TY  - JOUR
AU  - Bagi, Ferenc
AU  - Iličić, Renata
AU  - Jevtić, Radivoje
AU  - Orbović, Branka
AU  - Savić, Zagorka
AU  - Suman, Michele
AU  - Tóth, Beáta
AU  - Berėnyi, Attila
AU  - Popović, Tatjana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3178
AB  - Toxigenic potential of four and one isolate of A. alternata and A. tenuissima, respectively, on durum wheat cultivar Dušan (Triticum durum L.) and common wheat cultivar Barbee (T. vulgare L.) were tested. Three different wheat / isolate genotype combinations were used for artificial inoculation of grains under laboratory conditions and toxins production. Alternaria toxins alternariol (AOH), alternariol monomethyl ether (AME), ten-toxin (TEN), tenuazonic acid (TeA) and altenuen (ALT) concentrations were determined by LC-MS/MS. Cultivar Barbee proved to be a more suitable substrate for toxin production, whereby AOH, AME and TeA were present in highest concentrations. These results under- line the possibility of fungal infection and mycotoxin production by Alternaria species in field and under storage conditions. Further research is needed for official regulation of acceptable levels of Alternaria mycotoxins in food and feed.
AB  - U radu je ispitan toksigeni potencijal izolata vrsta iz roda Alternaria – A. alternata i A. tenuissima u laboratorijskim uslovima na durum pšenici sorte „Dušan” (Triticum durum L.), kao i na sorti pšenice „Barbee” (T. vulgare L.). Tokom veštačke inokulacije u laboratoriji korišćene su tri različite kombinacije genotip pšenice/izolati. Putem LC-MS/MS metode ispitan je sadržaj alternaria toksina alternariola (AOH), alternariol mometiletra (AME), tentoksina (TEN), tenuazonične kiseline (TeA) i altenuena (ALT). Sorta „Barbi” pokazala se kao najpogodniji supstrat za produkciju alternaria toksina. U ispitivanim uzorcima utvrđena je najviša koncentracija toksina AOH, AME i TeA. Rezultati ovih istraživanja ukazuju na mogućnost ostvarenja infekcije strnih žita od strane Alternaria vrsta tokom skladištenja i na rizik od potencijalne kontaminacije od strane mikotoksina i ulaska otrovnih jedinjenja u lanac ishrane. Dalja istraživanja i potreba zvanične regulacije maksimalno dozvoljenih količina Alternaria toksina u cilju su smanjenja rizika od trovanja sekundarnim metabolitima koje stvaraju gljive iz roda Alternaria.
PB  - Novi Sad : Matica srpska
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Toxigenic potential of Alternaria species from cereals
T1  - oksigeni potencijal vrsta iz roda Alternaria izolovanih sa strnih žita
EP  - 45
SP  - 39
VL  - 142
DO  - 10.2298/ZMSPN2242039B
ER  - 

@article{
author = "Bagi, Ferenc and Iličić, Renata and Jevtić, Radivoje and Orbović, Branka and Savić, Zagorka and Suman, Michele and Tóth, Beáta and Berėnyi, Attila and Popović, Tatjana",
year = "2022",
abstract = "Toxigenic potential of four and one isolate of A. alternata and A. tenuissima, respectively, on durum wheat cultivar Dušan (Triticum durum L.) and common wheat cultivar Barbee (T. vulgare L.) were tested. Three different wheat / isolate genotype combinations were used for artificial inoculation of grains under laboratory conditions and toxins production. Alternaria toxins alternariol (AOH), alternariol monomethyl ether (AME), ten-toxin (TEN), tenuazonic acid (TeA) and altenuen (ALT) concentrations were determined by LC-MS/MS. Cultivar Barbee proved to be a more suitable substrate for toxin production, whereby AOH, AME and TeA were present in highest concentrations. These results under- line the possibility of fungal infection and mycotoxin production by Alternaria species in field and under storage conditions. Further research is needed for official regulation of acceptable levels of Alternaria mycotoxins in food and feed., U radu je ispitan toksigeni potencijal izolata vrsta iz roda Alternaria – A. alternata i A. tenuissima u laboratorijskim uslovima na durum pšenici sorte „Dušan” (Triticum durum L.), kao i na sorti pšenice „Barbee” (T. vulgare L.). Tokom veštačke inokulacije u laboratoriji korišćene su tri različite kombinacije genotip pšenice/izolati. Putem LC-MS/MS metode ispitan je sadržaj alternaria toksina alternariola (AOH), alternariol mometiletra (AME), tentoksina (TEN), tenuazonične kiseline (TeA) i altenuena (ALT). Sorta „Barbi” pokazala se kao najpogodniji supstrat za produkciju alternaria toksina. U ispitivanim uzorcima utvrđena je najviša koncentracija toksina AOH, AME i TeA. Rezultati ovih istraživanja ukazuju na mogućnost ostvarenja infekcije strnih žita od strane Alternaria vrsta tokom skladištenja i na rizik od potencijalne kontaminacije od strane mikotoksina i ulaska otrovnih jedinjenja u lanac ishrane. Dalja istraživanja i potreba zvanične regulacije maksimalno dozvoljenih količina Alternaria toksina u cilju su smanjenja rizika od trovanja sekundarnim metabolitima koje stvaraju gljive iz roda Alternaria.",
publisher = "Novi Sad : Matica srpska",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Toxigenic potential of Alternaria species from cereals, oksigeni potencijal vrsta iz roda Alternaria izolovanih sa strnih žita",
pages = "45-39",
volume = "142",
doi = "10.2298/ZMSPN2242039B"
}

Bagi, F., Iličić, R., Jevtić, R., Orbović, B., Savić, Z., Suman, M., Tóth, B., Berėnyi, A.,& Popović, T.. (2022). Toxigenic potential of Alternaria species from cereals. in Zbornik Matice srpske za prirodne nauke
Novi Sad : Matica srpska., 142, 39-45.
https://doi.org/10.2298/ZMSPN2242039B

Bagi F, Iličić R, Jevtić R, Orbović B, Savić Z, Suman M, Tóth B, Berėnyi A, Popović T. Toxigenic potential of Alternaria species from cereals. in Zbornik Matice srpske za prirodne nauke. 2022;142:39-45.
doi:10.2298/ZMSPN2242039B .

Bagi, Ferenc, Iličić, Renata, Jevtić, Radivoje, Orbović, Branka, Savić, Zagorka, Suman, Michele, Tóth, Beáta, Berėnyi, Attila, Popović, Tatjana, "Toxigenic potential of Alternaria species from cereals" in Zbornik Matice srpske za prirodne nauke, 142 (2022):39-45,
https://doi.org/10.2298/ZMSPN2242039B . .

TY  - CONF
AU  - Bagi, Ferenc
AU  - Iličić, Renata
AU  - Savić, Zagorka
AU  - Jevtić, Radivoje
AU  - Orbović, Branka
AU  - Suman, Michele
AU  - Toth, Beáta
AU  - Berėnyi, Attila
AU  - Popović, Tatjana
PY  - 2022
UR  - http://fiver.ifvcns.rs/handle/123456789/3208
AB  - Toxigenic potential of A. alternata and A. tenuissima isolates on durum wheat grains of cultivar Dušan (Triticum durum L.) and common wheat cultivar Barbee (T. vulgare L.) were tested under laboratory conditions. A. alternata and A. tenuissima isolates were used for inoculate three different wheat genotype/fungal isolates combination. Alternaria toxins alternariol (AOH), alternariol monomethyl ether (AME), tentoxin (TEN), tenuazonic acid (TeA) and altenuen (ALT) were determined by LC-MS/MS. Grains of cultivar Barbee proved to be the best substrate for toxin production. Among toxins, AOH, AME and TeA were present in highest concentration. The results underline the possibility of fungal infection and mycotoxin production by Alternaria species both in field and storage conditions. Further research is needed for official regulation of acceptable levels of Alternaria mycotoxins in food and feed.
PB  - Novi Sad : Matica srpska
C3  - Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.
T1  - Toxigenic potential of Alternaria species from cereals
EP  - 21
SP  - 21
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3208
ER  - 

@conference{
author = "Bagi, Ferenc and Iličić, Renata and Savić, Zagorka and Jevtić, Radivoje and Orbović, Branka and Suman, Michele and Toth, Beáta and Berėnyi, Attila and Popović, Tatjana",
year = "2022",
abstract = "Toxigenic potential of A. alternata and A. tenuissima isolates on durum wheat grains of cultivar Dušan (Triticum durum L.) and common wheat cultivar Barbee (T. vulgare L.) were tested under laboratory conditions. A. alternata and A. tenuissima isolates were used for inoculate three different wheat genotype/fungal isolates combination. Alternaria toxins alternariol (AOH), alternariol monomethyl ether (AME), tentoxin (TEN), tenuazonic acid (TeA) and altenuen (ALT) were determined by LC-MS/MS. Grains of cultivar Barbee proved to be the best substrate for toxin production. Among toxins, AOH, AME and TeA were present in highest concentration. The results underline the possibility of fungal infection and mycotoxin production by Alternaria species both in field and storage conditions. Further research is needed for official regulation of acceptable levels of Alternaria mycotoxins in food and feed.",
publisher = "Novi Sad : Matica srpska",
journal = "Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.",
title = "Toxigenic potential of Alternaria species from cereals",
pages = "21-21",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3208"
}

Bagi, F., Iličić, R., Savić, Z., Jevtić, R., Orbović, B., Suman, M., Toth, B., Berėnyi, A.,& Popović, T.. (2022). Toxigenic potential of Alternaria species from cereals. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.
Novi Sad : Matica srpska., 21-21.
https://hdl.handle.net/21.15107/rcub_fiver_3208

Bagi F, Iličić R, Savić Z, Jevtić R, Orbović B, Suman M, Toth B, Berėnyi A, Popović T. Toxigenic potential of Alternaria species from cereals. in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022.. 2022;:21-21.
https://hdl.handle.net/21.15107/rcub_fiver_3208 .

Bagi, Ferenc, Iličić, Renata, Savić, Zagorka, Jevtić, Radivoje, Orbović, Branka, Suman, Michele, Toth, Beáta, Berėnyi, Attila, Popović, Tatjana, "Toxigenic potential of Alternaria species from cereals" in Knjiga rezimea, 7. međunarodni naučni skup "Mikologija, mikotoksikologija i mikoze", Novi Sad, 2-3. jun 2022. (2022):21-21,
https://hdl.handle.net/21.15107/rcub_fiver_3208 .

TY  - JOUR
AU  - Trkulja, Vojislav
AU  - Mitrović, Petar
AU  - Mihić Salapura, Jelena
AU  - Iličić, Renata
AU  - Ćurković, Bojana
AU  - Đalović, Ivica
AU  - Popović, Tatjana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3749
AB  - At the beginning of July 2020, 3-month-old carrot plants (Daucus carota L. variety Maestro F1) grown in a commercial field 1.2 ha in size at the Begeč locality (45°14′30.38″ N, 19°36′44.82″ E) in the southern part of the Bačka region, Vojvodina, Serbia, exhibited symptoms of yellowing and reddish leaf discoloration. At the end of July, leaves on the infected plants became bronze and purplish, and their shoots and roots were stunted due to dehydration, with pronounced proliferation. In some cases, the damage was so extensive that it led to plant decay. The disease incidence of 0.5 to 1% recorded early in July rapidly escalated, reaching 10 to 15% in the first 10 days of August. The observed symptoms resembled those caused by ‘Candidatus Liberibacter solanacearum’ (CaLso), a phloem-limited proteobacterium (Munyaneza et al. 2015). To detect and identify CaLso, 15 symptomatic diseased and five asymptomatic healthy carrot plants were subjected to conventional polymerase chain reaction (PCR) using two primer sets specific to CaLso, and positive PCR products were further sequenced using commercial facilities (Macrogen Europe). Total DNA was extracted from petiole and root tissues using a commercial kit (Qiagen DNEasy Plant Mini Kit) following the manufacturer-recommended protocol. In the first PCR, using the Lso TX 16/23 F/R primer pair that targets the 16S-23S rRNA intergenic spacer region specific to CaLso (Ravindran et al. 2011), all 15 diseased samples yielded a band of 383-bp size. After sequencing, 100% homology was noted among tested isolates; therefore, one isolate coded as 1842/20 was chosen as representative and was deposited in NCBI GenBank under accession number MT948144. BLAST analysis showed 99.70% identity of Serbian carrot isolates with those of the CaLso isolate 80022 originating from celery seed in Slovenia or Italy (accession no. KY619977) (Monger and Jeffries 2018), as well as 99.41% identity with isolate GBBC_Clso_03 from carrot in Belgium (accession no. MH734515) and 98.22% identity with the sequence of the CaLso reference strain NZ082226 (accession no. EU834130) isolated from tomato in New Zealand (Liefting et al. 2009). In the second PCR, species-specific forward primer LsoF empirically designed at the signature region of the 16S rRNA sequence of CaLso (Li et al. 2009) in combination with the universal Liberibacter reverse primer OI2c (Jagoueix et al. 1996) yielded a target of 1,163-bp size in all 15 diseased symptomatic carrot samples. Representative isolate 1842/20 was deposited in NCBI GenBank under accession number MW187524. Based on the nucleotide BLAST analysis, the sequence of Serbian carrot isolate showed 100% identity with CaLso strains 16-004 and 16-011 originating from carrot in Finland (accession nos. MG701014 and MG701015, respectively) and 99.64% identity with CaLso reference strain NZ082226 (accession no. EU834130). Five healthy asymptomatic carrot plant samples were negative for the presence of CaLso in both PCR tests employed in this work. To our knowledge, this is the first report of CaLso causing the disease in carrot in Serbia. These results suggest a wider distribution of this pathogen than previously reported in Europe. In 2014, psyllid Bactericera trigonica (Hemiptera, Triozidae) was described for the first time as a potential vector for CaLso transmission in few localities, including Begeč (Jerinić-Prodanović 2014). Considering that its vectors are presently unidentified, certain aspects of CaLso genomics, diversity, epidemiology, and vector dynamics will be studied further in future investigations.
PB  - American Phytopathological Society
T2  - Plant Disease
T1  - First Report of 'Candidatus Liberibacter solanacearum' on Carrot in Serbia
IS  - 4
SP  - 1188
VL  - 105
DO  - 10.1094/PDIS-11-20-2384-PDN
ER  - 

@article{
author = "Trkulja, Vojislav and Mitrović, Petar and Mihić Salapura, Jelena and Iličić, Renata and Ćurković, Bojana and Đalović, Ivica and Popović, Tatjana",
year = "2021",
abstract = "At the beginning of July 2020, 3-month-old carrot plants (Daucus carota L. variety Maestro F1) grown in a commercial field 1.2 ha in size at the Begeč locality (45°14′30.38″ N, 19°36′44.82″ E) in the southern part of the Bačka region, Vojvodina, Serbia, exhibited symptoms of yellowing and reddish leaf discoloration. At the end of July, leaves on the infected plants became bronze and purplish, and their shoots and roots were stunted due to dehydration, with pronounced proliferation. In some cases, the damage was so extensive that it led to plant decay. The disease incidence of 0.5 to 1% recorded early in July rapidly escalated, reaching 10 to 15% in the first 10 days of August. The observed symptoms resembled those caused by ‘Candidatus Liberibacter solanacearum’ (CaLso), a phloem-limited proteobacterium (Munyaneza et al. 2015). To detect and identify CaLso, 15 symptomatic diseased and five asymptomatic healthy carrot plants were subjected to conventional polymerase chain reaction (PCR) using two primer sets specific to CaLso, and positive PCR products were further sequenced using commercial facilities (Macrogen Europe). Total DNA was extracted from petiole and root tissues using a commercial kit (Qiagen DNEasy Plant Mini Kit) following the manufacturer-recommended protocol. In the first PCR, using the Lso TX 16/23 F/R primer pair that targets the 16S-23S rRNA intergenic spacer region specific to CaLso (Ravindran et al. 2011), all 15 diseased samples yielded a band of 383-bp size. After sequencing, 100% homology was noted among tested isolates; therefore, one isolate coded as 1842/20 was chosen as representative and was deposited in NCBI GenBank under accession number MT948144. BLAST analysis showed 99.70% identity of Serbian carrot isolates with those of the CaLso isolate 80022 originating from celery seed in Slovenia or Italy (accession no. KY619977) (Monger and Jeffries 2018), as well as 99.41% identity with isolate GBBC_Clso_03 from carrot in Belgium (accession no. MH734515) and 98.22% identity with the sequence of the CaLso reference strain NZ082226 (accession no. EU834130) isolated from tomato in New Zealand (Liefting et al. 2009). In the second PCR, species-specific forward primer LsoF empirically designed at the signature region of the 16S rRNA sequence of CaLso (Li et al. 2009) in combination with the universal Liberibacter reverse primer OI2c (Jagoueix et al. 1996) yielded a target of 1,163-bp size in all 15 diseased symptomatic carrot samples. Representative isolate 1842/20 was deposited in NCBI GenBank under accession number MW187524. Based on the nucleotide BLAST analysis, the sequence of Serbian carrot isolate showed 100% identity with CaLso strains 16-004 and 16-011 originating from carrot in Finland (accession nos. MG701014 and MG701015, respectively) and 99.64% identity with CaLso reference strain NZ082226 (accession no. EU834130). Five healthy asymptomatic carrot plant samples were negative for the presence of CaLso in both PCR tests employed in this work. To our knowledge, this is the first report of CaLso causing the disease in carrot in Serbia. These results suggest a wider distribution of this pathogen than previously reported in Europe. In 2014, psyllid Bactericera trigonica (Hemiptera, Triozidae) was described for the first time as a potential vector for CaLso transmission in few localities, including Begeč (Jerinić-Prodanović 2014). Considering that its vectors are presently unidentified, certain aspects of CaLso genomics, diversity, epidemiology, and vector dynamics will be studied further in future investigations.",
publisher = "American Phytopathological Society",
journal = "Plant Disease",
title = "First Report of 'Candidatus Liberibacter solanacearum' on Carrot in Serbia",
number = "4",
pages = "1188",
volume = "105",
doi = "10.1094/PDIS-11-20-2384-PDN"
}

Trkulja, V., Mitrović, P., Mihić Salapura, J., Iličić, R., Ćurković, B., Đalović, I.,& Popović, T.. (2021). First Report of 'Candidatus Liberibacter solanacearum' on Carrot in Serbia. in Plant Disease
American Phytopathological Society., 105(4), 1188.
https://doi.org/10.1094/PDIS-11-20-2384-PDN

Trkulja V, Mitrović P, Mihić Salapura J, Iličić R, Ćurković B, Đalović I, Popović T. First Report of 'Candidatus Liberibacter solanacearum' on Carrot in Serbia. in Plant Disease. 2021;105(4):1188.
doi:10.1094/PDIS-11-20-2384-PDN .

Trkulja, Vojislav, Mitrović, Petar, Mihić Salapura, Jelena, Iličić, Renata, Ćurković, Bojana, Đalović, Ivica, Popović, Tatjana, "First Report of 'Candidatus Liberibacter solanacearum' on Carrot in Serbia" in Plant Disease, 105, no. 4 (2021):1188,
https://doi.org/10.1094/PDIS-11-20-2384-PDN . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Dimkić, Ivica
AU  - Mitrović, Petar
AU  - Peeters, Kelly
AU  - Miklavčić Višnjevec, Ana
AU  - Tavzes, Črtomir
AU  - Stanković, Slaviša
AU  - Berić, Tanja
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/3750
AB  - Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC–MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.
PB  - Elsevier
T2  - Biological Control
T1  - Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates
SP  - 104695
VL  - 160
DO  - 10.1016/j.biocontrol.2021.104695
ER  - 

@article{
author = "Jelušić, Aleksandra and Popović, Tatjana and Dimkić, Ivica and Mitrović, Petar and Peeters, Kelly and Miklavčić Višnjevec, Ana and Tavzes, Črtomir and Stanković, Slaviša and Berić, Tanja",
year = "2021",
abstract = "Plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) was recently described as a pathogen of winter oilseed rape in Serbia. A metabarcoding approach was used to study bacterial community composition changes in the phyllosphere and rhizosphere of different winter oilseed rape types (lines, cultivars, and hybrids) naturally infected with Xcc. This study also aimed to examine the potential of indigenous Bacillus and Pseudomonas isolates to suppress Xcc. Infection with Xcc leads to depletion of bacterial diversity in the plants' phyllosphere and explicitly decreasing the abundance of genera such as Exiguobacterium, Massilia, and Pantoea with potentially beneficial properties. Strains identified as Bacillus velezensis X5-2, Bacillus megaterium X6-3, and Pseudomonas orientalis X2-1P obtained from oilseed rape phyllosphere were found to be effective against Xcc in vitro and in vivo when applied as a whole-culture and as a cell-free supernatant. The greenhouse in vivo tests on winter oilseed rape plants with three selected biocontrol strains lead to a disease reduction of 82.37% and 72.47% in preventive and curative treatments, respectively. Genetic screening showed potential for the biosynthesis of surfactin, kurstakin, bacillomycin D, and iturin in B. velezensis X5-2, as well as surfactin and kurstakin in B. megaterium X6-3. Gene phcA encoding phenazine-1-carboxylic acid was detected in P. orientalis X2-1P. The chemical composition of ethyl acetate and benzene extracts of three biocontrol strains obtained by GC–MS and HPLC-ESI-qTOF/MS analyses indicates numerous volatile organic compounds (alkenes, benzenes, carboxylic acids, indoles, pyrazines, etc.), lipopeptides, and/or antibiotics, for many of which antimicrobial potential is proven. We assume that this wide range of metabolites is responsible for the exhibited biocontrol activity against Xcc. These results recommend all three biocontrol strains for further studies for use as agents for biocontrol of bacterial blight-like disease caused by Xcc of oilseed rape.",
publisher = "Elsevier",
journal = "Biological Control",
title = "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates",
pages = "104695",
volume = "160",
doi = "10.1016/j.biocontrol.2021.104695"
}

Jelušić, A., Popović, T., Dimkić, I., Mitrović, P., Peeters, K., Miklavčić Višnjevec, A., Tavzes, Č., Stanković, S.,& Berić, T.. (2021). Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control
Elsevier., 160, 104695.
https://doi.org/10.1016/j.biocontrol.2021.104695

Jelušić A, Popović T, Dimkić I, Mitrović P, Peeters K, Miklavčić Višnjevec A, Tavzes Č, Stanković S, Berić T. Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates. in Biological Control. 2021;160:104695.
doi:10.1016/j.biocontrol.2021.104695 .

Jelušić, Aleksandra, Popović, Tatjana, Dimkić, Ivica, Mitrović, Petar, Peeters, Kelly, Miklavčić Višnjevec, Ana, Tavzes, Črtomir, Stanković, Slaviša, Berić, Tanja, "Changes in the winter oilseed rape microbiome affected by Xanthomonas campestris pv. campestris and biocontrol potential of the indigenous Bacillus and Pseudomonas isolates" in Biological Control, 160 (2021):104695,
https://doi.org/10.1016/j.biocontrol.2021.104695 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Kosovac, Andrea
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2243
AB  - ‘Candidatus Phytoplasma solani’, known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during august 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for ‘Ca. P. solani’ presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stol11 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmp1. Combining molecular tools PCR/RFLP and sequencing reviled two ‘Ca. P. solani’ multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the ‘Ca. P. solani’ diversity on celery, but supplemented with genotyping of the vmp1 gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of ‘Ca. P. solani’ affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia.
AB  - ‘Candidatus Phytoplasma solani’, poznata po trivijalnom nazivu stolbur fitoplazma, biljni je patogen koji inficira brojne kulture u Srbiji. Biljke celera sa izraženim žutilom i hlorozom listova, sporadično i sa nekrozom tkiva, uočene su u avgustu 2020. u Futogu, predgrađu Novog Sada u Vojvodini. Ukupno 12 uzoraka celera, 8 simptomatičnih i 4 asimptomatične biljke, analizirane su na prisustvo ‘Ca. P. solani’. Svi simptomatični uzorci su se pokazali inficiranim stolbur fitoplazmom na osnovu analize stol11 gena i dalje su molekularno okarakterisani na 3 gena: tuf, stamp i vmp1. Kombinacijom molekularnih tehnika PCR/RFLP i sekvenciranja utvrđeno je prisustvo dva ‘Ca. P. solani’ multigenska genotipa, tuf-b/Rqg31/V14 i tuf-b/Rpm35/V14, prisutnih u po četiri uzorka. Dobijeni rezultati genotipizacije uzoraka stolbur fitoplazme odgovaraju prethodnim podacima o diverzitetu ovog patogena na celeru u Srbiji i dopunjeni su sa genotipizacijom vmp1 gena. Pojava stolbur fitoplazme u Futogu je vezana za tuf-b epidemiologiju, koja uglavnom izvire iz korovske vrste Convolvulus arvensis (poponac), koja je bila prisutna u istraživanom zasadu celera i mogla je biti izvor inokuluma. Vizuelna procena pojave simptoma pokazala je 10-15% prisustva simptomatičnih biljaka celera, nepravilno raspoređenih unutar zasada što ukazuje da je odgovorni vektor najverovatnije cikada Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) asocirana sa poponcem, najznačajniji vektor stolbur fitoplazme u Srbiji.
PB  - Novi Sad : Institut za ratarstvo i povrtarstvo
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog
T1  - Molekularna karakterizacija 'Candidatus Phytoplasma solani' na celeru: studija u Futogu
EP  - 71
IS  - 2
SP  - 66
VL  - 58
DO  - 10.5937/ratpov58-33227
ER  - 

@article{
author = "Popović, Tatjana and Mitrović, Petar and Kosovac, Andrea",
year = "2021",
abstract = "‘Candidatus Phytoplasma solani’, known by its trivial name stolbur phytoplasma, is a plant pathogen infecting numerous crops in Serbia. Celery plants with prominent leaf yellowing and chlorosis, sporadically with tissue necrosis, were observed during august 2020 in Futog, situated in Novi Sad suburbia in Vojvodina. Total of 12 sampled celery plants, 8 symptomatic and 4 asymptomatic ones, were analysed for ‘Ca. P. solani’ presence. All symptomatic celery plants were infected with stolbur phytoplasma according to the stol11 gene detection and therefore were further subjected to multigene molecular characterization on three genes: tuf, stamp and vmp1. Combining molecular tools PCR/RFLP and sequencing reviled two ‘Ca. P. solani’ multilocus genotypes in celery: tuf-b/Rqg31/V14 and tuf-b/Rpm35/V14, present in 4 samples each. Obtained results of the strain genotyping are in concordance with previous data on the ‘Ca. P. solani’ diversity on celery, but supplemented with genotyping of the vmp1 gene. Outbreak of stolbur phytoplasma in the assessed locality in Futog is linked to tuf-b epidemiological cycle correlated in Serbia mainly with weed Convolvulus arvensis which was present in the subjected celery plot, and could have been the phytoplasma inoculum source. Visual evaluation of the symptom occurrence suggests on 10-15% of ‘Ca. P. solani’ affected celery plants scattered throughout the plot corresponding to the pathogen dispersal in crop by cixiid planthoper Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) associated with C. arvensis, main vector of stolbur phytoplasma in Serbia., ‘Candidatus Phytoplasma solani’, poznata po trivijalnom nazivu stolbur fitoplazma, biljni je patogen koji inficira brojne kulture u Srbiji. Biljke celera sa izraženim žutilom i hlorozom listova, sporadično i sa nekrozom tkiva, uočene su u avgustu 2020. u Futogu, predgrađu Novog Sada u Vojvodini. Ukupno 12 uzoraka celera, 8 simptomatičnih i 4 asimptomatične biljke, analizirane su na prisustvo ‘Ca. P. solani’. Svi simptomatični uzorci su se pokazali inficiranim stolbur fitoplazmom na osnovu analize stol11 gena i dalje su molekularno okarakterisani na 3 gena: tuf, stamp i vmp1. Kombinacijom molekularnih tehnika PCR/RFLP i sekvenciranja utvrđeno je prisustvo dva ‘Ca. P. solani’ multigenska genotipa, tuf-b/Rqg31/V14 i tuf-b/Rpm35/V14, prisutnih u po četiri uzorka. Dobijeni rezultati genotipizacije uzoraka stolbur fitoplazme odgovaraju prethodnim podacima o diverzitetu ovog patogena na celeru u Srbiji i dopunjeni su sa genotipizacijom vmp1 gena. Pojava stolbur fitoplazme u Futogu je vezana za tuf-b epidemiologiju, koja uglavnom izvire iz korovske vrste Convolvulus arvensis (poponac), koja je bila prisutna u istraživanom zasadu celera i mogla je biti izvor inokuluma. Vizuelna procena pojave simptoma pokazala je 10-15% prisustva simptomatičnih biljaka celera, nepravilno raspoređenih unutar zasada što ukazuje da je odgovorni vektor najverovatnije cikada Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha) asocirana sa poponcem, najznačajniji vektor stolbur fitoplazme u Srbiji.",
publisher = "Novi Sad : Institut za ratarstvo i povrtarstvo",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog, Molekularna karakterizacija 'Candidatus Phytoplasma solani' na celeru: studija u Futogu",
pages = "71-66",
number = "2",
volume = "58",
doi = "10.5937/ratpov58-33227"
}

Popović, T., Mitrović, P.,& Kosovac, A.. (2021). Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Novi Sad : Institut za ratarstvo i povrtarstvo., 58(2), 66-71.
https://doi.org/10.5937/ratpov58-33227

Popović T, Mitrović P, Kosovac A. Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2021;58(2):66-71.
doi:10.5937/ratpov58-33227 .

Popović, Tatjana, Mitrović, Petar, Kosovac, Andrea, "Molecular characterization of 'Candidatus Phytoplasma solani' in celery: Case study in Futog" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 58, no. 2 (2021):66-71,
https://doi.org/10.5937/ratpov58-33227 . .

TY  - JOUR
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marjanović-Jeromela, Ana
AU  - Popović, Tatjana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2097
AB  - Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia
EP  - 49
IS  - 1
SP  - 35
VL  - 70
DO  - 10.1111/ppa.13273
ER  - 

@article{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marjanović-Jeromela, Ana and Popović, Tatjana",
year = "2021",
abstract = "Winter oilseed rape has recently been described as a new host for the phytopathogenic bacteriumXanthomonas campestrispv.campestris(Xcc) in Serbia. In order to provide information about the genetic structure of this bacterium's population and its pathogenic features on this crop, a collection of 65 winter oilseed rape Xcc isolates obtained over a 5-year period (2014-2018) from different Serbian localities was examined. The presence of intrapathovar diversity within the tested isolates was confirmed by repetitive element PCR (rep-PCR), revealing 14, 10, and 4 different profiles for BOX, ERIC, and REP primers, respectively. A multilocus sequence typing (MLST) approach separated the isolates into seven groups on a phylogenetic tree. The PubMLST non-redundant database was used to compare sequences of four housekeeping genes from isolates with 75 strains ofXanthomonasspp. to determine nucleotide changes caused by host-pathogen coevolution. Five allelic profile combinations (ST3, ST5, ST9, ST26, and ST47) were determined, with ST47 being the most frequent. Pathogenicity and host range tests showed higher virulence of the tested isolates on winter oilseed rape than on otherBrassica oleraceahosts (broccoli, cabbage, cauliflower, collard greens, kale, and kohlrabi). The most aggressive isolates were NCPPB4679, NCPPB4680, and Xc361, belonging to genotype ST26. Fifty oilseed rape lines, cultivars, and hybrids showed susceptibility to the three Xcc isolates tested (of high, low, and moderate virulence), indicating that there was no resistance to the bacterium. The results provide significant information about diversification of Xcc isolates and their evolution toward specialization for winter oilseed rape.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia",
pages = "49-35",
number = "1",
volume = "70",
doi = "10.1111/ppa.13273"
}

Jelušić, A., Berić, T., Mitrović, P., Dimkić, I., Stanković, S., Marjanović-Jeromela, A.,& Popović, T.. (2021). New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia. in Plant Pathology
Wiley, Hoboken., 70(1), 35-49.
https://doi.org/10.1111/ppa.13273

Jelušić A, Berić T, Mitrović P, Dimkić I, Stanković S, Marjanović-Jeromela A, Popović T. New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia. in Plant Pathology. 2021;70(1):35-49.
doi:10.1111/ppa.13273 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marjanović-Jeromela, Ana, Popović, Tatjana, "New insights into the genetic diversity of Xanthomonas campestris pv. campestris isolates from winter oilseed rape in Serbia" in Plant Pathology, 70, no. 1 (2021):35-49,
https://doi.org/10.1111/ppa.13273 . .

TY  - JOUR
AU  - Mitrović, Petar
AU  - Đalović, Ivica
AU  - Kiprovski, Biljana
AU  - Veljović-Jovanović, Sonja
AU  - Trkulja, Vojislav
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2078
AB  - The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.
PB  - Basel : MDPI
T2  - Plants-Basel
T1  - Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani
IS  - 2
VL  - 10
DO  - 10.3390/plants10020337
ER  - 

@article{
author = "Mitrović, Petar and Đalović, Ivica and Kiprovski, Biljana and Veljović-Jovanović, Sonja and Trkulja, Vojislav and Jelušić, Aleksandra and Popović, Tatjana",
year = "2021",
abstract = "The present study examined the effects of Candidatus Phytoplasma solani infection on antioxidative metabolism in leaves and roots of carrot (Daucus carota L.). Disease symptoms appeared at the end of June in the form of the chlorosis on some of the leaves, which became intensely red one week later, while the previously healthy leaves from the same branch becme chlorotic. A few days later, all leaves from the infected leaf branch were intensely red. Infected plants also had slower growth compared to the healthy ones with fewer leaf branches developed. The roots of infected plants were less developed, seared, or gummy with or without brown-colored root hair. The presence of the pathogen was detected by sequencing the 16S rRNA. National Center for Biotechnology Information (NCBI) BLAST analyses of the obtained sequence revealed 100% identity of tested strain with deposited Ca. Phytoplasma solani strains from various countries and hosts, all belonging to the "stolbur" group (16SrXII-A). Identity of 99.74% was found when the tested Serbian strain (MF503627) was compared with the reference stolbur strain STOL11 (AF248959). The oxidative damage of membranes in carrot cells was accompanied by a decrease in the content of photosynthetic pigments. Furthermore, for the determination of specific scavenging properties of the extracts, in vitro antioxidant assay was performed. In phytoplasma-infected carrot leaves, there was a greater reduction in the level of glutathione content (GSH); however; flavonoids and anthocyanidins seem to be responsible for the accompanied increased antioxidative capacity against hydroxyl radical and hydrogen peroxide.",
publisher = "Basel : MDPI",
journal = "Plants-Basel",
title = "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani",
number = "2",
volume = "10",
doi = "10.3390/plants10020337"
}

Mitrović, P., Đalović, I., Kiprovski, B., Veljović-Jovanović, S., Trkulja, V., Jelušić, A.,& Popović, T.. (2021). Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel
Basel : MDPI., 10(2).
https://doi.org/10.3390/plants10020337

Mitrović P, Đalović I, Kiprovski B, Veljović-Jovanović S, Trkulja V, Jelušić A, Popović T. Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani. in Plants-Basel. 2021;10(2).
doi:10.3390/plants10020337 .

Mitrović, Petar, Đalović, Ivica, Kiprovski, Biljana, Veljović-Jovanović, Sonja, Trkulja, Vojislav, Jelušić, Aleksandra, Popović, Tatjana, "Oxidative Stress and Antioxidative Activity in Leaves and Roots of Carrot Plants Induced by Candidatus Phytoplasma Solani" in Plants-Basel, 10, no. 2 (2021),
https://doi.org/10.3390/plants10020337 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Mitrović, Petar
AU  - Iličić, Renata
AU  - Marković, Sanja
PY  - 2020
UR  - http://fiver.ifvcns.rs/handle/123456789/1989
AB  - Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which affect proper cabbage growth, leading to head weight and quality losses and thereby drastically reducing its marketing value. The pathogen is genetically diverse, which is evident from the presence of eleven races worldwide and more than thirty combinations of allelic profiles. Therefore, this study aimed to determine the allelic profiles of Serbian cabbage Xcc strains obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was first amplified using polymerase chain reaction (PCR) with four housekeeping genes - P-XdnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were finally used to determine allelic profiles. Allelic profiles were determined by comparison with 33 Xcc strains obtained from different hosts and regions, whose allelic profiles had been determined previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profile determination and Phyloviz software for constructing a minimum spanning tree. The obtained allelic profile of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and rpoD genes, respectively. This profile is assigned as sequence type 2 (ST2) and it coincides with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No connection between sequence type (ST) and the host was detected.
AB  - Xanthomonas campestris pv. campestris (Xcc), prouzrokovač crne truleži kupusa (Brassica oleracea var. capitata L.) svrstava se među najvažnije fitopatogene bakterije koje utiču na pravilno razviće kupusa, dovodeći do gubitka težine glavice i njenog kvaliteta i na taj način drastično smanjuje njegovu ekonomsku vrednost. Ovaj patogen je genetički heterogen, što se ogleda kroz prisustvo dokazanih jedanaest rasa i više od trideset kombinacija alelskih profila širom sveta. Zbog svega navedenog, cilj ovog rada je determinacija alelskih profila Xcc sojeva poreklom sa kupusa prikupljenih 2014. godine. Analiza je vršena kod tri reprezentativna Xcc soja čija je DNK amplifikovana primenom lančane reakcije polimeraze (PCR) sa četiri konzervativna gena - dnaK, fyuA, gyrB i rpoD, a zatim sekvencirana i korišćena za determinaciju alelskih profila. Alelski profili su određivani poređenjem sa 33 Xcc soja izolovana sa različitih domaćina i regiona, čiji su alelski profili prethodno utvrđeni. Nonredundant baza podataka (NRDB) od pubMLST je korišćena za determinaciju alaleskih profila, a Phyloviz softver za konstrukciju Minimum Spanning stabla. Dobijeni alelski profil za sve Xcc sojeve sa kupusa iz Srbije je 1, 3, 1, 1 za gene dnaK, fyuA, gyrB i rpoD, redom. Ovaj profil je označen kao tip sekvence 2 (ST2) i podudara se sa portugalskim B. oleracea Xcc sojem CPBF 213 poreklom sa B. oleracea var. costata. Veza između tipa sekvence (ST) i biljke domaćina nije pronađena.
PB  - Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd
T2  - Pesticidi i fitomedicina
T1  - Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage
T1  - Alelski profil izolata Xanthomonas campestris pv. campestris sa kupusa u Srbiji
EP  - 26
IS  - 1
SP  - 19
VL  - 35
DO  - 10.2298/PIF2001019P
ER  - 

@article{
author = "Popović, Tatjana and Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Marković, Sanja",
year = "2020",
abstract = "Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which affect proper cabbage growth, leading to head weight and quality losses and thereby drastically reducing its marketing value. The pathogen is genetically diverse, which is evident from the presence of eleven races worldwide and more than thirty combinations of allelic profiles. Therefore, this study aimed to determine the allelic profiles of Serbian cabbage Xcc strains obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was first amplified using polymerase chain reaction (PCR) with four housekeeping genes - P-XdnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were finally used to determine allelic profiles. Allelic profiles were determined by comparison with 33 Xcc strains obtained from different hosts and regions, whose allelic profiles had been determined previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profile determination and Phyloviz software for constructing a minimum spanning tree. The obtained allelic profile of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and rpoD genes, respectively. This profile is assigned as sequence type 2 (ST2) and it coincides with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No connection between sequence type (ST) and the host was detected., Xanthomonas campestris pv. campestris (Xcc), prouzrokovač crne truleži kupusa (Brassica oleracea var. capitata L.) svrstava se među najvažnije fitopatogene bakterije koje utiču na pravilno razviće kupusa, dovodeći do gubitka težine glavice i njenog kvaliteta i na taj način drastično smanjuje njegovu ekonomsku vrednost. Ovaj patogen je genetički heterogen, što se ogleda kroz prisustvo dokazanih jedanaest rasa i više od trideset kombinacija alelskih profila širom sveta. Zbog svega navedenog, cilj ovog rada je determinacija alelskih profila Xcc sojeva poreklom sa kupusa prikupljenih 2014. godine. Analiza je vršena kod tri reprezentativna Xcc soja čija je DNK amplifikovana primenom lančane reakcije polimeraze (PCR) sa četiri konzervativna gena - dnaK, fyuA, gyrB i rpoD, a zatim sekvencirana i korišćena za determinaciju alelskih profila. Alelski profili su određivani poređenjem sa 33 Xcc soja izolovana sa različitih domaćina i regiona, čiji su alelski profili prethodno utvrđeni. Nonredundant baza podataka (NRDB) od pubMLST je korišćena za determinaciju alaleskih profila, a Phyloviz softver za konstrukciju Minimum Spanning stabla. Dobijeni alelski profil za sve Xcc sojeve sa kupusa iz Srbije je 1, 3, 1, 1 za gene dnaK, fyuA, gyrB i rpoD, redom. Ovaj profil je označen kao tip sekvence 2 (ST2) i podudara se sa portugalskim B. oleracea Xcc sojem CPBF 213 poreklom sa B. oleracea var. costata. Veza između tipa sekvence (ST) i biljke domaćina nije pronađena.",
publisher = "Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd",
journal = "Pesticidi i fitomedicina",
title = "Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage, Alelski profil izolata Xanthomonas campestris pv. campestris sa kupusa u Srbiji",
pages = "26-19",
number = "1",
volume = "35",
doi = "10.2298/PIF2001019P"
}

Popović, T., Jelušić, A., Mitrović, P., Iličić, R.,& Marković, S.. (2020). Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage. in Pesticidi i fitomedicina
Institut za pesticide i zaštitu životne sredine, Beograd i Društvo za zaštitu bilja Srbije, Beograd., 35(1), 19-26.
https://doi.org/10.2298/PIF2001019P

Popović T, Jelušić A, Mitrović P, Iličić R, Marković S. Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage. in Pesticidi i fitomedicina. 2020;35(1):19-26.
doi:10.2298/PIF2001019P .

Popović, Tatjana, Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Marković, Sanja, "Allelic profile of Serbian Xanthomonas campestris pv. campestris isolates from cabbage" in Pesticidi i fitomedicina, 35, no. 1 (2020):19-26,
https://doi.org/10.2298/PIF2001019P . .

TY  - JOUR
AU  - Iličić, Renata
AU  - Popović, Tatjana
AU  - Vlajić, Slobodan
AU  - Ognjanov, Vladislav
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1926
AB  - In recent years, sweet and sour cherry production in Serbia has increased. Under Serbian agroecological conditions, pathogens causing leaf diseases threaten the success of sweet and sour cherry production. In the period 2012-2019, the health status of cherries was monitored in more than 30 locations. Depending on the production system, the following leaf pathogens were identified: Blumeriella jaapii, Wilsonomyces carpophilus, Mycosphaerella cerasella, Phoma prunicola, Podosphaera clandestina and Pseudomonas syringae pv. syringae and morsprunorum race 1. Leaf pathogens caused premature defoliation, which adversely affected bud formation for the next growing season and increased susceptibility to freezing. Therefore, attention should be focused on correct leaf pathogen identification, and proper selection, application and rotation of fungicides.
AB  - Poslednjih godina proizvodnja trešnje i višnje u Srbiji je povećana. U našim agroekološkim uslovima, patogeni lista ugrožavaju proizvodnju ovih perspektivnih voćnih vrsta. U periodu 2012-2019. godine praćeno je zdravstveno stanje trešnje i višnje, na više od 30 lokaliteta. avisno od sistema proizvodnje identifikovani su sledeći patogeni lista: Blumeriella jaapii, Wilsonomyces carpophilus, Mycosphaerella cerasella, Phoma prunicola, Podosphaera clandestina, Pseudomonas siringae pv. syringae i morsprunorum rasa 1. Patogeni lista izazivaju prevremenu defolijaciju, što negativno utiče na formiranje pupoljaka za narednu vegetaciju i povećanu osetljivost na izmrzavanje. Stoga pažnju treba usmeriti na ispravnu identifikaciju patogena, pravilan izbor i rotaciju fungicida različitog mehanizma delovanja, kao i optimalno vreme za njihovu primenu.
PB  - Univerzitet u Kragujevcu - Agronomski fakultet, Čačak
T2  - Acta agriculturae Serbica
T1  - Foliar pathogens of sweet and sour cherry in Serbia
T1  - Patogeni lista trešnje i višnje u Srbiji
EP  - 118
IS  - 48
SP  - 107
VL  - 24
DO  - 10.5937/AASer1948107I
ER  - 

@article{
author = "Iličić, Renata and Popović, Tatjana and Vlajić, Slobodan and Ognjanov, Vladislav",
year = "2019",
abstract = "In recent years, sweet and sour cherry production in Serbia has increased. Under Serbian agroecological conditions, pathogens causing leaf diseases threaten the success of sweet and sour cherry production. In the period 2012-2019, the health status of cherries was monitored in more than 30 locations. Depending on the production system, the following leaf pathogens were identified: Blumeriella jaapii, Wilsonomyces carpophilus, Mycosphaerella cerasella, Phoma prunicola, Podosphaera clandestina and Pseudomonas syringae pv. syringae and morsprunorum race 1. Leaf pathogens caused premature defoliation, which adversely affected bud formation for the next growing season and increased susceptibility to freezing. Therefore, attention should be focused on correct leaf pathogen identification, and proper selection, application and rotation of fungicides., Poslednjih godina proizvodnja trešnje i višnje u Srbiji je povećana. U našim agroekološkim uslovima, patogeni lista ugrožavaju proizvodnju ovih perspektivnih voćnih vrsta. U periodu 2012-2019. godine praćeno je zdravstveno stanje trešnje i višnje, na više od 30 lokaliteta. avisno od sistema proizvodnje identifikovani su sledeći patogeni lista: Blumeriella jaapii, Wilsonomyces carpophilus, Mycosphaerella cerasella, Phoma prunicola, Podosphaera clandestina, Pseudomonas siringae pv. syringae i morsprunorum rasa 1. Patogeni lista izazivaju prevremenu defolijaciju, što negativno utiče na formiranje pupoljaka za narednu vegetaciju i povećanu osetljivost na izmrzavanje. Stoga pažnju treba usmeriti na ispravnu identifikaciju patogena, pravilan izbor i rotaciju fungicida različitog mehanizma delovanja, kao i optimalno vreme za njihovu primenu.",
publisher = "Univerzitet u Kragujevcu - Agronomski fakultet, Čačak",
journal = "Acta agriculturae Serbica",
title = "Foliar pathogens of sweet and sour cherry in Serbia, Patogeni lista trešnje i višnje u Srbiji",
pages = "118-107",
number = "48",
volume = "24",
doi = "10.5937/AASer1948107I"
}

Iličić, R., Popović, T., Vlajić, S.,& Ognjanov, V.. (2019). Foliar pathogens of sweet and sour cherry in Serbia. in Acta agriculturae Serbica
Univerzitet u Kragujevcu - Agronomski fakultet, Čačak., 24(48), 107-118.
https://doi.org/10.5937/AASer1948107I

Iličić R, Popović T, Vlajić S, Ognjanov V. Foliar pathogens of sweet and sour cherry in Serbia. in Acta agriculturae Serbica. 2019;24(48):107-118.
doi:10.5937/AASer1948107I .

Iličić, Renata, Popović, Tatjana, Vlajić, Slobodan, Ognjanov, Vladislav, "Foliar pathogens of sweet and sour cherry in Serbia" in Acta agriculturae Serbica, 24, no. 48 (2019):107-118,
https://doi.org/10.5937/AASer1948107I . .

TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/3056
AB  - Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from different localities in Serbia, with the aim to examine their genetic features, through sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained sequences were checked for homology with strains available in NCBI database.
PB  - Belgrade : Serbian Genetic Society
C3  - Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
T1  - Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape
EP  - 163
SP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3056
ER  - 

@conference{
author = "Jelušić, Aleksandra and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Oilseed rape (Brassica napus L.) is a hybrid species within Brassicaceae family, significant for its oil-rich seed, mainly used for vegetable oil and biodiesel production, but also for consumption as livestock feed. Yield of oilseed rape and other cruciferous crops can be reduced due to different plant pathogenic bacteria, fungi and pests. One of the most significant bacteria affecting crucifers is Xanthomonas campestris pv. campestris (Xcc) causing black rot disease. Xcc isolates from diseased winter oilseed rape plants showing bacterial blight symptom on leaves were collected in nine-year period (2010-2018), from different localities in Serbia, with the aim to examine their genetic features, through sequencing of housekeeping genes. DNA of the obtained isolates was therefore amplified with six primers (dnaK, fyuA, gapA, gyrB, lepA, rpoD) and sent for sequencing. The obtained sequences were checked for homology with strains available in NCBI database.",
publisher = "Belgrade : Serbian Genetic Society",
journal = "Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja",
title = "Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape",
pages = "163-163",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3056"
}

Jelušić, A., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja
Belgrade : Serbian Genetic Society., 163-163.
https://hdl.handle.net/21.15107/rcub_fiver_3056

Jelušić A, Berić T, Mitrović P, Marković S, Stanković S, Popović T. Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape. in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja. 2019;:163-163.
https://hdl.handle.net/21.15107/rcub_fiver_3056 .

Jelušić, Aleksandra, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "Genetic diversity of Serbian isolates of Xanthomonas campestris pv. campestris originated from winter oilseed rape" in Book of Abstracts, 6th Congress of the Serbian Genetic Society, 13-17 October 2019, Vrnjačka Banja (2019):163-163,
https://hdl.handle.net/21.15107/rcub_fiver_3056 .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Marjanović-Jeromela, Ana
AU  - Nikolić, I
AU  - Stanković, S.
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1850
AB  - The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.
PB  - Wiley, Hoboken
T2  - Plant Pathology
T1  - Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia
EP  - 1457
IS  - 8
SP  - 1448
VL  - 68
DO  - 10.1111/ppa.13064
ER  - 

@article{
author = "Popović, Tatjana and Mitrović, Petar and Jelušić, Aleksandra and Dimkić, Ivica and Marjanović-Jeromela, Ana and Nikolić, I and Stanković, S.",
year = "2019",
abstract = "The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar-specific primer set was used for fast preliminary identification. In repetitive sequence-based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP-PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP-PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap-1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three-nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host-pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.",
publisher = "Wiley, Hoboken",
journal = "Plant Pathology",
title = "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia",
pages = "1457-1448",
number = "8",
volume = "68",
doi = "10.1111/ppa.13064"
}

Popović, T., Mitrović, P., Jelušić, A., Dimkić, I., Marjanović-Jeromela, A., Nikolić, I.,& Stanković, S.. (2019). Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology
Wiley, Hoboken., 68(8), 1448-1457.
https://doi.org/10.1111/ppa.13064

Popović T, Mitrović P, Jelušić A, Dimkić I, Marjanović-Jeromela A, Nikolić I, Stanković S. Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. in Plant Pathology. 2019;68(8):1448-1457.
doi:10.1111/ppa.13064 .

Popović, Tatjana, Mitrović, Petar, Jelušić, Aleksandra, Dimkić, Ivica, Marjanović-Jeromela, Ana, Nikolić, I, Stanković, S., "Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia" in Plant Pathology, 68, no. 8 (2019):1448-1457,
https://doi.org/10.1111/ppa.13064 . .

TY  - JOUR
AU  - Kiprovski, Biljana
AU  - Đalović, Ivica
AU  - Adamović, Dušan
AU  - Mitrović, Petar
AU  - Marjanović-Jeromela, Ana
AU  - Malenčić, Ðorđe
AU  - Popović, Tatjana
PY  - 2018
UR  - http://fiver.ifvcns.rs/handle/123456789/1791
AB  - The aim of the present paper was to study the response of Oenothera biennis L. to 'Candidatus Phytoplasma solani' (Stolbur) infection by analyzing total sugars, polyphenols, photosynthetic pigments content and the antioxidant capacity in leaves and roots of healthy and infected plants. The infection caused a significant increase in peroxidation of lipids, phenylalanine ammonia-lyase activity, total sugar, polyphenols and anthocyanins content (2.8, 2.6, 1.8, 1.4, 6.8 fold, respectively), as well as a decrease in photosynthetic pigments (2-6 fold) and total flavonoids (1.5 fold) in the leaves of Oe. biennis. Changes in these parameters were insignificant in the roots except for the total polyphenols content that was 2.7 times higher in the infected ones. Reduced gluthatione content in both tested organs was not affected by the infection (3.7 and 1.7 mu mol/g fresh weight of leaves and roots, respectively). The elevated content of total sugars, flavonoids and polyphenols, as well as the reduction of photosynthetic pigments and anthocyanins in infected plants are indicative of changes in the metabolism of Oe. biennis affected by the Stolbur phytoplasma. In addition to reduction of chlorophyll and carotenoids, the phytoplasma accelerated leaf senescence. Plants responded to the infection via enhanced superoxide anion scavenging, even though this reaction did not prevent, apparently, membrane damage in analysed leaves. This investigation presents new data on the effect of a phytoplasma infection on its host.
PB  - Springer, New York
T2  - Journal of Plant Pathology
T1  - Biochemical changes in Oenothera biennis plants infected by 'Candidatus Phytoplasma solani'
EP  - 214
IS  - 2
SP  - 209
VL  - 100
DO  - 10.1007/S42161-018-0068-0
ER  - 

@article{
author = "Kiprovski, Biljana and Đalović, Ivica and Adamović, Dušan and Mitrović, Petar and Marjanović-Jeromela, Ana and Malenčić, Ðorđe and Popović, Tatjana",
year = "2018",
abstract = "The aim of the present paper was to study the response of Oenothera biennis L. to 'Candidatus Phytoplasma solani' (Stolbur) infection by analyzing total sugars, polyphenols, photosynthetic pigments content and the antioxidant capacity in leaves and roots of healthy and infected plants. The infection caused a significant increase in peroxidation of lipids, phenylalanine ammonia-lyase activity, total sugar, polyphenols and anthocyanins content (2.8, 2.6, 1.8, 1.4, 6.8 fold, respectively), as well as a decrease in photosynthetic pigments (2-6 fold) and total flavonoids (1.5 fold) in the leaves of Oe. biennis. Changes in these parameters were insignificant in the roots except for the total polyphenols content that was 2.7 times higher in the infected ones. Reduced gluthatione content in both tested organs was not affected by the infection (3.7 and 1.7 mu mol/g fresh weight of leaves and roots, respectively). The elevated content of total sugars, flavonoids and polyphenols, as well as the reduction of photosynthetic pigments and anthocyanins in infected plants are indicative of changes in the metabolism of Oe. biennis affected by the Stolbur phytoplasma. In addition to reduction of chlorophyll and carotenoids, the phytoplasma accelerated leaf senescence. Plants responded to the infection via enhanced superoxide anion scavenging, even though this reaction did not prevent, apparently, membrane damage in analysed leaves. This investigation presents new data on the effect of a phytoplasma infection on its host.",
publisher = "Springer, New York",
journal = "Journal of Plant Pathology",
title = "Biochemical changes in Oenothera biennis plants infected by 'Candidatus Phytoplasma solani'",
pages = "214-209",
number = "2",
volume = "100",
doi = "10.1007/S42161-018-0068-0"
}

Kiprovski, B., Đalović, I., Adamović, D., Mitrović, P., Marjanović-Jeromela, A., Malenčić, Ð.,& Popović, T.. (2018). Biochemical changes in Oenothera biennis plants infected by 'Candidatus Phytoplasma solani'. in Journal of Plant Pathology
Springer, New York., 100(2), 209-214.
https://doi.org/10.1007/S42161-018-0068-0

Kiprovski B, Đalović I, Adamović D, Mitrović P, Marjanović-Jeromela A, Malenčić Ð, Popović T. Biochemical changes in Oenothera biennis plants infected by 'Candidatus Phytoplasma solani'. in Journal of Plant Pathology. 2018;100(2):209-214.
doi:10.1007/S42161-018-0068-0 .

Kiprovski, Biljana, Đalović, Ivica, Adamović, Dušan, Mitrović, Petar, Marjanović-Jeromela, Ana, Malenčić, Ðorđe, Popović, Tatjana, "Biochemical changes in Oenothera biennis plants infected by 'Candidatus Phytoplasma solani'" in Journal of Plant Pathology, 100, no. 2 (2018):209-214,
https://doi.org/10.1007/S42161-018-0068-0 . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović, Tatjana
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Petrović, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1628
AB  - Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3).
AB  - Crni luk (Allium cepa L.) spada u najznačajnije povrtarske biljne vrste u Srbiji. Gaji se na površini od oko 20.000 hektara. Tokom rutinske kontrole kvaliteta semena crnog luka u 2014. godini, primećena je pojava Fusarium sp. u visokom procentu - od 28%. Cilj rada bio je izolacija i identifikacija Fusarium sp. na osnovu morfoloških i molekularnih karakteristika patogena. Uzorci semena luka sakupljeni su s različitih lokaliteta i iz velikog broja skladišta u Vojvodini. Nakon izolacije patogena odabrano je i morfološki okarakterisano 25 izolata Fusarium sp. Izolacija DNK izvršena je direktno iz micelija gljive (100 mg), korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Amplifikacija DNK ciljanog gena (translation elongation factor EF-1α gene) obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1α gena, koji je deponovan u NCBI bazu podataka pod brojem KP658211 (Fusarium sp. FIESC 3).
PB  - Matica srpska, Novi Sad
T2  - Zbornik Matice srpske za prirodne nauke
T1  - Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)
T1  - Identifikacija i filogenetska analiza Fusarium sp. FIESC 3 prouzrokovača truleži semena crnog luka (Allium cepa L.)
EP  - 17
IS  - 132
SP  - 9
DO  - 10.2298/ZMSPN1732009I
ER  - 

@article{
author = "Ignjatov, Maja and Popović, Tatjana and Milošević, Dragana and Nikolić, Zorica and Petrović, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko",
year = "2017",
abstract = "Onion (Allium cepa L.) is one of the most important vegetable crops in Serbia, where it is grown on an approximate surface of 20,000 ha. During the routine quality control analysis of onion seed in 2014, fungal infection was observed in an average of 28% of the seed. The objective of this paper was to isolate, determine, and identify Fusarium sp. based on the pathogen's morphological and molecular characteristics. Onion seed samples were collected from different localities in the region of Vojvodina. To obtain a DNA sequence-based identification, a total DNA of the 25 isolates was extracted directly from the mycelium (100 mg wet weight), with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in 25 tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1α gene, which was deposited in the NCBI GenBank database under accession number KP658211 (Fusarium sp. FIESC3)., Crni luk (Allium cepa L.) spada u najznačajnije povrtarske biljne vrste u Srbiji. Gaji se na površini od oko 20.000 hektara. Tokom rutinske kontrole kvaliteta semena crnog luka u 2014. godini, primećena je pojava Fusarium sp. u visokom procentu - od 28%. Cilj rada bio je izolacija i identifikacija Fusarium sp. na osnovu morfoloških i molekularnih karakteristika patogena. Uzorci semena luka sakupljeni su s različitih lokaliteta i iz velikog broja skladišta u Vojvodini. Nakon izolacije patogena odabrano je i morfološki okarakterisano 25 izolata Fusarium sp. Izolacija DNK izvršena je direktno iz micelija gljive (100 mg), korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Amplifikacija DNK ciljanog gena (translation elongation factor EF-1α gene) obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1α gena, koji je deponovan u NCBI bazu podataka pod brojem KP658211 (Fusarium sp. FIESC 3).",
publisher = "Matica srpska, Novi Sad",
journal = "Zbornik Matice srpske za prirodne nauke",
title = "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.), Identifikacija i filogenetska analiza Fusarium sp. FIESC 3 prouzrokovača truleži semena crnog luka (Allium cepa L.)",
pages = "17-9",
number = "132",
doi = "10.2298/ZMSPN1732009I"
}

Ignjatov, M., Popović, T., Milošević, D., Nikolić, Z., Petrović, G., Gvozdanović-Varga, J.,& Ivanović, Ž.. (2017). Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke
Matica srpska, Novi Sad.(132), 9-17.
https://doi.org/10.2298/ZMSPN1732009I

Ignjatov M, Popović T, Milošević D, Nikolić Z, Petrović G, Gvozdanović-Varga J, Ivanović Ž. Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.). in Zbornik Matice srpske za prirodne nauke. 2017;(132):9-17.
doi:10.2298/ZMSPN1732009I .

Ignjatov, Maja, Popović, Tatjana, Milošević, Dragana, Nikolić, Zorica, Petrović, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, "Identification and phylogenetic analysis of Fusarium sp. FIESC3 the causal agent of seed rot in onion (Allium cepa L.)" in Zbornik Matice srpske za prirodne nauke, no. 132 (2017):9-17,
https://doi.org/10.2298/ZMSPN1732009I . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Gvozdanović-Varga, Jelica
AU  - Tatić, Mladen
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1629
AB  - Garlic (Allium sativum L.) is widely cultivated in Serbia, covering more than 7,000 ha, mostly concentrated in the northern part of country, Vojvodina Province. During 2016, infected garlic bulbs occurred in storage and warehouses in several localities of Vojvodina Province. Garlic bulbs and cloves were softened, spongy, or sunken, and covered with white, light pink, or reddish fungal growth (mycelium). Fifty samples of symptomatic garlic bulbs were taken, surface disinfested in 1% NaOCl for 2 to 3 min, followed by three serial washings of sterilized distilled water and dried under aseptic conditions. Pieces (3 to 4 mm) excised from clove tissue were transferred on potato dextrose agar (PDA) and incubated for 7 days at 25°C in the dark. After 7 days, all isolates were examined morphologically and the fungal isolate was cleaned up by subculturing successively and selected by single-spore isolation. A pathogenicity test was conducted with 21 Fusarium isolates by inoculation of five garilc cloves cv. Bosut, previously sterilized in 0.5% NaOCl for 60 s, rinsed four times in sterilized distilled water, and wounded to a depth of 4 mm (Palmero et al. 2012). The five wounded cloves were inoculated with each fungal isolate and incubated in a growth chamber at 25°C until symptoms of rot appeared. All isolates were reisolated from artificially inoculated garlic cloves, completing Koch’s postulates. Colony morphology was recorded from cultures grown on PDA and carnation leaf agar (CLA) (Castañares et al. 2011; Leslie and Summerell 2006). One isolate, when grown on PDA, rapidly produced abundant, dense, white, aerial mycelium that became pink with age and formed red pigments in the medium. On CLA, macroconidia were abundant, relatively slender, curved to lunate, and three to five septate. Microconidia were abundant, napiform, oval or pyriform, zero to one septate, and commonly clustered in false heads. Chlamydospores were absent. On the basis of fungal morphology, the fungus was identified as Fusarium tricinctum (Corda) Saccardo (Gerlach and Nirenberg 1982). To confirm the morphological identification, total genomic DNA was extracted from the mycelium of the 21 isolates with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2 (Geiser et al. 2004). The sequences were compared with those in GenBank. The TEF sequence (accession no. KX611146) showed 100% similarity with several F. tricinctum sequences (e.g., HM068307, EU744838, and EU744837). Based on the completion of Koch’s postulates and sequence analysis, to our knowledge, this is the first report of F. tricinctum causing pink rot of garlic bulbs in Serbia. This species is known to produce mycotoxins severe for human health and monitoring of storage garlic in Serbia is continued.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia
EP  - 382
IS  - 2
SP  - 382
VL  - 101
DO  - 10.1094/PDIS-09-16-1333-PDN
ER  - 

@article{
author = "Ignjatov, Maja and Milošević, Dragana and Nikolić, Zorica and Gvozdanović-Varga, Jelica and Tatić, Mladen and Popović, Tatjana and Ivanović, Žarko",
year = "2017",
abstract = "Garlic (Allium sativum L.) is widely cultivated in Serbia, covering more than 7,000 ha, mostly concentrated in the northern part of country, Vojvodina Province. During 2016, infected garlic bulbs occurred in storage and warehouses in several localities of Vojvodina Province. Garlic bulbs and cloves were softened, spongy, or sunken, and covered with white, light pink, or reddish fungal growth (mycelium). Fifty samples of symptomatic garlic bulbs were taken, surface disinfested in 1% NaOCl for 2 to 3 min, followed by three serial washings of sterilized distilled water and dried under aseptic conditions. Pieces (3 to 4 mm) excised from clove tissue were transferred on potato dextrose agar (PDA) and incubated for 7 days at 25°C in the dark. After 7 days, all isolates were examined morphologically and the fungal isolate was cleaned up by subculturing successively and selected by single-spore isolation. A pathogenicity test was conducted with 21 Fusarium isolates by inoculation of five garilc cloves cv. Bosut, previously sterilized in 0.5% NaOCl for 60 s, rinsed four times in sterilized distilled water, and wounded to a depth of 4 mm (Palmero et al. 2012). The five wounded cloves were inoculated with each fungal isolate and incubated in a growth chamber at 25°C until symptoms of rot appeared. All isolates were reisolated from artificially inoculated garlic cloves, completing Koch’s postulates. Colony morphology was recorded from cultures grown on PDA and carnation leaf agar (CLA) (Castañares et al. 2011; Leslie and Summerell 2006). One isolate, when grown on PDA, rapidly produced abundant, dense, white, aerial mycelium that became pink with age and formed red pigments in the medium. On CLA, macroconidia were abundant, relatively slender, curved to lunate, and three to five septate. Microconidia were abundant, napiform, oval or pyriform, zero to one septate, and commonly clustered in false heads. Chlamydospores were absent. On the basis of fungal morphology, the fungus was identified as Fusarium tricinctum (Corda) Saccardo (Gerlach and Nirenberg 1982). To confirm the morphological identification, total genomic DNA was extracted from the mycelium of the 21 isolates with a DNeasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2 (Geiser et al. 2004). The sequences were compared with those in GenBank. The TEF sequence (accession no. KX611146) showed 100% similarity with several F. tricinctum sequences (e.g., HM068307, EU744838, and EU744837). Based on the completion of Koch’s postulates and sequence analysis, to our knowledge, this is the first report of F. tricinctum causing pink rot of garlic bulbs in Serbia. This species is known to produce mycotoxins severe for human health and monitoring of storage garlic in Serbia is continued.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia",
pages = "382-382",
number = "2",
volume = "101",
doi = "10.1094/PDIS-09-16-1333-PDN"
}

Ignjatov, M., Milošević, D., Nikolić, Z., Gvozdanović-Varga, J., Tatić, M., Popović, T.,& Ivanović, Ž.. (2017). First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(2), 382-382.
https://doi.org/10.1094/PDIS-09-16-1333-PDN

Ignjatov M, Milošević D, Nikolić Z, Gvozdanović-Varga J, Tatić M, Popović T, Ivanović Ž. First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia. in Plant Disease. 2017;101(2):382-382.
doi:10.1094/PDIS-09-16-1333-PDN .

Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Gvozdanović-Varga, Jelica, Tatić, Mladen, Popović, Tatjana, Ivanović, Žarko, "First Report of Fusarium tricinctum Causing Rot of Garlic Bulbs in Serbia" in Plant Disease, 101, no. 2 (2017):382-382,
https://doi.org/10.1094/PDIS-09-16-1333-PDN . .

TY  - JOUR
AU  - Ivanović, Žarko
AU  - Blagojević, J.
AU  - Popović, Tatjana
AU  - Ignjatov, Maja
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1686
AB  - Paeonia lactiflora Pall. (herbaceous peony), a perennial flower, has been grown worldwide in gardens and landscapes. Peony plants cv. Sarah Bernard with leaf blight were observed in two home gardens located in Jagodina, central Serbia. To our knowledge, this is the first report of Botrytis blight on P. lactiflora in Serbia.
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia
EP  - 1678
IS  - 9
SP  - 1677
VL  - 101
DO  - 10.1094/PDIS-09-16-1327-PDN
ER  - 

@article{
author = "Ivanović, Žarko and Blagojević, J. and Popović, Tatjana and Ignjatov, Maja",
year = "2017",
abstract = "Paeonia lactiflora Pall. (herbaceous peony), a perennial flower, has been grown worldwide in gardens and landscapes. Peony plants cv. Sarah Bernard with leaf blight were observed in two home gardens located in Jagodina, central Serbia. To our knowledge, this is the first report of Botrytis blight on P. lactiflora in Serbia.",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia",
pages = "1678-1677",
number = "9",
volume = "101",
doi = "10.1094/PDIS-09-16-1327-PDN"
}

Ivanović, Ž., Blagojević, J., Popović, T.,& Ignjatov, M.. (2017). First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(9), 1677-1678.
https://doi.org/10.1094/PDIS-09-16-1327-PDN

Ivanović Ž, Blagojević J, Popović T, Ignjatov M. First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia. in Plant Disease. 2017;101(9):1677-1678.
doi:10.1094/PDIS-09-16-1327-PDN .

Ivanović, Žarko, Blagojević, J., Popović, Tatjana, Ignjatov, Maja, "First Report of Botrytis Blight Caused by Botrytis cinerea on Paeonia lactiflora in Serbia" in Plant Disease, 101, no. 9 (2017):1677-1678,
https://doi.org/10.1094/PDIS-09-16-1327-PDN . .

TY  - JOUR
AU  - Blagojević, J.
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
AU  - Ignjatov, Maja
AU  - Vukojević, J.
PY  - 2017
UR  - http://fiver.ifvcns.rs/handle/123456789/1630
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia
EP  - 385
IS  - 2
SP  - 384
VL  - 101
DO  - 10.1094/PDIS-08-16-1106-PDN
ER  - 

@article{
author = "Blagojević, J. and Ivanović, Žarko and Popović, Tatjana and Ignjatov, Maja and Vukojević, J.",
year = "2017",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia",
pages = "385-384",
number = "2",
volume = "101",
doi = "10.1094/PDIS-08-16-1106-PDN"
}

Blagojević, J., Ivanović, Ž., Popović, T., Ignjatov, M.,& Vukojević, J.. (2017). First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 101(2), 384-385.
https://doi.org/10.1094/PDIS-08-16-1106-PDN

Blagojević J, Ivanović Ž, Popović T, Ignjatov M, Vukojević J. First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia. in Plant Disease. 2017;101(2):384-385.
doi:10.1094/PDIS-08-16-1106-PDN .

Blagojević, J., Ivanović, Žarko, Popović, Tatjana, Ignjatov, Maja, Vukojević, J., "First Report of Leaf Spot Disease on Spathiphyllum sp Caused by Alternaria alternata in Serbia" in Plant Disease, 101, no. 2 (2017):384-385,
https://doi.org/10.1094/PDIS-08-16-1106-PDN . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Janjatović, Sanja
AU  - Ignjatov, Maja
AU  - Milovanović, Predrag
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1534
AB  - Brown rot or bacterial wilt caused by bacterium Ralstonia solanacearum is the main limiting factor in potato production. Quarantine measures are necessary to avoid spread of disease to disease-free areas. R. solanacearum has been shown to contaminate watercourses from which crop irrigation is then prohibited causing further potential losses in yield and quality. The bacteria also spread via surfaces that diseased seed potatoes come into contact with. This study showed bactericidal activity of chlorine dioxide (CIO2) on R. solanacearum for disinfection of water, surface and equipment. The results showed that CIO2 solution at concentration of 2 ppm at 30 minutes of exposure time had bactericidal effect for disinfection of water. For surface and equipment disinfection, concentration of 50 ppm showed total efficacy at 30 min and 5 sec exposure time, respectively. Results suggest that use of CIO2 as a disinfectant has a potential for control of brown rot pathogen in water, storage and equipment.
AB  - Mrka trulež ili bakteriozna uvelost krompira prozrokovana bakterijom Ralstonia solanacearum ograničavajući je faktor uspešne proizvodnje krompira. Sprovođenje karantinskih mera zaštite je neophodno kako bi se izbeglo širenje bakterije u regione u kojima bolest nije prisutna. S obzirom da R. solanacearum može kontaminirati vodene tokove i izvore koji služe za navodnjavanje useva, zabrana korišćenja dovodi do dodatnih potencijalnih gubitaka u prinosu i kvalitetu krompira. Bakterija takođe kontaminira površine sa kojima zaraženi semenski krompir dolazi u dodir. U ovom radu prikazana je baktericidna aktivnost hlor-dioksida (CIO2) na bakteriju R. solanacearum u cilju dezinfekcije vode, površine i opreme. Dobijeni rezultati pokazuju da CIO2 ispoljava baktericidni efekat za dezinfekciju vode u koncentraciji od 2 ppm nakon 30 minuta ekspozicije, za dezinfekciju površine u koncentraciji 50 ppm nakon 30 minuta ekspozicije i za dezinfekciju opreme u koncentraciji 50 ppm nakon 5 sekundi ekspozicije. Rezultati dobijeni u ovom radu ukazuju da CIO2 poseduje potencijal kao dezinficijens za kontrolu prouzrokovača mrke truleži krompira u vodi, skladištu i opremi.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment
T1  - Hlor-dioksid kao dezinficijens za kontrolu Ralstonia solanacearum u vodi, skladištu i opremi
EP  - 84
IS  - 2
SP  - 81
VL  - 53
DO  - 10.5937/ratpov53-9982
ER  - 

@article{
author = "Popović, Tatjana and Ivanović, Žarko and Janjatović, Sanja and Ignjatov, Maja and Milovanović, Predrag",
year = "2016",
abstract = "Brown rot or bacterial wilt caused by bacterium Ralstonia solanacearum is the main limiting factor in potato production. Quarantine measures are necessary to avoid spread of disease to disease-free areas. R. solanacearum has been shown to contaminate watercourses from which crop irrigation is then prohibited causing further potential losses in yield and quality. The bacteria also spread via surfaces that diseased seed potatoes come into contact with. This study showed bactericidal activity of chlorine dioxide (CIO2) on R. solanacearum for disinfection of water, surface and equipment. The results showed that CIO2 solution at concentration of 2 ppm at 30 minutes of exposure time had bactericidal effect for disinfection of water. For surface and equipment disinfection, concentration of 50 ppm showed total efficacy at 30 min and 5 sec exposure time, respectively. Results suggest that use of CIO2 as a disinfectant has a potential for control of brown rot pathogen in water, storage and equipment., Mrka trulež ili bakteriozna uvelost krompira prozrokovana bakterijom Ralstonia solanacearum ograničavajući je faktor uspešne proizvodnje krompira. Sprovođenje karantinskih mera zaštite je neophodno kako bi se izbeglo širenje bakterije u regione u kojima bolest nije prisutna. S obzirom da R. solanacearum može kontaminirati vodene tokove i izvore koji služe za navodnjavanje useva, zabrana korišćenja dovodi do dodatnih potencijalnih gubitaka u prinosu i kvalitetu krompira. Bakterija takođe kontaminira površine sa kojima zaraženi semenski krompir dolazi u dodir. U ovom radu prikazana je baktericidna aktivnost hlor-dioksida (CIO2) na bakteriju R. solanacearum u cilju dezinfekcije vode, površine i opreme. Dobijeni rezultati pokazuju da CIO2 ispoljava baktericidni efekat za dezinfekciju vode u koncentraciji od 2 ppm nakon 30 minuta ekspozicije, za dezinfekciju površine u koncentraciji 50 ppm nakon 30 minuta ekspozicije i za dezinfekciju opreme u koncentraciji 50 ppm nakon 5 sekundi ekspozicije. Rezultati dobijeni u ovom radu ukazuju da CIO2 poseduje potencijal kao dezinficijens za kontrolu prouzrokovača mrke truleži krompira u vodi, skladištu i opremi.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment, Hlor-dioksid kao dezinficijens za kontrolu Ralstonia solanacearum u vodi, skladištu i opremi",
pages = "84-81",
number = "2",
volume = "53",
doi = "10.5937/ratpov53-9982"
}

Popović, T., Ivanović, Ž., Janjatović, S., Ignjatov, M.,& Milovanović, P.. (2016). Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 53(2), 81-84.
https://doi.org/10.5937/ratpov53-9982

Popović T, Ivanović Ž, Janjatović S, Ignjatov M, Milovanović P. Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2016;53(2):81-84.
doi:10.5937/ratpov53-9982 .

Popović, Tatjana, Ivanović, Žarko, Janjatović, Sanja, Ignjatov, Maja, Milovanović, Predrag, "Chlorine dioxide as a disinfectant for Ralstonia solanacearum control in water, storage and equipment" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 53, no. 2 (2016):81-84,
https://doi.org/10.5937/ratpov53-9982 . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Popović, Tatjana
AU  - Milošević, Dragana
AU  - Vasić, Mirjana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Ivanović, Žarko
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1518
AB  - During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot.
AB  - Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.
PB  - Institut za ratarstvo i povrtarstvo, Novi Sad
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia
T1  - Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji
EP  - 45
IS  - 2
SP  - 42
VL  - 53
DO  - 10.5937/ratpov53-9984
ER  - 

@article{
author = "Ignjatov, Maja and Popović, Tatjana and Milošević, Dragana and Vasić, Mirjana and Nikolić, Zorica and Tamindžić, Gordana and Ivanović, Žarko",
year = "2016",
abstract = "During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot., Tokom rutinske kontrole kvaliteta semena pasulja (Phaseolus vulgaris L.) u 2015. godini ustanovljeno je prisustvo Fusarium sp. u visokom procentu, u proseku 17%. Cilj ovog rada bio je izolacija i identifikacija prouzrokovača truleži semena pasulja, na osnovu morfoloških i molekularnih karakteristika patogena. Odgajivačke karakteristike 14 odabranih izolata ocenenjene su na PDA i CLA podlogama. Ekstrakcija DNK 14 odabranih izolata obavljena je direktno iz 100 mg sveže micelije, korišćenjem Dneasy Plant Mini Kit (Qiagen, Hilden, Nemačka). Amplifikacija DNK obavljena je pomoću PCR korišćenjem para prajmera EF1 i EF2. U svim proučavanim izolatima formirani su amplikoni veličine 700 bp. Identifikacija jednog odabranog izolata izvršena je sekvenciranjem translacionog faktora EF-1 a gena. Na osnovu dobijenih rezultata ustanovljeno je da je prouzrokovač truleži semena pasulja Fusarium proliferatum.",
publisher = "Institut za ratarstvo i povrtarstvo, Novi Sad",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia, Pojava, identifikacija i filogenetska analiza Fusarium proliferatum prouzrokovača truleži semena pasulja (Phaseolus vulgaris L.) u Srbiji",
pages = "45-42",
number = "2",
volume = "53",
doi = "10.5937/ratpov53-9984"
}

Ignjatov, M., Popović, T., Milošević, D., Vasić, M., Nikolić, Z., Tamindžić, G.,& Ivanović, Ž.. (2016). Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Institut za ratarstvo i povrtarstvo, Novi Sad., 53(2), 42-45.
https://doi.org/10.5937/ratpov53-9984

Ignjatov M, Popović T, Milošević D, Vasić M, Nikolić Z, Tamindžić G, Ivanović Ž. Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2016;53(2):42-45.
doi:10.5937/ratpov53-9984 .

Ignjatov, Maja, Popović, Tatjana, Milošević, Dragana, Vasić, Mirjana, Nikolić, Zorica, Tamindžić, Gordana, Ivanović, Žarko, "Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 53, no. 2 (2016):42-45,
https://doi.org/10.5937/ratpov53-9984 . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Ignjatov, Maja
AU  - Milošević, Drago M.
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1447
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pseudomonas syringae pv. coriandricola Causing Bacterial Leaf Spot on Carrot, Parsley, and Parsnip in Serbia
EP  - 417
IS  - 3
SP  - 416
VL  - 99
DO  - 10.1094/PDIS-10-14-1041-PDN
ER  - 

@article{
author = "Popović, Tatjana and Ivanović, Žarko and Ignjatov, Maja and Milošević, Drago M.",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pseudomonas syringae pv. coriandricola Causing Bacterial Leaf Spot on Carrot, Parsley, and Parsnip in Serbia",
pages = "417-416",
number = "3",
volume = "99",
doi = "10.1094/PDIS-10-14-1041-PDN"
}

Popović, T., Ivanović, Ž., Ignjatov, M.,& Milošević, D. M.. (2015). First Report of Pseudomonas syringae pv. coriandricola Causing Bacterial Leaf Spot on Carrot, Parsley, and Parsnip in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(3), 416-417.
https://doi.org/10.1094/PDIS-10-14-1041-PDN

Popović T, Ivanović Ž, Ignjatov M, Milošević DM. First Report of Pseudomonas syringae pv. coriandricola Causing Bacterial Leaf Spot on Carrot, Parsley, and Parsnip in Serbia. in Plant Disease. 2015;99(3):416-417.
doi:10.1094/PDIS-10-14-1041-PDN .

Popović, Tatjana, Ivanović, Žarko, Ignjatov, Maja, Milošević, Drago M., "First Report of Pseudomonas syringae pv. coriandricola Causing Bacterial Leaf Spot on Carrot, Parsley, and Parsnip in Serbia" in Plant Disease, 99, no. 3 (2015):416-417,
https://doi.org/10.1094/PDIS-10-14-1041-PDN . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Ignjatov, Maja
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1459
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pseudomonas viridiflava Causing Pith Necrosis of Tomato (Solanum lycopersicum) in Serbia
EP  - 1033
IS  - 7
SP  - 1033
VL  - 99
DO  - 10.1094/PDIS-01-15-0052-PDN
ER  - 

@article{
author = "Popović, Tatjana and Ivanović, Žarko and Ignjatov, Maja",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pseudomonas viridiflava Causing Pith Necrosis of Tomato (Solanum lycopersicum) in Serbia",
pages = "1033-1033",
number = "7",
volume = "99",
doi = "10.1094/PDIS-01-15-0052-PDN"
}

Popović, T., Ivanović, Ž.,& Ignjatov, M.. (2015). First Report of Pseudomonas viridiflava Causing Pith Necrosis of Tomato (Solanum lycopersicum) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(7), 1033-1033.
https://doi.org/10.1094/PDIS-01-15-0052-PDN

Popović T, Ivanović Ž, Ignjatov M. First Report of Pseudomonas viridiflava Causing Pith Necrosis of Tomato (Solanum lycopersicum) in Serbia. in Plant Disease. 2015;99(7):1033-1033.
doi:10.1094/PDIS-01-15-0052-PDN .

Popović, Tatjana, Ivanović, Žarko, Ignjatov, Maja, "First Report of Pseudomonas viridiflava Causing Pith Necrosis of Tomato (Solanum lycopersicum) in Serbia" in Plant Disease, 99, no. 7 (2015):1033-1033,
https://doi.org/10.1094/PDIS-01-15-0052-PDN . .

TY  - JOUR
AU  - Blagojević, J.
AU  - Ivanović, Žarko
AU  - Oro, V.
AU  - Dolovac, N.
AU  - Popović, Tatjana
AU  - Ignjatov, Maja
AU  - Vukojević, J.
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1468
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia
EP  - 730
IS  - 5
SP  - 730
VL  - 99
DO  - 10.1094/PDIS-11-14-1181-PDN
ER  - 

@article{
author = "Blagojević, J. and Ivanović, Žarko and Oro, V. and Dolovac, N. and Popović, Tatjana and Ignjatov, Maja and Vukojević, J.",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia",
pages = "730-730",
number = "5",
volume = "99",
doi = "10.1094/PDIS-11-14-1181-PDN"
}

Blagojević, J., Ivanović, Ž., Oro, V., Dolovac, N., Popović, T., Ignjatov, M.,& Vukojević, J.. (2015). First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(5), 730-730.
https://doi.org/10.1094/PDIS-11-14-1181-PDN

Blagojević J, Ivanović Ž, Oro V, Dolovac N, Popović T, Ignjatov M, Vukojević J. First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia. in Plant Disease. 2015;99(5):730-730.
doi:10.1094/PDIS-11-14-1181-PDN .

Blagojević, J., Ivanović, Žarko, Oro, V., Dolovac, N., Popović, Tatjana, Ignjatov, Maja, Vukojević, J., "First Report of Horseradish Leaf Spot Caused by Alternaria brassicae in Serbia" in Plant Disease, 99, no. 5 (2015):730-730,
https://doi.org/10.1094/PDIS-11-14-1181-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Gvozdanović-Varga, Jelica
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1458
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia
EP  - 723
IS  - 5
SP  - 723
VL  - 99
DO  - 10.1094/PDIS-10-14-1097-PDN
ER  - 

@article{
author = "Ignjatov, Maja and Gvozdanović-Varga, Jelica and Milošević, Dragana and Nikolić, Zorica and Ivanović, Žarko and Popović, Tatjana",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia",
pages = "723-723",
number = "5",
volume = "99",
doi = "10.1094/PDIS-10-14-1097-PDN"
}

Ignjatov, M., Gvozdanović-Varga, J., Milošević, D., Nikolić, Z., Ivanović, Ž.,& Popović, T.. (2015). First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(5), 723-723.
https://doi.org/10.1094/PDIS-10-14-1097-PDN

Ignjatov M, Gvozdanović-Varga J, Milošević D, Nikolić Z, Ivanović Ž, Popović T. First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia. in Plant Disease. 2015;99(5):723-723.
doi:10.1094/PDIS-10-14-1097-PDN .

Ignjatov, Maja, Gvozdanović-Varga, Jelica, Milošević, Dragana, Nikolić, Zorica, Ivanović, Žarko, Popović, Tatjana, "First Report of Bacterial Leaf Spot of Chard (Beta vulgaris subsp cicla) Caused by Pseudomonas syringae pv. syringae in Serbia" in Plant Disease, 99, no. 5 (2015):723-723,
https://doi.org/10.1094/PDIS-10-14-1097-PDN . .

TY  - JOUR
AU  - Ignjatov, Maja
AU  - Milošević, Dragana
AU  - Nikolić, Zorica
AU  - Tamindžić, Gordana
AU  - Gvozdanović-Varga, Jelica
AU  - Ivanović, Žarko
AU  - Popović, Tatjana
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1472
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia
EP  - 1278
IS  - 9
SP  - 1277
VL  - 99
DO  - 10.1094/PDIS-01-15-0082-PDN
ER  - 

@article{
author = "Ignjatov, Maja and Milošević, Dragana and Nikolić, Zorica and Tamindžić, Gordana and Gvozdanović-Varga, Jelica and Ivanović, Žarko and Popović, Tatjana",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia",
pages = "1278-1277",
number = "9",
volume = "99",
doi = "10.1094/PDIS-01-15-0082-PDN"
}

Ignjatov, M., Milošević, D., Nikolić, Z., Tamindžić, G., Gvozdanović-Varga, J., Ivanović, Ž.,& Popović, T.. (2015). First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(9), 1277-1278.
https://doi.org/10.1094/PDIS-01-15-0082-PDN

Ignjatov M, Milošević D, Nikolić Z, Tamindžić G, Gvozdanović-Varga J, Ivanović Ž, Popović T. First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia. in Plant Disease. 2015;99(9):1277-1278.
doi:10.1094/PDIS-01-15-0082-PDN .

Ignjatov, Maja, Milošević, Dragana, Nikolić, Zorica, Tamindžić, Gordana, Gvozdanović-Varga, Jelica, Ivanović, Žarko, Popović, Tatjana, "First Report of Fusarium sp FIESC 3 on Onion Seed in Serbia" in Plant Disease, 99, no. 9 (2015):1277-1278,
https://doi.org/10.1094/PDIS-01-15-0082-PDN . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Ivanović, Žarko
AU  - Trkulja, Nenad
AU  - Milosavljević, A.
AU  - Ignjatov, Maja
PY  - 2015
UR  - http://fiver.ifvcns.rs/handle/123456789/1469
PB  - Amer Phytopathological Soc, St Paul
T2  - Plant Disease
T1  - First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia
EP  - 724
IS  - 5
SP  - 724
VL  - 99
DO  - 10.1094/PDIS-11-14-1212-PDN
ER  - 

@article{
author = "Popović, Tatjana and Ivanović, Žarko and Trkulja, Nenad and Milosavljević, A. and Ignjatov, Maja",
year = "2015",
publisher = "Amer Phytopathological Soc, St Paul",
journal = "Plant Disease",
title = "First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia",
pages = "724-724",
number = "5",
volume = "99",
doi = "10.1094/PDIS-11-14-1212-PDN"
}

Popović, T., Ivanović, Ž., Trkulja, N., Milosavljević, A.,& Ignjatov, M.. (2015). First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia. in Plant Disease
Amer Phytopathological Soc, St Paul., 99(5), 724-724.
https://doi.org/10.1094/PDIS-11-14-1212-PDN

Popović T, Ivanović Ž, Trkulja N, Milosavljević A, Ignjatov M. First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia. in Plant Disease. 2015;99(5):724-724.
doi:10.1094/PDIS-11-14-1212-PDN .

Popović, Tatjana, Ivanović, Žarko, Trkulja, Nenad, Milosavljević, A., Ignjatov, Maja, "First Report of Pseudomonas syringae pv. syringae on Pea (Pisum sativum) in Serbia" in Plant Disease, 99, no. 5 (2015):724-724,
https://doi.org/10.1094/PDIS-11-14-1212-PDN . .

TY  - JOUR
AU  - Popović, Tatjana
AU  - Balaž, Jelica
AU  - Ignjatov, Maja
AU  - Mitrović, Petar
AU  - Gavrilović, Veljko
AU  - Jošić, Dragana
PY  - 2014
UR  - http://fiver.ifvcns.rs/handle/123456789/1397
AB  - During 2010, black rot symptoms were observed in oilseed rape plants grown in a commercial plot in Serbia. Ten bacterial isolates obtained from diseased plants, and identified as Xanthomonas campestris pv. campestris (Xcc) based on pathogenicity, physiological and biochemical tests, PTA-ELISA and 16S rDNA sequences analysis, were investigated in detail. Strains were characterized by comparing them by rep-PCR fingerprints using ERIC and (GTG)(5) primers. The 16S rDNA sequences of strains TUr1 and TUr6 were deposited in GenBank under accession Nos. KF057196 and KF057197, respectively. Phylogenetic analysis of the 16S regions showed high similarity level for oilseed rape representative strains and Xcc strains of different origin isolated from kale, cabbage and broccoli.
PB  - Edizioni Ets
T2  - Journal of Plant Pathology
T1  - Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in Serbia
EP  - 560
IS  - 3
SP  - 553
VL  - 96
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1397
ER  - 

@article{
author = "Popović, Tatjana and Balaž, Jelica and Ignjatov, Maja and Mitrović, Petar and Gavrilović, Veljko and Jošić, Dragana",
year = "2014",
abstract = "During 2010, black rot symptoms were observed in oilseed rape plants grown in a commercial plot in Serbia. Ten bacterial isolates obtained from diseased plants, and identified as Xanthomonas campestris pv. campestris (Xcc) based on pathogenicity, physiological and biochemical tests, PTA-ELISA and 16S rDNA sequences analysis, were investigated in detail. Strains were characterized by comparing them by rep-PCR fingerprints using ERIC and (GTG)(5) primers. The 16S rDNA sequences of strains TUr1 and TUr6 were deposited in GenBank under accession Nos. KF057196 and KF057197, respectively. Phylogenetic analysis of the 16S regions showed high similarity level for oilseed rape representative strains and Xcc strains of different origin isolated from kale, cabbage and broccoli.",
publisher = "Edizioni Ets",
journal = "Journal of Plant Pathology",
title = "Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in Serbia",
pages = "560-553",
number = "3",
volume = "96",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1397"
}

Popović, T., Balaž, J., Ignjatov, M., Mitrović, P., Gavrilović, V.,& Jošić, D.. (2014). Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in Serbia. in Journal of Plant Pathology
Edizioni Ets., 96(3), 553-560.
https://hdl.handle.net/21.15107/rcub_fiver_1397

Popović T, Balaž J, Ignjatov M, Mitrović P, Gavrilović V, Jošić D. Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in Serbia. in Journal of Plant Pathology. 2014;96(3):553-560.
https://hdl.handle.net/21.15107/rcub_fiver_1397 .

Popović, Tatjana, Balaž, Jelica, Ignjatov, Maja, Mitrović, Petar, Gavrilović, Veljko, Jošić, Dragana, "Identification and genetic characterisation of xanthomonas campestris pv. Campestris as an oilseed rape pathogen in Serbia" in Journal of Plant Pathology, 96, no. 3 (2014):553-560,
https://hdl.handle.net/21.15107/rcub_fiver_1397 .