@conference{
author = "Stamenov, Dragana and Đurić, Simonida and Hajnal Jafari, Timea and Kiprovski, Biljana and Seman, Vladimira",
year = "2021",
abstract = "The objective of this study was the isolation and characterization of Azotobacter, Pseudomonas
and Bacillus species from the rhizospheric soil of Mellisa officinalis and to examine the effect of
isolates application on the seed germination and seedling growth. Isolation, physiological,
biochemical, and plant-growth-promoting activity characterization of isolates were done.
Monitoring the effects of isolates application on seed germination and seedling growth were
evaluated in controlled conditions. The number of germinated seeds, the shoot and root length of
seedlings, vigour index and biochemical stress markers (lipid peroxidation intensity and total
phenols) were measured. From the rhizosphere of plant 2 Azotobacter (denoted as A5 and A6), 5
Pseudomonas (denoted as P27, P28, P29, P55 and P56) and 6 Bacillus (denoted as B64, B65,
B66, B67, B68 and B69) bacteria were isolated. Azotobacter isolates showed the ability to live in
the condition of low (5) and high (9) pH. The isolates varied in terms of the utilization of carbon
sources. Both isolates produced pectinase, hydrogen cyanide (HCN) and utilized organic and
inorganic phosphorus. Pseudomonas isolates had optimal growth at 10 ºC and 37 ºC, and on
medium with pH 5 and 9. All Pseudomonas isolates could produce pectinase, lipase, amylose,
IAA, siderophores and HCN. All Bacillus isolates could grow on a medium containing 7% NaCl.",
publisher = "East Sarajevo : Faculty of Agriculture",
journal = "Book of Abstracts, 12th International scientific agriculture symposium "Agrosym 2021", Jahorina, 07-10 October 2021",
title = "Azotobacter, Pseudomonas and Bacillus isolates stimulate the germination and seedling growth of Mellisa officinalis",
pages = "200-200",
url = "https://hdl.handle.net/21.15107/rcub_fiver_2329"
}
