TY  - CONF
AU  - Lazić, S.
AU  - Šunjka, D.
AU  - Milovanović, I
AU  - Grahovac, Nada
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/3724
AB  - Non-point-source contamination of surface water has emerged as an important environmental problem during the last decades. This is particularly obvious in areas with intensive pesticide application. Considering importance of water and soil quality, pesticide residues in our environment need to be more effectively documented. This study has focused on determination of pesticide residues in drainage water. Monitoring of pesticide residues were conducted at different locations in the northern part of Serbia, the main agricultural area of the country. The sampling was performed during spring and summer 2012 on twelve potential risk sites. The extraction of pesticides from water was carried out with ENVI™ C18 DSK 47 mm (Supelco 5-7171, Bellafonte, PA). Prior to extraction disc was conditioned with 5 ml of methanol and 5 ml of ultrapure water. Afterward, water sample was filtered through the disc under vacuum and disc was dried for 1 h at 25 °C. Pesticides were eluted from the disc with 5 ml of mixture dichloromethane/n-hexane (40/60, v/v) and evaporated to dryness. The extract was reconstituted in 1 ml of methanol, ultrasonically homogenized and analyzed. Analyses were performed on Agilent Technologies GC-MS Model 7890 A Series gas chromatograph coupled to 5975 C mass selective detector. A HP5MS (30 mx 0.25 mmi.d.) fused silica capillary column was used. Helium was used as the carrier gas at a constant pressure. Injection volume was 2.0 ul, in splitless mode at 280 °C. The total run time was 41.86 min. The interface was kept at 250 °C, the quadropole at 150 °C and the mass spectra were obtained at electron energy of 70 eV. After validation and measurement uncertainty evaluation steps, results obtained showed that the method can be applied to efficiently for monitoring of pesticide residues in water samples. In analyzed samples 22 pesticides were detected -8fungicides,9 herbicides and 5 insecticides. Herbicides were most frequently detected (in 41% of the samples), followed by fungicides (36%) and insecticides (23%)
C3  - 14th European Meeting on Enviromental Chemistry, Budva, 4 December 2013
T1  - Screening of pesticide residues in canal water in the Northern Serbia
EP  - 134
SP  - 134
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3724
ER  - 

@conference{
author = "Lazić, S. and Šunjka, D. and Milovanović, I and Grahovac, Nada",
year = "2013",
abstract = "Non-point-source contamination of surface water has emerged as an important environmental problem during the last decades. This is particularly obvious in areas with intensive pesticide application. Considering importance of water and soil quality, pesticide residues in our environment need to be more effectively documented. This study has focused on determination of pesticide residues in drainage water. Monitoring of pesticide residues were conducted at different locations in the northern part of Serbia, the main agricultural area of the country. The sampling was performed during spring and summer 2012 on twelve potential risk sites. The extraction of pesticides from water was carried out with ENVI™ C18 DSK 47 mm (Supelco 5-7171, Bellafonte, PA). Prior to extraction disc was conditioned with 5 ml of methanol and 5 ml of ultrapure water. Afterward, water sample was filtered through the disc under vacuum and disc was dried for 1 h at 25 °C. Pesticides were eluted from the disc with 5 ml of mixture dichloromethane/n-hexane (40/60, v/v) and evaporated to dryness. The extract was reconstituted in 1 ml of methanol, ultrasonically homogenized and analyzed. Analyses were performed on Agilent Technologies GC-MS Model 7890 A Series gas chromatograph coupled to 5975 C mass selective detector. A HP5MS (30 mx 0.25 mmi.d.) fused silica capillary column was used. Helium was used as the carrier gas at a constant pressure. Injection volume was 2.0 ul, in splitless mode at 280 °C. The total run time was 41.86 min. The interface was kept at 250 °C, the quadropole at 150 °C and the mass spectra were obtained at electron energy of 70 eV. After validation and measurement uncertainty evaluation steps, results obtained showed that the method can be applied to efficiently for monitoring of pesticide residues in water samples. In analyzed samples 22 pesticides were detected -8fungicides,9 herbicides and 5 insecticides. Herbicides were most frequently detected (in 41% of the samples), followed by fungicides (36%) and insecticides (23%)",
journal = "14th European Meeting on Enviromental Chemistry, Budva, 4 December 2013",
title = "Screening of pesticide residues in canal water in the Northern Serbia",
pages = "134-134",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3724"
}

Lazić, S., Šunjka, D., Milovanović, I.,& Grahovac, N.. (2013). Screening of pesticide residues in canal water in the Northern Serbia. in 14th European Meeting on Enviromental Chemistry, Budva, 4 December 2013, 134-134.
https://hdl.handle.net/21.15107/rcub_fiver_3724

Lazić S, Šunjka D, Milovanović I, Grahovac N. Screening of pesticide residues in canal water in the Northern Serbia. in 14th European Meeting on Enviromental Chemistry, Budva, 4 December 2013. 2013;:134-134.
https://hdl.handle.net/21.15107/rcub_fiver_3724 .

Lazić, S., Šunjka, D., Milovanović, I, Grahovac, Nada, "Screening of pesticide residues in canal water in the Northern Serbia" in 14th European Meeting on Enviromental Chemistry, Budva, 4 December 2013 (2013):134-134,
https://hdl.handle.net/21.15107/rcub_fiver_3724 .

TY  - CONF
AU  - Lazić, Sanja
AU  - Šunjka, Dragana B.
AU  - Milovanović, I.
AU  - Jovanov, Pavle
AU  - Grahovac, Nada
PY  - 2013
UR  - http://fiver.ifvcns.rs/handle/123456789/1211
AB  - Pesticides are used worldwide within agriculture to protect crops and ensure the quantity and quality of the harvest. However, intensive and inappropriate pesticides application can directly or indirectly affect different parts of the environment, especially water source. Besides the occurrence of pesticides in drinking water, control of pesticide presence in surface and groundwater is also very important. This primarily refers to the drainage water, river and groundwater, considering the importance of environmental protection and food safety production. The presence of pesticide residues in these matrices may cause yield reduction and decrease product quality, due to its uptake. Objectives of this study included drainage water investigations to identify residue levels of selected pesticides. The sampling was performed during June 2012. Water samples were taken from drainage canals, on twelve sampling points, in intensively cultivated regions of Serbia, Vojvodina Province. Pesticides were chosen based on the European Union Directive 2008/105/EC. This directive by Annex X defines the List of priority substances in the field of water policy. List includes 33 pollutant - 9 are pesticides. The extraction of pesticides from water was performed using 018 ENVI (TM) disc (47 mm). Prior to extraction disc was conditioned with 5 ml of methanol and 5 ml of deionized water. Afterward, water sample was filtered through the disc under vacuum. After drying under vacuum (25 degrees C for 1 h), pesticides were eluted from the disc with 5 ml of mixture of dichloromethane/n-hexane (40/60, v/v) and evaporated to dryness. The extract was dissolved in 1 ml of methanol, ultrasonically homogenized and analyzed. The pesticide residues concentrations analyzed by gas chromatography/electron capture detection with Ni-63. Identification is performed by the use of GC/MS. The mean recovery of this extraction method for all analyzed pesticides at 1 mu g/ml, 0.1 pg/ml and 0.01 pg/ml spiking levels was 93.6%, with associated standard deviations (RSD) of 2.7. The limit of quantification was 0.01 pg/ml. Method accuracy was quantified through measurement uncertainty estimate based on method validation data. The combined relative uncertainty for water samples was 0.481%, while the expanded uncertainty (Us) calculated as U-c=k*U-c, where k is the coverage factor with level of confidence of approximately 95% considering a coverage factor of 2 (EURACHEM/CITAC, 2000), was 0.962%. Obtained values of evaluated analytical parameters are completely in accordance with regulations for analysis of pesticides trace level. Described method was applied for determination of above mentioned pesticides in real water samples. Finally, acetochlor, alachlor and chlorpyrifos were detected in drainage water samples collected in the agricultural area of Serbia.
PB  - Global Nest, Secretariat, Athens
C3  - Proceedings of the 13th International Conference on Environmental Science & Technology
T1  - Determination of pesticide residues in drainage water
UR  - https://hdl.handle.net/21.15107/rcub_fiver_1211
ER  - 

@conference{
author = "Lazić, Sanja and Šunjka, Dragana B. and Milovanović, I. and Jovanov, Pavle and Grahovac, Nada",
year = "2013",
abstract = "Pesticides are used worldwide within agriculture to protect crops and ensure the quantity and quality of the harvest. However, intensive and inappropriate pesticides application can directly or indirectly affect different parts of the environment, especially water source. Besides the occurrence of pesticides in drinking water, control of pesticide presence in surface and groundwater is also very important. This primarily refers to the drainage water, river and groundwater, considering the importance of environmental protection and food safety production. The presence of pesticide residues in these matrices may cause yield reduction and decrease product quality, due to its uptake. Objectives of this study included drainage water investigations to identify residue levels of selected pesticides. The sampling was performed during June 2012. Water samples were taken from drainage canals, on twelve sampling points, in intensively cultivated regions of Serbia, Vojvodina Province. Pesticides were chosen based on the European Union Directive 2008/105/EC. This directive by Annex X defines the List of priority substances in the field of water policy. List includes 33 pollutant - 9 are pesticides. The extraction of pesticides from water was performed using 018 ENVI (TM) disc (47 mm). Prior to extraction disc was conditioned with 5 ml of methanol and 5 ml of deionized water. Afterward, water sample was filtered through the disc under vacuum. After drying under vacuum (25 degrees C for 1 h), pesticides were eluted from the disc with 5 ml of mixture of dichloromethane/n-hexane (40/60, v/v) and evaporated to dryness. The extract was dissolved in 1 ml of methanol, ultrasonically homogenized and analyzed. The pesticide residues concentrations analyzed by gas chromatography/electron capture detection with Ni-63. Identification is performed by the use of GC/MS. The mean recovery of this extraction method for all analyzed pesticides at 1 mu g/ml, 0.1 pg/ml and 0.01 pg/ml spiking levels was 93.6%, with associated standard deviations (RSD) of 2.7. The limit of quantification was 0.01 pg/ml. Method accuracy was quantified through measurement uncertainty estimate based on method validation data. The combined relative uncertainty for water samples was 0.481%, while the expanded uncertainty (Us) calculated as U-c=k*U-c, where k is the coverage factor with level of confidence of approximately 95% considering a coverage factor of 2 (EURACHEM/CITAC, 2000), was 0.962%. Obtained values of evaluated analytical parameters are completely in accordance with regulations for analysis of pesticides trace level. Described method was applied for determination of above mentioned pesticides in real water samples. Finally, acetochlor, alachlor and chlorpyrifos were detected in drainage water samples collected in the agricultural area of Serbia.",
publisher = "Global Nest, Secretariat, Athens",
journal = "Proceedings of the 13th International Conference on Environmental Science & Technology",
title = "Determination of pesticide residues in drainage water",
url = "https://hdl.handle.net/21.15107/rcub_fiver_1211"
}

Lazić, S., Šunjka, D. B., Milovanović, I., Jovanov, P.,& Grahovac, N.. (2013). Determination of pesticide residues in drainage water. in Proceedings of the 13th International Conference on Environmental Science & Technology
Global Nest, Secretariat, Athens..
https://hdl.handle.net/21.15107/rcub_fiver_1211

Lazić S, Šunjka DB, Milovanović I, Jovanov P, Grahovac N. Determination of pesticide residues in drainage water. in Proceedings of the 13th International Conference on Environmental Science & Technology. 2013;.
https://hdl.handle.net/21.15107/rcub_fiver_1211 .

Lazić, Sanja, Šunjka, Dragana B., Milovanović, I., Jovanov, Pavle, Grahovac, Nada, "Determination of pesticide residues in drainage water" in Proceedings of the 13th International Conference on Environmental Science & Technology (2013),
https://hdl.handle.net/21.15107/rcub_fiver_1211 .