TY  - CONF
AU  - Grahovac, Nada
AU  - Kondić-Špika, Ankica
AU  - Lazić, S.
AU  - Šunjka, D.
AU  - Jakšić, Snežana
AU  - Radović, B.
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3723
AB  - Residues of active substances, which were frequently found in fruits and vegetables of market producers belonged to the dithiocarbamate group pesticide. According to their use dithiocarbamates belong to fungicides in terms dithiocarbamic acid. They are a group of compounds that exist as strong complexes with various metal ions, often in a polymeric form. This makes them difficult to analyse directly because of their limited solubility in most organic solvents. It is important to have a reliable method which enables the quick and simple detection of this group for official monitoring. This work reports a simple, rapid and sensitive method for the assessment residues dithiocarbamates in fruits. Dithiocarbamates were determined indirectly by measuring the amount of carbon disulfide (CS>) that is liberated by the chemical reaction. The fruit samples in undisturbed condition were heated with a solution of stannous (II) chloride and hydrochloric acid yielding carbon disulphide. Incurred carbon disulfide (CS) are determined "head-space" technique of gas-chromatography with mass detector. Linearity was verified by using the solutions of carbon disulphide in acetone. A linear dynamic range was obtained over a range of concentrations from 0.02 to 0.12 mg/kg for carbon disulfide with correlation coefficient r > 0.995. The accuracy of the method was acceptable since the average recoveries measured at four fortification levels were in the range of 83-103% (n = 4). The precision of the developed procedure expressed as the relative standard deviations (RSDs) were lower than 3.7% in all cases. Quantification was based on external standard calibration curves made with spiked blankmatrices.
PB  - Nacionalno drugtvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad
C3  - 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEP , Vrnjačka Banja, 21 April 2013
T1  - Validation of the method for the determination of dithiocarbamates in fruits
EP  - 292
SP  - 292
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3723
ER  - 

@conference{
author = "Grahovac, Nada and Kondić-Špika, Ankica and Lazić, S. and Šunjka, D. and Jakšić, Snežana and Radović, B.",
year = "2013",
abstract = "Residues of active substances, which were frequently found in fruits and vegetables of market producers belonged to the dithiocarbamate group pesticide. According to their use dithiocarbamates belong to fungicides in terms dithiocarbamic acid. They are a group of compounds that exist as strong complexes with various metal ions, often in a polymeric form. This makes them difficult to analyse directly because of their limited solubility in most organic solvents. It is important to have a reliable method which enables the quick and simple detection of this group for official monitoring. This work reports a simple, rapid and sensitive method for the assessment residues dithiocarbamates in fruits. Dithiocarbamates were determined indirectly by measuring the amount of carbon disulfide (CS>) that is liberated by the chemical reaction. The fruit samples in undisturbed condition were heated with a solution of stannous (II) chloride and hydrochloric acid yielding carbon disulphide. Incurred carbon disulfide (CS) are determined "head-space" technique of gas-chromatography with mass detector. Linearity was verified by using the solutions of carbon disulphide in acetone. A linear dynamic range was obtained over a range of concentrations from 0.02 to 0.12 mg/kg for carbon disulfide with correlation coefficient r > 0.995. The accuracy of the method was acceptable since the average recoveries measured at four fortification levels were in the range of 83-103% (n = 4). The precision of the developed procedure expressed as the relative standard deviations (RSDs) were lower than 3.7% in all cases. Quantification was based on external standard calibration curves made with spiked blankmatrices.",
publisher = "Nacionalno drugtvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad",
journal = "3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEP , Vrnjačka Banja, 21 April 2013",
title = "Validation of the method for the determination of dithiocarbamates in fruits",
pages = "292-292",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3723"
}

Grahovac, N., Kondić-Špika, A., Lazić, S., Šunjka, D., Jakšić, S.,& Radović, B.. (2013). Validation of the method for the determination of dithiocarbamates in fruits. in 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEP , Vrnjačka Banja, 21 April 2013
Nacionalno drugtvo za procesnu tehniku i energetiku u poljoprivredi, Novi Sad., 292-292.
https://hdl.handle.net/21.15107/rcub_fiver_3723

Grahovac N, Kondić-Špika A, Lazić S, Šunjka D, Jakšić S, Radović B. Validation of the method for the determination of dithiocarbamates in fruits. in 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEP , Vrnjačka Banja, 21 April 2013. 2013;:292-292.
https://hdl.handle.net/21.15107/rcub_fiver_3723 .

Grahovac, Nada, Kondić-Špika, Ankica, Lazić, S., Šunjka, D., Jakšić, Snežana, Radović, B., "Validation of the method for the determination of dithiocarbamates in fruits" in 3rd International Conference Sustainable postharvest and Food Technolologies - INOPTEP 2013 and XXV National Conference Processing and Energy in Agriculture PTEP , Vrnjačka Banja, 21 April 2013 (2013):292-292,
https://hdl.handle.net/21.15107/rcub_fiver_3723 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Sekulić, Petar
AU  - Lazić, Sanja
AU  - Jakšić, Snežana
AU  - Šunjka, Dragana B.
AU  - Radović, B.
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1175
AB  - The use of pesticides in agriculture has brought many obvious benefits, but their inappropriate use can result in unacceptably high levels of these compounds in cereals. Pesticide residues can be found even when they are applied in accordance with good agricultural practices. A solid phase extraction (SPE) method has been developed for determination of methidathion in barley malt samples. The method involves a rapid extraction procedure with acetonitrile using salting out step. Non-polar coextractives are removed by passing a portion of the acetonitrile extract through an octadecyl (C18) solid phase extraction clean-up cartridge. An aliquot is taken and concentrated for second extract clean-up, which is done with a carbon cartridge coupled to an aminopropyl cartridge using acetonitrile: toluene 3:1 (v/v) as the elution solvent. Analysis is performed by gas chromatography, employing mass selective detection in the selected ion monitoring mode. The method rendered recoveries ranging between 91 and 96%, the associated relative standard deviations ranging between 1 and 3%. Limit of detection methidathion were less than or equal to 0.002 mg/kg. The applicability of the proposed method to detect and quantify pesticide residues has been demonstrated by the analysis of 20 genuine samples. The methidathion content in barley malt samples is not regulated by Serbian legislation (Official Gazette of RS, 28/11) and relevant EU regulation (Council Directive 90/642/EEC, 1990). The methidathion concentration found in barley malt samples were bellow the MAC value permitted by EU regulations and in accordance with the currently valid Serbian regulations for barley.
PB  - University of Novi Sad, Faculty of Technology
C3  - CEFood 2012 - Proceedings of 6th Central European Congress on Food
T1  - Determination of methidathion in barley malt by a solid phase extraction method
EP  - 581
SP  - 576
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1175
ER  - 

@conference{
author = "Grahovac, Nada and Sekulić, Petar and Lazić, Sanja and Jakšić, Snežana and Šunjka, Dragana B. and Radović, B.",
year = "2012",
abstract = "The use of pesticides in agriculture has brought many obvious benefits, but their inappropriate use can result in unacceptably high levels of these compounds in cereals. Pesticide residues can be found even when they are applied in accordance with good agricultural practices. A solid phase extraction (SPE) method has been developed for determination of methidathion in barley malt samples. The method involves a rapid extraction procedure with acetonitrile using salting out step. Non-polar coextractives are removed by passing a portion of the acetonitrile extract through an octadecyl (C18) solid phase extraction clean-up cartridge. An aliquot is taken and concentrated for second extract clean-up, which is done with a carbon cartridge coupled to an aminopropyl cartridge using acetonitrile: toluene 3:1 (v/v) as the elution solvent. Analysis is performed by gas chromatography, employing mass selective detection in the selected ion monitoring mode. The method rendered recoveries ranging between 91 and 96%, the associated relative standard deviations ranging between 1 and 3%. Limit of detection methidathion were less than or equal to 0.002 mg/kg. The applicability of the proposed method to detect and quantify pesticide residues has been demonstrated by the analysis of 20 genuine samples. The methidathion content in barley malt samples is not regulated by Serbian legislation (Official Gazette of RS, 28/11) and relevant EU regulation (Council Directive 90/642/EEC, 1990). The methidathion concentration found in barley malt samples were bellow the MAC value permitted by EU regulations and in accordance with the currently valid Serbian regulations for barley.",
publisher = "University of Novi Sad, Faculty of Technology",
journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food",
title = "Determination of methidathion in barley malt by a solid phase extraction method",
pages = "581-576",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1175"
}

Grahovac, N., Sekulić, P., Lazić, S., Jakšić, S., Šunjka, D. B.,& Radović, B.. (2012). Determination of methidathion in barley malt by a solid phase extraction method. in CEFood 2012 - Proceedings of 6th Central European Congress on Food
University of Novi Sad, Faculty of Technology., 576-581.
https://hdl.handle.net/21.15107/rcub_fiver_1175

Grahovac N, Sekulić P, Lazić S, Jakšić S, Šunjka DB, Radović B. Determination of methidathion in barley malt by a solid phase extraction method. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:576-581.
https://hdl.handle.net/21.15107/rcub_fiver_1175 .

Grahovac, Nada, Sekulić, Petar, Lazić, Sanja, Jakšić, Snežana, Šunjka, Dragana B., Radović, B., "Determination of methidathion in barley malt by a solid phase extraction method" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):576-581,
https://hdl.handle.net/21.15107/rcub_fiver_1175 .

TY  - CONF
AU  - Grahovac, Nada
AU  - Zeremski-Škorić, Tijana
AU  - Sekulić, Petar
AU  - Jakšić, Snežana
AU  - Radović, B.
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/3298
AB  - Applicability of solid phase extraction (SPE) in determination of pesticide residues in cereals
was confirmed by measuring bromophos contents in barley malt. The method entails simple extraction of pesticide
residues from sample with acetonitrile followed by solid phase extraction (SPE) clean-up step prior to the final
determination by gas chromatography with mass spectrometry . It is evaluated that performance of the method,
recoveries bromophos ranged between 80.1 and 129% with associated relative standard deviations between 2 and
13%. Limits of detection of bromophos were less or equal to 0.0013 mg/kg. The applicability of the proposed
method to detect and quantify pesticide residues has been demonstrated in the analysis of 13 real samples. The
findings for bromophos in barley malt samples were bellow the EU regulation MRL value for barley as well as in
conformance with the currently valid regulation of Serbia,
PB  - Novi Sad : University of Novi Sad, Faculty of Agriculture
C3  - Proceedings, 22nd International Symposium “Food safety production”, 19-25 June 2011, Trebinje, Bosnia and Herzegovina
T1  - Solid phase extraction in the analysis of bromophos residues in barley malt
EP  - 379
SP  - 377
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3298
ER  - 

@conference{
author = "Grahovac, Nada and Zeremski-Škorić, Tijana and Sekulić, Petar and Jakšić, Snežana and Radović, B.",
year = "2011",
abstract = "Applicability of solid phase extraction (SPE) in determination of pesticide residues in cereals
was confirmed by measuring bromophos contents in barley malt. The method entails simple extraction of pesticide
residues from sample with acetonitrile followed by solid phase extraction (SPE) clean-up step prior to the final
determination by gas chromatography with mass spectrometry . It is evaluated that performance of the method,
recoveries bromophos ranged between 80.1 and 129% with associated relative standard deviations between 2 and
13%. Limits of detection of bromophos were less or equal to 0.0013 mg/kg. The applicability of the proposed
method to detect and quantify pesticide residues has been demonstrated in the analysis of 13 real samples. The
findings for bromophos in barley malt samples were bellow the EU regulation MRL value for barley as well as in
conformance with the currently valid regulation of Serbia,",
publisher = "Novi Sad : University of Novi Sad, Faculty of Agriculture",
journal = "Proceedings, 22nd International Symposium “Food safety production”, 19-25 June 2011, Trebinje, Bosnia and Herzegovina",
title = "Solid phase extraction in the analysis of bromophos residues in barley malt",
pages = "379-377",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3298"
}

Grahovac, N., Zeremski-Škorić, T., Sekulić, P., Jakšić, S.,& Radović, B.. (2011). Solid phase extraction in the analysis of bromophos residues in barley malt. in Proceedings, 22nd International Symposium “Food safety production”, 19-25 June 2011, Trebinje, Bosnia and Herzegovina
Novi Sad : University of Novi Sad, Faculty of Agriculture., 377-379.
https://hdl.handle.net/21.15107/rcub_fiver_3298

Grahovac N, Zeremski-Škorić T, Sekulić P, Jakšić S, Radović B. Solid phase extraction in the analysis of bromophos residues in barley malt. in Proceedings, 22nd International Symposium “Food safety production”, 19-25 June 2011, Trebinje, Bosnia and Herzegovina. 2011;:377-379.
https://hdl.handle.net/21.15107/rcub_fiver_3298 .

Grahovac, Nada, Zeremski-Škorić, Tijana, Sekulić, Petar, Jakšić, Snežana, Radović, B., "Solid phase extraction in the analysis of bromophos residues in barley malt" in Proceedings, 22nd International Symposium “Food safety production”, 19-25 June 2011, Trebinje, Bosnia and Herzegovina (2011):377-379,
https://hdl.handle.net/21.15107/rcub_fiver_3298 .