TY  - JOUR
AU  - Nagl, Nevena
AU  - Taški-Ajduković, Ksenija
AU  - Popović, Andrea
AU  - Ćurčić, Živko
AU  - Danojević, Dario
AU  - Kovačev, Lazar
PY  - 2011
UR  - http://fiver.ifvcns.rs/handle/123456789/969
AB  - In marker assisted breeding programs, determination of genome polymorphism and development of suitable molecular markers is of the greatest importance. The aim of this research was development of RAPD markers, which will enable quick and cost efficient DNA polymorphism analysis among closely related sugar beet genotypes. The research was conducted on twelve sugar beet genotypes from population of closely related genotypes. Reactions with eight RAPD primers and five primer mixtures resulted in stable and reproducible bands in all samples, with 44 polymorphic and 14 monomorphic loci, and average of 6.13 bands per primer. In two-primer reactions nine new polymorphic bands were detected. Polymorphism information content (PIC) for each primer was calculated, while genetic variation was estimated by calculation of the number of polymorphic loci and their percentage, observed number of alleles, effective number of alleles, and Nei's gene diversity. An unweighted pair group arithmetic mean method (UPGMA) cluster analysis showed that samples were divided in two groups with relatively high coefficient of similarity. The presented results showed that RAPD markers can be suitable for genetic diversity analysis in breeding material with high levels of homology and homozygosity.
AB  - U programima oplemenjivanja uz pomoć molekularnih markera, određivanje polimorfizma genoma i razvoj odgovarajućih markerskih sistema je od najveće važnosti. Cilj ovog rada je bio razvoj RAPD marker sitema sa ciljem njegove primene u brzoj i jeftinog analizi DNK polimorfizma u populacijama srodnih genotipova šećerne repe. Predstavljena istraživanja su sprovedena na dvanaest srodnih genotipova šećerne repe. Kao rezultat reakcija sa osam RAPD prajmera i pet mešavina prajmera dobijeni su stabilni i ponovljivi produkti reakcija kod svih genotipova, sa 44 polimorfna i 14 monomorfnih lokusa, odnosno prosečno 6.13 traka po prajmeru. U reakcijama sa dva prajmera detektovano je devet novih polimorfnih traka. Informativnost prajmera i prajmerskih mešavina je određena pomoću PIC (polymorphism information content) vrednosti, dok je genetska varijabilnosti procenjena određivanjem broja polimorfnih lokusa i njihovim procentom, uočenim brojem alela, efektivnim brojem alela, i genetskog diverziteta po Nei -u. UPGMA (unweighted pair group arithmetic mean method) klaster analiza je pokazala da su uzorci bili grupisani u dve glaven podgrupe sa značajnim koeficijentom sličnosti unutar njih. Predstavljeni rezultati pokazuju da RAPD markeri mogu omogućiti analizu genetske divergentnosti u oplemenjivakom materijalu kod koga se očekuje povećana homologija i stepen homozigotnosti.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika-Belgrade
T1  - Estimation of genetic variation among related sugar beet genotypes by using RAPD
T1  - Ocena genetske varijabilnosti između srodnih genotipova šećerne repe pomoću RAPD markera
EP  - 582
IS  - 3
SP  - 575
VL  - 43
DO  - 10.2298/GENSR1103575N
ER  - 

@article{
author = "Nagl, Nevena and Taški-Ajduković, Ksenija and Popović, Andrea and Ćurčić, Živko and Danojević, Dario and Kovačev, Lazar",
year = "2011",
abstract = "In marker assisted breeding programs, determination of genome polymorphism and development of suitable molecular markers is of the greatest importance. The aim of this research was development of RAPD markers, which will enable quick and cost efficient DNA polymorphism analysis among closely related sugar beet genotypes. The research was conducted on twelve sugar beet genotypes from population of closely related genotypes. Reactions with eight RAPD primers and five primer mixtures resulted in stable and reproducible bands in all samples, with 44 polymorphic and 14 monomorphic loci, and average of 6.13 bands per primer. In two-primer reactions nine new polymorphic bands were detected. Polymorphism information content (PIC) for each primer was calculated, while genetic variation was estimated by calculation of the number of polymorphic loci and their percentage, observed number of alleles, effective number of alleles, and Nei's gene diversity. An unweighted pair group arithmetic mean method (UPGMA) cluster analysis showed that samples were divided in two groups with relatively high coefficient of similarity. The presented results showed that RAPD markers can be suitable for genetic diversity analysis in breeding material with high levels of homology and homozygosity., U programima oplemenjivanja uz pomoć molekularnih markera, određivanje polimorfizma genoma i razvoj odgovarajućih markerskih sistema je od najveće važnosti. Cilj ovog rada je bio razvoj RAPD marker sitema sa ciljem njegove primene u brzoj i jeftinog analizi DNK polimorfizma u populacijama srodnih genotipova šećerne repe. Predstavljena istraživanja su sprovedena na dvanaest srodnih genotipova šećerne repe. Kao rezultat reakcija sa osam RAPD prajmera i pet mešavina prajmera dobijeni su stabilni i ponovljivi produkti reakcija kod svih genotipova, sa 44 polimorfna i 14 monomorfnih lokusa, odnosno prosečno 6.13 traka po prajmeru. U reakcijama sa dva prajmera detektovano je devet novih polimorfnih traka. Informativnost prajmera i prajmerskih mešavina je određena pomoću PIC (polymorphism information content) vrednosti, dok je genetska varijabilnosti procenjena određivanjem broja polimorfnih lokusa i njihovim procentom, uočenim brojem alela, efektivnim brojem alela, i genetskog diverziteta po Nei -u. UPGMA (unweighted pair group arithmetic mean method) klaster analiza je pokazala da su uzorci bili grupisani u dve glaven podgrupe sa značajnim koeficijentom sličnosti unutar njih. Predstavljeni rezultati pokazuju da RAPD markeri mogu omogućiti analizu genetske divergentnosti u oplemenjivakom materijalu kod koga se očekuje povećana homologija i stepen homozigotnosti.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika-Belgrade",
title = "Estimation of genetic variation among related sugar beet genotypes by using RAPD, Ocena genetske varijabilnosti između srodnih genotipova šećerne repe pomoću RAPD markera",
pages = "582-575",
number = "3",
volume = "43",
doi = "10.2298/GENSR1103575N"
}

Nagl, N., Taški-Ajduković, K., Popović, A., Ćurčić, Ž., Danojević, D.,& Kovačev, L.. (2011). Estimation of genetic variation among related sugar beet genotypes by using RAPD. in Genetika-Belgrade
Društvo genetičara Srbije, Beograd., 43(3), 575-582.
https://doi.org/10.2298/GENSR1103575N

Nagl N, Taški-Ajduković K, Popović A, Ćurčić Ž, Danojević D, Kovačev L. Estimation of genetic variation among related sugar beet genotypes by using RAPD. in Genetika-Belgrade. 2011;43(3):575-582.
doi:10.2298/GENSR1103575N .

Nagl, Nevena, Taški-Ajduković, Ksenija, Popović, Andrea, Ćurčić, Živko, Danojević, Dario, Kovačev, Lazar, "Estimation of genetic variation among related sugar beet genotypes by using RAPD" in Genetika-Belgrade, 43, no. 3 (2011):575-582,
https://doi.org/10.2298/GENSR1103575N . .