TY  - JOUR
AU  - Mesaroš, Aleksandra
AU  - Nedeljković, Marija
AU  - Danojević, Dario
AU  - Medić-Pap, Slađana
AU  - Stanković, Slaviša
AU  - Radović, Svetlana
AU  - Lozo, Jelena
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3288
AB  - With changing environmental conditions food production is becoming increasingly challenging. The production of cultivars resistant to various abiotic and/or biotic stresses may be one of the solutions. In this study, we analyzed the response of the antioxidant system in two different bell pepper genotypes, 26 susceptible and 19 tolerant, to Xanthomonas euvesicatoria when grown in the open field, greenhouse, free and isolated. The activity of four antioxidant enzymes, superoxide dismutase, glutathione reductase, catalase, and total peroxidase was highest in susceptible genotype 26 grown in isolation in the greenhouse. Both genotypes activate ascorbate peroxidase when grown in the field, suggesting that this enzyme is first in the line of defense under these growing conditions. In addition, susceptible genotype 26 had the highest concentration of ascorbic acid as a non-enzymatic defense when grown in the field. However, the carotenoid content of 26 was the lowest in the field, and the ratio of carotenoids to total chlorophyll was lowest in the field for both genotypes. In general, the susceptible genotype 26 activated the antioxidant and other defense systems more frequently than the tolerant genotype 19. Genotype 19 responded to different growth conditions with additional physiological adaptation through chlorophyll and carotenoid concentration correlated with growth location. This suggests that crossbreeding experiments to obtain genotype 19 allow the acquisition of tolerance to some abiotic stressors in addition to tolerance to the biotic stressor.
PB  - Springer
T2  - Plant Growth Regulation
T1  - Influence of growth conditions on an antioxidative system in two bell pepper genotypes differing in susceptibility to phytopathogen bacteria Xanthomonas euvesicatoria
DO  - 10.1007/s10725-023-00959-5
ER  - 

@article{
author = "Mesaroš, Aleksandra and Nedeljković, Marija and Danojević, Dario and Medić-Pap, Slađana and Stanković, Slaviša and Radović, Svetlana and Lozo, Jelena",
year = "2023",
abstract = "With changing environmental conditions food production is becoming increasingly challenging. The production of cultivars resistant to various abiotic and/or biotic stresses may be one of the solutions. In this study, we analyzed the response of the antioxidant system in two different bell pepper genotypes, 26 susceptible and 19 tolerant, to Xanthomonas euvesicatoria when grown in the open field, greenhouse, free and isolated. The activity of four antioxidant enzymes, superoxide dismutase, glutathione reductase, catalase, and total peroxidase was highest in susceptible genotype 26 grown in isolation in the greenhouse. Both genotypes activate ascorbate peroxidase when grown in the field, suggesting that this enzyme is first in the line of defense under these growing conditions. In addition, susceptible genotype 26 had the highest concentration of ascorbic acid as a non-enzymatic defense when grown in the field. However, the carotenoid content of 26 was the lowest in the field, and the ratio of carotenoids to total chlorophyll was lowest in the field for both genotypes. In general, the susceptible genotype 26 activated the antioxidant and other defense systems more frequently than the tolerant genotype 19. Genotype 19 responded to different growth conditions with additional physiological adaptation through chlorophyll and carotenoid concentration correlated with growth location. This suggests that crossbreeding experiments to obtain genotype 19 allow the acquisition of tolerance to some abiotic stressors in addition to tolerance to the biotic stressor.",
publisher = "Springer",
journal = "Plant Growth Regulation",
title = "Influence of growth conditions on an antioxidative system in two bell pepper genotypes differing in susceptibility to phytopathogen bacteria Xanthomonas euvesicatoria",
doi = "10.1007/s10725-023-00959-5"
}

Mesaroš, A., Nedeljković, M., Danojević, D., Medić-Pap, S., Stanković, S., Radović, S.,& Lozo, J.. (2023). Influence of growth conditions on an antioxidative system in two bell pepper genotypes differing in susceptibility to phytopathogen bacteria Xanthomonas euvesicatoria. in Plant Growth Regulation
Springer..
https://doi.org/10.1007/s10725-023-00959-5

Mesaroš A, Nedeljković M, Danojević D, Medić-Pap S, Stanković S, Radović S, Lozo J. Influence of growth conditions on an antioxidative system in two bell pepper genotypes differing in susceptibility to phytopathogen bacteria Xanthomonas euvesicatoria. in Plant Growth Regulation. 2023;.
doi:10.1007/s10725-023-00959-5 .

Mesaroš, Aleksandra, Nedeljković, Marija, Danojević, Dario, Medić-Pap, Slađana, Stanković, Slaviša, Radović, Svetlana, Lozo, Jelena, "Influence of growth conditions on an antioxidative system in two bell pepper genotypes differing in susceptibility to phytopathogen bacteria Xanthomonas euvesicatoria" in Plant Growth Regulation (2023),
https://doi.org/10.1007/s10725-023-00959-5 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Iličić, Renata
AU  - Milovanović, Predrag
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3286
AB  - The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.
PB  - Basel : MDPI
T2  - Microorganisms - Basel
T1  - Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia
IS  - 2
SP  - 335
VL  - 11
DO  - 10.3390/microorganisms11020335
ER  - 

@article{
author = "Jelušić, Aleksandra and Mitrović, Petar and Marković, Sanja and Iličić, Renata and Milovanović, Predrag and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this work was to identify and characterize the pectolytic bacteria responsible for the emergence of bacterial soft rot on two summer cabbage hybrids (Cheers F1 and Hippo F1) grown in the Futog locality (Baˇcka, Vojvodina), known for the five-century-long tradition of cabbage cultivation in Serbia. Symptoms manifesting as soft lesions on outer head leaves were observed during August 2021, while the inner tissues were macerated, featuring cream to black discoloration. As the affected tissue decomposed, it exuded a specific odor. Disease incidence ranged from 15% to 25%. A total of 67 isolates producing pits on crystal violet pectate (CVP) medium were characterized for their phenotypic and genotypic features. The pathogenicity was confirmed on cabbage heads. Findings yielded by the repetitive element palindromic-polymerase chain reaction (rep-PCR) technique confirmed interspecies diversity between cabbage isolates, as well as intraspecies genetic diversity within the P. carotovorum group of isolates. Based on multilocus sequence typing (MLST) using genes dnaX, mdh, icdA, and proA, five representative isolates were identified as Pectobacterium carotovorum (Cheers F1 and Hippo F1), while two were identified as Pectobacterium versatile (Hippo F1) and Pectobacterium odoriferum (Hippo F1), respectively, indicating the presence of diverse Pectobacterium species even in combined infection in the same field. Among the obtained isolates, P. carotovorum was the most prevalent species (62.69%), while P. versatile and P. odoriferum were less represented (contributing by 19.40% and 17.91%, respectively). Multilocus sequence analysis (MLSA) performed with concatenated sequences of four housekeeping genes (proA, dnaX, icdA, and mdh) and constructed a neighbor-joining phylogenetic tree enabled insight into the phylogenetic position of the Serbian cabbage Pectobacterium isolates. Bacterium P. odoriferum was found to be the most virulent species for cabbage, followed by P. versatile, while all three species had comparable virulence with respect to potato. The results obtained in this work provide a better understanding of the spreading routes and abundance of different Pectobacterium spp. in Serbia.",
publisher = "Basel : MDPI",
journal = "Microorganisms - Basel",
title = "Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia",
number = "2",
pages = "335",
volume = "11",
doi = "10.3390/microorganisms11020335"
}

Jelušić, A., Mitrović, P., Marković, S., Iličić, R., Milovanović, P., Stanković, S.,& Popović Milovanović, T.. (2023). Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia. in Microorganisms - Basel
Basel : MDPI., 11(2), 335.
https://doi.org/10.3390/microorganisms11020335

Jelušić A, Mitrović P, Marković S, Iličić R, Milovanović P, Stanković S, Popović Milovanović T. Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia. in Microorganisms - Basel. 2023;11(2):335.
doi:10.3390/microorganisms11020335 .

Jelušić, Aleksandra, Mitrović, Petar, Marković, Sanja, Iličić, Renata, Milovanović, Predrag, Stanković, Slaviša, Popović Milovanović, Tatjana, "Diversity of bacterial soft rot-causing Pectobacterium species affecting cabbage in Serbia" in Microorganisms - Basel, 11, no. 2 (2023):335,
https://doi.org/10.3390/microorganisms11020335 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Scortichini, Marco
AU  - Marković, Sanja
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Stanković, Slaviša
AU  - Popović Milovanović, Tatjana
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3737
AB  - The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.
PB  - Basel : MDPI
T2  - Microorganisms - Basel
T1  - Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia
IS  - 8
SP  - 2122
VL  - 11
DO  - 10.3390/microorganisms11082122
ER  - 

@article{
author = "Jelušić, Aleksandra and Scortichini, Marco and Marković, Sanja and Mitrović, Petar and Iličić, Renata and Stanković, Slaviša and Popović Milovanović, Tatjana",
year = "2023",
abstract = "The aim of this study was to establish a link between genetic diversity and the geographic origin of Pectobacterium strains belonging to three species—P. carotovorum, P. versatile, and P. odoriferum—isolated from cabbage in Serbia by comparing their sequences with those of strains sourced from different hosts and countries in Europe, Asia, and North America. Phylogeographic relatedness was reconstructed using the Templeton, Crandall, and Sing’s (TCS) haplotype network based on concatenated sequences of the housekeeping genes dnaX, icdA, mdh, and proA, while pairwise genetic distances were computed by applying the p-distance model. The obtained TCS haplotype networks indicated the existence of high intra-species genetic diversity among strains of all three species, as reflected in the 0.2–2.3%, 0.2–2.5%, and 0.1–1.7% genetic distance ranges obtained for P. carotovorum, P. versatile, and P. odoriferum, respectively. Five new haplotypes (denoted as HPc1–HPc5) were detected among cabbage strains of P. carotovorum, while one new haplotype was identified for both P. versatile (HPv1) and P. odoriferum (HPo1). None of the TCS haplotype networks provided evidence of significant correlation between geographic origin and the determined haplotypes, i.e., the infection origin. However, as haplotype network results are affected by the availability of sequencing data in public databases for the used genes and the number of analyzed strains, these findings may also be influenced by small sample size.",
publisher = "Basel : MDPI",
journal = "Microorganisms - Basel",
title = "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia",
number = "8",
pages = "2122",
volume = "11",
doi = "10.3390/microorganisms11082122"
}

Jelušić, A., Scortichini, M., Marković, S., Mitrović, P., Iličić, R., Stanković, S.,& Popović Milovanović, T.. (2023). Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms - Basel
Basel : MDPI., 11(8), 2122.
https://doi.org/10.3390/microorganisms11082122

Jelušić A, Scortichini M, Marković S, Mitrović P, Iličić R, Stanković S, Popović Milovanović T. Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia. in Microorganisms - Basel. 2023;11(8):2122.
doi:10.3390/microorganisms11082122 .

Jelušić, Aleksandra, Scortichini, Marco, Marković, Sanja, Mitrović, Petar, Iličić, Renata, Stanković, Slaviša, Popović Milovanović, Tatjana, "Phylogeographic Analysis of Soft-Rot-Causing Pectobacterium spp. Strains Obtained from Cabbage in Serbia" in Microorganisms - Basel, 11, no. 8 (2023):2122,
https://doi.org/10.3390/microorganisms11082122 . .

TY  - JOUR
AU  - Lozo, Jelena
AU  - Danojević, Dario
AU  - Jovanović, Živko
AU  - Nenadović, Željka
AU  - Fira, Đorđe
AU  - Stanković, Slaviša
AU  - Radović, Svetlana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/2653
AB  - We examined the effect of drought-tolerant Bacillus safensis SS-2.7 and B. thuringiensis SS-29.2 strains on the response of four (133, 274, California Wonder—CalW, and Matica) sweet pepper genotypes to water deficiency conditions. Pepper seeds were sown in pots with (treated) and without (control) bacterial strain inoculation. After four weeks of growth under controlled conditions and regular watering, drought was imposed by completely withholding watering for seven days. Under conditions of normal watering, genotype 274 showed better seedling establishment than genotype 133 and CalW, while the slowest was genotype Matica. Antioxidant enzyme activity under drought conditions was genotype and bacterial treatment-dependent. The best response to bacterial treatment in order to cope with severe drought was found in the CalW genotype, while in genotype 133, we determined even faster plant decay during water deficiency in treated seeds. Inoculated seeds of the Matica genotype did not show different antioxidant enzyme activity under normal and drought conditions. According to the obtained results, we concluded that under drought conditions, the most susceptible was genotype 274, moderate susceptibility was detected in genotype 133, and CalW and Matica were the most tolerant genotypes. Our study demonstrates (1) that drought-tolerant Bacillus strains showed a plant growth-promoting effect on some selected pepper genotypes; (2) that there were genotype-dependent antioxidant enzyme activities under drought conditions in response to treatment with a particular bacterial strain; and (3) that we could expect a genotype-dependent response during biostimulant application, especially under stress conditions.
PB  - Basel : MDPI
T2  - Horticulturae
T1  - Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains
SP  - 236
VL  - 8
DO  - 10.3390/horticulturae8030236
ER  - 

@article{
author = "Lozo, Jelena and Danojević, Dario and Jovanović, Živko and Nenadović, Željka and Fira, Đorđe and Stanković, Slaviša and Radović, Svetlana",
year = "2022",
abstract = "We examined the effect of drought-tolerant Bacillus safensis SS-2.7 and B. thuringiensis SS-29.2 strains on the response of four (133, 274, California Wonder—CalW, and Matica) sweet pepper genotypes to water deficiency conditions. Pepper seeds were sown in pots with (treated) and without (control) bacterial strain inoculation. After four weeks of growth under controlled conditions and regular watering, drought was imposed by completely withholding watering for seven days. Under conditions of normal watering, genotype 274 showed better seedling establishment than genotype 133 and CalW, while the slowest was genotype Matica. Antioxidant enzyme activity under drought conditions was genotype and bacterial treatment-dependent. The best response to bacterial treatment in order to cope with severe drought was found in the CalW genotype, while in genotype 133, we determined even faster plant decay during water deficiency in treated seeds. Inoculated seeds of the Matica genotype did not show different antioxidant enzyme activity under normal and drought conditions. According to the obtained results, we concluded that under drought conditions, the most susceptible was genotype 274, moderate susceptibility was detected in genotype 133, and CalW and Matica were the most tolerant genotypes. Our study demonstrates (1) that drought-tolerant Bacillus strains showed a plant growth-promoting effect on some selected pepper genotypes; (2) that there were genotype-dependent antioxidant enzyme activities under drought conditions in response to treatment with a particular bacterial strain; and (3) that we could expect a genotype-dependent response during biostimulant application, especially under stress conditions.",
publisher = "Basel : MDPI",
journal = "Horticulturae",
title = "Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains",
pages = "236",
volume = "8",
doi = "10.3390/horticulturae8030236"
}

Lozo, J., Danojević, D., Jovanović, Ž., Nenadović, Ž., Fira, Đ., Stanković, S.,& Radović, S.. (2022). Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains. in Horticulturae
Basel : MDPI., 8, 236.
https://doi.org/10.3390/horticulturae8030236

Lozo J, Danojević D, Jovanović Ž, Nenadović Ž, Fira Đ, Stanković S, Radović S. Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains. in Horticulturae. 2022;8:236.
doi:10.3390/horticulturae8030236 .

Lozo, Jelena, Danojević, Dario, Jovanović, Živko, Nenadović, Željka, Fira, Đorđe, Stanković, Slaviša, Radović, Svetlana, "Genotype-dependent antioxidative response of four sweet pepper cultivars to water deficiency as affected by drought-tolerant Bacillus safensis SS-2.7 and Bacillus thuringiensis SS-29.2 strains" in Horticulturae, 8 (2022):236,
https://doi.org/10.3390/horticulturae8030236 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Peeters, Kelly
AU  - Miklavčić Višnjevec, Ana
AU  - Tavzes, Črtomir
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3750
AB  - Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC–MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.
PB  - Elsevier
T2  - Biological Control
T1  - Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates
SP  - 104695
VL  - 160
DO  - 10.1016/j.biocontrol.2021.104695
ER  - 

@article{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Peeters, Kelly and Miklavčić Višnjevec, Ana and Tavzes, Črtomir and Stanković, Slaviša and Berić, Tanja",
year = "2021",
abstract = "Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC–MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.",
publisher = "Elsevier",
journal = "Biological Control",
title = "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates",
pages = "104695",
volume = "160",
doi = "10.1016/j.biocontrol.2021.104695"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Peeters, K., Miklavčić Višnjevec, A., Tavzes, Č., Stanković, S.,& Berić, T.. (2021). Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control
Elsevier., 160, 104695.
https://doi.org/10.1016/j.biocontrol.2021.104695

Jelušić A, Popović T, Dimkić I, Mitrović P, Peeters K, Miklavčić Višnjevec A, Tavzes Č, Stanković S, Berić T. Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control. 2021;160:104695.
doi:10.1016/j.biocontrol.2021.104695 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Peeters, Kelly, Miklavčić Višnjevec, Ana, Tavzes, Črtomir, Stanković, Slaviša, Berić, Tanja, "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates" in Biological Control, 160 (2021):104695,
https://doi.org/10.1016/j.biocontrol.2021.104695 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marjanović-Jeromela, Ana
AU  - Popović, Tatjana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2097
AB  - Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia
EP  - 49
IS  - 1
SP  - 35
VL  - 70
DO  - 10.1111/ppa.13273
ER  - 

@article{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marjanović-Jeromela, Ana and Popović, Tatjana",
year = "2021",
abstract = "Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia",
pages = "49-35",
number = "1",
volume = "70",
doi = "10.1111/ppa.13273"
}

Jelušić, A., Berić, T., Mitrović, P., Dimkić, I., Stanković, S., Marjanović-Jeromela, A.,& Popović, T.. (2021). New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia. in Plant Pathology
Wiley, Hoboken., 70(1), 35-49.
https://doi.org/10.1111/ppa.13273

Jelušić A, Berić T, Mitrović P, Dimkić I, Stanković S, Marjanović-Jeromela A, Popović T. New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia. in Plant Pathology. 2021;70(1):35-49.
doi:10.1111/ppa.13273 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marjanović-Jeromela, Ana, Popović, Tatjana, "New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia" in Plant Pathology, 70, no. 1 (2021):35-49,
https://doi.org/10.1111/ppa.13273 . .

TY  - CONF
AU  - Nenadović, Željka
AU  - Danojević, Dario
AU  - Jovanović, Živko
AU  - Stanković, Slaviša
AU  - Fira, Đorđe
AU  - Radović, Svetlana
AU  - Lozo, Jelena
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3061
AB  - The worldwide increase in human population followed with industrialization led to environmental changes as a result of water, soil and air pollution. It is believed that one way to address this problem is to change the existing agricultural approaches and to use plant growth promoting bacteria (PGPB) instead of fertilizers and pesticides. The present investigation was aimed to evaluate the influence of PGPB on sweet pepper seed priming. Pepper seeds (Capsicum annuum L.) from six different genotype, 115 and 116 (peppers for spice), 261 (sivri type), 133, 258, and 274 (kapia type) were primed with 10-9 CFU/ml of Bacillus safensis (SS-2.7), B. thuringiensis (SS-29.2), Pseudomonas putida (4.3) and P. protegens CHA0 strains. Seeds were primed during one hour and after three weeks root and seedlings length, and the total mass of the pepper seedlings were analyzed. One and two-way analysis of variance (ANOVA) and Least Significant Difference test (LSD test) with a 0.05 probability level was used for statistical analysis.
PB  - Belgrade : Serbian Genetic Society
C3  - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
T1  - Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains
EP  - 274
SP  - 274
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3061
ER  - 

@conference{
author = "Nenadović, Željka and Danojević, Dario and Jovanović, Živko and Stanković, Slaviša and Fira, Đorđe and Radović, Svetlana and Lozo, Jelena",
year = "2019",
abstract = "The worldwide increase in human population followed with industrialization led to environmental changes as a result of water, soil and air pollution. It is believed that one way to address this problem is to change the existing agricultural approaches and to use plant growth promoting bacteria (PGPB) instead of fertilizers and pesticides. The present investigation was aimed to evaluate the influence of PGPB on sweet pepper seed priming. Pepper seeds (Capsicum annuum L.) from six different genotype, 115 and 116 (peppers for spice), 261 (sivri type), 133, 258, and 274 (kapia type) were primed with 10-9 CFU/ml of Bacillus safensis (SS-2.7), B. thuringiensis (SS-29.2), Pseudomonas putida (4.3) and P. protegens CHA0 strains. Seeds were primed during one hour and after three weeks root and seedlings length, and the total mass of the pepper seedlings were analyzed. One and two-way analysis of variance (ANOVA) and Least Significant Difference test (LSD test) with a 0.05 probability level was used for statistical analysis.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja",
title = "Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains",
pages = "274-274",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3061"
}

Nenadović, Ž., Danojević, D., Jovanović, Ž., Stanković, S., Fira, Đ., Radović, S.,& Lozo, J.. (2019). Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
Belgrade : Serbian Genetic Society., 274-274.
https://hdl.handle.net/21.15107/rcub_fiver_3061

Nenadović Ž, Danojević D, Jovanović Ž, Stanković S, Fira Đ, Radović S, Lozo J. Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:274-274.
https://hdl.handle.net/21.15107/rcub_fiver_3061 .

Nenadović, Željka, Danojević, Dario, Jovanović, Živko, Stanković, Slaviša, Fira, Đorđe, Radović, Svetlana, Lozo, Jelena, "Genotypic variation in the response of sweet pepper on seed priming with selected Bacillus and Pseudomonas strains" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):274-274,
https://hdl.handle.net/21.15107/rcub_fiver_3061 .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3056
AB  - Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from different localities in Serbia, with the aim to examine their genetic features, through sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained sequences were checked for homology with strains available in NCBI database.
PB  - Belgrade : Serbian Genetic Society
C3  - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
T1  - Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape
EP  - 163
SP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3056
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from different localities in Serbia, with the aim to examine their genetic features, through sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained sequences were checked for homology with strains available in NCBI database.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja",
title = "Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape",
pages = "163-163",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3056"
}

Jelušić, A., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
Belgrade : Serbian Genetic Society., 163-163.
https://hdl.handle.net/21.15107/rcub_fiver_3056

Jelušić A, Berić T, Mitrović P, Marković S, Stanković S, Popović T. Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:163-163.
https://hdl.handle.net/21.15107/rcub_fiver_3056 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):163-163,
https://hdl.handle.net/21.15107/rcub_fiver_3056 .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Marjanović-Jeromela, Ana
AU  - Nikolić, I
AU  - Stanković, S.
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1850
AB  - The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia
EP  - 1457
IS  - 8
SP  - 1448
VL  - 68
DO  - 10.1111/ppa.13064
ER  - 

@article{
author = "Popović, Tatjana and Mitrović, Petar and Jelušić, Aleksandra and Dimkić, Ivica and Marjanović-Jeromela, Ana and Nikolić, I and Stanković, S.",
year = "2019",
abstract = "The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia",
pages = "1457-1448",
number = "8",
volume = "68",
doi = "10.1111/ppa.13064"
}

Popović, T., Mitrović, P., Jelušić, A., Dimkić, I., Marjanović-Jeromela, A., Nikolić, I.,& Stanković, S.. (2019). Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology
Wiley, Hoboken., 68(8), 1448-1457.
https://doi.org/10.1111/ppa.13064

Popović T, Mitrović P, Jelušić A, Dimkić I, Marjanović-Jeromela A, Nikolić I, Stanković S. Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology. 2019;68(8):1448-1457.
doi:10.1111/ppa.13064 .

Popović, Tatjana, Mitrović, Petar, Jelušić, Aleksandra, Dimkić, Ivica, Marjanović-Jeromela, Ana, Nikolić, I, Stanković, S., "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia" in Plant Pathology, 68, no. 8 (2019):1448-1457,
https://doi.org/10.1111/ppa.13064 . .