@conference{
author = "Velimirović, Ana and Jovović, Zoran and Perović, Dragan and Lehnert, Heinke and Mikić, Sanja and Pržulj, Novo and Mangini, Giacomo and Mandić, Dragan and Finetti-Sialer, Mariella Matilde",
year = "2023",
abstract = "Landraces of durum wheat (Triticum turgidum subsp. durum), were the main
cereals of Montenegro until the mid-20th century, where they were
collectively recognized as Rogosija. After the Second World War, the
introduction of high-yielding common wheat cultivars threatened the
survival of Rogosija landraces, that were almost extinct in farmers’
fields. Starting from 1955, sampling of durum wheat accessions in
Montenegrin regions allowed the conservation of a Rogosija durum collection
in the Montenegro Plant Gene Bank. This assortment represents an unexplored
durum wheat germplasm that can be analyzed for the identification of
valuable alleles, useful to increase the wheat crop adaptability to climate
change. Aims of this study were: i) to estimate the genetic diversity andpopulation structure of the Rogosija collection using SNP markers; ii) to
investigate correlation between genetic clusters and the Montenegro ecogeographic conditions. The collection was analyzed with a high-throughput
genotyping system based on the 25K Illumina SNP wheat array. A total of
6,915 high-quality SNPs were retained and mapped on the durum genome.
Principal components and phylogenetic analyses discriminated two different
genetic durum clusters. Analysis of molecular variance revealed that 16% of
the total variation was due to differences among the genetic clusters,
whereas the remaining variance occurred within clusters. To estimate
whether the genetic clusters detected are related to Montenegrin ecogeographic regions, the durum accessions were geo-referenced and evaluated
according to ecological data of the collecting sites. Interestingly, one
genetic cluster included samples located around Lake Skadar, while the
second genetic cluster comprised accession sampled in the Montenegrin
littoral coast. This result suggests that the Rogosija durum collection
stored in the Montenegro Plant Gene Bank enclosed two Rogosija durum
populations, evolved in two different eco-geographic micro-areas.",
publisher = "The Italian Society of Agricultural Genetics",
journal = "Proceedings, 66th SIGA Annual Congress of The Italian Society of Agricultural Genetics, Bari, 5-8 September 2023",
title = "SNP genotyping to explore genetic diversity: the case of Montenegrin durum wheat landraces",
pages = "1.18",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3866"
}
