Jovanović, Živko

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orcid::0000-0003-1626-1531
  • Jovanović, Živko (13)
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Author's Bibliography

Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains

Lozo, Jelena; Danojević, Dario; Jovanović, Živko; Nenadović, Željka; Fira, Đorđe; Stanković, Slaviša; Radović, Svetlana

(Basel : MDPI, 2022)

TY  - JOUR
AU  - Lozo, Jelena
AU  - Danojević, Dario
AU  - Jovanović, Živko
AU  - Nenadović, Željka
AU  - Fira, Đorđe
AU  - Stanković, Slaviša
AU  - Radović, Svetlana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2653
AB  - We examined the effect of drought-tolerant Bacillus safensis SS-2.7 and B. thuringiensis SS-29.2 strains on the response of four (133, 274, California Wonder—CalW, and Matica) sweet pepper genotypes to water deficiency conditions. Pepper seeds were sown in pots with (treated) and without (control) bacterial strain inoculation. After four weeks of growth under controlled conditions and regular watering, drought was imposed by completely withholding watering for seven days. Under conditions of normal watering, genotype 274 showed better seedling establishment than genotype 133 and CalW, while the slowest was genotype Matica. Antioxidant enzyme activity under drought conditions was genotype and bacterial treatment-dependent. The best response to bacterial treatment in order to cope with severe drought was found in the CalW genotype, while in genotype 133, we determined even faster plant decay during water deficiency in treated seeds. Inoculated seeds of the Matica genotype did not show different antioxidant enzyme activity under normal and drought conditions. According to the obtained results, we concluded that under drought conditions, the most susceptible was genotype 274, moderate susceptibility was detected in genotype 133, and CalW and Matica were the most tolerant genotypes. Our study demonstrates (1) that drought-tolerant Bacillus strains showed a plant growth-promoting effect on some selected pepper genotypes; (2) that there were genotype-dependent antioxidant enzyme activities under drought conditions in response to treatment with a particular bacterial strain; and (3) that we could expect a genotype-dependent response during biostimulant application, especially under stress conditions.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains
SP  - 236
VL  - 8
DO  - 10.3390/horticulturae8030236
ER  - 
@article{
author = "Lozo, Jelena and Danojević, Dario and Jovanović, Živko and Nenadović, Željka and Fira, Đorđe and Stanković, Slaviša and Radović, Svetlana",
year = "2022",
abstract = "We examined the effect of drought-tolerant Bacillus safensis SS-2.7 and B. thuringiensis SS-29.2 strains on the response of four (133, 274, California Wonder—CalW, and Matica) sweet pepper genotypes to water deficiency conditions. Pepper seeds were sown in pots with (treated) and without (control) bacterial strain inoculation. After four weeks of growth under controlled conditions and regular watering, drought was imposed by completely withholding watering for seven days. Under conditions of normal watering, genotype 274 showed better seedling establishment than genotype 133 and CalW, while the slowest was genotype Matica. Antioxidant enzyme activity under drought conditions was genotype and bacterial treatment-dependent. The best response to bacterial treatment in order to cope with severe drought was found in the CalW genotype, while in genotype 133, we determined even faster plant decay during water deficiency in treated seeds. Inoculated seeds of the Matica genotype did not show different antioxidant enzyme activity under normal and drought conditions. According to the obtained results, we concluded that under drought conditions, the most susceptible was genotype 274, moderate susceptibility was detected in genotype 133, and CalW and Matica were the most tolerant genotypes. Our study demonstrates (1) that drought-tolerant Bacillus strains showed a plant growth-promoting effect on some selected pepper genotypes; (2) that there were genotype-dependent antioxidant enzyme activities under drought conditions in response to treatment with a particular bacterial strain; and (3) that we could expect a genotype-dependent response during biostimulant application, especially under stress conditions.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains",
pages = "236",
volume = "8",
doi = "10.3390/horticulturae8030236"
}
Lozo, J., Danojević, D., Jovanović, Ž., Nenadović, Ž., Fira, Đ., Stanković, S.,& Radović, S.. (2022). Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains. in Horticulturae
Basel : MDPI., 8, 236.
https://doi.org/10.3390/horticulturae8030236
Lozo J, Danojević D, Jovanović Ž, Nenadović Ž, Fira Đ, Stanković S, Radović S. Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains. in Horticulturae. 2022;8:236.
doi:10.3390/horticulturae8030236 .
Lozo, Jelena, Danojević, Dario, Jovanović, Živko, Nenadović, Željka, Fira, Đorđe, Stanković, Slaviša, Radović, Svetlana, "Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains" in Horticulturae, 8 (2022):236,
https://doi.org/10.3390/horticulturae8030236 . .
2
6
5

Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains

Nenadović, Željka; Danojević, Dario; Jovanović, Živko; Stanković, Slaviša; Fira, Đorđe; Radović, Svetlana; Lozo, Jelena

(Belgrade : Serbian Genetic Society, 2019)

TY  - CONF
AU  - Nenadović, Željka
AU  - Danojević, Dario
AU  - Jovanović, Živko
AU  - Stanković, Slaviša
AU  - Fira, Đorđe
AU  - Radović, Svetlana
AU  - Lozo, Jelena
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3061
AB  - The worldwide increase in human population followed with industrialization led to environmental changes as a result of water, soil and air pollution. It is believed that one way to address this problem is to change the existing agricultural approaches and to use plant growth promoting bacteria (PGPB) instead of fertilizers and pesticides. The present investigation was aimed to evaluate the influence of PGPB on sweet pepper seed priming. Pepper seeds (Capsicum annuum L.) from six different genotype, 115 and 116 (peppers for spice), 261 (sivri type), 133, 258, and 274 (kapia type) were primed with 10-9 CFU/ml of Bacillus safensis (SS-2.7), B. thuringiensis (SS-29.2), Pseudomonas putida (4.3) and P. protegens CHA0 strains. Seeds were primed during one hour and after three weeks root and seedlings length, and the total mass of the pepper seedlings were analyzed. One and two-way analysis of variance (ANOVA) and Least Significant Difference test (LSD test) with a 0.05 probability level was used for statistical analysis.
PB  - Belgrade : Serbian Genetic Society
C3  - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
T1  - Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains
EP  - 274
SP  - 274
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3061
ER  - 
@conference{
author = "Nenadović, Željka and Danojević, Dario and Jovanović, Živko and Stanković, Slaviša and Fira, Đorđe and Radović, Svetlana and Lozo, Jelena",
year = "2019",
abstract = "The worldwide increase in human population followed with industrialization led to environmental changes as a result of water, soil and air pollution. It is believed that one way to address this problem is to change the existing agricultural approaches and to use plant growth promoting bacteria (PGPB) instead of fertilizers and pesticides. The present investigation was aimed to evaluate the influence of PGPB on sweet pepper seed priming. Pepper seeds (Capsicum annuum L.) from six different genotype, 115 and 116 (peppers for spice), 261 (sivri type), 133, 258, and 274 (kapia type) were primed with 10-9 CFU/ml of Bacillus safensis (SS-2.7), B. thuringiensis (SS-29.2), Pseudomonas putida (4.3) and P. protegens CHA0 strains. Seeds were primed during one hour and after three weeks root and seedlings length, and the total mass of the pepper seedlings were analyzed. One and two-way analysis of variance (ANOVA) and Least Significant Difference test (LSD test) with a 0.05 probability level was used for statistical analysis.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja",
title = "Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains",
pages = "274-274",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3061"
}
Nenadović, Ž., Danojević, D., Jovanović, Ž., Stanković, S., Fira, Đ., Radović, S.,& Lozo, J.. (2019). Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
Belgrade : Serbian Genetic Society., 274-274.
https://hdl.handle.net/21.15107/rcub_fiver_3061
Nenadović Ž, Danojević D, Jovanović Ž, Stanković S, Fira Đ, Radović S, Lozo J. Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:274-274.
https://hdl.handle.net/21.15107/rcub_fiver_3061 .
Nenadović, Željka, Danojević, Dario, Jovanović, Živko, Stanković, Slaviša, Fira, Đorđe, Radović, Svetlana, Lozo, Jelena, "Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):274-274,
https://hdl.handle.net/21.15107/rcub_fiver_3061 .

A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication

Smykal, Petr; Jovanović, Živko; Stanisavljević, Nemanja; Zlatković, Bojan; Ćupina, Branko; Đorđević, Vuk; Mikić, Aleksandar; Medović, Aleksandar

(Springer, Dordrecht, 2014)

TY  - JOUR
AU  - Smykal, Petr
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Zlatković, Bojan
AU  - Ćupina, Branko
AU  - Đorđević, Vuk
AU  - Mikić, Aleksandar
AU  - Medović, Aleksandar
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1380
AB  - The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.
PB  - Springer, Dordrecht
T2  - Genetic Resources & Crop Evolution
T1  - A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication
EP  - 1544
IS  - 8
SP  - 1533
VL  - 61
DO  - 10.1007/s10722-014-0128-z
ER  - 
@article{
author = "Smykal, Petr and Jovanović, Živko and Stanisavljević, Nemanja and Zlatković, Bojan and Ćupina, Branko and Đorđević, Vuk and Mikić, Aleksandar and Medović, Aleksandar",
year = "2014",
abstract = "The development of agriculture was a key turning point in human history, a central part of which was the evolution of new plant forms, domesticated crops. Grain legumes were domesticated in parallel with cereals and formed important dietary components of early civilizations. First domesticated in the Near East, pea has been cultivated in Europe since the Stone and Bronze Ages. In this study, we present a molecular analysis of ancient DNA (aDNA) extracted from carbonized pea seeds recovered from deposits at Hissar, in southeast Serbia, that date to the eleventh century B.C. Four selected chloroplast DNA loci (trnSG, trnK, matK and rbcL) amplified in six fragments of 128-340 bp with a total length of 1,329 bp were successfully recovered in order to distinguish between cultivated and wild gathered pea. Based on identified mutations, the results showed that genuine aDNA was analyzed. Moreover, DNA analysis resulted in placing the ancient sample at an intermediate position between extant cultivated [Pisum sativum L. and wild P. sativum subsp. elatius (Steven ex M. Bieb.) Asch. et Graebn.]. Consequently, based on a combination of morphological and molecular data, we concluded that the material represents an early domesticated pea. We speculate that Iron Age pea would be of colored flower and pigmented testa, similar to today's fodder pea (P. sativum subsp. sativum var. arvense (L.) Poir.), possibly of winter type. This is the first report of successful aDNA extraction and analysis from any legume species thus far. The implications for pea domestication are discussed here.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources & Crop Evolution",
title = "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication",
pages = "1544-1533",
number = "8",
volume = "61",
doi = "10.1007/s10722-014-0128-z"
}
Smykal, P., Jovanović, Ž., Stanisavljević, N., Zlatković, B., Ćupina, B., Đorđević, V., Mikić, A.,& Medović, A.. (2014). A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources & Crop Evolution
Springer, Dordrecht., 61(8), 1533-1544.
https://doi.org/10.1007/s10722-014-0128-z
Smykal P, Jovanović Ž, Stanisavljević N, Zlatković B, Ćupina B, Đorđević V, Mikić A, Medović A. A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication. in Genetic Resources & Crop Evolution. 2014;61(8):1533-1544.
doi:10.1007/s10722-014-0128-z .
Smykal, Petr, Jovanović, Živko, Stanisavljević, Nemanja, Zlatković, Bojan, Ćupina, Branko, Đorđević, Vuk, Mikić, Aleksandar, Medović, Aleksandar, "A comparative study of ancient DNA isolated from charred pea (Pisum sativum L.) seeds from an Early Iron Age settlement in southeast Serbia: inference for pea domestication" in Genetic Resources & Crop Evolution, 61, no. 8 (2014):1533-1544,
https://doi.org/10.1007/s10722-014-0128-z . .
21
13
20

Evaluation of seed yield and seed yield components in red-yellow (Pisum fulvum) and Ethiopian (Pisum abyssinicum) peas

Mikić, Aleksandar; Mihailović, Vojislav; Dimitrijević, Miodrag; Petrović, Sofija; Ćupina, Branko; Đorđević, Vuk; Kosev, Valentin; Milošević, Branko; Jovanović, Živko; Milovac, Željko

(Springer, Dordrecht, 2013)

TY  - JOUR
AU  - Mikić, Aleksandar
AU  - Mihailović, Vojislav
AU  - Dimitrijević, Miodrag
AU  - Petrović, Sofija
AU  - Ćupina, Branko
AU  - Đorđević, Vuk
AU  - Kosev, Valentin
AU  - Milošević, Branko
AU  - Jovanović, Živko
AU  - Milovac, Željko
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1274
AB  - Red-yellow (Pisum fulvum Sibth. et Sm.) and Ethiopian (Pisum abyssinicum A. Br.) peas have become of increasing interest to breeders in the last decade, as they have been found to be partially or completely tolerant to various biotic stresses, such as to attack by pea weevil, mildew blight or rust. A trial was carried out at the Institute of Field and Vegetable Crops at Rimski anevi from 2005 to 2007, with 13 accessions each of red-yellow and Ethiopian peas sown at 100 viable seeds m(-2), in plots of 0.5 m(2). Seed yield per plant in red-yellow pea was significantly and positively correlated with seed number per plant (r = 0.881**), pod number per plant (r = 0.839**) and number of fertile nodes (r = 0.820**). The highest positive correlation among the agronomic characteristics in Ethiopian pea was between number of fertile nodes and number of pods (r = 0.937**). Seed yield in Ethiopian pea was highly significantly correlated with number of seeds (r = 0.807**), pods (r = 0.692*), and fertile nodes (r = 0.638*). The results suggest that plant morphology of the progenies between red-yellow or Ethiopian peas and grain-type common pea could not differ significantly from that of individual parents. That means that it could be possible to develop hybrid lines that could keep the desirable morphological traits of grain-type common pea, such as lodging-tolerance and high seed yields, and to make an introgression of a specific resistance from wild pea taxa, especially by back-crosses with the former. However, one must always be aware of unpredicted outcomes as a result of rather different genetic basis of individual seed yield components.
PB  - Springer, Dordrecht
T2  - Genetic Resources & Crop Evolution
T1  - Evaluation of seed yield and seed yield components in red-yellow (Pisum fulvum) and Ethiopian (Pisum abyssinicum) peas
EP  - 638
IS  - 2
SP  - 629
VL  - 60
DO  - 10.1007/s10722-012-9862-2
ER  - 
@article{
author = "Mikić, Aleksandar and Mihailović, Vojislav and Dimitrijević, Miodrag and Petrović, Sofija and Ćupina, Branko and Đorđević, Vuk and Kosev, Valentin and Milošević, Branko and Jovanović, Živko and Milovac, Željko",
year = "2013",
abstract = "Red-yellow (Pisum fulvum Sibth. et Sm.) and Ethiopian (Pisum abyssinicum A. Br.) peas have become of increasing interest to breeders in the last decade, as they have been found to be partially or completely tolerant to various biotic stresses, such as to attack by pea weevil, mildew blight or rust. A trial was carried out at the Institute of Field and Vegetable Crops at Rimski anevi from 2005 to 2007, with 13 accessions each of red-yellow and Ethiopian peas sown at 100 viable seeds m(-2), in plots of 0.5 m(2). Seed yield per plant in red-yellow pea was significantly and positively correlated with seed number per plant (r = 0.881**), pod number per plant (r = 0.839**) and number of fertile nodes (r = 0.820**). The highest positive correlation among the agronomic characteristics in Ethiopian pea was between number of fertile nodes and number of pods (r = 0.937**). Seed yield in Ethiopian pea was highly significantly correlated with number of seeds (r = 0.807**), pods (r = 0.692*), and fertile nodes (r = 0.638*). The results suggest that plant morphology of the progenies between red-yellow or Ethiopian peas and grain-type common pea could not differ significantly from that of individual parents. That means that it could be possible to develop hybrid lines that could keep the desirable morphological traits of grain-type common pea, such as lodging-tolerance and high seed yields, and to make an introgression of a specific resistance from wild pea taxa, especially by back-crosses with the former. However, one must always be aware of unpredicted outcomes as a result of rather different genetic basis of individual seed yield components.",
publisher = "Springer, Dordrecht",
journal = "Genetic Resources & Crop Evolution",
title = "Evaluation of seed yield and seed yield components in red-yellow (Pisum fulvum) and Ethiopian (Pisum abyssinicum) peas",
pages = "638-629",
number = "2",
volume = "60",
doi = "10.1007/s10722-012-9862-2"
}
Mikić, A., Mihailović, V., Dimitrijević, M., Petrović, S., Ćupina, B., Đorđević, V., Kosev, V., Milošević, B., Jovanović, Ž.,& Milovac, Ž.. (2013). Evaluation of seed yield and seed yield components in red-yellow (Pisum fulvum) and Ethiopian (Pisum abyssinicum) peas. in Genetic Resources & Crop Evolution
Springer, Dordrecht., 60(2), 629-638.
https://doi.org/10.1007/s10722-012-9862-2
Mikić A, Mihailović V, Dimitrijević M, Petrović S, Ćupina B, Đorđević V, Kosev V, Milošević B, Jovanović Ž, Milovac Ž. Evaluation of seed yield and seed yield components in red-yellow (Pisum fulvum) and Ethiopian (Pisum abyssinicum) peas. in Genetic Resources & Crop Evolution. 2013;60(2):629-638.
doi:10.1007/s10722-012-9862-2 .
Mikić, Aleksandar, Mihailović, Vojislav, Dimitrijević, Miodrag, Petrović, Sofija, Ćupina, Branko, Đorđević, Vuk, Kosev, Valentin, Milošević, Branko, Jovanović, Živko, Milovac, Željko, "Evaluation of seed yield and seed yield components in red-yellow (Pisum fulvum) and Ethiopian (Pisum abyssinicum) peas" in Genetic Resources & Crop Evolution, 60, no. 2 (2013):629-638,
https://doi.org/10.1007/s10722-012-9862-2 . .
8
8
3
9

Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity

Stanisavljević, Nemanja; Vukotić, Goran; Fira, Đorđe; Jovanović, Živko; Miljuš-Đukić, Jovanka; Radović, Svetlana; Mikić, Aleksandar; Maksimović, Vesna

(International Legume Society, 2013)

TY  - CONF
AU  - Stanisavljević, Nemanja
AU  - Vukotić, Goran
AU  - Fira, Đorđe
AU  - Jovanović, Živko
AU  - Miljuš-Đukić, Jovanka
AU  - Radović, Svetlana
AU  - Mikić, Aleksandar
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3040
AB  - In recent years antioxidant properties of plant derived protein hydrolysates were established. Antioxidative activity of these hydrolysates largely depends on specificity of the proteinase used for hydrolysis, duration of proteinase treatment, as well as composition and molecular weight of hydrolysis derived peptides. In order to explore potential use of nine proteinase containing Lactobacillus strains in production of antioxidative peptides we tested their ability to hydrolyze lyophilized hydrosoluble protein fraction from dehulled and defatted dry pea seeds (Pisum sativum), cultivar NS Mraz. We also developed plant protein based liquid medium for cultivation of lactobacilli which was later used as starter for hydrolysis reaction in larger volume. We found that all nine strains could hydrolyze plant hydrosoluble fraction of pea seed proteins but with different efficiency. We also examined the effect of duration of hydrolysis on antioxidative activity in <10kDa fraction of obtained peptide extracts, using most proteolyticaly active strain, human vaginal isolate Lactobacillus rhamnosus T10. General conclusion was that antioxidative activity was positively correlated with increase of hydrolysis duration and amount of small peptides, which reached up to 34% of total proteins after 24h of hydrolysis treatment.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity
EP  - 286
SP  - 286
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3040
ER  - 
@conference{
author = "Stanisavljević, Nemanja and Vukotić, Goran and Fira, Đorđe and Jovanović, Živko and Miljuš-Đukić, Jovanka and Radović, Svetlana and Mikić, Aleksandar and Maksimović, Vesna",
year = "2013",
abstract = "In recent years antioxidant properties of plant derived protein hydrolysates were established. Antioxidative activity of these hydrolysates largely depends on specificity of the proteinase used for hydrolysis, duration of proteinase treatment, as well as composition and molecular weight of hydrolysis derived peptides. In order to explore potential use of nine proteinase containing Lactobacillus strains in production of antioxidative peptides we tested their ability to hydrolyze lyophilized hydrosoluble protein fraction from dehulled and defatted dry pea seeds (Pisum sativum), cultivar NS Mraz. We also developed plant protein based liquid medium for cultivation of lactobacilli which was later used as starter for hydrolysis reaction in larger volume. We found that all nine strains could hydrolyze plant hydrosoluble fraction of pea seed proteins but with different efficiency. We also examined the effect of duration of hydrolysis on antioxidative activity in <10kDa fraction of obtained peptide extracts, using most proteolyticaly active strain, human vaginal isolate Lactobacillus rhamnosus T10. General conclusion was that antioxidative activity was positively correlated with increase of hydrolysis duration and amount of small peptides, which reached up to 34% of total proteins after 24h of hydrolysis treatment.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity",
pages = "286-286",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3040"
}
Stanisavljević, N., Vukotić, G., Fira, Đ., Jovanović, Ž., Miljuš-Đukić, J., Radović, S., Mikić, A.,& Maksimović, V.. (2013). Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 286-286.
https://hdl.handle.net/21.15107/rcub_fiver_3040
Stanisavljević N, Vukotić G, Fira Đ, Jovanović Ž, Miljuš-Đukić J, Radović S, Mikić A, Maksimović V. Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:286-286.
https://hdl.handle.net/21.15107/rcub_fiver_3040 .
Stanisavljević, Nemanja, Vukotić, Goran, Fira, Đorđe, Jovanović, Živko, Miljuš-Đukić, Jovanka, Radović, Svetlana, Mikić, Aleksandar, Maksimović, Vesna, "Application of different Lactobacillus strains in production pea seed protein hydrolysates with antioxidative activity" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):286-286,
https://hdl.handle.net/21.15107/rcub_fiver_3040 .

Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes

Radović, Svetlana; Mikić, Aleksandar; Jovanović, Živko; Stanisavljević, Nemanja; Krstić, Đorđe; Ćupina, Branko

(International Legume Society, 2013)

TY  - CONF
AU  - Radović, Svetlana
AU  - Mikić, Aleksandar
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Krstić, Đorđe
AU  - Ćupina, Branko
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3038
AB  - Pea is one of the most important crops in the world. Among the many ways of utilization of this crop are that related for forage production. Forage pea may be cultivated alone as a pure crop, or in mixture with other crops, usually cereals. In recent time, several attempts have been made to cultivate forage pea with other legumes, where pea would play the role of cover crop. The results reported here are obtained in a greenhouse experiment aimed to assess the pea as a companion crop for establishment of lucerne (Medicago sativa L.), red clover (Trifolium pratense L.) and sainfoin (Onobrychis sativa L.) as undersown crops. The semi-leafless pea cultivar Jezero with three population densities (30, 60 and 90 plants per m2) were used in this experiment. Two control treatments also included in the experiment – the perennial legumes grown as a pure stand, and the perennial legumes with oat as a cover crop. The effect of pea on forage yield, chlorophyll content, leaf surface and LER is determined. According to obtained results, the best combination for lucerne is 30 pea plants per m2, 90 for red clover and 60 pea plants per m2 for sainfoin.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes
EP  - 265
SP  - 265
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3038
ER  - 
@conference{
author = "Radović, Svetlana and Mikić, Aleksandar and Jovanović, Živko and Stanisavljević, Nemanja and Krstić, Đorđe and Ćupina, Branko",
year = "2013",
abstract = "Pea is one of the most important crops in the world. Among the many ways of utilization of this crop are that related for forage production. Forage pea may be cultivated alone as a pure crop, or in mixture with other crops, usually cereals. In recent time, several attempts have been made to cultivate forage pea with other legumes, where pea would play the role of cover crop. The results reported here are obtained in a greenhouse experiment aimed to assess the pea as a companion crop for establishment of lucerne (Medicago sativa L.), red clover (Trifolium pratense L.) and sainfoin (Onobrychis sativa L.) as undersown crops. The semi-leafless pea cultivar Jezero with three population densities (30, 60 and 90 plants per m2) were used in this experiment. Two control treatments also included in the experiment – the perennial legumes grown as a pure stand, and the perennial legumes with oat as a cover crop. The effect of pea on forage yield, chlorophyll content, leaf surface and LER is determined. According to obtained results, the best combination for lucerne is 30 pea plants per m2, 90 for red clover and 60 pea plants per m2 for sainfoin.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes",
pages = "265-265",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3038"
}
Radović, S., Mikić, A., Jovanović, Ž., Stanisavljević, N., Krstić, Đ.,& Ćupina, B.. (2013). Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 265-265.
https://hdl.handle.net/21.15107/rcub_fiver_3038
Radović S, Mikić A, Jovanović Ž, Stanisavljević N, Krstić Đ, Ćupina B. Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:265-265.
https://hdl.handle.net/21.15107/rcub_fiver_3038 .
Radović, Svetlana, Mikić, Aleksandar, Jovanović, Živko, Stanisavljević, Nemanja, Krstić, Đorđe, Ćupina, Branko, "Field pea (Pisum sativum L.) as a companion crop for the establishment of perennial legumes" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):265-265,
https://hdl.handle.net/21.15107/rcub_fiver_3038 .

Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures

Miljuš-Đukić, Jovanka; Stanisavljević, Nemanja; Radović, Svetlana; Jovanović, Živko; Mikić, Aleksandar; Maksimović, Vesna

(International Legume Society, 2013)

TY  - CONF
AU  - Miljuš-Đukić, Jovanka
AU  - Stanisavljević, Nemanja
AU  - Radović, Svetlana
AU  - Jovanović, Živko
AU  - Mikić, Aleksandar
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3024
AB  - Salinity is one of the major abiotic factors limiting plants productivity. Processes, such as seed germination, seedling growth and vigor, vegetative growth, flowering, and fruit set, are adversely affected by high saltconcentrations, ultimately causing diminished economic yield and also quality of products. Shoots of micropropagated Pisum fulvum, P. sativum and P. arvense were treated with 75, 120, 150 and 200 mMNaClin order to study pea tissues capacity to tolerate salt stress and their antioxidant capacity. We used the phenol content and DPPH antioxidant assay as oxidative damage indicators. Results showed that salt stress caused slower plant growth, leading to an increase in lipid peroxidation and a decrease in total chlorophyll in pea tissues. Further, results showed that P. fulvum is to be considered as potentially less sensitive to high NaCl concentrations in comparison to P. sativum and P. arvense and could represents a gene pool which could be used for improving the characteristics of field pea.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures
EP  - 207
SP  - 207
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3024
ER  - 
@conference{
author = "Miljuš-Đukić, Jovanka and Stanisavljević, Nemanja and Radović, Svetlana and Jovanović, Živko and Mikić, Aleksandar and Maksimović, Vesna",
year = "2013",
abstract = "Salinity is one of the major abiotic factors limiting plants productivity. Processes, such as seed germination, seedling growth and vigor, vegetative growth, flowering, and fruit set, are adversely affected by high saltconcentrations, ultimately causing diminished economic yield and also quality of products. Shoots of micropropagated Pisum fulvum, P. sativum and P. arvense were treated with 75, 120, 150 and 200 mMNaClin order to study pea tissues capacity to tolerate salt stress and their antioxidant capacity. We used the phenol content and DPPH antioxidant assay as oxidative damage indicators. Results showed that salt stress caused slower plant growth, leading to an increase in lipid peroxidation and a decrease in total chlorophyll in pea tissues. Further, results showed that P. fulvum is to be considered as potentially less sensitive to high NaCl concentrations in comparison to P. sativum and P. arvense and could represents a gene pool which could be used for improving the characteristics of field pea.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures",
pages = "207-207",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3024"
}
Miljuš-Đukić, J., Stanisavljević, N., Radović, S., Jovanović, Ž., Mikić, A.,& Maksimović, V.. (2013). Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 207-207.
https://hdl.handle.net/21.15107/rcub_fiver_3024
Miljuš-Đukić J, Stanisavljević N, Radović S, Jovanović Ž, Mikić A, Maksimović V. Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:207-207.
https://hdl.handle.net/21.15107/rcub_fiver_3024 .
Miljuš-Đukić, Jovanka, Stanisavljević, Nemanja, Radović, Svetlana, Jovanović, Živko, Mikić, Aleksandar, Maksimović, Vesna, "Salt stress response in three pea species (Pisum arvense, P. sativum and P. fulvum) in vitro cultures" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):207-207,
https://hdl.handle.net/21.15107/rcub_fiver_3024 .

Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.)

Jovanović, Živko; Stanisavljević, Nemanja; Miljuš-Đukić, Jovanka; Mikić, Aleksandar; Radović, Svetlana; Ćupina, Branko; Maksimović, Vesna

(International Legume Society, 2013)

TY  - CONF
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Miljuš-Đukić, Jovanka
AU  - Mikić, Aleksandar
AU  - Radović, Svetlana
AU  - Ćupina, Branko
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3018
AB  - MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at posttranscriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that that could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame could reflect the changes as closely as possible those during water stress cause visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. On the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) was not changed in roots and shoots of water-stressed plants, compared to control-well hydrated plants. This suggests that COX5b is not target of miR398, or that its expression is regulated by some other mechanism. P1B-ATPase expression increased during water deficit only in shoots of pea; in the roots there was no changes in expression. Our results might help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.)
EP  - 153
SP  - 153
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3018
ER  - 
@conference{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Miljuš-Đukić, Jovanka and Mikić, Aleksandar and Radović, Svetlana and Ćupina, Branko and Maksimović, Vesna",
year = "2013",
abstract = "MicroRNAs (miRNAs), recently recognized as important regulator of gene expression at posttranscriptional level, have been found to be involved in plant stress responses. The observation that some miRNAs are up- or down regulated by stress implies that that could play vital roles in plant resistance to abiotic and biotic stress. We investigated the effect of water stress treatment during 10 days on expression of conserved miRNAs-miR398a/b and miR408 in pea plants. This time frame could reflect the changes as closely as possible those during water stress cause visible effects under field condition. It was observed that dehydration strongly down regulates the expression of both miR398a/b and miR408 in pea roots and shoots. The down-regulation of miR398a/b and the up-regulation of potential target genes copper superoxide dismutase, CSD1, highlight the involvement of this miRNA in pea stress response. On the contrary, the mRNA level of cytochrome c oxidase subunit 5 (COX5b) was not changed in roots and shoots of water-stressed plants, compared to control-well hydrated plants. This suggests that COX5b is not target of miR398, or that its expression is regulated by some other mechanism. P1B-ATPase expression increased during water deficit only in shoots of pea; in the roots there was no changes in expression. Our results might help to understand the possible role of investigated miRNAs and their contribution to pea capacity to cope with water deficit.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.)",
pages = "153-153",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3018"
}
Jovanović, Ž., Stanisavljević, N., Miljuš-Đukić, J., Mikić, A., Radović, S., Ćupina, B.,& Maksimović, V.. (2013). Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 153-153.
https://hdl.handle.net/21.15107/rcub_fiver_3018
Jovanović Ž, Stanisavljević N, Miljuš-Đukić J, Mikić A, Radović S, Ćupina B, Maksimović V. Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.). in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:153-153.
https://hdl.handle.net/21.15107/rcub_fiver_3018 .
Jovanović, Živko, Stanisavljević, Nemanja, Miljuš-Đukić, Jovanka, Mikić, Aleksandar, Radović, Svetlana, Ćupina, Branko, Maksimović, Vesna, "Dehydration affected the expression of miR398 and miR408 in pea (Pisum sativum L.)" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):153-153,
https://hdl.handle.net/21.15107/rcub_fiver_3018 .

Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia

Stanisavljević, Nemanja; Jovanović, Živko; Čupić, Tihomir; Lukić, Jovanka; Miljuš-Đukić, Jovanka; Radović, Svetlana; Mikić, Aleksandar; Maksimović, Vesna

(International Legume Society, 2013)

TY  - CONF
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Čupić, Tihomir
AU  - Lukić, Jovanka
AU  - Miljuš-Đukić, Jovanka
AU  - Radović, Svetlana
AU  - Mikić, Aleksandar
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3007
AB  - Field pea (Pisum sativum var. arvense) and grass pea (Lathyrus sativus) are important high protein crops used as feed. Legume seeds are also rich in many other nutrient compounds including starch, sugar, dietary fiber and micronutrients (vitamins and minerals.) They are also a rich source of many bioactive non-nutrient compounds including phenolic antioxidants. Besides their beneficial effects, some phenolic compounds, especially condensed tannins in feed, are considered as antinutritional factors for ruminants, owing to their capacity for binding to macromolecules such as fiber and proteins. Seed mass and diameter are valuable parameters used in breeding, as well as seed mineral composition, since significant quantitative trait loci were identified for Ca, Fe, Mn, K, Mg, Ni, P and Zn. Thus we conducted this study in order to determine some parameters, in three cultivars of previously non-tested field pea and five grass pea cultivars from Croatia, which are important for breeders. We determined seed mass, diameter and proximate mineral composition as well as content of soluble sugars and total phenolic content in dry seeds of these cultivars. Cultivars differed significantly in total phenollics and mineral composition.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia
EP  - 104
SP  - 104
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3007
ER  - 
@conference{
author = "Stanisavljević, Nemanja and Jovanović, Živko and Čupić, Tihomir and Lukić, Jovanka and Miljuš-Đukić, Jovanka and Radović, Svetlana and Mikić, Aleksandar and Maksimović, Vesna",
year = "2013",
abstract = "Field pea (Pisum sativum var. arvense) and grass pea (Lathyrus sativus) are important high protein crops used as feed. Legume seeds are also rich in many other nutrient compounds including starch, sugar, dietary fiber and micronutrients (vitamins and minerals.) They are also a rich source of many bioactive non-nutrient compounds including phenolic antioxidants. Besides their beneficial effects, some phenolic compounds, especially condensed tannins in feed, are considered as antinutritional factors for ruminants, owing to their capacity for binding to macromolecules such as fiber and proteins. Seed mass and diameter are valuable parameters used in breeding, as well as seed mineral composition, since significant quantitative trait loci were identified for Ca, Fe, Mn, K, Mg, Ni, P and Zn. Thus we conducted this study in order to determine some parameters, in three cultivars of previously non-tested field pea and five grass pea cultivars from Croatia, which are important for breeders. We determined seed mass, diameter and proximate mineral composition as well as content of soluble sugars and total phenolic content in dry seeds of these cultivars. Cultivars differed significantly in total phenollics and mineral composition.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia",
pages = "104-104",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3007"
}
Stanisavljević, N., Jovanović, Ž., Čupić, T., Lukić, J., Miljuš-Đukić, J., Radović, S., Mikić, A.,& Maksimović, V.. (2013). Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 104-104.
https://hdl.handle.net/21.15107/rcub_fiver_3007
Stanisavljević N, Jovanović Ž, Čupić T, Lukić J, Miljuš-Đukić J, Radović S, Mikić A, Maksimović V. Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:104-104.
https://hdl.handle.net/21.15107/rcub_fiver_3007 .
Stanisavljević, Nemanja, Jovanović, Živko, Čupić, Tihomir, Lukić, Jovanka, Miljuš-Đukić, Jovanka, Radović, Svetlana, Mikić, Aleksandar, Maksimović, Vesna, "Analysis of some nutritional and antinutritional compounds in dry seeds of grass pea and field pea cultivars from Croatia" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):104-104,
https://hdl.handle.net/21.15107/rcub_fiver_3007 .

Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts

Stanisavljević, Nemanja; Jovanović, Živko; Čupić, Tihomir; Lukić, Jovanka; Miljuš-Đukić, Jovanka; Radović, Svetlana; Mikić, Aleksandar; Maksimović, Vesna

(International Legume Society, 2013)

TY  - CONF
AU  - Stanisavljević, Nemanja
AU  - Jovanović, Živko
AU  - Čupić, Tihomir
AU  - Lukić, Jovanka
AU  - Miljuš-Đukić, Jovanka
AU  - Radović, Svetlana
AU  - Mikić, Aleksandar
AU  - Maksimović, Vesna
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2998
AB  - Antioxidant activities and phenolic contents were studied in methanolic and water extracts of non-processed, cooked and in vitro enzymatically digested seed flour. Antioxidant activities of total protein hydrolysates and small peptide fractions (MW<3kDa and MW<3kDa) isolated from seed were also determined. Study showed that cooking and enzymatic digestion strongly enhanced release of phenolic compounds. Scavenging activity against 2,2-diphenyl-1-picryhydrazyl radical (DPPH•), hydroxyl radical and hydrogen peroxide were also increased. Protein hydrolysates especially MW<3kDa fraction possess significant antioxidative potential. Taken together our findings indicate that in methanolic and water extracts of cooked and digested seed flour small peptide fraction, beside phenolic compounds, can also significantly contribute to free radical and hydrogen peroxide scavenging activity.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts
EP  - 63
SP  - 63
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2998
ER  - 
@conference{
author = "Stanisavljević, Nemanja and Jovanović, Živko and Čupić, Tihomir and Lukić, Jovanka and Miljuš-Đukić, Jovanka and Radović, Svetlana and Mikić, Aleksandar and Maksimović, Vesna",
year = "2013",
abstract = "Antioxidant activities and phenolic contents were studied in methanolic and water extracts of non-processed, cooked and in vitro enzymatically digested seed flour. Antioxidant activities of total protein hydrolysates and small peptide fractions (MW<3kDa and MW<3kDa) isolated from seed were also determined. Study showed that cooking and enzymatic digestion strongly enhanced release of phenolic compounds. Scavenging activity against 2,2-diphenyl-1-picryhydrazyl radical (DPPH•), hydroxyl radical and hydrogen peroxide were also increased. Protein hydrolysates especially MW<3kDa fraction possess significant antioxidative potential. Taken together our findings indicate that in methanolic and water extracts of cooked and digested seed flour small peptide fraction, beside phenolic compounds, can also significantly contribute to free radical and hydrogen peroxide scavenging activity.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts",
pages = "63-63",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2998"
}
Stanisavljević, N., Jovanović, Ž., Čupić, T., Lukić, J., Miljuš-Đukić, J., Radović, S., Mikić, A.,& Maksimović, V.. (2013). Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 63-63.
https://hdl.handle.net/21.15107/rcub_fiver_2998
Stanisavljević N, Jovanović Ž, Čupić T, Lukić J, Miljuš-Đukić J, Radović S, Mikić A, Maksimović V. Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:63-63.
https://hdl.handle.net/21.15107/rcub_fiver_2998 .
Stanisavljević, Nemanja, Jovanović, Živko, Čupić, Tihomir, Lukić, Jovanka, Miljuš-Đukić, Jovanka, Radović, Svetlana, Mikić, Aleksandar, Maksimović, Vesna, "Simple cooking and in vitro gastrointestinal digestion improves antioxidant activity of legume seed flour extracts" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):63-63,
https://hdl.handle.net/21.15107/rcub_fiver_2998 .

A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia

Medović, Aleksandar; Smykal, Petr; Jovanović, Živko; Stanisavljević, Nemanja; Zlatković, Bojan; Ćupina, Branko; Đorđević, Vuk; Mikić, Aleksandar

(International Legume Society, 2013)

TY  - CONF
AU  - Medović, Aleksandar
AU  - Smykal, Petr
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Zlatković, Bojan
AU  - Ćupina, Branko
AU  - Đorđević, Vuk
AU  - Mikić, Aleksandar
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/2983
AB  - A unique example of 2,572 carbonized pea seeds was recovered from the 11th century B.C. deposits at Hissar, a multilevel settlement of the Brnjica cultural group (1,350-1,000 B.C.) in southeast Serbia. Five hundred seeds maintained pea-like hilum, only few had preserved intact smooth-surfaced testa and the majority of seeds had no seed coat. Fortunately, only few seeds of other pulses were observed in the sample and their identity was clearly determined. Applying the exclusion principle, all the “naked”, 3-4 mm large seeds, mainly broad ellipsoid and less often globose, with flattenings or concavities, were determined to correspond to cultivated pea (Pisum sativum L.). Additionally, a high thousand seed weight of charred seeds (24.4 g) suggested cultivated status. To confirm our finding, we processed two samples with molecular tools. A sequence analysis of four chloroplast DNA loci (trnSG, trnK, matK and rbcL) in total length of 1329bp, showed intermediate position to cultivated P. sativum and wild P. sativum subsp. elatius.
PB  - International Legume Society
PB  - Novi Sad : Institute of Field and Vegetable Crops
C3  - Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
T1  - A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia
EP  - 28
SP  - 28
UR  - https://hdl.handle.net/21.15107/rcub_fiver_2983
ER  - 
@conference{
author = "Medović, Aleksandar and Smykal, Petr and Jovanović, Živko and Stanisavljević, Nemanja and Zlatković, Bojan and Ćupina, Branko and Đorđević, Vuk and Mikić, Aleksandar",
year = "2013",
abstract = "A unique example of 2,572 carbonized pea seeds was recovered from the 11th century B.C. deposits at Hissar, a multilevel settlement of the Brnjica cultural group (1,350-1,000 B.C.) in southeast Serbia. Five hundred seeds maintained pea-like hilum, only few had preserved intact smooth-surfaced testa and the majority of seeds had no seed coat. Fortunately, only few seeds of other pulses were observed in the sample and their identity was clearly determined. Applying the exclusion principle, all the “naked”, 3-4 mm large seeds, mainly broad ellipsoid and less often globose, with flattenings or concavities, were determined to correspond to cultivated pea (Pisum sativum L.). Additionally, a high thousand seed weight of charred seeds (24.4 g) suggested cultivated status. To confirm our finding, we processed two samples with molecular tools. A sequence analysis of four chloroplast DNA loci (trnSG, trnK, matK and rbcL) in total length of 1329bp, showed intermediate position to cultivated P. sativum and wild P. sativum subsp. elatius.",
publisher = "International Legume Society, Novi Sad : Institute of Field and Vegetable Crops",
journal = "Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad",
title = "A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia",
pages = "28-28",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2983"
}
Medović, A., Smykal, P., Jovanović, Ž., Stanisavljević, N., Zlatković, B., Ćupina, B., Đorđević, V.,& Mikić, A.. (2013). A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad
International Legume Society., 28-28.
https://hdl.handle.net/21.15107/rcub_fiver_2983
Medović A, Smykal P, Jovanović Ž, Stanisavljević N, Zlatković B, Ćupina B, Đorđević V, Mikić A. A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia. in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad. 2013;:28-28.
https://hdl.handle.net/21.15107/rcub_fiver_2983 .
Medović, Aleksandar, Smykal, Petr, Jovanović, Živko, Stanisavljević, Nemanja, Zlatković, Bojan, Ćupina, Branko, Đorđević, Vuk, Mikić, Aleksandar, "A combined archaeobotanical and palaeogenetic analysis of charred pea (Pisum sativum) seeds from an Early Iron Age storage pit at the hill fort settlement Hissar, Leskovac, southeast Serbia" in Book of Abstracts, 1st Legume Society Conference 2013: A Legume Odyssey, 9-11 May 2013, Novi Sad (2013):28-28,
https://hdl.handle.net/21.15107/rcub_fiver_2983 .

Developing fall-sown pea cultivars as an answer to the challenges of climatic changes

Mikić, Aleksandar; Mihailović, Vojislav; Ćupina, Branko; Lejeune-Henaut, Isabelle; Hanocq, E.; Duc, Gerard; McPhee, Kevin; Stoddard, Frederick L.; Kosev, Valentin; Krstić, Đorđe; Antanasović, Svetlana; Jovanović, Živko

(Nova Science Publishers, Inc., 2012)

TY  - CHAP
AU  - Mikić, Aleksandar
AU  - Mihailović, Vojislav
AU  - Ćupina, Branko
AU  - Lejeune-Henaut, Isabelle
AU  - Hanocq, E.
AU  - Duc, Gerard
AU  - McPhee, Kevin
AU  - Stoddard, Frederick L.
AU  - Kosev, Valentin
AU  - Krstić, Đorđe
AU  - Antanasović, Svetlana
AU  - Jovanović, Živko
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/1166
AB  - Pea is considered rather well adapted to wide temperature ranges, with seedlings able to survive even -20 °C. From a physiological viewpoint, pea becomes tolerant to frost if first exposed to low non-freezing temperatures, causing the so-called cold acclimation. Delayed floral initiation helps some forage pea genotypes to escape the main winter freezing periods, as susceptibility to frost increases during the transition to the reproductive state. The oldest winter pea cultivars carry the dominant allele, Hr, although some bear hr. They are generally characterized by prominent winter hardiness and a long growing season, from sowing in early October until either cutting for forage production in late May or harvesting seeds in mid-July. The average forage yields in the winter forage pea cultivars often exceed 45 t ha-1 of green forage, 9 t ha-1 of forage dry matter and 1700 kg ha-1 of forage crude protein. Modern dry pea cultivars have advanced winter hardiness and enhanced dry grain production. They are already in use in other temperate regions in both Europe, especially France, and the USA. One of the strategic advantages of fall-sown dry pea cultivars of recent release is their significantly improved earliness. These cultivars are regularly at least one week earlier than winter barley, providing many farmers with the novel opportunity of not having to choose between pea and cereals, since many have only one combine harvester available and give priority to their cereals. Furthermore, fall-sown dry pea cultivars may have increased grain dry matter crude protein content and it is possible to merge winter hardiness and low content of anti-nutritional factors. Low thousand seed weight, not exceeding 200 g, and a population density of 75-80 plants m-2 provide inexpensive sowing. All these outcomes should result in an increased area and production of dry pea in many temperate regions. In the end, growing winter-hardy pea cultivars also mean a significant shift into the wetter half of the year and thus mitigating more and more prominent and unpredictable effects of spring droughts, demonstrating an efficient answer to the challenges of climatic changes.
PB  - Nova Science Publishers, Inc.
T2  - Peas: Cultivation, Varieties & Nutritional Uses
T1  - Developing fall-sown pea cultivars as an answer to the challenges of climatic changes
EP  - 124
SP  - 107
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1166
ER  - 
@inbook{
author = "Mikić, Aleksandar and Mihailović, Vojislav and Ćupina, Branko and Lejeune-Henaut, Isabelle and Hanocq, E. and Duc, Gerard and McPhee, Kevin and Stoddard, Frederick L. and Kosev, Valentin and Krstić, Đorđe and Antanasović, Svetlana and Jovanović, Živko",
year = "2012",
abstract = "Pea is considered rather well adapted to wide temperature ranges, with seedlings able to survive even -20 °C. From a physiological viewpoint, pea becomes tolerant to frost if first exposed to low non-freezing temperatures, causing the so-called cold acclimation. Delayed floral initiation helps some forage pea genotypes to escape the main winter freezing periods, as susceptibility to frost increases during the transition to the reproductive state. The oldest winter pea cultivars carry the dominant allele, Hr, although some bear hr. They are generally characterized by prominent winter hardiness and a long growing season, from sowing in early October until either cutting for forage production in late May or harvesting seeds in mid-July. The average forage yields in the winter forage pea cultivars often exceed 45 t ha-1 of green forage, 9 t ha-1 of forage dry matter and 1700 kg ha-1 of forage crude protein. Modern dry pea cultivars have advanced winter hardiness and enhanced dry grain production. They are already in use in other temperate regions in both Europe, especially France, and the USA. One of the strategic advantages of fall-sown dry pea cultivars of recent release is their significantly improved earliness. These cultivars are regularly at least one week earlier than winter barley, providing many farmers with the novel opportunity of not having to choose between pea and cereals, since many have only one combine harvester available and give priority to their cereals. Furthermore, fall-sown dry pea cultivars may have increased grain dry matter crude protein content and it is possible to merge winter hardiness and low content of anti-nutritional factors. Low thousand seed weight, not exceeding 200 g, and a population density of 75-80 plants m-2 provide inexpensive sowing. All these outcomes should result in an increased area and production of dry pea in many temperate regions. In the end, growing winter-hardy pea cultivars also mean a significant shift into the wetter half of the year and thus mitigating more and more prominent and unpredictable effects of spring droughts, demonstrating an efficient answer to the challenges of climatic changes.",
publisher = "Nova Science Publishers, Inc.",
journal = "Peas: Cultivation, Varieties & Nutritional Uses",
booktitle = "Developing fall-sown pea cultivars as an answer to the challenges of climatic changes",
pages = "124-107",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1166"
}
Mikić, A., Mihailović, V., Ćupina, B., Lejeune-Henaut, I., Hanocq, E., Duc, G., McPhee, K., Stoddard, F. L., Kosev, V., Krstić, Đ., Antanasović, S.,& Jovanović, Ž.. (2012). Developing fall-sown pea cultivars as an answer to the challenges of climatic changes. in Peas: Cultivation, Varieties & Nutritional Uses
Nova Science Publishers, Inc.., 107-124.
https://hdl.handle.net/21.15107/rcub_fiver_1166
Mikić A, Mihailović V, Ćupina B, Lejeune-Henaut I, Hanocq E, Duc G, McPhee K, Stoddard FL, Kosev V, Krstić Đ, Antanasović S, Jovanović Ž. Developing fall-sown pea cultivars as an answer to the challenges of climatic changes. in Peas: Cultivation, Varieties & Nutritional Uses. 2012;:107-124.
https://hdl.handle.net/21.15107/rcub_fiver_1166 .
Mikić, Aleksandar, Mihailović, Vojislav, Ćupina, Branko, Lejeune-Henaut, Isabelle, Hanocq, E., Duc, Gerard, McPhee, Kevin, Stoddard, Frederick L., Kosev, Valentin, Krstić, Đorđe, Antanasović, Svetlana, Jovanović, Živko, "Developing fall-sown pea cultivars as an answer to the challenges of climatic changes" in Peas: Cultivation, Varieties & Nutritional Uses (2012):107-124,
https://hdl.handle.net/21.15107/rcub_fiver_1166 .
2

Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia

Jovanović, Živko; Stanisavljević, Nemanja; Nikolić, Aleksandra; Medović, Aleksandar; Mikić, Aleksandar; Radović, Svetlana; Đorđević, Vuk

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2011)

TY  - JOUR
AU  - Jovanović, Živko
AU  - Stanisavljević, Nemanja
AU  - Nikolić, Aleksandra
AU  - Medović, Aleksandar
AU  - Mikić, Aleksandar
AU  - Radović, Svetlana
AU  - Đorđević, Vuk
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/934
AB  - The extracts were prepared from the samples of 3,200-year-old charred pea and bitter vetch seeds from the site of Hissar near Leskovac, South Serbia, using two different DNA extraction procedures. We used CTAB method with some modification and obtained low quantity of ancient DNA in comparison with the second method used - commercial available kit. After the extraction, a whole genome amplification using Phi29 DNA polymerase was performed. The amplified DNAs were used for PCR reaction using primers for 26S rDNA gene, which is located on the nuclear genome. The single band corresponding to 26S rDNA fragment from modern relatives was obtained. We conclude that DNA from charred pea and vetch seed can be extracted and used for further archaeobotanical analysis at the molecular level.
AB  - Primenom dve različite procedure ekstrahovana je drevna DNK iz ugljenisanih semena graška i urova starih 3200 godina sa lokaliteta Hisar kod Leskovca. Korišćena je modifikovana CTAB metoda i dobijena je relativno mala količina drevne DNK u poređenju sa drugom primenjenom metodom - komercijalno dostupnim kitom za izolaciju DNK. Nakon ekstrakcije primenjeno je umnožavanje celog genoma pomoću Phi29 DNK polimeraze. Umnožena DNK je korišćena za PCR reakciju primenom prajmera za 26S rDNK gen, koji je lociran u jedarnom genomu. Dobijen je fragment iste veličine kao i PCR fragment 26S rDNK savremenih srodnika graška i urova. Može se zaključiti da je moguće ekstrahovati drevnu DNK iz ugljenisanih semena graška i urova i koristiti je za dalje arheobotaničke analize na molekularnom nivou.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia
T1  - Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji
EP  - 232
IS  - 1
SP  - 227
VL  - 48
DO  - 10.5937/ratpov1101227J
ER  - 
@article{
author = "Jovanović, Živko and Stanisavljević, Nemanja and Nikolić, Aleksandra and Medović, Aleksandar and Mikić, Aleksandar and Radović, Svetlana and Đorđević, Vuk",
year = "2011",
abstract = "The extracts were prepared from the samples of 3,200-year-old charred pea and bitter vetch seeds from the site of Hissar near Leskovac, South Serbia, using two different DNA extraction procedures. We used CTAB method with some modification and obtained low quantity of ancient DNA in comparison with the second method used - commercial available kit. After the extraction, a whole genome amplification using Phi29 DNA polymerase was performed. The amplified DNAs were used for PCR reaction using primers for 26S rDNA gene, which is located on the nuclear genome. The single band corresponding to 26S rDNA fragment from modern relatives was obtained. We conclude that DNA from charred pea and vetch seed can be extracted and used for further archaeobotanical analysis at the molecular level., Primenom dve različite procedure ekstrahovana je drevna DNK iz ugljenisanih semena graška i urova starih 3200 godina sa lokaliteta Hisar kod Leskovca. Korišćena je modifikovana CTAB metoda i dobijena je relativno mala količina drevne DNK u poređenju sa drugom primenjenom metodom - komercijalno dostupnim kitom za izolaciju DNK. Nakon ekstrakcije primenjeno je umnožavanje celog genoma pomoću Phi29 DNK polimeraze. Umnožena DNK je korišćena za PCR reakciju primenom prajmera za 26S rDNK gen, koji je lociran u jedarnom genomu. Dobijen je fragment iste veličine kao i PCR fragment 26S rDNK savremenih srodnika graška i urova. Može se zaključiti da je moguće ekstrahovati drevnu DNK iz ugljenisanih semena graška i urova i koristiti je za dalje arheobotaničke analize na molekularnom nivou.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia, Grašak i urov Tetovac - made in ranogvozdenodobni Leskovac, Deo drugi - ekstrakcija drevne DNK iz ugljenisanih semena sa nalazišta Hisar u južnoj Srbiji",
pages = "232-227",
number = "1",
volume = "48",
doi = "10.5937/ratpov1101227J"
}
Jovanović, Ž., Stanisavljević, N., Nikolić, A., Medović, A., Mikić, A., Radović, S.,& Đorđević, V.. (2011). Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 48(1), 227-232.
https://doi.org/10.5937/ratpov1101227J
Jovanović Ž, Stanisavljević N, Nikolić A, Medović A, Mikić A, Radović S, Đorđević V. Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2011;48(1):227-232.
doi:10.5937/ratpov1101227J .
Jovanović, Živko, Stanisavljević, Nemanja, Nikolić, Aleksandra, Medović, Aleksandar, Mikić, Aleksandar, Radović, Svetlana, Đorđević, Vuk, "Pisum & Ervilia Tetovac: Made in Early Iron Age Leskovac, Part two: Extraction of the ancient DNA from charred seeds from the site of Hissar in South Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 48, no. 1 (2011):227-232,
https://doi.org/10.5937/ratpov1101227J . .
14