TY  - JOUR
AU  - Trkulja, Dragana
AU  - Kondić-Špika, Ankica
AU  - Brbaklić, Ljiljana
AU  - Kobiljski, Borislav
AU  - Mikić, Sanja
AU  - Mirosavljević, Milan
AU  - Glogovac, Svetlana
AU  - Šurlan-Momirović, Gordana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1849
AB  - Knowledge about genetic structure and allelic diversity of bread wheat (Triticum aestivum L.) collections is a key to developing modem wheat cultivars, able to cope with various abiotic and biotic stress factors, through purposeful selection of parents. Further, information about population structure of a material is the first prerequisite of association mapping, which prevents declaring of spurious marker-trait associations. The aim of this study was to assess genetic diversity and presence of population structure within the panel of wheat accessions chosen for association mapping of yield and yield related traits in wheat. The material consisting of 282 wheat accessions, highly variable for 10 agronomically important traits, was evaluated using microsatellite markers that were distributed all over three wheat genomes. The 397 alleles were detected at 31 SSR markers, with an average number of 12.4. The highest diversity was detected at microsatellite loci from B genome, while the lowest number of alleles was observed for D genome. Structure analysis indicated the existence of three subpopulations, where genotypes were grouped according to their origin as well as pedigree data. In each subpopulation, private alleles were detected, indicating informativeness of analysed microsatellite loci for the elucidation of population structure. Statistically significant differences among groups were observed for 8 out of 10 wheat agronomically important traits, while PCA did not show a clear separation of genotypes. The analysed wheat accessions demonstrated a sufficiently high level of genetic diversity, considering both molecular and phenotypic data, which makes them suitable for the association study of breeding traits in wheat.
PB  - Lithuanian Research Centre Agriculture & Forestry, Kedainiu R
T2  - Zemdirbyste-Agriculture
T1  - Genetic structure and allelic richness of the wheat core collection for association mapping of yield
EP  - 264
IS  - 3
SP  - 257
VL  - 106
DO  - 10.13080/z-a.2019.106.033
ER  - 

@article{
author = "Trkulja, Dragana and Kondić-Špika, Ankica and Brbaklić, Ljiljana and Kobiljski, Borislav and Mikić, Sanja and Mirosavljević, Milan and Glogovac, Svetlana and Šurlan-Momirović, Gordana",
year = "2019",
abstract = "Knowledge about genetic structure and allelic diversity of bread wheat (Triticum aestivum L.) collections is a key to developing modem wheat cultivars, able to cope with various abiotic and biotic stress factors, through purposeful selection of parents. Further, information about population structure of a material is the first prerequisite of association mapping, which prevents declaring of spurious marker-trait associations. The aim of this study was to assess genetic diversity and presence of population structure within the panel of wheat accessions chosen for association mapping of yield and yield related traits in wheat. The material consisting of 282 wheat accessions, highly variable for 10 agronomically important traits, was evaluated using microsatellite markers that were distributed all over three wheat genomes. The 397 alleles were detected at 31 SSR markers, with an average number of 12.4. The highest diversity was detected at microsatellite loci from B genome, while the lowest number of alleles was observed for D genome. Structure analysis indicated the existence of three subpopulations, where genotypes were grouped according to their origin as well as pedigree data. In each subpopulation, private alleles were detected, indicating informativeness of analysed microsatellite loci for the elucidation of population structure. Statistically significant differences among groups were observed for 8 out of 10 wheat agronomically important traits, while PCA did not show a clear separation of genotypes. The analysed wheat accessions demonstrated a sufficiently high level of genetic diversity, considering both molecular and phenotypic data, which makes them suitable for the association study of breeding traits in wheat.",
publisher = "Lithuanian Research Centre Agriculture & Forestry, Kedainiu R",
journal = "Zemdirbyste-Agriculture",
title = "Genetic structure and allelic richness of the wheat core collection for association mapping of yield",
pages = "264-257",
number = "3",
volume = "106",
doi = "10.13080/z-a.2019.106.033"
}

Trkulja, D., Kondić-Špika, A., Brbaklić, L., Kobiljski, B., Mikić, S., Mirosavljević, M., Glogovac, S.,& Šurlan-Momirović, G.. (2019). Genetic structure and allelic richness of the wheat core collection for association mapping of yield. in Zemdirbyste-Agriculture
Lithuanian Research Centre Agriculture & Forestry, Kedainiu R., 106(3), 257-264.
https://doi.org/10.13080/z-a.2019.106.033

Trkulja D, Kondić-Špika A, Brbaklić L, Kobiljski B, Mikić S, Mirosavljević M, Glogovac S, Šurlan-Momirović G. Genetic structure and allelic richness of the wheat core collection for association mapping of yield. in Zemdirbyste-Agriculture. 2019;106(3):257-264.
doi:10.13080/z-a.2019.106.033 .

Trkulja, Dragana, Kondić-Špika, Ankica, Brbaklić, Ljiljana, Kobiljski, Borislav, Mikić, Sanja, Mirosavljević, Milan, Glogovac, Svetlana, Šurlan-Momirović, Gordana, "Genetic structure and allelic richness of the wheat core collection for association mapping of yield" in Zemdirbyste-Agriculture, 106, no. 3 (2019):257-264,
https://doi.org/10.13080/z-a.2019.106.033 . .

TY  - CONF
AU  - Kondić-Špika, Ankica
AU  - Mikić, Sanja
AU  - Trkulja, Dragana
AU  - Marjanović-Jeromela, Ana
AU  - Denčić, Srbislav
AU  - Kobiljski, Borislav
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/2247
AB  - Phenotypic, biochemical and molecular evaluations of wheat collections are of a great importance to increase the knowledge of genetic diversity as the basic prerequisite for crop improvement in diff erent breeding programs. Large scale genotyping by molecular markers as well as phenotyping of agronomical important traits generated a lot of valuable information for wheat researchers during the last few decades. However, some morphological traits are almost forgotten and very rarely used in evaluating diversity of wheat germplasm. The aim of this study was to analyse the morphological diversity in a collection of 450 wheat accessions originating from all over the world. The genotypes were chosen from the wheat genetic collection of the Small Grains Department and sown at the experimental field of the Institute of Field and Vegetable Crops, location of Rimski Šančevi (45°20`N, 19°51`E). Five morphological traits were analysed and used as markers for distinctness of wheat genotypes: auricle colour (AC), coleoptile colour (CC), leaf colour (LC), colour at tillering time (CTT) and grain colour (GC). The Shannon diversity index (H) was estimated as a measure of morphological diversity. The results have shown that the most of the genotypes had white auricle colour (87%), white coleoptile colour (81%), dark green colour at tillering time (96%), green leaf colour (61%) and light red grain colour (43%). The average value for the Shannon diversity index was 0,77 indicating high level of morphological diversity in the collection. The lowest diversity (0,07) was found for CCT and the highest individual descriptor diversity was found for GC (0,68). The results indicated that morphological characterization with higer number of analysed traits can be used to improve description of wheat germplasm in addition to molecular and agronomical evaluations.
PB  - Belgrade : University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden Jevremovac
C3  - Book of Abstracts, 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia, Botanica Serbica 42 (supplement 1)
T1  - Diversity of wheat genotypes based on morphological markers
EP  - 166
SP  - 165
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2247
ER  - 

@conference{
author = "Kondić-Špika, Ankica and Mikić, Sanja and Trkulja, Dragana and Marjanović-Jeromela, Ana and Denčić, Srbislav and Kobiljski, Borislav",
year = "2018",
abstract = "Phenotypic, biochemical and molecular evaluations of wheat collections are of a great importance to increase the knowledge of genetic diversity as the basic prerequisite for crop improvement in diff erent breeding programs. Large scale genotyping by molecular markers as well as phenotyping of agronomical important traits generated a lot of valuable information for wheat researchers during the last few decades. However, some morphological traits are almost forgotten and very rarely used in evaluating diversity of wheat germplasm. The aim of this study was to analyse the morphological diversity in a collection of 450 wheat accessions originating from all over the world. The genotypes were chosen from the wheat genetic collection of the Small Grains Department and sown at the experimental field of the Institute of Field and Vegetable Crops, location of Rimski Šančevi (45°20`N, 19°51`E). Five morphological traits were analysed and used as markers for distinctness of wheat genotypes: auricle colour (AC), coleoptile colour (CC), leaf colour (LC), colour at tillering time (CTT) and grain colour (GC). The Shannon diversity index (H) was estimated as a measure of morphological diversity. The results have shown that the most of the genotypes had white auricle colour (87%), white coleoptile colour (81%), dark green colour at tillering time (96%), green leaf colour (61%) and light red grain colour (43%). The average value for the Shannon diversity index was 0,77 indicating high level of morphological diversity in the collection. The lowest diversity (0,07) was found for CCT and the highest individual descriptor diversity was found for GC (0,68). The results indicated that morphological characterization with higer number of analysed traits can be used to improve description of wheat germplasm in addition to molecular and agronomical evaluations.",
publisher = "Belgrade : University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden Jevremovac",
journal = "Book of Abstracts, 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia, Botanica Serbica 42 (supplement 1)",
title = "Diversity of wheat genotypes based on morphological markers",
pages = "166-165",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2247"
}

Kondić-Špika, A., Mikić, S., Trkulja, D., Marjanović-Jeromela, A., Denčić, S.,& Kobiljski, B.. (2018). Diversity of wheat genotypes based on morphological markers. in Book of Abstracts, 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia, Botanica Serbica 42 (supplement 1)
Belgrade : University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden Jevremovac., 165-166.
https://hdl.handle.net/21.15107/rcub_fiver_2247

Kondić-Špika A, Mikić S, Trkulja D, Marjanović-Jeromela A, Denčić S, Kobiljski B. Diversity of wheat genotypes based on morphological markers. in Book of Abstracts, 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia, Botanica Serbica 42 (supplement 1). 2018;:165-166.
https://hdl.handle.net/21.15107/rcub_fiver_2247 .

Kondić-Špika, Ankica, Mikić, Sanja, Trkulja, Dragana, Marjanović-Jeromela, Ana, Denčić, Srbislav, Kobiljski, Borislav, "Diversity of wheat genotypes based on morphological markers" in Book of Abstracts, 7th Balkan Botanical Congress, 10-14th September 2018, Novi Sad, Serbia, Botanica Serbica 42 (supplement 1) (2018):165-166,
https://hdl.handle.net/21.15107/rcub_fiver_2247 .

TY  - JOUR
AU  - Brdar-Jokanović, Milka
AU  - Zorić, Miroslav
AU  - Kondić-Špika, Ankica
AU  - Maksimović, Ivana
AU  - Kobiljski, Borislav
AU  - Kraljević-Balalić, Marija
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1667
AB  - This study was aimed to assess the effects of excess boron on 59 genetically divergent wheat accessions and to identify those with high and stable yields under a range of soil boron concentrations. The second aim was to test the applicability of a laboratory technique performed at juvenile stages of development in estimating field boron tolerance. The study comprised a control and three boron treatments, applied as 50, 100 and 150 mg boric acid L-1 in laboratory, and 33.0, 67.0 and 133.0 kg boric acid ha(-1) in field trial. Yield performance and stability were evaluated using biplots from sites regression model, while interrelationships among analyzed parameters were assessed using path coefficient analysis. Parameters were mostly decreased by excess boron when compared to the control (seedling root length, seedling dry weight, grain number per spike, grain yield, flag leaf area, leaf area duration and grain weight). Significant increase was noted for seedling boron concentration and content, percentage of sterile spikelets per spike and number of spikes per m(2). Spike length, number of spikelets per spike, and anthesis date remained unaffected. The majority of accessions with high and stable yields were of local origin, so, we conclude that adaptation to environmental factors other than elevated soil boron plays an important role in overall field boron tolerance. The effects of excessive external boron on boron accumulation noted at the seedling stage in laboratory studies corresponded to its effects on yield in field.
PB  - Tarbiat Modares Univ, Tehran
T2  - Journal of Agricultural Science & Technology
T1  - Boron Tolerance in Wheat Accessions of Different Origin Estimated in Controlled and Field Conditions
EP  - 356
IS  - 2
SP  - 345
VL  - 19
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1667
ER  - 

@article{
author = "Brdar-Jokanović, Milka and Zorić, Miroslav and Kondić-Špika, Ankica and Maksimović, Ivana and Kobiljski, Borislav and Kraljević-Balalić, Marija",
year = "2017",
abstract = "This study was aimed to assess the effects of excess boron on 59 genetically divergent wheat accessions and to identify those with high and stable yields under a range of soil boron concentrations. The second aim was to test the applicability of a laboratory technique performed at juvenile stages of development in estimating field boron tolerance. The study comprised a control and three boron treatments, applied as 50, 100 and 150 mg boric acid L-1 in laboratory, and 33.0, 67.0 and 133.0 kg boric acid ha(-1) in field trial. Yield performance and stability were evaluated using biplots from sites regression model, while interrelationships among analyzed parameters were assessed using path coefficient analysis. Parameters were mostly decreased by excess boron when compared to the control (seedling root length, seedling dry weight, grain number per spike, grain yield, flag leaf area, leaf area duration and grain weight). Significant increase was noted for seedling boron concentration and content, percentage of sterile spikelets per spike and number of spikes per m(2). Spike length, number of spikelets per spike, and anthesis date remained unaffected. The majority of accessions with high and stable yields were of local origin, so, we conclude that adaptation to environmental factors other than elevated soil boron plays an important role in overall field boron tolerance. The effects of excessive external boron on boron accumulation noted at the seedling stage in laboratory studies corresponded to its effects on yield in field.",
publisher = "Tarbiat Modares Univ, Tehran",
journal = "Journal of Agricultural Science & Technology",
title = "Boron Tolerance in Wheat Accessions of Different Origin Estimated in Controlled and Field Conditions",
pages = "356-345",
number = "2",
volume = "19",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1667"
}

Brdar-Jokanović, M., Zorić, M., Kondić-Špika, A., Maksimović, I., Kobiljski, B.,& Kraljević-Balalić, M.. (2017). Boron Tolerance in Wheat Accessions of Different Origin Estimated in Controlled and Field Conditions. in Journal of Agricultural Science & Technology
Tarbiat Modares Univ, Tehran., 19(2), 345-356.
https://hdl.handle.net/21.15107/rcub_fiver_1667

Brdar-Jokanović M, Zorić M, Kondić-Špika A, Maksimović I, Kobiljski B, Kraljević-Balalić M. Boron Tolerance in Wheat Accessions of Different Origin Estimated in Controlled and Field Conditions. in Journal of Agricultural Science & Technology. 2017;19(2):345-356.
https://hdl.handle.net/21.15107/rcub_fiver_1667 .

Brdar-Jokanović, Milka, Zorić, Miroslav, Kondić-Špika, Ankica, Maksimović, Ivana, Kobiljski, Borislav, Kraljević-Balalić, Marija, "Boron Tolerance in Wheat Accessions of Different Origin Estimated in Controlled and Field Conditions" in Journal of Agricultural Science & Technology, 19, no. 2 (2017):345-356,
https://hdl.handle.net/21.15107/rcub_fiver_1667 .

TY  - JOUR
AU  - Mikić, Sanja
AU  - Zorić, Miroslav
AU  - Stanisavljević, Dušan
AU  - Kondić-Špika, Ankica
AU  - Brbaklić, Ljiljana
AU  - Kobiljski, Borislav
AU  - Nastasić, Aleksandra
AU  - Mitrović, Bojan
AU  - Šurlan-Momirović, Gordana
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1584
AB  - Drought is a severe threat to maize yield stability in Serbia and other temperate Southeast European countries occurring occasionally but with significant yield losses. The development of resilient genotypes that perform well under drought is one of the main focuses of maize breeding programmes. To test the tolerance of newly developed elite maize inbred lines to drought stress, field trials for grain yield performance and anthesis silk interval (ASI) were set in drought stressed environments in 2011 and 2012. Inbred lines performing well under drought, clustered into a group with short ASI and a smaller group with long ASI, were considered as a potential source for tolerance. The former contained inbreds from different heterotic groups and with a proportion of local germplasm. The latter consisted of genotypes with mixed exotic and Lancaster germplasm, which performed better in more drought-affected environments. Three inbreds were selected for their potential drought tolerance, showing an above-average yield and small ASI in all environments. Association analysis indicated significant correlations between ASI and grain yield and three microsatellites (bnlg1525, bnlg238 and umc1025). Eight alleles were selected for their favourable concurrent effect on yield increase and ASI decrease. The proportion of phenotypic variation explained by the markers varied across environments from 5.7% to 22.4% and from 4.6% to 8.1% for ASI and yield, respectively. The alleles with strongest effect on performance of particular genotypes and their interactions in specific environments were identified by the mean of partial least square interactions analysis indicating potential suitability of the makers for tolerant genotype selection.
PB  - Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid
T2  - Spanish Journal of Agricultural Research
T1  - Agronomic and molecular evaluation of maize inbred lines for drought tolerance
IS  - 4
VL  - 14
DO  - 10.5424/sjar/2016144-9116
ER  - 

@article{
author = "Mikić, Sanja and Zorić, Miroslav and Stanisavljević, Dušan and Kondić-Špika, Ankica and Brbaklić, Ljiljana and Kobiljski, Borislav and Nastasić, Aleksandra and Mitrović, Bojan and Šurlan-Momirović, Gordana",
year = "2016",
abstract = "Drought is a severe threat to maize yield stability in Serbia and other temperate Southeast European countries occurring occasionally but with significant yield losses. The development of resilient genotypes that perform well under drought is one of the main focuses of maize breeding programmes. To test the tolerance of newly developed elite maize inbred lines to drought stress, field trials for grain yield performance and anthesis silk interval (ASI) were set in drought stressed environments in 2011 and 2012. Inbred lines performing well under drought, clustered into a group with short ASI and a smaller group with long ASI, were considered as a potential source for tolerance. The former contained inbreds from different heterotic groups and with a proportion of local germplasm. The latter consisted of genotypes with mixed exotic and Lancaster germplasm, which performed better in more drought-affected environments. Three inbreds were selected for their potential drought tolerance, showing an above-average yield and small ASI in all environments. Association analysis indicated significant correlations between ASI and grain yield and three microsatellites (bnlg1525, bnlg238 and umc1025). Eight alleles were selected for their favourable concurrent effect on yield increase and ASI decrease. The proportion of phenotypic variation explained by the markers varied across environments from 5.7% to 22.4% and from 4.6% to 8.1% for ASI and yield, respectively. The alleles with strongest effect on performance of particular genotypes and their interactions in specific environments were identified by the mean of partial least square interactions analysis indicating potential suitability of the makers for tolerant genotype selection.",
publisher = "Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid",
journal = "Spanish Journal of Agricultural Research",
title = "Agronomic and molecular evaluation of maize inbred lines for drought tolerance",
number = "4",
volume = "14",
doi = "10.5424/sjar/2016144-9116"
}

Mikić, S., Zorić, M., Stanisavljević, D., Kondić-Špika, A., Brbaklić, L., Kobiljski, B., Nastasić, A., Mitrović, B.,& Šurlan-Momirović, G.. (2016). Agronomic and molecular evaluation of maize inbred lines for drought tolerance. in Spanish Journal of Agricultural Research
Spanish Natl Inst Agricultural & Food Research & Technolo, Madrid., 14(4).
https://doi.org/10.5424/sjar/2016144-9116

Mikić S, Zorić M, Stanisavljević D, Kondić-Špika A, Brbaklić L, Kobiljski B, Nastasić A, Mitrović B, Šurlan-Momirović G. Agronomic and molecular evaluation of maize inbred lines for drought tolerance. in Spanish Journal of Agricultural Research. 2016;14(4).
doi:10.5424/sjar/2016144-9116 .

Mikić, Sanja, Zorić, Miroslav, Stanisavljević, Dušan, Kondić-Špika, Ankica, Brbaklić, Ljiljana, Kobiljski, Borislav, Nastasić, Aleksandra, Mitrović, Bojan, Šurlan-Momirović, Gordana, "Agronomic and molecular evaluation of maize inbred lines for drought tolerance" in Spanish Journal of Agricultural Research, 14, no. 4 (2016),
https://doi.org/10.5424/sjar/2016144-9116 . .

TY  - JOUR
AU  - Mikić, Sanja
AU  - Kondić-Špika, Ankica
AU  - Brbaklić, Ljiljana
AU  - Stanisavljević, Dušan
AU  - Trkulja, Dragana
AU  - Tomičić, Marina
AU  - Nastasić, Aleksandra
AU  - Kobiljski, Borislav
AU  - Prodanović, Slaven
AU  - Šurlan-Momirović, Gordana
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1587
AB  - Association analysis is a relatively novel approach in quantitative traits studies that allows high resolution mapping and time efficient and direct application on breeding material. Since the markers, which are close to the quantitative trait loci stable across environments and genetic backgrounds, may be valuable for marker assisted selection, we chose microsatellite markers previously linked to traits of interest in various mapping studies. A set of 36 microsatellite markers positioned near important maize (Zea mays L.) agronomic loci was used to evaluate genetic diversity and determine population structure. To verify the associations between the markers and traits, a panel of diverse maize inbred lines was genotyped with microsatellites and phenotyped for flowering time, yield and yield components. A relatively high level of polymorphism detected in number of alleles per locus (8.2), average polymorphic information content value (0.64), and average gene diversity (0.684) lines showed the analyzed panel of maize inbred contained significant genetic diversity and was suitable for association mapping. The population structure estimated by model-based clustering method grouped maize inbred lines into three clusters. The association analysis using the general linear and mixed linear models determined significant correlations between several agronomic traits and three microsatellites on chromosomes 3, 5, and 8, namely umc1025, bnlg1237, and bnlg162 consistent across the environments, explaining from 4.7% to 18.2% of total phenotypic variations. The results suggest that the chromosome regions containing quantitative trait loci (QTLs) associated with multiple yield-related traits consistently across environments are potentially important targets for selection.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Multiple marker-traits associations for maize agronomic traits
EP  - 306
IS  - 3
SP  - 300
VL  - 76
DO  - 10.4067/S0718-58392016000300006
ER  - 

@article{
author = "Mikić, Sanja and Kondić-Špika, Ankica and Brbaklić, Ljiljana and Stanisavljević, Dušan and Trkulja, Dragana and Tomičić, Marina and Nastasić, Aleksandra and Kobiljski, Borislav and Prodanović, Slaven and Šurlan-Momirović, Gordana",
year = "2016",
abstract = "Association analysis is a relatively novel approach in quantitative traits studies that allows high resolution mapping and time efficient and direct application on breeding material. Since the markers, which are close to the quantitative trait loci stable across environments and genetic backgrounds, may be valuable for marker assisted selection, we chose microsatellite markers previously linked to traits of interest in various mapping studies. A set of 36 microsatellite markers positioned near important maize (Zea mays L.) agronomic loci was used to evaluate genetic diversity and determine population structure. To verify the associations between the markers and traits, a panel of diverse maize inbred lines was genotyped with microsatellites and phenotyped for flowering time, yield and yield components. A relatively high level of polymorphism detected in number of alleles per locus (8.2), average polymorphic information content value (0.64), and average gene diversity (0.684) lines showed the analyzed panel of maize inbred contained significant genetic diversity and was suitable for association mapping. The population structure estimated by model-based clustering method grouped maize inbred lines into three clusters. The association analysis using the general linear and mixed linear models determined significant correlations between several agronomic traits and three microsatellites on chromosomes 3, 5, and 8, namely umc1025, bnlg1237, and bnlg162 consistent across the environments, explaining from 4.7% to 18.2% of total phenotypic variations. The results suggest that the chromosome regions containing quantitative trait loci (QTLs) associated with multiple yield-related traits consistently across environments are potentially important targets for selection.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Multiple marker-traits associations for maize agronomic traits",
pages = "306-300",
number = "3",
volume = "76",
doi = "10.4067/S0718-58392016000300006"
}

Mikić, S., Kondić-Špika, A., Brbaklić, L., Stanisavljević, D., Trkulja, D., Tomičić, M., Nastasić, A., Kobiljski, B., Prodanović, S.,& Šurlan-Momirović, G.. (2016). Multiple marker-traits associations for maize agronomic traits. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 76(3), 300-306.
https://doi.org/10.4067/S0718-58392016000300006

Mikić S, Kondić-Špika A, Brbaklić L, Stanisavljević D, Trkulja D, Tomičić M, Nastasić A, Kobiljski B, Prodanović S, Šurlan-Momirović G. Multiple marker-traits associations for maize agronomic traits. in Chilean Journal of Agricultural Research. 2016;76(3):300-306.
doi:10.4067/S0718-58392016000300006 .

Mikić, Sanja, Kondić-Špika, Ankica, Brbaklić, Ljiljana, Stanisavljević, Dušan, Trkulja, Dragana, Tomičić, Marina, Nastasić, Aleksandra, Kobiljski, Borislav, Prodanović, Slaven, Šurlan-Momirović, Gordana, "Multiple marker-traits associations for maize agronomic traits" in Chilean Journal of Agricultural Research, 76, no. 3 (2016):300-306,
https://doi.org/10.4067/S0718-58392016000300006 . .

TY  - CONF
AU  - Mikić, Sanja
AU  - Kondić-Špika, Ankica
AU  - Brbaklić, Ljiljana
AU  - Stanisavljević, Dušan
AU  - Trkulja, Dragana
AU  - Tomičić, Marina
AU  - Kobiljski, Borislav
AU  - Prodanović, Slaven
AU  - Šurlan-Momirović, Gordana
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/2728
AB  - Association analysis, a recent approach in studying genetic architecture of quantitative traits, provides higher resolution mapping, time efficient and direct application on breeding material in comparison to biparental QTL mapping. Assuming that markers near stable QTLs across different environments and genetic backgrounds can be useful for marker-assisted selection, we selected SSR markers previously associated with the QTLs for the traits of interest in various mapping studies. To confirm the marker trait associations and evaluate the stability of the QTLs, a set of diverse maize inbred lines developed at the Institute of Field and Vegetable Crops in Novi Sad was chosen. It was genotyped with microsatellite markers near already known QTLs and phenotyped for flowering time, yield and yield components. Association analysis indicated significant correlations between several agronomic traits and three microsatellites on chromosomes 3, 5 and 8, namely umc1025, bnlg1237 and bnlg162.
PB  - Belgrade : Serbian Plant Physiology Society
PB  - Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“
C3  - Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia
T1  - Associations between SSR markers and multiple important agronomic traits in maize
EP  - 26
SP  - 26
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2728
ER  - 

@conference{
author = "Mikić, Sanja and Kondić-Špika, Ankica and Brbaklić, Ljiljana and Stanisavljević, Dušan and Trkulja, Dragana and Tomičić, Marina and Kobiljski, Borislav and Prodanović, Slaven and Šurlan-Momirović, Gordana",
year = "2015",
abstract = "Association analysis, a recent approach in studying genetic architecture of quantitative traits, provides higher resolution mapping, time efficient and direct application on breeding material in comparison to biparental QTL mapping. Assuming that markers near stable QTLs across different environments and genetic backgrounds can be useful for marker-assisted selection, we selected SSR markers previously associated with the QTLs for the traits of interest in various mapping studies. To confirm the marker trait associations and evaluate the stability of the QTLs, a set of diverse maize inbred lines developed at the Institute of Field and Vegetable Crops in Novi Sad was chosen. It was genotyped with microsatellite markers near already known QTLs and phenotyped for flowering time, yield and yield components. Association analysis indicated significant correlations between several agronomic traits and three microsatellites on chromosomes 3, 5 and 8, namely umc1025, bnlg1237 and bnlg162.",
publisher = "Belgrade : Serbian Plant Physiology Society, Belgrade : University of Belgrade, Institute for Biological Research „Siniša Stanković“",
journal = "Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia",
title = "Associations between SSR markers and multiple important agronomic traits in maize",
pages = "26-26",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2728"
}

Mikić, S., Kondić-Špika, A., Brbaklić, L., Stanisavljević, D., Trkulja, D., Tomičić, M., Kobiljski, B., Prodanović, S.,& Šurlan-Momirović, G.. (2015). Associations between SSR markers and multiple important agronomic traits in maize. in Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia
Belgrade : Serbian Plant Physiology Society., 26-26.
https://hdl.handle.net/21.15107/rcub_fiver_2728

Mikić S, Kondić-Špika A, Brbaklić L, Stanisavljević D, Trkulja D, Tomičić M, Kobiljski B, Prodanović S, Šurlan-Momirović G. Associations between SSR markers and multiple important agronomic traits in maize. in Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia. 2015;:26-26.
https://hdl.handle.net/21.15107/rcub_fiver_2728 .

Mikić, Sanja, Kondić-Špika, Ankica, Brbaklić, Ljiljana, Stanisavljević, Dušan, Trkulja, Dragana, Tomičić, Marina, Kobiljski, Borislav, Prodanović, Slaven, Šurlan-Momirović, Gordana, "Associations between SSR markers and multiple important agronomic traits in maize" in Book of Abstracts, 2nd International Conference on Plant Biology, 21st Symposium of the Serbian Plant Physiology Society, 17-20.06.2015., Petnica Science Center, Serbia (2015):26-26,
https://hdl.handle.net/21.15107/rcub_fiver_2728 .

TY  - JOUR
AU  - Brbaklić, Ljiljana
AU  - Trkulja, Dragana
AU  - Kondić-Špika, Ankica
AU  - Mikić, Sanja
AU  - Tomičić, Marina
AU  - Kobiljski, Borislav
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1490
AB  - The microsatellites, as one of the most robust markers for identification of wheat varieties, were used for assessment of genetic diversity and population structure to promote effective use of genetic resources. In this study, the set of 284 wheat varieties were genotyped using 30 microsatellite markers. The chosen SSR markers were located among almost all linkage groups and covered all three genomes. The genotypes used originate from 24 different breeding centers worldwide and are included in an extensive core collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. The total number of detected alleles was 349 at all analyzed loci. The average number of detected allelic variant per locus was 11.5. The mean value of polymorphic information content was 0.68. According to the probability of data obtained by program Structure, the results have shown presence of 6 subpopulations within the studied set of genotypes. The population structure positively correlated to some extent with geographic origin. The available pedigree data were included for additional explanation of population structure. The results of this study should provide valuable information for future association studies using the diverse wheat breeding material.
PB  - Akademiai Kiado Zrt, Budapest
T2  - Cereal Research Communications
T1  - Determination of Population Structure of Wheat Core Collection for Association Mapping
EP  - 28
IS  - 1
SP  - 22
VL  - 43
DO  - 10.1556/CRC.2014.0027
ER  - 

@article{
author = "Brbaklić, Ljiljana and Trkulja, Dragana and Kondić-Špika, Ankica and Mikić, Sanja and Tomičić, Marina and Kobiljski, Borislav",
year = "2015",
abstract = "The microsatellites, as one of the most robust markers for identification of wheat varieties, were used for assessment of genetic diversity and population structure to promote effective use of genetic resources. In this study, the set of 284 wheat varieties were genotyped using 30 microsatellite markers. The chosen SSR markers were located among almost all linkage groups and covered all three genomes. The genotypes used originate from 24 different breeding centers worldwide and are included in an extensive core collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. The total number of detected alleles was 349 at all analyzed loci. The average number of detected allelic variant per locus was 11.5. The mean value of polymorphic information content was 0.68. According to the probability of data obtained by program Structure, the results have shown presence of 6 subpopulations within the studied set of genotypes. The population structure positively correlated to some extent with geographic origin. The available pedigree data were included for additional explanation of population structure. The results of this study should provide valuable information for future association studies using the diverse wheat breeding material.",
publisher = "Akademiai Kiado Zrt, Budapest",
journal = "Cereal Research Communications",
title = "Determination of Population Structure of Wheat Core Collection for Association Mapping",
pages = "28-22",
number = "1",
volume = "43",
doi = "10.1556/CRC.2014.0027"
}

Brbaklić, L., Trkulja, D., Kondić-Špika, A., Mikić, S., Tomičić, M.,& Kobiljski, B.. (2015). Determination of Population Structure of Wheat Core Collection for Association Mapping. in Cereal Research Communications
Akademiai Kiado Zrt, Budapest., 43(1), 22-28.
https://doi.org/10.1556/CRC.2014.0027

Brbaklić L, Trkulja D, Kondić-Špika A, Mikić S, Tomičić M, Kobiljski B. Determination of Population Structure of Wheat Core Collection for Association Mapping. in Cereal Research Communications. 2015;43(1):22-28.
doi:10.1556/CRC.2014.0027 .

Brbaklić, Ljiljana, Trkulja, Dragana, Kondić-Špika, Ankica, Mikić, Sanja, Tomičić, Marina, Kobiljski, Borislav, "Determination of Population Structure of Wheat Core Collection for Association Mapping" in Cereal Research Communications, 43, no. 1 (2015):22-28,
https://doi.org/10.1556/CRC.2014.0027 . .

TY  - JOUR
AU  - Brbaklić, Ljiljana
AU  - Trkulja, Dragana
AU  - Kondić-Špika, Ankica
AU  - Hristov, Nikola
AU  - Denčić, Srbislav
AU  - Mikić, Sanja
AU  - Tomičić, Marina
AU  - Kobiljski, Borislav
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1441
AB  - The objective of this work was to assess the genetic diversity and population structure of wheat genotypes, to detect significant and stable genetic associations, as well as to evaluate the efficiency of statistical models to identify chromosome regions responsible for the expression of spike-related traits. Eight important spike characteristics were measured during five growing seasons in Serbia. A set of 30 microsatellite markers positioned near important agronomic loci was used to evaluate genetic diversity, resulting in a total of 349 alleles. The marker-trait associations were analyzed using the general linear and mixed linear models. The results obtained for number of allelic variants per locus (11.5), average polymorphic information content value (0.68), and average gene diversity (0.722) showed that the exceptional level of polymorphism in the genotypes is the main requirement for association studies. The population structure estimated by model-based clustering distributed the genotypes into six subpopulations according to log probability of data. Significant and stable associations were detected on chromosomes 1B, 2A, 2B, 2D, and 6D, which explained from 4.7 to 40.7% of total phenotypic variations. The general linear model identified a significantly larger number of marker-trait associations (192) than the mixed linear model (76). The mixed linear model identified nine markers associated to six traits.
PB  - Empresa Brasil Pesq Agropec, Brasilia Df
T2  - Pesquisa Agropecuaria Brasileira
T1  - Genetic associations in the detection of QTLs for wheat spike-related traits
EP  - 159
IS  - 2
SP  - 149
VL  - 50
DO  - 10.1590/S0100-204X2015000200007
ER  - 

@article{
author = "Brbaklić, Ljiljana and Trkulja, Dragana and Kondić-Špika, Ankica and Hristov, Nikola and Denčić, Srbislav and Mikić, Sanja and Tomičić, Marina and Kobiljski, Borislav",
year = "2015",
abstract = "The objective of this work was to assess the genetic diversity and population structure of wheat genotypes, to detect significant and stable genetic associations, as well as to evaluate the efficiency of statistical models to identify chromosome regions responsible for the expression of spike-related traits. Eight important spike characteristics were measured during five growing seasons in Serbia. A set of 30 microsatellite markers positioned near important agronomic loci was used to evaluate genetic diversity, resulting in a total of 349 alleles. The marker-trait associations were analyzed using the general linear and mixed linear models. The results obtained for number of allelic variants per locus (11.5), average polymorphic information content value (0.68), and average gene diversity (0.722) showed that the exceptional level of polymorphism in the genotypes is the main requirement for association studies. The population structure estimated by model-based clustering distributed the genotypes into six subpopulations according to log probability of data. Significant and stable associations were detected on chromosomes 1B, 2A, 2B, 2D, and 6D, which explained from 4.7 to 40.7% of total phenotypic variations. The general linear model identified a significantly larger number of marker-trait associations (192) than the mixed linear model (76). The mixed linear model identified nine markers associated to six traits.",
publisher = "Empresa Brasil Pesq Agropec, Brasilia Df",
journal = "Pesquisa Agropecuaria Brasileira",
title = "Genetic associations in the detection of QTLs for wheat spike-related traits",
pages = "159-149",
number = "2",
volume = "50",
doi = "10.1590/S0100-204X2015000200007"
}

Brbaklić, L., Trkulja, D., Kondić-Špika, A., Hristov, N., Denčić, S., Mikić, S., Tomičić, M.,& Kobiljski, B.. (2015). Genetic associations in the detection of QTLs for wheat spike-related traits. in Pesquisa Agropecuaria Brasileira
Empresa Brasil Pesq Agropec, Brasilia Df., 50(2), 149-159.
https://doi.org/10.1590/S0100-204X2015000200007

Brbaklić L, Trkulja D, Kondić-Špika A, Hristov N, Denčić S, Mikić S, Tomičić M, Kobiljski B. Genetic associations in the detection of QTLs for wheat spike-related traits. in Pesquisa Agropecuaria Brasileira. 2015;50(2):149-159.
doi:10.1590/S0100-204X2015000200007 .

Brbaklić, Ljiljana, Trkulja, Dragana, Kondić-Špika, Ankica, Hristov, Nikola, Denčić, Srbislav, Mikić, Sanja, Tomičić, Marina, Kobiljski, Borislav, "Genetic associations in the detection of QTLs for wheat spike-related traits" in Pesquisa Agropecuaria Brasileira, 50, no. 2 (2015):149-159,
https://doi.org/10.1590/S0100-204X2015000200007 . .

TY  - CONF
AU  - Mihailović, Vojislav
AU  - Mikić, Aleksandar
AU  - Ćupina, Branko
AU  - Antanasović, Svetlana
AU  - Krstić, Đorđe
AU  - Karagić, Đura
AU  - Hristov, Nikola
AU  - Kondić-Špika, Ankica
AU  - Kobiljski, Borislav
AU  - Denčić, Srbislav
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3034
AB  - Intercropping annual legumes such as pea (Pisum sativum L.) and vetches (Vicia spp.) is one of the most ancient cultivation practice throughout the world, especially in temperate regions of Europe, Asia Minor, Near and Central East and North Africa. This kind of intercropping may serve for both forage and grain production. In Serbia and throughout the Balkan countries, intercropping annual legumes with cereals is used mostly for providing a high quality fodder in feeding ruminants, especially dairy cows. In a small-plot trial, carried out during the trials years of 2009/2010, 2010/2011 and 2011/2012, there were included the intercrops of eight autumn-sown cereals, namely einkorn (Triticum monococcum L.), emmer (Triticum turgidum L. subsp. dicoccon (Schrank) Thell.), spelt (Triticum aestivum L. subsp. spelta (L.) Thell.), durum wheat (Triticum turgidum L. subsp. durum (Desf.) Husn.), common wheat (Triticum aestivum L. subsp. aestivum), barley (Hordeum vulgare L.), oat (Avena sativa L.) and triticale (×Triticosecale spp.), seven spring-sown annual legumes, such as pea, common vetch (V. sativa L.), Hungarian vetch (V. pannonica Crantz), hairy vetch (V. villosa Roth), faba bean (V.faba L.), grass pea (Lathyrus sativus L.) and white lupin (Lupinus albus L.), and the sole crops of each intercrop component. Each intercrop was sown at a rate of 75%n of a legume and 25% of a cereal in comparison to the sowing rates in their sole crops.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Intercropping autumn-sown annual legumes with cereals for forage production
EP  - 258
SP  - 258
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3034
ER  - 

@conference{
author = "Mihailović, Vojislav and Mikić, Aleksandar and Ćupina, Branko and Antanasović, Svetlana and Krstić, Đorđe and Karagić, Đura and Hristov, Nikola and Kondić-Špika, Ankica and Kobiljski, Borislav and Denčić, Srbislav",
year = "2013",
abstract = "Intercropping annual legumes such as pea (Pisum sativum L.) and vetches (Vicia spp.) is one of the most ancient cultivation practice throughout the world, especially in temperate regions of Europe, Asia Minor, Near and Central East and North Africa. This kind of intercropping may serve for both forage and grain production. In Serbia and throughout the Balkan countries, intercropping annual legumes with cereals is used mostly for providing a high quality fodder in feeding ruminants, especially dairy cows. In a small-plot trial, carried out during the trials years of 2009/2010, 2010/2011 and 2011/2012, there were included the intercrops of eight autumn-sown cereals, namely einkorn (Triticum monococcum L.), emmer (Triticum turgidum L. subsp. dicoccon (Schrank) Thell.), spelt (Triticum aestivum L. subsp. spelta (L.) Thell.), durum wheat (Triticum turgidum L. subsp. durum (Desf.) Husn.), common wheat (Triticum aestivum L. subsp. aestivum), barley (Hordeum vulgare L.), oat (Avena sativa L.) and triticale (×Triticosecale spp.), seven spring-sown annual legumes, such as pea, common vetch (V. sativa L.), Hungarian vetch (V. pannonica Crantz), hairy vetch (V. villosa Roth), faba bean (V.faba L.), grass pea (Lathyrus sativus L.) and white lupin (Lupinus albus L.), and the sole crops of each intercrop component. Each intercrop was sown at a rate of 75%n of a legume and 25% of a cereal in comparison to the sowing rates in their sole crops.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Intercropping autumn-sown annual legumes with cereals for forage production",
pages = "258-258",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3034"
}

Mihailović, V., Mikić, A., Ćupina, B., Antanasović, S., Krstić, Đ., Karagić, Đ., Hristov, N., Kondić-Špika, A., Kobiljski, B.,& Denčić, S.. (2013). Intercropping autumn-sown annual legumes with cereals for forage production. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 258-258.
https://hdl.handle.net/21.15107/rcub_fiver_3034

Mihailović V, Mikić A, Ćupina B, Antanasović S, Krstić Đ, Karagić Đ, Hristov N, Kondić-Špika A, Kobiljski B, Denčić S. Intercropping autumn-sown annual legumes with cereals for forage production. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:258-258.
https://hdl.handle.net/21.15107/rcub_fiver_3034 .

Mihailović, Vojislav, Mikić, Aleksandar, Ćupina, Branko, Antanasović, Svetlana, Krstić, Đorđe, Karagić, Đura, Hristov, Nikola, Kondić-Špika, Ankica, Kobiljski, Borislav, Denčić, Srbislav, "Intercropping autumn-sown annual legumes with cereals for forage production" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):258-258,
https://hdl.handle.net/21.15107/rcub_fiver_3034 .

TY  - JOUR
AU  - Brdar-Jokanović, Milka
AU  - Maksimović, Ivana
AU  - Kraljević-Balalić, Marija
AU  - Zeremski-Škorić, Tijana
AU  - Kondić-Špika, Ankica
AU  - Kobiljski, Borislav
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1262
AB  - The objective of this study was to investigate the relationships among boron (B) concentration, boron content, and tolerance to excess boron in 40 wheat (Triticum aestivum L.) genotypes. Boron tolerance was estimated at seedling stage by filter paper technique, imposing three boron treatments (50, 100 and 150 mg L-1 boric acid, H3BO3) and comparing the response of root length and dry weight to the control (0.93 mg L-1 H3BO3). Although substantial variation in boron tolerance, concentration, and content has been found among the studied wheat genotypes, regression analysis showed no relationship between neither root length reduction and boron concentration, nor dry weight reduction and boron concentration. On the other hand, a positive linear relationship was found between boron content and both root length and dry weight response to high external boron; tolerant wheat genotypes were characterized by higher boron content than the sensitive ones. The results may be explained by significant decline of root length and dry weight in sensitive genotypes, which caused reduced uptake of all nutrients, including boron. Vice versa, root length and dry weight of tolerant genotypes were affected by boron treatments to the smaller extent, allowing the uptake of higher amounts of boron and resulting in comparatively high boron content.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Plant Nutrition
T1  - Boron concentration vs. Content as criterion for estimating boron tolerance in wheat
EP  - 480
IS  - 3
SP  - 470
VL  - 36
DO  - 10.1080/01904167.2012.748067
ER  - 

@article{
author = "Brdar-Jokanović, Milka and Maksimović, Ivana and Kraljević-Balalić, Marija and Zeremski-Škorić, Tijana and Kondić-Špika, Ankica and Kobiljski, Borislav",
year = "2013",
abstract = "The objective of this study was to investigate the relationships among boron (B) concentration, boron content, and tolerance to excess boron in 40 wheat (Triticum aestivum L.) genotypes. Boron tolerance was estimated at seedling stage by filter paper technique, imposing three boron treatments (50, 100 and 150 mg L-1 boric acid, H3BO3) and comparing the response of root length and dry weight to the control (0.93 mg L-1 H3BO3). Although substantial variation in boron tolerance, concentration, and content has been found among the studied wheat genotypes, regression analysis showed no relationship between neither root length reduction and boron concentration, nor dry weight reduction and boron concentration. On the other hand, a positive linear relationship was found between boron content and both root length and dry weight response to high external boron; tolerant wheat genotypes were characterized by higher boron content than the sensitive ones. The results may be explained by significant decline of root length and dry weight in sensitive genotypes, which caused reduced uptake of all nutrients, including boron. Vice versa, root length and dry weight of tolerant genotypes were affected by boron treatments to the smaller extent, allowing the uptake of higher amounts of boron and resulting in comparatively high boron content.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Plant Nutrition",
title = "Boron concentration vs. Content as criterion for estimating boron tolerance in wheat",
pages = "480-470",
number = "3",
volume = "36",
doi = "10.1080/01904167.2012.748067"
}

Brdar-Jokanović, M., Maksimović, I., Kraljević-Balalić, M., Zeremski-Škorić, T., Kondić-Špika, A.,& Kobiljski, B.. (2013). Boron concentration vs. Content as criterion for estimating boron tolerance in wheat. in Journal of Plant Nutrition
Taylor & Francis Inc, Philadelphia., 36(3), 470-480.
https://doi.org/10.1080/01904167.2012.748067

Brdar-Jokanović M, Maksimović I, Kraljević-Balalić M, Zeremski-Škorić T, Kondić-Špika A, Kobiljski B. Boron concentration vs. Content as criterion for estimating boron tolerance in wheat. in Journal of Plant Nutrition. 2013;36(3):470-480.
doi:10.1080/01904167.2012.748067 .

Brdar-Jokanović, Milka, Maksimović, Ivana, Kraljević-Balalić, Marija, Zeremski-Škorić, Tijana, Kondić-Špika, Ankica, Kobiljski, Borislav, "Boron concentration vs. Content as criterion for estimating boron tolerance in wheat" in Journal of Plant Nutrition, 36, no. 3 (2013):470-480,
https://doi.org/10.1080/01904167.2012.748067 . .

TY  - JOUR
AU  - Jones, H.
AU  - Gosman, N.
AU  - Horsnell, R.
AU  - Rose, G. A.
AU  - Everest, L. A.
AU  - Bentley, A. R.
AU  - Tha, S.
AU  - Uauy, C.
AU  - Kowalski, A.
AU  - Novoselović, Dario
AU  - Simek, R.
AU  - Kobiljski, Borislav
AU  - Kondić-Špika, Ankica
AU  - Brbaklić, Ljiljana
AU  - Mitrofanova, O.
AU  - Chesnokov, Y.
AU  - Bonnett, D.
AU  - Greenland, A.
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1255
AB  - Hexaploid bread wheat evolved from a rare hybridisation, which resulted in a loss of genetic diversity in the wheat D-genome with respect to the ancestral donor, Aegilops tauschii. Novel genetic variation can be introduced into modern wheat by recreating the above hybridisation; however, the information associated with the Ae. tauschii accessions in germplasm collections is limited, making rational selection of accessions into a re-synthesis programme difficult. We describe methodologies to identify novel diversity from Ae. tauschii accessions that combines Bayesian analysis of genotypic data, sub-species diversity and geographic information that summarises variation in climate and habitat at the collection point for each accession. Comparisons were made between diversity discovered amongst a panel of Ae. tauschii accessions, bread wheat varieties and lines from the CIMMYT synthetic hexaploid wheat programme. The selection of Ae. tauschii accessions based on differing approaches had significant effect on diversity within each set. Our results suggest that a strategy that combines several criteria will be most effective in maximising the sampled variation across multiple parameters. The analysis of multiple layers of variation in ex situ Ae. tauschii collections allows for an informed and rational approach to the inclusion of wild relatives into crop breeding programmes.
PB  - Springer, New York
T2  - Theoretical & Applied Genetics
T1  - Strategy for exploiting exotic germplasm using genetic, morphological, and environmental diversity: the Aegilops tauschii Coss. example
EP  - 1808
IS  - 7
SP  - 1793
VL  - 126
DO  - 10.1007/s00122-013-2093-x
ER  - 

@article{
author = "Jones, H. and Gosman, N. and Horsnell, R. and Rose, G. A. and Everest, L. A. and Bentley, A. R. and Tha, S. and Uauy, C. and Kowalski, A. and Novoselović, Dario and Simek, R. and Kobiljski, Borislav and Kondić-Špika, Ankica and Brbaklić, Ljiljana and Mitrofanova, O. and Chesnokov, Y. and Bonnett, D. and Greenland, A.",
year = "2013",
abstract = "Hexaploid bread wheat evolved from a rare hybridisation, which resulted in a loss of genetic diversity in the wheat D-genome with respect to the ancestral donor, Aegilops tauschii. Novel genetic variation can be introduced into modern wheat by recreating the above hybridisation; however, the information associated with the Ae. tauschii accessions in germplasm collections is limited, making rational selection of accessions into a re-synthesis programme difficult. We describe methodologies to identify novel diversity from Ae. tauschii accessions that combines Bayesian analysis of genotypic data, sub-species diversity and geographic information that summarises variation in climate and habitat at the collection point for each accession. Comparisons were made between diversity discovered amongst a panel of Ae. tauschii accessions, bread wheat varieties and lines from the CIMMYT synthetic hexaploid wheat programme. The selection of Ae. tauschii accessions based on differing approaches had significant effect on diversity within each set. Our results suggest that a strategy that combines several criteria will be most effective in maximising the sampled variation across multiple parameters. The analysis of multiple layers of variation in ex situ Ae. tauschii collections allows for an informed and rational approach to the inclusion of wild relatives into crop breeding programmes.",
publisher = "Springer, New York",
journal = "Theoretical & Applied Genetics",
title = "Strategy for exploiting exotic germplasm using genetic, morphological, and environmental diversity: the Aegilops tauschii Coss. example",
pages = "1808-1793",
number = "7",
volume = "126",
doi = "10.1007/s00122-013-2093-x"
}

Jones, H., Gosman, N., Horsnell, R., Rose, G. A., Everest, L. A., Bentley, A. R., Tha, S., Uauy, C., Kowalski, A., Novoselović, D., Simek, R., Kobiljski, B., Kondić-Špika, A., Brbaklić, L., Mitrofanova, O., Chesnokov, Y., Bonnett, D.,& Greenland, A.. (2013). Strategy for exploiting exotic germplasm using genetic, morphological, and environmental diversity: the Aegilops tauschii Coss. example. in Theoretical & Applied Genetics
Springer, New York., 126(7), 1793-1808.
https://doi.org/10.1007/s00122-013-2093-x

Jones H, Gosman N, Horsnell R, Rose GA, Everest LA, Bentley AR, Tha S, Uauy C, Kowalski A, Novoselović D, Simek R, Kobiljski B, Kondić-Špika A, Brbaklić L, Mitrofanova O, Chesnokov Y, Bonnett D, Greenland A. Strategy for exploiting exotic germplasm using genetic, morphological, and environmental diversity: the Aegilops tauschii Coss. example. in Theoretical & Applied Genetics. 2013;126(7):1793-1808.
doi:10.1007/s00122-013-2093-x .

Jones, H., Gosman, N., Horsnell, R., Rose, G. A., Everest, L. A., Bentley, A. R., Tha, S., Uauy, C., Kowalski, A., Novoselović, Dario, Simek, R., Kobiljski, Borislav, Kondić-Špika, Ankica, Brbaklić, Ljiljana, Mitrofanova, O., Chesnokov, Y., Bonnett, D., Greenland, A., "Strategy for exploiting exotic germplasm using genetic, morphological, and environmental diversity: the Aegilops tauschii Coss. example" in Theoretical & Applied Genetics, 126, no. 7 (2013):1793-1808,
https://doi.org/10.1007/s00122-013-2093-x . .

TY  - JOUR
AU  - Brbaklić, Ljiljana
AU  - Trkulja, Dragana
AU  - Kondić-Špika, Ankica
AU  - Treskić, Sanja
AU  - Kobiljski, Borislav
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1243
AB  - BRBAKLIC L., TRKULJA D., KONDIC-SPIKA A., TRESKIC S., KOBILJSKI B. (2013): Detection of QTLs for important agronomical traits in hexaploid wheat using association analysis. Czech J. Genet. Plant Breed., 49: 1-8. One of the main wheat breeder's goals is determining specific genomic regions which control important agronomical traits. Association analysis is a new strategy with high resolution in plant molecular breeding that could be used to improve the efficiency of marker assisted selection (MAS) for finding important QTLs (quantitative trait loci) or genes. A set of 96 diverse wheat genotypes was phenotypically measured during three growing seasons (2006/07, 2007/08, 2008/09). Microsatellite markers located near important QTLs were carefully chosen in accordance with existing literature data to validate marker trait associations (MTA). Genomic DNA was extracted using the CTAB method and PCR products were separated by capillary electrophoresis. The population structure was assigned based on molecular data in Structure v. 2.0 software, while association analysis was done by the Tassel program using the Q matrix. Nine significant associations were stable in all years investigated and eight MTA were detected to be significant in two growing seasons. Microsatellite markers which showed significant associations and stability in different seasons can be useful and suitable for marker assisted selection (MAS) in Serbian wheat breeding programs.
PB  - Czech Academy Agricultural Sciences, Prague
T2  - Czech Journal of Genetics & Plant Breeding
T1  - Detection of QTLs for Important Agronomical Traits in Hexaploid Wheat Using Association Analysis
EP  - 8
IS  - 1
SP  - 1
VL  - 49
DO  - 10.17221/64/2012-CJGPB
ER  - 

@article{
author = "Brbaklić, Ljiljana and Trkulja, Dragana and Kondić-Špika, Ankica and Treskić, Sanja and Kobiljski, Borislav",
year = "2013",
abstract = "BRBAKLIC L., TRKULJA D., KONDIC-SPIKA A., TRESKIC S., KOBILJSKI B. (2013): Detection of QTLs for important agronomical traits in hexaploid wheat using association analysis. Czech J. Genet. Plant Breed., 49: 1-8. One of the main wheat breeder's goals is determining specific genomic regions which control important agronomical traits. Association analysis is a new strategy with high resolution in plant molecular breeding that could be used to improve the efficiency of marker assisted selection (MAS) for finding important QTLs (quantitative trait loci) or genes. A set of 96 diverse wheat genotypes was phenotypically measured during three growing seasons (2006/07, 2007/08, 2008/09). Microsatellite markers located near important QTLs were carefully chosen in accordance with existing literature data to validate marker trait associations (MTA). Genomic DNA was extracted using the CTAB method and PCR products were separated by capillary electrophoresis. The population structure was assigned based on molecular data in Structure v. 2.0 software, while association analysis was done by the Tassel program using the Q matrix. Nine significant associations were stable in all years investigated and eight MTA were detected to be significant in two growing seasons. Microsatellite markers which showed significant associations and stability in different seasons can be useful and suitable for marker assisted selection (MAS) in Serbian wheat breeding programs.",
publisher = "Czech Academy Agricultural Sciences, Prague",
journal = "Czech Journal of Genetics & Plant Breeding",
title = "Detection of QTLs for Important Agronomical Traits in Hexaploid Wheat Using Association Analysis",
pages = "8-1",
number = "1",
volume = "49",
doi = "10.17221/64/2012-CJGPB"
}

Brbaklić, L., Trkulja, D., Kondić-Špika, A., Treskić, S.,& Kobiljski, B.. (2013). Detection of QTLs for Important Agronomical Traits in Hexaploid Wheat Using Association Analysis. in Czech Journal of Genetics & Plant Breeding
Czech Academy Agricultural Sciences, Prague., 49(1), 1-8.
https://doi.org/10.17221/64/2012-CJGPB

Brbaklić L, Trkulja D, Kondić-Špika A, Treskić S, Kobiljski B. Detection of QTLs for Important Agronomical Traits in Hexaploid Wheat Using Association Analysis. in Czech Journal of Genetics & Plant Breeding. 2013;49(1):1-8.
doi:10.17221/64/2012-CJGPB .

Brbaklić, Ljiljana, Trkulja, Dragana, Kondić-Špika, Ankica, Treskić, Sanja, Kobiljski, Borislav, "Detection of QTLs for Important Agronomical Traits in Hexaploid Wheat Using Association Analysis" in Czech Journal of Genetics & Plant Breeding, 49, no. 1 (2013):1-8,
https://doi.org/10.17221/64/2012-CJGPB . .

TY  - JOUR
AU  - Brdar-Jokanović, Milka
AU  - Trkulja, Dragana
AU  - Nikolić-Đorić, Emilija
AU  - Kondić-Špika, Ankica
AU  - Kobiljski, Borislav
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1193
AB  - The aim of this study was to investigate the possibilities of applying molecular markers-microsatellites in breeding boron tolerant wheat. The study comprised the investigation of allelic variability of sixty bread wheat accessions in two microsatellite loci (Xgwm46-7B and Xgwm577-7B) for which was assumed that are placed near the 7B chromosome locus involved in the expression of boron tolerance in wheat. Phenotypic variability concerning boron tolerance was assessed via root length reduction of wheat seedlings grown in the presence of high external boron, applied as boric acid solution (concentrations 50, 100 and 150 mg/l, boron treatments B50, B100 and B150). The indication of marker-trait associations was determined by comparing the allelic variability in the two microsatellite loci with the phenotypic variability in boron tolerance. Nonparametric Kruskal-Wallis test was used for the comparisons. The indication of marker-trait association was found for both Xgwm46-7B and Xgwm577-7B; on B150 and B50 treatments, respectively. Allelic forms identified in Xgwm577-7B locus may be related to tolerance, medium tolerance and sensitivity to high boron. This was not the case for Xgwm46-7B, where the identified alleles were related only to boron tolerance and sensitivity. Therefore, Xgwm577-7B may be preferred over Xgwm46-7B when studying boron tolerance in wheat. However, a considerable portion of boron tolerant accessions carried different alleles in the investigated loci, implying boron tolerance as a quantitative trait with more than one chromosomal region involved in its expression. Therefore, the allelic variability of more than the analyzed two loci should be investigated.
AB  - Cilj ovog rada je bilo ispitivanje mogućnosti primene molekularnih markera-mikrosatelita u oplemenjivanju pšenice tolerantne na bor. Ispitana je alelna varijabilnost kod šezdeset genotipova hlebne pšenice u dva mikrosatelitna lokusa (Xgwm46-7B i Xgwm577-7B) za koje se pretpostavlja da su pozicionirani u blizini lokusa na 7B hromozomu koji utiče na ekspresiju ovog svojstva. Fenotipska varijabilnost u pogledu tolerantnosti na bor je utvrđena na osnovu redukcije dužine korenčića klijanaca pšenice gajenih u uslovima suviška bora (rastvor borne kiseline koncentracija 50, 100 i 150 mg/l, tretmani B50, B100, B150). Indikacija veze marker-svojstvo je utvrđivana na osnovu poređenja alelne varijabilnosti mikrosatelitnih lokusa i fenotipske varijabilnosti u pogledu tolerantnosti na bor. Za poređenja je korišćen neparametarski Kruskal-Wallis test. Indikacija veze marker-svojstvo je konstatovana za Xgwm46-7B na tretmanu B150, a za Xgwm577-7B na tretmanu B50. Alelne forme identifikovane u lokusu Xgwm577-7B se mogu dovesti u vezu sa tolerantnošću, srednjom tolerantnošću i osetljivošću na bor. Ovo nije bio slučaj kod Xgwm46-7B, gde su identifikovani aleli dovedeni u vezu samo sa tolerantnošću i osetljivošću na bor. Prema tome, za dalji rad se preporučuje Xgwm577-7B. Međutim, kod značajnog dela tolerantnih genotipova su identifikovani različiti aleli u proučavanim lokusima, što upućuje na zaključak da je u pitanju kvantitativno svojstvo u čiju ekspresiju je uključeno više od jednog hromozomskog regiona. Potrebno je ispitati alelnu varijabilnost više od dva mikrosatelitna lokusa.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - The possibilities of applying marker assisted selection in breeding boron tolerant wheat
T1  - Mogućnosti primene marker-asistirane selekcije u oplemenjivanju pšenice tolerantne na bor
EP  - 776
IS  - 3
SP  - 769
VL  - 45
DO  - 10.2298/GENSR1303769J
ER  - 

@article{
author = "Brdar-Jokanović, Milka and Trkulja, Dragana and Nikolić-Đorić, Emilija and Kondić-Špika, Ankica and Kobiljski, Borislav",
year = "2013",
abstract = "The aim of this study was to investigate the possibilities of applying molecular markers-microsatellites in breeding boron tolerant wheat. The study comprised the investigation of allelic variability of sixty bread wheat accessions in two microsatellite loci (Xgwm46-7B and Xgwm577-7B) for which was assumed that are placed near the 7B chromosome locus involved in the expression of boron tolerance in wheat. Phenotypic variability concerning boron tolerance was assessed via root length reduction of wheat seedlings grown in the presence of high external boron, applied as boric acid solution (concentrations 50, 100 and 150 mg/l, boron treatments B50, B100 and B150). The indication of marker-trait associations was determined by comparing the allelic variability in the two microsatellite loci with the phenotypic variability in boron tolerance. Nonparametric Kruskal-Wallis test was used for the comparisons. The indication of marker-trait association was found for both Xgwm46-7B and Xgwm577-7B; on B150 and B50 treatments, respectively. Allelic forms identified in Xgwm577-7B locus may be related to tolerance, medium tolerance and sensitivity to high boron. This was not the case for Xgwm46-7B, where the identified alleles were related only to boron tolerance and sensitivity. Therefore, Xgwm577-7B may be preferred over Xgwm46-7B when studying boron tolerance in wheat. However, a considerable portion of boron tolerant accessions carried different alleles in the investigated loci, implying boron tolerance as a quantitative trait with more than one chromosomal region involved in its expression. Therefore, the allelic variability of more than the analyzed two loci should be investigated., Cilj ovog rada je bilo ispitivanje mogućnosti primene molekularnih markera-mikrosatelita u oplemenjivanju pšenice tolerantne na bor. Ispitana je alelna varijabilnost kod šezdeset genotipova hlebne pšenice u dva mikrosatelitna lokusa (Xgwm46-7B i Xgwm577-7B) za koje se pretpostavlja da su pozicionirani u blizini lokusa na 7B hromozomu koji utiče na ekspresiju ovog svojstva. Fenotipska varijabilnost u pogledu tolerantnosti na bor je utvrđena na osnovu redukcije dužine korenčića klijanaca pšenice gajenih u uslovima suviška bora (rastvor borne kiseline koncentracija 50, 100 i 150 mg/l, tretmani B50, B100, B150). Indikacija veze marker-svojstvo je utvrđivana na osnovu poređenja alelne varijabilnosti mikrosatelitnih lokusa i fenotipske varijabilnosti u pogledu tolerantnosti na bor. Za poređenja je korišćen neparametarski Kruskal-Wallis test. Indikacija veze marker-svojstvo je konstatovana za Xgwm46-7B na tretmanu B150, a za Xgwm577-7B na tretmanu B50. Alelne forme identifikovane u lokusu Xgwm577-7B se mogu dovesti u vezu sa tolerantnošću, srednjom tolerantnošću i osetljivošću na bor. Ovo nije bio slučaj kod Xgwm46-7B, gde su identifikovani aleli dovedeni u vezu samo sa tolerantnošću i osetljivošću na bor. Prema tome, za dalji rad se preporučuje Xgwm577-7B. Međutim, kod značajnog dela tolerantnih genotipova su identifikovani različiti aleli u proučavanim lokusima, što upućuje na zaključak da je u pitanju kvantitativno svojstvo u čiju ekspresiju je uključeno više od jednog hromozomskog regiona. Potrebno je ispitati alelnu varijabilnost više od dva mikrosatelitna lokusa.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "The possibilities of applying marker assisted selection in breeding boron tolerant wheat, Mogućnosti primene marker-asistirane selekcije u oplemenjivanju pšenice tolerantne na bor",
pages = "776-769",
number = "3",
volume = "45",
doi = "10.2298/GENSR1303769J"
}

Brdar-Jokanović, M., Trkulja, D., Nikolić-Đorić, E., Kondić-Špika, A.,& Kobiljski, B.. (2013). The possibilities of applying marker assisted selection in breeding boron tolerant wheat. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 45(3), 769-776.
https://doi.org/10.2298/GENSR1303769J

Brdar-Jokanović M, Trkulja D, Nikolić-Đorić E, Kondić-Špika A, Kobiljski B. The possibilities of applying marker assisted selection in breeding boron tolerant wheat. in Genetika-Belgrade. 2013;45(3):769-776.
doi:10.2298/GENSR1303769J .

Brdar-Jokanović, Milka, Trkulja, Dragana, Nikolić-Đorić, Emilija, Kondić-Špika, Ankica, Kobiljski, Borislav, "The possibilities of applying marker assisted selection in breeding boron tolerant wheat" in Genetika-Belgrade, 45, no. 3 (2013):769-776,
https://doi.org/10.2298/GENSR1303769J . .

TY  - JOUR
AU  - Denčić, Srbislav
AU  - DePauw, Ron
AU  - Kobiljski, Borislav
AU  - Momčilović, Vojislava
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1191
AB  - The effects of dry and wet preharvest periods on Hagberg falling number (HFN), a parameter of alpha-amylase activity, and rheological properties including farinograph dough development time (FDT), farinograph absorption (FA), resistance to extension (RE), loaf volume (LV) and baking score (BS) were examined in 30 hexaploid wheat (Triticum aestivum L.) cultivars originating from 19 countries. The cultivars were grown in the field in 2000 - 2010 and HEN and theological properties were analyzed for three replicates. The cultivars were divided into three groups according to BIN in the wet preharvest period: HFN below 150 s (group H-1), HEN from 250 to 350 s (group H-2), and BIN over 400 s (group H-3). The cultivars in group H-3 were superior to those in either group H-1 or H-2 in all rheological properties except RE. In the dry preharvest periods, HFN was not correlated with rheological properties, while in the wet preharvest period HEN showed a highly significant positive correlation with FDT and BS. The canonical variate analysis for assessment of the general performance of all cultivars with HFN as the main factor and the other rheological properties as subfactors, indicated that the cultivars Stepnaja 30, Garazinko, Kirac, Klein Forten and Zitarka showed the highest potential regardless of preharvest rainfall amounts. In conclusion, differential genetic expression of resistance to preharvest sprouting, maintenance of low alpha amylase level, high HEN values, maintenance of theological properties, and baking performance can be reliably detected and measured under wet preharvest conditions.
PB  - Crop Science Soc Japan, Tokyo
T2  - Plant Production Science
T1  - Hagberg Falling Number and Rheological Properties of Wheat Cultivars in Wet and Dry Preharvest Periods
EP  - 351
IS  - 4
SP  - 342
VL  - 16
DO  - 10.1626/pps.16.342
ER  - 

@article{
author = "Denčić, Srbislav and DePauw, Ron and Kobiljski, Borislav and Momčilović, Vojislava",
year = "2013",
abstract = "The effects of dry and wet preharvest periods on Hagberg falling number (HFN), a parameter of alpha-amylase activity, and rheological properties including farinograph dough development time (FDT), farinograph absorption (FA), resistance to extension (RE), loaf volume (LV) and baking score (BS) were examined in 30 hexaploid wheat (Triticum aestivum L.) cultivars originating from 19 countries. The cultivars were grown in the field in 2000 - 2010 and HEN and theological properties were analyzed for three replicates. The cultivars were divided into three groups according to BIN in the wet preharvest period: HFN below 150 s (group H-1), HEN from 250 to 350 s (group H-2), and BIN over 400 s (group H-3). The cultivars in group H-3 were superior to those in either group H-1 or H-2 in all rheological properties except RE. In the dry preharvest periods, HFN was not correlated with rheological properties, while in the wet preharvest period HEN showed a highly significant positive correlation with FDT and BS. The canonical variate analysis for assessment of the general performance of all cultivars with HFN as the main factor and the other rheological properties as subfactors, indicated that the cultivars Stepnaja 30, Garazinko, Kirac, Klein Forten and Zitarka showed the highest potential regardless of preharvest rainfall amounts. In conclusion, differential genetic expression of resistance to preharvest sprouting, maintenance of low alpha amylase level, high HEN values, maintenance of theological properties, and baking performance can be reliably detected and measured under wet preharvest conditions.",
publisher = "Crop Science Soc Japan, Tokyo",
journal = "Plant Production Science",
title = "Hagberg Falling Number and Rheological Properties of Wheat Cultivars in Wet and Dry Preharvest Periods",
pages = "351-342",
number = "4",
volume = "16",
doi = "10.1626/pps.16.342"
}

Denčić, S., DePauw, R., Kobiljski, B.,& Momčilović, V.. (2013). Hagberg Falling Number and Rheological Properties of Wheat Cultivars in Wet and Dry Preharvest Periods. in Plant Production Science
Crop Science Soc Japan, Tokyo., 16(4), 342-351.
https://doi.org/10.1626/pps.16.342

Denčić S, DePauw R, Kobiljski B, Momčilović V. Hagberg Falling Number and Rheological Properties of Wheat Cultivars in Wet and Dry Preharvest Periods. in Plant Production Science. 2013;16(4):342-351.
doi:10.1626/pps.16.342 .

Denčić, Srbislav, DePauw, Ron, Kobiljski, Borislav, Momčilović, Vojislava, "Hagberg Falling Number and Rheological Properties of Wheat Cultivars in Wet and Dry Preharvest Periods" in Plant Production Science, 16, no. 4 (2013):342-351,
https://doi.org/10.1626/pps.16.342 . .

TY  - CONF
AU  - Kondić-Špika, Ankica
AU  - Brbaklić, Ljiljana
AU  - Trkulja, Dragana
AU  - Kobiljski, Borislav
AU  - Đurić, Veselinka
AU  - Mladenov, Novica
AU  - Hristov, Nikola
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1185
AB  - Wheat quality is a complex collection of traits affected by various loci on multiple chromosomes. This study was conducted in order to identify quantitative trait loci (QTLs) related to most commonly used quality parameters, protein content (PC) and sedimentation value (SED). For this purpose association analysis between SSR molecular markers and quality traits was performed on a selected sample of 94 diverse wheat genotypes. Quality parameters were determined during five years (2007-2011). Microsatellite markers located near targeted QTLs were carefully chosen in accordance with existing literature data. Genomic DNA was extracted from seedlings using CTAB method and PCR products were separated by capillary electrophoresis. The population structure was estimated in Structure v.2.0. software and association analysis was performed in the program Tassel. Significant marker-trait associations (MTAs) were found for the both quality parameters. The markers WMC31 and GWM18 showed significant and stabile associations with QTLs for PC and SED, respectively. Our results demonstrated that association analysis could complement and enhance previous QTL information and provide very useful information for marker-assisted selection.
PB  - University of Novi Sad, Faculty of Technology
C3  - CEFood 2012 - Proceedings of 6th Central European Congress on Food
T1  - Associations between microsatellite markers and wheat quality parameters
EP  - 407
SP  - 403
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1185
ER  - 

@conference{
author = "Kondić-Špika, Ankica and Brbaklić, Ljiljana and Trkulja, Dragana and Kobiljski, Borislav and Đurić, Veselinka and Mladenov, Novica and Hristov, Nikola",
year = "2012",
abstract = "Wheat quality is a complex collection of traits affected by various loci on multiple chromosomes. This study was conducted in order to identify quantitative trait loci (QTLs) related to most commonly used quality parameters, protein content (PC) and sedimentation value (SED). For this purpose association analysis between SSR molecular markers and quality traits was performed on a selected sample of 94 diverse wheat genotypes. Quality parameters were determined during five years (2007-2011). Microsatellite markers located near targeted QTLs were carefully chosen in accordance with existing literature data. Genomic DNA was extracted from seedlings using CTAB method and PCR products were separated by capillary electrophoresis. The population structure was estimated in Structure v.2.0. software and association analysis was performed in the program Tassel. Significant marker-trait associations (MTAs) were found for the both quality parameters. The markers WMC31 and GWM18 showed significant and stabile associations with QTLs for PC and SED, respectively. Our results demonstrated that association analysis could complement and enhance previous QTL information and provide very useful information for marker-assisted selection.",
publisher = "University of Novi Sad, Faculty of Technology",
journal = "CEFood 2012 - Proceedings of 6th Central European Congress on Food",
title = "Associations between microsatellite markers and wheat quality parameters",
pages = "407-403",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1185"
}

Kondić-Špika, A., Brbaklić, L., Trkulja, D., Kobiljski, B., Đurić, V., Mladenov, N.,& Hristov, N.. (2012). Associations between microsatellite markers and wheat quality parameters. in CEFood 2012 - Proceedings of 6th Central European Congress on Food
University of Novi Sad, Faculty of Technology., 403-407.
https://hdl.handle.net/21.15107/rcub_fiver_1185

Kondić-Špika A, Brbaklić L, Trkulja D, Kobiljski B, Đurić V, Mladenov N, Hristov N. Associations between microsatellite markers and wheat quality parameters. in CEFood 2012 - Proceedings of 6th Central European Congress on Food. 2012;:403-407.
https://hdl.handle.net/21.15107/rcub_fiver_1185 .

Kondić-Špika, Ankica, Brbaklić, Ljiljana, Trkulja, Dragana, Kobiljski, Borislav, Đurić, Veselinka, Mladenov, Novica, Hristov, Nikola, "Associations between microsatellite markers and wheat quality parameters" in CEFood 2012 - Proceedings of 6th Central European Congress on Food (2012):403-407,
https://hdl.handle.net/21.15107/rcub_fiver_1185 .

TY  - JOUR
AU  - Dodig, Dejan
AU  - Zorić, Miroslav
AU  - Kobiljski, Borislav
AU  - Savić, Jasna
AU  - Kandić, Vesna
AU  - Quarrie, Steve
AU  - Barnes, Jeremy
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1182
AB  - Genetic analyses and association mapping were performed on a winter wheat core collection of 96 accessions sampled from a variety of geographic origins. Twenty-four agronomic traits were evaluated over 3 years under fully irrigated, rainfed and drought treatments. Grain yield was the most sensitive trait to water deficit and was highly correlated with above-ground biomass per plant and number of kernels per m2. The germplasm was structured into four subpopulations. The association of 46 SSR loci distributed throughout the wheat genome with yield and agronomic traits was analyzed using a general linear model, where subpopulation information was used to control false-positive or spurious marker-trait associations (MTAs). A total of 26, 21 and 29 significant (P < 0.001) MTAs were identified in irrigated, rainfed and drought treatments, respectively. The marker effects ranged from 14.0 to 50.8%. Combined across all treatments, 34 significant (P < 0.001) MTAs were identified with nine markers, and R2 ranged from 14.5 to 50.2%. Marker psp3200 (6DS) and particularly gwm484 (2DS) were associated with many significant MTAs in each treatment and explained the greatest proportion of phenotypic variation. Although we were not able to recognize any marker related to grain yield under drought stress, a number of MTAs associated with developmental and agronomic traits highly correlated with grain yield under drought were identified.
T2  - International Journal of Molecular Sciences
T1  - Genetic and association mapping study of wheat agronomic traits under contrasting water regimes
EP  - 6188
IS  - 5
SP  - 6167
VL  - 13
DO  - 10.3390/ijms13056167
ER  - 

@article{
author = "Dodig, Dejan and Zorić, Miroslav and Kobiljski, Borislav and Savić, Jasna and Kandić, Vesna and Quarrie, Steve and Barnes, Jeremy",
year = "2012",
abstract = "Genetic analyses and association mapping were performed on a winter wheat core collection of 96 accessions sampled from a variety of geographic origins. Twenty-four agronomic traits were evaluated over 3 years under fully irrigated, rainfed and drought treatments. Grain yield was the most sensitive trait to water deficit and was highly correlated with above-ground biomass per plant and number of kernels per m2. The germplasm was structured into four subpopulations. The association of 46 SSR loci distributed throughout the wheat genome with yield and agronomic traits was analyzed using a general linear model, where subpopulation information was used to control false-positive or spurious marker-trait associations (MTAs). A total of 26, 21 and 29 significant (P < 0.001) MTAs were identified in irrigated, rainfed and drought treatments, respectively. The marker effects ranged from 14.0 to 50.8%. Combined across all treatments, 34 significant (P < 0.001) MTAs were identified with nine markers, and R2 ranged from 14.5 to 50.2%. Marker psp3200 (6DS) and particularly gwm484 (2DS) were associated with many significant MTAs in each treatment and explained the greatest proportion of phenotypic variation. Although we were not able to recognize any marker related to grain yield under drought stress, a number of MTAs associated with developmental and agronomic traits highly correlated with grain yield under drought were identified.",
journal = "International Journal of Molecular Sciences",
title = "Genetic and association mapping study of wheat agronomic traits under contrasting water regimes",
pages = "6188-6167",
number = "5",
volume = "13",
doi = "10.3390/ijms13056167"
}

Dodig, D., Zorić, M., Kobiljski, B., Savić, J., Kandić, V., Quarrie, S.,& Barnes, J.. (2012). Genetic and association mapping study of wheat agronomic traits under contrasting water regimes. in International Journal of Molecular Sciences, 13(5), 6167-6188.
https://doi.org/10.3390/ijms13056167

Dodig D, Zorić M, Kobiljski B, Savić J, Kandić V, Quarrie S, Barnes J. Genetic and association mapping study of wheat agronomic traits under contrasting water regimes. in International Journal of Molecular Sciences. 2012;13(5):6167-6188.
doi:10.3390/ijms13056167 .

Dodig, Dejan, Zorić, Miroslav, Kobiljski, Borislav, Savić, Jasna, Kandić, Vesna, Quarrie, Steve, Barnes, Jeremy, "Genetic and association mapping study of wheat agronomic traits under contrasting water regimes" in International Journal of Molecular Sciences, 13, no. 5 (2012):6167-6188,
https://doi.org/10.3390/ijms13056167 . .

TY  - JOUR
AU  - Trkulja, Dragana
AU  - Kondić-Špika, Ankica
AU  - Brbaklić, Ljiljana
AU  - Kobiljski, Borislav
AU  - Hristov, Nikola
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1154
AB  - Phenotypic assessment of yield and its component traits needs to be performed in replicated trials under multiple environments. Mapping populations such as doubled haploids (DHs) are suitable material for studying marker-trait associations, because they can be evaluated repeatedly in different years or in variable environments, thus having considerable potential for the identification of reliable quantitative trait loci. In the present study a DH mapping population, generated from the crossing of two hexaploid high yield potential wheat varieties, Savannah (UK) and Renesansa (SRB), was used to identify associations between five microsatellite markers and seven spike-related traits. Statistically significant associations were observed between marker GWM261 on 2DS chromosome and six spike-related traits: spike length, spikelet number per spike, sterile spikelet number per spike, grain weight, spike weight and spike index. The phenotypic variation explained by this marker was the highest for the trait spike length (from 8.3 to 24.7%).
T2  - Romanian Agricultural Research
T1  - Marker-trait associations for spike-related characters in a doubled haploid population of wheat
EP  - 16
IS  - 29
SP  - 9
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1154
ER  - 

@article{
author = "Trkulja, Dragana and Kondić-Špika, Ankica and Brbaklić, Ljiljana and Kobiljski, Borislav and Hristov, Nikola",
year = "2012",
abstract = "Phenotypic assessment of yield and its component traits needs to be performed in replicated trials under multiple environments. Mapping populations such as doubled haploids (DHs) are suitable material for studying marker-trait associations, because they can be evaluated repeatedly in different years or in variable environments, thus having considerable potential for the identification of reliable quantitative trait loci. In the present study a DH mapping population, generated from the crossing of two hexaploid high yield potential wheat varieties, Savannah (UK) and Renesansa (SRB), was used to identify associations between five microsatellite markers and seven spike-related traits. Statistically significant associations were observed between marker GWM261 on 2DS chromosome and six spike-related traits: spike length, spikelet number per spike, sterile spikelet number per spike, grain weight, spike weight and spike index. The phenotypic variation explained by this marker was the highest for the trait spike length (from 8.3 to 24.7%).",
journal = "Romanian Agricultural Research",
title = "Marker-trait associations for spike-related characters in a doubled haploid population of wheat",
pages = "16-9",
number = "29",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1154"
}

Trkulja, D., Kondić-Špika, A., Brbaklić, L., Kobiljski, B.,& Hristov, N.. (2012). Marker-trait associations for spike-related characters in a doubled haploid population of wheat. in Romanian Agricultural Research(29), 9-16.
https://hdl.handle.net/21.15107/rcub_fiver_1154

Trkulja D, Kondić-Špika A, Brbaklić L, Kobiljski B, Hristov N. Marker-trait associations for spike-related characters in a doubled haploid population of wheat. in Romanian Agricultural Research. 2012;(29):9-16.
https://hdl.handle.net/21.15107/rcub_fiver_1154 .

Trkulja, Dragana, Kondić-Špika, Ankica, Brbaklić, Ljiljana, Kobiljski, Borislav, Hristov, Nikola, "Marker-trait associations for spike-related characters in a doubled haploid population of wheat" in Romanian Agricultural Research, no. 29 (2012):9-16,
https://hdl.handle.net/21.15107/rcub_fiver_1154 .

TY  - JOUR
AU  - Zorić, Miroslav
AU  - Dodig, Dejan
AU  - Kobiljski, Borislav
AU  - Quarrie, Steve
AU  - Barnes, Jeremy
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1090
AB  - A set of 96 winter wheat accessions sampled from a variety of geographic origins, including cultivars and breeding lines, were characterized with 46 genome-wide SSR loci for genetic diversity and population structure. The genetic diversity within these accessions was examined using a genetic distance-based and a model-based clustering method. The model-based analysis identified an underlying population structure comprising of four distinct sub-populations which corresponded well with distance-based groupings. Information on the population structure is taken into account in an association mapping study of grain yield from a 3-years field trial incorporating fully irrigated, rainfed and drought stress treatments. A total of 21 marker-grain yield associations (P  lt  0.01) were identified with nine SSR markers. Most associations were detected only in one to three environments (treatment/year combination), with an average R (2) value around 13 %. However, marker gwm484 (on chromosome 2D) was associated with yield in six environments, including irrigated, rainfed and drought stress treatments, suggesting it could be used to improve grain yield across a range of environments. Variation in grain yield at this locus was associated with earliness, early vigour, kernels per spikelet and harvest index. Microsatellite locus psp3200 (on chromosome 6D) was associated with yield in dry and hot environments, which was related to earliness, early vigour, productive tillering and total biomass per plant. Partial least squares regression, with nine environmental factors, showed that precipitation from tillering to maturity was the main environmental factor causing marker x environment associations for grain yield.
PB  - Springer, Dordrecht
T2  - Genetica
T1  - Population structure in a wheat core collection and genomic loci associated with yield under contrasting environments
EP  - 275
IS  - 4-6
SP  - 259
VL  - 140
DO  - 10.1007/s10709-012-9677-2
ER  - 

@article{
author = "Zorić, Miroslav and Dodig, Dejan and Kobiljski, Borislav and Quarrie, Steve and Barnes, Jeremy",
year = "2012",
abstract = "A set of 96 winter wheat accessions sampled from a variety of geographic origins, including cultivars and breeding lines, were characterized with 46 genome-wide SSR loci for genetic diversity and population structure. The genetic diversity within these accessions was examined using a genetic distance-based and a model-based clustering method. The model-based analysis identified an underlying population structure comprising of four distinct sub-populations which corresponded well with distance-based groupings. Information on the population structure is taken into account in an association mapping study of grain yield from a 3-years field trial incorporating fully irrigated, rainfed and drought stress treatments. A total of 21 marker-grain yield associations (P  lt  0.01) were identified with nine SSR markers. Most associations were detected only in one to three environments (treatment/year combination), with an average R (2) value around 13 %. However, marker gwm484 (on chromosome 2D) was associated with yield in six environments, including irrigated, rainfed and drought stress treatments, suggesting it could be used to improve grain yield across a range of environments. Variation in grain yield at this locus was associated with earliness, early vigour, kernels per spikelet and harvest index. Microsatellite locus psp3200 (on chromosome 6D) was associated with yield in dry and hot environments, which was related to earliness, early vigour, productive tillering and total biomass per plant. Partial least squares regression, with nine environmental factors, showed that precipitation from tillering to maturity was the main environmental factor causing marker x environment associations for grain yield.",
publisher = "Springer, Dordrecht",
journal = "Genetica",
title = "Population structure in a wheat core collection and genomic loci associated with yield under contrasting environments",
pages = "275-259",
number = "4-6",
volume = "140",
doi = "10.1007/s10709-012-9677-2"
}

Zorić, M., Dodig, D., Kobiljski, B., Quarrie, S.,& Barnes, J.. (2012). Population structure in a wheat core collection and genomic loci associated with yield under contrasting environments. in Genetica
Springer, Dordrecht., 140(4-6), 259-275.
https://doi.org/10.1007/s10709-012-9677-2

Zorić M, Dodig D, Kobiljski B, Quarrie S, Barnes J. Population structure in a wheat core collection and genomic loci associated with yield under contrasting environments. in Genetica. 2012;140(4-6):259-275.
doi:10.1007/s10709-012-9677-2 .

Zorić, Miroslav, Dodig, Dejan, Kobiljski, Borislav, Quarrie, Steve, Barnes, Jeremy, "Population structure in a wheat core collection and genomic loci associated with yield under contrasting environments" in Genetica, 140, no. 4-6 (2012):259-275,
https://doi.org/10.1007/s10709-012-9677-2 . .

TY  - JOUR
AU  - Kondić-Špika, Ankica
AU  - Vukosavljev, Mirjana
AU  - Kobiljski, Borislav
AU  - Hristov, Nikola
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/1040
AB  - The effect of two nutrient media, Potato-2 and N6, on androgenetic component traits and their relationships were studied in wheat anther culture. Six wheat (Triticum aestivum L.) F-1 combinations (NS 20 x Driada, MV 05-2001 x Lana, MV 05-2001 x Zitnica, Driada x NS 403/00, Driada x Dacia and Odeska 162 x NS KV 124/01A) were used as material for anther isolation. Variations, interrelationships and path analysis for five androgenetic component traits (androgenetic capacity: AC, embryoid induction: El, regeneration ability: RA, frequency of green plants: FOP and frequency of albino plants: FAP) were established. The average values for AC and El were significantly lower on N6 medium, while for RA, FOP and FAP there were no significant differences between the Potato-2 and N6 media. Correlation coefficient analyses showed positive correlation between AC and El (r = 0.99* on Potato-2 and r = 0.86* on N6), and between RA and FOP (r = 0.95* on Potato-2, r = 0.93* on N6), on both media. Additionally, the direct effects of RA and FAP on FOP were higly significant on both media, as determined by path coefficient, analysis. A trait-association using environment biplot was applied to understand the environmental effects on these relationships. The biplot showed that anther culture trait associations were differently responsive to the environment.
PB  - BMC
T2  - Journal of Biological Research
T1  - Relationships among androgenetic components in wheat and their responses to the environment
EP  - 223
SP  - 217
VL  - 16
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1040
ER  - 

@article{
author = "Kondić-Špika, Ankica and Vukosavljev, Mirjana and Kobiljski, Borislav and Hristov, Nikola",
year = "2011",
abstract = "The effect of two nutrient media, Potato-2 and N6, on androgenetic component traits and their relationships were studied in wheat anther culture. Six wheat (Triticum aestivum L.) F-1 combinations (NS 20 x Driada, MV 05-2001 x Lana, MV 05-2001 x Zitnica, Driada x NS 403/00, Driada x Dacia and Odeska 162 x NS KV 124/01A) were used as material for anther isolation. Variations, interrelationships and path analysis for five androgenetic component traits (androgenetic capacity: AC, embryoid induction: El, regeneration ability: RA, frequency of green plants: FOP and frequency of albino plants: FAP) were established. The average values for AC and El were significantly lower on N6 medium, while for RA, FOP and FAP there were no significant differences between the Potato-2 and N6 media. Correlation coefficient analyses showed positive correlation between AC and El (r = 0.99* on Potato-2 and r = 0.86* on N6), and between RA and FOP (r = 0.95* on Potato-2, r = 0.93* on N6), on both media. Additionally, the direct effects of RA and FAP on FOP were higly significant on both media, as determined by path coefficient, analysis. A trait-association using environment biplot was applied to understand the environmental effects on these relationships. The biplot showed that anther culture trait associations were differently responsive to the environment.",
publisher = "BMC",
journal = "Journal of Biological Research",
title = "Relationships among androgenetic components in wheat and their responses to the environment",
pages = "223-217",
volume = "16",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1040"
}

Kondić-Špika, A., Vukosavljev, M., Kobiljski, B.,& Hristov, N.. (2011). Relationships among androgenetic components in wheat and their responses to the environment. in Journal of Biological Research
BMC., 16, 217-223.
https://hdl.handle.net/21.15107/rcub_fiver_1040

Kondić-Špika A, Vukosavljev M, Kobiljski B, Hristov N. Relationships among androgenetic components in wheat and their responses to the environment. in Journal of Biological Research. 2011;16:217-223.
https://hdl.handle.net/21.15107/rcub_fiver_1040 .

Kondić-Špika, Ankica, Vukosavljev, Mirjana, Kobiljski, Borislav, Hristov, Nikola, "Relationships among androgenetic components in wheat and their responses to the environment" in Journal of Biological Research, 16 (2011):217-223,
https://hdl.handle.net/21.15107/rcub_fiver_1040 .

TY  - JOUR
AU  - Trkulja, Dragana
AU  - Kondić-Špika, Ankica
AU  - Brbaklić, Ljiljana
AU  - Kobiljski, Borislav
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/958
AB  - Doubled haploid (DH) mapping populations are suitable material for genetic analysis of quantitative trait loci (QTL). In order to detect loci associated with heading and flowering time in our agro-climate region, 177 lines of DH population Savana/Renesansa were analysed with five microsatellite markers (GWM18, GWM194, GWM261, PSP3071 and PSP3200). These lines were also evaluated for heading and flowering at the experimental field of the Institute of Field and Vegetable Crops in Novi Sad. The experiment was conducted from 2003 to 2008. According to Single Marker Regression analysis, the marker GWM261 was associated with QTL which explained from 20.2% to 30.7% of the total variance for heading and from 13.6% to 28.8% of the total variance for flowering time in all analysed years.
AB  - Dihaploidne mapirajuće populacije predstavljaju pogodan materijal za genetičku analizu lokusa koji utiču na ekspresiju kvantitativnih svojstava (QTL - Quantitative Trait Loci). U cilju detekcije lokusa koji su u vezi sa vremenom klasanja i cvetanja u našem agro-klimatskom regionu, u radu je analizirana alelna varijabilnost pet mikrosatelitskih lokusa (Xgwm18 Xgwm194, Xgwm261, Xpsp3071 i Xpsp3200) kod 177 linija dihaploidne populacije Savana/Renesansa. Pored toga, na oglednim poljima Instituta za ratarstvo i povrtarstvo u Novom Sadu u periodu 2003-2008. rađena je fenotipska evaluacija ove mapirajuće populacije za navedene osobine. Metodom pojedinačne marker regresije detektovan je QTL u blizini lokusa Xgwm261, pomoću kog se moglo objasniti od 20,2% do 30,7% fenotipske varijabilnosti za vreme klasanja i 13,6% do 28,8% za vreme cvetanja, u svim analiziranim godinama.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Marker-trait association analysis for heading and flowering time in wheat by Single Marker Regression
T1  - Analiza veze marker-svojstvo za vreme klasanja i cvetanja pšenice korišćenjem pojedinačne marker regresije
EP  - 120
IS  - 1
SP  - 113
VL  - 48
DO  - 10.5937/ratpov1101113T
ER  - 

@article{
author = "Trkulja, Dragana and Kondić-Špika, Ankica and Brbaklić, Ljiljana and Kobiljski, Borislav",
year = "2011",
abstract = "Doubled haploid (DH) mapping populations are suitable material for genetic analysis of quantitative trait loci (QTL). In order to detect loci associated with heading and flowering time in our agro-climate region, 177 lines of DH population Savana/Renesansa were analysed with five microsatellite markers (GWM18, GWM194, GWM261, PSP3071 and PSP3200). These lines were also evaluated for heading and flowering at the experimental field of the Institute of Field and Vegetable Crops in Novi Sad. The experiment was conducted from 2003 to 2008. According to Single Marker Regression analysis, the marker GWM261 was associated with QTL which explained from 20.2% to 30.7% of the total variance for heading and from 13.6% to 28.8% of the total variance for flowering time in all analysed years., Dihaploidne mapirajuće populacije predstavljaju pogodan materijal za genetičku analizu lokusa koji utiču na ekspresiju kvantitativnih svojstava (QTL - Quantitative Trait Loci). U cilju detekcije lokusa koji su u vezi sa vremenom klasanja i cvetanja u našem agro-klimatskom regionu, u radu je analizirana alelna varijabilnost pet mikrosatelitskih lokusa (Xgwm18 Xgwm194, Xgwm261, Xpsp3071 i Xpsp3200) kod 177 linija dihaploidne populacije Savana/Renesansa. Pored toga, na oglednim poljima Instituta za ratarstvo i povrtarstvo u Novom Sadu u periodu 2003-2008. rađena je fenotipska evaluacija ove mapirajuće populacije za navedene osobine. Metodom pojedinačne marker regresije detektovan je QTL u blizini lokusa Xgwm261, pomoću kog se moglo objasniti od 20,2% do 30,7% fenotipske varijabilnosti za vreme klasanja i 13,6% do 28,8% za vreme cvetanja, u svim analiziranim godinama.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Marker-trait association analysis for heading and flowering time in wheat by Single Marker Regression, Analiza veze marker-svojstvo za vreme klasanja i cvetanja pšenice korišćenjem pojedinačne marker regresije",
pages = "120-113",
number = "1",
volume = "48",
doi = "10.5937/ratpov1101113T"
}

Trkulja, D., Kondić-Špika, A., Brbaklić, L.,& Kobiljski, B.. (2011). Marker-trait association analysis for heading and flowering time in wheat by Single Marker Regression. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(1), 113-120.
https://doi.org/10.5937/ratpov1101113T

Trkulja D, Kondić-Špika A, Brbaklić L, Kobiljski B. Marker-trait association analysis for heading and flowering time in wheat by Single Marker Regression. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2011;48(1):113-120.
doi:10.5937/ratpov1101113T .

Trkulja, Dragana, Kondić-Špika, Ankica, Brbaklić, Ljiljana, Kobiljski, Borislav, "Marker-trait association analysis for heading and flowering time in wheat by Single Marker Regression" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 48, no. 1 (2011):113-120,
https://doi.org/10.5937/ratpov1101113T . .

TY  - JOUR
AU  - Treskić, Sanja
AU  - Ivanović, Mile
AU  - Kobiljski, Borislav
AU  - Kondić-Špika, Ankica
AU  - Brbaklić, Ljiljana
AU  - Trkulja, Dragana
AU  - Stanisavljević, Dušan
AU  - Mitrović, Bojan
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/953
AB  - Today the marker assisted selection (MAS) is being routinely applied in breeding programs of large private companies. However, the implementation of molecular markers for commercial use in small companies and public sec- tor is on a considerably smaller scale. Numerous researches on QTL mapping, theoretical analysis and simulation models for MAS give impetus to new research on the validation of quantitative trait loci and the application of molecular markers in maize breeding. This paper presents basic concepts related to MAS, the principles of QTL mapping, marker-trait association analysis and examples of successful application of markers in breeding for qualitative and quantitative traits.
AB  - Iako se danas u svetu marker asistirana selekcija (MAS) rutinski primenjuje u oplemenjivačkim programima velikih privatnih kompanija, rad na implementaciji molekularnih markera za komercijalne potrebe u manjim kompanijama i u javnom sektoru je značajno manjeg obima. Brojni radovi o mapiranju lokusa za kvantitativna svojstva, teorijske analize i simulacioni modeli o mogućnostima MAS, daju podsticaj novim istraživanjima na polju validacije otkrivenih QTL-ova (Quantitative Trait Loci), kao i praktične primene molekularnih markera u oplemenjivanju kukuruza. U radu su objašnjeni osnovni pojmovi koji se odnose na MAS, principe QTL mapiranja i utvrđivanja veze marker-svojstvo, predstavljene su mogućnosti i primeri uspešne primene markera u selekciji na kvalitativne i kvantitativne osobine.
PB  - Društvo selekcionara i semenara Republike Srbije, Beograd
T2  - Selekcija i semenarstvo
T1  - Molecular markers in maize breeding
T1  - Molekularni markeri u oplemenjivanju kukuruza
EP  - 81
IS  - 1
SP  - 71
VL  - 17
UR  - https://hdl.handle.net/21.15107/rcub_fiver_953
ER  - 

@article{
author = "Treskić, Sanja and Ivanović, Mile and Kobiljski, Borislav and Kondić-Špika, Ankica and Brbaklić, Ljiljana and Trkulja, Dragana and Stanisavljević, Dušan and Mitrović, Bojan",
year = "2011",
abstract = "Today the marker assisted selection (MAS) is being routinely applied in breeding programs of large private companies. However, the implementation of molecular markers for commercial use in small companies and public sec- tor is on a considerably smaller scale. Numerous researches on QTL mapping, theoretical analysis and simulation models for MAS give impetus to new research on the validation of quantitative trait loci and the application of molecular markers in maize breeding. This paper presents basic concepts related to MAS, the principles of QTL mapping, marker-trait association analysis and examples of successful application of markers in breeding for qualitative and quantitative traits., Iako se danas u svetu marker asistirana selekcija (MAS) rutinski primenjuje u oplemenjivačkim programima velikih privatnih kompanija, rad na implementaciji molekularnih markera za komercijalne potrebe u manjim kompanijama i u javnom sektoru je značajno manjeg obima. Brojni radovi o mapiranju lokusa za kvantitativna svojstva, teorijske analize i simulacioni modeli o mogućnostima MAS, daju podsticaj novim istraživanjima na polju validacije otkrivenih QTL-ova (Quantitative Trait Loci), kao i praktične primene molekularnih markera u oplemenjivanju kukuruza. U radu su objašnjeni osnovni pojmovi koji se odnose na MAS, principe QTL mapiranja i utvrđivanja veze marker-svojstvo, predstavljene su mogućnosti i primeri uspešne primene markera u selekciji na kvalitativne i kvantitativne osobine.",
publisher = "Društvo selekcionara i semenara Republike Srbije, Beograd",
journal = "Selekcija i semenarstvo",
title = "Molecular markers in maize breeding, Molekularni markeri u oplemenjivanju kukuruza",
pages = "81-71",
number = "1",
volume = "17",
url = "https://hdl.handle.net/21.15107/rcub_fiver_953"
}

Treskić, S., Ivanović, M., Kobiljski, B., Kondić-Špika, A., Brbaklić, L., Trkulja, D., Stanisavljević, D.,& Mitrović, B.. (2011). Molecular markers in maize breeding. in Selekcija i semenarstvo
Društvo selekcionara i semenara Republike Srbije, Beograd., 17(1), 71-81.
https://hdl.handle.net/21.15107/rcub_fiver_953

Treskić S, Ivanović M, Kobiljski B, Kondić-Špika A, Brbaklić L, Trkulja D, Stanisavljević D, Mitrović B. Molecular markers in maize breeding. in Selekcija i semenarstvo. 2011;17(1):71-81.
https://hdl.handle.net/21.15107/rcub_fiver_953 .

Treskić, Sanja, Ivanović, Mile, Kobiljski, Borislav, Kondić-Špika, Ankica, Brbaklić, Ljiljana, Trkulja, Dragana, Stanisavljević, Dušan, Mitrović, Bojan, "Molecular markers in maize breeding" in Selekcija i semenarstvo, 17, no. 1 (2011):71-81,
https://hdl.handle.net/21.15107/rcub_fiver_953 .

TY  - JOUR
AU  - Dodig, Dejan
AU  - Zorić, Miroslav
AU  - Kobiljski, Borislav
AU  - Šurlan-Momirović, Gordana
AU  - Quarrie, Steve
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/841
AB  - This study was conducted to assess drought tolerance and regional-based patterns of diversity of bread wheat accessions and to identify new sources of diversity that could accelerate the development of improved wheat varieties better suited to meeting the challenges posed by changing climate in Southern and Eastern Europe. For this, genetic diversity assessed by simple sequence repeats (SSR) markers was compared with diversity evaluated using 19 phenotypic traits averaged over irrigated and drought-stress field conditions. Thirty-six SSR were used to profile 96 wheat genotypes from the collection of genetic resources at the Institute of Field and Vegetable Crops, Novi Sad, Serbia. A total of 46 loci and 366 alleles were detected, with a range of 3-21 alleles per locus. The polymorphic information content was estimated to be 0.61. The genetic distance for all possible 4560 pairs of genotypes ranged from 0.06 to 0.91 with an average of 0.65. Genotypes were grouped according to their drought tolerance (high, medium, low) and region of origin. Analysis of molecular variance showed that over 96% of the total variation could be explained by the variance within the drought tolerance and geographical groups. As a whole, genetic diversity among the high drought tolerance genotypes was considerably higher than that among low drought tolerance genotypes. Comparative analysis of SSR diversity among six regional groups revealed that the genotypes from North America exhibited more genetic diversity than those from other regions. Two dendrograms were constructed based on phenotypic and molecular analyses using the Unweighted Pair Group Method with Arithmetic Mean method and were found to be topologically different. Genotypes characterised as highly drought tolerant were distributed among all SSR-based cluster groups. This implied that the genetic basis of drought tolerance in these genotypes was different, thereby enabling wheat breeders to combine these diverse sources of genetic variability to improve drought tolerance in their breeding programs.
PB  - CSIRO Publishing, Collingwood
T2  - Crop & Pasture Science
T1  - Assessing drought tolerance and regional patterns of genetic diversity among spring and winter bread wheat using simple sequence repeats and phenotypic data
EP  - 824
IS  - 10
SP  - 812
VL  - 61
DO  - 10.1071/CP10001
ER  - 

@article{
author = "Dodig, Dejan and Zorić, Miroslav and Kobiljski, Borislav and Šurlan-Momirović, Gordana and Quarrie, Steve",
year = "2010",
abstract = "This study was conducted to assess drought tolerance and regional-based patterns of diversity of bread wheat accessions and to identify new sources of diversity that could accelerate the development of improved wheat varieties better suited to meeting the challenges posed by changing climate in Southern and Eastern Europe. For this, genetic diversity assessed by simple sequence repeats (SSR) markers was compared with diversity evaluated using 19 phenotypic traits averaged over irrigated and drought-stress field conditions. Thirty-six SSR were used to profile 96 wheat genotypes from the collection of genetic resources at the Institute of Field and Vegetable Crops, Novi Sad, Serbia. A total of 46 loci and 366 alleles were detected, with a range of 3-21 alleles per locus. The polymorphic information content was estimated to be 0.61. The genetic distance for all possible 4560 pairs of genotypes ranged from 0.06 to 0.91 with an average of 0.65. Genotypes were grouped according to their drought tolerance (high, medium, low) and region of origin. Analysis of molecular variance showed that over 96% of the total variation could be explained by the variance within the drought tolerance and geographical groups. As a whole, genetic diversity among the high drought tolerance genotypes was considerably higher than that among low drought tolerance genotypes. Comparative analysis of SSR diversity among six regional groups revealed that the genotypes from North America exhibited more genetic diversity than those from other regions. Two dendrograms were constructed based on phenotypic and molecular analyses using the Unweighted Pair Group Method with Arithmetic Mean method and were found to be topologically different. Genotypes characterised as highly drought tolerant were distributed among all SSR-based cluster groups. This implied that the genetic basis of drought tolerance in these genotypes was different, thereby enabling wheat breeders to combine these diverse sources of genetic variability to improve drought tolerance in their breeding programs.",
publisher = "CSIRO Publishing, Collingwood",
journal = "Crop & Pasture Science",
title = "Assessing drought tolerance and regional patterns of genetic diversity among spring and winter bread wheat using simple sequence repeats and phenotypic data",
pages = "824-812",
number = "10",
volume = "61",
doi = "10.1071/CP10001"
}

Dodig, D., Zorić, M., Kobiljski, B., Šurlan-Momirović, G.,& Quarrie, S.. (2010). Assessing drought tolerance and regional patterns of genetic diversity among spring and winter bread wheat using simple sequence repeats and phenotypic data. in Crop & Pasture Science
CSIRO Publishing, Collingwood., 61(10), 812-824.
https://doi.org/10.1071/CP10001

Dodig D, Zorić M, Kobiljski B, Šurlan-Momirović G, Quarrie S. Assessing drought tolerance and regional patterns of genetic diversity among spring and winter bread wheat using simple sequence repeats and phenotypic data. in Crop & Pasture Science. 2010;61(10):812-824.
doi:10.1071/CP10001 .

Dodig, Dejan, Zorić, Miroslav, Kobiljski, Borislav, Šurlan-Momirović, Gordana, Quarrie, Steve, "Assessing drought tolerance and regional patterns of genetic diversity among spring and winter bread wheat using simple sequence repeats and phenotypic data" in Crop & Pasture Science, 61, no. 10 (2010):812-824,
https://doi.org/10.1071/CP10001 . .

TY  - JOUR
AU  - Brdar-Jokanović, Milka
AU  - Maksimović, Ivana
AU  - Nikolić-Đorić, Emilija
AU  - Kraljević-Balalić, Marija
AU  - Kobiljski, Borislav
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/913
AB  - Excess boron may exhibit detrimental effect on wheat (Triticum aestivum L.). An effective technique for assessing the response of wheat genotypes to excess boron is required to create high yielding tolerant cultivars. Filter paper assay, based on seedling's primary root length reduction in the presence of excess boron, is commonly used for distinguishing tolerant and sensitive genotypes. The study was undertaken to investigate the effect of excess boron on both primary and lateral root length, number of roots, number of days from imbibition to germination and germination percentage on the sample of 25 wheat cultivars, differing in origin and boron tolerance. The experiment included control and three boron treatments. On average, excess boron reduced root length and number and had no effect on number of days from imbibition to germination and germination percentage; however, significant differences have been found among the genotypes. The imposed boron treatments demonstrated 5.2% stronger effect on lateral root length in comparison to primary root length. In 10 out of 25 cases, boron tolerance estimated from primary root length reduction was not consistent with the estimation from lateral root length reduction; therefore, total root length reduction may be more valuable selection criterion for boron tolerance in wheat.
T2  - Pakistan Journal of Botany
T1  - Selection criterion to assess wheat boron tolerance at seedling stage: primary vs. Total root length
EP  - 3947
IS  - 6
SP  - 3939
VL  - 42
UR  - https://hdl.handle.net/21.15107/rcub_fiver_913
ER  - 

@article{
author = "Brdar-Jokanović, Milka and Maksimović, Ivana and Nikolić-Đorić, Emilija and Kraljević-Balalić, Marija and Kobiljski, Borislav",
year = "2010",
abstract = "Excess boron may exhibit detrimental effect on wheat (Triticum aestivum L.). An effective technique for assessing the response of wheat genotypes to excess boron is required to create high yielding tolerant cultivars. Filter paper assay, based on seedling's primary root length reduction in the presence of excess boron, is commonly used for distinguishing tolerant and sensitive genotypes. The study was undertaken to investigate the effect of excess boron on both primary and lateral root length, number of roots, number of days from imbibition to germination and germination percentage on the sample of 25 wheat cultivars, differing in origin and boron tolerance. The experiment included control and three boron treatments. On average, excess boron reduced root length and number and had no effect on number of days from imbibition to germination and germination percentage; however, significant differences have been found among the genotypes. The imposed boron treatments demonstrated 5.2% stronger effect on lateral root length in comparison to primary root length. In 10 out of 25 cases, boron tolerance estimated from primary root length reduction was not consistent with the estimation from lateral root length reduction; therefore, total root length reduction may be more valuable selection criterion for boron tolerance in wheat.",
journal = "Pakistan Journal of Botany",
title = "Selection criterion to assess wheat boron tolerance at seedling stage: primary vs. Total root length",
pages = "3947-3939",
number = "6",
volume = "42",
url = "https://hdl.handle.net/21.15107/rcub_fiver_913"
}

Brdar-Jokanović, M., Maksimović, I., Nikolić-Đorić, E., Kraljević-Balalić, M.,& Kobiljski, B.. (2010). Selection criterion to assess wheat boron tolerance at seedling stage: primary vs. Total root length. in Pakistan Journal of Botany, 42(6), 3939-3947.
https://hdl.handle.net/21.15107/rcub_fiver_913

Brdar-Jokanović M, Maksimović I, Nikolić-Đorić E, Kraljević-Balalić M, Kobiljski B. Selection criterion to assess wheat boron tolerance at seedling stage: primary vs. Total root length. in Pakistan Journal of Botany. 2010;42(6):3939-3947.
https://hdl.handle.net/21.15107/rcub_fiver_913 .

Brdar-Jokanović, Milka, Maksimović, Ivana, Nikolić-Đorić, Emilija, Kraljević-Balalić, Marija, Kobiljski, Borislav, "Selection criterion to assess wheat boron tolerance at seedling stage: primary vs. Total root length" in Pakistan Journal of Botany, 42, no. 6 (2010):3939-3947,
https://hdl.handle.net/21.15107/rcub_fiver_913 .

TY  - JOUR
AU  - Denčić, Srbislav
AU  - Kobiljski, Borislav
AU  - Mladenović, Gojko
AU  - Jestrović, Zorica
AU  - Štatkić, Slaviša
AU  - Pavlović, Milenko
AU  - Orbović, Branka
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/889
AB  - Analiziran je prosečan prinos pšenice u Srbiji i nekoliko stranih zemalja u 20. veku. Ispoljile su se tri zajedničke karakteristike: a) u prvih 50 godina prošlog veka nije bilo značajnog povećanja prosečnih prinosa; b) u drugih 50 godina došlo je do dramatičnog povećanja prosečnih prinosa za čak 230 % do 280 %; c) u poslednjih 15-tak godina nije zabeležen značajniji porast prosečnih prinosa. Razlozi za rapidno povećanje prosečnih prinosa u drugoj polovini 20. veka su uvođenje polupatuljastih sorti u proizvodnju i upotreba većih količina mineralnih đubriva. Savremene sorte polupatuljaste stabljike sa većim potencijalom za prinos u odnosu na sorte sa visokom stabljikom pokazuju i veće variranje između maksimalnih i minimalnih prinosa, kao i između godina. Te sorte pokazuju i veoma pozitivnu reakciju na dobru agrotehniku. Sorta kao faktor daljeg povećanja prinosa, a time i proizvodnje, nije izgubila u potpunosti svoj značaj, samo što su dalji napreci mali i često statistički nesignifikantni. U protekloj sezoni u Republici Srbiji prinosi i proizvodnja pšenice su bili na nivou poslednjeg desetogodišnjeg proseka. Dominantne sorte u proizvodnji su bile Pobeda, Renesansa i Evropa 90 a zauzimale su preko 60 % ukupnih površina pod pšenicom. U mreži makro-ogleda u Srbiji najbolje rezultate su postigle sorte Arija, Zvezdana, Etida i NS 40S. .
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Variety as a factor in wheat production
T1  - Sorta kao faktor proizvodnje pšenice
EP  - 324
IS  - 1
SP  - 317
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_fiver_889
ER  - 

@article{
author = "Denčić, Srbislav and Kobiljski, Borislav and Mladenović, Gojko and Jestrović, Zorica and Štatkić, Slaviša and Pavlović, Milenko and Orbović, Branka",
year = "2010",
abstract = "Analiziran je prosečan prinos pšenice u Srbiji i nekoliko stranih zemalja u 20. veku. Ispoljile su se tri zajedničke karakteristike: a) u prvih 50 godina prošlog veka nije bilo značajnog povećanja prosečnih prinosa; b) u drugih 50 godina došlo je do dramatičnog povećanja prosečnih prinosa za čak 230 % do 280 %; c) u poslednjih 15-tak godina nije zabeležen značajniji porast prosečnih prinosa. Razlozi za rapidno povećanje prosečnih prinosa u drugoj polovini 20. veka su uvođenje polupatuljastih sorti u proizvodnju i upotreba većih količina mineralnih đubriva. Savremene sorte polupatuljaste stabljike sa većim potencijalom za prinos u odnosu na sorte sa visokom stabljikom pokazuju i veće variranje između maksimalnih i minimalnih prinosa, kao i između godina. Te sorte pokazuju i veoma pozitivnu reakciju na dobru agrotehniku. Sorta kao faktor daljeg povećanja prinosa, a time i proizvodnje, nije izgubila u potpunosti svoj značaj, samo što su dalji napreci mali i često statistički nesignifikantni. U protekloj sezoni u Republici Srbiji prinosi i proizvodnja pšenice su bili na nivou poslednjeg desetogodišnjeg proseka. Dominantne sorte u proizvodnji su bile Pobeda, Renesansa i Evropa 90 a zauzimale su preko 60 % ukupnih površina pod pšenicom. U mreži makro-ogleda u Srbiji najbolje rezultate su postigle sorte Arija, Zvezdana, Etida i NS 40S. .",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Variety as a factor in wheat production, Sorta kao faktor proizvodnje pšenice",
pages = "324-317",
number = "1",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_fiver_889"
}

Denčić, S., Kobiljski, B., Mladenović, G., Jestrović, Z., Štatkić, S., Pavlović, M.,& Orbović, B.. (2010). Variety as a factor in wheat production. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 47(1), 317-324.
https://hdl.handle.net/21.15107/rcub_fiver_889

Denčić S, Kobiljski B, Mladenović G, Jestrović Z, Štatkić S, Pavlović M, Orbović B. Variety as a factor in wheat production. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2010;47(1):317-324.
https://hdl.handle.net/21.15107/rcub_fiver_889 .

Denčić, Srbislav, Kobiljski, Borislav, Mladenović, Gojko, Jestrović, Zorica, Štatkić, Slaviša, Pavlović, Milenko, Orbović, Branka, "Variety as a factor in wheat production" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 47, no. 1 (2010):317-324,
https://hdl.handle.net/21.15107/rcub_fiver_889 .

TY  - JOUR
AU  - Kondić-Špika, Ankica
AU  - Kobiljski, Borislav
AU  - Marjanović, Milica
AU  - Hristov, Nikola
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/874
AB  - Wheat tolerance to high boron concentrations was determined using mature embryo culture. The testing was performed on a modified MS nutrient medium to which boric acid was added in two concentrations: 15 mM and 30 mM. The control medium contained no excess boric acid. The experiment involved 14 Serbian varieties. After one month of cultivation callus fresh weight (CFW) was measured and reductions of fresh callus weight (RFCW) at boron (B) concentration of 15 mM in relation to the control were calculated. The genotypes differed significantly according to their reaction to different B concentrations. Six genotypes had RFCWs below 50.0 %, while eight genotypes had RFCWs above 50.1 %. Cultivars Nevesinjka and Pesma were considered the most tolerant genotypes, while cultivars Balada and Vila were the most sensitive. The results confirmed that differences in genotype reactions to excess B are visible at the cellular level and that they may serve as a selection criterion.
AB  - Tolerantnost pšenice prema visokim koncentracijama bora ispitana je primenom kulture zrelog embriona. Testiranje je vršeno na modifikovanoj MS hranljivoj podlozi, kojoj je borna kiselina dodata u dve koncentracije: 15 mM i 30 mM. Kontrolna podloga nije sadržala bornu kiselinu u suvišku. Eksperiment je izveden na 14 sorti pšenice proizvedenih u Srbiji. Posle mesec dana gajenja na podlogama izmerena je sveža masa kalusa (SMK) i izračunata redukcija sveže mase kalusa (RSMK) na podlozi sa 15 mM borne kiseline, u odnosu na kontrolu. Genotipovi su se značajno razlikovali u pogledu njihove reakcije na različite koncentracije bora. Šest genotipova imalo je RSMK ispod 50,0 %, dok je osam genotipova imalo RSMK iznad 50,1 %. Sorte Nevesinjka i Pesma ocenjene su kao najtolerantnije, dok su sorte Balada i Vila bile najosetljivije na prisustvo bora u podlozi. Rezultati su potvrdili da su reakcije genotipova pšenice na suvišak bora vidljive i na ćelijskom nivou, te da se ova reakcija može koristiti kao selekcioni kriterijum za procenu tolerantnosti na bor.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes
T1  - In vitro evaluacija tolerantnosti genotipova pšenice (Triticum aestivum L.) prema boru
EP  - 91
IS  - 1
SP  - 85
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_fiver_874
ER  - 

@article{
author = "Kondić-Špika, Ankica and Kobiljski, Borislav and Marjanović, Milica and Hristov, Nikola",
year = "2010",
abstract = "Wheat tolerance to high boron concentrations was determined using mature embryo culture. The testing was performed on a modified MS nutrient medium to which boric acid was added in two concentrations: 15 mM and 30 mM. The control medium contained no excess boric acid. The experiment involved 14 Serbian varieties. After one month of cultivation callus fresh weight (CFW) was measured and reductions of fresh callus weight (RFCW) at boron (B) concentration of 15 mM in relation to the control were calculated. The genotypes differed significantly according to their reaction to different B concentrations. Six genotypes had RFCWs below 50.0 %, while eight genotypes had RFCWs above 50.1 %. Cultivars Nevesinjka and Pesma were considered the most tolerant genotypes, while cultivars Balada and Vila were the most sensitive. The results confirmed that differences in genotype reactions to excess B are visible at the cellular level and that they may serve as a selection criterion., Tolerantnost pšenice prema visokim koncentracijama bora ispitana je primenom kulture zrelog embriona. Testiranje je vršeno na modifikovanoj MS hranljivoj podlozi, kojoj je borna kiselina dodata u dve koncentracije: 15 mM i 30 mM. Kontrolna podloga nije sadržala bornu kiselinu u suvišku. Eksperiment je izveden na 14 sorti pšenice proizvedenih u Srbiji. Posle mesec dana gajenja na podlogama izmerena je sveža masa kalusa (SMK) i izračunata redukcija sveže mase kalusa (RSMK) na podlozi sa 15 mM borne kiseline, u odnosu na kontrolu. Genotipovi su se značajno razlikovali u pogledu njihove reakcije na različite koncentracije bora. Šest genotipova imalo je RSMK ispod 50,0 %, dok je osam genotipova imalo RSMK iznad 50,1 %. Sorte Nevesinjka i Pesma ocenjene su kao najtolerantnije, dok su sorte Balada i Vila bile najosetljivije na prisustvo bora u podlozi. Rezultati su potvrdili da su reakcije genotipova pšenice na suvišak bora vidljive i na ćelijskom nivou, te da se ova reakcija može koristiti kao selekcioni kriterijum za procenu tolerantnosti na bor.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes, In vitro evaluacija tolerantnosti genotipova pšenice (Triticum aestivum L.) prema boru",
pages = "91-85",
number = "1",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_fiver_874"
}

Kondić-Špika, A., Kobiljski, B., Marjanović, M.,& Hristov, N.. (2010). In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 47(1), 85-91.
https://hdl.handle.net/21.15107/rcub_fiver_874

Kondić-Špika A, Kobiljski B, Marjanović M, Hristov N. In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2010;47(1):85-91.
https://hdl.handle.net/21.15107/rcub_fiver_874 .

Kondić-Špika, Ankica, Kobiljski, Borislav, Marjanović, Milica, Hristov, Nikola, "In vitro evaluation of boron tolerance in wheat (Triticum aestivum L.) genotypes" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 47, no. 1 (2010):85-91,
https://hdl.handle.net/21.15107/rcub_fiver_874 .