblik klasa (90,83%). UPGMA klaster analizom konstruisan je dendogram kojim su 52 genotipa svrstana u tri glavne grupe i 11 podgrupa. Dobijeni rezultati su pokazali da u proučavanoj kolekciji postoji značajna varijabilnost ispitivanih osobina, koja se može koristiti u oplemenjivačkim programima ječma.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley, Fenotipska varijabilnost osobina klasa i genetička struktura populacije u kolekciji jarih ječmova",
pages = "58-49",
number = "2",
volume = "24",
doi = "10.5937/SelSem1802049M"
}
Miletić, A., Panković, D., Zorić, M., Pržulj, N., Šurlan-Momirović, G., Jovanović, M., Radisavljević, B.,& Perović, D.. (2018). Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 24(2), 49-58.
https://doi.org/10.5937/SelSem1802049M
Miletić A, Panković D, Zorić M, Pržulj N, Šurlan-Momirović G, Jovanović M, Radisavljević B, Perović D. Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley. in Selekcija i semenarstvo. 2018;24(2):49-58.
doi:10.5937/SelSem1802049M .
Miletić, Aleksandra, Panković, Dejana, Zorić, Miroslav, Pržulj, Novo, Šurlan-Momirović, Gordana, Jovanović, Marija, Radisavljević, Bojan, Perović, Dragan, "Phenotypic variability of spike characteristics and genetic structure of population in the collection of spring barley" in Selekcija i semenarstvo, 24, no. 2 (2018):49-58,
https://doi.org/10.5937/SelSem1802049M . .
2

Molecular characterization of red clover genotypes utilizing microsatellite markers

Radinović, Irena; Vasiljević, Sanja; Branković, Gordana; Ahsyee, Salem R.; Momirović, Una; Perović, Dragan; Šurlan-Momirović, Gordana

(Inst Investigaciones Agropecuarias, Chillan, 2017)

TY  - JOUR
AU  - Radinović, Irena
AU  - Vasiljević, Sanja
AU  - Branković, Gordana
AU  - Ahsyee, Salem R.
AU  - Momirović, Una
AU  - Perović, Dragan
AU  - Šurlan-Momirović, Gordana
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1680
AB  - Genetic resources of red clover (Trifolium pratense L.) are the basis for the improvement of this important forage legume. The objective of this study was microsatellite characterization of the accessions from the collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. Molecular evaluation of 46 red clover genotypes was performed by applying the set of 14 primer pairs of microsatellite markers. These primer pairs amplified a total of 187 alleles, with an average of 13.36 alleles per locus and average polymorphism information content (PIC) value was 0.306. The minimum values of Dice genetic distances based on polymorphism of microsatellite markers were found among genotypes NCPGRU2 and NCPGRU5 (0.311) and the highest values of genetic distances were determined for a couple of genotypes Violeta and BGR2 (0.933). The average genetic distance between all pairs of genotypes amounted 0.587. The results of the principal coordinate analysis (PCoA) were consistent with the results obtained on the basis of cluster analysis, except that the PCoA allocated another four genotypes. There was no relationship between groups of genotypes formed by the use of cluster analyses and PCoA with their geographical origin. Analysis of molecular variance of 46 red clover genotypes by the status and ploidy level was significant, but it also suggested a weak genetic differentiation of groups formed on the basis of those characteristics. Observed groups of genotypes, according to the cluster analyses and PCoA of microsatellite data, could be used in future breeding programs for the selection of germplasm.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Molecular characterization of red clover genotypes utilizing microsatellite markers
EP  - 47
IS  - 1
SP  - 41
VL  - 77
DO  - 10.4067/S0718-58392017000100005
ER  - 
@article{
author = "Radinović, Irena and Vasiljević, Sanja and Branković, Gordana and Ahsyee, Salem R. and Momirović, Una and Perović, Dragan and Šurlan-Momirović, Gordana",
year = "2017",
abstract = "Genetic resources of red clover (Trifolium pratense L.) are the basis for the improvement of this important forage legume. The objective of this study was microsatellite characterization of the accessions from the collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. Molecular evaluation of 46 red clover genotypes was performed by applying the set of 14 primer pairs of microsatellite markers. These primer pairs amplified a total of 187 alleles, with an average of 13.36 alleles per locus and average polymorphism information content (PIC) value was 0.306. The minimum values of Dice genetic distances based on polymorphism of microsatellite markers were found among genotypes NCPGRU2 and NCPGRU5 (0.311) and the highest values of genetic distances were determined for a couple of genotypes Violeta and BGR2 (0.933). The average genetic distance between all pairs of genotypes amounted 0.587. The results of the principal coordinate analysis (PCoA) were consistent with the results obtained on the basis of cluster analysis, except that the PCoA allocated another four genotypes. There was no relationship between groups of genotypes formed by the use of cluster analyses and PCoA with their geographical origin. Analysis of molecular variance of 46 red clover genotypes by the status and ploidy level was significant, but it also suggested a weak genetic differentiation of groups formed on the basis of those characteristics. Observed groups of genotypes, according to the cluster analyses and PCoA of microsatellite data, could be used in future breeding programs for the selection of germplasm.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Molecular characterization of red clover genotypes utilizing microsatellite markers",
pages = "47-41",
number = "1",
volume = "77",
doi = "10.4067/S0718-58392017000100005"
}
Radinović, I., Vasiljević, S., Branković, G., Ahsyee, S. R., Momirović, U., Perović, D.,& Šurlan-Momirović, G.. (2017). Molecular characterization of red clover genotypes utilizing microsatellite markers. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 77(1), 41-47.
https://doi.org/10.4067/S0718-58392017000100005
Radinović I, Vasiljević S, Branković G, Ahsyee SR, Momirović U, Perović D, Šurlan-Momirović G. Molecular characterization of red clover genotypes utilizing microsatellite markers. in Chilean Journal of Agricultural Research. 2017;77(1):41-47.
doi:10.4067/S0718-58392017000100005 .
Radinović, Irena, Vasiljević, Sanja, Branković, Gordana, Ahsyee, Salem R., Momirović, Una, Perović, Dragan, Šurlan-Momirović, Gordana, "Molecular characterization of red clover genotypes utilizing microsatellite markers" in Chilean Journal of Agricultural Research, 77, no. 1 (2017):41-47,
https://doi.org/10.4067/S0718-58392017000100005 . .
9
3
10

Veritas temporis filia est: Truth is the daughter of time

Pržulj, Novo; Perović, Dragan; Mirosavljević, Milan; Nožinić, Miloš

(Društvo selekcionara i semenara Republike Srbije, Beograd, 2016)

TY  - JOUR
AU  - Pržulj, Novo
AU  - Perović, Dragan
AU  - Mirosavljević, Milan
AU  - Nožinić, Miloš
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1596
AB  - In 1865, Gregor Mendel presented the lectures 'Experiments in Plant Hybridization' concerning his results from cross-breeding experiments with different types of garden pea, performed in his monastery garden in Brno. Mendel studied easily observed pairs of opposite traits, such as purple or white flower, and discovered dominant and recessive traits. He concluded that parents pass separate and distinct factors (today called genes) on to their offspring that are responsible for inherited traits. However, the scientific community did not understand that; indeed it was the beginning of what becomes genetics. The lectures published in 1866, Mendel sent to more than 30 biologists across Europe, but almost no one commented them. In the next 35 years, these papers were only three times cited. The genetics became more important at the beginning of the 20th century, when three different research groups (Hugo de Vries, Carl Erich Correns and Erich von Tschermak with their co-workers) independently re-discovered Mendel's Laws of inheritance. However, as soon as the work was rediscovered, it created controversy. The closeness of Mendel's experimental observations to those predicted by his theories has led to numerous articles and ongoing debate about whether the data could have been obtained in the published form without some falsification. There have been many plausible arguments made for and against this view by a range of eminent geneticists and statisticians. Some have gone so far, as to suggest that the theories ensued from Mendel's two laws were not even correctly formulated in his original paper. The strongest supporters of Mendel's theory became biologist William Bateson and zoologist and geneticist Thomas Hunt Morgan. Morgan argued that genes are located on chromosomes and that the cells chromosomes hold the actual hereditary material, thus created what is now known as classical genetics. For his discovery concerning the role play by the chromosome in heredity, Morgan received the Nobel Prize in 1933. As the architect of genetic experimental and statistical analysis, Mendel remains the acknowledged father of genetics.
AB  - Rezultate ukrštanja različitih tipova vrtnog graška, koje je dobio u manastirskoj bašti u Brnu, Mendel je izneo 1865. godine u predavanju 'Eksperimenti u hibridizaciji biljaka'. Proučavajući osobine graška koje imaju lako prepozantljive dve alternative, kao što je npr. ljubičasta ili bela boja cveta, Mendel je otkrio dominantne i recesivne osobine. Zaključio je da roditelji prenose na svoje potomke posebne i različite faktore (koje danas nazivamo geni), koji su odgovorni za nasleđivanje osobina. Tadašnja naučna zajednica nije razumela da je to u stvari bio početak razvoja genetike. Predavanja su publikovana 1866. i Mendel ih je poslao na adresu više od 30 biologa širom Evrope, ali ih skoro niko nije komentarisao. U narednih 35 godina ovi radovi citirani su samo tri puta. Genetika je postala značajnija početkom 20. veka kada su istraživači iz tri različite istraživačke grupe (Hugo de Vries, Carl Erich Correns i Erich von Tschermak sa saradnicima), nezavisno jedan od drugih, ponovo otkrili Menedelove zakone. Međutim, odmah nakon ponovnog otkrića Mendelovih zakona došlo je do kontraverznih stavova. Mala odstupanja između eksperimentalnih i teorijskih frekvencija pokrenule su intenzivne debate o mogućnosti dobijanja takvih podataka, dok su neki izražavali sumnju da su podaci falsifikovani. Stavovi eminentnih statističara i genetičara u vezi Mendelove teorije kretali su se od njenog podržavanja do odbacivanja. Pojedinci su iznosili sumnju da teorije nisu pravilno postavljene ni u originalnom radu. Biolog Wiliam Bateson i zoolog i genetičar Thomas Hunt Morgan bili su najveće pristalice Mendelove teorije. Morgan je tvrdio da se geni nalaze na hromozomima, odnosno da hromozomi sadrže nasledni materijal, što je u suštini značilo promovisanje klasične genetike, za što je Morgan dobio Nobelovu nagradu. Kao osnivač genetičkih eksperimenata i statističke analize Mendel se smatra ocem genetike.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Veritas temporis filia est: Truth is the daughter of time
T1  - Istina je kći vremena
EP  - 62
IS  - 1
SP  - 53
VL  - 22
DO  - 10.5937/SelSem1601053P
ER  - 
@article{
author = "Pržulj, Novo and Perović, Dragan and Mirosavljević, Milan and Nožinić, Miloš",
year = "2016",
abstract = "In 1865, Gregor Mendel presented the lectures 'Experiments in Plant Hybridization' concerning his results from cross-breeding experiments with different types of garden pea, performed in his monastery garden in Brno. Mendel studied easily observed pairs of opposite traits, such as purple or white flower, and discovered dominant and recessive traits. He concluded that parents pass separate and distinct factors (today called genes) on to their offspring that are responsible for inherited traits. However, the scientific community did not understand that; indeed it was the beginning of what becomes genetics. The lectures published in 1866, Mendel sent to more than 30 biologists across Europe, but almost no one commented them. In the next 35 years, these papers were only three times cited. The genetics became more important at the beginning of the 20th century, when three different research groups (Hugo de Vries, Carl Erich Correns and Erich von Tschermak with their co-workers) independently re-discovered Mendel's Laws of inheritance. However, as soon as the work was rediscovered, it created controversy. The closeness of Mendel's experimental observations to those predicted by his theories has led to numerous articles and ongoing debate about whether the data could have been obtained in the published form without some falsification. There have been many plausible arguments made for and against this view by a range of eminent geneticists and statisticians. Some have gone so far, as to suggest that the theories ensued from Mendel's two laws were not even correctly formulated in his original paper. The strongest supporters of Mendel's theory became biologist William Bateson and zoologist and geneticist Thomas Hunt Morgan. Morgan argued that genes are located on chromosomes and that the cells chromosomes hold the actual hereditary material, thus created what is now known as classical genetics. For his discovery concerning the role play by the chromosome in heredity, Morgan received the Nobel Prize in 1933. As the architect of genetic experimental and statistical analysis, Mendel remains the acknowledged father of genetics., Rezultate ukrštanja različitih tipova vrtnog graška, koje je dobio u manastirskoj bašti u Brnu, Mendel je izneo 1865. godine u predavanju 'Eksperimenti u hibridizaciji biljaka'. Proučavajući osobine graška koje imaju lako prepozantljive dve alternative, kao što je npr. ljubičasta ili bela boja cveta, Mendel je otkrio dominantne i recesivne osobine. Zaključio je da roditelji prenose na svoje potomke posebne i različite faktore (koje danas nazivamo geni), koji su odgovorni za nasleđivanje osobina. Tadašnja naučna zajednica nije razumela da je to u stvari bio početak razvoja genetike. Predavanja su publikovana 1866. i Mendel ih je poslao na adresu više od 30 biologa širom Evrope, ali ih skoro niko nije komentarisao. U narednih 35 godina ovi radovi citirani su samo tri puta. Genetika je postala značajnija početkom 20. veka kada su istraživači iz tri različite istraživačke grupe (Hugo de Vries, Carl Erich Correns i Erich von Tschermak sa saradnicima), nezavisno jedan od drugih, ponovo otkrili Menedelove zakone. Međutim, odmah nakon ponovnog otkrića Mendelovih zakona došlo je do kontraverznih stavova. Mala odstupanja između eksperimentalnih i teorijskih frekvencija pokrenule su intenzivne debate o mogućnosti dobijanja takvih podataka, dok su neki izražavali sumnju da su podaci falsifikovani. Stavovi eminentnih statističara i genetičara u vezi Mendelove teorije kretali su se od njenog podržavanja do odbacivanja. Pojedinci su iznosili sumnju da teorije nisu pravilno postavljene ni u originalnom radu. Biolog Wiliam Bateson i zoolog i genetičar Thomas Hunt Morgan bili su najveće pristalice Mendelove teorije. Morgan je tvrdio da se geni nalaze na hromozomima, odnosno da hromozomi sadrže nasledni materijal, što je u suštini značilo promovisanje klasične genetike, za što je Morgan dobio Nobelovu nagradu. Kao osnivač genetičkih eksperimenata i statističke analize Mendel se smatra ocem genetike.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Veritas temporis filia est: Truth is the daughter of time, Istina je kći vremena",
pages = "62-53",
number = "1",
volume = "22",
doi = "10.5937/SelSem1601053P"
}
Pržulj, N., Perović, D., Mirosavljević, M.,& Nožinić, M.. (2016). Veritas temporis filia est: Truth is the daughter of time. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 22(1), 53-62.
https://doi.org/10.5937/SelSem1601053P
Pržulj N, Perović D, Mirosavljević M, Nožinić M. Veritas temporis filia est: Truth is the daughter of time. in Selekcija i semenarstvo. 2016;22(1):53-62.
doi:10.5937/SelSem1601053P .
Pržulj, Novo, Perović, Dragan, Mirosavljević, Milan, Nožinić, Miloš, "Veritas temporis filia est: Truth is the daughter of time" in Selekcija i semenarstvo, 22, no. 1 (2016):53-62,
https://doi.org/10.5937/SelSem1601053P . .
1

Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting

Šurlan-Momirović, Gordana; Krämer, Ilona; Bratković, Kamenko; Zorić, Miroslav; Momirović, Una; Branković, Gordana; Ćalić, Irena; Kandić, Vesna; Pržulj, Novo; Ordon, Frank; Perović, Dragan

(Društvo genetičara Srbije, Beograd, 2013)

TY  - JOUR
AU  - Šurlan-Momirović, Gordana
AU  - Krämer, Ilona
AU  - Bratković, Kamenko
AU  - Zorić, Miroslav
AU  - Momirović, Una
AU  - Branković, Gordana
AU  - Ćalić, Irena
AU  - Kandić, Vesna
AU  - Pržulj, Novo
AU  - Ordon, Frank
AU  - Perović, Dragan
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1215
AB  - Molecular diversity of 145 barley (Hordeum vulgare subsp. vulgare L.) accessions from the Serbian GenBank was assessed by single sequence repeats (SSR) markers. A set of 15 SSRs, covering all chromosomes of the diploid barley genome with 2-3 SSR markers per chromosome, with a range of 4-18 alleles per locus were used. In total, 15 loci and 119 alleles were detected, with an average of 7.93 alleles per locus. The Polymorphic information content value ranged from 0.220 to 0.782 with a mean value of 0.534. Regarding the growth habit and row type groups, gene diversity was comparatively higher for the spring (0.616) and six-rowed accessions (0.616) than for the winter and two- rowed accessions (0.322 and 0.478, respectively). Analysis of molecular variance showed that all sources of variation were significant (P  lt  0.01), but the between-group component was predominant (76.85%) for growth habit and 89.45% for row type. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on the shared allele distance (DSA) matrix estimated on the SSR data assigned the genotypes into two clusters - the first smaller consisting of the six 6-rowed spring cultivars and the second comprising six subclusters. Genotype MBR1012 was separated from all other genotypes that constitute UPGMA tree. The associations of genotypes belonging to different growth habit and row type groups were assessed using Principal Coordinate Analysis revealing separation of winter growth habit group from facultative one. The use of the STRUCTURE clustering algorithm allowed the identification of 2 subpopulations of genotypes.
AB  - Molekularni diverzitet 145 genotipova ječma (Hordeum vulgare subsp. vulgare L.) iz Gen Banke Srbije je procenjen SSR markerima. Korišćeno je SSR markera sa svih hromozoma diploidnog genoma ječma u proseku 2-3 markera po hromozomu i sa 4-18 alela po lokusu. Utvrđeno je 15 lokusa i 119 alela sa prosekom od 7.93 alela po lokusu. PIC je bio u opsegu od 0.220 do 0.782 sa prosekom 0.534. Genski diverzitet je bio veći za jare (0.616) i šestorede genotipove (0.616) u odnosu na ozime i dvorede (0.322 i 0.478). Analizom molekularne varijanse utvrđena je značajnost svih izvora variranja (P  lt  0.01), ali je međugrupna komponenta dominirala. Primenom metoda UPGMA analize zasnovane na zajedničkoj distanci alela (DSA) na osnovu SSR podataka dobijeno je grupisanje genotipove u dva klastera-jedan manji koji sadrži šest šestoredih jarih genotipova ječma i drugi koji je obuhvatao šest podklastera. Genotip MBR1012 je bio odvojen od svih ostalih genotipova. Korišćenjem metoda Osnovna Analiza Koordinata dobijeno je razdvajanje ozimih genotipova ječma od fakultativnih. Utvrđeno je postojanje dve podpopulacije genotipova primenom algoritma grupisanja STRUCTURE.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting
T1  - Molekularna karakterizacija genotipova ječma (Hordeum vulgare L.) iz gen banke Srbije SSR markerima
EP  - 180
IS  - 1
SP  - 167
VL  - 45
DO  - 10.2298/GENSR1301167S
ER  - 
@article{
author = "Šurlan-Momirović, Gordana and Krämer, Ilona and Bratković, Kamenko and Zorić, Miroslav and Momirović, Una and Branković, Gordana and Ćalić, Irena and Kandić, Vesna and Pržulj, Novo and Ordon, Frank and Perović, Dragan",
year = "2013",
abstract = "Molecular diversity of 145 barley (Hordeum vulgare subsp. vulgare L.) accessions from the Serbian GenBank was assessed by single sequence repeats (SSR) markers. A set of 15 SSRs, covering all chromosomes of the diploid barley genome with 2-3 SSR markers per chromosome, with a range of 4-18 alleles per locus were used. In total, 15 loci and 119 alleles were detected, with an average of 7.93 alleles per locus. The Polymorphic information content value ranged from 0.220 to 0.782 with a mean value of 0.534. Regarding the growth habit and row type groups, gene diversity was comparatively higher for the spring (0.616) and six-rowed accessions (0.616) than for the winter and two- rowed accessions (0.322 and 0.478, respectively). Analysis of molecular variance showed that all sources of variation were significant (P  lt  0.01), but the between-group component was predominant (76.85%) for growth habit and 89.45% for row type. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on the shared allele distance (DSA) matrix estimated on the SSR data assigned the genotypes into two clusters - the first smaller consisting of the six 6-rowed spring cultivars and the second comprising six subclusters. Genotype MBR1012 was separated from all other genotypes that constitute UPGMA tree. The associations of genotypes belonging to different growth habit and row type groups were assessed using Principal Coordinate Analysis revealing separation of winter growth habit group from facultative one. The use of the STRUCTURE clustering algorithm allowed the identification of 2 subpopulations of genotypes., Molekularni diverzitet 145 genotipova ječma (Hordeum vulgare subsp. vulgare L.) iz Gen Banke Srbije je procenjen SSR markerima. Korišćeno je SSR markera sa svih hromozoma diploidnog genoma ječma u proseku 2-3 markera po hromozomu i sa 4-18 alela po lokusu. Utvrđeno je 15 lokusa i 119 alela sa prosekom od 7.93 alela po lokusu. PIC je bio u opsegu od 0.220 do 0.782 sa prosekom 0.534. Genski diverzitet je bio veći za jare (0.616) i šestorede genotipove (0.616) u odnosu na ozime i dvorede (0.322 i 0.478). Analizom molekularne varijanse utvrđena je značajnost svih izvora variranja (P  lt  0.01), ali je međugrupna komponenta dominirala. Primenom metoda UPGMA analize zasnovane na zajedničkoj distanci alela (DSA) na osnovu SSR podataka dobijeno je grupisanje genotipove u dva klastera-jedan manji koji sadrži šest šestoredih jarih genotipova ječma i drugi koji je obuhvatao šest podklastera. Genotip MBR1012 je bio odvojen od svih ostalih genotipova. Korišćenjem metoda Osnovna Analiza Koordinata dobijeno je razdvajanje ozimih genotipova ječma od fakultativnih. Utvrđeno je postojanje dve podpopulacije genotipova primenom algoritma grupisanja STRUCTURE.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting, Molekularna karakterizacija genotipova ječma (Hordeum vulgare L.) iz gen banke Srbije SSR markerima",
pages = "180-167",
number = "1",
volume = "45",
doi = "10.2298/GENSR1301167S"
}
Šurlan-Momirović, G., Krämer, I., Bratković, K., Zorić, M., Momirović, U., Branković, G., Ćalić, I., Kandić, V., Pržulj, N., Ordon, F.,& Perović, D.. (2013). Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 45(1), 167-180.
https://doi.org/10.2298/GENSR1301167S
Šurlan-Momirović G, Krämer I, Bratković K, Zorić M, Momirović U, Branković G, Ćalić I, Kandić V, Pržulj N, Ordon F, Perović D. Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting. in Genetika-Belgrade. 2013;45(1):167-180.
doi:10.2298/GENSR1301167S .
Šurlan-Momirović, Gordana, Krämer, Ilona, Bratković, Kamenko, Zorić, Miroslav, Momirović, Una, Branković, Gordana, Ćalić, Irena, Kandić, Vesna, Pržulj, Novo, Ordon, Frank, Perović, Dragan, "Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting" in Genetika-Belgrade, 45, no. 1 (2013):167-180,
https://doi.org/10.2298/GENSR1301167S . .
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