@article{
author = "Bursać, Mira and Atanacković, Milica and Cvejić, Jelena and Vasiljević, Sanja",
year = "2011",
abstract = "Introduction: Red clover (Trifolium pratense L., Fabaceae) is one of the main sources of phytoestrogens, among which are the most present isoflavones: daidzein, genistein, formononetin and biochanin A. These substances are used for reduction of menopausal symptoms, and they are considered to have certain role in prevention of osteoporosis, cancer and cardiovascular diseases. Aim: The aim of this study was to determine content of isoflavones in different red clover cultivars, cultivated in Vojvodina, and also in their plant parts. Material and methods: Five cultivars were analyzed (stem, leaf and flower). After extraction, isoflavones were identified and quantified in samples by high-performance liquid chromatography (HPLC). Results: Total isoflavone content varied between 0,41 do 3,81 mg/g, and it was on average the highest in leaves (2,73±0,97 mg/g), and the lowest in stems of red clover cultivars (0,47±0,06 mg/g). In leaves formononetin was dominant (on average 1,62±0,97 mg/g), while the lowest average content had biochanin A in stems (0,04±0,06 mg/g). Cultivar m21 had the highest average total isoflavone content in stem, leaf and flower - 1,78±1,77 mg/g. Conclusion: The highest concentration of isoflavones was determined in leaves. Cultivar m21 was the richest in isoflavones, and on average in all analyzed samples formononetin was the most present isoflavone. ., Uvod: Crvena detelina (Trifolium pratense L., Fabaceae) je jedan od značajnijih izvora fitoestrogena, od kojih su najzastupljeniji izoflavoni: daidzein, genistein, formononetin i biohanin A. Ove supstance se koriste za ublažavanje tegoba u menopauzi, a smatra se da mogu da imaju ulogu u prevenciji osteoporoze, kancera i kardioloških poremećaja. Cilj: Cilj rad je utvrđivanje sadržaja pojedinačnih izoflavona različitih sorti crvene deteline gajenih na području Vojvodine, kao i u pojedinim delovima biljke. Materijal i metode: Analizirano je pet sorti crvene deteline (stabljika, list i cvet). Nakon ekstrakcije, u uzorcima su identifikovani i kvantifikovani izoflavoni visoko-efikasnom tečnom hromatogafijom (HPLC). Rezultati: Sadržaj ukupnih izoflavona varira od 0,41 do 3,81 mg/g, a u proseku najzastupljeniji su u listovima (2,73±0,97 mg/g), dok stabljike crvene deteline sadrže najmanju koncentraciju tih jedinjenja (0,47±0,06 mg/g). U listovima je dominantan formononetin (prosečno 1,62±0,97 mg/g), dok je najmanji prosečni sadržaj biohanina A u stabljikama ispitivanih uzoraka (0,04±0,06 mg/g). U sorti m21 prosečna vrednost ukupnih izoflavona u stablu, listu i cvetu je najveća i iznosi 1,78±1,77 mg/g. Zaključak: Najveća koncentracija izoflavona utvrđena je u listovima. Sorta m21 je najbogatija izoflavonima, a u svim analiziranim uzorcima, u proseku je najviše zastupljen formononetin. .",
publisher = "Nestranačko, nevladino i neprofitno udruženje građana Medicina danas - pokret evropskih inicijativa, Novi Sad",
journal = "Medicina danas",
title = "Analysis of phytoestrogens in red clover, Analiza fitoestrogena crvene deteline",
pages = "265-259",
number = "7-9",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_fiver_991"
}
