TY  - CONF
AU  - Lazić, S.
AU  - Grahovac, Nada
AU  - Šunjka, D.
AU  - Pucarevic, M.
AU  - Jakšić, Snežana
AU  - Popović, Vera
PY  - 2012
UR  - http://fiver.ifvcns.rs/handle/123456789/3726
AB  - Chlorpyrifos is one of organophosphorus (OPPs) insecticides, which are the most important and widely used classes of agricultural pesticides. Because of its intensive use of ecosystems are contaminated by chlorpyrifos and public concerns on safety have been increased.
The method has been developed for confirmation and quantitation of the chlorpyrifos in water samples reverse phase high performance liquid chromatography (HPLC) with photodiodes detection and chromatograms were extracted at 300 nm. This method was single-lab validate in order to demonstrate its performance for monitoring of the insecticide chlorpyrifos in water.
Method accuracy was quantified through measurement uncertainty estimate based on method validation data. Tap and groundwater samples were used to demonstrate linearity and accuracy. Linearity for chlorpyrifos was evaluated by calibration curves in the range of 0.01—10 pg/ml. The accuracy of the proposed method was evaluated as the mean recovery. The analytical method allowed us to quantify chlorpyrifos in tap water and groundwater in concentration level between 0.01-—10 pg/ml with recovery values between of 80.72-109.57 % for tap water and 89.99-97.45 % for groundwater and repeatability lower or equal than 0.6% for both the matrices. The experiments showed that there were no interference peaks from the water matrix (tap and ground) on the elution region of the chlorpyrifos. The RSD values were satisfactory being in the ranges of 0.283-1.237 % (for tap water) and 0.681-3.887 % (for groundwater). The variation of the retention time was less than 0.05 % during these experimental series. The combined relative uncertainty for water samples was 0.481 % (for tap water) and 0.654 % (for groundwater). The expanded uncertainty (Uc) was calculated as Uc= k*u,, where k is the coverage factor with level of confidence of approximately 95% considering a coverage factor of 2 (EURACHEMICITAC, 2000).
We proved that the method was specific for determination of chlorpyrifos in the relevant matrices. After validation and measurement uncertainty evaluation steps, results obtained showed that the method can be applied to efficiently for monitoring of chlorpyrifos in water samples.
PB  - Beograd : Društvo za zaštitu bilja Srbije
C3  - Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012
T1  - Method validation and uncertainty evaluation of the insecticide chlorpyrifos in water samples by using HPLC analysis
EP  - 73
SP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3726
ER  - 

@conference{
author = "Lazić, S. and Grahovac, Nada and Šunjka, D. and Pucarevic, M. and Jakšić, Snežana and Popović, Vera",
year = "2012",
abstract = "Chlorpyrifos is one of organophosphorus (OPPs) insecticides, which are the most important and widely used classes of agricultural pesticides. Because of its intensive use of ecosystems are contaminated by chlorpyrifos and public concerns on safety have been increased.
The method has been developed for confirmation and quantitation of the chlorpyrifos in water samples reverse phase high performance liquid chromatography (HPLC) with photodiodes detection and chromatograms were extracted at 300 nm. This method was single-lab validate in order to demonstrate its performance for monitoring of the insecticide chlorpyrifos in water.
Method accuracy was quantified through measurement uncertainty estimate based on method validation data. Tap and groundwater samples were used to demonstrate linearity and accuracy. Linearity for chlorpyrifos was evaluated by calibration curves in the range of 0.01—10 pg/ml. The accuracy of the proposed method was evaluated as the mean recovery. The analytical method allowed us to quantify chlorpyrifos in tap water and groundwater in concentration level between 0.01-—10 pg/ml with recovery values between of 80.72-109.57 % for tap water and 89.99-97.45 % for groundwater and repeatability lower or equal than 0.6% for both the matrices. The experiments showed that there were no interference peaks from the water matrix (tap and ground) on the elution region of the chlorpyrifos. The RSD values were satisfactory being in the ranges of 0.283-1.237 % (for tap water) and 0.681-3.887 % (for groundwater). The variation of the retention time was less than 0.05 % during these experimental series. The combined relative uncertainty for water samples was 0.481 % (for tap water) and 0.654 % (for groundwater). The expanded uncertainty (Uc) was calculated as Uc= k*u,, where k is the coverage factor with level of confidence of approximately 95% considering a coverage factor of 2 (EURACHEMICITAC, 2000).
We proved that the method was specific for determination of chlorpyrifos in the relevant matrices. After validation and measurement uncertainty evaluation steps, results obtained showed that the method can be applied to efficiently for monitoring of chlorpyrifos in water samples.",
publisher = "Beograd : Društvo za zaštitu bilja Srbije",
journal = "Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012",
title = "Method validation and uncertainty evaluation of the insecticide chlorpyrifos in water samples by using HPLC analysis",
pages = "73-73",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3726"
}

Lazić, S., Grahovac, N., Šunjka, D., Pucarevic, M., Jakšić, S.,& Popović, V.. (2012). Method validation and uncertainty evaluation of the insecticide chlorpyrifos in water samples by using HPLC analysis. in Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012
Beograd : Društvo za zaštitu bilja Srbije., 73-73.
https://hdl.handle.net/21.15107/rcub_fiver_3726

Lazić S, Grahovac N, Šunjka D, Pucarevic M, Jakšić S, Popović V. Method validation and uncertainty evaluation of the insecticide chlorpyrifos in water samples by using HPLC analysis. in Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012. 2012;:73-73.
https://hdl.handle.net/21.15107/rcub_fiver_3726 .

Lazić, S., Grahovac, Nada, Šunjka, D., Pucarevic, M., Jakšić, Snežana, Popović, Vera, "Method validation and uncertainty evaluation of the insecticide chlorpyrifos in water samples by using HPLC analysis" in Annual MGPR Meeting 2012 and International Conference on Food and Health Safety: Moving Towards a Sustainable Agriculture, Belgrade, 11 October 2012 (2012):73-73,
https://hdl.handle.net/21.15107/rcub_fiver_3726 .

TY  - JOUR
AU  - Lazić, S.
AU  - Pucarevic, M.
AU  - Bursić, V.
AU  - Ostojić, Nada
AU  - Vuković, S.
PY  - 2009
UR  - http://fiver.ifvcns.rs/handle/123456789/3716
AB  - Our study comprised the determination of the pesticide residue content in 108 samples of
apples. The pesticide residues were determined by gas chromatography with NPD, ECD and GLC - MS.
The samples were iested for the content of 75 pesticides with LOD of 0.001 to 0.005 mg/kg which were
lower than MRLs according to the EU standards. The relative standard deviation was lower than 19% for
all the tested compounds. In the apple samples taken during 2004, the percentage of the samples positive to the pesticide residue content was 51.85% and the most frequent contaminants were *} HCH and
endosulfan. In the analyzed samples, taken during 2005, out of 70.91% samples in which the pesticide
residues were detected, endosulfan was present in 41.03%, captan in 35.9%, chlorpyrifos in 20.51% and
parathion in 17.95%. The content of the procymidone and lambda-cyhalothrin residues were over the EU
MALs. During 2006 all apple samples were residue-free. Out of 13 samples analyzed in 2007, five contained the pesticide residues below the EU MRLs with the most frequently detected dithiocarbamate.
T2  - Plant Science (Bulgaria)
T1  - Pesticide residues in apple samples on the market of Republic of Serbia 2004-2007
EP  - 310
IS  - 4
SP  - 306
VL  - 46
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3716
ER  - 

@article{
author = "Lazić, S. and Pucarevic, M. and Bursić, V. and Ostojić, Nada and Vuković, S.",
year = "2009",
abstract = "Our study comprised the determination of the pesticide residue content in 108 samples of
apples. The pesticide residues were determined by gas chromatography with NPD, ECD and GLC - MS.
The samples were iested for the content of 75 pesticides with LOD of 0.001 to 0.005 mg/kg which were
lower than MRLs according to the EU standards. The relative standard deviation was lower than 19% for
all the tested compounds. In the apple samples taken during 2004, the percentage of the samples positive to the pesticide residue content was 51.85% and the most frequent contaminants were *} HCH and
endosulfan. In the analyzed samples, taken during 2005, out of 70.91% samples in which the pesticide
residues were detected, endosulfan was present in 41.03%, captan in 35.9%, chlorpyrifos in 20.51% and
parathion in 17.95%. The content of the procymidone and lambda-cyhalothrin residues were over the EU
MALs. During 2006 all apple samples were residue-free. Out of 13 samples analyzed in 2007, five contained the pesticide residues below the EU MRLs with the most frequently detected dithiocarbamate.",
journal = "Plant Science (Bulgaria)",
title = "Pesticide residues in apple samples on the market of Republic of Serbia 2004-2007",
pages = "310-306",
number = "4",
volume = "46",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3716"
}

Lazić, S., Pucarevic, M., Bursić, V., Ostojić, N.,& Vuković, S.. (2009). Pesticide residues in apple samples on the market of Republic of Serbia 2004-2007. in Plant Science (Bulgaria), 46(4), 306-310.
https://hdl.handle.net/21.15107/rcub_fiver_3716

Lazić S, Pucarevic M, Bursić V, Ostojić N, Vuković S. Pesticide residues in apple samples on the market of Republic of Serbia 2004-2007. in Plant Science (Bulgaria). 2009;46(4):306-310.
https://hdl.handle.net/21.15107/rcub_fiver_3716 .

Lazić, S., Pucarevic, M., Bursić, V., Ostojić, Nada, Vuković, S., "Pesticide residues in apple samples on the market of Republic of Serbia 2004-2007" in Plant Science (Bulgaria), 46, no. 4 (2009):306-310,
https://hdl.handle.net/21.15107/rcub_fiver_3716 .

TY  - CONF
AU  - Pucarevic, M.
AU  - Sekulić, P.
AU  - Vasin, Jovica
AU  - Ostojić, Nada
AU  - Milošević, N.
PY  - 2008
UR  - http://fiver.ifvcns.rs/handle/123456789/3686
AB  - The objective of this study was to assess the effect of oil drilling waste on
the environment. This paper reviews the results of analyses of sotl and ground water
taken from the two oil-drilling sites situated near the Vojvoda Stepa village tn eastern
part of Banat region. A total of 29 soil samples and 5 surface and ground water samples have been analyzed.
In the location of Vojvoda Stepa | (VS1) and Vojvoda Stepa 2 (VS2) total petroleum
hydrocarbons (TPH) ranged from 1, 54 g/kg to 5, 98 g/kg and 6, 26 g/kg to SS, 94 g/ke
respectively. The soils in the location VS2 should be adequately recultivated in order tt
lower the TPH to the aceptable level of 2. 5 ofke.
TPH concentrations in water ranged from 6,8 mg/d? to 44, 3 myfdm. The methoc
detection limit for water was 5 mg/dnv. US standards for ground water specify that the
water more than 100 m away from a possible source of potable walter may Contain it}
to 50 mgldne af TPH, while the water potentially usable for water supply should no
have more than | mg/dm’ of TPH (CERL Special Report, 2000). If we apply this stan
dard, there is no risk for water supply since there are no settlements near the analyzec
sites.
C3  - 2. Međunarodna naučna konferencija Remedijacija, stanje i perspektive u zaštiti životne sredine
T1  - Hydrocarbons at the  Vojvoda Stepa oil drilling sites
EP  - 54
IS  - 45
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3686
ER  - 

@conference{
author = "Pucarevic, M. and Sekulić, P. and Vasin, Jovica and Ostojić, Nada and Milošević, N.",
year = "2008",
abstract = "The objective of this study was to assess the effect of oil drilling waste on
the environment. This paper reviews the results of analyses of sotl and ground water
taken from the two oil-drilling sites situated near the Vojvoda Stepa village tn eastern
part of Banat region. A total of 29 soil samples and 5 surface and ground water samples have been analyzed.
In the location of Vojvoda Stepa | (VS1) and Vojvoda Stepa 2 (VS2) total petroleum
hydrocarbons (TPH) ranged from 1, 54 g/kg to 5, 98 g/kg and 6, 26 g/kg to SS, 94 g/ke
respectively. The soils in the location VS2 should be adequately recultivated in order tt
lower the TPH to the aceptable level of 2. 5 ofke.
TPH concentrations in water ranged from 6,8 mg/d? to 44, 3 myfdm. The methoc
detection limit for water was 5 mg/dnv. US standards for ground water specify that the
water more than 100 m away from a possible source of potable walter may Contain it}
to 50 mgldne af TPH, while the water potentially usable for water supply should no
have more than | mg/dm’ of TPH (CERL Special Report, 2000). If we apply this stan
dard, there is no risk for water supply since there are no settlements near the analyzec
sites.",
journal = "2. Međunarodna naučna konferencija Remedijacija, stanje i perspektive u zaštiti životne sredine",
title = "Hydrocarbons at the  Vojvoda Stepa oil drilling sites",
pages = "54",
number = "45",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3686"
}

Pucarevic, M., Sekulić, P., Vasin, J., Ostojić, N.,& Milošević, N.. (2008). Hydrocarbons at the  Vojvoda Stepa oil drilling sites. in 2. Međunarodna naučna konferencija Remedijacija, stanje i perspektive u zaštiti životne sredine(45).
https://hdl.handle.net/21.15107/rcub_fiver_3686

Pucarevic M, Sekulić P, Vasin J, Ostojić N, Milošević N. Hydrocarbons at the  Vojvoda Stepa oil drilling sites. in 2. Međunarodna naučna konferencija Remedijacija, stanje i perspektive u zaštiti životne sredine. 2008;(45):null-54.
https://hdl.handle.net/21.15107/rcub_fiver_3686 .

Pucarevic, M., Sekulić, P., Vasin, Jovica, Ostojić, Nada, Milošević, N., "Hydrocarbons at the  Vojvoda Stepa oil drilling sites" in 2. Međunarodna naučna konferencija Remedijacija, stanje i perspektive u zaštiti životne sredine, no. 45 (2008),
https://hdl.handle.net/21.15107/rcub_fiver_3686 .

TY  - CONF
AU  - Pucarevic, M.
AU  - Ostojić, Nada
AU  - Jevtić, Radivoje
PY  - 2007
UR  - http://fiver.ifvcns.rs/handle/123456789/3711
AB  - Primena mešavine više pesticida u jednom tanku je odavno u upotrebi. Za razblaženje pesticida se koristi česmenska, površinska ili podzemna voda. Kod ovakvog načina primene važno je postupiti po preporučenoj proceduri proizvođača. Međutim, uočeni su izvesni problemi. Najčešće je to pojava taloga, flokula i drugih agregata koji nakon umešavanja otežavaju aplikaciju. U radu je prikazan uticaj elektroprovodijivosti, ukupne tvrdoće, temperature i pH vrednosti vode koja se koristi za pripremanje tank miksa na zapreminu taloga nastalog mešanjem dva fungicida Bravo 720 SC (hlorotalonil 720g/L) i Rias 300 EC (difenokonazol + propikonazol 150 + 150 g/L). Primena ova dva preparata je ustaljena u praksi za suzbijanje prouzrokovača sive pegavosti lista šećerne repe (Cercospora beticola).
C3  - Zbornik rezimea radova, 13. Simpozijum sa savetovanjem o zaštiti bilja, Zlatibor, 26-30.11.2007.
T1  - Zavisnost taloga mešavine fungicida Bravo 720 SC i Rias 300-EC od elektroprovodljivosti, ukupne tvrdoće i pH vrednosti vode
EP  - 148
SP  - 147
UR  - https://hdl.handle.net/21.15107/rcub_fiver_3711
ER  - 

@conference{
author = "Pucarevic, M. and Ostojić, Nada and Jevtić, Radivoje",
year = "2007",
abstract = "Primena mešavine više pesticida u jednom tanku je odavno u upotrebi. Za razblaženje pesticida se koristi česmenska, površinska ili podzemna voda. Kod ovakvog načina primene važno je postupiti po preporučenoj proceduri proizvođača. Međutim, uočeni su izvesni problemi. Najčešće je to pojava taloga, flokula i drugih agregata koji nakon umešavanja otežavaju aplikaciju. U radu je prikazan uticaj elektroprovodijivosti, ukupne tvrdoće, temperature i pH vrednosti vode koja se koristi za pripremanje tank miksa na zapreminu taloga nastalog mešanjem dva fungicida Bravo 720 SC (hlorotalonil 720g/L) i Rias 300 EC (difenokonazol + propikonazol 150 + 150 g/L). Primena ova dva preparata je ustaljena u praksi za suzbijanje prouzrokovača sive pegavosti lista šećerne repe (Cercospora beticola).",
journal = "Zbornik rezimea radova, 13. Simpozijum sa savetovanjem o zaštiti bilja, Zlatibor, 26-30.11.2007.",
title = "Zavisnost taloga mešavine fungicida Bravo 720 SC i Rias 300-EC od elektroprovodljivosti, ukupne tvrdoće i pH vrednosti vode",
pages = "148-147",
url = "https://hdl.handle.net/21.15107/rcub_fiver_3711"
}

Pucarevic, M., Ostojić, N.,& Jevtić, R.. (2007). Zavisnost taloga mešavine fungicida Bravo 720 SC i Rias 300-EC od elektroprovodljivosti, ukupne tvrdoće i pH vrednosti vode. in Zbornik rezimea radova, 13. Simpozijum sa savetovanjem o zaštiti bilja, Zlatibor, 26-30.11.2007., 147-148.
https://hdl.handle.net/21.15107/rcub_fiver_3711

Pucarevic M, Ostojić N, Jevtić R. Zavisnost taloga mešavine fungicida Bravo 720 SC i Rias 300-EC od elektroprovodljivosti, ukupne tvrdoće i pH vrednosti vode. in Zbornik rezimea radova, 13. Simpozijum sa savetovanjem o zaštiti bilja, Zlatibor, 26-30.11.2007.. 2007;:147-148.
https://hdl.handle.net/21.15107/rcub_fiver_3711 .

Pucarevic, M., Ostojić, Nada, Jevtić, Radivoje, "Zavisnost taloga mešavine fungicida Bravo 720 SC i Rias 300-EC od elektroprovodljivosti, ukupne tvrdoće i pH vrednosti vode" in Zbornik rezimea radova, 13. Simpozijum sa savetovanjem o zaštiti bilja, Zlatibor, 26-30.11.2007. (2007):147-148,
https://hdl.handle.net/21.15107/rcub_fiver_3711 .