Ćalić, Irena

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orcid::0000-0002-5483-4516
  • Ćalić, Irena (5)
  • Radinović, Irena (2)
Projects

Author's Bibliography

Variability of red clover genotypes on the basis of morphological markers

Radinović, Irena; Vasiljević, Sanja; Zorić, Miroslav; Branković, Gordana; Živanović, Tomislav; Prodanović, Slaven

(Društvo genetičara Srbije, Beograd, 2018)

TY  - JOUR
AU  - Radinović, Irena
AU  - Vasiljević, Sanja
AU  - Zorić, Miroslav
AU  - Branković, Gordana
AU  - Živanović, Tomislav
AU  - Prodanović, Slaven
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1753
AB  - Red clover (Trifolium pratense L.) is an important forage legume of temperate regions dominantly used as a source of animal food. The present research aimed at assessment of genetic diversity based on morphological markers, through the analyses of five morphological markers in a collection of 46 red clover genotypes. These morphological markers were screened according to the UPOV descriptor (2001) in the trial laid out in a randomized complete block design with three replications. The traits analyzed and investigated were: time of flowering, growth habit, density of hairs, leaf color and intensity of white marks. The average value of Shannon's diversity index for five morphological markers amounted 0.711. Homogeneity analysis (HOMALS) of the same five descriptors accounted for 71.2% of the total variation of the standardized data, with the first and second axis explaining 38.4% and 32.8% of the morphological variability, respectively. Based on this analyses all red clover genotypes were grouped into seven homogeneous groups in two-dimensional space, thus providing visualization of genotypes diversity based on their morphological traits. UPGMA cluster analysis of the same morphological markers allowed the description of four groups with genetic distances represented by the simple matching coefficient of similarity ranging from 0.00 to 1.00. Observed results achieved by these two analyses were compared and although both of analyses were successful in grouping and discrimination of red clover genotypes with some similarities and differences, priority in future breeding programs was given to the HOMALS.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Variability of red clover genotypes on the basis of morphological markers
EP  - 906
IS  - 3
SP  - 895
VL  - 50
DO  - 10.2298/GENSR1803895R
ER  - 
@article{
author = "Radinović, Irena and Vasiljević, Sanja and Zorić, Miroslav and Branković, Gordana and Živanović, Tomislav and Prodanović, Slaven",
year = "2018",
abstract = "Red clover (Trifolium pratense L.) is an important forage legume of temperate regions dominantly used as a source of animal food. The present research aimed at assessment of genetic diversity based on morphological markers, through the analyses of five morphological markers in a collection of 46 red clover genotypes. These morphological markers were screened according to the UPOV descriptor (2001) in the trial laid out in a randomized complete block design with three replications. The traits analyzed and investigated were: time of flowering, growth habit, density of hairs, leaf color and intensity of white marks. The average value of Shannon's diversity index for five morphological markers amounted 0.711. Homogeneity analysis (HOMALS) of the same five descriptors accounted for 71.2% of the total variation of the standardized data, with the first and second axis explaining 38.4% and 32.8% of the morphological variability, respectively. Based on this analyses all red clover genotypes were grouped into seven homogeneous groups in two-dimensional space, thus providing visualization of genotypes diversity based on their morphological traits. UPGMA cluster analysis of the same morphological markers allowed the description of four groups with genetic distances represented by the simple matching coefficient of similarity ranging from 0.00 to 1.00. Observed results achieved by these two analyses were compared and although both of analyses were successful in grouping and discrimination of red clover genotypes with some similarities and differences, priority in future breeding programs was given to the HOMALS.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Variability of red clover genotypes on the basis of morphological markers",
pages = "906-895",
number = "3",
volume = "50",
doi = "10.2298/GENSR1803895R"
}
Radinović, I., Vasiljević, S., Zorić, M., Branković, G., Živanović, T.,& Prodanović, S.. (2018). Variability of red clover genotypes on the basis of morphological markers. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 50(3), 895-906.
https://doi.org/10.2298/GENSR1803895R
Radinović I, Vasiljević S, Zorić M, Branković G, Živanović T, Prodanović S. Variability of red clover genotypes on the basis of morphological markers. in Genetika-Belgrade. 2018;50(3):895-906.
doi:10.2298/GENSR1803895R .
Radinović, Irena, Vasiljević, Sanja, Zorić, Miroslav, Branković, Gordana, Živanović, Tomislav, Prodanović, Slaven, "Variability of red clover genotypes on the basis of morphological markers" in Genetika-Belgrade, 50, no. 3 (2018):895-906,
https://doi.org/10.2298/GENSR1803895R . .
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6

Molecular characterization of red clover genotypes utilizing microsatellite markers

Radinović, Irena; Vasiljević, Sanja; Branković, Gordana; Ahsyee, Salem R.; Momirović, Una; Perović, Dragan; Šurlan-Momirović, Gordana

(Inst Investigaciones Agropecuarias, Chillan, 2017)

TY  - JOUR
AU  - Radinović, Irena
AU  - Vasiljević, Sanja
AU  - Branković, Gordana
AU  - Ahsyee, Salem R.
AU  - Momirović, Una
AU  - Perović, Dragan
AU  - Šurlan-Momirović, Gordana
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1680
AB  - Genetic resources of red clover (Trifolium pratense L.) are the basis for the improvement of this important forage legume. The objective of this study was microsatellite characterization of the accessions from the collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. Molecular evaluation of 46 red clover genotypes was performed by applying the set of 14 primer pairs of microsatellite markers. These primer pairs amplified a total of 187 alleles, with an average of 13.36 alleles per locus and average polymorphism information content (PIC) value was 0.306. The minimum values of Dice genetic distances based on polymorphism of microsatellite markers were found among genotypes NCPGRU2 and NCPGRU5 (0.311) and the highest values of genetic distances were determined for a couple of genotypes Violeta and BGR2 (0.933). The average genetic distance between all pairs of genotypes amounted 0.587. The results of the principal coordinate analysis (PCoA) were consistent with the results obtained on the basis of cluster analysis, except that the PCoA allocated another four genotypes. There was no relationship between groups of genotypes formed by the use of cluster analyses and PCoA with their geographical origin. Analysis of molecular variance of 46 red clover genotypes by the status and ploidy level was significant, but it also suggested a weak genetic differentiation of groups formed on the basis of those characteristics. Observed groups of genotypes, according to the cluster analyses and PCoA of microsatellite data, could be used in future breeding programs for the selection of germplasm.
PB  - Inst Investigaciones Agropecuarias, Chillan
T2  - Chilean Journal of Agricultural Research
T1  - Molecular characterization of red clover genotypes utilizing microsatellite markers
EP  - 47
IS  - 1
SP  - 41
VL  - 77
DO  - 10.4067/S0718-58392017000100005
ER  - 
@article{
author = "Radinović, Irena and Vasiljević, Sanja and Branković, Gordana and Ahsyee, Salem R. and Momirović, Una and Perović, Dragan and Šurlan-Momirović, Gordana",
year = "2017",
abstract = "Genetic resources of red clover (Trifolium pratense L.) are the basis for the improvement of this important forage legume. The objective of this study was microsatellite characterization of the accessions from the collection of the Institute of Field and Vegetable Crops in Novi Sad, Serbia. Molecular evaluation of 46 red clover genotypes was performed by applying the set of 14 primer pairs of microsatellite markers. These primer pairs amplified a total of 187 alleles, with an average of 13.36 alleles per locus and average polymorphism information content (PIC) value was 0.306. The minimum values of Dice genetic distances based on polymorphism of microsatellite markers were found among genotypes NCPGRU2 and NCPGRU5 (0.311) and the highest values of genetic distances were determined for a couple of genotypes Violeta and BGR2 (0.933). The average genetic distance between all pairs of genotypes amounted 0.587. The results of the principal coordinate analysis (PCoA) were consistent with the results obtained on the basis of cluster analysis, except that the PCoA allocated another four genotypes. There was no relationship between groups of genotypes formed by the use of cluster analyses and PCoA with their geographical origin. Analysis of molecular variance of 46 red clover genotypes by the status and ploidy level was significant, but it also suggested a weak genetic differentiation of groups formed on the basis of those characteristics. Observed groups of genotypes, according to the cluster analyses and PCoA of microsatellite data, could be used in future breeding programs for the selection of germplasm.",
publisher = "Inst Investigaciones Agropecuarias, Chillan",
journal = "Chilean Journal of Agricultural Research",
title = "Molecular characterization of red clover genotypes utilizing microsatellite markers",
pages = "47-41",
number = "1",
volume = "77",
doi = "10.4067/S0718-58392017000100005"
}
Radinović, I., Vasiljević, S., Branković, G., Ahsyee, S. R., Momirović, U., Perović, D.,& Šurlan-Momirović, G.. (2017). Molecular characterization of red clover genotypes utilizing microsatellite markers. in Chilean Journal of Agricultural Research
Inst Investigaciones Agropecuarias, Chillan., 77(1), 41-47.
https://doi.org/10.4067/S0718-58392017000100005
Radinović I, Vasiljević S, Branković G, Ahsyee SR, Momirović U, Perović D, Šurlan-Momirović G. Molecular characterization of red clover genotypes utilizing microsatellite markers. in Chilean Journal of Agricultural Research. 2017;77(1):41-47.
doi:10.4067/S0718-58392017000100005 .
Radinović, Irena, Vasiljević, Sanja, Branković, Gordana, Ahsyee, Salem R., Momirović, Una, Perović, Dragan, Šurlan-Momirović, Gordana, "Molecular characterization of red clover genotypes utilizing microsatellite markers" in Chilean Journal of Agricultural Research, 77, no. 1 (2017):41-47,
https://doi.org/10.4067/S0718-58392017000100005 . .
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10

Genetic diversity in red clover (trifolium pratense l.) Using SSR markers

Ahsyee, Salem R.; Vasiljević, Sanja; Ćalić, Irena; Zorić, Miroslav; Karagić, Đura; Šurlan-Momirović, Gordana

(Društvo genetičara Srbije, Beograd, 2014)

TY  - JOUR
AU  - Ahsyee, Salem R.
AU  - Vasiljević, Sanja
AU  - Ćalić, Irena
AU  - Zorić, Miroslav
AU  - Karagić, Đura
AU  - Šurlan-Momirović, Gordana
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1378
AB  - Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Genetic diversity in red clover (trifolium pratense l.) Using SSR markers
EP  - 961
IS  - 3
SP  - 949
VL  - 46
DO  - 10.2298/GENSR1403949A
ER  - 
@article{
author = "Ahsyee, Salem R. and Vasiljević, Sanja and Ćalić, Irena and Zorić, Miroslav and Karagić, Đura and Šurlan-Momirović, Gordana",
year = "2014",
abstract = "Red clover (Trifolium pratense L.) is one of the main forage species from temperate regions and it's originated in Eurasia where wild-type populations are found in the Caucasus Mountains. Considerable variation for this crop has been recorded for few molecular variations. The identification and understanding of molecular genetic diversity in red clover accessions will help in effective genetic conservation along with efficient breeding programs in this crop. This study has shown molecular diversity using microsatellite markers in red clover accessions from around of the world. In this investigation, 40 genotypes have been selected and analyzed using 15 SSR primers. These primer pairs amplified 1146 polymorphic loci among the genotypes screened. The number of fragments amplified by each SSR primer combination varied from 24 for RCS3681 to 109 for RCS1729 with an average value of 80.78 per primer combination. The SSR marker data was further analyzed using cluster algorithms and Principal coordinates analysis (PCoA). The results indicated that the considerable genetic variations were discovered among the analyzed genotypes. The SSR based clustering could identify the putative pedigree types of the present red clover types of diverse origins",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Genetic diversity in red clover (trifolium pratense l.) Using SSR markers",
pages = "961-949",
number = "3",
volume = "46",
doi = "10.2298/GENSR1403949A"
}
Ahsyee, S. R., Vasiljević, S., Ćalić, I., Zorić, M., Karagić, Đ.,& Šurlan-Momirović, G.. (2014). Genetic diversity in red clover (trifolium pratense l.) Using SSR markers. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 46(3), 949-961.
https://doi.org/10.2298/GENSR1403949A
Ahsyee SR, Vasiljević S, Ćalić I, Zorić M, Karagić Đ, Šurlan-Momirović G. Genetic diversity in red clover (trifolium pratense l.) Using SSR markers. in Genetika-Belgrade. 2014;46(3):949-961.
doi:10.2298/GENSR1403949A .
Ahsyee, Salem R., Vasiljević, Sanja, Ćalić, Irena, Zorić, Miroslav, Karagić, Đura, Šurlan-Momirović, Gordana, "Genetic diversity in red clover (trifolium pratense l.) Using SSR markers" in Genetika-Belgrade, 46, no. 3 (2014):949-961,
https://doi.org/10.2298/GENSR1403949A . .
10
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7

Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers

Ahsyee, Salem R.; Al-Sloge, O.; Ćalić, Irena; Branković, Gordana; Zorić, Miroslav; Momirović, Una; Vasiljević, Sanja; Šurlan-Momirović, Gordana

(Srpsko biološko društvo, Beograd, i dr., 2013)

TY  - JOUR
AU  - Ahsyee, Salem R.
AU  - Al-Sloge, O.
AU  - Ćalić, Irena
AU  - Branković, Gordana
AU  - Zorić, Miroslav
AU  - Momirović, Una
AU  - Vasiljević, Sanja
AU  - Šurlan-Momirović, Gordana
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1253
AB  - Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The genetic diversity of nine alfalfa domesticated varietal populations was studied using thirteen RAPD primer combinations. The number of polymorphic fragments detected per primer combination ranged from 8 to 46 bands with an average of 24 bands. The number of polymorphic bands detected was from 6 (Atalia population) to 37 (Gabsia population). The lowest genetic distance was 0.058 and the highest was 0.655. The average genetic distance was (0.356). The dendrogram based on Ward's minimum variance clustering method grouped the nine populations into the two main clusters. The first group included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity of RAPD assays for detection of genetic polymorphisms is confirmed in our study, and results can be utilized in breeding practice.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers
EP  - 602
IS  - 2
SP  - 595
VL  - 65
DO  - 10.2298/ABS1302595A
ER  - 
@article{
author = "Ahsyee, Salem R. and Al-Sloge, O. and Ćalić, Irena and Branković, Gordana and Zorić, Miroslav and Momirović, Una and Vasiljević, Sanja and Šurlan-Momirović, Gordana",
year = "2013",
abstract = "Alfalfa (Medicago sativa L.) is an important forage legume in Libya. The genetic diversity of nine alfalfa domesticated varietal populations was studied using thirteen RAPD primer combinations. The number of polymorphic fragments detected per primer combination ranged from 8 to 46 bands with an average of 24 bands. The number of polymorphic bands detected was from 6 (Atalia population) to 37 (Gabsia population). The lowest genetic distance was 0.058 and the highest was 0.655. The average genetic distance was (0.356). The dendrogram based on Ward's minimum variance clustering method grouped the nine populations into the two main clusters. The first group included Fazania, Atalia, Masratia, Zawia, Denamo Ferade and Arezona. The second group was composed of Tagoria, Gabsia and Wade Alrabeh. The simplicity of RAPD assays for detection of genetic polymorphisms is confirmed in our study, and results can be utilized in breeding practice.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers",
pages = "602-595",
number = "2",
volume = "65",
doi = "10.2298/ABS1302595A"
}
Ahsyee, S. R., Al-Sloge, O., Ćalić, I., Branković, G., Zorić, M., Momirović, U., Vasiljević, S.,& Šurlan-Momirović, G.. (2013). Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(2), 595-602.
https://doi.org/10.2298/ABS1302595A
Ahsyee SR, Al-Sloge O, Ćalić I, Branković G, Zorić M, Momirović U, Vasiljević S, Šurlan-Momirović G. Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers. in Archives of Biological Sciences. 2013;65(2):595-602.
doi:10.2298/ABS1302595A .
Ahsyee, Salem R., Al-Sloge, O., Ćalić, Irena, Branković, Gordana, Zorić, Miroslav, Momirović, Una, Vasiljević, Sanja, Šurlan-Momirović, Gordana, "Genetic diversity of alfalfa domesticated varietal populations from Libyan genbank revealed by RAPD markers" in Archives of Biological Sciences, 65, no. 2 (2013):595-602,
https://doi.org/10.2298/ABS1302595A . .
3
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5

Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting

Šurlan-Momirović, Gordana; Krämer, Ilona; Bratković, Kamenko; Zorić, Miroslav; Momirović, Una; Branković, Gordana; Ćalić, Irena; Kandić, Vesna; Pržulj, Novo; Ordon, Frank; Perović, Dragan

(Društvo genetičara Srbije, Beograd, 2013)

TY  - JOUR
AU  - Šurlan-Momirović, Gordana
AU  - Krämer, Ilona
AU  - Bratković, Kamenko
AU  - Zorić, Miroslav
AU  - Momirović, Una
AU  - Branković, Gordana
AU  - Ćalić, Irena
AU  - Kandić, Vesna
AU  - Pržulj, Novo
AU  - Ordon, Frank
AU  - Perović, Dragan
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1215
AB  - Molecular diversity of 145 barley (Hordeum vulgare subsp. vulgare L.) accessions from the Serbian GenBank was assessed by single sequence repeats (SSR) markers. A set of 15 SSRs, covering all chromosomes of the diploid barley genome with 2-3 SSR markers per chromosome, with a range of 4-18 alleles per locus were used. In total, 15 loci and 119 alleles were detected, with an average of 7.93 alleles per locus. The Polymorphic information content value ranged from 0.220 to 0.782 with a mean value of 0.534. Regarding the growth habit and row type groups, gene diversity was comparatively higher for the spring (0.616) and six-rowed accessions (0.616) than for the winter and two- rowed accessions (0.322 and 0.478, respectively). Analysis of molecular variance showed that all sources of variation were significant (P  lt  0.01), but the between-group component was predominant (76.85%) for growth habit and 89.45% for row type. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on the shared allele distance (DSA) matrix estimated on the SSR data assigned the genotypes into two clusters - the first smaller consisting of the six 6-rowed spring cultivars and the second comprising six subclusters. Genotype MBR1012 was separated from all other genotypes that constitute UPGMA tree. The associations of genotypes belonging to different growth habit and row type groups were assessed using Principal Coordinate Analysis revealing separation of winter growth habit group from facultative one. The use of the STRUCTURE clustering algorithm allowed the identification of 2 subpopulations of genotypes.
AB  - Molekularni diverzitet 145 genotipova ječma (Hordeum vulgare subsp. vulgare L.) iz Gen Banke Srbije je procenjen SSR markerima. Korišćeno je SSR markera sa svih hromozoma diploidnog genoma ječma u proseku 2-3 markera po hromozomu i sa 4-18 alela po lokusu. Utvrđeno je 15 lokusa i 119 alela sa prosekom od 7.93 alela po lokusu. PIC je bio u opsegu od 0.220 do 0.782 sa prosekom 0.534. Genski diverzitet je bio veći za jare (0.616) i šestorede genotipove (0.616) u odnosu na ozime i dvorede (0.322 i 0.478). Analizom molekularne varijanse utvrđena je značajnost svih izvora variranja (P  lt  0.01), ali je međugrupna komponenta dominirala. Primenom metoda UPGMA analize zasnovane na zajedničkoj distanci alela (DSA) na osnovu SSR podataka dobijeno je grupisanje genotipove u dva klastera-jedan manji koji sadrži šest šestoredih jarih genotipova ječma i drugi koji je obuhvatao šest podklastera. Genotip MBR1012 je bio odvojen od svih ostalih genotipova. Korišćenjem metoda Osnovna Analiza Koordinata dobijeno je razdvajanje ozimih genotipova ječma od fakultativnih. Utvrđeno je postojanje dve podpopulacije genotipova primenom algoritma grupisanja STRUCTURE.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting
T1  - Molekularna karakterizacija genotipova ječma (Hordeum vulgare L.) iz gen banke Srbije SSR markerima
EP  - 180
IS  - 1
SP  - 167
VL  - 45
DO  - 10.2298/GENSR1301167S
ER  - 
@article{
author = "Šurlan-Momirović, Gordana and Krämer, Ilona and Bratković, Kamenko and Zorić, Miroslav and Momirović, Una and Branković, Gordana and Ćalić, Irena and Kandić, Vesna and Pržulj, Novo and Ordon, Frank and Perović, Dragan",
year = "2013",
abstract = "Molecular diversity of 145 barley (Hordeum vulgare subsp. vulgare L.) accessions from the Serbian GenBank was assessed by single sequence repeats (SSR) markers. A set of 15 SSRs, covering all chromosomes of the diploid barley genome with 2-3 SSR markers per chromosome, with a range of 4-18 alleles per locus were used. In total, 15 loci and 119 alleles were detected, with an average of 7.93 alleles per locus. The Polymorphic information content value ranged from 0.220 to 0.782 with a mean value of 0.534. Regarding the growth habit and row type groups, gene diversity was comparatively higher for the spring (0.616) and six-rowed accessions (0.616) than for the winter and two- rowed accessions (0.322 and 0.478, respectively). Analysis of molecular variance showed that all sources of variation were significant (P  lt  0.01), but the between-group component was predominant (76.85%) for growth habit and 89.45% for row type. Unweighted Pair Group Method with Arithmetic Mean (UPGMA) cluster analysis based on the shared allele distance (DSA) matrix estimated on the SSR data assigned the genotypes into two clusters - the first smaller consisting of the six 6-rowed spring cultivars and the second comprising six subclusters. Genotype MBR1012 was separated from all other genotypes that constitute UPGMA tree. The associations of genotypes belonging to different growth habit and row type groups were assessed using Principal Coordinate Analysis revealing separation of winter growth habit group from facultative one. The use of the STRUCTURE clustering algorithm allowed the identification of 2 subpopulations of genotypes., Molekularni diverzitet 145 genotipova ječma (Hordeum vulgare subsp. vulgare L.) iz Gen Banke Srbije je procenjen SSR markerima. Korišćeno je SSR markera sa svih hromozoma diploidnog genoma ječma u proseku 2-3 markera po hromozomu i sa 4-18 alela po lokusu. Utvrđeno je 15 lokusa i 119 alela sa prosekom od 7.93 alela po lokusu. PIC je bio u opsegu od 0.220 do 0.782 sa prosekom 0.534. Genski diverzitet je bio veći za jare (0.616) i šestorede genotipove (0.616) u odnosu na ozime i dvorede (0.322 i 0.478). Analizom molekularne varijanse utvrđena je značajnost svih izvora variranja (P  lt  0.01), ali je međugrupna komponenta dominirala. Primenom metoda UPGMA analize zasnovane na zajedničkoj distanci alela (DSA) na osnovu SSR podataka dobijeno je grupisanje genotipove u dva klastera-jedan manji koji sadrži šest šestoredih jarih genotipova ječma i drugi koji je obuhvatao šest podklastera. Genotip MBR1012 je bio odvojen od svih ostalih genotipova. Korišćenjem metoda Osnovna Analiza Koordinata dobijeno je razdvajanje ozimih genotipova ječma od fakultativnih. Utvrđeno je postojanje dve podpopulacije genotipova primenom algoritma grupisanja STRUCTURE.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting, Molekularna karakterizacija genotipova ječma (Hordeum vulgare L.) iz gen banke Srbije SSR markerima",
pages = "180-167",
number = "1",
volume = "45",
doi = "10.2298/GENSR1301167S"
}
Šurlan-Momirović, G., Krämer, I., Bratković, K., Zorić, M., Momirović, U., Branković, G., Ćalić, I., Kandić, V., Pržulj, N., Ordon, F.,& Perović, D.. (2013). Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 45(1), 167-180.
https://doi.org/10.2298/GENSR1301167S
Šurlan-Momirović G, Krämer I, Bratković K, Zorić M, Momirović U, Branković G, Ćalić I, Kandić V, Pržulj N, Ordon F, Perović D. Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting. in Genetika-Belgrade. 2013;45(1):167-180.
doi:10.2298/GENSR1301167S .
Šurlan-Momirović, Gordana, Krämer, Ilona, Bratković, Kamenko, Zorić, Miroslav, Momirović, Una, Branković, Gordana, Ćalić, Irena, Kandić, Vesna, Pržulj, Novo, Ordon, Frank, Perović, Dragan, "Molecular characterization of barley (Hordeum vulgare L.) accessions of the Serbian GeneBank by SSR fingerprinting" in Genetika-Belgrade, 45, no. 1 (2013):167-180,
https://doi.org/10.2298/GENSR1301167S . .
3
4
4

Isolation and characterization of xanthomonas euvesicatoria bacteriophages

Gagić, K.; Ivanović, Milan; Ignjatov, Maja; Ćalić, Irena; Obradović, Aleksa

(2011)

TY  - JOUR
AU  - Gagić, K.
AU  - Ivanović, Milan
AU  - Ignjatov, Maja
AU  - Ćalić, Irena
AU  - Obradović, Aleksa
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/1051
AB  - Host range, plaque morphology, thermal inactivation point, genome size and restriction fragment patterns of ten bacteriophage isolates originating from soil, pepper seed and irrigation water collected from five localities in Serbia were studied. The bacteriophage isolates were selected based on their specificity to Xanthomonas euvesicatoria, causal agent of bacterial spot of pepper. The phages had similar plaque morphology, except for two isolates producing a plaque-surrounding halo in culture of X. euvesicatoria strain KFB 189. Four phage isolates were inactivated at 70 degrees C and six at 71 degrees C. All phages had genome size of approximately 22 kb and were differentiated into four types by their EcoRI and BamHI restriction fragment patterns. Examination of two phages by transmission electron microscopy classified them as A1 morphotype members of the Myoviridae family, order Caudovirales. Although specific to X. euvesicatoria, the phages were differentiated into three groups based on their ability to lyse 59 strains of this bacterium. Adsorption rates and one-step growth curves were determined for each group representative phage isolates.
T2  - Journal of Plant Pathology
T1  - Isolation and characterization of xanthomonas euvesicatoria bacteriophages
EP  - 423
IS  - 2
SP  - 415
VL  - 93
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1051
ER  - 
@article{
author = "Gagić, K. and Ivanović, Milan and Ignjatov, Maja and Ćalić, Irena and Obradović, Aleksa",
year = "2011",
abstract = "Host range, plaque morphology, thermal inactivation point, genome size and restriction fragment patterns of ten bacteriophage isolates originating from soil, pepper seed and irrigation water collected from five localities in Serbia were studied. The bacteriophage isolates were selected based on their specificity to Xanthomonas euvesicatoria, causal agent of bacterial spot of pepper. The phages had similar plaque morphology, except for two isolates producing a plaque-surrounding halo in culture of X. euvesicatoria strain KFB 189. Four phage isolates were inactivated at 70 degrees C and six at 71 degrees C. All phages had genome size of approximately 22 kb and were differentiated into four types by their EcoRI and BamHI restriction fragment patterns. Examination of two phages by transmission electron microscopy classified them as A1 morphotype members of the Myoviridae family, order Caudovirales. Although specific to X. euvesicatoria, the phages were differentiated into three groups based on their ability to lyse 59 strains of this bacterium. Adsorption rates and one-step growth curves were determined for each group representative phage isolates.",
journal = "Journal of Plant Pathology",
title = "Isolation and characterization of xanthomonas euvesicatoria bacteriophages",
pages = "423-415",
number = "2",
volume = "93",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1051"
}
Gagić, K., Ivanović, M., Ignjatov, M., Ćalić, I.,& Obradović, A.. (2011). Isolation and characterization of xanthomonas euvesicatoria bacteriophages. in Journal of Plant Pathology, 93(2), 415-423.
https://hdl.handle.net/21.15107/rcub_fiver_1051
Gagić K, Ivanović M, Ignjatov M, Ćalić I, Obradović A. Isolation and characterization of xanthomonas euvesicatoria bacteriophages. in Journal of Plant Pathology. 2011;93(2):415-423.
https://hdl.handle.net/21.15107/rcub_fiver_1051 .
Gagić, K., Ivanović, Milan, Ignjatov, Maja, Ćalić, Irena, Obradović, Aleksa, "Isolation and characterization of xanthomonas euvesicatoria bacteriophages" in Journal of Plant Pathology, 93, no. 2 (2011):415-423,
https://hdl.handle.net/21.15107/rcub_fiver_1051 .
22
30

Use of genetic markers in breeding of perennial legumes

Šurlan-Momirović, Gordana; Katić, Slobodan; Vasiljević, Sanja; Nikolić, Zorica; Branković, Gordana; Ćalić, Irena; Milić, Dragan; Mikić, Aleksandar

(Institut za ratarstvo i povrtarstvo, Novi Sad, 2010)

TY  - JOUR
AU  - Šurlan-Momirović, Gordana
AU  - Katić, Slobodan
AU  - Vasiljević, Sanja
AU  - Nikolić, Zorica
AU  - Branković, Gordana
AU  - Ćalić, Irena
AU  - Milić, Dragan
AU  - Mikić, Aleksandar
PY  - 2010
UR  - http://fiver.ifvcns.rs/handle/123456789/849
AB  - Breeding of perennial legumes for many agronomic important traits like grain yield, persistence, longevity, resistance to diseases and pests, resistance to limiting abiotic conditions and polyploidy is more efficient and precise if genetic markers are used. Estimates based on isozyme polymorphism may underestimate overall levels of genetic variation because they are sampling only coding regions of DNA that may be conserved to maintain the function of the enzymes. The complete coverage of a genome can be achieved only by the use of molecular variability indicators (DNA polymorphism), i.e. molecular markers. Molecular markers are independent of environmental influences and can be detected in all plant development stages. The main aspects of genetic markers use in perennial legumes breeding are: germplasm characterisation, genetic linkage mapping, QTL analysis, marker assisted selection (MAS), variety identification and protection of plant breeders' rights.
AB  - Oplemenjivanje višegodišnjih leguminoza za mnoge agronomski značajne osobine kao što su prinos semena, perzistentnost, dugovečnost, otpornost na bolesti i štetočine, otpornost na limitirajuće abiotičke uslove i pojavu poliploidije efikasnije je i preciznije ako se u radu primenjuju i genetički markeri. Polimorfi zam genotipova zasnovan na profi lima izoenzima može da potceni ukupan nivo genetičke varijanse jer se odnosi na kodirajuće regione DNK koji su bili konzervirani kroz evoluciju da bi se očuvala funkcija enzima. Kompletna pokrivenost genoma može se ostvariti samo primenom pokazatelja molekularne varijabilnosti (DNK polimorfizam) tj. molekularnim markerima. Pored toga, molekularni markeri ne zavise od uslova spoljašnje sredine i mogu se detektovati u svim stadijumima fazama razvića biljaka. Glavni aspekti primene genetičkih markera u oplemenjivanju višegodišnjih leguminoza se odnose na: karakterizaciju germplazme, mapiranje vezanih gena, QTL analizu, selekciju uz pomoć markera (MAS), identifikaciju sorata i zaštitu prava oplemenjivača.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Use of genetic markers in breeding of perennial legumes
T1  - Primena genetičkih markera u oplemenjivanju višegodišnjih leguminoza
EP  - 424
IS  - 2
SP  - 417
VL  - 47
UR  - https://hdl.handle.net/21.15107/rcub_fiver_849
ER  - 
@article{
author = "Šurlan-Momirović, Gordana and Katić, Slobodan and Vasiljević, Sanja and Nikolić, Zorica and Branković, Gordana and Ćalić, Irena and Milić, Dragan and Mikić, Aleksandar",
year = "2010",
abstract = "Breeding of perennial legumes for many agronomic important traits like grain yield, persistence, longevity, resistance to diseases and pests, resistance to limiting abiotic conditions and polyploidy is more efficient and precise if genetic markers are used. Estimates based on isozyme polymorphism may underestimate overall levels of genetic variation because they are sampling only coding regions of DNA that may be conserved to maintain the function of the enzymes. The complete coverage of a genome can be achieved only by the use of molecular variability indicators (DNA polymorphism), i.e. molecular markers. Molecular markers are independent of environmental influences and can be detected in all plant development stages. The main aspects of genetic markers use in perennial legumes breeding are: germplasm characterisation, genetic linkage mapping, QTL analysis, marker assisted selection (MAS), variety identification and protection of plant breeders' rights., Oplemenjivanje višegodišnjih leguminoza za mnoge agronomski značajne osobine kao što su prinos semena, perzistentnost, dugovečnost, otpornost na bolesti i štetočine, otpornost na limitirajuće abiotičke uslove i pojavu poliploidije efikasnije je i preciznije ako se u radu primenjuju i genetički markeri. Polimorfi zam genotipova zasnovan na profi lima izoenzima može da potceni ukupan nivo genetičke varijanse jer se odnosi na kodirajuće regione DNK koji su bili konzervirani kroz evoluciju da bi se očuvala funkcija enzima. Kompletna pokrivenost genoma može se ostvariti samo primenom pokazatelja molekularne varijabilnosti (DNK polimorfizam) tj. molekularnim markerima. Pored toga, molekularni markeri ne zavise od uslova spoljašnje sredine i mogu se detektovati u svim stadijumima fazama razvića biljaka. Glavni aspekti primene genetičkih markera u oplemenjivanju višegodišnjih leguminoza se odnose na: karakterizaciju germplazme, mapiranje vezanih gena, QTL analizu, selekciju uz pomoć markera (MAS), identifikaciju sorata i zaštitu prava oplemenjivača.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Use of genetic markers in breeding of perennial legumes, Primena genetičkih markera u oplemenjivanju višegodišnjih leguminoza",
pages = "424-417",
number = "2",
volume = "47",
url = "https://hdl.handle.net/21.15107/rcub_fiver_849"
}
Šurlan-Momirović, G., Katić, S., Vasiljević, S., Nikolić, Z., Branković, G., Ćalić, I., Milić, D.,& Mikić, A.. (2010). Use of genetic markers in breeding of perennial legumes. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 47(2), 417-424.
https://hdl.handle.net/21.15107/rcub_fiver_849
Šurlan-Momirović G, Katić S, Vasiljević S, Nikolić Z, Branković G, Ćalić I, Milić D, Mikić A. Use of genetic markers in breeding of perennial legumes. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2010;47(2):417-424.
https://hdl.handle.net/21.15107/rcub_fiver_849 .
Šurlan-Momirović, Gordana, Katić, Slobodan, Vasiljević, Sanja, Nikolić, Zorica, Branković, Gordana, Ćalić, Irena, Milić, Dragan, Mikić, Aleksandar, "Use of genetic markers in breeding of perennial legumes" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 47, no. 2 (2010):417-424,
https://hdl.handle.net/21.15107/rcub_fiver_849 .