TY  - JOUR
AU  - Kondić-Špika, Ankica
AU  - Glogovac, Svetlana
AU  - Trkulja, Dragana
AU  - Marjanović-Jeromela, Ana
AU  - Marjanović, Milica
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3112
AB  - The objective of this study was to evaluate boron tolerance of wheat genotypes using mature embryo culture. The analysis involved 79 recombinant inbred lines of the International Triticeae Mapping Initiative (ITMI) population and three Serbian varieties with known boron (B) tolerance (Pobeda – sensitive S, Balerina -medium tolerant MT, and Nevesinjka – tolerant T). The evaluation was performed on a modified MS medium to which 15 mM of boric acid was added. The control medium contained no excess B. Callus fresh weight (CFW) and reduction of fresh callus weight (RFCW) were determined after one month of cultivation. ANOVA has shown highly significant effect of genotype, the media, and their interaction to callus tissue growth and also significant genotypic effect on RFCW. Majority of genotypes (39) had sensitive reaction to excess boron, twenty-three were medium tolerant, while four of them were tolerant. The obtained results can be potentially used for mapping QTLs associated with tolerance to excess B in wheat breeding program.
AB  - Cilj ovog istraživanja bio je da se proceni tolerantnost genotipova pšenice na suvišak bora primenom kulture zrelog embriona. Analizom je obuhvaćeno 79 rekombinantnih inbred linija ITMI mapirajuće populacije i 3 sorte poznate tolerantnosti na bor (Pobeda – osetljiva, Balerina – srednje tolerantna i Nevesinjka – tolerantna) poreklom iz Srbije. Procena tolerantnosti je rađena na modifikovanoj MS hranljivoj podlozi u koju je dodato 15 mM borne kiseline. Kontrolna podloga nije sadržala suvišak bora. Sveža masa kalusa i redukcija sveže mase kalusa, određene su nakon mesec dana gajenja. ANOVA je pokazala veoma značajan efekat genotipa, hranljive podloge i njihove interakcije na rast kalusa, kao i značajan genotipski efekat na redukciju sveže mase kalusa. Većina genotipova (39) je imala osetljivu reakciju na suvišak bora, 23 su bila srednje tolerantna dok su 4 genotipa bila tolerantna. Dobijeni rezultati se potencijalno mogu koristiti za mapiranje gena i QTL-ova povezanih sa tolerancijom na suvišak B u oplemenjivanju pšenice.
PB  - Beograd : Društvo genetičara Srbije
T2  - Genetika-Belgrade
T1  - Response of wheat genotypes to excess boron estimated by in vitro culture
T1  - Reakcija genotipova pšenice na suvišak bora u kulturi in vitro
EP  - 919
IS  - 2
SP  - 907
VL  - 54
DO  - 10.2298/GENSR2202000K
ER  - 

@article{
author = "Kondić-Špika, Ankica and Glogovac, Svetlana and Trkulja, Dragana and Marjanović-Jeromela, Ana and Marjanović, Milica",
year = "2022",
abstract = "The objective of this study was to evaluate boron tolerance of wheat genotypes using mature embryo culture. The analysis involved 79 recombinant inbred lines of the International Triticeae Mapping Initiative (ITMI) population and three Serbian varieties with known boron (B) tolerance (Pobeda – sensitive S, Balerina -medium tolerant MT, and Nevesinjka – tolerant T). The evaluation was performed on a modified MS medium to which 15 mM of boric acid was added. The control medium contained no excess B. Callus fresh weight (CFW) and reduction of fresh callus weight (RFCW) were determined after one month of cultivation. ANOVA has shown highly significant effect of genotype, the media, and their interaction to callus tissue growth and also significant genotypic effect on RFCW. Majority of genotypes (39) had sensitive reaction to excess boron, twenty-three were medium tolerant, while four of them were tolerant. The obtained results can be potentially used for mapping QTLs associated with tolerance to excess B in wheat breeding program., Cilj ovog istraživanja bio je da se proceni tolerantnost genotipova pšenice na suvišak bora primenom kulture zrelog embriona. Analizom je obuhvaćeno 79 rekombinantnih inbred linija ITMI mapirajuće populacije i 3 sorte poznate tolerantnosti na bor (Pobeda – osetljiva, Balerina – srednje tolerantna i Nevesinjka – tolerantna) poreklom iz Srbije. Procena tolerantnosti je rađena na modifikovanoj MS hranljivoj podlozi u koju je dodato 15 mM borne kiseline. Kontrolna podloga nije sadržala suvišak bora. Sveža masa kalusa i redukcija sveže mase kalusa, određene su nakon mesec dana gajenja. ANOVA je pokazala veoma značajan efekat genotipa, hranljive podloge i njihove interakcije na rast kalusa, kao i značajan genotipski efekat na redukciju sveže mase kalusa. Većina genotipova (39) je imala osetljivu reakciju na suvišak bora, 23 su bila srednje tolerantna dok su 4 genotipa bila tolerantna. Dobijeni rezultati se potencijalno mogu koristiti za mapiranje gena i QTL-ova povezanih sa tolerancijom na suvišak B u oplemenjivanju pšenice.",
publisher = "Beograd : Društvo genetičara Srbije",
journal = "Genetika-Belgrade",
title = "Response of wheat genotypes to excess boron estimated by in vitro culture, Reakcija genotipova pšenice na suvišak bora u kulturi in vitro",
pages = "919-907",
number = "2",
volume = "54",
doi = "10.2298/GENSR2202000K"
}

Kondić-Špika, A., Glogovac, S., Trkulja, D., Marjanović-Jeromela, A.,& Marjanović, M.. (2022). Response of wheat genotypes to excess boron estimated by in vitro culture. in Genetika-Belgrade
Beograd : Društvo genetičara Srbije., 54(2), 907-919.
https://doi.org/10.2298/GENSR2202000K

Kondić-Špika A, Glogovac S, Trkulja D, Marjanović-Jeromela A, Marjanović M. Response of wheat genotypes to excess boron estimated by in vitro culture. in Genetika-Belgrade. 2022;54(2):907-919.
doi:10.2298/GENSR2202000K .

Kondić-Špika, Ankica, Glogovac, Svetlana, Trkulja, Dragana, Marjanović-Jeromela, Ana, Marjanović, Milica, "Response of wheat genotypes to excess boron estimated by in vitro culture" in Genetika-Belgrade, 54, no. 2 (2022):907-919,
https://doi.org/10.2298/GENSR2202000K . .

TY  - JOUR
AU  - Kondić-Špika, Ankica
AU  - Kobiljski, Borislav
AU  - Marjanović, Milica
AU  - Hristov, Nikola
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/874
AB  - Wheat tolerance to high boron concentrations was determined using mature embryo culture. The testing was performed on a modified MS nutrient medium to which boric acid was added in two concentrations: 15 mM and 30 mM. The control medium contained no excess boric acid. The experiment involved 14 Serbian varieties. After one month of cultivation callus fresh weight (CFW) was measured and reductions of fresh callus weight (RFCW) at boron (B) concentration of 15 mM in relation to the control were calculated. The genotypes differed significantly according to their reaction to different B concentrations. Six genotypes had RFCWs below 50.0 %, while eight genotypes had RFCWs above 50.1 %. Cultivars Nevesinjka and Pesma were considered the most tolerant genotypes, while cultivars Balada and Vila were the most sensitive. The results confirmed that differences in genotype reactions to excess B are visible at the cellular level and that they may serve as a selection criterion.
AB  - Tolerantnost pšenice prema visokim koncentracijama bora ispitana je primenom kulture zrelog embriona. Testiranje je vršeno na modifikovanoj MS hranljivoj podlozi, kojoj je borna kiselina dodata u dve koncentracije: 15 mM i 30 mM. Kontrolna podloga nije sadržala bornu kiselinu u suvišku. Eksperiment je izveden na 14 sorti pšenice proizvedenih u Srbiji. Posle mesec dana gajenja na podlogama izmerena je sveža masa kalusa (SMK) i izračunata redukcija sveže mase kalusa (RSMK) na podlozi sa 15 mM borne kiseline, u odnosu na kontrolu. Genotipovi su se značajno razlikovali u pogledu njihove reakcije na različite koncentracije bora. Šest genotipova imalo je RSMK ispod 50,0 %, dok je osam genotipova imalo RSMK iznad 50,1 %. Sorte Nevesinjka i Pesma ocenjene su kao najtolerantnije, dok su sorte Balada i Vila bile najosetljivije na prisustvo bora u podlozi. Rezultati su potvrdili da su reakcije genotipova pšenice na suvišak bora vidljive i na ćelijskom nivou, te da se ova reakcija može koristiti kao selekcioni kriterijum za procenu tolerantnosti na bor.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes
T1  - In vitro evaluacija tolerantnosti genotipova pšenice (Triticum aestivum L.) prema boru
EP  - 91
IS  - 1
SP  - 85
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_fiver_874
ER  - 

@article{
author = "Kondić-Špika, Ankica and Kobiljski, Borislav and Marjanović, Milica and Hristov, Nikola",
year = "2010",
abstract = "Wheat tolerance to high boron concentrations was determined using mature embryo culture. The testing was performed on a modified MS nutrient medium to which boric acid was added in two concentrations: 15 mM and 30 mM. The control medium contained no excess boric acid. The experiment involved 14 Serbian varieties. After one month of cultivation callus fresh weight (CFW) was measured and reductions of fresh callus weight (RFCW) at boron (B) concentration of 15 mM in relation to the control were calculated. The genotypes differed significantly according to their reaction to different B concentrations. Six genotypes had RFCWs below 50.0 %, while eight genotypes had RFCWs above 50.1 %. Cultivars Nevesinjka and Pesma were considered the most tolerant genotypes, while cultivars Balada and Vila were the most sensitive. The results confirmed that differences in genotype reactions to excess B are visible at the cellular level and that they may serve as a selection criterion., Tolerantnost pšenice prema visokim koncentracijama bora ispitana je primenom kulture zrelog embriona. Testiranje je vršeno na modifikovanoj MS hranljivoj podlozi, kojoj je borna kiselina dodata u dve koncentracije: 15 mM i 30 mM. Kontrolna podloga nije sadržala bornu kiselinu u suvišku. Eksperiment je izveden na 14 sorti pšenice proizvedenih u Srbiji. Posle mesec dana gajenja na podlogama izmerena je sveža masa kalusa (SMK) i izračunata redukcija sveže mase kalusa (RSMK) na podlozi sa 15 mM borne kiseline, u odnosu na kontrolu. Genotipovi su se značajno razlikovali u pogledu njihove reakcije na različite koncentracije bora. Šest genotipova imalo je RSMK ispod 50,0 %, dok je osam genotipova imalo RSMK iznad 50,1 %. Sorte Nevesinjka i Pesma ocenjene su kao najtolerantnije, dok su sorte Balada i Vila bile najosetljivije na prisustvo bora u podlozi. Rezultati su potvrdili da su reakcije genotipova pšenice na suvišak bora vidljive i na ćelijskom nivou, te da se ova reakcija može koristiti kao selekcioni kriterijum za procenu tolerantnosti na bor.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes, In vitro evaluacija tolerantnosti genotipova pšenice (Triticum aestivum L.) prema boru",
pages = "91-85",
number = "1",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_fiver_874"
}

Kondić-Špika, A., Kobiljski, B., Marjanović, M.,& Hristov, N.. (2010). In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 47(1), 85-91.
https://hdl.handle.net/21.15107/rcub_fiver_874

Kondić-Špika A, Kobiljski B, Marjanović M, Hristov N. In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2010;47(1):85-91.
https://hdl.handle.net/21.15107/rcub_fiver_874 .

Kondić-Špika, Ankica, Kobiljski, Borislav, Marjanović, Milica, Hristov, Nikola, "In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 47, no. 1 (2010):85-91,
https://hdl.handle.net/21.15107/rcub_fiver_874 .