TY  - JOUR
AU  - Radovanović, Katarina
AU  - Gavarić, Neda
AU  - Švarc-Gajić, Jaroslava
AU  - Brezo-Borjan, Tanja
AU  - Zlatković, Bojan
AU  - Lončar, Biljana
AU  - Aćimović, Milica
PY  - 2023
UR  - http://fiver.ifvcns.rs/handle/123456789/3342
AB  - The genus Achillea has significant medical potential due to the presence of highly bioactive compounds in its chemical composition. To take advantage of plants’ biomedical potential, it is of great importance to use a proper extraction process. This study aimed to determine and compare the preliminary chemical composition of five different Achillea species extracted with two conventional (infusion and maceration) and two non-conventional (ultrasound-assisted extraction (UAE) and Subcritical water extraction (SWE)) techniques. The extracts were prepared using the previously described procedures for infusion, maceration, UAE and SWE extraction. For all extracts, the extraction yield (dry extract (DE)) was determined. The analyzed extracts were preliminarily chemically characterized spectrophotometrically in terms of total phenolic content (TPC) and total flavonoid content (TFC). The obtained results showed that non-conventional techniques delivered higher values of TPC and TFC than conventional. There is a statistically significant increase in DE and TPC content when applying SWE for all observed Achillea species. The highest DE value, 48.80 ± 1.76%, was observed for A. asplenifolia. The highest TPC values were observed after applying SWE: 93.63 ± 1.01 mg GAE/g DE for A. millefolium, and 90.12 ± 0.87 mg GAE/g DE for A. crithmifolia. The results for TFC revealed a statistically significant difference in values, with A. nobilis subsp. nelreichii as the sample with the highest content of TFC (11.11 ± 0.22 mg QE/g DE) when using UAE. Consequently, it could be concluded that SWE is a superior non-conventional extraction technique, and A. nobilis subsp. nerleichii presents as the most promising plant.
PB  - Basel : MDPI
T2  - Processes - Basel
T1  - Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species
IS  - 1
SP  - 86
VL  - 11
DO  - 10.3390/pr11010086
ER  - 

@article{
author = "Radovanović, Katarina and Gavarić, Neda and Švarc-Gajić, Jaroslava and Brezo-Borjan, Tanja and Zlatković, Bojan and Lončar, Biljana and Aćimović, Milica",
year = "2023",
abstract = "The genus Achillea has significant medical potential due to the presence of highly bioactive compounds in its chemical composition. To take advantage of plants’ biomedical potential, it is of great importance to use a proper extraction process. This study aimed to determine and compare the preliminary chemical composition of five different Achillea species extracted with two conventional (infusion and maceration) and two non-conventional (ultrasound-assisted extraction (UAE) and Subcritical water extraction (SWE)) techniques. The extracts were prepared using the previously described procedures for infusion, maceration, UAE and SWE extraction. For all extracts, the extraction yield (dry extract (DE)) was determined. The analyzed extracts were preliminarily chemically characterized spectrophotometrically in terms of total phenolic content (TPC) and total flavonoid content (TFC). The obtained results showed that non-conventional techniques delivered higher values of TPC and TFC than conventional. There is a statistically significant increase in DE and TPC content when applying SWE for all observed Achillea species. The highest DE value, 48.80 ± 1.76%, was observed for A. asplenifolia. The highest TPC values were observed after applying SWE: 93.63 ± 1.01 mg GAE/g DE for A. millefolium, and 90.12 ± 0.87 mg GAE/g DE for A. crithmifolia. The results for TFC revealed a statistically significant difference in values, with A. nobilis subsp. nelreichii as the sample with the highest content of TFC (11.11 ± 0.22 mg QE/g DE) when using UAE. Consequently, it could be concluded that SWE is a superior non-conventional extraction technique, and A. nobilis subsp. nerleichii presents as the most promising plant.",
publisher = "Basel : MDPI",
journal = "Processes - Basel",
title = "Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species",
number = "1",
pages = "86",
volume = "11",
doi = "10.3390/pr11010086"
}

Radovanović, K., Gavarić, N., Švarc-Gajić, J., Brezo-Borjan, T., Zlatković, B., Lončar, B.,& Aćimović, M.. (2023). Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species. in Processes - Basel
Basel : MDPI., 11(1), 86.
https://doi.org/10.3390/pr11010086

Radovanović K, Gavarić N, Švarc-Gajić J, Brezo-Borjan T, Zlatković B, Lončar B, Aćimović M. Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species. in Processes - Basel. 2023;11(1):86.
doi:10.3390/pr11010086 .

Radovanović, Katarina, Gavarić, Neda, Švarc-Gajić, Jaroslava, Brezo-Borjan, Tanja, Zlatković, Bojan, Lončar, Biljana, Aćimović, Milica, "Subcritical Water Extraction as an Effective Technique for the Isolation of Phenolic Compounds of Achillea Species" in Processes - Basel, 11, no. 1 (2023):86,
https://doi.org/10.3390/pr11010086 . .

TY  - JOUR
AU  - Aćimović, Milica
AU  - Šeregelj, Vanja
AU  - Šovljanski, Olja
AU  - Tumbas Šaponjac, Vesna
AU  - Švarc Gajić, Jaroslava
AU  - Brezo-Borjan, Tanja
AU  - Pezo, Lato
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2196
AB  - As the main goal of this study was the verification of Satureja kitaibelii Wierzb. ex Heuff. in form of the crude extract obtained by subcritical water extraction (SWE) technique in terms of polyphenolic composition, in vitro antioxidant capacity, antihyperglycemic, and anti-inflammatory activities as well as an antimicrobial effect against 10 microorganisms. HPLC analysis showed the presence of ten compounds in a total concentration of 89.33 mg/g of the SWE extract, and demonstrated that subcritical water polarity was the most selective for extracting syringic and caffeic acid, as well as epicatechin (37.88 18.06, and 10.04 mg/g, respectively), while flavonoids like rutin, luteolin, and apigenin were present in low concentration (<2 mg/g). Results of antioxidant activity assays revealed that superoxide anion and ABTS•+ radical models expressed the highest scavenging abilities (437.35 and 412.09 μmol TE/g, respectively), followed by lipid radicals (69.61 μmol TE/g), and DPPH• (32.28 μmol TE/g), while in the case of ferric ion reducing ability was noted a high value (118.19 μmol TE/g). S. kitaibelii SWE was less efficient in the antihyperglycemic assay (AhgA = 30.72 %), but the high antiinflammatory activity of S. kitaibelii SWE was observed (30.35 %). The antimicrobial activity was demonstrated against Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes as well as Saccharomyces cerevisiae, Candida albicans, and Aspergillus brasiliensis through antimicrobial screening, minimal inhibitory concentration, and pharmacodynamics study.
PB  - Elsevier
T2  - Industrial Crops and Products
T1  - In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract
SP  - 113672
VL  - 169
DO  - 10.1016/j.indcrop.2021.113672
ER  - 

@article{
author = "Aćimović, Milica and Šeregelj, Vanja and Šovljanski, Olja and Tumbas Šaponjac, Vesna and Švarc Gajić, Jaroslava and Brezo-Borjan, Tanja and Pezo, Lato",
year = "2021",
abstract = "As the main goal of this study was the verification of Satureja kitaibelii Wierzb. ex Heuff. in form of the crude extract obtained by subcritical water extraction (SWE) technique in terms of polyphenolic composition, in vitro antioxidant capacity, antihyperglycemic, and anti-inflammatory activities as well as an antimicrobial effect against 10 microorganisms. HPLC analysis showed the presence of ten compounds in a total concentration of 89.33 mg/g of the SWE extract, and demonstrated that subcritical water polarity was the most selective for extracting syringic and caffeic acid, as well as epicatechin (37.88 18.06, and 10.04 mg/g, respectively), while flavonoids like rutin, luteolin, and apigenin were present in low concentration (<2 mg/g). Results of antioxidant activity assays revealed that superoxide anion and ABTS•+ radical models expressed the highest scavenging abilities (437.35 and 412.09 μmol TE/g, respectively), followed by lipid radicals (69.61 μmol TE/g), and DPPH• (32.28 μmol TE/g), while in the case of ferric ion reducing ability was noted a high value (118.19 μmol TE/g). S. kitaibelii SWE was less efficient in the antihyperglycemic assay (AhgA = 30.72 %), but the high antiinflammatory activity of S. kitaibelii SWE was observed (30.35 %). The antimicrobial activity was demonstrated against Bacillus cereus, Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes as well as Saccharomyces cerevisiae, Candida albicans, and Aspergillus brasiliensis through antimicrobial screening, minimal inhibitory concentration, and pharmacodynamics study.",
publisher = "Elsevier",
journal = "Industrial Crops and Products",
title = "In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract",
pages = "113672",
volume = "169",
doi = "10.1016/j.indcrop.2021.113672"
}

Aćimović, M., Šeregelj, V., Šovljanski, O., Tumbas Šaponjac, V., Švarc Gajić, J., Brezo-Borjan, T.,& Pezo, L.. (2021). In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract. in Industrial Crops and Products
Elsevier., 169, 113672.
https://doi.org/10.1016/j.indcrop.2021.113672

Aćimović M, Šeregelj V, Šovljanski O, Tumbas Šaponjac V, Švarc Gajić J, Brezo-Borjan T, Pezo L. In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract. in Industrial Crops and Products. 2021;169:113672.
doi:10.1016/j.indcrop.2021.113672 .

Aćimović, Milica, Šeregelj, Vanja, Šovljanski, Olja, Tumbas Šaponjac, Vesna, Švarc Gajić, Jaroslava, Brezo-Borjan, Tanja, Pezo, Lato, "In vitro antioxidant, antihyperglycemic, anti-inflammatory, and antimicrobial activity of Satureja kitaibelii Wierzb. ex Heuff. subcritical water extract" in Industrial Crops and Products, 169 (2021):113672,
https://doi.org/10.1016/j.indcrop.2021.113672 . .