TY  - CONF
AU  - Yildirim, Kubilay
AU  - Kucuk, Ilkay Sevgen
AU  - Miladinović, Dragana
AU  - Saraç, Çigdem Gökcek
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/4024
AB  - Sesquiterpene lactones (STL) are a group of natural compounds found in various plant species, including sunflowers (Helianthus spp.), and they have been studied for their potential role in allelopathy and defense against pests and pathogens. Allelopathy refers to the ability of certain plants to release chemicals that affect the growth and development of neighboring plants and organisms. In the context of sunflower broomrape (Orobanche cumana) and sunflowers, STLs have been of particular interest due to their potential role in inhibiting the growth of the broomrape parasite. Some STLs have been shown to possess allelopathic properties, which means they can influence the germination and growth of other plants, potentially including parasitic plants like sunflower broomrape. Research has suggested that certain STLs found in sunflowers may exhibit inhibitory effects on the germination and growth of sunflower broomrape seeds. These compounds could potentially be released from the sunflower roots and into the soil, creating a hostile environment for the parasite. However, the effectiveness of sesquiterpene lactones in controlling sunflower broomrape is still an active area of research, and their practical application as a management strategy requires further investigation.
PB  - Edirne : Trakya University
C3  - Proceedings, 5th International Symposium on broomrape in sunflower, 1-3 November 2023, Antalya
T1  - Development CRISPR/CAS9-mediated resistance in sunflower against O. cumana
EP  - 25
SP  - 24
UR  - https://hdl.handle.net/21.15107/rcub_fiver_4024
ER  - 

@conference{
author = "Yildirim, Kubilay and Kucuk, Ilkay Sevgen and Miladinović, Dragana and Saraç, Çigdem Gökcek",
year = "2023",
abstract = "Sesquiterpene lactones (STL) are a group of natural compounds found in various plant species, including sunflowers (Helianthus spp.), and they have been studied for their potential role in allelopathy and defense against pests and pathogens. Allelopathy refers to the ability of certain plants to release chemicals that affect the growth and development of neighboring plants and organisms. In the context of sunflower broomrape (Orobanche cumana) and sunflowers, STLs have been of particular interest due to their potential role in inhibiting the growth of the broomrape parasite. Some STLs have been shown to possess allelopathic properties, which means they can influence the germination and growth of other plants, potentially including parasitic plants like sunflower broomrape. Research has suggested that certain STLs found in sunflowers may exhibit inhibitory effects on the germination and growth of sunflower broomrape seeds. These compounds could potentially be released from the sunflower roots and into the soil, creating a hostile environment for the parasite. However, the effectiveness of sesquiterpene lactones in controlling sunflower broomrape is still an active area of research, and their practical application as a management strategy requires further investigation.",
publisher = "Edirne : Trakya University",
journal = "Proceedings, 5th International Symposium on broomrape in sunflower, 1-3 November 2023, Antalya",
title = "Development CRISPR/CAS9-mediated resistance in sunflower against O. cumana",
pages = "25-24",
url = "https://hdl.handle.net/21.15107/rcub_fiver_4024"
}

Yildirim, K., Kucuk, I. S., Miladinović, D.,& Saraç, Ç. G.. (2023). Development CRISPR/CAS9-mediated resistance in sunflower against O. cumana. in Proceedings, 5th International Symposium on broomrape in sunflower, 1-3 November 2023, Antalya
Edirne : Trakya University., 24-25.
https://hdl.handle.net/21.15107/rcub_fiver_4024

Yildirim K, Kucuk IS, Miladinović D, Saraç ÇG. Development CRISPR/CAS9-mediated resistance in sunflower against O. cumana. in Proceedings, 5th International Symposium on broomrape in sunflower, 1-3 November 2023, Antalya. 2023;:24-25.
https://hdl.handle.net/21.15107/rcub_fiver_4024 .

Yildirim, Kubilay, Kucuk, Ilkay Sevgen, Miladinović, Dragana, Saraç, Çigdem Gökcek, "Development CRISPR/CAS9-mediated resistance in sunflower against O. cumana" in Proceedings, 5th International Symposium on broomrape in sunflower, 1-3 November 2023, Antalya (2023):24-25,
https://hdl.handle.net/21.15107/rcub_fiver_4024 .

TY  - CONF
AU  - Yildirim, Kubilay
AU  - Kucuk, Ilkay Sevgen
AU  - Kavas, Musa
AU  - Miladinović, Dragana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3757
AB  - Sunflower is one of the most important oil crops in the world that become a strategic plant due to the increased demand for its oil in recent years. Sunflower has low climate demand that enables it to grow in many regions of Europe. The biggest problem in sunflower cultivation is the presence of parasitic plants called broomrape (O. cumana). The seeds of these non-photosynthetic parasitic plants germinate with the secretion of Sesquiterpene Lactones (STL) from the roots of the sunflower. After attachment to the roots of the sunflower, it absorbs the water and all the nutrients from the host. Just one broomrape plant can produce millions of tiny seeds that can survive more than 15 years in the soil and can contaminate many fields in a region. Classical herbicides and mechanical techniques are not effective on these parasitic plants, since it already causes great damage to the plant when it rises above the ground. Many sunflower lines resistant to broomrape have been developed in last decades. However, the resistance of these lines was broken by emergence of new virulent broomrape strains. In recent years, secretion of Sesquiterpene Lactones (STLs) from sunflower roots has been found to trigger the germination of broomrape seeds. The genes encoding the enzymes (HaGAS, HaGAO, HaG8H, HaCOS) functional in STL biosynthesis in sunflower have been well characterized in recent years. In the light of all these information, genes of the enzymes that catalyze the production STLS was aimed to knockout with CRISPR/Cas9 technique in the project.
C3  - Book of Abstracts, 4th PlantEd Conference, Porto, 18-20 September 2023
T1  - Development of resistant sunflower lines to broomrape using CRISPR-CAS9
EP  - 53
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3757
ER  - 

@conference{
author = "Yildirim, Kubilay and Kucuk, Ilkay Sevgen and Kavas, Musa and Miladinović, Dragana",
year = "2023",
abstract = "Sunflower is one of the most important oil crops in the world that become a strategic plant due to the increased demand for its oil in recent years. Sunflower has low climate demand that enables it to grow in many regions of Europe. The biggest problem in sunflower cultivation is the presence of parasitic plants called broomrape (O. cumana). The seeds of these non-photosynthetic parasitic plants germinate with the secretion of Sesquiterpene Lactones (STL) from the roots of the sunflower. After attachment to the roots of the sunflower, it absorbs the water and all the nutrients from the host. Just one broomrape plant can produce millions of tiny seeds that can survive more than 15 years in the soil and can contaminate many fields in a region. Classical herbicides and mechanical techniques are not effective on these parasitic plants, since it already causes great damage to the plant when it rises above the ground. Many sunflower lines resistant to broomrape have been developed in last decades. However, the resistance of these lines was broken by emergence of new virulent broomrape strains. In recent years, secretion of Sesquiterpene Lactones (STLs) from sunflower roots has been found to trigger the germination of broomrape seeds. The genes encoding the enzymes (HaGAS, HaGAO, HaG8H, HaCOS) functional in STL biosynthesis in sunflower have been well characterized in recent years. In the light of all these information, genes of the enzymes that catalyze the production STLS was aimed to knockout with CRISPR/Cas9 technique in the project.",
journal = "Book of Abstracts, 4th PlantEd Conference, Porto, 18-20 September 2023",
title = "Development of resistant sunflower lines to broomrape using CRISPR-CAS9",
pages = "53-53",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3757"
}

Yildirim, K., Kucuk, I. S., Kavas, M.,& Miladinović, D.. (2023). Development of resistant sunflower lines to broomrape using CRISPR-CAS9. in Book of Abstracts, 4th PlantEd Conference, Porto, 18-20 September 2023, 53-53.
https://hdl.handle.net/21.15107/rcub_fiver_3757

Yildirim K, Kucuk IS, Kavas M, Miladinović D. Development of resistant sunflower lines to broomrape using CRISPR-CAS9. in Book of Abstracts, 4th PlantEd Conference, Porto, 18-20 September 2023. 2023;:53-53.
https://hdl.handle.net/21.15107/rcub_fiver_3757 .

Yildirim, Kubilay, Kucuk, Ilkay Sevgen, Kavas, Musa, Miladinović, Dragana, "Development of resistant sunflower lines to broomrape using CRISPR-CAS9" in Book of Abstracts, 4th PlantEd Conference, Porto, 18-20 September 2023 (2023):53-53,
https://hdl.handle.net/21.15107/rcub_fiver_3757 .

TY  - CONF
AU  - Yildirim, Kubilay
AU  - Sevgen, İlkay
AU  - Kondić-Špika, Ankica
AU  - Cvejić, Sandra
AU  - Jocić, Siniša
AU  - Miladinović, Dragana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2915
AB  - In recent years, sunflower has become a strategic plant due to the increased demand for its oil, as well as low input requirements that enable it to be grown in different agroecological conditions. The most important problem affecting sunflower yield and production are different biotic stressors, such as fungal pathogens and parasitic weed broomrape. The most effective and ecologically-friendly method of control of those stressors is creation of resistant genotypes. Novel, CRISPR-based techniques provide new tools that could accelerate breeding process and introduction of desirable traits into cultivated sunflower. In this work, researchers from Ondokuz Mayıs University and Institute of Field and Vegetable Crops (IFVCNS) combined their knowledge and know-how in order to develop effi cient CRISPR-based protocol(s) applicable in targeted improvement of sunflower.
PB  - Paris : International Sunflower Association
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia
T1  - The first report on efficient CRISPR-based protocol for sunflower
EP  - 186
SP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2915
ER  - 

@conference{
author = "Yildirim, Kubilay and Sevgen, İlkay and Kondić-Špika, Ankica and Cvejić, Sandra and Jocić, Siniša and Miladinović, Dragana",
year = "2022",
abstract = "In recent years, sunflower has become a strategic plant due to the increased demand for its oil, as well as low input requirements that enable it to be grown in different agroecological conditions. The most important problem affecting sunflower yield and production are different biotic stressors, such as fungal pathogens and parasitic weed broomrape. The most effective and ecologically-friendly method of control of those stressors is creation of resistant genotypes. Novel, CRISPR-based techniques provide new tools that could accelerate breeding process and introduction of desirable traits into cultivated sunflower. In this work, researchers from Ondokuz Mayıs University and Institute of Field and Vegetable Crops (IFVCNS) combined their knowledge and know-how in order to develop effi cient CRISPR-based protocol(s) applicable in targeted improvement of sunflower.",
publisher = "Paris : International Sunflower Association, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia",
title = "The first report on efficient CRISPR-based protocol for sunflower",
pages = "186-186",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2915"
}

Yildirim, K., Sevgen, İ., Kondić-Špika, A., Cvejić, S., Jocić, S.,& Miladinović, D.. (2022). The first report on efficient CRISPR-based protocol for sunflower. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia
Paris : International Sunflower Association., 186-186.
https://hdl.handle.net/21.15107/rcub_fiver_2915

Yildirim K, Sevgen İ, Kondić-Špika A, Cvejić S, Jocić S, Miladinović D. The first report on efficient CRISPR-based protocol for sunflower. in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia. 2022;:186-186.
https://hdl.handle.net/21.15107/rcub_fiver_2915 .

Yildirim, Kubilay, Sevgen, İlkay, Kondić-Špika, Ankica, Cvejić, Sandra, Jocić, Siniša, Miladinović, Dragana, "The first report on efficient CRISPR-based protocol for sunflower" in Proceedings, 20th International Sunflower Conference, 20-23 June 2022, Novi Sad, Serbia (2022):186-186,
https://hdl.handle.net/21.15107/rcub_fiver_2915 .

TY  - JOUR
AU  - Miladinović, Dragana
AU  - Antunes, Dulce
AU  - Yildirim, Kubilay
AU  - Bakhsh, Allah
AU  - Cvejić, Sandra
AU  - Kondić-Špika, Ankica
AU  - Marjanović-Jeromela, Ana
AU  - Opsahl-Sorteberg, Hilde-Gunn
AU  - Zambounis, Antonios
AU  - Hilioti, Zoe
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/6
AB  - Key message This review illustrates how far we have come since the emergence of GE technologies and how they could be applied to obtain superior and sustainable crop production. The main challenges of today's agriculture are maintaining and raising productivity, reducing its negative impact on the environment, and adapting to climate change. Efficient plant breeding can generate elite varieties that will rapidly replace obsolete ones and address ongoing challenges in an efficient and sustainable manner. Site-specific genome editing in plants is a rapidly evolving field with tangible results. The technology is equipped with a powerful toolbox of molecular scissors to cut DNA at a pre-determined site with different efficiencies for designing an approach that best suits the objectives of each plant breeding strategy. Genome editing (GE) not only revolutionizes plant biology, but provides the means to solve challenges related to plant architecture, food security, nutrient content, adaptation to the environment, resistance to diseases and production of plant-based materials. This review illustrates how far we have come since the emergence of these technologies and how these technologies could be applied to obtain superior, safe and sustainable crop production. Synergies of genome editing with other technological platforms that are gaining significance in plants lead to an exciting new, post-genomic era for plant research and production. In previous months, we have seen what global changes might arise from one new virus, reminding us of what drastic effects such events could have on food production. This demonstrates how important science, technology, and tools are to meet the current time and the future. Plant GE can make a real difference to future sustainable food production to the benefit of both mankind and our environment.
PB  - Springer, New York
T2  - Plant Cell Reports
T1  - Targeted plant improvement through genome editing: from laboratory to field
DO  - 10.1007/s00299-020-02655-4
ER  - 

@article{
author = "Miladinović, Dragana and Antunes, Dulce and Yildirim, Kubilay and Bakhsh, Allah and Cvejić, Sandra and Kondić-Špika, Ankica and Marjanović-Jeromela, Ana and Opsahl-Sorteberg, Hilde-Gunn and Zambounis, Antonios and Hilioti, Zoe",
year = "2021",
abstract = "Key message This review illustrates how far we have come since the emergence of GE technologies and how they could be applied to obtain superior and sustainable crop production. The main challenges of today's agriculture are maintaining and raising productivity, reducing its negative impact on the environment, and adapting to climate change. Efficient plant breeding can generate elite varieties that will rapidly replace obsolete ones and address ongoing challenges in an efficient and sustainable manner. Site-specific genome editing in plants is a rapidly evolving field with tangible results. The technology is equipped with a powerful toolbox of molecular scissors to cut DNA at a pre-determined site with different efficiencies for designing an approach that best suits the objectives of each plant breeding strategy. Genome editing (GE) not only revolutionizes plant biology, but provides the means to solve challenges related to plant architecture, food security, nutrient content, adaptation to the environment, resistance to diseases and production of plant-based materials. This review illustrates how far we have come since the emergence of these technologies and how these technologies could be applied to obtain superior, safe and sustainable crop production. Synergies of genome editing with other technological platforms that are gaining significance in plants lead to an exciting new, post-genomic era for plant research and production. In previous months, we have seen what global changes might arise from one new virus, reminding us of what drastic effects such events could have on food production. This demonstrates how important science, technology, and tools are to meet the current time and the future. Plant GE can make a real difference to future sustainable food production to the benefit of both mankind and our environment.",
publisher = "Springer, New York",
journal = "Plant Cell Reports",
title = "Targeted plant improvement through genome editing: from laboratory to field",
doi = "10.1007/s00299-020-02655-4"
}

Miladinović, D., Antunes, D., Yildirim, K., Bakhsh, A., Cvejić, S., Kondić-Špika, A., Marjanović-Jeromela, A., Opsahl-Sorteberg, H., Zambounis, A.,& Hilioti, Z.. (2021). Targeted plant improvement through genome editing: from laboratory to field. in Plant Cell Reports
Springer, New York..
https://doi.org/10.1007/s00299-020-02655-4

Miladinović D, Antunes D, Yildirim K, Bakhsh A, Cvejić S, Kondić-Špika A, Marjanović-Jeromela A, Opsahl-Sorteberg H, Zambounis A, Hilioti Z. Targeted plant improvement through genome editing: from laboratory to field. in Plant Cell Reports. 2021;.
doi:10.1007/s00299-020-02655-4 .

Miladinović, Dragana, Antunes, Dulce, Yildirim, Kubilay, Bakhsh, Allah, Cvejić, Sandra, Kondić-Špika, Ankica, Marjanović-Jeromela, Ana, Opsahl-Sorteberg, Hilde-Gunn, Zambounis, Antonios, Hilioti, Zoe, "Targeted plant improvement through genome editing: from laboratory to field" in Plant Cell Reports (2021),
https://doi.org/10.1007/s00299-020-02655-4 . .