TY  - JOUR
AU  - Imerovski, Ivana
AU  - Dedić, Boško
AU  - Cvejić, Sandra
AU  - Miladinović, Dragana
AU  - Jocić, Siniša
AU  - Owens, Gregory L.
AU  - Kočiš-Tubić, Nataša
AU  - Rieseberg, Loren H.
PY  - 2019
UR  - http://fiver.ifvcns.rs/handle/123456789/1933
AB  - Broomrape (Orobanche cumana) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F-3 families were phenotyped for resistance to broomrape race G. Bulk segregant analyses (BSA) combined with genotyping-by-sequencing (GBS) technology was used to verify previously identified genes and map new resistance QTLs. Resistance had a polygenic basis, and numerous QTLs were found in all mapping populations. Contributing components to resistance that were common to all populations mapped to two major QTLs on chromosome 3, which were designated or3.1 and or3.2. QTL or3.1 was positioned in a genomic region where a previous broomrape resistance gene Or5 has been mapped, while QTL or3.2 was identified for the first time in the lower region of the same chromosome. Following the identification of major QTLs for resistance using the BSA-seq approach, all plants from three F-2 populations were genotyped using newly developed CAPS markers nearest to the QTL peak for or3.2, which confirmed the association of these markers with broomrape race G resistance. The results of this study will bring us a step closer to understanding the mechanisms underlying resistance of sunflower to highly virulent broomrape races, and the molecular markers developed herein will be highly useful for sunflower breeding programs.
PB  - Springer, Dordrecht
T2  - Molecular Breeding
T1  - BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines
IS  - 3
VL  - 39
DO  - 10.1007/s11032-019-0948-9
ER  - 

@article{
author = "Imerovski, Ivana and Dedić, Boško and Cvejić, Sandra and Miladinović, Dragana and Jocić, Siniša and Owens, Gregory L. and Kočiš-Tubić, Nataša and Rieseberg, Loren H.",
year = "2019",
abstract = "Broomrape (Orobanche cumana) is a parasitic weed that causes substantial yield losses in sunflower. In this study, four biparental genetic populations comprised of between 96 and 150 F-3 families were phenotyped for resistance to broomrape race G. Bulk segregant analyses (BSA) combined with genotyping-by-sequencing (GBS) technology was used to verify previously identified genes and map new resistance QTLs. Resistance had a polygenic basis, and numerous QTLs were found in all mapping populations. Contributing components to resistance that were common to all populations mapped to two major QTLs on chromosome 3, which were designated or3.1 and or3.2. QTL or3.1 was positioned in a genomic region where a previous broomrape resistance gene Or5 has been mapped, while QTL or3.2 was identified for the first time in the lower region of the same chromosome. Following the identification of major QTLs for resistance using the BSA-seq approach, all plants from three F-2 populations were genotyped using newly developed CAPS markers nearest to the QTL peak for or3.2, which confirmed the association of these markers with broomrape race G resistance. The results of this study will bring us a step closer to understanding the mechanisms underlying resistance of sunflower to highly virulent broomrape races, and the molecular markers developed herein will be highly useful for sunflower breeding programs.",
publisher = "Springer, Dordrecht",
journal = "Molecular Breeding",
title = "BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines",
number = "3",
volume = "39",
doi = "10.1007/s11032-019-0948-9"
}

Imerovski, I., Dedić, B., Cvejić, S., Miladinović, D., Jocić, S., Owens, G. L., Kočiš-Tubić, N.,& Rieseberg, L. H.. (2019). BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines. in Molecular Breeding
Springer, Dordrecht., 39(3).
https://doi.org/10.1007/s11032-019-0948-9

Imerovski I, Dedić B, Cvejić S, Miladinović D, Jocić S, Owens GL, Kočiš-Tubić N, Rieseberg LH. BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines. in Molecular Breeding. 2019;39(3).
doi:10.1007/s11032-019-0948-9 .

Imerovski, Ivana, Dedić, Boško, Cvejić, Sandra, Miladinović, Dragana, Jocić, Siniša, Owens, Gregory L., Kočiš-Tubić, Nataša, Rieseberg, Loren H., "BSA-seq mapping reveals major QTL for broomrape resistance in four sunflower lines" in Molecular Breeding, 39, no. 3 (2019),
https://doi.org/10.1007/s11032-019-0948-9 . .

TY  - JOUR
AU  - Imerovski, Ivana
AU  - Dimitrijević, Aleksandra
AU  - Miladinović, Dragana
AU  - Dedić, Boško
AU  - Jocić, Siniša
AU  - Kočiš-Tubić, Nataša
AU  - Cvejić, Sandra
PY  - 2016
UR  - http://fiver.ifvcns.rs/handle/123456789/1549
AB  - Broomrape (Orobanche cumana) is one of the most important parasitic plants that can drastically reduce sunflower yield. Breeding for resistance is regarded as the most effective and environmentally friendly control measure. Due to the constant changes in broomrape race composition, i.e. the emergence of races F and higher, the majority of the existing resistance genes have become insufficient for crop protection. In this paper we report the genetic analysis and mapping of a new resistance gene for broomrape races higher than F. Broomrape resistance was evaluated using F-1 plants and F-2:3 families derived from a cross between the resistant sunflower inbred line AB-VL-8 and the susceptible line L-OS-1. The results showed that F-1 plants were susceptible, indicating a recessive resistance. In the derived F-2 population and F-3 families, the resistance segregation deviated significantly from the one-gene Mendelian ratio. However, marker analysis revealed polymorphism only on LG3, indicating that presumably single gene in this region conferred the resistance. The closest marker to the gene, tentatively designated as or (ab-vl-8), was ORS683 with the genetic distance of 1.5 centimorgans. The discovery of or (ab-vl-8) will provide a much needed new sunflower resistance gene for new broomrape races, and the associated markers will facilitate the introgression of the gene into different sunflower lines.
PB  - Springer, Dordrecht
T2  - Euphytica
T1  - Mapping of a new gene for resistance to broomrape races higher than F
EP  - 289
IS  - 2
SP  - 281
VL  - 209
DO  - 10.1007/s10681-015-1597-7
ER  - 

@article{
author = "Imerovski, Ivana and Dimitrijević, Aleksandra and Miladinović, Dragana and Dedić, Boško and Jocić, Siniša and Kočiš-Tubić, Nataša and Cvejić, Sandra",
year = "2016",
abstract = "Broomrape (Orobanche cumana) is one of the most important parasitic plants that can drastically reduce sunflower yield. Breeding for resistance is regarded as the most effective and environmentally friendly control measure. Due to the constant changes in broomrape race composition, i.e. the emergence of races F and higher, the majority of the existing resistance genes have become insufficient for crop protection. In this paper we report the genetic analysis and mapping of a new resistance gene for broomrape races higher than F. Broomrape resistance was evaluated using F-1 plants and F-2:3 families derived from a cross between the resistant sunflower inbred line AB-VL-8 and the susceptible line L-OS-1. The results showed that F-1 plants were susceptible, indicating a recessive resistance. In the derived F-2 population and F-3 families, the resistance segregation deviated significantly from the one-gene Mendelian ratio. However, marker analysis revealed polymorphism only on LG3, indicating that presumably single gene in this region conferred the resistance. The closest marker to the gene, tentatively designated as or (ab-vl-8), was ORS683 with the genetic distance of 1.5 centimorgans. The discovery of or (ab-vl-8) will provide a much needed new sunflower resistance gene for new broomrape races, and the associated markers will facilitate the introgression of the gene into different sunflower lines.",
publisher = "Springer, Dordrecht",
journal = "Euphytica",
title = "Mapping of a new gene for resistance to broomrape races higher than F",
pages = "289-281",
number = "2",
volume = "209",
doi = "10.1007/s10681-015-1597-7"
}

Imerovski, I., Dimitrijević, A., Miladinović, D., Dedić, B., Jocić, S., Kočiš-Tubić, N.,& Cvejić, S.. (2016). Mapping of a new gene for resistance to broomrape races higher than F. in Euphytica
Springer, Dordrecht., 209(2), 281-289.
https://doi.org/10.1007/s10681-015-1597-7

Imerovski I, Dimitrijević A, Miladinović D, Dedić B, Jocić S, Kočiš-Tubić N, Cvejić S. Mapping of a new gene for resistance to broomrape races higher than F. in Euphytica. 2016;209(2):281-289.
doi:10.1007/s10681-015-1597-7 .

Imerovski, Ivana, Dimitrijević, Aleksandra, Miladinović, Dragana, Dedić, Boško, Jocić, Siniša, Kočiš-Tubić, Nataša, Cvejić, Sandra, "Mapping of a new gene for resistance to broomrape races higher than F" in Euphytica, 209, no. 2 (2016):281-289,
https://doi.org/10.1007/s10681-015-1597-7 . .