TY  - JOUR
AU  - Ghimire, Krishna
AU  - Petrović, Kristina
AU  - Kontz, Brian
AU  - Bradley, Carl A.
AU  - Chilvers, Martin I.
AU  - Mueller, Daren S.
AU  - Smith, Damon L.
AU  - Wise, Kiersten A.
AU  - Mathew, Febina M.
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1915
AB  - One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P  lt  0.001) and pathogen recovery (P  lt  0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate x method interaction affected disease severity (P  lt  0.05) and pathogen recovery (P  lt  0.001). Significant differences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate x method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)
EP  - 684
IS  - 4
SP  - 677
VL  - 103
DO  - 10.1094/PDIS-06-18-1078-RE
ER  - 

@article{
author = "Ghimire, Krishna and Petrović, Kristina and Kontz, Brian and Bradley, Carl A. and Chilvers, Martin I. and Mueller, Daren S. and Smith, Damon L. and Wise, Kiersten A. and Mathew, Febina M.",
year = "2019",
abstract = "One hundred fifty-two Diaporthe isolates were recovered from symptomatic soybean (Glycine max) stems sampled from the U.S. states of Iowa, Indiana, Kentucky, Michigan, and South Dakota. Using morphology and DNA sequencing, isolates were identified as D. aspalathi (8.6%), D. caulivora (24.3%), and D. longicolla (67.1%). Aggressiveness of five isolates each of the three pathogens was studied on cultivars Hawkeye (D. caulivora and D. longicolla) and Bragg (D. aspalathi) using toothpick, stem-wound, mycelium contact, and spore injection inoculation methods in the greenhouse. For D. aspalathi, methods significantly affected disease severity (P  lt  0.001) and pathogen recovery (P  lt  0.001). The relative treatment effects (RTE) of stem-wound and toothpick methods were significantly greater than for the other methods. For D. caulivora and D. longicolla, a significant isolate x method interaction affected disease severity (P  lt  0.05) and pathogen recovery (P  lt  0.001). Significant differences in RTEs were observed among D. caulivora and D. longicolla isolates only when the stem-wound and toothpick methods were used. Our study has determined that the stem-wound and toothpick methods are reliable to evaluate the three pathogens; however, the significant isolate x method interactions for D. caulivora and D. longicolla indicate that multiple isolates should also be considered for future pathogenicity studies.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)",
pages = "684-677",
number = "4",
volume = "103",
doi = "10.1094/PDIS-06-18-1078-RE"
}

Ghimire, K., Petrović, K., Kontz, B., Bradley, C. A., Chilvers, M. I., Mueller, D. S., Smith, D. L., Wise, K. A.,& Mathew, F. M.. (2019). Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max). in Plant Disease
Amer Phytopathological Soc, St Paul., 103(4), 677-684.
https://doi.org/10.1094/PDIS-06-18-1078-RE

Ghimire K, Petrović K, Kontz B, Bradley CA, Chilvers MI, Mueller DS, Smith DL, Wise KA, Mathew FM. Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max). in Plant Disease. 2019;103(4):677-684.
doi:10.1094/PDIS-06-18-1078-RE .

Ghimire, Krishna, Petrović, Kristina, Kontz, Brian, Bradley, Carl A., Chilvers, Martin I., Mueller, Daren S., Smith, Damon L., Wise, Kiersten A., Mathew, Febina M., "Inoculation Method Impacts Symptom Development Associated with Diaporthe aspalathi, D. caulivora, and D. longicolla on Soybean (Glycine max)" in Plant Disease, 103, no. 4 (2019):677-684,
https://doi.org/10.1094/PDIS-06-18-1078-RE . .

TY  - CONF
AU  - Petrović, Kristina
AU  - Ghimire, Krishna
AU  - Okello, Paul N.
AU  - Bradley, Carl A.
AU  - Ćeran, Marina
AU  - Chilvers, Martin I.
AU  - Đorđević, Vuk
AU  - Đukić, Vojin
AU  - Miladinović, Jegor
AU  - Miladinov, Zlatica
AU  - Mueller, Daren S.
AU  - Smith, Damon L.
AU  - Balešević-Tubić, Svetlana
AU  - Vidić, Miloš
AU  - Wise, Kiersten A.
AU  - Mathew, Febina M.
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1775
PB  - Amer Phytopathological Soc, St Paul
C3  - Phytopathology
T1  - Aggressiveness evaluation of Diaporthe species causing soybean stem canker in the United States
EP  - 29
IS  - 10
SP  - 29
VL  - 108
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1775
ER  - 

@conference{
author = "Petrović, Kristina and Ghimire, Krishna and Okello, Paul N. and Bradley, Carl A. and Ćeran, Marina and Chilvers, Martin I. and Đorđević, Vuk and Đukić, Vojin and Miladinović, Jegor and Miladinov, Zlatica and Mueller, Daren S. and Smith, Damon L. and Balešević-Tubić, Svetlana and Vidić, Miloš and Wise, Kiersten A. and Mathew, Febina M.",
year = "2018",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Phytopathology",
title = "Aggressiveness evaluation of Diaporthe species causing soybean stem canker in the United States",
pages = "29-29",
number = "10",
volume = "108",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1775"
}

Petrović, K., Ghimire, K., Okello, P. N., Bradley, C. A., Ćeran, M., Chilvers, M. I., Đorđević, V., Đukić, V., Miladinović, J., Miladinov, Z., Mueller, D. S., Smith, D. L., Balešević-Tubić, S., Vidić, M., Wise, K. A.,& Mathew, F. M.. (2018). Aggressiveness evaluation of Diaporthe species causing soybean stem canker in the United States. in Phytopathology
Amer Phytopathological Soc, St Paul., 108(10), 29-29.
https://hdl.handle.net/21.15107/rcub_fiver_1775

Petrović K, Ghimire K, Okello PN, Bradley CA, Ćeran M, Chilvers MI, Đorđević V, Đukić V, Miladinović J, Miladinov Z, Mueller DS, Smith DL, Balešević-Tubić S, Vidić M, Wise KA, Mathew FM. Aggressiveness evaluation of Diaporthe species causing soybean stem canker in the United States. in Phytopathology. 2018;108(10):29-29.
https://hdl.handle.net/21.15107/rcub_fiver_1775 .

Petrović, Kristina, Ghimire, Krishna, Okello, Paul N., Bradley, Carl A., Ćeran, Marina, Chilvers, Martin I., Đorđević, Vuk, Đukić, Vojin, Miladinović, Jegor, Miladinov, Zlatica, Mueller, Daren S., Smith, Damon L., Balešević-Tubić, Svetlana, Vidić, Miloš, Wise, Kiersten A., Mathew, Febina M., "Aggressiveness evaluation of Diaporthe species causing soybean stem canker in the United States" in Phytopathology, 108, no. 10 (2018):29-29,
https://hdl.handle.net/21.15107/rcub_fiver_1775 .