TY  - JOUR
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Prokić, Anđelka
AU  - Kuzmanović, Nemanja
AU  - Ignjatov, Maja
AU  - Obradović, Aleksa
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1141
AB  - Bacteriophages are viruses that infect bacteria. Due to poor efficacy of copper compounds and occurrence of antibiotic resistant bacterial strains, interest in phage therapy has increased in the recent years. In addition to being used for disease control, the high specificity of bacteriophages makes them useful in differentiation of closely related species of plant pathogenic bacteria. In this research, twenty five phages, specific to Xanthomonas euvesicatoria, causal agent of pepper bacterial spot, were isolated. Host specificity determination performed with 53 phages from University of Florida collection resulted in set of phages which could be used for differentiation of X. euvesicatoria, X. perforans, X. vesicatoria and X. gardneri.
AB  - Bakteriofagi predstavljaju posebnu grupu virusa čiji su domaćini bakterije. Usled nedostatka efikasnih baktericida i pojave rezistentnih sojeva bakterija prema većini do sada korišćenih antibiotika, primena faga kao bioloških agenasa u kontroli bolesti prouzrokovanih fitopatogenim bakterijama, dobija sve veći značaj. Zahvaljujući izraženoj specifičnosti, fagi se osim za suzbijanje koriste i za diferencijaciju srodnih vrsta fitopatogenih bakterija. U ovom radu izolovano je 25 faga specifičnih prema vrsti Xanthomonas euvesicatoria, prouzrokovaču bakteriozne pegavosti paprike. Fagi su izolovani iz uzoraka zemljišta, vode i semena paprike poreklom iz različitih lokaliteta u Srbiji. Pored faga izolovanih u Srbiji, proverena je specifičnost 53 soja dobijenih iz kolekcije Univerziteta u Floridi i izdvojen set faga koji se mogu koristiti u diferencijaciji vrsta X. euvesicatoria, X. perforans, X. vesicatoria i X. gardneri.
PB  - Institut za zaštitu bilja i životnu sredinu, Beograd
T2  - Zaštita bilja
T1  - Isolation of bacteriophages and their use in Xanthomonas spp. differantiation
T1  - Izolacija bakteriofaga i njihova primena u diferencijaciji sojeva Xanthomonas spp.
EP  - 75
IS  - 2
SP  - 62
VL  - 63
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1141
ER  - 

@article{
author = "Gašić, Katarina and Ivanović, Milan and Prokić, Anđelka and Kuzmanović, Nemanja and Ignjatov, Maja and Obradović, Aleksa",
year = "2012",
abstract = "Bacteriophages are viruses that infect bacteria. Due to poor efficacy of copper compounds and occurrence of antibiotic resistant bacterial strains, interest in phage therapy has increased in the recent years. In addition to being used for disease control, the high specificity of bacteriophages makes them useful in differentiation of closely related species of plant pathogenic bacteria. In this research, twenty five phages, specific to Xanthomonas euvesicatoria, causal agent of pepper bacterial spot, were isolated. Host specificity determination performed with 53 phages from University of Florida collection resulted in set of phages which could be used for differentiation of X. euvesicatoria, X. perforans, X. vesicatoria and X. gardneri., Bakteriofagi predstavljaju posebnu grupu virusa čiji su domaćini bakterije. Usled nedostatka efikasnih baktericida i pojave rezistentnih sojeva bakterija prema većini do sada korišćenih antibiotika, primena faga kao bioloških agenasa u kontroli bolesti prouzrokovanih fitopatogenim bakterijama, dobija sve veći značaj. Zahvaljujući izraženoj specifičnosti, fagi se osim za suzbijanje koriste i za diferencijaciju srodnih vrsta fitopatogenih bakterija. U ovom radu izolovano je 25 faga specifičnih prema vrsti Xanthomonas euvesicatoria, prouzrokovaču bakteriozne pegavosti paprike. Fagi su izolovani iz uzoraka zemljišta, vode i semena paprike poreklom iz različitih lokaliteta u Srbiji. Pored faga izolovanih u Srbiji, proverena je specifičnost 53 soja dobijenih iz kolekcije Univerziteta u Floridi i izdvojen set faga koji se mogu koristiti u diferencijaciji vrsta X. euvesicatoria, X. perforans, X. vesicatoria i X. gardneri.",
publisher = "Institut za zaštitu bilja i životnu sredinu, Beograd",
journal = "Zaštita bilja",
title = "Isolation of bacteriophages and their use in Xanthomonas spp. differantiation, Izolacija bakteriofaga i njihova primena u diferencijaciji sojeva Xanthomonas spp.",
pages = "75-62",
number = "2",
volume = "63",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1141"
}

Gašić, K., Ivanović, M., Prokić, A., Kuzmanović, N., Ignjatov, M.,& Obradović, A.. (2012). Isolation of bacteriophages and their use in Xanthomonas spp. differantiation. in Zaštita bilja
Institut za zaštitu bilja i životnu sredinu, Beograd., 63(2), 62-75.
https://hdl.handle.net/21.15107/rcub_fiver_1141

Gašić K, Ivanović M, Prokić A, Kuzmanović N, Ignjatov M, Obradović A. Isolation of bacteriophages and their use in Xanthomonas spp. differantiation. in Zaštita bilja. 2012;63(2):62-75.
https://hdl.handle.net/21.15107/rcub_fiver_1141 .

Gašić, Katarina, Ivanović, Milan, Prokić, Anđelka, Kuzmanović, Nemanja, Ignjatov, Maja, Obradović, Aleksa, "Isolation of bacteriophages and their use in Xanthomonas spp. differantiation" in Zaštita bilja, 63, no. 2 (2012):62-75,
https://hdl.handle.net/21.15107/rcub_fiver_1141 .

TY  - JOUR
AU  - Gagić, K.
AU  - Ivanović, Milan
AU  - Ignjatov, Maja
AU  - Ćalić, Irena
AU  - Obradović, Aleksa
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/1051
AB  - Host range, plaque morphology, thermal inactivation point, genome size and restriction fragment patterns of ten bacteriophage isolates originating from soil, pepper seed and irrigation water collected from five localities in Serbia were studied. The bacteriophage isolates were selected based on their specificity to Xanthomonas euvesicatoria, causal agent of bacterial spot of pepper. The phages had similar plaque morphology, except for two isolates producing a plaque-surrounding halo in culture of X. euvesicatoria strain KFB 189. Four phage isolates were inactivated at 70 degrees C and six at 71 degrees C. All phages had genome size of approximately 22 kb and were differentiated into four types by their EcoRI and BamHI restriction fragment patterns. Examination of two phages by transmission electron microscopy classified them as A1 morphotype members of the Myoviridae family, order Caudovirales. Although specific to X. euvesicatoria, the phages were differentiated into three groups based on their ability to lyse 59 strains of this bacterium. Adsorption rates and one-step growth curves were determined for each group representative phage isolates.
T2  - Journal of Plant Pathology
T1  - Isolation and characterization of xanthomonas euvesicatoria bacteriophages
EP  - 423
IS  - 2
SP  - 415
VL  - 93
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1051
ER  - 

@article{
author = "Gagić, K. and Ivanović, Milan and Ignjatov, Maja and Ćalić, Irena and Obradović, Aleksa",
year = "2011",
abstract = "Host range, plaque morphology, thermal inactivation point, genome size and restriction fragment patterns of ten bacteriophage isolates originating from soil, pepper seed and irrigation water collected from five localities in Serbia were studied. The bacteriophage isolates were selected based on their specificity to Xanthomonas euvesicatoria, causal agent of bacterial spot of pepper. The phages had similar plaque morphology, except for two isolates producing a plaque-surrounding halo in culture of X. euvesicatoria strain KFB 189. Four phage isolates were inactivated at 70 degrees C and six at 71 degrees C. All phages had genome size of approximately 22 kb and were differentiated into four types by their EcoRI and BamHI restriction fragment patterns. Examination of two phages by transmission electron microscopy classified them as A1 morphotype members of the Myoviridae family, order Caudovirales. Although specific to X. euvesicatoria, the phages were differentiated into three groups based on their ability to lyse 59 strains of this bacterium. Adsorption rates and one-step growth curves were determined for each group representative phage isolates.",
journal = "Journal of Plant Pathology",
title = "Isolation and characterization of xanthomonas euvesicatoria bacteriophages",
pages = "423-415",
number = "2",
volume = "93",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1051"
}

Gagić, K., Ivanović, M., Ignjatov, M., Ćalić, I.,& Obradović, A.. (2011). Isolation and characterization of xanthomonas euvesicatoria bacteriophages. in Journal of Plant Pathology, 93(2), 415-423.
https://hdl.handle.net/21.15107/rcub_fiver_1051

Gagić K, Ivanović M, Ignjatov M, Ćalić I, Obradović A. Isolation and characterization of xanthomonas euvesicatoria bacteriophages. in Journal of Plant Pathology. 2011;93(2):415-423.
https://hdl.handle.net/21.15107/rcub_fiver_1051 .

Gagić, K., Ivanović, Milan, Ignjatov, Maja, Ćalić, Irena, Obradović, Aleksa, "Isolation and characterization of xanthomonas euvesicatoria bacteriophages" in Journal of Plant Pathology, 93, no. 2 (2011):415-423,
https://hdl.handle.net/21.15107/rcub_fiver_1051 .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gašić, Katarina
AU  - Ivanović, Milan
AU  - Šević, Milan
AU  - Obradović, Aleksa
AU  - Milošević, Mirjana
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/782
AB  - During spring and summer of 2008, 101 bacterial strains was isolated from the diseased pepper leaves collected from different pepper growing areas in the Republic of Serbia. The aim of this research was to characterize the isolated strains and determine their taxonomic position according to the most recent nomenclature. Pathogenic, biochemical and physiological characteristics of isolated bacteria were tested using standard bacteriological tests. The pathogen races were determined according to the reaction of differential varieties of Early Calwonder (ECW), their isogenic lines (ECW-10R, ECW-20R, ECW-30R) and Capsicum pubescens. The sensitivity of strains to bactericides was studied in vitro by culturing bacteria on sucrose pepton agar (SPA) plates, amended with filter-sterilized aqueous solution of streptomycin and kasugamycin (50, 100, 200 ppm) or copper-sulphate (100, 200 ppm). Based on pathogenic, biochemical and physiological characteristics, the investigated strains belonged to Xanthomonas euvesicatoria. The reaction of pepper differential varieties indicated that these strains belonged to pepper races P1, P3, P7, P8. Streptomycin resistant strains were not detected, but 6 strains were resistant to kasugamycin (50 ppm) and 13 strains to copper-sulphate (200 ppm), indicating bacterial resistance development.
AB  - Tokom 2008. godine prikupljeni su uzorci obolelog lišća paprike sa simptomima bakteriozne pegavosti iz različitih lokaliteta Republike Srbije. Izolacijom iz zaraženih listova dobijen je 101 soj bakterija. Cilj ovog rada je proučavanje odlika i identifikacija izolovanih sojeva bakterije u skladu sa najnovijom nomenklaturom. Patogene i biohemijsko-fiziološke karakteristike sojeva proučene su korišćenjem standardnih bakterioloških testova. Određivanje fizioloških rasa bakterijske populacije izvršeno je na osnovu reakcije diferencijalnih sorti paprike Early Calwonder (ECW), njenih izogenih linija (ECW-10, ECW-20 ECW-30) i reakcijom sorte Capsicum pubescens. Proučena je osetljivost sojeva u in vitro uslovima na streptomicin, kasugamicin i bakar-sulfat. Rezultati proučavanja biohemijsko-fizioloških odlika sojeva ukazuju da naši sojevi pripadaju genetičkoj grupi 'A', odnosno vrsti Xanthomonas euvesicatoria. Proučavani sojevi, takođe, predstavljaju heterogenu populaciju u kojoj su zastupljene četiri fiziološke rase bakterije X. euvesicatoria (P1, P3, P7, P8). Sojevi rezistentni na streptomicin nisu detektovani ovim istraživanjima. Otpornost na 50 ppm kasugamicina utvrđena je kod 6 sojeva, a 13 sojeva je bilo otporno na 200 ppm bakar-sulfata. Prikazani rezultati ukazuju na opasnost od razvoja rezistentnosti bakterija na ova jedinjenja.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia
T1  - Karakterizacija sojeva Xanthomonas euvesicatoria, patogena paprike u Srbiji
EP  - 149
IS  - 2
SP  - 139
VL  - 25
DO  - 10.2298/PIF1002139I
ER  - 

@article{
author = "Ignjatov, Maja and Gašić, Katarina and Ivanović, Milan and Šević, Milan and Obradović, Aleksa and Milošević, Mirjana",
year = "2010",
abstract = "During spring and summer of 2008, 101 bacterial strains was isolated from the diseased pepper leaves collected from different pepper growing areas in the Republic of Serbia. The aim of this research was to characterize the isolated strains and determine their taxonomic position according to the most recent nomenclature. Pathogenic, biochemical and physiological characteristics of isolated bacteria were tested using standard bacteriological tests. The pathogen races were determined according to the reaction of differential varieties of Early Calwonder (ECW), their isogenic lines (ECW-10R, ECW-20R, ECW-30R) and Capsicum pubescens. The sensitivity of strains to bactericides was studied in vitro by culturing bacteria on sucrose pepton agar (SPA) plates, amended with filter-sterilized aqueous solution of streptomycin and kasugamycin (50, 100, 200 ppm) or copper-sulphate (100, 200 ppm). Based on pathogenic, biochemical and physiological characteristics, the investigated strains belonged to Xanthomonas euvesicatoria. The reaction of pepper differential varieties indicated that these strains belonged to pepper races P1, P3, P7, P8. Streptomycin resistant strains were not detected, but 6 strains were resistant to kasugamycin (50 ppm) and 13 strains to copper-sulphate (200 ppm), indicating bacterial resistance development., Tokom 2008. godine prikupljeni su uzorci obolelog lišća paprike sa simptomima bakteriozne pegavosti iz različitih lokaliteta Republike Srbije. Izolacijom iz zaraženih listova dobijen je 101 soj bakterija. Cilj ovog rada je proučavanje odlika i identifikacija izolovanih sojeva bakterije u skladu sa najnovijom nomenklaturom. Patogene i biohemijsko-fiziološke karakteristike sojeva proučene su korišćenjem standardnih bakterioloških testova. Određivanje fizioloških rasa bakterijske populacije izvršeno je na osnovu reakcije diferencijalnih sorti paprike Early Calwonder (ECW), njenih izogenih linija (ECW-10, ECW-20 ECW-30) i reakcijom sorte Capsicum pubescens. Proučena je osetljivost sojeva u in vitro uslovima na streptomicin, kasugamicin i bakar-sulfat. Rezultati proučavanja biohemijsko-fizioloških odlika sojeva ukazuju da naši sojevi pripadaju genetičkoj grupi 'A', odnosno vrsti Xanthomonas euvesicatoria. Proučavani sojevi, takođe, predstavljaju heterogenu populaciju u kojoj su zastupljene četiri fiziološke rase bakterije X. euvesicatoria (P1, P3, P7, P8). Sojevi rezistentni na streptomicin nisu detektovani ovim istraživanjima. Otpornost na 50 ppm kasugamicina utvrđena je kod 6 sojeva, a 13 sojeva je bilo otporno na 200 ppm bakar-sulfata. Prikazani rezultati ukazuju na opasnost od razvoja rezistentnosti bakterija na ova jedinjenja.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia, Karakterizacija sojeva Xanthomonas euvesicatoria, patogena paprike u Srbiji",
pages = "149-139",
number = "2",
volume = "25",
doi = "10.2298/PIF1002139I"
}

Ignjatov, M., Gašić, K., Ivanović, M., Šević, M., Obradović, A.,& Milošević, M.. (2010). Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 25(2), 139-149.
https://doi.org/10.2298/PIF1002139I

Ignjatov M, Gašić K, Ivanović M, Šević M, Obradović A, Milošević M. Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia. in Pesticidi i fitomedicina. 2010;25(2):139-149.
doi:10.2298/PIF1002139I .

Ignjatov, Maja, Gašić, Katarina, Ivanović, Milan, Šević, Milan, Obradović, Aleksa, Milošević, Mirjana, "Characterization of Xanthomonas euvesicatoria strains pathogens of pepper in Serbia" in Pesticidi i fitomedicina, 25, no. 2 (2010):139-149,
https://doi.org/10.2298/PIF1002139I . .