TY  - CONF
AU  - Mitrović, Ivana
AU  - Tančić-Živanov, Sonja
AU  - Purar, Božana
AU  - Trivunović, Zorana
AU  - Mitrović, Bojan
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2530
AB  - Maize is an agricultural crop that is susceptible to infections by various phytopathogenic fungi, producers of mycotoxins harmful to humans and animals. Since this agricultural crop has an important place in the human diet, its health safety is very important. Trichoderma genus has great potential in the biocontrol of various phytopathogens however, the medium composition as well as the cultivation conditions, have a significant impact on the efficiency of the produced Trichoderma bioagents. In this work, influence of medium pH, temperature and mixing speed on the productivity of Trichoderma bioagent effective against two maize pathogens, Fusarium graminearum and Aspergillus flavus, was investigated. The results obtained by statistical processing show that the best productivity of Trichoderma harzianum bioagent was achieved when the pH of the medium was 6, the temperature was 28°C and the mixing speed of the rotary shaker was 180 rpm. By applying these cultivation conditions, the largest inhibition zone diameters of F. graminearum and A. flavus mycelial growth were formed. Also, the results show that the maize pathogen, F. graminearum, was more sensitive to the produced Trichoderma biocontrol agent compared to the other maize pathogen, A. flavus.
PB  - Tuzla: Univerzitet u Tuzli, Tehnološki fakultet
C3  - Book of Abstracts, OPORPH 2021, 7th International scientific-professional symposium “Environmental resources, sustainable development and food production”,12 November 2021, Tuzla, Bosnia and Herzegovina
T1  - Influence of process parameters on the production of Trichoderma biocontrol agent
EP  - 8
VL  - 8
DO  - 10.5281/zenodo.5656338
ER  - 

@conference{
author = "Mitrović, Ivana and Tančić-Živanov, Sonja and Purar, Božana and Trivunović, Zorana and Mitrović, Bojan",
year = "2021",
abstract = "Maize is an agricultural crop that is susceptible to infections by various phytopathogenic fungi, producers of mycotoxins harmful to humans and animals. Since this agricultural crop has an important place in the human diet, its health safety is very important. Trichoderma genus has great potential in the biocontrol of various phytopathogens however, the medium composition as well as the cultivation conditions, have a significant impact on the efficiency of the produced Trichoderma bioagents. In this work, influence of medium pH, temperature and mixing speed on the productivity of Trichoderma bioagent effective against two maize pathogens, Fusarium graminearum and Aspergillus flavus, was investigated. The results obtained by statistical processing show that the best productivity of Trichoderma harzianum bioagent was achieved when the pH of the medium was 6, the temperature was 28°C and the mixing speed of the rotary shaker was 180 rpm. By applying these cultivation conditions, the largest inhibition zone diameters of F. graminearum and A. flavus mycelial growth were formed. Also, the results show that the maize pathogen, F. graminearum, was more sensitive to the produced Trichoderma biocontrol agent compared to the other maize pathogen, A. flavus.",
publisher = "Tuzla: Univerzitet u Tuzli, Tehnološki fakultet",
journal = "Book of Abstracts, OPORPH 2021, 7th International scientific-professional symposium “Environmental resources, sustainable development and food production”,12 November 2021, Tuzla, Bosnia and Herzegovina",
title = "Influence of process parameters on the production of Trichoderma biocontrol agent",
pages = "8",
volume = "8",
doi = "10.5281/zenodo.5656338"
}

Mitrović, I., Tančić-Živanov, S., Purar, B., Trivunović, Z.,& Mitrović, B.. (2021). Influence of process parameters on the production of Trichoderma biocontrol agent. in Book of Abstracts, OPORPH 2021, 7th International scientific-professional symposium “Environmental resources, sustainable development and food production”,12 November 2021, Tuzla, Bosnia and Herzegovina
Tuzla: Univerzitet u Tuzli, Tehnološki fakultet., 8.
https://doi.org/10.5281/zenodo.5656338

Mitrović I, Tančić-Živanov S, Purar B, Trivunović Z, Mitrović B. Influence of process parameters on the production of Trichoderma biocontrol agent. in Book of Abstracts, OPORPH 2021, 7th International scientific-professional symposium “Environmental resources, sustainable development and food production”,12 November 2021, Tuzla, Bosnia and Herzegovina. 2021;8:null-8.
doi:10.5281/zenodo.5656338 .

Mitrović, Ivana, Tančić-Živanov, Sonja, Purar, Božana, Trivunović, Zorana, Mitrović, Bojan, "Influence of process parameters on the production of Trichoderma biocontrol agent" in Book of Abstracts, OPORPH 2021, 7th International scientific-professional symposium “Environmental resources, sustainable development and food production”,12 November 2021, Tuzla, Bosnia and Herzegovina, 8 (2021),
https://doi.org/10.5281/zenodo.5656338 . .

TY  - JOUR
AU  - Mitrović, Ivana
AU  - Tančić-Živanov, Sonja
AU  - Purar, Božana
AU  - Trivunović, Zorana
AU  - Mitrović, Bojan
PY  - 2021
UR  - http://fiver.ifvcns.rs/handle/123456789/2527
AB  - The increasing usage of chemicals for plant protection in recent years has become a serious problem. One of the possible solutions is use of beneficial microorganisms instead of synthetic fungicides, which will contribute to the protection of the environment and human health. Since the fungi Aspergillus flavus and Fusarium graminearum are the most important pathogens that cause maize diseases and produce mycotoxins, the potential of Trichoderma harzianum for biocontrol of both phytopathogens was examined in this paper. The aim of this paper was to study the influence of different carbon and nitrogen combinations in the medium for T. harzianum production. T. harzianum was cultivated in Erlenmeyer flasks and the effect of cultivation broth against selected maize pathogens was tested using well diffusion method. The results of this study showed that the combination of different carbon and nitrogen sources in the T. harzianum cultivation medium statistically significantly affects the production of Trichoderma cultivation broth effective on two tested phytopathogens. Dextrose as a carbon source and soybean flour as a nitrogen source proved to be the best combination in the medium for production of T. harzianum cultivation broth effective on A. flavus and F. graminearum. Maximal inhibition zone diameters of 31 mm and 56.33 mm were registered in those medium formulations for A. flavus and F. graminearum, respectively. These researches represent an important step for further research in which a medium of low market value would be selected. This would reduce the price of the production process but also the final product.
AB  - Sve veća upotreba hemijskih sredstava za zaštitu bilja poslednjih godina postala je ozbiljan problem. Upotreba korisnih mikroorganizama umesto sintetičkih fungicida u biološkoj zaštiti sigurno je jedno od rešenja koje doprinose zaštiti životne sredine i ljudskog zdravlja. S obzirom na to da se gljive Aspergillus flavus i Fusarium graminearum pominju kao najvažniji prouzrokovači bolesti kukuruza, proizvođači mikotoksina, u ovom radu ispitan je antifungalni efekat Trichoderma harzianum na obe fitopatogene gljive. Cilj ovog rada je ispitati uticaj različitih kombinacija ugljenika i azota u podlozi za proizvodnju T. harzianum. Kultivacija T. harzianum izvedena je u erlenmajerima na rotacionoj tresilici, a aktivnost dobijene kultivacione tečnosti na odabrane fitopatogene kukuruza ispitana je difuzionom metodom sa bunarima. Rezultati ovog rada pokazali su da kombinacija različitih izvora ugljenika i azota u podlozi statistički značajno utiče na proizvodnju Trichoderma kultivacione tečnosti efikasne na dve testirane fitopatogene gljive. Kombinacija koja sadrži dekstrozu kao izvor ugljenika i sojino brašno kao izvor azota pokazala se najboljom za formulaciju podloge za proizvodnju T. harzianum kultivacione tečnosti efikasne na patogene kukuruza, A. flavus i F. graminearum, formirajući maksimalne prečnike zona inhibicije od 31 mm, odnosno 56,33 mm.
PB  - Novi Sad: Institut za ratarstvo i povrtarstvo
T2  - Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
T1  - Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum
T1  - Uticaj različitih kombinacija izvora ugljenika i azota u podlozi za proizvodnju biokontrolnog agensa Trichoderma harzianum
EP  - 6
IS  - 1
SP  - 1
VL  - 58
DO  - 10.5937/ratpov58-29961
ER  - 

@article{
author = "Mitrović, Ivana and Tančić-Živanov, Sonja and Purar, Božana and Trivunović, Zorana and Mitrović, Bojan",
year = "2021",
abstract = "The increasing usage of chemicals for plant protection in recent years has become a serious problem. One of the possible solutions is use of beneficial microorganisms instead of synthetic fungicides, which will contribute to the protection of the environment and human health. Since the fungi Aspergillus flavus and Fusarium graminearum are the most important pathogens that cause maize diseases and produce mycotoxins, the potential of Trichoderma harzianum for biocontrol of both phytopathogens was examined in this paper. The aim of this paper was to study the influence of different carbon and nitrogen combinations in the medium for T. harzianum production. T. harzianum was cultivated in Erlenmeyer flasks and the effect of cultivation broth against selected maize pathogens was tested using well diffusion method. The results of this study showed that the combination of different carbon and nitrogen sources in the T. harzianum cultivation medium statistically significantly affects the production of Trichoderma cultivation broth effective on two tested phytopathogens. Dextrose as a carbon source and soybean flour as a nitrogen source proved to be the best combination in the medium for production of T. harzianum cultivation broth effective on A. flavus and F. graminearum. Maximal inhibition zone diameters of 31 mm and 56.33 mm were registered in those medium formulations for A. flavus and F. graminearum, respectively. These researches represent an important step for further research in which a medium of low market value would be selected. This would reduce the price of the production process but also the final product., Sve veća upotreba hemijskih sredstava za zaštitu bilja poslednjih godina postala je ozbiljan problem. Upotreba korisnih mikroorganizama umesto sintetičkih fungicida u biološkoj zaštiti sigurno je jedno od rešenja koje doprinose zaštiti životne sredine i ljudskog zdravlja. S obzirom na to da se gljive Aspergillus flavus i Fusarium graminearum pominju kao najvažniji prouzrokovači bolesti kukuruza, proizvođači mikotoksina, u ovom radu ispitan je antifungalni efekat Trichoderma harzianum na obe fitopatogene gljive. Cilj ovog rada je ispitati uticaj različitih kombinacija ugljenika i azota u podlozi za proizvodnju T. harzianum. Kultivacija T. harzianum izvedena je u erlenmajerima na rotacionoj tresilici, a aktivnost dobijene kultivacione tečnosti na odabrane fitopatogene kukuruza ispitana je difuzionom metodom sa bunarima. Rezultati ovog rada pokazali su da kombinacija različitih izvora ugljenika i azota u podlozi statistički značajno utiče na proizvodnju Trichoderma kultivacione tečnosti efikasne na dve testirane fitopatogene gljive. Kombinacija koja sadrži dekstrozu kao izvor ugljenika i sojino brašno kao izvor azota pokazala se najboljom za formulaciju podloge za proizvodnju T. harzianum kultivacione tečnosti efikasne na patogene kukuruza, A. flavus i F. graminearum, formirajući maksimalne prečnike zona inhibicije od 31 mm, odnosno 56,33 mm.",
publisher = "Novi Sad: Institut za ratarstvo i povrtarstvo",
journal = "Ratarstvo i povrtarstvo / Field and Vegetable Crops Research",
title = "Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum, Uticaj različitih kombinacija izvora ugljenika i azota u podlozi za proizvodnju biokontrolnog agensa Trichoderma harzianum",
pages = "6-1",
number = "1",
volume = "58",
doi = "10.5937/ratpov58-29961"
}

Mitrović, I., Tančić-Živanov, S., Purar, B., Trivunović, Z.,& Mitrović, B.. (2021). Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research
Novi Sad: Institut za ratarstvo i povrtarstvo., 58(1), 1-6.
https://doi.org/10.5937/ratpov58-29961

Mitrović I, Tančić-Živanov S, Purar B, Trivunović Z, Mitrović B. Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum. in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research. 2021;58(1):1-6.
doi:10.5937/ratpov58-29961 .

Mitrović, Ivana, Tančić-Živanov, Sonja, Purar, Božana, Trivunović, Zorana, Mitrović, Bojan, "Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum" in Ratarstvo i povrtarstvo / Field and Vegetable Crops Research, 58, no. 1 (2021):1-6,
https://doi.org/10.5937/ratpov58-29961 . .