TY  - JOUR
AU  - Aćimović, Milica
AU  - Šeregelj, Vanja
AU  - Simić, Katarina
AU  - Varga, Ana
AU  - Pezo, Lato
AU  - Vulić, Jelena
AU  - Čabarkapa, Ivana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3174
AB  - Essential  oil  (EO)  obtained  by  hydrodistillation  in  a  Clevenger-type  apparatus  from  aerial  parts  of  Nepeta cataria  L.  var.  citriodora (Becker), cultivated in Serbia was subjected to gas chromatography-mass spectroscopy (GC-MS) to determine the composition. Furthermore, N. cataria var. citriodora essential oil was tested to determine its antimicrobial, antioxidant, antihyperglycemic and anti-inflammatory activities in vitro. The antimicrobial activity was tested by broth microdilution method against 16 bacterial strains from American Type Culture Collection (ATCC). Four common tests for measuring in vitro antioxidant activity were used: 2, 2-diphenyl-1-picrylhydrazyl assay (DPPH), reducing power (RP), 2,2-azino-bis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) and β-carotene bleaching assay (BCB). Antihyperglycemic activity was examined by using α-glucosidase inhibitory potential (AHgA), while anti-inflammatory activity (AIA) was determined by protein denaturation bioassay, using egg albumin. In total, 36 compounds were isolated and detected by GC-MS technique in N. cataria var. citriodora EO. The EO is mainly comprised of oxygenated monoterpenes (93.1%), and the main compounds were two monoterpenoid alcohols, nerol (38.5%) and geraniol (24.9%), followed by two aliphatic aldehyde, geranial (14.6%) and neral (11.0%). Antimicrobial activity of this EO shows growth inhibition of all tested bacteria strains, and exhibited good antioxidant, antihyper-glycemic and anti-inflammatory activities. The EO obtained from N. cataria var. citriodora grown in Serbia shows valuable biological activity, indicating its potential for use as a supplement in everyday diet and as a natural preservative in food industry.
PB  - Lublin : University of Life Sciences
T2  - Acta Scientiarum Polonorum Hortorum Cultus
T1  - Chemical profile of Nepeta cataria L. var. Citriodora (Becker) essential oil and in vitro evaluation of biological activities
EP  - 74
IS  - 4
SP  - 67
VL  - 21
DO  - 10.24326/asphc.2022.4.7
ER  - 

@article{
author = "Aćimović, Milica and Šeregelj, Vanja and Simić, Katarina and Varga, Ana and Pezo, Lato and Vulić, Jelena and Čabarkapa, Ivana",
year = "2022",
abstract = "Essential  oil  (EO)  obtained  by  hydrodistillation  in  a  Clevenger-type  apparatus  from  aerial  parts  of  Nepeta cataria  L.  var.  citriodora (Becker), cultivated in Serbia was subjected to gas chromatography-mass spectroscopy (GC-MS) to determine the composition. Furthermore, N. cataria var. citriodora essential oil was tested to determine its antimicrobial, antioxidant, antihyperglycemic and anti-inflammatory activities in vitro. The antimicrobial activity was tested by broth microdilution method against 16 bacterial strains from American Type Culture Collection (ATCC). Four common tests for measuring in vitro antioxidant activity were used: 2, 2-diphenyl-1-picrylhydrazyl assay (DPPH), reducing power (RP), 2,2-azino-bis-3-ethylbenzo-thiazoline-6-sulfonic acid (ABTS) and β-carotene bleaching assay (BCB). Antihyperglycemic activity was examined by using α-glucosidase inhibitory potential (AHgA), while anti-inflammatory activity (AIA) was determined by protein denaturation bioassay, using egg albumin. In total, 36 compounds were isolated and detected by GC-MS technique in N. cataria var. citriodora EO. The EO is mainly comprised of oxygenated monoterpenes (93.1%), and the main compounds were two monoterpenoid alcohols, nerol (38.5%) and geraniol (24.9%), followed by two aliphatic aldehyde, geranial (14.6%) and neral (11.0%). Antimicrobial activity of this EO shows growth inhibition of all tested bacteria strains, and exhibited good antioxidant, antihyper-glycemic and anti-inflammatory activities. The EO obtained from N. cataria var. citriodora grown in Serbia shows valuable biological activity, indicating its potential for use as a supplement in everyday diet and as a natural preservative in food industry.",
publisher = "Lublin : University of Life Sciences",
journal = "Acta Scientiarum Polonorum Hortorum Cultus",
title = "Chemical profile of Nepeta cataria L. var. Citriodora (Becker) essential oil and in vitro evaluation of biological activities",
pages = "74-67",
number = "4",
volume = "21",
doi = "10.24326/asphc.2022.4.7"
}

Aćimović, M., Šeregelj, V., Simić, K., Varga, A., Pezo, L., Vulić, J.,& Čabarkapa, I.. (2022). Chemical profile of Nepeta cataria L. var. Citriodora (Becker) essential oil and in vitro evaluation of biological activities. in Acta Scientiarum Polonorum Hortorum Cultus
Lublin : University of Life Sciences., 21(4), 67-74.
https://doi.org/10.24326/asphc.2022.4.7

Aćimović M, Šeregelj V, Simić K, Varga A, Pezo L, Vulić J, Čabarkapa I. Chemical profile of Nepeta cataria L. var. Citriodora (Becker) essential oil and in vitro evaluation of biological activities. in Acta Scientiarum Polonorum Hortorum Cultus. 2022;21(4):67-74.
doi:10.24326/asphc.2022.4.7 .

Aćimović, Milica, Šeregelj, Vanja, Simić, Katarina, Varga, Ana, Pezo, Lato, Vulić, Jelena, Čabarkapa, Ivana, "Chemical profile of Nepeta cataria L. var. Citriodora (Becker) essential oil and in vitro evaluation of biological activities" in Acta Scientiarum Polonorum Hortorum Cultus, 21, no. 4 (2022):67-74,
https://doi.org/10.24326/asphc.2022.4.7 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Šovljanski, Olja
AU  - Šeregelj, Vanja
AU  - Pezo, Lato
AU  - Zheljazkov, Valtcho
AU  - Ljujić, Jovana
AU  - Tomić, Ana
AU  - Ćetković, Gordana
AU  - Čanadanović-Brunet, Jasna
AU  - Miljković, Ana
AU  - Vujisić, Ljubodrag
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2564
AB  - Steam distillation was used for the isolation of Dracocephalum moldavica L. (Moldavian dragonhead) essential oil (DMEO). This aromatic herbaceous plant is widespread across the Northern Hemisphere regions and has been utilized in health-improving studies and applications. In addition to the DMEO, the hydrolate (DMH), a byproduct of the distillation process, was also collected. The DMEO and DMH were analyzed and compared in terms of their chemical composition, as well as their in vitro biological activities. The main component in DMEO was geranyl acetate, while geranial was dominant in DMH. The DMEO demonstrated better antioxidant and antimicrobial activities compared with the DMH against Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, and Listeria monocytogenes, which represent sources of food-borne illness at the global level. The DMEO and DMH show promise as antioxidant and antimicrobial additives to various products.
PB  - Basel : MDPI
T2  - Plants (Basel)
T1  - Chemical composition, antioxidant, and antimicrobial activity of Dracocephalum moldavica L. essential oil and hydrolate
IS  - 7
SP  - 941
VL  - 11
DO  - 10.3390/plants11070941
ER  - 

@article{
author = "Aćimović, Milica and Šovljanski, Olja and Šeregelj, Vanja and Pezo, Lato and Zheljazkov, Valtcho and Ljujić, Jovana and Tomić, Ana and Ćetković, Gordana and Čanadanović-Brunet, Jasna and Miljković, Ana and Vujisić, Ljubodrag",
year = "2022",
abstract = "Steam distillation was used for the isolation of Dracocephalum moldavica L. (Moldavian dragonhead) essential oil (DMEO). This aromatic herbaceous plant is widespread across the Northern Hemisphere regions and has been utilized in health-improving studies and applications. In addition to the DMEO, the hydrolate (DMH), a byproduct of the distillation process, was also collected. The DMEO and DMH were analyzed and compared in terms of their chemical composition, as well as their in vitro biological activities. The main component in DMEO was geranyl acetate, while geranial was dominant in DMH. The DMEO demonstrated better antioxidant and antimicrobial activities compared with the DMH against Staphylococcus aureus, Escherichia coli, Salmonella Typhimurium, and Listeria monocytogenes, which represent sources of food-borne illness at the global level. The DMEO and DMH show promise as antioxidant and antimicrobial additives to various products.",
publisher = "Basel : MDPI",
journal = "Plants (Basel)",
title = "Chemical composition, antioxidant, and antimicrobial activity of Dracocephalum moldavica L. essential oil and hydrolate",
number = "7",
pages = "941",
volume = "11",
doi = "10.3390/plants11070941"
}

Aćimović, M., Šovljanski, O., Šeregelj, V., Pezo, L., Zheljazkov, V., Ljujić, J., Tomić, A., Ćetković, G., Čanadanović-Brunet, J., Miljković, A.,& Vujisić, L.. (2022). Chemical composition, antioxidant, and antimicrobial activity of Dracocephalum moldavica L. essential oil and hydrolate. in Plants (Basel)
Basel : MDPI., 11(7), 941.
https://doi.org/10.3390/plants11070941

Aćimović M, Šovljanski O, Šeregelj V, Pezo L, Zheljazkov V, Ljujić J, Tomić A, Ćetković G, Čanadanović-Brunet J, Miljković A, Vujisić L. Chemical composition, antioxidant, and antimicrobial activity of Dracocephalum moldavica L. essential oil and hydrolate. in Plants (Basel). 2022;11(7):941.
doi:10.3390/plants11070941 .

Aćimović, Milica, Šovljanski, Olja, Šeregelj, Vanja, Pezo, Lato, Zheljazkov, Valtcho, Ljujić, Jovana, Tomić, Ana, Ćetković, Gordana, Čanadanović-Brunet, Jasna, Miljković, Ana, Vujisić, Ljubodrag, "Chemical composition, antioxidant, and antimicrobial activity of Dracocephalum moldavica L. essential oil and hydrolate" in Plants (Basel), 11, no. 7 (2022):941,
https://doi.org/10.3390/plants11070941 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Šeregelj, Vanja
AU  - Šovljanski, Olja
AU  - Tumbas Šaponjac, Vesna
AU  - Švarc Gajić, Jaroslava
AU  - Brezo-Borjan, Tanja
AU  - Pezo, Lato
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2196
AB  - As the main goal of this study was the verification of Satureja kitaibelii Wierzb. ex Heuff. in form of the crude extract obtained by subcritical water extraction (SWE) technique in terms of polyphenolic composition, in vitro antioxidant capacity, antihyperglycemic, and anti-inflammatory activities as well as an antimicrobial effect against 10 microorganisms. HPLC analysis showed the presence of ten compounds in a total concentration of 89.33 mg/g of the SWE extract, and demonstrated that subcritical water polarity was the most selective for extracting syringic and caffeic acid, as well as epicatechin (37.88 18.06, and 10.04 mg/g, respectively), while flavonoids like rutin, luteolin, and apigenin were present in low concentration (<2 mg/g). Results of antioxidant activity assays revealed that superoxide anion and ABTS•+ radical models expressed the highest scavenging abilities (437.35 and 412.09 μmol TE/g, respectively), followed by lipid radicals (69.61 μmol TE/g), and DPPH• (32.28 μmol TE/g), while in the case of ferric ion reducing ability was noted a high value (118.19 μmol TE/g). S. kitaibelii SWE was less efficient in the antihyperglycemic assay (AhgA = 30.72 %), but the high antiinflammatory activity of S. kitaibelii SWE was observed (30.35 %). The antimicrobial activity was demonstrated against Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes as well as Saccharomyces cerevisiae, Candida albicans, and Aspergillus brasiliensis through antimicrobial screening, minimal inhibitory concentration, and pharmacodynamics study.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract
SP  - 113672
VL  - 169
DO  - 10.1016/j.indcrop.2021.113672
ER  - 

@article{
author = "Aćimović, Milica and Šeregelj, Vanja and Šovljanski, Olja and Tumbas Šaponjac, Vesna and Švarc Gajić, Jaroslava and Brezo-Borjan, Tanja and Pezo, Lato",
year = "2021",
abstract = "As the main goal of this study was the verification of Satureja kitaibelii Wierzb. ex Heuff. in form of the crude extract obtained by subcritical water extraction (SWE) technique in terms of polyphenolic composition, in vitro antioxidant capacity, antihyperglycemic, and anti-inflammatory activities as well as an antimicrobial effect against 10 microorganisms. HPLC analysis showed the presence of ten compounds in a total concentration of 89.33 mg/g of the SWE extract, and demonstrated that subcritical water polarity was the most selective for extracting syringic and caffeic acid, as well as epicatechin (37.88 18.06, and 10.04 mg/g, respectively), while flavonoids like rutin, luteolin, and apigenin were present in low concentration (<2 mg/g). Results of antioxidant activity assays revealed that superoxide anion and ABTS•+ radical models expressed the highest scavenging abilities (437.35 and 412.09 μmol TE/g, respectively), followed by lipid radicals (69.61 μmol TE/g), and DPPH• (32.28 μmol TE/g), while in the case of ferric ion reducing ability was noted a high value (118.19 μmol TE/g). S. kitaibelii SWE was less efficient in the antihyperglycemic assay (AhgA = 30.72 %), but the high antiinflammatory activity of S. kitaibelii SWE was observed (30.35 %). The antimicrobial activity was demonstrated against Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes as well as Saccharomyces cerevisiae, Candida albicans, and Aspergillus brasiliensis through antimicrobial screening, minimal inhibitory concentration, and pharmacodynamics study.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract",
pages = "113672",
volume = "169",
doi = "10.1016/j.indcrop.2021.113672"
}

Aćimović, M., Šeregelj, V., Šovljanski, O., Tumbas Šaponjac, V., Švarc Gajić, J., Brezo-Borjan, T.,& Pezo, L.. (2021). In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract. in Industrial Crops and Products
Elsevier., 169, 113672.
https://doi.org/10.1016/j.indcrop.2021.113672

Aćimović M, Šeregelj V, Šovljanski O, Tumbas Šaponjac V, Švarc Gajić J, Brezo-Borjan T, Pezo L. In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract. in Industrial Crops and Products. 2021;169:113672.
doi:10.1016/j.indcrop.2021.113672 .

Aćimović, Milica, Šeregelj, Vanja, Šovljanski, Olja, Tumbas Šaponjac, Vesna, Švarc Gajić, Jaroslava, Brezo-Borjan, Tanja, Pezo, Lato, "In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract" in Industrial Crops and Products, 169 (2021):113672,
https://doi.org/10.1016/j.indcrop.2021.113672 . .

TY  - CONF
AU  - Špirić, Nemanja
AU  - Šeregelj, Vanja
AU  - Tumbas, Vesna
AU  - Šovljanski, Olja
AU  - Tomić, Ana
AU  - Aćimović, Milica
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2346
AB  - Satureja kitaibelii Wierzb. ex Heuff. or Rtanj tea is an endemic species of the Carpathian
mountain chain on the Balkan Peninsula. It grows in Eastern Serbia, Northwest Bulgaria, and
Southwest Romania. As traditional remedy, it is used for treatment of different stomach
discomforts such as pains in the lower abdomen, upset stomach, diarrhoea and nausea, for the
regulation of the menstrual cycle and fertility, for muscle relaxation, and in the treatment of the
upper respiratory tract infections. Therefore, the aim of this research was to examine the
phytochemical composition and antioxidant activity of S. kitaibelii in form of subcritical water
extract (SWE). HPLC analysis of SWE of S. kitaibelii showed the presence of ten compounds in
a total concentration of 89.33 mg/g of the extract. It was demonstrated that the polarity of
subcritical water was the most selective for extracting syringic acid (37.88 mg/g), caffeic acid
(18.06 mg/g), and epicatechin (10.04 mg/g). Flavonoids like rutin, luteolin, and apigenin were the
least abundant in this extract (<2 mg/g). Additionally, this study investigated in vitro antioxidant
activities of S. kitaibelii SWE by five antioxidant assays. Four assays present radical scavenging
activity while the last assay indicates transitional metal reduction. The highest scavenging
abilities were observed against superoxide anion and ABTS•+ radicals (437.35 and 412.09 μmol
TE/g), followed by lipid radicals (69.61 μmol TE/g), and DPPH• (32.28 μmol TE/g).
PB  - East Sarajevo : Faculty of Agriculture
C3  - Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021
T1  - In vitro antioxidant activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract
EP  - 234
SP  - 234
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2346
ER  - 

@conference{
author = "Špirić, Nemanja and Šeregelj, Vanja and Tumbas, Vesna and Šovljanski, Olja and Tomić, Ana and Aćimović, Milica",
year = "2021",
abstract = "Satureja kitaibelii Wierzb. ex Heuff. or Rtanj tea is an endemic species of the Carpathian
mountain chain on the Balkan Peninsula. It grows in Eastern Serbia, Northwest Bulgaria, and
Southwest Romania. As traditional remedy, it is used for treatment of different stomach
discomforts such as pains in the lower abdomen, upset stomach, diarrhoea and nausea, for the
regulation of the menstrual cycle and fertility, for muscle relaxation, and in the treatment of the
upper respiratory tract infections. Therefore, the aim of this research was to examine the
phytochemical composition and antioxidant activity of S. kitaibelii in form of subcritical water
extract (SWE). HPLC analysis of SWE of S. kitaibelii showed the presence of ten compounds in
a total concentration of 89.33 mg/g of the extract. It was demonstrated that the polarity of
subcritical water was the most selective for extracting syringic acid (37.88 mg/g), caffeic acid
(18.06 mg/g), and epicatechin (10.04 mg/g). Flavonoids like rutin, luteolin, and apigenin were the
least abundant in this extract (<2 mg/g). Additionally, this study investigated in vitro antioxidant
activities of S. kitaibelii SWE by five antioxidant assays. Four assays present radical scavenging
activity while the last assay indicates transitional metal reduction. The highest scavenging
abilities were observed against superoxide anion and ABTS•+ radicals (437.35 and 412.09 μmol
TE/g), followed by lipid radicals (69.61 μmol TE/g), and DPPH• (32.28 μmol TE/g).",
publisher = "East Sarajevo : Faculty of Agriculture",
journal = "Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021",
title = "In vitro antioxidant activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract",
pages = "234-234",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2346"
}

Špirić, N., Šeregelj, V., Tumbas, V., Šovljanski, O., Tomić, A.,& Aćimović, M.. (2021). In vitro antioxidant activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract. in Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021
East Sarajevo : Faculty of Agriculture., 234-234.
https://hdl.handle.net/21.15107/rcub_fiver_2346

Špirić N, Šeregelj V, Tumbas V, Šovljanski O, Tomić A, Aćimović M. In vitro antioxidant activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract. in Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021. 2021;:234-234.
https://hdl.handle.net/21.15107/rcub_fiver_2346 .

Špirić, Nemanja, Šeregelj, Vanja, Tumbas, Vesna, Šovljanski, Olja, Tomić, Ana, Aćimović, Milica, "In vitro antioxidant activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract" in Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021 (2021):234-234,
https://hdl.handle.net/21.15107/rcub_fiver_2346 .